LOCUS       BGC0000523             15996 bp    DNA     linear   BCT 14-JUL-2016
DEFINITION  Lactobacillus sakei ORF78 (partial), ORF116, ORF130 (partial), ORF84
            (partial), tnp1519L gene, tnp1516delC gene, tnp1516delN gene
            (partial), lasY gene, lasX gene, lasA gene, lasM gene, lasN gene,
            lasT gene, lasU gene, lasV gene, lasP gene, lasJ gene, lasW gene and
            tnp1519R gene.
ACCESSION   BGC0000523
VERSION     BGC0000523
KEYWORDS    .
SOURCE      Latilactobacillus sakei
  ORGANISM  Latilactobacillus sakei
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
            Latilactobacillus.
COMMENT     On Mar 11, 2001 this sequence version replaced Z54312.1.
            ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-07 19:45:55
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             <1..237
                     /codon_start=1
                     /db_xref="UniProtKB/TrEMBL:Q9AED5"
                     /note="ORF78"
                     /product="hypothetical protein"
                     /protein_id="CAC33833.1"
                     /transl_table=11
                     /translation="LPVLLKNGAPVDNSTLEVAKELFGSENIFDITIKNMERLKRYDVT
                     GVMFKDQFDHAVQNVYEKVTTEMISRIGAIENG"
     source          1..15996
                     /country="Norway"
                     /db_xref="taxon:1599"
                     /mol_type="genomic DNA"
                     /organism="Latilactobacillus sakei"
                     /strain="L45"
     protocluster    1..15996
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[6884:9662]"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      6885..9662
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
     cand_cluster    1..15996
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(LANC_like and DUF4135)"
                     /kind="single"
                     /product="lanthipeptide-class-ii"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..15996
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000523"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..15996
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lanthipeptide-class-ii"
                     /region_number="1"
                     /rules="cds(LANC_like and DUF4135)"
                     /subregion_numbers="1"
                     /tool="antismash"
     regulatory      217..222
                     /note="ORF116"
                     /regulatory_class="ribosome_binding_site"
     CDS             230..580
                     /codon_start=1
                     /db_xref="UniProtKB/TrEMBL:Q9AED4"
                     /note="ORF116"
                     /product="hypothetical protein"
                     /protein_id="CAC33834.1"
                     /transl_table=11
                     /translation="MGSLLNSNRVKSNSLDRGKRIEPENSMSIKDLSNPGEQSKSSKIE
                     SVTYYANVRINNHIKNKAEALSIIGVAKSQKDAIDRALDSFVEQLSDDEKRSFKNQLAT
                     LEAKDVKMKSEK"
     CDS             complement(799..>1200)
                     /codon_start=1
                     /db_xref="UniProtKB/TrEMBL:Q9AED3"
                     /note="ORF130; N-terminally truncated gene product;coding
                     sequence interrupted by insertion sequence"
                     /product="hypothetical protein"
                     /protein_id="CAC33835.1"
                     /transl_table=11
                     /translation="FAIKYIANSNNPFSIEESNGNVINVYFYPLGIALCERGNHSQLSA
                     KLKGVGETSIERIMNIEHVYDHVNYDGIYFYHIFSENEETNPYRFKAQNRTEFLAGIIF
                     EIGRLLKNYPELFPEEIKFAINNKEKIKQ"
     misc_feature    <1195..>1461
                     /note="insertion sequence IS1517"
     CDS             complement(1209..>1460)
                     /codon_start=1
                     /db_xref="GOA:Q9AED2"
                     /db_xref="InterPro:IPR001584"
                     /db_xref="InterPro:IPR012337"
                     /db_xref="UniProtKB/TrEMBL:Q9AED2"
                     /note="ORF84; Putative, N-terminally truncated
                     transposase;coding sequence interrupted by insertion
                     sequence"
                     /product="hypothetical protein"
                     /protein_id="CAC33836.1"
                     /transl_table=11
                     /translation="VANYQALEQDYQIKVYFCHPYSPWERGSNEYFNRRLRWFFPKKTN
                     FSQVTTDEILAALELINQRPLKIHHQQTAIERFRACSD"
     repeat_region   1462..1484
                     /note="IS1519L left inverted repeat"
                     /rpt_type=INVERTED
     misc_feature    1462..2270
                     /note="insertion sequence IS1519L"
     regulatory      1524..1529
                     /gene="tnp1519L"
                     /regulatory_class="ribosome_binding_site"
     gene            1524..2216
                     /gene="tnp1519L"
     CDS             1536..2216
                     /codon_start=1
                     /db_xref="GOA:Q9AED1"
                     /db_xref="InterPro:IPR001584"
                     /db_xref="InterPro:IPR012337"
                     /db_xref="UniProtKB/TrEMBL:Q9AED1"
                     /gene="tnp1519L"
                     /product="putative IS1519L transposase"
                     /protein_id="CAC33837.1"
                     /transl_table=11
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYDKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRSNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
     repeat_region   complement(2248..2270)
                     /note="IS1519L right inverted repeat"
                     /rpt_type=INVERTED
     repeat_region   2744..2770
                     /note="IS1516 left inverted repeat"
                     /rpt_type=INVERTED
     misc_feature    2744..4217
                     /note="insertion sequence IS1516"
     regulatory      2883..2888
                     /regulatory_class="ribosome_binding_site"
     gene            2897..3352
                     /gene="tnp1516delC"
     CDS             2897..3352
                     /codon_start=1
                     /db_xref="UniProtKB/TrEMBL:Q9AED0"
                     /gene="tnp1516delC"
                     /gene_functions="other (smcogs) SMCOG1206:transposase
                     (Score: 57.1; E-value: 2.6e-17)"
                     /gene_kind="other"
                     /note="N-terminal part of IS1516 transposase. Coding
                     sequence probably interrupted by nonsense mutation in codon
                     152 of original sequence."
                     /product="hypothetical protein"
                     /protein_id="CAC33838.1"
                     /transl_table=11
                     /translation="MCHDTTKLLLGIADENLKITDGETGEDGVIRLTGHLDYTPKACPK
                     CGIINDQKIINYGWRNTTIRFSKVLSNTVILALKRRYFHCKECHGNFLAQTVAVPKHCT
                     ISNTSRKECLEKLGEPVSLKHIANEVSASDSFVGRQLMRAERDFQTN"
     gene            <3353..4168
                     /gene="tnp1516delN"
     CDS             <3353..4168
                     /codon_start=1
                     /db_xref="GOA:Q9AEC9"
                     /db_xref="InterPro:IPR002560"
                     /db_xref="UniProtKB/TrEMBL:Q9AEC9"
                     /gene="tnp1516delN"
                     /gene_functions="other (smcogs) SMCOG1206:transposase
                     (Score: 193; E-value: 1.4e-58)"
                     /gene_kind="other"
                     /note="C-terminal part of IS1516 transposase. Coding
                     sequence probably interrupted by nonsense mutation in codon
                     152 of original sequence."
                     /product="putative protein"
                     /protein_id="CAC33839.1"
                     /transl_table=11
                     /translation="HYLPEIILMDEVKSTKSATGAMSFEFMNGETHELIDLLPYRTINK
                     LEKYFYRFDQAARENVKIIVTDMNYTYPKLVQTVFPNAMVVMDKFHIINALNRAFNKTR
                     IKVMKKCTSSSREFHALKRYWKLLLVPTEQLNFEQFKKWTNFPYWMAATDVVHQLLSLD
                     SELEQTYQVLNSVRSAVQHQDWHNYNAAFWNNKTYSEEMKNTIKTLESHHDEIRNTFTT
                     HYSNGPLEGSNNKIKAIKRASFAYRSFWRFRTRVLYVFKIKTKRALITK"
     repeat_region   complement(4191..4217)
                     /note="IS1516 right inverted repeat"
                     /rpt_type=INVERTED
     CDS             complement(4573..5475)
                     /codon_start=1
                     /db_xref="GOA:Q9AEC8"
                     /db_xref="InterPro:IPR003439"
                     /db_xref="InterPro:IPR003593"
                     /db_xref="InterPro:IPR017871"
                     /db_xref="UniProtKB/TrEMBL:Q9AEC8"
                     /gene="lasY"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 179.2; E-value:
                     1.7e-54)"
                     /gene_kind="transport"
                     /product="hypothetical protein"
                     /protein_id="CAC33840.1"
                     /transl_table=11
                     /translation="MLEIIDLNKSFKNRKVISHENFSVNNGEILGLIGQNGAGKTTTFK
                     MILDLLKPDSGTILWNKKPLTQKEHNIIGFLPEDRGLYPNLTVEQQIVFFAELRGQSPK
                     VTKLKIQRWMDRFDVKGKPSDKVKLLSKGNQQKVQLISTLIHDPKFIILDEPFSGLDPV
                     NAGLLKKGIKDMKDAGATIIYSSHDMANVEQISDKIIMLKNGRIVLNGTADDIKNSYSK
                     NKIIIQSALTKNDLENINGVMSVKKVADKFHVEIKNDLIGNKIASQAISKGALIEFIKD
                     RSTLEEIFQIKTTEPKTNE"
     gene            complement(4573..5487)
                     /gene="lasY"
     regulatory      complement(5482..5487)
                     /gene="lasY"
                     /regulatory_class="ribosome_binding_site"
     CDS             complement(5546..6403)
                     /citation=[5]
                     /codon_start=1
                     /db_xref="GOA:Q9AEC7"
                     /db_xref="InterPro:IPR010057"
                     /db_xref="InterPro:IPR010982"
                     /db_xref="UniProtKB/TrEMBL:Q9AEC7"
                     /gene="lasX"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR01716"
                     /gene_kind="biosynthetic-additional"
                     /note="essential for normal lactocin S production"
                     /product="putative Rgg-type transcriptional regulator"
                     /protein_id="CAC33841.1"
                     /sec_met_domain="TIGR01716 (E-value: 1.4e-61, bitscore:
                     198.2, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRVGELVHLIRESKNIPAINVYNSVMSKNMFYKFEKGNFDTTSEK
                     FMKIMSRLNISLKEFEFLLSEKKCLEYDTEFLKLTNAFYSKDISALQQIHNETKLMYQR
                     TLEIRFNHLSALSQIYYDMCSHNTTDQTNTNIIRNYLLNVENWTYYEWTLFNNSLSIFN
                     IEDVQFLINKSLQSLSIYETHNNFGNENILVLGNIAAMYIKSYNFEKIPSLVNRMETIH
                     LSEIAVFERLIFSFFEGVSLYVLGTDTSLKKSLDVIKVMDNMHLVHLSKDHQELLNIVQ
                     KNIV"
     gene            complement(5546..6476)
                     /gene="lasX"
     repeat_region   6405..6411
                     /citation=[5]
                     /rpt_type=DIRECT
     regulatory      complement(6408..6413)
                     /gene="lasX"
                     /regulatory_class="ribosome_binding_site"
     repeat_region   6429..6435
                     /citation=[5]
                     /rpt_type=DIRECT
     regulatory      6437..6442
                     /citation=[3]
                     /gene="lasA"
                     /regulatory_class="minus_35_signal"
     gene            6437..6792
                     /gene="lasA"
     regulatory      complement(6446..6451)
                     /citation=[5]
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /gene="lasX"
                     /regulatory_class="minus_10_signal"
     repeat_region   6461..6467
                     /citation=[5]
                     /gene="lasA"
                     /rpt_type=DIRECT
     regulatory      6463..6468
                     /citation=[3]
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /gene="lasA"
                     /regulatory_class="minus_10_signal"
     regulatory      complement(6471..6476)
                     /citation=[5]
                     /gene="lasX"
                     /regulatory_class="minus_35_signal"
     regulatory      6572..6577
                     /gene="lasA"
                     /regulatory_class="ribosome_binding_site"
     sig_peptide     6586..6678
                     /citation=[2]
                     /citation=[3]
                     /gene="lasA"
     CDS             6586..6792
                     /citation=[2]
                     /citation=[3]
                     /codon_start=1
                     /db_xref="GOA:P23826"
                     /db_xref="UniProtKB/Swiss-Prot:P23826"
                     /gene="lasA"
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /product="pre-lactocin S"
                     /protein_id="CAA91109.1"
                     /transl_table=11
                     /translation="MKTEKKVLDELSLHASAKMGARDVESSMNADSTPVLASVAVSMEL
                     LPTASVLYSDVAGCFKYSAKHHC"
     CDS_motif       6586..6648
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence: MKTEKKVLDELSLHASAKMGA"
                     /locus_tag="lasA_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       6649..6792
                     /prepeptide="core"
                     /locus_tag="lasA_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="-7.30"
                     /molecular_weight="4808.4"
                     /monoisotopic_mass="4805.4"
                     /core_sequence="RDVESSMNADSTPVLASVAVSMELLPTASVLYSDVAGCFKYSA
                     KHHC"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MKTEKKVLDELSLHASAKMGA"
                     /leader_location="[6585:6648](+)"
                     /alternative_weights="4826.4"
                     /alternative_weights="4844.4"
                     /alternative_weights="4862.5"
                     /alternative_weights="4880.5"
                     /alternative_weights="4898.5"
                     /alternative_weights="4916.5"
                     /alternative_weights="4934.5"
                     /alternative_weights="4952.6"
     mat_peptide     6679..6789
                     /citation=[2]
                     /citation=[3]
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /gene="lasA"
                     /product="lactocin S"
     stem_loop       6811..6843
                     /citation=[3]
                     /note="probably involved in modulating expression of
                     downstream genes transcribed from the lasA promoter"
     regulatory      6871..6876
                     /gene="lasM"
                     /regulatory_class="ribosome_binding_site"
     gene            order(6871..6876,6885..9662)
                     /gene="lasM"
     CDS             6885..9662
                     /citation=[1]
                     /citation=[3]
                     /codon_start=1
                     /db_xref="InterPro:IPR007822"
                     /db_xref="InterPro:IPR017146"
                     /db_xref="UniProtKB/TrEMBL:Q48849"
                     /gene="lasM"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: DUF4135"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: LANC_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1070:lanthionine synthetase C family protein (Score:
                     383; E-value: 4.8e-116)"
                     /gene_kind="biosynthetic"
                     /note="essential for lactocin S production; probably
                     involved in post-translational peptide modification"
                     /product="hypothetical protein"
                     /protein_id="CAA91110.1"
                     /sec_met_domain="LANC_like (E-value: 1.2e-24, bitscore:
                     77.0, seeds: 47, tool: rule-based-clusters)"
                     /sec_met_domain="DUF4135 (E-value: 2.5e-62, bitscore:
                     201.5, seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNEFEKKINELCFHYTLNLIFKDNLINLKKELNKFNNKNEIKMNL
                     YFSISQSIIYPFTDILLDLYHTELKTNSMLGNNSSERLKSFVSEFEQDHWEKINLTYPV
                     FFSTSELIVNDTTEYVSKILSNLINDNAELLSKFKIDYQKLLDIRLGKGDTHSGKSVAK
                     LMFRSPDEKKFDELLYKPRSNKTDITLKRCTDFISEGLNINFKFPKSLCNRNYSWYENI
                     EHVTTDDQVSINHYYYRSGCLLGLFWILGTTDIHSENIITNAGYPIVIDIETLSKGLSD
                     SEDKDVNFRLITSSVLSSCLLPVGKTFKGDSNFDFNGLFPKAQIGETAFEYRIVTDQDL
                     DWAYEMYPASIKNQKSKVTTNYIYPEEAFRPLKSGFFDTLSFIQKNSTYFLKIIKNNYV
                     SRQLLRDTQVYADFVHALQLPDNLKSVQNQQHILNILKDEFKPSKFGLSRLKNEVQSIS
                     KWDIPSFYTTNTSKNLLNFNGNTVIKNFWALSPEEAITKRVNSINLKSIKYQLRIIDLS
                     LATFYKSDDLFNVSNNNNNPVDIPIFTQQIKEEYFNQLDAIFNPSFGTSKIEDLYTLEF
                     GNDVTITTLQPGLYNSVGFLLSLWRYTQTKNVTEEERISIQRHVIFMYSQLVSPLKRYK
                     DLNIDDINVSVFSGLGGLLYCSCYLYLSTNEFEYVKDIKLLVQIICSRVLNTEIEELNF
                     EYLNGISGLLLLFQNLYKNVKLSNIVGGPFKKIIAHLSNIPFNQIINEDDVGIGHGISG
                     KLIGMSVLKLHGVNISNDVEKKCLSKVLNSISRSLEFNSSWCRGTLGILDMLLINNKLN
                     NSLYTSSMTNKFLKKYSYGINRSLECQKNISMCHGLGGIINFITSVNETKHNEIVPENK
                     FFSKLPNLRDTRVIRSSDFHPVSYMLSTTGLVDSILRYKNNMPNMVHLDIPNNV"
     regulatory      9662..9667
                     /citation=[3]
                     /gene="lasN"
                     /regulatory_class="ribosome_binding_site"
     gene            order(9662..9667,9677..10396)
                     /gene="lasN"
     CDS             9677..10396
                     /citation=[3]
                     /codon_start=1
                     /db_xref="InterPro:IPR005025"
                     /db_xref="UniProtKB/TrEMBL:Q48850"
                     /gene="lasN"
                     /note="essential for lactocin S production; probably
                     involved in post-translational peptide modification"
                     /product="hypothetical protein"
                     /protein_id="CAA91111.1"
                     /transl_table=11
                     /translation="MKIFAFSGAHHIGGLTNTIIERLVSGLKSGNDSLFFRNPTELSIK
                     YVESTEMFTSGIDTINDDIQMIRDEIASSDVIILGTPVYIRHISGSMKTFLDRLGAWTH
                     TYGLAGKRAIVFAVSSSTGEDTACEYLSYVSQQMGLAVDSELLINTSYQTTEDIFDQVD
                     FSIDKLKRHIADKEYIITDMQRKKFALSQTQLRNNQLFPHEIDVLSEKKLLEFGDFDAF
                     FRHMDNINREQSHYGDD"
     regulatory      10385..10390
                     /citation=[3]
                     /gene="lasT"
                     /regulatory_class="ribosome_binding_site"
     gene            10385..10390
                     /gene="lasT"
     gene            10399..12006
                     /gene="lasT"
     CDS             10399..12006
                     /citation=[3]
                     /codon_start=1
                     /db_xref="GOA:Q48851"
                     /db_xref="InterPro:IPR001140"
                     /db_xref="InterPro:IPR003439"
                     /db_xref="InterPro:IPR003593"
                     /db_xref="InterPro:IPR011527"
                     /db_xref="InterPro:IPR017940"
                     /db_xref="UniProtKB/TrEMBL:Q48851"
                     /gene="lasT"
                     /gene_functions="transport (smcogs) SMCOG1288:ABC
                     transporter related protein (Score: 123.8; E-value:
                     1.4e-37)"
                     /gene_kind="transport"
                     /note="probable exporter of mature lactocin S; essential
                     for lactocin S production"
                     /product="ABC transporter"
                     /protein_id="CAA91112.1"
                     /transl_table=11
                     /translation="MHIQNLFGRKKLILIIFFLTILAVSVNAIYPYITKIVIDNVLIGK
                     NVLLMRNIILGLGLIIIIQVLVDFFLSYLSSVWVQKNISDIRNLIFDRFLKIDPFSTNK
                     NGQTLIISDAEVIAANVQSILLTSLNSGLSIIVFGVIAFTISPLLTLVILSALPIFVLA
                     NIKLSSISKDKFFETQHSKDELLSKLTDFLSGKIFIKIYHAELYAKSELVNLNKQLKNN
                     SISLSTVMIMVKDVLAILTSVTPFIVLSIGMGLSLNHQISTGGLIAIYTYAGMIFSPVS
                     NLVNLMPAYQQIIVALERINNYLVLEPEGAAITTKLHDNSNKKVTLVNNINLVKNDVPI
                     LKNMTLNMDNEHIYMLTGPNGSGKSMLGKILTGLIFPSSGNIYNKNINTCLYVPADNYL
                     FDGSLKFNLTIGLDSYSNCELNTLVTKLNLVGNKLSFNSRLDNSKHTLSTGQIQKIKFI
                     RAILEHPDFLVLDEILENMDLNSRNIAIEIIKENSDETIFLVISHNLFTNLLTNKTKLI
                     EIQNSESGSVIRKEGRII"
     regulatory      11989..11994
                     /citation=[3]
                     /gene="lasU"
                     /regulatory_class="ribosome_binding_site"
     gene            11989..11994
                     /gene="lasU"
     gene            12003..12284
                     /gene="lasU"
     CDS             12003..12284
                     /citation=[3]
                     /codon_start=1
                     /db_xref="UniProtKB/TrEMBL:Q48852"
                     /gene="lasU"
                     /product="hypothetical protein"
                     /protein_id="CAA91113.1"
                     /transl_table=11
                     /translation="MRRTIAILPTFILITVFMYSLYLKAIHGIWINMFVFSLAGLGVYT
                     PIILFIDSLTLAFRGEKGNDIRSKLFYSYFIIILVAIILLSLYLMTHN"
     regulatory      12284..12289
                     /citation=[3]
                     /gene="lasV"
                     /regulatory_class="ribosome_binding_site"
     gene            order(12284..12289,12297..12674)
                     /gene="lasV"
     CDS             12297..12674
                     /citation=[3]
                     /codon_start=1
                     /db_xref="InterPro:IPR007556"
                     /db_xref="UniProtKB/TrEMBL:Q48853"
                     /gene="lasV"
                     /product="hypothetical protein"
                     /protein_id="CAA91114.1"
                     /transl_table=11
                     /translation="MEEQDFIKRQIKSFAQGIGSIINVGHANNGVEIMLPAEQGQNLPF
                     QDTLLRYLNMKHYKEGFIHLLKLESKMSQFDFNQLGIWYVYELNKIPSQLLIKDGLTEN
                     DISTLIHRLNQTPSDNKEMGK"
     regulatory      12659..12664
                     /citation=[3]
                     /gene="lasP"
                     /regulatory_class="ribosome_binding_site"
     gene            12659..12664
                     /gene="lasP"
     gene            12671..13471
                     /gene="lasP"
     CDS             12671..13471
                     /citation=[3]
                     /codon_start=1
                     /db_xref="GOA:Q48854"
                     /db_xref="InterPro:IPR000209"
                     /db_xref="InterPro:IPR015500"
                     /db_xref="UniProtKB/TrEMBL:Q48854"
                     /gene="lasP"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075:alkaline serine protease, subtilase family
                     (Score: 63.4; E-value: 3.8e-19)"
                     /gene_kind="biosynthetic-additional"
                     /note="probably involved in pre-lactocin S processing"
                     /product="serine protease"
                     /protein_id="CAA91115.1"
                     /sec_met_domain="Peptidase_S8 (E-value: 3.3e-15, bitscore:
                     46.3, seeds: 43, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTVLLRDINSAILTEYRLHMYSSRYTSSIALLDENINTTHSYLQK
                     HFSSISGHNNTASIHGTAMAGVLSIASLSKQKLTVFNTLDKYHQRTRKSVISALKMAII
                     NNFKLIIFPMSIILPKASNSTQKWVSDFNDVVALAKLNNATILSSAGNDGLNLDTLHPD
                     YIALPQMISSIITIGALGTDWRIASYSNFGKCIDYFFPGGETTSEIITTIGTDAKNIDF
                     QFIENVPRGFIKMIGTSLATGLFAGYALTVGIGSLQRFKSANFY"
     regulatory      13480..13485
                     /citation=[3]
                     /gene="lasJ"
                     /regulatory_class="ribosome_binding_site"
     gene            13480..13664
                     /gene="lasJ"
     CDS             13491..13664
                     /citation=[3]
                     /codon_start=1
                     /db_xref="UniProtKB/TrEMBL:Q48855"
                     /gene="lasJ"
                     /note="probably involved in lactocin S immunity"
                     /product="hypothetical protein"
                     /protein_id="CAA91116.1"
                     /transl_table=11
                     /translation="MKYSNKSVMLTMLFVSVLGTVMSFSFSGMLKAYELILATFFLIVA
                     VIYFFKFRNEKK"
     regulatory      13689..13694
                     /citation=[3]
                     /gene="lasW"
                     /regulatory_class="ribosome_binding_site"
     gene            13689..14946
                     /gene="lasW"
     CDS             13702..14946
                     /citation=[3]
                     /codon_start=1
                     /db_xref="UniProtKB/TrEMBL:Q48856"
                     /gene="lasW"
                     /product="hypothetical protein"
                     /protein_id="CAA91117.1"
                     /transl_table=11
                     /translation="MRTLVIIIKQVYKKKIFSLGYLLMVFSPVVLVAMGILVFSQINTN
                     KNVPSIAIISNNKVTQQIIYKSSKESTFHINNKLRTNGQAQAALKHNKIKGIFDVRINS
                     NATTVNFTGNRKNLKDQWSLITSFLNRINYSTRINESPLNATYKESLYKSIKINVYSKN
                     DTNYLSQRSNRISFITNILCIFFSMLLIFYCQMISQEVVSEKGTHILEIILSSITPTTH
                     FLGKISGVILLLLTQVIFYILLSPILYFTLNKTSLLGNSELIQNTHIFSLFISKPVIYT
                     IIFGFLGIYIYIVVSAVIASAVSRQEQVGEAITPVLMLALVSYYGGVLVMSHNTLHFLT
                     VSSYIPLISQNVMPIRFASATASNSEAIISIMISLVFSIILTHFAIYIYKRTVFIYNDN
                     GILQTFKTGLLKMKY"
     regulatory      14956..14987
                     /citation=[3]
                     /note="putative terminator of transcription initiated from
                     lasA promoter"
                     /regulatory_class="terminator"
     misc_feature    14999..15808
                     /note="insertion sequence IS1519R"
     repeat_region   15000..15022
                     /citation=[5]
                     /note="IS1519R left inverted repeat"
                     /rpt_type=INVERTED
     regulatory      15059..15064
                     /gene="tnp1519R"
                     /regulatory_class="ribosome_binding_site"
     gene            15059..15754
                     /gene="tnp1519R"
     CDS             15068..15754
                     /codon_start=1
                     /db_xref="GOA:Q9AEC6"
                     /db_xref="InterPro:IPR001584"
                     /db_xref="InterPro:IPR012337"
                     /db_xref="UniProtKB/TrEMBL:Q9AEC6"
                     /gene="tnp1519R"
                     /product="putative IS1519R transposase"
                     /protein_id="CAC33842.1"
                     /transl_table=11
                     /translation="MTMSHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILCDRGINVS
                     HTTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPPITSAFKKLKEYGFYQGTEHRTIKY
                     LNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKV
                     LLGIPA"
     repeat_region   complement(15786..15808)
                     /note="IS1519R right inverted repeat"
                     /rpt_type=INVERTED
ORIGIN
        1 cttccagtcc ttttaaaaaa tggggctcca gttgacaaca gtactttaga ggttgcaaaa
       61 gagttatttg gtagtgaaaa tatttttgat attacaatta aaaatatgga acggcttaaa
      121 cggtatgatg tgactggtgt aatgtttaaa gatcagtttg atcatgcagt acagaatgta
      181 tatgaaaaag ttacgactga aatgattagt agaatagggg ctattgaaaa tgggtagttt
      241 attaaattca aatagagtaa aatcaaatag tttagaccgt ggaaagcgta ttgaaccaga
      301 gaactctatg agcataaaag atttaagtaa tccaggcgaa caaagtaaga gcagtaagat
      361 tgagagcgtg acttattacg cgaatgtaag aattaataac catataaaaa ataaagcgga
      421 ggctctttca atcattggtg ttgccaagtc acaaaaggac gcaattgatc gtgctttgga
      481 ttcttttgta gagcagttaa gcgatgatga gaaaagaagt tttaaaaatc aattagcaac
      541 gctggaagca aaagatgtaa aaatgaaatc tgaaaagtaa aaaaatacta atgtatacat
      601 tagtattttt ttgtagatat actgatgaat aaacacctag tatctgttca catgtaaaaa
      661 tgtatataat tttatatgtg agtcgcttga ttagtatgga agtgcaataa acttaaacaa
      721 aaaaagcaag gtgtttgtac accctgctaa aagtagaaat attataattt cctgtttggc
      781 tgctaaatag cagtattttt attgctttat tttttcttta ttgttgatag caaattttat
      841 ttcttcaggg aaaagctcgg gatagttctt tagaagtcta ccaatttcaa atataattcc
      901 cgctaaaaac tcagttctat tttgggcttt aaatctatat ggattagttt cttcattctc
      961 ggaaaatatg tggtaaaagt aaataccatc ataattcaca tgatcgtaca cgtgttcaat
     1021 attcataata cgctcgattg aagtctctcc cacaccttta agtttagctg atagttgtga
     1081 gtgatttcct ctttcacata aagcaatacc tagtggatag aaatatacat ttataacatt
     1141 gccattactt tcttcgattg aaaaaggatt atttgaattg gcaatatatt taatggcaaa
     1201 ttacaagttt aatccgaaca agcccggaat ctttcaatgg cagtctgttg atgatgtatt
     1261 tttaatggtc gttgattaat tagttcaagt gctgctagga tctcatcagt cgttacttgg
     1321 ctaaaattgg tctttttcgg gaagaaccag cgtaaccgtc tattaaaata ttcattggaa
     1381 cctcgctccc atggtgaata tggatggcaa aaataaactt tgatctgata atcctgttct
     1441 aaggcctgat aattggcaac aggttctgtt gcaaagtttt aaatctacta tcaaataagg
     1501 taggataata gaaaaagata gcaggaggaa tgacgatgaa tcattttaaa ggaaagcaat
     1561 ttcagcagga tgtgattatt gtagccgtag gctactatct tcgttataac cttagctatc
     1621 gtgaagttca agaaatctta tatgatcgtg gcattaacgt ttctcatacg acgatttatc
     1681 gttgggtgca agaatatgac aaactactct atcaaatttg gaaaaagaaa aataaaaaat
     1741 ccttttattc atggaaaatg gatgaaacgt acatcaaaat taaaggaaaa tggcattatt
     1801 tgtatcgagc catcgatgca gatggtttaa ccttggatat ttggttacgt aaaaaacggg
     1861 acacacaagc agcctatgct tttcttaagc ggttagtgaa gcagtttgat gaaccgaagg
     1921 ttgtagtcac agataaagcc ccttctatta caagtgcctt taagaaacta aaagaatacg
     1981 gcttttatca agggacagaa catcgtacca ttaaatacct gaataatttg attgaacaag
     2041 accatcgtcc agtaaagaga agcaataaat tctatcgaag tttacgcact gcctctacca
     2101 cgattaaagg catggaagcc atccgaggat tatataagaa aacccgaaaa gaaggcactc
     2161 tcttcgggtt ttcggtctgt actgaaatca aggtattatt gggaatccca gcttaaatca
     2221 tagatatcgt aagggatttt attctttatt taaaactttg caacagaacc agttaatgaa
     2281 agaacagcta aagaaaaaag atgaacagct agcaacaaaa gacgaacagc tagcaacaaa
     2341 agacgagcaa ttaagcaagg tttataagtt gctcgatcaa cagcaacaat tagagctagg
     2401 aacgcaaaag agattaaaaa cagtgggaac agggaacgat ttgagcaaga ataaagcaga
     2461 aaatgtagag gaagagttaa ggaaggagca taaaacggct gaaaagcccc aaaaacggca
     2521 ctggcggcaa agaagacaat agagcagtgc ctatccaaac tgcaatagtt gggaagtgtg
     2581 ttaactgttt agctcgtttt ttgctccgtt tgtagtgctt ggtttactga ttgactagat
     2641 cagagacacc aaaagtaaaa tagacaacat ctagttcgct aaaaaagccc ctaacgattt
     2701 gtggtcgtta ggggctttgg tgtgcttact gtttactcat ttttttggtt ctacaatatc
     2761 tgttgttggt aatagatttt catctattac tgatgacaga ttttttattt atggctagaa
     2821 taaaaaagcg gacatttcct gtccgttcat ccaagtcaac caccacgaag atttttcaga
     2881 aagaaggaaa tgccctatgt gtcatgatac tacaaaatta ttattaggaa tagctgacga
     2941 gaacctaaaa attactgacg gtgaaactgg agaagacggg gttattcgct taactggcca
     3001 cttggattac acgccgaagg cttgtcccaa gtgcgggatt atcaatgatc aaaagattat
     3061 taactacggc tggcgcaata ccactatcag attctcgaag gttctgagca acaccgtcat
     3121 cttagcctta aagcgccgct atttccactg taaagaatgt cacggcaact ttcttgcaca
     3181 aacagtggca gttccgaaac actgtacaat ttcaaacacc agccgcaaag aatgcctaga
     3241 gaagctcggt gaaccggtct ctttgaagca cattgccaat gaagtatcgg catcagattc
     3301 atttgtgggc cgccagttaa tgcgcgctga acgagacttt caaaccaatt agcactattt
     3361 accagagatc atcttaatgg atgaggtgaa gagtaccaag agcgctactg gtgccatgag
     3421 ctttgagttc atgaatggcg aaacccatga attaatcgac ttactccctt atcgaaccat
     3481 caataaacta gagaagtatt tttaccgctt cgatcaagct gcccgagaaa acgtcaaaat
     3541 tattgtgact gatatgaatt atacctaccc caagcttgtg cagaccgtgt tccccaatgc
     3601 catggtggtc atggacaagt ttcatattat caacgcccta aatcgggcgt ttaacaaaac
     3661 cagaatcaag gtgatgaaga agtgtacctc gtcttctcgc gaatttcacg cccttaagcg
     3721 ctactggaag ctgttgttag taccaactga acagcttaac tttgaacaat tcaaaaagtg
     3781 gactaatttt ccttactgga tggctgccac cgatgttgtc catcagctgt tatcgttgga
     3841 ttcagaacta gaacaaactt atcaagtctt aaacagcgtc cggtcagctg tccaacatca
     3901 agattggcat aactacaatg cggcattttg gaataacaaa acgtattctg aagaaatgaa
     3961 gaacacgatt aaaacacttg aaagccatca tgatgagatt cgtaacacct ttaccacaca
     4021 ctattcaaat ggtcctttgg aaggttctaa taataaaatc aaggcgatca aacgagccag
     4081 ttttgcgtat cgcagcttct ggaggtttag aactcgagta ctatacgttt tcaaaatcaa
     4141 aacaaaaaga gccctaatca cgaagtgatt agaactcgaa atatggattc accaacaaca
     4201 gttgacgaag agccaaatca aagaacaatt ttcgctaatc tcttctattt tgaaggagtt
     4261 atggcacagc ccgttctttt catatttctg tcgctattta aatttttcaa gttgtatagc
     4321 taagtattgt tcaattaatt atggcacaaa tgtctaaata tttatgtatc aaatactagg
     4381 ataggagcag cacaattcca cttgtcaacg aaaaaatgca aaccaaacat taaaactatc
     4441 tttttcaatg tataagcagc attaggtttt aaatatacac attcgcacct gttcaaatat
     4501 tagcaaactt ttcttgacat atctttcaat gcctttttat tttgattctg taaaaagtta
     4561 aaacgctaac ttttattcat ttgtttttgg ctcagttgtt ttaatttgaa atatttcttc
     4621 caaagtagag cggtctttta taaactctat gagtgccccc ttgctgatag cctgagaagc
     4681 tattttgttg ccaatcaaat catttttaat ttctacatga aatttgtcag caactttttt
     4741 tacagacata acaccgttga tgttctctaa atcatttttt gtgagagcag attgtataat
     4801 aatcttattt ttagagtagc tattcttaat gtcatcagca gtaccattaa gaactatacg
     4861 tccattttta agcatgataa ttttgtcact aatttgttct acgtttgcca tatcatgaga
     4921 tgaataaata attgttgctc cagcatcttt catatcctta atgcccttct taagaagacc
     4981 tgcatttact ggatctaaac cactaaatgg ctcatcaagt attataaact ttggatcatg
     5041 aattaatgta gatattagtt gaactttttg ctgattacct tttgagagta acttgacttt
     5101 gtcagaaggc ttccccttaa cgtcgaatct gtccatccaa cgttgtattt ttaatttggt
     5161 taccttaggt gattgtccac gcaactcggc aaaaaaaaca atttgctgtt caacagtcaa
     5221 attaggatat agtcctctat cctctggtaa aaatccgata atattatgtt ctttttgggt
     5281 aagaggcttt ttattccata aaatggttcc tgaatctggt ttaagcaaat ctaaaatcat
     5341 cttgaacgtt gtagtttttc ctgcaccgtt ttgacctatc agtcccaata tttcaccgtt
     5401 attaacacta aaattttcgt gggaaattac ttttctattt ttgaagcttt tatttaaatc
     5461 aataatttct aacaagattt caccttcctc cacactagat tagcacagaa gatttgaaat
     5521 taaaacaaca tactacattc atattttaaa caatattttt ttgaacaatg tttaaaagct
     5581 cttgatggtc ttttgataaa tgaactaaat gcatattatc cattactttt ataacatcca
     5641 aacttttctt taaagaagta tctgttccta aaacgtatag tgacactccc tcaaaaaatg
     5701 aaaatatcaa tctttcaaaa acagctattt cagaaagatg tattgtttcc attcgattta
     5761 ctaaagaggg aattttttca aagttataac tttttatata cattgcagca atatttccta
     5821 aaaccaaaat attttcgtta ccaaaattgt tatgtgtttc atatatcgaa agactttgta
     5881 aggacttatt tataagaaat tgtacatctt ctatattaaa aatggataaa gagttattaa
     5941 ataaagtcca ttcataataa gtccaatttt cgacattcaa tagataattc ctaattatat
     6001 tggtgtttgt ttgatcagta gtattatggg aacacatatc atagtaaatt tgtgatagtg
     6061 ctgataaatg gttgaaccta atctctagtg ttctttgata cattaatttt gtttcattat
     6121 gaatttgttg taaagcagat atatcttttg aataaaatgc gtttgttaat tttaaaaact
     6181 cagtatcata ctctaagcat ttcttttcac ttaataaaaa ttcaaattct ttcaaactaa
     6241 tatttagtct agacattatt ttcatgaatt tttcagatgt tgtatcaaag tttccctttt
     6301 caaacttata aaacatattt tttgacatca cactattata cacatttatc gcaggtatat
     6361 ttttagattc tctaataaga tgtactagtt ctcctactct cattttatcc cccctaaaat
     6421 ttaatatatt atccctttgc actcaatatt aacatataaa tttaccctta agtcataaat
     6481 gattgatcga aagggtgagt ctatgtatct tattgggtta tttctattat aataataaaa
     6541 aagtttattt aaaaaaaaca aggcacggaa gggaggtgga acagtatgaa aacagaaaaa
     6601 aaggttttag atgaactgag cttacacgct tctgcaaaaa tgggagcacg tgatgttgaa
     6661 tccagcatga atgcagactc aacaccagtt ttagcatcag tcgctgtatc catggaatta
     6721 ttgccaactg cgtctgttct ttattcggat gttgcaggtt gcttcaaata ttctgcaaaa
     6781 catcattgtt agatagattg cttttattga agctggtaaa tcatcttctg gtgatatcca
     6841 gcttttttaa tttatcaata gcttgaagca ggaggtaatg ctacttgaat gaatttgaaa
     6901 aaaaaataaa tgaattgtgt tttcattaca cattaaacct tatatttaaa gacaatttaa
     6961 taaacctaaa aaaagaatta aacaaattta acaataaaaa cgagattaag atgaatttat
     7021 atttctccat ctcgcaaagt ataatatatc ctttcacaga catactgcta gatctatacc
     7081 atacggaatt aaaaacaaat agcatgcttg gaaataatag ctctgagaga ttaaaaagtt
     7141 tcgtttctga atttgaacaa gatcattggg aaaaaataaa tttaacctat ccagttttct
     7201 tctccacatc tgaactaatc gttaatgata caacggaata tgtttcaaaa atattgagca
     7261 acttaataaa tgataatgct gaattattga gtaaatttaa aattgattac caaaaattac
     7321 tagatattag actaggcaaa ggggacactc attctggtaa aagtgtcgcc aaattgatgt
     7381 ttagatcccc tgatgaaaaa aaatttgatg aactactata caaacccaga agtaataaaa
     7441 cagatataac tcttaaaaga tgtacagact ttatttctga gggtctaaat attaatttta
     7501 agtttcctaa atccctatgt aaccgtaatt atagttggta cgaaaatatt gaacatgtta
     7561 ccactgatga tcaggtatca atcaatcatt attattatcg atcagggtgt ctacttggat
     7621 tattttggat attaggaacc acagatatac acagcgaaaa cattattact aatgcaggct
     7681 accctattgt tattgatata gaaaccttgt caaaaggtct gagtgatagt gaagataaag
     7741 atgtgaattt tcgattaatt acatccagcg tactttcgtc atgcttactt ccagtaggta
     7801 aaacatttaa aggtgatagt aactttgatt tcaatggact atttcctaaa gcacaaattg
     7861 gagaaactgc atttgaatat agaatagtca cagaccaaga tctagactgg gcatatgaaa
     7921 tgtatccagc tagtatcaaa aatcaaaaat ctaaagtaac aactaattat atttaccccg
     7981 aagaagcctt caggccactc aaatccggtt ttttcgatac attatctttt attcaaaaaa
     8041 attcaactta ttttttaaaa attattaaaa acaactatgt atcaagacag cttctcagag
     8101 atacgcaggt gtatgcagat tttgttcatg ctttgcagtt accagacaat cttaagtcag
     8161 ttcaaaatca acagcatatt ttaaatattt taaaagacga atttaagcct tctaaatttg
     8221 gattatcaag attaaagaac gaagtacaat ctatatctaa gtgggatatc ccatcgtttt
     8281 atactaccaa tacaagtaaa aatctattga attttaatgg gaatactgtc attaaaaact
     8341 tttgggcact ttctcctgaa gaagccatta caaaaagggt aaattcaata aatttaaaaa
     8401 gcattaaata ccagctaaga ataatcgacc tatcacttgc aacattttat aagagcgatg
     8461 atctcttcaa cgttagcaat aataataata acccagttga tattccaata ttcactcaac
     8521 aaattaaaga agaatatttt aaccaattag atgctatatt caatccatcg tttggtacca
     8581 gtaaaatcga agacctgtat actctagaat tcggaaatga tgtaacaatt accacgttac
     8641 agccgggttt atacaactca gtgggatttc tacttagctt atggcgatat acacaaacca
     8701 aaaatgtaac ggaagaagaa agaatttcta ttcaaagaca tgtaattttt atgtatagtc
     8761 aattagttag tcctttaaaa aggtataaag atttaaatat tgacgatatt aatgtatcgg
     8821 tattttctgg actaggtgga ttactatatt gtagctgcta tttgtattta tctacaaatg
     8881 agtttgaata cgtcaaagac attaagctgt tagttcaaat tatttgcagt agagtcctaa
     8941 acacagaaat cgaagaactt aattttgaat atttgaatgg gatttcagga ttgttactat
     9001 tatttcagaa cttatacaag aatgttaaac tatctaatat tgtaggtggt ccttttaaaa
     9061 aaataatagc acatttatca aatatcccat tcaatcaaat tattaatgaa gacgatgttg
     9121 gtataggaca tgggatttca ggaaagttaa ttggtatgtc tgtattaaaa ttgcatggtg
     9181 taaacataag taacgatgta gaaaaaaaat gcctaagtaa agtacttaac tcaattagtc
     9241 gtagtttaga gtttaatagc agctggtgca gaggaacact cggtatttta gatatgctgc
     9301 ttataaataa caaattaaac aattcgcttt atacatcttc aatgactaat aagtttttaa
     9361 aaaaatatag ctatggaata aataggtctc tcgaatgcca gaaaaatatt agtatgtgcc
     9421 atggtctagg aggtatcatt aactttatta cctctgttaa tgagaccaaa cataatgaaa
     9481 ttgtacctga gaataaattt ttttcaaaat taccaaattt aagggatact agagtgatta
     9541 gatccagtga ttttcatcca gtttcatata tgttatccac tacaggattg gtggactcca
     9601 ttttgcgata caaaaataat atgccaaaca tggttcatct cgatatacca aataatgttt
     9661 aggaagtgat tgagttatga aaatctttgc cttttcaggc gctcaccata ttggagggct
     9721 cacaaataca ataatagaga gactagtaag tggtttaaaa agtggtaatg atagtttgtt
     9781 cttcagaaac cctacagagt tatctatcaa atatgtagag agcacagaaa tgtttaccag
     9841 tggtattgac acaattaacg atgatattca aatgataaga gacgaaatag caagttcaga
     9901 tgttatcatc ttaggaacac cggtttatat tagacatata tcaggtagta tgaaaacatt
     9961 cttagataga ttgggagcat ggacacatac ttatggatta gcggggaaaa gagcaattgt
    10021 atttgcagtt tctagttcta caggtgaaga taccgcgtgt gagtacctaa gctatgtttc
    10081 ccagcaaatg gggttagccg tagattctga attattaata aacacatcat atcaaactac
    10141 tgaagatata tttgatcaag ttgatttttc tattgacaaa ttaaaaagac atatagcgga
    10201 taaggaatat attataacgg atatgcagag aaaaaaattt gccttatcac aaacccagct
    10261 tcgtaacaac cagcttttcc cacatgaaat tgatgtacta tctgaaaaaa aacttttaga
    10321 atttggtgat tttgatgcct tcttccgcca tatggacaat ataaatagag agcaaagcca
    10381 ctatggagac gactaagtat gcatattcaa aatttatttg gcagaaaaaa gttaatattg
    10441 attatctttt ttctaactat tttggctgta agtgttaatg caatttatcc atatataact
    10501 aagatcgtaa tagataatgt gttaataggt aaaaatgtgc tcctcatgag aaatataatt
    10561 ttgggactgg gcttaataat tatcattcaa gtccttgttg atttcttctt gagttatttg
    10621 tcctctgtat gggtacaaaa aaatattagt gacatcagaa acttaatttt tgatagattt
    10681 ttaaaaattg atcctttttc aacaaataag aacggtcaaa ctctaataat ttcagatgcg
    10741 gaagtcatag ctgctaatgt acaatcaata cttctaacaa gccttaacag cgggttgtct
    10801 ataatagttt ttggggtaat tgcatttact ataagtcctc tcctaacttt ggttatctta
    10861 tctgctctac cgatatttgt tcttgcaaat atcaaactaa gtagtatttc taaagacaaa
    10921 ttttttgaaa cacaacattc taaggatgaa ttattatcca aattaacaga tttcttgagt
    10981 ggaaagattt ttataaaaat ataccatgcc gaattatatg caaaaagtga attggtaaat
    11041 ttgaataagc aactaaaaaa taatagtata tctttaagta cagttatgat aatggttaag
    11101 gatgtattag caattttaac ttcagtaact ccatttattg ttttaagtat tggtatgggg
    11161 ctatctttga atcatcaaat ttccaccgga ggactcattg ctatttatac gtacgcgggt
    11221 atgatatttt ctccagtaag taacttagta aatttaatgc cggcgtatca acaaattata
    11281 gtcgcacttg agcgtattaa taattatttg gtactagaac cagaaggagc tgctataact
    11341 actaaattac atgataattc taataaaaaa gtaacattag ttaataatat aaacctggtt
    11401 aagaatgatg tacccatatt aaaaaatatg accttgaata tggataatga gcatatatat
    11461 atgctcacag gacctaatgg gtctggaaaa tctatgcttg gaaaaatatt aactggcctt
    11521 atttttccaa gttcgggtaa tatttataat aaaaacataa atacttgcct atatgtacct
    11581 gcagataatt acttgtttga tggctcctta aaatttaatt taacaatagg attagattca
    11641 tactctaatt gtgaattaaa cactcttgtt actaagttaa acttagtagg aaataagtta
    11701 tcatttaact ctagattaga taactcaaaa cataccctaa gtacaggaca aattcaaaag
    11761 ataaagttca tacgcgcaat actagaacat cctgattttt tagttttaga tgaaatatta
    11821 gaaaatatgg atttaaattc aagaaatata gccattgaaa taattaaaga aaactctgat
    11881 gaaactatat ttttagttat ctcacataac ttatttacaa atttactaac taataaaact
    11941 aaacttattg aaattcaaaa ttcagaaagt ggctctgtta tcaggaaaga gggtaggata
    12001 atttgagaag aactatagca atattaccaa catttatttt aataacagtc tttatgtata
    12061 gcctttattt aaaagctata catggtattt ggataaatat gtttgtattt agtctagcgg
    12121 gattaggagt atacactcct atcattctat ttattgatag tttaacattg gcatttagag
    12181 gagaaaaagg taacgacata agatctaaac ttttttattc atattttatt attatactag
    12241 tcgctatcat tttactttcg ttatatttaa tgacacataa ttaagaggta ttacaaatgg
    12301 aagaacaaga tttcataaaa cgacaaatca aatcttttgc acaaggaatt ggaagtatta
    12361 ttaatgtggg ccatgctaat aatggggtag aaattatgct tccagctgaa cagggtcaaa
    12421 atttaccatt ccaagatacc cttcttagat atttaaatat gaaacattac aaggaaggtt
    12481 ttatccatct actcaagtta gaaagcaaaa tgtctcagtt tgatttcaac caattaggta
    12541 tttggtatgt atatgaatta aataaaatac cgtctcagtt attaattaaa gatggcctaa
    12601 cagaaaacga tatatcaacg ctaatccata gattaaatca gaccccttcg gataataagg
    12661 aaatgggcaa atgaccgtgc ttttaagaga cataaattca gctattttaa cagaatatag
    12721 gctacacatg tacagctcga gatatacatc atcaattgct ttactggacg aaaatattaa
    12781 tacgacacac tcttatttgc agaagcattt tagtagtata tcaggtcata ataataccgc
    12841 gagtattcat ggaacagcta tggctggtgt acttagtata gcaagcttga gtaaacaaaa
    12901 attaacagtc tttaatactc tagataaata ccatcaaaga actcggaaaa gtgtgatatc
    12961 agcgctcaaa atggccataa taaacaattt taaattaatc atatttccta tgagtatcat
    13021 tttacccaaa gcaagtaata gcactcagaa atgggtatct gatttcaatg atgttgtagc
    13081 attagccaaa cttaataatg ctactatact cagctcagcg ggaaatgatg gcctaaatct
    13141 tgacacttta caccccgatt atattgcact acctcaaatg atttcaagca ttataacaat
    13201 tggtgcatta ggaacagatt ggagaattgc aagttattct aattttggaa aatgtataga
    13261 ttatttcttt cctggagggg aaaccacaag tgaaattatt acaactatag gaacagatgc
    13321 aaagaatatt gattttcaat ttattgagaa tgtacctaga ggatttataa aaatgattgg
    13381 gaccagtttg gccactggac tttttgcagg atatgcactt actgtgggaa taggatccct
    13441 tcagaggttt aaatctgcta acttttacta aaattaagga gaggtttatt atgaaatatt
    13501 caaataaatc agtaatgttg accatgttat ttgtttcagt gctaggaaca gttatgtctt
    13561 tttcattttc aggaatgtta aaagcatacg aattaatact cgctacattt tttttaatag
    13621 ttgctgtaat ttactttttc aaatttagaa atgaaaaaaa atgaagtttc acacatttta
    13681 tagaaagagt gggtagctta catgcgtacg ttagtgataa ttataaaaca ggtatacaaa
    13741 aaaaaaatct ttagtttagg ttacttgctg atggtttttt cccctgtagt gcttgttgct
    13801 atgggaattc tagtcttttc ccaaattaac accaataaaa acgtaccttc tattgctata
    13861 ataagtaata ataaggttac tcagcaaata atatataaat ccagtaagga aagtactttc
    13921 cacataaata acaagcttcg aaccaatggt caagcccaag cagcgttaaa acacaataaa
    13981 ataaagggta tctttgacgt taggattaat tccaatgcaa caactgttaa ttttactgga
    14041 aacaggaaaa acttaaaaga ccaatggtct ttaataacga gctttttaaa tagaataaat
    14101 tattctacca gaattaatga gagtccatta aatgccactt acaaagaatc attatataaa
    14161 tctattaaaa taaacgtata tagtaaaaat gacacaaact atttgtcaca aaggtcaaac
    14221 agaatttcat tcattacaaa tatcttatgt atattttttt cgatgctgtt gatattttac
    14281 tgtcaaatga tatcgcaaga agtagtttct gaaaaaggaa cacatatact agaaattatt
    14341 ttgtctagca ttactccaac cactcatttt ttaggtaaaa tttccggagt tattttgctg
    14401 ttattgacac aagttatttt ttatatcctt ttatctccaa tattatattt cacgttaaat
    14461 aaaacctctt tgctaggaaa ttcagaattg attcaaaata cccatatatt tagtcttttt
    14521 atatcaaaac ctgttatata taccattata tttggctttt tgggtatata tatatatatt
    14581 gtagtatccg ctgtaatagc ttctgccgtt tcccgacagg aacaggttgg agaggccatc
    14641 acacctgtat taatgctagc actagtgagt tattatggtg gagtcttagt aatgtcacat
    14701 aacacgctgc attttttaac agtatcttca tacattccct taatttctca gaacgtaatg
    14761 cccatcaggt tcgcaagtgc tactgcttct aactcagaag ccataatcag tataatgata
    14821 tcattggtat tttcaataat actaacgcat tttgccatat atatctataa acgaactgtt
    14881 ttcatttaca atgataatgg tatattacaa acctttaaaa caggacttct aaaaatgaaa
    14941 tactaataag tattctcagt agaagtggag ttcaccactt ctactgatgt ttctagaaag
    15001 gttctgttgc aaagttttaa atctactatc agataaggta gaataataga aaaagatagc
    15061 aggaggaatg acgatgagtc attttaaagg aaagcaattt cagcaggatg tgattattgt
    15121 agccgtgggc tactatcttc gttataacct tagctatcgt gaagttcaag aaatcttatg
    15181 tgatcgtggc attaacgttt ctcatacgac gatttatcgt tgggtgcaag aatatggcaa
    15241 actactctat caaatttgga aaaagaaaaa taaaaaatcc ttttattcat ggaaaatgga
    15301 tgaaacgtac atcaaaatta aaggaaaatg gcattatttg tatcgagcca tcgatgcaga
    15361 tggtttaacc ttggatattt ggttacgtaa aaaacgggac acacaagcag cctatgcttt
    15421 tcttaagcgg ttagtgaagc agtttgatga accgaaggtt gtagtcacag ataaagcccc
    15481 tcccattaca agtgccttta agaaactaaa agaatacggc ttttatcaag ggacagaaca
    15541 tcgtaccatt aaatacctga ataatttgat tgaacaagac catcgtccag taaagagacg
    15601 caataaattc tatcgaagtt tacgcactgc ctctaccacg attaaaggca tggaagccat
    15661 tcgaggatta tataagaaaa cccgaaaaga aggcactctc ttcgggtttt cggtctgtac
    15721 tgaaatcaag gtattattgg gaatcccagc ttaaatcata gataccgtaa gggattttat
    15781 tctttattta aaactttgca acagaaccag tctccaaaag tactatcttc atctgaatcc
    15841 cttgttccat taattaacct actactccaa aaagatgttt tcccgacaac taaaacatta
    15901 ttattttcta aaatacttat aaaaacaaat tgataatttt cttgtgcctt atcttttcct
    15961 tgagcatata cagcaccttt gtttttgaat tttagt
//
