LOCUS       BGC0000528              8183 bp    DNA     linear   BCT 27-FEB-2015
DEFINITION  Clavibacter michiganensis subsp. michiganensis NCPPB 382 complete
            genome.
ACCESSION   BGC0000528
VERSION     BGC0000528
KEYWORDS    .
SOURCE      Clavibacter michiganensis subsp. michiganensis NCPPB 382
  ORGANISM  Clavibacter michiganensis subsp. michiganensis NCPPB 382
            Bacteria; Actinobacteria; Micrococcales; Microbacteriaceae;
            Clavibacter.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-07 19:46:36
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 8183
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..747)
                     /codon_start=1
                     /db_xref="EnsemblGenomes-Gn:CMM_1962"
                     /db_xref="EnsemblGenomes-Tr:CAN02018"
                     /db_xref="UniProtKB/TrEMBL:A5CSF4"
                     /function="Uncharacterized protein conserved in bacteria"
                     /gene="clvE"
                     /locus_tag="CMM_1962"
                     /note="conserved membrane protein (ZP_00099616.1| COG4200:
                     Uncharacterized protein conserved in bacteria
                     [Desulfitobacterium hafniense]; CAB60257.1| MrsE protein
                     [Bacillus sp. HIL-Y85/54728]; NP_721536.1| putative
                     transporter, trans-membrane domain bacteriocin immunity
                     protein [Streptococcus mutans UA159]). PUTATIVE MEMBRANE
                     PROTEIN INVOLVED IN LANTIBIOTIC IMMUNITY; Function unclear"
                     /product="conserved membrane protein"
                     /protein_id="CAN02018.1"
                     /transl_table=11
                     /translation="MFRAEAVKLKRSSIWIIAVLLPLLAVVTGTINLANNTQTLDSGWA
                     SFTSQVVLFYGLLFYSMGISLLAAAIWRVEHRGTNWNALLTATARPIRLVLAKIVAILV
                     PVALMQAVLVGGTAVSGLFVLRLDGPFPVAFLLVGLIATVAALPLIAVQSLLSLLLRSF
                     AAPVALCLVGCVAGVAAVTSAALRPLAEVLPQAINTRALNLGSTAIAGSGGLTTADTLP
                     LLATSVGLGALVVLLTLAAIRVVKLR"
     protocluster    1..8183
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[5021:8183]"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5022..8183
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
     cand_cluster    1..8183
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(LANC_like and DUF4135)"
                     /kind="single"
                     /product="lanthipeptide-class-ii"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..8183
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000528"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..8183
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lanthipeptide-class-ii"
                     /region_number="1"
                     /rules="cds(LANC_like and DUF4135)"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(1..747)
                     /gene="clvE"
                     /locus_tag="CMM_1962"
     gene            complement(753..1505)
                     /gene="clvG"
                     /locus_tag="CMM_1963"
     CDS             complement(753..1505)
                     /codon_start=1
                     /db_xref="EnsemblGenomes-Gn:CMM_1963"
                     /db_xref="EnsemblGenomes-Tr:CAN02019"
                     /db_xref="UniProtKB/TrEMBL:A5CSF5"
                     /function="Immunity to Lantibiotics"
                     /gene="clvG"
                     /locus_tag="CMM_1963"
                     /note="conserved membrane protein (ZP_00099615.1| COG1277:
                     ABC-type transport system involved in multi-copper enzyme
                     maturation, permease component [Desulfitobacterium
                     hafniense]; CAB60256.1| MrsG protein [Bacillus sp.
                     HIL-Y85/54728]). PUTATIVELY INVOLVED IN IMMUNITY TO
                     LANTIBIOTICS; Specificity unclear"
                     /product="conserved membrane protein"
                     /protein_id="CAN02019.1"
                     /transl_table=11
                     /translation="MRRAVAIEFRKMHRLRSLPLLIGMVVAVAALSSASQFAGSTRAGF
                     DDPGAHPWAALLLGYTLMAAMTSPILTAVIASRQTDIEHQAGGWILAAGAGRTAGELCR
                     AKLVALAVLLVPAVLIQSLLVIAAGYAAGIRVPVEVGPWALYTLLLYLVDVAFCALHVW
                     LAARVENQLISVGVGMLGAFLAVFSLLLPSVASRAIPWGYYAVISQAGQSDAGVGYVAS
                     PLGWIAGFLVVVAAAFALATIRLDRVER"
     gene            complement(1502..2401)
                     /gene="clvF"
                     /locus_tag="CMM_1964"
     CDS             complement(1502..2401)
                     /codon_start=1
                     /db_xref="EnsemblGenomes-Gn:CMM_1964"
                     /db_xref="EnsemblGenomes-Tr:CAN02020"
                     /db_xref="GOA:A5CSF6"
                     /db_xref="InterPro:IPR003439"
                     /db_xref="InterPro:IPR003593"
                     /db_xref="InterPro:IPR017871"
                     /db_xref="InterPro:IPR027417"
                     /db_xref="UniProtKB/TrEMBL:A5CSF6"
                     /function="ABC-type multidrug transport system ATPase
                     component"
                     /gene="clvF"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 174.2; E-value:
                     5.7e-53)"
                     /gene_kind="transport"
                     /locus_tag="CMM_1964"
                     /note="putative ABC transporter, ATP-binding protein
                     (CAB60255.1| MrsF protein [Bacillus sp. HIL-Y85/54728];
                     ZP_00099614.1| COG1131: ABC-type multidrug transport
                     system, ATPase component [Desulfitobacterium hafniense]).
                     PUTATIVELY INVOLVED IN IMMUNITY TO LANTIBIOTIC.
                     pfam00005,ABC_tran, ABC transporter. InterPro: AAA ATPase
                     superfamily.; Specificity unclear"
                     /product="putative ABC transporter, ATP-binding protein"
                     /protein_id="CAN02020.1"
                     /transl_table=11
                     /translation="MSAVIATRGLTKRFRDHVAVDALDIEVPQGSVYGFLGPNGSGKST
                     TMKMLLGLTQPTSGEIHLFGQRLTPATRGGLLPSIGSMIEAPAGYGHLTGWENMRIVRD
                     MLGLAEAQVERAFATVRLTQHRDKLVRRYSLGMKQRLGIAMALARDPSLLVLDEPTNGL
                     DPAGIEEVRSLLMDLAGQGITVMVSSHLLDEIEKMAGVLGILADGRMIFQGTRAELFEH
                     SIPDLVIETSAPDRAMAEVAGAARSPEGIRLSARSKDETADIVRRLVAADVPVHEVRRL
                     PQSLEDVFMDITGRGGQL"
     gene            complement(2555..3229)
                     /gene="clvR"
                     /locus_tag="CMM_1965"
     CDS             complement(2555..3229)
                     /codon_start=1
                     /db_xref="EnsemblGenomes-Gn:CMM_1965"
                     /db_xref="EnsemblGenomes-Tr:CAN02021"
                     /db_xref="GOA:A5CSF7"
                     /db_xref="InterPro:IPR000792"
                     /db_xref="InterPro:IPR001789"
                     /db_xref="InterPro:IPR011006"
                     /db_xref="InterPro:IPR011991"
                     /db_xref="InterPro:IPR016032"
                     /db_xref="UniProtKB/TrEMBL:A5CSF7"
                     /function="Response regulator containing a CheY-like
                     receiver domain and an HTH DNA-binding domain"
                     /gene="clvR"
                     /gene_functions="regulatory (smcogs) SMCOG1016:LuxR family
                     DNA-binding response regulator (Score: 167; E-value:
                     5.6e-51)"
                     /gene_kind="regulatory"
                     /locus_tag="CMM_1965"
                     /note="putative two-component system, response regulator
                     (NP_625929.1| putative two-component response regulator
                     [Streptomyces coelicolor A3(2)]; ZP_00227053.1| COG2197:
                     Response regulator containing a CheY-like receiver domain
                     and an HTH DNA-binding domain [Kineococcus radiotolerans
                     SRS30216]). PUTATIVELY INVOLVED IN BIOSYNTHESIS OF
                     LANTIOTIC pfam00072, Response_reg, Response regulator
                     receiver domain. pfam00196, GerE, Bacterial regulatory
                     proteins, luxR family. InterPro: Response regulator
                     receiver domain.; Specificity unclear"
                     /product="putative two-component system, response
                     regulator"
                     /protein_id="CAN02021.1"
                     /transl_table=11
                     /translation="MTDAPAPIRVVVVDDQSLVRDGFARIVDAQPDMAAVGVCADGEQA
                     VARVVELRPDVVLMDVRMPVLDGIEATRRLVEGGRAEGTRILGLTTHDTDSYAIRMLRA
                     GAVGFLLKDSTAVQLVDAIRSVHNGTFAASMSTTQRLLERLAVERTAPPDPDTAALDVL
                     TDRERVVFTRLVTGASNPEIARDLSIAEVTVKTHVGHILTKLAVRDRVHLVIWAHRRGL
                     AA"
     gene            complement(3226..4362)
                     /gene="clvK"
                     /locus_tag="CMM_1966"
     CDS             complement(3226..4362)
                     /codon_start=1
                     /db_xref="EnsemblGenomes-Gn:CMM_1966"
                     /db_xref="EnsemblGenomes-Tr:CAN02022"
                     /db_xref="GOA:A5CSF8"
                     /db_xref="InterPro:IPR003594"
                     /db_xref="InterPro:IPR011712"
                     /db_xref="UniProtKB/TrEMBL:A5CSF8"
                     /function="Signal transduction histidine kinase"
                     /gene="clvK"
                     /gene_functions="regulatory (smcogs) SMCOG1048:sensor
                     histidine kinase (Score: 138.8; E-value: 4.1e-42)"
                     /gene_kind="regulatory"
                     /locus_tag="CMM_1966"
                     /note="putative two-component system, sensor kinase
                     (NP_825857.1| putative two-component system sensor kinase
                     [Streptomyces avermitilis MA-4680]; ZP_00228428.1| COG4585:
                     Signal transduction histidine kinase [Kineococcus
                     radiotolerans SRS30216]). PUTATIVELY INVOLVED IN
                     BIOSYNTHESIS OF LANTIBIOTIC InterPro: Histidine kinase-DNA
                     gyrase B- phytochrome-like ATPase.; Specificity unclear"
                     /product="putative two-component system, sensor kinase"
                     /protein_id="CAN02022.1"
                     /transl_table=11
                     /translation="MRRGSLRAQGDQVVAGAAVVLCAIMGVLGQPDWIWSSLMALTLAV
                     RHARPVTAALLATAISSAHMLATSSLLFPGDVVLLVAAYSVAAHAADRLRHVGLALGAC
                     FVVVLAARTLTGGELGSTGSVGMVIALVSVSFAVAWAAGLLERRRSEAVRTAELRTTLS
                     ERDADARTRLAAYEERERISDDMHDVLAHTLTSVIVQAESGRATAPSPEASRMFRTISE
                     TSRAALHEVRGLLAPTDADADVRPIPGWDDVDALVEGFRGSGLGIDRQDAGSPRPLLPG
                     VGFAVYRVVQESLTNAMRHGAGGTARLRVEWSPDALAITVANAVVRDKRGCPVQEHRGL
                     AAIRRRCALYGGQARYACDDEFVLQARWPTAPLGGSTP"
     gene            4724..4930
                     /gene="clvA"
                     /locus_tag="CMM_1967"
     CDS             4724..4930
                     /codon_start=1
                     /db_xref="EnsemblGenomes-Gn:CMM_1967"
                     /db_xref="EnsemblGenomes-Tr:CAN02023"
                     /db_xref="UniProtKB/TrEMBL:A5CSF9"
                     /function="Lantibiotic precursor"
                     /gene="clvA"
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="CMM_1967"
                     /note="putative lantibiotic (clavicidin) precursor
                     (LANA_ACTLG Lantibiotic actagardine (Gardimycin)). MAY
                     INHIBIT PEPTIDOGLYCAN SYNTHESIS; Function unclear"
                     /product="putative lantibiotic precursor"
                     /protein_id="CAN02023.1"
                     /transl_table=11
                     /translation="MNDILETETPVMVSPRWDMLLDAGEDTSPSVQTQIDAEFRRVVSP
                     YMSSSGWLCTLTIECGTIICACR"
     CDS_motif       4724..4864
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence:
                     MNDILETETPVMVSPRWDMLLDAGEDTSPSVQTQIDAEFRRVVSPYM"
                     /locus_tag="CMM_1967_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       4865..4930
                     /prepeptide="core"
                     /locus_tag="CMM_1967_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="-3.60"
                     /molecular_weight="2109.5"
                     /monoisotopic_mass="2108.0"
                     /core_sequence="SSSGWLCTLTIECGTIICACR"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MNDILETETPVMVSPRWDMLLDAGEDTSPSVQTQIDAEFRR
                     VVSPYM"
                     /leader_location="[4723:4864](+)"
                     /alternative_weights="2127.5"
                     /alternative_weights="2145.5"
     gene            5022..8183
                     /gene="clvM"
                     /locus_tag="CMM_1968"
     CDS             5022..8183
                     /codon_start=1
                     /db_xref="EnsemblGenomes-Gn:CMM_1968"
                     /db_xref="EnsemblGenomes-Tr:CAN02024"
                     /db_xref="InterPro:IPR007822"
                     /db_xref="InterPro:IPR017146"
                     /db_xref="InterPro:IPR025410"
                     /db_xref="UniProtKB/TrEMBL:A5CSG0"
                     /function="Lantibiotic modifying enzyme"
                     /gene="clvM"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: LANC_like"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: DUF4135"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1070:lanthionine synthetase C family protein (Score:
                     701.5; E-value: 3e-212)"
                     /gene_kind="biosynthetic"
                     /locus_tag="CMM_1968"
                     /note="putative lantibiotic modifying protein (CAB60261.1|
                     MrsM protein [Bacillus sp. HIL-Y85/54728]; CAD60521.1| CinM
                     protein [Streptomyces cinnamoneus]). COULD BE IMPLICATED IN
                     THE PROCESSING OR THE EXPORT PROCESS OF THE LANTIBIOTIC
                     produced by Cmm_fin_1764. pfam05500, NisC,NisC-like family.
                     Lantibiotics are peptide-derived, post-translationally
                     modified antimicrobials produced by several bacterial
                     strains.; Specificity unclear"
                     /product="putative lantibiotic modifying enzyme"
                     /protein_id="CAN02024.1"
                     /sec_met_domain="LANC_like (E-value: 9.8e-47, bitscore:
                     148.2, seeds: 47, tool: rule-based-clusters)"
                     /sec_met_domain="DUF4135 (E-value: 1.4e-92, bitscore:
                     299.6, seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITAAELSLLQASRLRERRAATRADARPVPELTRAWRDDALVDD
                     GTHAAYLAGQLLDGDDLVALLAGDAWTPRAASADWLARVDPWLVHPPVSAEAYATANGH
                     LVVEDTRVAGLPFAALLGPMVGAQRAHLDGRPWLSRTATADLLDGLAERLATLSLRALL
                     AELARDPAPGRHARLDARLADPEHRAELLSRHPVLARDLVTAIGSWRAHVARILDRLHA
                     DHGDLVRMGLATERLDALEAVDLSSGDPHDGGQSVATLRFSDGSLLVYKPRDCRVFALY
                     RDLVDALAPALPGEIRLRAAAVLPRDGYGWVEFVAHAEDAASAIPLSRHLGRLGSLLAV
                     AHVLGASDLHLENVIASADGPVPIDLETLIQNRSEVDGEDGTALRRASRMLNASVLGSG
                     ILPVQLTTGEGTSIDVSVSTGGLHGAGTTATVHQVVDAATDRMRIEAREMPVGRSRNQP
                     PGATLARIRSGRAALADGYERTFRAIVERRAEVRAILSAAPDISSRHIVRATRSYSLLL
                     TEMRRPRPLRSGIDRDHLLRHLWTRVEEHPADAALVEAEERALWRLDVPLFSTRMDARA
                     LIADDEEAGPQRFARSTREDVLDRLARLTLDDLPESLRLVDESVLAATATPDGDDDARD
                     DARGRQAADATASLARAPRVDDDALREAARGQARILLASAVLGRDDATWIGVSSSLDSS
                     GLEYRPAGPTLYDGLAGIAFAATHAHGVLPGLGLDDLAGRAMHAVTAILDDWTRGLIDL
                     PVGAYSGATGLLHALAHHDARVGGDGHRDLRAAAVRRLGEVAHEDPHLDVMAGVAGACA
                     VVAGLPEGATEAGRHALRALAGRLMSTAVEVEGGALVWETGAERARLGGFSHGASGVGW
                     ALARTAGVLGDDAIADAAVRALRFDDALFDPSRRRWRDARPEARSAAGSFPAHWCHGTA
                     GIAMARADAAATLDRPDLLELALVGARETASDALPSDDSLCHGTLGNLIAVRGATRRTG
                     VDTGLAEFRDRALQRVLRAGPRSGLPQGVTSVRGLMLGTAGALYALCRELEPGIPDALL
                     LEGPR"
ORIGIN
        1 tcagcggagc ttgaccacgc ggatagccgc gagggtgagg aggacgacga gcgcgccgag
       61 gcccacgctc gtcgcgagga gcggcagggt gtccgccgtc gtgaggcccc cggatcccgc
      121 gatcgccgtc gaccccaggt tcagcgcccg ggtgttgatc gcctgcggga gcacctccgc
      181 cagcggacgc agggccgccg aggtcacggc ggcgacgccc gcgacgcacc cgacgaggca
      241 cagcgcgacc ggcgccgcga aggaccgcag gagcagcgac agcagggact ggacggcgat
      301 gaggggcagc gccgccacgg tcgcgatcag ccccacgagg aggaacgcga cggggaacgg
      361 gccgtccagc cggagcacga acagcccgga caccgccgtg ccgccgacga gcaccgcctg
      421 catcagcgcc accggcacga ggatcgccac gatcttcgcc agcacgagtc ggatcggtcg
      481 cgcggtcgcc gtcagcaggg cgttccagtt ggtgccgcgg tgctcgacgc gccagatggc
      541 cgccgcgagc aggctgatgc ccatcgagta gaagagcagg ccgtagaaca gcaccacctg
      601 cgaggtgaag gacgcccacc cggagtccag ggtctgcgtg ttgttcgcca ggttgatggt
      661 gccggtgacg acggccagga gcggcagcag caccgcgatg atccagatgc tggagcgctt
      721 gagcttcacg gcctcggctc ggaacacgcc cgtcacctct ccacgcggtc gagccggatc
      781 gtcgcgagcg cgaaggccgc cgcgaccacg acgaggaagc cggcgatcca cccgagcggc
      841 gaggcgacgt agcccacgcc cgcgtccgac tggccggcct gcgagatgac ggcgtagtac
      901 ccccacggga tggcccggct cgcgacggac gggagcagga gggagaagac ggccaggaag
      961 gcgcccagca tccccacgcc cacgctgatg agctggttct cgacgcgcgc cgcgagccag
     1021 acgtgcagcg cgcagaacgc cacgtcgacc aggtagagca ggagcgtgta cagcgcccac
     1081 ggccccacct cgacgggcac ccggatcccg gcggcgtacc cggcggcgat cacgagcagc
     1141 gattggatca gcacggcggg cacgaggagc accgcgaggg ccacgagctt ggcccggcac
     1201 agctcccccg ccgtgcgtcc cgcgcccgcc gcgaggatcc acccgcccgc ctggtgctcg
     1261 atgtcggtct ggcggctcgc gatcaccgcg gtgaggatcg gcgaggtcat cgccgccatg
     1321 agcgtgtagc cgagcaggag cgcggcccag gggtgcgcgc ccggatcgtc gaagcccgca
     1381 cgcgtgctgc ccgcgaactg cgacgccgag ctcagggcgg cgacggccac caccatgccg
     1441 atgagcagcg ggagcgagcg gagccggtgc atcttgcgga actcgatcgc cacggcgcgc
     1501 ctcacagctg ccccccgcgc ccggtgatgt ccatgaacac gtcctccagg ctctgcggca
     1561 gccgccgcac ctcgtgcacg ggcacgtcgg ccgccacgag ccggcgcacg atgtccgcgg
     1621 tctcgtcctt ggagcgggcg ctgagccgga tcccctccgg gctcctggcc gccccggcga
     1681 cctccgccat ggcccggtcc ggcgcggacg tctcgatgac gaggtcgggg atggagtgct
     1741 cgaacagctc ggcgcgcgtg ccctggaaga tcatccggcc gtcggccagg atgccgagca
     1801 cgcccgccat cttctcgatc tcgtcgagga ggtggctgga gaccatcacg gtgatgccct
     1861 ggccggccag gtccatgagg agggagcgga cctcctcgat gcccgccgga tccagcccgt
     1921 tggtgggctc gtcgaggacg agcagcgagg ggtcgcgggc gagggccatg gcgatcccca
     1981 gccgctgctt cattccgagc gagtaccggc gcaccagctt gtcgcggtgc tgcgtgagcc
     2041 gcacggtcgc gaacgcccgc tccacctggg cctccgcgag cccgagcatg tcgcgcacga
     2101 tccgcatgtt ctcccacccc gtgaggtggc cgtagccggc gggcgcctcg atcatggagc
     2161 cgatggacgg caggagcccg ccgcgcgtcg ccggcgtgag ccgctggccg aacaggtgga
     2221 tctcgcccga cgtgggctgc gtgagcccga gcagcatctt catggtcgtg gacttgccgg
     2281 agccgttggg cccgaggaac ccgtacacgg acccctgcgg cacctcgatg tcgagcgcgt
     2341 cgacggcgac gtgatcccgg aaccgcttgg tgagcccgcg ggtggcgatg acggcgctca
     2401 tcggcgcggg ggtgtcgtgg tcggcatgcc cccatactcg gccggccgcc gcgcgggcgc
     2461 atcgtgccgt gggccggtcc cgcccggtcc cctctcctgc gatgggagga gcgggtggca
     2521 ggtcggcggg accgcgcccc atgcgccgca gcggtcaggc ggcgaggccc cgccggtgcg
     2581 cccagatcac caggtggacg cggtcgcgca cggcgagctt ggtgaggatg tgcccgacgt
     2641 gcgtcttcac cgtgacctcc gcgatggaca ggtcgcgggc gatctccggg ttggacgcgc
     2701 ccgtgacgag gcgcgtgaac acgacccgct cccggtcggt caggacgtcg agcgctgcgg
     2761 tgtccgggtc gggcggcgcc gtcctctcga cggccagccg ctcgaggagc cgctgcgtgg
     2821 tgctcatcga ggcggcgaac gtgccgttgt gcacggaccg gatcgcgtcg accagctgca
     2881 cggcggtgct gtccttcagc aggaagccga cggcgccggc gcgcagcatc cggatggcgt
     2941 aggagtccgt gtcgtgggtg gtgagcccca ggatccgcgt cccctcggcg cgcccgccct
     3001 ccacgagccg gcgggtcgcc tcgatgccgt cgaggaccgg catgcggacg tccatgagca
     3061 ccacgtccgg gcggagctcg acgacgcggg cgacggcctg ctcgccgtcc gcgcagacgc
     3121 cgaccgcggc catgtccggc tgcgcgtcca cgatgcgggc gaagccgtcc cgcacgaggg
     3181 actggtcgtc gaccacgacg acccggatcg gcgcgggcgc gtcggtcacg gggtgctccc
     3241 gccgagcggc gcggtcggcc accgcgcctg gaggacgaac tcgtcgtcgc acgcgtaccg
     3301 ggcctgccct ccgtagagcg cgcagcgacg gcggatcgcg gcgaggcccc ggtgctcctg
     3361 cacggggcat ccccgcttgt cgcgaaccac ggcattggcg acggtgatcg ccagcgcgtc
     3421 gggcgaccac tcgacgcgga ggcgcgcggt gccgcccgcg ccgtgccgca tggcgttcgt
     3481 gagggactcc tgcacgaccc ggtagacggc gaagcccacg cccggcagga ggggtcgcgg
     3541 cgagcccgcg tcctgcctgt cgatgccgag gccggagccg cggaagccct cgacgagggc
     3601 gtcgacgtcg tcccagccgg ggatgggacg cacgtccgcg tcggcgtcgg tgggcgcgag
     3661 gagcccccgc acctcgtgca gggcggcccg gctggtctcg gagatggtgc ggaacatgcg
     3721 cgacgcctcg gggctcggcg cggtcgcccg gccgctctcc gcctggacga tgacgctggt
     3781 cagagtgtgc gccagcacgt cgtgcatgtc gtcgctgatg cgctcgcgct cctcgtaggc
     3841 ggcgagccgc gtccgggcgt ccgcgtcgcg ttcggacagc gtcgtccgga gctcggcggt
     3901 gcggacggcc tcgctgcggc gccgttcgag cagaccggcc gcccatgcga ccgcgaagct
     3961 cacggacacg agcgcgatga ccatgccgac ggatcccgtg gagcccagct ccccgccggt
     4021 gagggtgcgc gcggcgagga cgaccacgaa gcacgcgccg agggcgaggc cgacgtgccg
     4081 gagccggtcc gcggcgtggg cggcgacgga gtaggccgcc accaggagca cgacgtcacc
     4141 cgggaacagg agcgagctcg tggcgagcat gtgcgccgac gagatcgccg tggccagcag
     4201 cgcggcggtg acgggccgcg cgtggcgcac cgcgagcgtg agcgccatga gggacgacca
     4261 gatccagtcg ggctggccca ggacgcccat gatggcgcac aggacgacgg cagcgcccgc
     4321 gacgacctgg tcgccttgcg cgcgcagact cccccgtcgc atcacgtcct caggctagcg
     4381 gggaggacac gcccggccgc cggcggacgc cctccccccg gccccccgtc cgcggggcat
     4441 cccggtcgcg gcctccgacg atgggcggag ggcacggccg cggatgtcat cgggtcgcac
     4501 gatgcgctcg gacggaagcc gctgggaacg tgaacaccag ggatcgcgca tgggctgcgc
     4561 gtcgacgccg caccggccga ggcgcacgga tccgcgatcc ccacggggca agtcggacct
     4621 gtgacggccc atgggccatc gggatgggtg cggctcgcac cacgacggtc tgggccgacc
     4681 ggccgggatc catgcgcgac aacaggaaca caggtggaac acgatgaacg acatcctcga
     4741 gacggagacc cccgtcatgg tcagcccccg gtgggacatg ctgctcgacg cgggcgagga
     4801 caccagcccg tccgtccaga cccagatcga cgcggagttc cgtcgcgtcg tgagcccgta
     4861 catgtccagc agcggctggc tctgcacgct caccatcgaa tgcgggacga tcatctgcgc
     4921 gtgtcgctga cggacgccct cgacggacgg gctgcgctcg gcgcgcggtc cgtccgctcc
     4981 gcgtcgggcc ccgcccgccg gagcggcgag ggggatccga cgtgacgatc accgcggccg
     5041 agctctcgct gctgcaggcg tcccggctgc gcgaacggcg cgcggcgacg cgggccgatg
     5101 cccgaccggt ccccgagctg acgcgcgcct ggcgcgacga cgccctcgtc gacgacggga
     5161 cgcacgccgc ctacctcgcc gggcagctgc tggacggcga cgacctcgtg gcgctcctgg
     5221 cgggcgacgc gtggaccccg cgggccgcga gcgcggactg gctcgcccgg gtcgacccct
     5281 ggctcgtgca tccgcctgtc tccgcggagg cctacgcgac ggcgaacggc cacctcgtcg
     5341 tcgaggacac ccgcgtggcc gggctcccct tcgccgccct gctcggcccg atggtcggcg
     5401 cgcagcgcgc gcacctggac ggtcgcccgt ggctctcgcg caccgcgacc gcggatctcc
     5461 tcgacggcct cgcggagcgc ctcgcgaccc tctccctccg ggccctcctc gcggagctcg
     5521 cccgggatcc ggcaccgggt cggcacgcgc ggctggatgc gcggctcgcc gaccccgagc
     5581 accgcgccga gctcctctcc cgccaccccg tcctcgcgcg cgacctcgtc acggcgatcg
     5641 gcagctggcg cgcgcacgtc gcgcgcatcc tcgaccgcct gcacgccgac cacggcgacc
     5701 tcgtccggat gggcctcgcg accgagcggc tcgacgccct ggaggccgtg gacctgagct
     5761 cgggcgaccc ccacgacggc gggcagtccg tcgcgacgct gcgcttctcc gacggcagcc
     5821 tgctcgtcta caagccgcgc gactgccggg tgttcgcgct gtaccgcgac ctcgtcgacg
     5881 cgctcgcgcc cgccctcccc ggcgagatcc gcctgcgcgc ggccgcggtg ctgccgcgcg
     5941 acgggtacgg ctgggtcgag ttcgtggccc acgccgagga cgccgcctcc gcgatccccc
     6001 tgtcccggca cctcggccgg ctcggctcgc tgctcgccgt cgcgcacgtg ctcggcgcgt
     6061 ccgacctcca cctcgagaac gtcatcgcct ccgcggacgg tcccgtgccc atcgacctgg
     6121 agacgctgat ccagaaccgc agcgaggtcg acggcgagga cgggacggcc ctgcgccggg
     6181 cgtcgcggat gctcaacgcg tcggtgctgg gcagcggcat cctcccggtc cagctcacca
     6241 ccggggaggg cacgagcatc gacgtcagcg tcagcaccgg gggcctgcac ggcgcgggga
     6301 ccacggcgac cgtccaccag gtggtggacg cggccacgga ccgcatgcgc atcgaggcgc
     6361 gcgagatgcc ggtcggccgg tcgcgcaacc agccgccggg agcgacgctc gcgcgcatcc
     6421 gctccggtcg cgccgccctc gcggacgggt acgagcgcac cttccgcgcc atcgtcgagc
     6481 gccgggcgga ggtccgcgcg atcctgtcgg ccgcgcccga catctcgtcg cggcacatcg
     6541 tgcgcgccac ccgctcctac tccctgctcc tcacggagat gcgccgaccc cgcccgctcc
     6601 gcagcggcat cgaccgggac cacctcctcc gccacctctg gacgcgcgtc gaggagcatc
     6661 ccgcggatgc cgcgctggtc gaggcggagg agcgcgccct ctggcggctc gacgtgcccc
     6721 tgttctccac gcgcatggac gcacgcgccc tcatcgccga cgacgaggag gccgggccgc
     6781 agcgcttcgc gcggagcacg cgcgaggacg tgctcgaccg gctcgcgcgg ctcaccctcg
     6841 acgacctccc cgagtccctc cggctggtcg acgagtccgt gctggcggcg accgcgacgc
     6901 ccgacggaga cgacgacgcc cgcgacgacg cgcgaggacg gcaggccgcg gacgccaccg
     6961 cctcgctggc ccgcgcaccg cgggtggacg acgacgccct gcgggaggcg gcacgcggac
     7021 aggcccggat cctcctcgcc tccgccgtcc tcggccggga cgacgcgacc tggatcggcg
     7081 tgagcagcag cctcgacagc agcggcctcg agtaccgccc cgcggggccc accctgtacg
     7141 acgggctggc cgggatcgcg ttcgccgcga cccacgcgca cggcgtgctg cccggcctcg
     7201 ggctcgacga cctggccggc cgggcgatgc acgcggtcac ggcgatcctc gacgactgga
     7261 cgcgcggcct gatcgacctg ccggtgggcg cctacagcgg cgcgaccggg ctcctccacg
     7321 cgctcgccca ccacgacgcg cgagtcggcg gagacgggca ccgcgacctc cgggcggcgg
     7381 ccgtccgacg gctcggcgag gtggcccacg aggatccgca cctggacgtg atggcgggcg
     7441 tcgccggcgc gtgcgccgtc gtcgcggggc tgcccgaggg cgcgaccgag gcgggccgcc
     7501 atgcgctgcg cgcgctcgcg gggcggctga tgagcacggc ggtcgaggtg gagggcggcg
     7561 ccctcgtctg ggagaccggt gccgagcggg cgcggttggg cgggttctcg cacggcgcat
     7621 ccggcgtcgg ctgggcgctc gcccgcaccg ccggcgtgct gggcgacgat gccatcgccg
     7681 atgcggccgt ccgcgccctg cgcttcgacg acgcgctgtt cgacccgtcc cgccgccgct
     7741 ggcgcgacgc gagaccggag gcgcgatccg cggccggatc cttccccgcg cactggtgcc
     7801 acggcaccgc cggcatcgcg atggcacgcg cggacgcggc cgcgacgctc gaccggccgg
     7861 acctcctcga gctcgccctc gtcggcgcgc gcgagacggc gtcggacgcg ctcccctccg
     7921 acgactccct gtgccacggg accctcggca acctcatcgc cgtgcgcggc gcgacccgac
     7981 gcaccggcgt cgacaccggg ctcgcggagt tccgcgaccg cgcgctgcag cgcgtcctcc
     8041 gcgccggccc gcgcagcggt ctgccccaag gggtgaccag cgtgcggggg ctcatgctcg
     8101 gcacggccgg cgcgctctac gcgctgtgcc gggaactgga gccggggatc ccggacgcgc
     8161 tgctgctgga ggggccgcgc tga
//
