LOCUS       BGC0000527             12324 bp    DNA     linear   BCT 17-MAR-2011
DEFINITION  Bacillus sp. HIL-Y85/54728 mersacidin biosynthesis gene cluster
            (mrsK2, mrsR2, mrsF, mrsG, mrsE, mrsA, mrsR1, mrsD, mrsM and mrsT
            genes).
ACCESSION   BGC0000527
VERSION     BGC0000527
KEYWORDS    .
SOURCE      Bacillus sp. HIL-Y85/54728
  ORGANISM  Bacillus sp. HIL-Y85/54728
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
COMMENT     On Mar 17, 2011 this sequence version replaced AJ250862.1.
            ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 13:47:23
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     gene            complement(1..1440)
                     /gene="mrsK2"
     CDS             complement(1..1440)
                     /codon_start=1
                     /db_xref="GOA:Q9RC29"
                     /db_xref="HSSP:1JOY"
                     /db_xref="InterPro:IPR003594"
                     /db_xref="InterPro:IPR003661"
                     /db_xref="InterPro:IPR004358"
                     /db_xref="InterPro:IPR005467"
                     /db_xref="InterPro:IPR009082"
                     /db_xref="UniProtKB/TrEMBL:Q9RC29"
                     /function="putative histidine-kinase"
                     /gene="mrsK2"
                     /gene_functions="regulatory (smcogs) SMCOG1003:sensor
                     histidine kinase (Score: 163.7; E-value: 1.5e-49)"
                     /gene_kind="regulatory"
                     /product="MrsK2 protein"
                     /protein_id="CAB60253.1"
                     /transl_table=11
                     /translation="MKLRNRIAFYFVSRLFLLMFIWGVLIVGSIIFFSLVLGQNKMETP
                     HLSLKKIVDQTVVADKQFSINTKAKEDLLENRMWIQILDEQGDEVFSFNKPQNIPKHYI
                     LGELISDYMYPAKKGYQLSTWYETKNNQDLTWVLGKPMEKNNPFFYWVNNLWIVSIIMA
                     GIVIAIFYGKKLGAPLLFIVSWIENLSKEKYEEPYDWKFHSSKYRHNKSGYKTYQELMQ
                     ALSELTSTLQRNKAERELLEKTREEWMAGVSHDLKTPLSVIKGYTVLLSSNEYEWENQQ
                     VRDFSLIMEERVEYMEKLIEDFNLTFQLKNESLPIHLEEKNVVKVIHDTLEKMKLLPES
                     ENKRFALESSHNKILFNMDERYLKRAVENLIANCIKHNPPGTKIKVILYQDVLHDNEIQ
                     IIIEDDGIGMDEETVECLFDRYFRGTSATSNNSGTGLGMAIAQQIIKAHGGNIDIYSQP
                     GIGTRFKITFPSPPSKYNNVN"
     source          1..12324
                     /db_xref="taxon:69002"
                     /mol_type="genomic DNA"
                     /organism="Bacillus sp. HIL-Y85/54728"
     protocluster    1..12324
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[6891:10080]"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      6892..10080
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
     cand_cluster    1..12324
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(LANC_like and DUF4135)"
                     /kind="single"
                     /product="lanthipeptide-class-ii"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..12324
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000527"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..12324
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lanthipeptide-class-ii"
                     /region_number="1"
                     /rules="cds(LANC_like and DUF4135)"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(1437..2159)
                     /gene="mrsR2"
     CDS             complement(1437..2159)
                     /codon_start=1
                     /db_xref="GOA:Q9RC28"
                     /db_xref="HSSP:1GXP"
                     /db_xref="InterPro:IPR001789"
                     /db_xref="InterPro:IPR001867"
                     /db_xref="InterPro:IPR011006"
                     /db_xref="InterPro:IPR011991"
                     /db_xref="UniProtKB/TrEMBL:Q9RC28"
                     /function="putative response-regulator"
                     /gene="mrsR2"
                     /gene_functions="regulatory (smcogs) SMCOG1008:response
                     regulator (Score: 177.2; E-value: 6.2e-54)"
                     /gene_kind="regulatory"
                     /product="MrsR2 protein"
                     /protein_id="CAB60254.1"
                     /transl_table=11
                     /translation="MTLEENKILMVDDDEHILNLLITCFEKEGFSNISTAMTGSETLLK
                     IDQELPNIILLDVMLPDTDGFTLCSKIRSHTNVPILFLTAKTTDLDKLQGFSFGGDDYI
                     TKPFNPLEIVARVKAQLKRTTQLSSSYPLKQVYDYEYFKIDPNTGRLVVNGTRIDCPAQ
                     EMKLLIYFCEHPNQILSKHQIYKDVWGEFYGGDSTVMVHVRRLREKLEEDPSRPRWIKT
                     VRGQGYIFEAEPIDEDLF"
     gene            2402..3313
                     /gene="mrsF"
     CDS             2402..3313
                     /codon_start=1
                     /db_xref="GOA:Q9RC27"
                     /db_xref="InterPro:IPR003439"
                     /db_xref="InterPro:IPR003593"
                     /db_xref="InterPro:IPR017871"
                     /db_xref="UniProtKB/TrEMBL:Q9RC27"
                     /function="putative ABC-transporter"
                     /gene="mrsF"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 184.7; E-value:
                     3.7e-56)"
                     /gene_kind="transport"
                     /product="MrsF protein"
                     /protein_id="CAB60255.1"
                     /transl_table=11
                     /translation="MNDQIVVTHDLTKKYKKHTSVDGLNLRIRRGEIYGFLGPNGAGKT
                     TTIRMLLGLIKPTKGNIEIFGQNLNKNRLQILQRIGSLVESPTYYGNLTGYENLEAVRR
                     LRGLPEQQVNEVLETVRLSKVANRLTKEYSLGMKQRLGIAVALLSSPDLLILDEPTNGL
                     DPSGIQEIRELIKELPKSGMSVIVSSHLLSEIDQMATQVGIINNGKMIFQDSIASLHQK
                     RKPLLKVGVSDVIEAKTILNRKGLKVDLQKNYLWLSQTEPEFVSEINSILLHSGLSVFR
                     LEEKTRSLEDIFLELTGTEGSL"
     gene            3310..4068
                     /gene="mrsG"
     CDS             3310..4068
                     /codon_start=1
                     /db_xref="UniProtKB/TrEMBL:Q9RC26"
                     /function="putative ABC-transporter integral membrane
                     protein"
                     /gene="mrsG"
                     /product="MrsG protein"
                     /protein_id="CAB60256.2"
                     /transl_table=11
                     /translation="MKKLLWADQLKLKRSSLLIVVLLVPLLIIAYELVNLTYRSEYVEK
                     QAEMFHAGSMWMYLLYDNSLLFGLGFPLAATLSASIIANIEHQANGWKQTLSFPVSRMR
                     IYVSKFICLVVSLFISSTIFLLGMVLLGKLVGFEGSVPWGLLFGDSYSMLVTVLPIMAF
                     QIWLSMVFSNQAFSILVGSVSSIMGLFLAAAQSTRWFPLAYPSQSSTVILQYEGIGYNP
                     DLSSYLCISLFLGIIILFLGSIHFAKRDAL"
     gene            4085..4819
                     /gene="mrsE"
     CDS             4085..4819
                     /codon_start=1
                     /db_xref="UniProtKB/TrEMBL:Q9RC25"
                     /function="putative ABC-transporter integral membrane
                     protein"
                     /gene="mrsE"
                     /product="MrsE protein"
                     /protein_id="CAB60257.1"
                     /transl_table=11
                     /translation="MKNILFVERLKLKRSKLWIIYLLGPLLGVSLAYTNFIKNYNLFMN
                     PGDNPWVEAWTQVALFMGPFVLPIVVGIFAALVCRGEHVGGGWKQLLALPVKHSDIFLG
                     KFLTVVRMIFISMSILILLFIGFGYMLGISGSLPLLTILGYGIRGILACLPLILLQLIV
                     SIRSKTFGIPLAVSIVFTLPAIIIASTPLGQVYPWTQPMLAMSPEDESPIQSNFLFYSI
                     MVITCLGLLVYGIRSFTKRDLT"
     gene            5104..5310
                     /gene="mrsA"
     CDS             5104..5310
                     /codon_start=1
                     /db_xref="GOA:P43683"
                     /db_xref="PDB:1QOW"
                     /db_xref="UniProtKB/Swiss-Prot:P43683"
                     /function="mersacidin pre-peptide"
                     /gene="mrsA"
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /product="MrsA protein"
                     /protein_id="CAB60258.1"
                     /transl_table=11
                     /translation="MSQEAIIRSWKDPFSRENSTQNPAGNPFSELKEAQMDKLVGAGDM
                     EAACTFTLPGGGGVCTLTSECIC"
     CDS_motif       5104..5229
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence:
                     MSQEAIIRSWKDPFSRENSTQNPAGNPFSELKEAQMDKLVGA"
                     /locus_tag="mrsA_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       5230..5310
                     /prepeptide="core"
                     /locus_tag="mrsA_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="-2.00"
                     /molecular_weight="2400.8"
                     /monoisotopic_mass="2399.0"
                     /core_sequence="GDMEAACTFTLPGGGGVCTLTSECIC"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MSQEAIIRSWKDPFSRENSTQNPAGNPFSELKEAQMDKLVG
                     A"
                     /leader_location="[5103:5229](+)"
                     /alternative_weights="2418.8"
     gene            5406..6047
                     /gene="mrsR1"
     CDS             5406..6047
                     /codon_start=1
                     /db_xref="GOA:Q9RC24"
                     /db_xref="HSSP:1OPC"
                     /db_xref="InterPro:IPR001867"
                     /db_xref="InterPro:IPR011991"
                     /db_xref="InterPro:IPR016032"
                     /db_xref="UniProtKB/TrEMBL:Q9RC24"
                     /function="putative response-regulator"
                     /gene="mrsR1"
                     /product="MrsR1 protein"
                     /protein_id="CAB60259.1"
                     /transl_table=11
                     /translation="MGKTLVCSENSYFQAYMNHLLTPDSNEIINVNTLDELKQIISKEN
                     FSSVIIDTCHPNDLVLQLIKSISCPVIILNSLETNVSDYNPGPILNQINNVSTLKHNVF
                     QLSFTTFFDVGKHCIWKKNEYIPLAIQEFKILYLLYLNSNKIVCSEELIEYADLTGRSS
                     LYVHISSLREKVEDNPGDPKILQTKFGKGYLLSDSTYICLEKKADSKNVV"
     gene            complement(6096..6680)
                     /gene="mrsD"
     CDS             complement(6096..6680)
                     /codon_start=1
                     /db_xref="GOA:Q9RC23"
                     /db_xref="InterPro:IPR003382"
                     /db_xref="PDB:1P3Y"
                     /db_xref="UniProtKB/Swiss-Prot:Q9RC23"
                     /function="putative mersacidin modifying enzyme"
                     /gene="mrsD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Flavoprotein"
                     /gene_kind="biosynthetic-additional"
                     /product="MrsD protein"
                     /protein_id="CAB60260.1"
                     /sec_met_domain="Flavoprotein (E-value: 3.3e-30, bitscore:
                     94.1, seeds: 143, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSISILKDKKLLIGICGSISSVGISSYLLYFKSFFKEIRVVMTKT
                     AEDLIPAHTVSYFCDHVYSEHGENGKRHSHVEIGRWADIYCIIPATANILGQTANGVAM
                     NLVATTVLAHPHNTIFFPNMNDLMWNKTVVSRNIEQLRKDGHIVIEPVEIMAFEIATGT
                     RKPNRGLITPDKALLAIEKGFKERTKHPSLT"
     gene            6892..10080
                     /gene="mrsM"
     CDS             6892..10080
                     /codon_start=1
                     /db_xref="GOA:Q9RC22"
                     /db_xref="InterPro:IPR007822"
                     /db_xref="InterPro:IPR008928"
                     /db_xref="InterPro:IPR017146"
                     /db_xref="InterPro:IPR020434"
                     /db_xref="UniProtKB/TrEMBL:Q9RC22"
                     /function="putative mersacidin modifying enzyme"
                     /gene="mrsM"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: LANC_like"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: DUF4135"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1070:lanthionine synthetase C family protein (Score:
                     1026.5; E-value: 0.0)"
                     /gene_kind="biosynthetic"
                     /product="MrsM protein"
                     /protein_id="CAB60261.1"
                     /sec_met_domain="DUF4135 (E-value: 9.3e-115, bitscore:
                     373.1, seeds: 79, tool: rule-based-clusters)"
                     /sec_met_domain="LANC_like (E-value: 9.9e-90, bitscore:
                     290.1, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHTKFKRNSVWNRSSSISERKVRRSLNTNWDELTNRRFERWKSLV
                     ESDEGIRIEDVLATQNIDEETLKHTINAKEVEFINEGDHQGWLEIIQLVDEQSYKNVNI
                     EVRKDILFFSFIKPFLKIARGKLEEVLYSHSTKSLIKEELSPSVIDDLLNNLGETLSAI
                     SSRILILELNVARVSGKLRGETSEERASYFNQALLNDPAYVRSIREEYIVLTRLLATKT
                     MYWIQNTSDLLVRFHQDKGILESEFSNGQKLGKIISIDTGSGVSDTHNKGKTVAILNFE
                     TGIKIVYKPRSLEIDVKFNKFVNYLNGKNLSFDLKTVHTLNKKSYGWTQFISYKECQEE
                     LQIGKFYWRIGSYLAILYAMNAVDFHMQNLIADGEYPILVDLESLFHNNSTYTDTSAFS
                     RAQEHIERSVLRIGLLPRKINSKAGFEGIDLSALGAQEGQVSPHKTSTIVDRDKDTVRI
                     EEKNFPIPVSQHRPMLHGQIINTVAYEGNIIKGFEETYFLFMKYKQDMLEQIDSFKGVT
                     VRQILRGTSRYANLLKISLHPDFMRDGLDREMILDKLWLDTKLNPRLNQVVNSEKEGLF
                     LGDIPYFTSKPESTNMWDSSGRKINNFFKTSALNETKEKINEMNESDCHEQVSFIKTAI
                     LVIKDSYRKHKVFDINPRLHVFNPKDFFQEAIKIGDFLASRAIEGEQLDGQEDVSWIGS
                     FVDNQREDQFKISAANSSLYEGVGGISLFLAYLGRLSNNEKYTKLSKKALVAVHKNMSA
                     SSDLGAFGGIASYLYLLDHLSKLWNDEQLLKNELYSALNKLDSLIERDENNDILTGVAG
                     TAVILINLFKRYKEEQILNLITKCGNRLIQNINVMEKGVGWKVPANPTPASGFAHGASG
                     IIWALYEIYAITKQTVFKEVAEKALEFERTLFIPEKNNWADIKLENGQFRNDNFVAWCN
                     GAAGIGLSRILILPHNQNELIKDEAHVAINTTLKYGFEHDQSLCHGDLGNLDILMYAAE
                     NFNKKLSVNVTELSHKILNDIKLRGWLTGFEKNNESPSLMMGYAGIGLGLLKIFAPVEV
                     PSVLRLQSPLELKL"
     gene            10132..12324
                     /gene="mrsT"
     CDS             10132..12324
                     /codon_start=1
                     /db_xref="GOA:Q9RC21"
                     /db_xref="HSSP:1MT0"
                     /db_xref="InterPro:IPR001140"
                     /db_xref="InterPro:IPR003439"
                     /db_xref="InterPro:IPR003593"
                     /db_xref="InterPro:IPR005074"
                     /db_xref="InterPro:IPR011527"
                     /db_xref="InterPro:IPR017871"
                     /db_xref="InterPro:IPR017940"
                     /db_xref="UniProtKB/TrEMBL:Q9RC21"
                     /function="putative ABC-transporter"
                     /gene="mrsT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288:ABC
                     transporter related protein (Score: 278.6; E-value:
                     1.8e-84)"
                     /gene_kind="transport"
                     /product="MrsT protein"
                     /protein_id="CAB60262.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 1.4e-44, bitscore:
                     140.6, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRRRVPLVRQMGQYECGPACLTMILSYYGSTISLNKISEQCDAQR
                     NGVSVSILKSVSEYYGLNCKVYQVSFKDLKKYINSYLPCIIFWDERHFVVLEQIKKGLF
                     HIIDPNRGKLKLSEEEFKRHYSKVILTFKKSDKFKEMMPSPAAKYYLRYIVKSRTIVSL
                     IILFSLITQVVFLAVPFLIKYLVDHSLISKSTNSFLFLGIVVIIAVFILGLVMFIRNYF
                     TIKLQAIISKSISNDFVEHLLKLPLNFYENRTTGDIAMRVSNISMIREIIAKNGATIVL
                     DIITLISFFIAMLTQSFKLAFFAIGLAIIQFLLMMILIPRIKNLIHNDLSIQTTTQSFL
                     VEALRAITFIKSNGLDHSILTKWSNYYDKQIEAFSQRYHLDAIMDSISVSIRYCAPLLL
                     LWFGSKEVITGNLTLGGLLGFSSLGTSFLLPIASLITGMQQFQLVGDTFERMQDVMETE
                     PEQINQTSIIETELSKQDIKLENVTFTHHSLHILKEVSLNIKSGTKLALVGRTGSGKTT
                     LSRIILGLYKPTKGKVFYGEQDLKNLNLYELRKQMGVVLQESFLFNDTIANNIAGFKSL
                     SQDKIEQAAKRVQLHEDIIRMPMGYNTIIGENGSMLSGGQRQRIAIARAIVDNPSVVIL
                     DEITSNLDTLTEHEIDEYFAKSNITRIVITHRLLSSQDSDLIVVLDQGKIVEKGKHQEL
                     LEKKGYYYNLWIKQVGDRQKATLQKPFFLNEQTGKT"
ORIGIN
        1 ctaattgaca ttgttatact tagatggcgg actcggaaag gtaattttaa atcttgtgcc
       61 aattcccggc tgactatata tatcaatatt tcccccatgt gccttgataa tttgttgagc
      121 aatagccatt cctaatccag tccctgagtt atttgacgta gcgcttgtcc ctctgaaata
      181 ccgatcaaat aaacattcga ccgtctcttc gtccattcct atcccatcgt cttctattat
      241 aatttgtatt tcattgtcat gtaacacgtc ctgatataat atgaccttta ttttagtgcc
      301 cggaggatta tgtttaatac aattagcaat caagttttca actgcacgct ttaggtacct
      361 ttcatccata ttaaaaagta tcttattgtg actggattct aaagcgaatc tcttattttc
      421 tgactccggt agcagcttca ttttttctaa agtgtcatga attactttca ctacgttttt
      481 ttcttcaaga tggattggaa gcgactcatt tttcaattga aaagttaagt tgaaatcttc
      541 aatcagcttt tccatatatt cgacacgttc ttccattatt aaggaaaaat cacgcacttg
      601 ctgattttcc cactcatatt catttgaaga taaaagaacc gtatatccct taataaccga
      661 aagaggggtt tttaaatcat gtgagacacc ggccatccac tcttctcgtg tcttttccaa
      721 taattctcgc tcagccttat tcctttgaag cgttgaggta agttcagaca gagcctgcat
      781 taattcttga taggtcttat atccagattt attatgtcta tatttgcttg aatgaaactt
      841 ccaatcataa ggttcttcat atttttcttt agacaaattc tcaatccaag atacaataaa
      901 cagcagggga gcacctagtt tttttccgta aaatattgca atgacaatcc ctgccataat
      961 aatagaaaca atccacaagt tattcaccca atagaaaaaa gggttatttt tttccatcgg
     1021 ctttcccaat acccatgtta aatcctgatt gtttttcgtt tcataccacg tagataattg
     1081 ataacctttt ttggcaggat acatgtaatc tgatattaac tctccaagaa tataatgctt
     1141 aggtatattt tgaggtttat taaaagaaaa aacctcatct ccttgttcat ctaaaatttg
     1201 aatccacatc cggttctcta ataaatcttc ttttgctttc gtatttattg aaaattgttt
     1261 atctgcaaca actgtttggt caacaatttt ttttaaggat aagtgcggcg tttccatttt
     1321 attctgacct aagacgaggc tgaaaaatat gatactgccc acaatgagca caccccaaat
     1381 aaacatgagt aaaaaaagtc tcgatacgaa gtaaaatgct atcctatttc tcagcttcaa
     1441 aataaatcct catctatagg ctctgcttca aatatatagc cctgtcctcg aaccgtttta
     1501 atccatctag gtctgctcgg gtcttcctct aatttttcac gtaatcgtct tacatgtacc
     1561 attacagtac tatctccgcc ataaaactcc ccccacacat ctttataaat ttggtgttta
     1621 cttaaaattt gattgggatg ttcacagaaa taaatcagaa gtttcatttc ctgagcaggg
     1681 caatctattc ttgtcccgtt aacaacaagt cttccggtat tgggatctat tttaaaatat
     1741 tcataatcgt atacctgttt tagagggtaa gaagaagaga gttgagtcgt tctctttaat
     1801 tgggctttta cacgagctac aatttctagt ggattaaatg gtttagtaat atagtcatct
     1861 cccccaaaac taaacccttg caatttatct aaatctgttg tttttgccgt taagaagagt
     1921 atcggcacat ttgtatgcga gcgtattttg ctgcataagg tgaagccatc agtgtccgga
     1981 agcatgacat ctaataaaat aatatttgga agttcctgat cgattttcag aagggtttcg
     2041 ctccctgtca tagctgtgga aatattgctg aaaccttcct tttcaaagca agttattaat
     2101 aagtttaaga tatgttcgtc atcatccacc atgaggattt tgttctcttc taacgtcata
     2161 agttaccctc ctttttattt cttattttat atgagatcaa tgacataaag gtcagaaaag
     2221 gaaagaattt aactttcgtt aagggttcag aatattcttt cgaattttta accttgatat
     2281 tcaggtatca atataactac atacagaaaa taaaccaggg atgtcgttaa gtgaatgttt
     2341 aggaagagtt tcgtttgtgt ttaaagaaca tcgcttatga taaaagtgag gtgagagaca
     2401 aatgaatgat caaatagtgg taacacacga tctgactaaa aagtataaaa agcatacttc
     2461 tgttgatgga ttaaacttaa ggattaggcg tggcgaaatt tatggttttc ttggaccgaa
     2521 tggtgctggt aaaacaacaa ccatccgaat gttattaggt ttaattaaac caacaaaagg
     2581 aaacatcgaa atctttggcc aaaacctaaa caagaatcgt ttgcagatat tgcaaagaat
     2641 cgggtcactg gtagaatctc caacttatta tggtaattta acaggttacg aaaacttaga
     2701 agctgttagg aggttacgag gacttccgga acaacaggtc aatgaagtat tggaaactgt
     2761 aaggttatct aaagtagcaa atcgactgac taaggagtat tcccttggaa tgaaacaacg
     2821 tttgggaatt gcagttgccc tgttaagcag tcctgattta ttaattctgg atgaaccgac
     2881 aaatggttta gatccatccg gtatccaaga aataagagag ctaattaaag aattgcctaa
     2941 atcgggaatg agtgttattg tatccagcca cttattgagt gaaatagatc aaatggctac
     3001 tcaagtggga attatcaaca atggaaaaat gatttttcag gactcgattg caagtttaca
     3061 tcaaaaaaga aaaccactat taaaagtcgg tgttagtgac gtaatcgaag caaaaacaat
     3121 attaaacagg aaaggattaa aggttgattt acaaaaaaat tatttgtggc tgtctcaaac
     3181 agaaccggaa ttcgtttcag aaatcaattc catacttctt cattcagggc tgtctgtatt
     3241 tcgacttgaa gaaaagacac gatcacttga ggatattttt ttagaattaa ccggtacaga
     3301 gggaagtcta tgaaaaagtt attatgggca gatcaattaa aactgaaacg ttcgtcgtta
     3361 ttgattgtag tcttattggt tcccttactc attatagcat atgagttggt aaatcttact
     3421 tatcgatctg aatacgtgga aaaacaagct gaaatgttcc atgctggatc aatgtggatg
     3481 tatttactgt atgataacag tttgttattt ggtctgggtt ttccattagc cgccacactt
     3541 tctgcgtcaa taatagcaaa tatagagcat caagcaaacg gatggaagca aaccctttct
     3601 tttcctgtat ctagaatgcg aatttatgta agtaaattta tttgcttagt agtcagttta
     3661 tttatttcgt caacaatctt cttgctcggc atggtactgc tgggcaaact ggtaggattc
     3721 gaaggtagtg taccttgggg acttttgttt ggagatagtt acagcatgtt agttacagta
     3781 ctgcctataa tggcattcca gatatggtta tctatggtgt ttagcaacca agcattctca
     3841 attcttgtag gatcagtatc atctataatg ggtttgtttt tggcagctgc tcaatcaacg
     3901 agatggtttc cgctggctta tcctagtcaa tcttcgacag tcattctgca gtatgaaggt
     3961 ataggatata acccagatct ttcatcttac ctttgcatta gccttttctt agggataatt
     4021 atattatttc taggctctat tcattttgct aaacgggatg ctttgtaaaa aggaggcgaa
     4081 atacttgaaa aacattttat ttgtagaacg tttaaagctt aagcgatcaa agttatggat
     4141 catctactta ttagggcctt tgttaggtgt atccctggca tacactaact ttattaaaaa
     4201 ctataacctt tttatgaatc ccggagacaa tccctgggta gaggcttgga cacaagttgc
     4261 cttatttatg ggcccttttg tattgcctat cgtagtggga attttcgccg cccttgtttg
     4321 caggggggaa catgtaggag gcggttggaa acagcttcta gccttaccag tcaaacactc
     4381 agatatcttt ctgggaaagt ttctaacagt ggtccgcatg atattcatta gtatgtccat
     4441 tttaatcctc ttatttattg gattcggtta tatgttaggc ataagcggga gtcttccttt
     4501 acttacaatt ctaggttatg gaattagagg gatcttagct tgtttaccat taattttatt
     4561 gcagcttatt gtttcaatca ggtctaaaac atttggcata ccactcgctg ttagcattgt
     4621 ttttacatta ccggccatta ttattgccag tacaccatta ggtcaagtat atccatggac
     4681 acagcctatg ttagcaatgt cacccgaaga tgaatcaccg attcaatcaa atttcctgtt
     4741 ttactcaatt atggtaataa cgtgtctcgg tctcctggtt tacggaataa gaagttttac
     4801 taaacgagac cttacgtagg gtatatgcgg tataaactta tgagaattcg agacaaggta
     4861 aactaatttg actagcgcta tgtaaaacag aaatttatat gaaggtaaga ctaaatcttt
     4921 ttggttgtat tctttgaaac taaacgacta atggaaaagg tgatgtttca aagcataaga
     4981 cataagaaat ataaagatat cttaagactc tttatttaaa cattttttac atttaataat
     5041 atctcttcca tttttttgat tatgagttaa tataatacta tacttaataa gggggtgaat
     5101 acaatgagtc aagaagctat cattcgttca tggaaagatc ctttttcccg tgaaaattct
     5161 acacaaaatc cagctggtaa cccattcagt gagctgaaag aagcacaaat ggataagtta
     5221 gtaggtgcgg gagacatgga agcagcatgt acttttacat tgcctggtgg cggcggtgtt
     5281 tgtactctaa cttctgaatg tatttgttaa tttgatttat ataggctgtt tcccttcaga
     5341 aggaacagcc tatattttat tatataaact attagaaaat tcttaaaaaa caggagggta
     5401 aatcattggg gaaaactctc gtttgttcag aaaatagcta tttccaagca tatatgaatc
     5461 atttattaac gccagatagt aatgaaataa taaatgttaa cacattggat gaactaaaac
     5521 aaataatatc caaagaaaat ttttcctctg taatcatcga tacttgccac cctaatgact
     5581 tagtgttgca actgataaaa tcgatatctt gcccagtcat aatacttaac tccttagaaa
     5641 ctaacgtttc agattataac cctggtccaa tattaaatca aataaataat gtttcaaccc
     5701 taaaacataa tgtttttcaa ttgtctttca caactttttt cgacgttgga aagcattgta
     5761 tttggaaaaa gaatgaatat atccctctag caatacaaga atttaaaatt ttatatttgc
     5821 tttatttaaa ttcaaataaa atagtctgtt ctgaagaact tattgaatat gctgacctta
     5881 ctggtaggtc aagtctttat gtacacatta gttctcttag agaaaaagta gaggataacc
     5941 ctggagatcc aaagattctt caaacaaagt ttggaaaagg gtacctctta tctgacagta
     6001 catatatttg tcttgaaaaa aaagcagact caaaaaatgt cgtgtaaaaa ggatgaccca
     6061 aaaatcattt taaaatgact tttgagtcat cccatttatg ttagtgaggg gtgttttgtt
     6121 ctttctttaa aacctttttc tattgctaat aaagctttgt cgggtgtaat tagaccacga
     6181 ttaggttttc ttgtacccgt cgcaatctca aacgccataa tttcaactgg ttcaataaca
     6241 atatgcccat cttttcttaa ttgttcaata ttcctggaaa caactgtttt attccacatt
     6301 aaatcgttca tattaggaaa gaaaatagtg ttatgtggat gagctaatac agtagttgca
     6361 actaaattca ttgctacacc attagcagtt tgtcctaaga tgttcgctgt agctggaata
     6421 atgcaatata tgtccgccca gcggccaatt tctacgtggc tatgtctttt tccgttttcc
     6481 ccatgttctg agtatacatg gtcacaaaaa taagaaacag tatgagctgg aataaggtct
     6541 tccgctgttt ttgtcataac aacccttatt tccttaaaga agcttttaaa atataagaga
     6601 tacgaggaga tgccgacaga tgagattgat ccgcaaatcc cgatcaataa ctttttatct
     6661 tttaatattg aaatactcat ttttttctcc ctctgtaaat tttaaacaat catactacac
     6721 aattattctt tttgtggtgt ttattccaaa tattaaatat tcttaataaa cacatataaa
     6781 ataaacctaa acttctttta aaattctttt aacttttaaa tggctctgct atatttatgg
     6841 ataattaagg aatattaatt gttcaaattt attaaaaatg aggtgttcaa tatgcataca
     6901 aaattcaaac ggaattcggt atggaatagg tcttcttcga taagtgaaag aaaagtaagg
     6961 cgttcactta atactaattg ggatgaacta acaaacagac gcttcgaaag atggaaaagc
     7021 cttgttgaaa gtgatgaggg gattcgaatt gaggatgtat tagccaccca aaatattgac
     7081 gaagaaaccc taaagcatac tatcaatgca aaagaagtgg aatttattaa tgaaggtgat
     7141 catcaaggat ggcttgaaat tattcagcta gttgatgaac aatcctataa aaatgtaaat
     7201 atagaagtta gaaaagatat tctcttcttt agttttataa aaccattttt gaaaatagca
     7261 agaggcaaat tagaggaagt attatattct cactctacta aatctctgat aaaggaagaa
     7321 cttagtccgt cggtaattga tgatttactt aataatcttg gtgaaacttt atcagctata
     7381 agtagtcgga tattaatatt ggagttaaat gtagcaaggg tatcaggaaa gctgcggggt
     7441 gaaacttctg aagaacgagc ctcttatttc aatcaagccc ttttaaatga cccggcatat
     7501 gttcgatcaa ttcgagaaga atacatagtt ttaacaaggc tgttagcaac aaaaacaatg
     7561 tattggattc aaaatacttc tgatctcctt gtaagatttc atcaggataa agggatatta
     7621 gaatctgagt ttagtaatgg tcaaaaatta gggaaaatta tttccatcga tacaggaagt
     7681 ggcgtctcag atactcataa caaaggaaaa acagtcgcca ttttaaactt tgaaactgga
     7741 attaaaattg tgtataagcc tcgttcatta gaaatagacg taaaatttaa taaatttgta
     7801 aattatctaa atggtaaaaa tttaagtttt gacttaaaaa ctgtacacac acttaacaaa
     7861 aaatcttatg gatggacgca gtttatctct tataaagaat gtcaggaaga actgcaaatt
     7921 ggaaagtttt attggcgtat tggaagttat ttggctatct tatatgcaat gaatgcggta
     7981 gattttcata tgcaaaattt aattgctgat ggagaatatc ctattttagt tgatttagaa
     8041 tctttatttc ataataattc tacgtataca gataccagtg cttttagtcg cgcacaagaa
     8101 catattgaac ggtcagtttt acgaattggg ttattaccga gaaaaataaa tagtaaagct
     8161 ggatttgaag gaattgacct tagtgcatta ggtgcacaag aaggacaagt atctccgcat
     8221 aaaaccagta caatagttga tcgagataaa gatacagtca ggatagaaga aaaaaacttt
     8281 ccaattccag ttagtcagca tagacctatg ctgcatggac aaatcattaa tactgttgct
     8341 tacgaaggaa atattataaa aggatttgaa gaaacctact tccttttcat gaaatataaa
     8401 caagacatgc tcgaacaaat agattctttt aaaggggtta ctgtaagaca aatattgcgt
     8461 ggtacatctc gctatgcaaa ccttctgaaa ataagtttac atccagattt catgagagac
     8521 ggcttagatc gtgaaatgat tttagataaa ttgtggctag atacaaaatt gaatccacga
     8581 ttgaaccaag ttgtaaatag tgaaaaggaa ggcttattcc ttggggatat cccatatttc
     8641 acaagcaagc ctgaatctac aaatatgtgg gattccagcg gtcgaaaaat caacaatttt
     8701 tttaaaacaa gtgccttaaa tgagacaaag gaaaaaataa atgaaatgaa tgaaagtgac
     8761 tgtcatgaac aagtaagctt tataaaaaca gctattttag taattaagga ttcttatcgt
     8821 aaacataaag tatttgatat aaatcctcga ttacacgttt ttaacccaaa agattttttt
     8881 caagaagcta ttaaaatcgg agactttctt gctagtaggg cgattgaagg tgaacagtta
     8941 gatggacaag aggatgtatc ttggatcgga tcgtttgttg acaatcaacg agaggatcaa
     9001 ttcaagattt cagctgcaaa tagttcatta tacgagggtg taggtggtat ttctctcttc
     9061 cttgcttatt taggtcgcct ctctaacaat gaaaaataca ccaaactatc taaaaaagct
     9121 ttagtggcag tacacaaaaa tatgtctgca tctagtgatt taggtgcctt tgggggaatt
     9181 gcttcttacc tttatttgtt ggatcattta tcaaaattat ggaatgatga acaactattg
     9241 aaaaacgaac tttattctgc cctcaacaaa ttagattctt tgatcgaaag ggatgaaaac
     9301 aacgatattt taacaggggt ggctgggaca gcagttattt taataaattt gtttaaacgg
     9361 tataaagaag aacaaatctt aaacttgatt acaaaatgcg gaaatcgtct tattcaaaat
     9421 ataaacgtta tggaaaaggg agttggatgg aaagtcccgg caaacccaac gccagcctcc
     9481 ggatttgctc atggtgcctc aggaattata tgggctcttt atgagattta cgcaattact
     9541 aagcaaactg tatttaaaga ggtagctgaa aaagcgttag aatttgaaag aactttgttt
     9601 attccggaaa aaaacaattg ggcagatatt aaacttgaaa acggacagtt tcgaaatgat
     9661 aattttgttg cttggtgtaa tggcgcagca ggcataggat taagtaggat attgatcctg
     9721 ccacacaatc aaaatgaatt gataaaagat gaagcacatg tcgcaattaa tacaacccta
     9781 aaatatggtt ttgaacatga tcaatcttta tgccatggtg atttaggtaa tctggacatc
     9841 cttatgtacg cagcggaaaa ctttaataaa aagttaagcg taaatgtaac agaactaagc
     9901 cataaaattt taaatgatat aaagctcaga ggatggttaa ctggatttga aaaaaataac
     9961 gaatccccat ccttaatgat ggggtatgca ggtataggac ttggattgct taagattttt
    10021 gcaccagtcg aagtgccatc agttttgaga ctccaatcac ctttagaact aaaattgtaa
    10081 aattaaaatg agccttactt tgttaaggct ctatttctta gaggtgatcc aatgagaaga
    10141 agagttcctc tagtaaggca aatgggacaa tatgagtgtg ggcccgcctg tctcaccatg
    10201 attttaagtt attatgggag tactatatct ctaaataaaa taagtgaaca atgtgatgcc
    10261 caaagaaatg gcgtatctgt atccatttta aaatcagtat cagaatatta tgggcttaac
    10321 tgcaaagtat accaagtttc gtttaaagat ttgaaaaaat atatcaattc atatcttccc
    10381 tgcatcatat tttgggatga acggcacttt gtggttttag aacagattaa aaaaggtctt
    10441 tttcatataa ttgaccccaa cagaggaaaa ttgaaattat ccgaagaaga atttaaaaga
    10501 cattatagca aggtaatctt gacttttaag aagtcagata aatttaaaga gatgatgcca
    10561 tcacccgcag caaagtatta tttacgttat atagttaaaa gccgtactat tgtttcactt
    10621 atcattctct tttcactgat cacacaagta gtcttcttag ccgttccttt tttaattaaa
    10681 tatttagtgg atcattcttt aataagtaaa tcaacaaatt cttttctatt tttaggaatt
    10741 gtagtcatca tagcagtttt tatattggga cttgttatgt ttatccgcaa ttatttcaca
    10801 attaaacttc aagctattat aagtaaaagc atctcaaacg attttgtgga acatttatta
    10861 aaattacctc tcaattttta tgaaaatcga acaaccggtg atattgcaat gagagtaagt
    10921 aacatttcca tgataaggga gattatagct aagaatggag caacaatagt actagatatt
    10981 attactttga tttcattttt tattgctatg ttaactcaat catttaaact tgcatttttt
    11041 gctattggat tggcaattat tcaattctta ctaatgatga ttttgattcc aagaataaaa
    11101 aatttaatcc ataatgactt atcaattcaa acaaccacac aaagtttttt agtggaagct
    11161 ctaagagcta tcacttttat taaatcaaat ggtttagatc attctatcct aacaaagtgg
    11221 tcaaattact atgataaaca aatcgaagca ttttcacagc gataccactt agacgctata
    11281 atggatagca taagtgtaag catccgatat tgtgcacccc ttctcttatt atggtttgga
    11341 tcaaaagaag ttatcaccgg aaatttaaca ctcggaggct tattagggtt tagcagctta
    11401 ggaacatctt tcttgttgcc gattgcttct ttaataacag gcatgcaaca atttcaattg
    11461 gtcggtgaca cttttgaacg aatgcaagat gttatggaaa ctgagcccga acaaatcaac
    11521 caaacttcaa taattgaaac tgaactatca aaacaagata taaaacttga aaatgtcaca
    11581 tttacacatc atagcttaca tattcttaaa gaagtttcat taaacattaa gtcaggtact
    11641 aaattagctc tcgtaggccg tacaggtagt ggaaagacaa ctttatcaag aattatactg
    11701 gggttataca aacccactaa gggaaaagta ttttatggtg aacaggactt gaagaattta
    11761 aacctttacg agctccgaaa acaaatgggg gtcgtattac aagaaagctt tcttttcaat
    11821 gatactattg caaacaatat agctggattt aaaagcttat ctcaggataa aattgaacaa
    11881 gcagcaaaga gagtgcagtt acatgaagat ataataagaa tgccaatggg atataacaca
    11941 attatcgggg aaaatggcag tatgttatca ggagggcagc gtcagcgcat agcaatagca
    12001 agagctatcg tcgataaccc ttctgtagtc attttagatg aaataacaag taacttggat
    12061 acattaacag agcatgaaat tgacgaatac tttgcaaaat caaatattac ccgtattgta
    12121 ataactcatc gtcttttaag tagccaagac tctgatttaa tagtggtatt agatcaagga
    12181 aaaatagtcg aaaaaggaaa acatcaagag ttattagaga aaaagggata ctattataac
    12241 ttatggataa aacaagtagg ggatagacag aaagctacgc tgcaaaagcc ctttttcctc
    12301 aatgagcaaa ctggcaaaac ttga
//
