LOCUS       BGC0000521             12132 bp    DNA     linear   BCT 26-JUL-2016
DEFINITION  Lactococcus lactis subsp. lactis plasmid pES2 transposon Tn5721 and
            lacticin 481 operon, complete sequence; and unknown genes.
ACCESSION   BGC0000521
VERSION     BGC0000521
KEYWORDS    .
SOURCE      Lactococcus lactis subsp. lactis
  ORGANISM  Lactococcus lactis subsp. lactis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Lactococcus.
COMMENT     On Nov 6, 1998 this sequence version replaced gi:2581772.
            ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 13:46:56
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(<1..498)
                     /codon_start=1
                     /note="orf8"
                     /product="unknown"
                     /protein_id="AAC72260.1"
                     /transl_table=11
                     /translation="MFKTKKFKEIELENELLKNKIENASFSPDRFINKIKDLIESNDFS
                     SVKYLIKTLTDYLTLKEATHCLLRNNTHSTDKNDMLLWTIYGLYRNFDVKTIDCDLKFS
                     INELNLILNTWNEDRIVSNIKGINSTNILYPCNNIRNYYIYPLDIVICGGGNHSQFSAK
                     LKL"
     source          1..12132
                     /db_xref="taxon:1360"
                     /mol_type="genomic DNA"
                     /organism="Lactococcus lactis subsp. lactis"
                     /plasmid="pES2"
                     /strain="C2102"
                     /sub_species="lactis"
     protocluster    1..12132
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[2763:5532]"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      2764..5532
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
     cand_cluster    1..12132
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(LANC_like and DUF4135)"
                     /kind="single"
                     /product="lanthipeptide-class-ii"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..12132
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000521"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..12132
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lanthipeptide-class-ii"
                     /region_number="1"
                     /rules="cds(LANC_like and DUF4135)"
                     /subregion_numbers="1"
                     /tool="antismash"
     repeat_region   584..610
                     /rpt_type=inverted
     mobile_element  584..2189
                     /mobile_element_type="insertion sequence:IS1675"
     mobile_element  584..11840
                     /mobile_element_type="transposon:Tn5721"
     gene            737..2056
                     /gene="tpase"
     CDS             737..2056
                     /codon_start=1
                     /gene="tpase"
                     /product="putative transposase"
                     /protein_id="AAC72261.1"
                     /transl_table=11
                     /translation="MLNTTKVPSTLQVSHQIKKNLEDQIHEITNHPEIYAQSPFDFSRN
                     RKLSFETTIKIILSFGGQSLSSELLSHFNFTLKTPTASALVQARSKIKLKAFEQLFYRT
                     IPSAQPNKLYKGYRIFAHDGSDLNIPYNEKESDTHYRVGKFGKHVGSLHLNALYDPLNK
                     HYVAVDFQKIKQLNERKSLCQIVDDFDFTSPTIIIADRGYESFNVYEHIKKSGQKFLIR
                     AKDTKSNGLLNGLDLPSDGTFDKKITLQLTRRQTNKVKKDKHYHFLHKRANFDYLPIRS
                     KETYPISLRVVRIKLNEDTYESLVTNLDPFLFTSEDLKVLYHLRWGIETSFRELKYALG
                     LSHFHSKKLDFIIQEIFARLIMYNFSMTITLAVVLSNRLKHSYQINFTQAFGICRRFFL
                     DQNVNVEQLISRYLLPIRPNRSDQRRLIKKKFPGFLYRIA"
     repeat_region   2163..2189
                     /rpt_type=inverted
     gene            2471..2626
                     /gene="lctA"
                     /operon="lacticin 481"
     CDS             2471..2626
                     /codon_start=1
                     /gene="lctA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) L_biotic_A"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) leader_d"
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /operon="lacticin 481"
                     /product="preprolacticin 481"
                     /protein_id="AAC72257.1"
                     /sec_met_domain="L_biotic_A (E-value: 6.6e-31, bitscore:
                     95.5, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="leader_d (E-value: 4.9e-08, bitscore:
                     22.5, seeds: 16, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKEQNSFNLLQEVTESELDLILGAKGGSGVIHTISHECNMNSWQF
                     VFTCCS"
     CDS_motif       2471..2542
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence: MKEQNSFNLLQEVTESELDLILGA"
                     /locus_tag="lctA_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       2543..2626
                     /prepeptide="core"
                     /locus_tag="lctA_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="1.80"
                     /molecular_weight="2865.2"
                     /monoisotopic_mass="2863.3"
                     /core_sequence="KGGSGVIHTISHECNMNSWQFVFTCCS"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MKEQNSFNLLQEVTESELDLILGA"
                     /leader_location="[2470:2542](+)"
                     /alternative_weights="2883.2"
                     /alternative_weights="2901.3"
                     /alternative_weights="2919.3"
     operon          2471..10087
                     /operon="lacticin 481"
     mat_peptide     2543..2623
                     /function="class I bacteriocin; lantibiotic"
                     /gene="lctA"
                     /note="formerly lactococcin DR"
                     /product="lacticin 481"
     gene            2764..5532
                     /gene="lctM"
                     /operon="lacticin 481"
     CDS             2764..5532
                     /codon_start=1
                     /function="posttranslational modifications of
                     preprolactococin 481"
                     /gene="lctM"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: DUF4135"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: LANC_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1070:lanthionine synthetase C family protein (Score:
                     757.3; E-value: 4.1e-229)"
                     /gene_kind="biosynthetic"
                     /operon="lacticin 481"
                     /product="LctM"
                     /protein_id="AAC72258.1"
                     /sec_met_domain="DUF4135 (E-value: 2.2e-83, bitscore:
                     269.8, seeds: 79, tool: rule-based-clusters)"
                     /sec_met_domain="LANC_like (E-value: 6.3e-78, bitscore:
                     251.3, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKKTYQFEKFLKNTFDQFSIKQNEVLVEDDLNDIIMNVCGKALV
                     LMINEKREMNLLMGNTPEERYQYFENEYSSTGKAFEEIKDKFPVIYIDLKNSINSYLKL
                     VSQIMKDFKKDYSLLVERKIIEEHSTISTMKIKGDLHNGKAVIEITTNKSKLIYKPKSL
                     SNDVFFNNFLKYMDSFFIKEGKSTKYKENFYLVNTLDMKTYGWVEYVDKKPINSFEEAR
                     NYYRKIGVLLSVAYTLNLTDLHFENVISQGENPCIIDLETMFNMPMFVKDYKNESRNII
                     NGKIMDSVVSTGMLPVLGIDSLFGGDPSGILGGTFSKEERVIINPFRDDIKFQKIVVRS
                     VFKDHIPFFNNNNEKRYCKPKDYVNDIIKGFEKTYKIIVKNKEKILGFLKKESSSVTCR
                     ILFRNTMEYSVLLNAAKSPVYSNKREEIFEKLSTFNRGLGNDIIKSEISQINTLSIPYF
                     NCQVDSNLIKNMDGETIFEHTLTPFKCFLSKYRRLCVDDMEQQVKLIRFSIQSQEQLFK
                     DGEQFSLYKKQKGSQEDLLIAINELSSILENNAYIGTSDDTINWMSLGIADNDQILFES
                     LENDIYKGISGIGLALLEYYEFYPNINTKKILKLIYKNISKDFINTNNEPQNYGFYVGL
                     IGEYSFLRKYEKVFHKTSSCNILKNILKDFTPEKCQTILPSDDVIAGEAGIIIYISNLN
                     NYLEYRDEIDILLKSLSNKIKLKESIASYAHGNSGIATAFVHGYKVTKNEKYLKIFHEL
                     WNLENSSKLRRGWTDSRKVDSSYSSQWCHGASGQAIARMEWITVNKTARFLSNSELIKV
                     KKELGELIDILKKEGMYTDNFCLCHGILGNLLILNTYQENFDNKNINLKNEILNNYYSV
                     CNYGLNKGWICGLGTEFYSYGLMTGISGILYGLIRQVKQKNNFGVLMPYVD"
     gene            5551..7626
                     /gene="lctT"
                     /operon="lacticin 481"
     CDS             5551..7626
                     /codon_start=1
                     /function="protease; ATP-dependent transport protein;
                     cleavage of the leader peptide and export of mature
                     lacticin 481"
                     /gene="lctT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 144.1; E-value:
                     9.5e-44)"
                     /gene_kind="transport"
                     /operon="lacticin 481"
                     /product="LctT"
                     /protein_id="AAC72259.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 1.7e-41, bitscore:
                     130.6, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIVLQNNEQDCLLACYSMILGYFGRDVAIHELYSGEMIPPDGLS
                     VSYLKNINMKHQVSMHVYKTDKKNSPNKIFYPKMLPVIIQWNDNHFVVVTKIYRKNVTL
                     IDPAIGKVKYNYNDFMKKFSGYIITLSPNSSFTKKKRISEIIFPLKKIFKNRNTFLYIF
                     SLFISQIVALWFSIILRDILNKSHDITYSFIMMISLVLFQTLSLLMKLGAQKNTNLLYE
                     SKISRQIFKGIFSRPLLYFRNNSVGTIIEKINLRTGIRDGILLKIFPSLLNFFTVFIVI
                     IYLGTISFTLTLFLVIMNLLYMIFSFSLISIKRQANIQYTQQTIDFTSVVQEDLNQIEQ
                     IKAQANEKECVKRWTKKSAQTIFSYNKILNIDGITSAFNQGFNYICVILMMIFGIYLNQ
                     GNLVSIPDLIIFQSGISLFVSAVNQIQDVMFEISRLSIYGNKISDLLIENPQRIDNIEK
                     HSNNAIILKDISYSYELNNYIFNNINFSIKKGEKIAIVGKSGSGKSTLFNILLGLISYE
                     GEVTYGYENLRQIIGVVSQNMNLRKGSLIENIVSNNNSEELDIQKINDVLKDVNMLELV
                     DSLPQKIFSQLFENGKNLSGGQIQRLLIAKSLLNNNKFIFWDEPFSSLDNQNRIHIYKN
                     VLENPDYKSQTIIMISHHLDVLKYVDRVIYIDDKKIMIDKHNNLLLNDSYNSFVNE"
     gene            7684..8604
                     /gene="lctF"
                     /operon="lacticin 481"
     CDS             7684..8604
                     /codon_start=1
                     /function="immunity to the bacteriocin lacticin 481"
                     /gene="lctF"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 176.5; E-value:
                     1.1e-53)"
                     /gene_kind="transport"
                     /note="ABC transporter (ATP-binding domain) homolog"
                     /operon="lacticin 481"
                     /product="LctF"
                     /protein_id="AAC72253.1"
                     /transl_table=11
                     /translation="MHIEKILETKKLSKKFGKEYSVRNLTISVKKGDIFGFLGPNGAGK
                     STTMKMILGLLHPTEGEVKVFGKKLDNSVKNSVLKDVGCLIEEPSFYPNLSGLENLKLV
                     QKLLNLPYHNIEKVLNIVKLEDQKNKLVKNYSLGMKQRLGIALALIKFPKLLILDEPTN
                     GLDPTGIREIRELIKSLPEKYGITVLISSHLLSEIEQIANTVAIIKKGELLYEGELSKL
                     EEQPIFEIQTNLINQSFEVLQGLGYTVKKQSNDTIQIMNSDLNKRAAAIEALVSQQIKI
                     YEAKSVTKNLEQVFIEMTSDLERKF"
     gene            8604..9356
                     /gene="lctE"
                     /operon="lacticin 481"
     CDS             8604..9356
                     /codon_start=1
                     /function="immunity to the bacteriocin lacticin 481"
                     /gene="lctE"
                     /note="ABC transporter (trans-membrane domain) homolog"
                     /operon="lacticin 481"
                     /product="LctE"
                     /protein_id="AAC72254.1"
                     /transl_table=11
                     /translation="MLKYIYLELLKTKRRRFFSPLIIFFVVGLLWSSVLFVKEYPLPYS
                     GDKIVILINDLTIINSMFSPLIIGIMCSNLFSLEHDNQTFKVLQTNGQSIENLFYAKTI
                     FAFLIILIFGLFQFFYINLIAILFKIGDPIYLSFLYFIGYIIASLFLIILHLFISLKIR
                     KQSLGIIISVIASFVGLVSGGMLPLYLKFIIPWQYFSLLSPIHREFKSNSFLYSINKNF
                     NILVLCIFILIIIELIVINRRIRRLNLC"
     gene            9350..10087
                     /gene="lctG"
                     /operon="lacticin 481"
     CDS             9350..10087
                     /codon_start=1
                     /function="proposed to be associated to LctF and LctE to
                     form an ABC transporter involved in immunity to lacticin
                     481"
                     /gene="lctG"
                     /note="ABC transporter (trans-membrane domain) homolog"
                     /operon="lacticin 481"
                     /product="LctG"
                     /protein_id="AAC72255.1"
                     /transl_table=11
                     /translation="MLKDLILFEFIKIKNSKILFIIFFFNIFSVLIGSVIYLSNLEFLK
                     NENQLSLVLWGQCSLYSSQIFLPVLIGILCSISWQMEEKNKNWQRMKTIPISDFSFVFS
                     KFLNIQVFNLISSIIYYLLFVISVILLGVPDTPFLLFFIWCIYGWLGTFPITALQLLLA
                     IKFKNFTSPIICSASLAIIGLATLFFGKIIFTIFPYTQINIGIRARVLDNFKPSEFIIF
                     IIVISFYSFIGLFYSSKILKKRE"
     repeat_region   10235..10261
                     /rpt_type=inverted
     mobile_element  10235..11840
                     /mobile_element_type="insertion sequence:IS1675"
     gene            10388..11707
                     /gene="tpase"
     CDS             10388..11707
                     /codon_start=1
                     /gene="tpase_rename1"
                     /note="Orf7"
                     /product="putative transposase"
                     /protein_id="AAC72256.1"
                     /transl_table=11
                     /translation="MLNTTKVPSTLQVSHQIKKNLEDQIHEITNHPEIYAQSPFDFSRN
                     RKLSFETTIKIILSFGGQSLSSELLSHFNFTLKTPTASALVQARSKIKLKAFEQLFYRT
                     IPSAQPNKLYKGYRIFAHDGSDLNIPYNEKESDTHYRVGKFGKHVGSLHLNALYDPLNK
                     HYVAVDFQKIKQLNERKSLCQIVDDFDFTSPTIIIADRGYESFNVYEHIKKSGQKFLIR
                     AKDTKSNGLLNGLDLPSDGTFDKKITLQLTRRQTNKVKKDKHYHFLHKRANFDYLPIRS
                     KETYPISLRVVRIKLNEDTYESLVTNLDPFLFTSEDLKVLYHLRWGIETSFRELKYALG
                     LSHFHSKKLDFIIQEIFARLIMYNFSMTITLAVVLSNRLKHSYQINFTQAFGICRRFFL
                     DQNVNVEQLISRYLLPIRPNRSDQRRLIKKKFPGFLYRIA"
     repeat_region   11814..11840
                     /rpt_type=inverted
     CDS             12029..>12132
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /note="Orf9"
                     /product="unknown"
                     /protein_id="AAC72262.1"
                     /transl_table=11
                     /translation="MKLENLFIEKLHGIYNYDVDFNEDITFLYGSNGCG"
     CDS_motif       12029..12118
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence: MKLENLFIEKLHGIYNYDVDFNEDITFLYG"
                     /locus_tag="AAC72262.1_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       12119..12130
                     /prepeptide="core"
                     /locus_tag="AAC72262.1_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="-4.60"
                     /molecular_weight="418.4"
                     /monoisotopic_mass="418.1"
                     /core_sequence="SNGCG"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MKLENLFIEKLHGIYNYDVDFNEDITFLYG"
                     /leader_location="[12028:12118](+)"
ORIGIN
        1 aagctttaat ttagctgaaa attgagaatg attgccccca ccacaaatca ctatatctaa
       61 aggataaatg taataatttc ttatgttatt acaaggatat aatatattag ttgaatttat
      121 ccctttaata ttactaacta ttctatcctc attccatgta tttagaatta aattcaattc
      181 attaatagaa aattttaaat cgcaatcgat agttttcaca tcaaaatttc tgtaaagacc
      241 atatatagtc catagcaaca tatcattttt atcagtacta tgtgtattat ttcttaataa
      301 acaatgagtt gcttctttca aagtaagata gtcagttaac gttttaatca ggtatttaac
      361 tgaactaaaa tcatttgatt caattaaatc ttttatttta ttgataaacc tatcaggaga
      421 aaaagaagca ttttcaattt tattttttaa aagttcattc tctagttcta tttctttgaa
      481 tttttttgtt ttaaacattt ttccacctct ttttgaacaa gtctattgaa cacctacaac
      541 ccctattata ccaataacta aaaatagttc aatagagtat actccaatgt cattaagtta
      601 aggaaaacag aaaaacagga aggatctaat cacctcctgt tttattttat tctaagcaag
      661 aagaacaccc tcttgataca attattttat caataataaa acgtcaaagc gttacgtatt
      721 ctggagggat gtcctattgc ttaatactac caaagttcca tcaacacttc aagtgtctca
      781 tcaaataaag aaaaacttag aagatcaaat ccacgagatt accaatcatc ctgaaattta
      841 tgctcaatct ccttttgatt tttctagaaa tagaaagtta tcttttgaga caacgattaa
      901 aatcatcctc tcttttggag ggcaaagtct atctagtgaa ttactctcac attttaattt
      961 caccctaaaa acccctaccg cgtctgcttt ggtacaagct cgtagtaaaa taaagttaaa
     1021 agcatttgag caactctttt accgaactat tccttcagca caacccaata aattatacaa
     1081 aggctatcga atatttgccc atgatggttc tgatcttaat atcccttaca atgaaaaaga
     1141 atcagatacc cattatagag ttgggaaatt tgggaagcat gttggatcac ttcaccttaa
     1201 tgctttatat gatcctttaa ataaacacta cgtggcagtg gactttcaaa aaattaagca
     1261 gttaaatgaa cgtaaaagtt tgtgtcagat agttgatgat tttgatttca caagtccaac
     1321 aattattatt gcggatcgtg ggtatgaatc ttttaatgtt tatgaacaca ttaaaaaaag
     1381 cggacaaaaa tttttgattc gagcaaaaga tacaaagagt aatgggttgc ttaatggcct
     1441 tgatttgcca tcagatggaa catttgataa aaaaataacc cttcaattaa caagaagaca
     1501 gacaaataag gtaaaaaaag ataaacatta tcatttcttg cataaaagag cgaattttga
     1561 ttatcttcct atccgctcca aagaaactta tccgatcagt ctcagagtcg ttagaattaa
     1621 gttgaatgag gacacctatg aatccttagt gacaaatctg gatccgttcc tatttacaag
     1681 cgaagaccta aaagtccttt atcacttgcg ttggggaatt gaaacttcct ttagagaatt
     1741 gaaatatgct ctaggtttaa gccacttcca ttcaaagaaa cttgacttta ttatccaaga
     1801 aatctttgcc cgtctcatta tgtataattt ttcaatgaca attactttgg ctgtggtctt
     1861 atctaatcgt ttaaaacact cctaccagat taattttaca caggcctttg ggatttgtag
     1921 gcggtttttc cttgatcaaa acgtcaatgt agagcaattg ataagccgat atcttcttcc
     1981 cataaggccg aatcggagtg atcaaagaag attaataaaa aagaaatttc ctggtttttt
     2041 atatcgtatt gcctaagatt atcaatcgga ctatacaagt aggacgtttt tttgcgtgat
     2101 tcatttatga aattagaacg tcaatgagat agaaaaacaa aatatttaag aataaaatga
     2161 tactgttttc cttaacttaa tgacattgga gcataccctg ttgtccatca ataaaaatct
     2221 tctaaaatta ttttactcaa attgatagat taattttatg aaatgtgttg acatttgtta
     2281 ctatctaaac aatcaaaata ttatataatg atttgactaa ttaatggagg ctccttatga
     2341 acaaaaataa aaccaagttc attgcatctg cactcacttc attagttatt gcattttact
     2401 aatcgaagga gtataatgat ttcgaaggta aggaaatact ttcaaattaa aagtaaggga
     2461 gtgaacaata atgaaagaac aaaactcttt taatcttctt caagaagtga cagaaagtga
     2521 attggacctt attttaggtg caaaaggcgg cagtggagtt attcatacaa tttctcatga
     2581 atgtaatatg aatagctggc aatttgtatt tacttgctgc tcttaatttt attgaaaaga
     2641 aattatattc tatggagcaa tgattaatta ttgctccttt ctttttataa atcgtaatct
     2701 ttagcaaatg ataaaagagg tgtaattacc tttttagatg aatagcaaca taaggacaaa
     2761 atagtgaaaa aaaagactta ccaatttgaa aaatttttaa aaaatacttt tgatcaattt
     2821 tctattaagc aaaatgaagt tctggttgaa gatgatttaa acgatataat tatgaacgtt
     2881 tgtggaaaag cacttgtttt gatgataaat gaaaaaagag aaatgaatct attaatgggc
     2941 aatacaccag aggaaaggta ccaatatttt gaaaatgagt attcgagtac aggtaaagct
     3001 tttgaagaaa taaaagataa atttccagta atatatattg atttaaaaaa ttctataaat
     3061 tcttatttaa agttggtttc acaaataatg aaagatttta aaaaagatta ctcacttcta
     3121 gtagaacgta agattattga ggaacattcg actatttcga ctatgaaaat aaaaggtgat
     3181 ttacataatg ggaaggctgt tatagaaatt actactaaca aaagtaaatt aatttataaa
     3241 ccaaagtcat taagtaatga tgtgtttttt aacaattttt tgaagtatat ggatagtttt
     3301 tttattaaag agggaaaaag cactaaatat aaagaaaatt tttatttggt aaacacgctt
     3361 gatatgaaga catatggatg ggttgaatac gtagataaaa aaccaatcaa ttcatttgag
     3421 gaagcaagaa attattatag aaaaattgga gtacttttat cagttgctta tactttaaat
     3481 ttaactgact tacattttga aaatgtgatc tcacaaggag aaaatccttg tattattgac
     3541 ctagagacta tgtttaacat gcctatgttt gtaaaagatt ataaaaatga atctcgtaat
     3601 attattaatg gaaagattat ggattcggta gtctcaacag gaatgttacc agtcttagga
     3661 atagatagtt tgtttggggg ggatcctagt ggaattttag gtggtacatt ttctaaagaa
     3721 gaacgagtga tcataaatcc atttagagat gacataaaat ttcaaaaaat agttgtacga
     3781 tctgtattca aagatcatat tccttttttt aacaataata atgagaaaag atattgtaag
     3841 cccaaagact atgttaatga tattataaaa gggtttgaaa aaacatataa aataatcgtt
     3901 aaaaataagg aaaaaatatt agggtttcta aaaaaagaat ctagtagtgt tacctgtaga
     3961 atattattta gaaatacgat ggaatactca gttttattaa atgcagcaaa gtcgcctgta
     4021 tattcaaaca aaagagaaga aatttttgaa aaattatcaa cttttaatcg aggacttgga
     4081 aatgatatta ttaaatcaga gataagtcaa ataaacactt tatcaatccc ctatttcaat
     4141 tgtcaagtag actcaaactt aataaagaat atggatggag aaacaatatt tgagcatact
     4201 ctcaccccat tcaaatgttt cctatcaaaa tatagaagac tgtgtgtaga tgatatggaa
     4261 caacaagtta agctaatccg attttcaatt caaagtcaag aacagctttt taaagatggg
     4321 gaacagttca gtttatataa gaaacaaaaa ggttcacaag aagatttatt gattgcgata
     4381 aatgagcttt caagtatctt agaaaacaat gcatatattg gtacaagcga tgataccata
     4441 aattggatga gtttaggaat tgctgataat gatcagatac tctttgaaag tcttgaaaat
     4501 gatatatata agggaatatc aggaataggc ttagccttat tggaatatta tgaattttat
     4561 ccaaatatca acacaaaaaa aatactaaaa ttaatatata aaaatatatc aaaagatttt
     4621 attaatacaa ataatgagcc ccaaaattat ggattctatg ttggcttaat aggtgagtat
     4681 agttttttga gaaaatacga aaaagtattt cacaaaacaa gtagttgcaa cattttaaag
     4741 aacattttaa aagattttac tcccgagaag tgtcaaacaa tactaccttc agatgacgta
     4801 atagccggag aagcgggaat tattatttac atttcaaatc tcaataatta cctagaatac
     4861 agagatgaaa ttgatattct attgaaaagt ttatcaaata agataaaatt aaaagaaagt
     4921 attgcaagtt atgctcatgg taatagtggt atagcaacag cttttgtaca tggatataag
     4981 gttactaaaa atgaaaaata tcttaagata ttccatgaac tttggaattt agaaaattct
     5041 agtaaactga gaagaggttg gacagattca agaaaagttg atagttcata ctcttcacag
     5101 tggtgtcacg gtgcatcggg acaagctata gcaagaatgg agtggattac tgtaaacaaa
     5161 acagctagat ttcttagtaa ctctgaacta attaaggtta aaaaagagct aggggaatta
     5221 attgatatct taaaaaaaga gggaatgtat acagataatt tttgtctatg ccatggtatt
     5281 ttaggaaatc tattaatttt aaatacctat caagagaatt ttgataataa gaatatcaat
     5341 ctaaagaatg aaattttaaa caactattac tctgtttgta actatggttt aaataaagga
     5401 tggatttgtg gcttaggtac agaattttat tcttatgggc ttatgacagg aatatctggt
     5461 atattatatg gactgattcg gcaagtaaaa caaaaaaata attttggagt cttaatgcca
     5521 tatgttgatt aatatcatta ggagtttaga atgaaaatag ttttacaaaa taatgagcag
     5581 gattgtttgc tagcatgcta ttcaatgata ttgggatatt ttggtaggga tgttgcaata
     5641 catgagcttt atagtgggga aatgatcccg cctgatggct tgtctgtttc atatttaaaa
     5701 aatattaata tgaagcatca agttagtatg catgtttata agactgataa aaagaattct
     5761 ccaaataaga tattctatcc aaagatgctg cctgtaatta tacaatggaa tgataatcat
     5821 tttgttgtag taactaagat ttacagaaaa aatgtaacac tcattgaccc tgcaataggt
     5881 aaagtgaagt ataactataa tgattttatg aaaaaatttt ctggttatat tattacttta
     5941 tcaccgaata gttcttttac aaagaaaaaa agaataagtg aaattatctt tccactaaaa
     6001 aaaattttca agaataggaa tacctttctt tatatttttt ctttgtttat ctctcaaatt
     6061 gttgcattat ggttttcaat aatattaaga gatatcttga acaaatctca tgacataaca
     6121 tattctttta ttatgatgat ttccttagtc ctctttcaaa ctttatcact gttaatgaaa
     6181 ctaggggctc aaaaaaatac aaatctatta tatgaatcca aaatatctag acaaattttt
     6241 aaaggaatat ttagtaggcc actattatat tttagaaaca actcagttgg tacaataata
     6301 gaaaaaatta atctaagaac gggaataaga gacggaatac ttctgaaaat ttttccatca
     6361 ctcctaaact ttttcacggt ctttattgta attatatatt tgggaacaat ctcctttaca
     6421 ctgactcttt ttttggtaat aatgaatcta ttatatatga tttttagctt tagtttgatt
     6481 agtatcaaaa gacaggctaa tattcaatat acacaacaaa caattgactt tacatcagtt
     6541 gtgcaagagg atttaaatca gattgaacag ataaaagctc aggcaaatga aaaagaatgt
     6601 gttaaaagat ggacaaaaaa aagcgctcaa acaatattct cttataataa aatcttaaat
     6661 attgacggaa ttacaagtgc tttcaatcag ggcttcaatt acatttgtgt tatattgatg
     6721 atgatatttg gaatatattt aaatcaaggg aatttagttt ctataccaga tttgattatc
     6781 tttcagtcgg gtatttcttt gtttgtttca gctgtcaatc aaattcagga tgttatgttt
     6841 gaaatatcta gattatcaat ctatggtaat aaaataagcg atttactaat tgaaaatcct
     6901 cagagaatag ataatataga aaaacattcc aacaatgcta ttatattgaa agacatctca
     6961 tattcatacg aattaaataa ttatattttt aataacataa atttttcaat taaaaagggt
     7021 gaaaaaatag ctatagttgg aaaatcaggt tcaggtaaaa gcacattatt taatatttta
     7081 ttaggtctaa tatcttatga aggggaagtt acttatggtt atgagaattt acgtcaaata
     7141 atcggagttg tttcacaaaa tatgaattta agaaagggaa gtttaatcga aaatattgta
     7201 tctaacaata attccgaaga actagatatt caaaaaatta atgatgtgtt gaaagatgta
     7261 aacatgttag aattagtaga ctctctccct caaaagatat tctctcaact ttttgaaaat
     7321 gggaaaaatc tttcgggagg acagattcaa cgattattaa tagcaaaatc actattaaat
     7381 aataacaaat ttattttttg ggatgaaccg tttagtagct tagataatca aaatagaata
     7441 catatttata agaacgttct agaaaatccc gattataaat cacaaacaat aattatgatt
     7501 agtcatcatt tggatgtctt gaaatatgtc gatagagtga tttatataga tgacaagaaa
     7561 attatgattg acaaacacaa caacctttta ttaaatgatt cctataattc atttgttaat
     7621 gaataaataa aaagttacgt atcgctgttt taaatttatc aaaaagaaag aattgggtgg
     7681 aatatgcaca ttgaaaaaat cttagagaca aaaaaattaa gtaaaaaatt tggaaaagaa
     7741 tattcagtaa gaaatcttac aatatcagtt aaaaaaggtg acatttttgg ctttcttgga
     7801 cctaatgggg cagggaagag tacaacaatg aaaatgattc taggtttatt gcatcctacc
     7861 gagggagagg taaaagtgtt cggtaagaaa ttagataatt cggttaaaaa ttcggttttg
     7921 aaagatgttg gatgtttgat cgaggaaccg tcattttatc caaatttatc gggtttggaa
     7981 aatctcaagc tagttcaaaa attattaaat cttccttatc ataacattga aaaagtactt
     8041 aatattgtta aactagaaga ccaaaaaaac aaactagtaa aaaattattc acttggaatg
     8101 aaacagagac taggcatagc tttagcatta atcaagttcc caaagttact aattttggat
     8161 gaacctacga atggcttgga tcccactggg ataagggaaa ttcgagaatt aataaaatct
     8221 ttacccgaaa aatacggaat tactgtatta atctctagtc atttattatc agaaatagaa
     8281 caaattgcca atacagtagc aattattaaa aagggagaat tactttatga aggagaattg
     8341 tctaaattag aagagcaacc aatctttgaa attcaaacaa atttaatcaa tcaatctttc
     8401 gaagttttac aaggacttgg atatacagtc aaaaaacaat ctaatgacac tattcaaatc
     8461 atgaactctg acttaaataa aagagctgct gctatagaag ctttagtaag tcaacaaatt
     8521 aaaatatatg aagcaaaatc tgtaactaaa aatctagagc aagtatttat tgaaatgact
     8581 tcagatcttg aaaggaaatt ttaatgctga aatacatcta cttagaactg ttaaaaacaa
     8641 aaagacgccg ctttttttca ccactgataa tattttttgt tgtcggacta ctatggtcgt
     8701 ctgtactatt tgtgaaggaa tacccattac catatagtgg ggataaaata gtaatcttaa
     8761 tcaatgattt gacgatcata aatagtatgt tttcgccact tattatagga attatgtgtt
     8821 ccaacttgtt ttcacttgag catgataatc aaactttcaa agtgttacaa acaaatggtc
     8881 aaagtattga aaacttattt tatgctaaaa caatctttgc ttttttgatt atattgattt
     8941 ttggtttatt ccagtttttt tatataaatt taattgccat tttatttaaa attggagatc
     9001 cgatttatct ttctttttta tattttatag gatatattat agcttctctt tttttaatca
     9061 ttctgcatct cttcatatct ctaaaaatca gaaaacaatc tcttggtatc ataatatctg
     9121 ttattgccag ctttgtgggt ttagtatctg gaggaatgtt gccactttat cttaaattca
     9181 ttattccttg gcagtatttt tcccttttaa gtccaataca cagagaattt aaaagtaatt
     9241 cttttttata ttctattaac aaaaatttta atatcttagt tttatgtata tttattttaa
     9301 tcatcataga attaatagtt attaatagaa gaattaggag gttgaacctt tgttaaaaga
     9361 tcttattctt tttgaattta tcaaaattaa gaatagtaaa atattattta ttattttctt
     9421 tttcaatatc tttagtgtat taattgggag tgtaatttat ctttcaaatt tggaattctt
     9481 aaaaaatgaa aaccagctaa gtttggtatt gtggggacaa tgttcattat atagctctca
     9541 aatatttctt ccagtattaa ttggaatatt atgttcgatt agttggcaaa tggaagaaaa
     9601 aaataaaaac tggcagagga tgaaaacgat acctatttct gattttagtt ttgtcttttc
     9661 aaaattttta aatatacaag tatttaattt gataagtagc ataatttact acttattgtt
     9721 tgttattagc gttattcttc taggggtacc tgatacgcca tttcttttat tttttatttg
     9781 gtgtatttac ggatggttag gtacgtttcc aattactgct ctccaacttt tattagcaat
     9841 aaaatttaaa aatttcacct ccccaataat atgttcggcc tcacttgcta ttattggttt
     9901 ggctacgctt ttttttggta aaataatttt cacaatattt ccgtatactc aaataaatat
     9961 aggaattaga gctagagtat tagataattt taaaccatct gagtttataa tctttataat
    10021 tgtgatatca ttttattcat tcataggttt attttattcc tctaaaatat taaaaaaaag
    10081 agaatgaatt cagttgaagt tatgttgaga ttgttgaaaa attaaagcat gaaatctctt
    10141 ctatgtcaac taattggaat ttttaataaa actgatatca tccttaaatc ttagattatg
    10201 tatcatataa ttaaaaaggc caccagagta tagcccaatg tcattaagtt aaggaaaaca
    10261 gaaaaacagg aaggatctaa tcacctcctg ttttatttta ttctaagcaa gaagaacacc
    10321 ctcttgatac aattatttta tcaataataa aacgtcaaag cgttacgtat tctggaggga
    10381 tgtcctattg cttaatacta ccaaagttcc atcaacactt caagtgtctc atcaaataaa
    10441 gaaaaactta gaagatcaaa tccacgagat taccaatcat cctgaaattt atgctcaatc
    10501 tccttttgat ttttctagaa atagaaagtt atcttttgag acaacgatta aaatcatcct
    10561 ctcttttgga gggcaaagtc tatctagtga attactctca cattttaatt tcaccctaaa
    10621 aacccctacc gcgtctgctt tggtacaagc tcgtagtaaa ataaagttaa aagcatttga
    10681 gcaactcttt taccgaacta ttccttcagc acaacccaat aaattataca aaggctatcg
    10741 aatatttgcc catgatggtt ctgatcttaa tatcccttac aatgaaaaag aatcagatac
    10801 ccattataga gttgggaaat ttgggaagca tgttggatca cttcacctta atgctttata
    10861 tgatccttta aataaacact acgtggcagt ggactttcaa aaaattaagc agttaaatga
    10921 acgtaaaagt ttgtgtcaga tagttgatga ttttgatttc acaagtccaa caattattat
    10981 tgcggatcgt gggtatgaat cttttaatgt ttatgaacac attaaaaaaa gcggacaaaa
    11041 atttttgatt cgagcaaaag atacaaagag taatgggttg cttaatggcc ttgatttgcc
    11101 atcagatgga acatttgata aaaaaataac ccttcaatta acaagaagac agacaaataa
    11161 ggtaaaaaaa gataaacatt atcatttctt gcataaaaga gcgaattttg attatcttcc
    11221 tatccgctcc aaagaaactt atccgatcag tctcagagtc gttagaatta agttgaatga
    11281 ggacacctat gaatccttag tgacaaatct ggatccgttc ctatttacaa gcgaagacct
    11341 aaaagtcctt tatcacttgc gttggggaat tgaaacttcc tttagagaat tgaaatatgc
    11401 tctaggttta agccacttcc attcaaagaa acttgacttt attatccaag aaatctttgc
    11461 ccgtctcatt atgtataatt tttcaatgac aattactttg gctgtggtct tatctaatcg
    11521 tttaaaacac tcctaccaga ttaattttac acaggccttt gggatttgta ggcggttttt
    11581 ccttgatcaa aacgtcaatg tagagcaatt gataagccga tatcttcttc ccataaggcc
    11641 gaatcggagt gatcaaagaa gattaataaa aaagaaattt cctggttttt tatatcgtat
    11701 tgcctaagat tatcaatcgg actatacaag taggacgttt ttttgcgtga ttcatttatg
    11761 aaattagaac gtcaatgaga tagaaaaaca aaatatttaa gaataaaatg atactgtttt
    11821 ccttaactta atgacattgg agtatactct agaggcccac ataataattg tgacataata
    11881 aagaaaagaa tgagacaagt aagtggaaca tttttatggt gctttttcat tccctcattg
    11941 tggttaactt cattttacag tttgcgaatt tttattgata taatatataa aggtaagaat
    12001 ataaaaaata atgcaattag gagcaaaaat gaaattagaa aacctattca tagaaaaatt
    12061 acatggtatt tataattatg atgttgattt taatgaagat attacttttt tatacggaag
    12121 taatggatgt gg
//
