LOCUS       BGC0000530             10118 bp    DNA     linear   BCT 11-FEB-2013
DEFINITION  Streptococcus mutans N66 contig14, whole genome shotgun sequence.
ACCESSION   BGC0000530
VERSION     BGC0000530
KEYWORDS    .
SOURCE      Streptococcus mutans N66
  ORGANISM  Streptococcus mutans N66
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
COMMENT     Annotation was added by the NCBI Prokaryotic Genomes Automatic
            Annotation Pipeline Group. Information about the Pipeline can be
            found here:
            http://www.ncbi.nlm.nih.gov/genomes/static/Pipeline.html. Please be
            aware that the annotation is done automatically with little or no
            manual curation.
            ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 13:47:39
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 10118
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..852
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR01716"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="SMU76_03150"
                     /note="COG1396 Predicted transcriptional regulators"
                     /product="hypothetical protein"
                     /protein_id="EMC15120.1"
                     /sec_met_domain="TIGR01716 (E-value: 1.8e-65, bitscore:
                     209.8, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELGELYRELRIARGLKIKDIACDNLSKSQLSRFENGQTMLAADK
                     LLLAISGIHMSFSEFGYALSHYEESDFFKIGHKLSELYVRQDIEGLKKLLEINDEYEVF
                     DVYNRLNKLVIKVSIHSLDADYIISDDDKNFLTTYLYSIEEWTEYELYIFGNTMSILSS
                     DDLIFLGKAFVERDKLYIYLPSHKKNAELVFLNLILLLIERKKLYQAIYFVENLEKLLN
                     YQDMFATTFLKFFKKIIAYLQEKSIDISELERYVDIVGEINPTIATILKSNLNQLLSNC
                     NH"
     protocluster    1..10118
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[2321:6555]"
                     /cutoff="20000"
                     /detection_rule="((LANC_like and (Lant_dehydr_N or
                     Lant_dehydr_C)) and not (YcaO or TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      2322..6555
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="((LANC_like and (Lant_dehydr_N or
                     Lant_dehydr_C)) and not (YcaO or TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
     cand_cluster    1..10118
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((LANC_like and (Lant_dehydr_N or
                     Lant_dehydr_C)) and not (YcaO or TIGR03604))"
                     /kind="single"
                     /product="lanthipeptide-class-i"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..10118
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000530"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..10118
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lanthipeptide-class-i"
                     /region_number="1"
                     /rules="((LANC_like and (Lant_dehydr_N or Lant_dehydr_C))
                     and not (YcaO or TIGR03604))"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            1..852
                     /locus_tag="SMU76_03150"
     gene            1187..1390
                     /locus_tag="SMU76_03155"
     CDS             1187..1390
                     /codon_start=1
                     /locus_tag="SMU76_03155"
                     /product="hypothetical protein"
                     /protein_id="EMC15121.1"
                     /transl_table=11
                     /translation="MGNQLEINIDGEKKCVKKEASKGSFCFEQLCCDNFLLKHGNDGYN
                     DNLFSFIIRVVIREVANKESIK"
     gene            1712..1903
                     /locus_tag="SMU76_03160"
     CDS             1712..1903
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gallidermin"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) mature_b"
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="SMU76_03160"
                     /note="COG1012 NAD-dependent aldehyde dehydrogenases"
                     /product="hypothetical protein"
                     /protein_id="EMC15122.1"
                     /sec_met_domain="Gallidermin (E-value: 3.1e-32, bitscore:
                     100.1, seeds: 3, tool: rule-based-clusters)"
                     /sec_met_domain="mature_b (E-value: 1.8e-15, bitscore:
                     45.7, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSNTQLLEVLGTETFDVQENLFTFDTTDTIVAESNDDPDTRFKSW
                     SFCTPGCAKTGSFNSYCC"
     CDS_motif       1712..1834
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence:
                     MSNTQLLEVLGTETFDVQENLFTFDTTDTIVAESNDDPDTR"
                     /locus_tag="SMU76_03160_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       1835..1903
                     /prepeptide="core"
                     /locus_tag="SMU76_03160_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class I"
                     /score="19.00"
                     /molecular_weight="2270.6"
                     /monoisotopic_mass="2269.0"
                     /core_sequence="FKSWSFCTPGCAKTGSFNSYCC"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MSNTQLLEVLGTETFDVQENLFTFDTTDTIVAESNDDPDTR
                     "
                     /leader_location="[1711:1834](+)"
                     /alternative_weights="2288.7"
                     /alternative_weights="2306.7"
     gene            1973..2167
                     /locus_tag="SMU76_03165"
     CDS             1973..2167
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR03731"
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="SMU76_03165"
                     /product="hypothetical protein"
                     /protein_id="EMC15123.1"
                     /sec_met_domain="TIGR03731 (E-value: 3.1e-21, bitscore:
                     64.5, seeds: 23, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLNTQLLELFLSDTLDVREDLFDLNITDTAVIQSNDSPDTRSRVG
                     SHSICPPRKTSASFNSYCC"
     CDS_motif       1973..2095
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence:
                     MLNTQLLELFLSDTLDVREDLFDLNITDTAVIQSNDSPDTR"
                     /locus_tag="SMU76_03165_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       2096..2167
                     /prepeptide="core"
                     /locus_tag="SMU76_03165_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class I"
                     /score="16.90"
                     /molecular_weight="2315.7"
                     /monoisotopic_mass="2314.1"
                     /core_sequence="SRVGSHSICPPRKTSASFNSYCC"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MLNTQLLELFLSDTLDVREDLFDLNITDTAVIQSNDSPDTR
                     "
                     /leader_location="[1972:2095](+)"
                     /alternative_weights="2333.7"
                     /alternative_weights="2351.7"
                     /alternative_weights="2369.7"
                     /alternative_weights="2387.7"
     gene            2322..5294
                     /locus_tag="SMU76_03170"
     CDS             2322..5294
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_N"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lanthipeptide_LanB_RRE"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1155:Lantibiotic dehydratase domain protein (Score:
                     655; E-value: 2.9e-198)"
                     /gene_kind="biosynthetic"
                     /locus_tag="SMU76_03170"
                     /product="hypothetical protein"
                     /protein_id="EMC15124.1"
                     /sec_met_domain="Lant_dehydr_N (E-value: 2.8e-120,
                     bitscore: 391.5, seeds: 73, tool: rule-based-clusters)"
                     /sec_met_domain="Lanthipeptide_LanB_RRE (E-value: 1.7e-12,
                     bitscore: 37.3, seeds: 20, tool: rule-based-clusters)"
                     /sec_met_domain="Lant_dehydr_C (E-value: 7.6e-19, bitscore:
                     57.5, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNDFQFQDYFMYRKPLGNFSNFLSITDTTDPIELLHSDPIFAEGV
                     YLASSSLRAAINKLENHTASAKDKKNAKETIFQYYARYNTRSTPFGLFSSIGVGAFSAQ
                     PKQEKSRYEKSVNIDLFWAYKVAHKLENMPNVLNTLKVVANNALQKSDNFWLLDTRSCF
                     GLMNSRSDIHEDITVKSNQLIDYVINCADQPIYYPTLVNNIAEKFSQSSDDVKKYLQKL
                     IKEEFLITELKFSLIEDNPLDWFINILEKTQNNLELLEKLIEIKAMIQDYTDCDIGEGN
                     NLILALENKMSQIAEANAYLRVDLYDHAELELSHHIKSSLQNILKVLSSFSPGINSRKG
                     IRNYHEKFISRYGYEQLVPLQLLLNSTSGLGFPKEYSQTKDFKQNNGDSRNQKITEFLQ
                     RKFEKSLRDGKEIILKDDDLKELDFDREQQISGELYCFYDFKNKKLEVSGLGVSPMIGD
                     TFGRFHAKLPNTIVTENVNKTKEIFAKAYPDAIITQLTEVPYFGRSANVMVSNSLKSHQ
                     LELRNYASKKDMSINDIYVGATSEELYFYSKKYGKRVIFIMNSMFNYINGSKLLRFLLE
                     VSNSDFQNITPIMFGNLDSYNRVPAITYKDIIIKPETWNIRKSEVKTLDSLKNWLSDND
                     VPSLVRMKYADQIIYLDLSRTIDLTMLFRSIKKHSSIQLLATHSKFVNDMKVLELVVPF
                     TRSGVNARQIYHYAQNIYTLEDSDSKEKYFYVKIYVNKQRQASFLQKEYPLLLNYLKLP
                     ENLQWFYIRYEDDEKDSIRFRIRYVEDKQLIQLYSRFIEWATEARKNIQISGYEISEYI
                     PESARYGGKKYSSIIHSFFHYDSILSLLLQKKTDQAIEVRTSLSIIRMFLMMKLNLQDQ
                     QELMKNLFDGEHKLKYEKEYHNSISLLLDSLCIRNQIDEADTFCVMNMKIITEEVSSVL
                     KQEDLTADWQRILGSLIHMRCNRVYGINSELERKTMFIVDKVINSKRYTDMFLGVDNDT
                     K"
     gene            5281..6555
                     /locus_tag="SMU76_03175"
     CDS             5281..6555
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: LANC_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1140:Lanthionine synthetase C family protein (Score:
                     272.9; E-value: 7.2e-83)"
                     /gene_kind="biosynthetic"
                     /locus_tag="SMU76_03175"
                     /product="lantibiotic epidermin biosynthesis protein EpiC"
                     /protein_id="EMC15125.1"
                     /sec_met_domain="LANC_like (E-value: 3.9e-56, bitscore:
                     179.5, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIQSKRVEKIKNLLTEQTYLFDYQEVLKKNSQVKQTDFWNLLSLS
                     SGITSLLIFYQEYENLEKIDLKQQKQSLIGLISHYVNQIADKSSLFDGLAGVGFAINYV
                     SNNGKYYQELLNQIDSKVRQNVKRNLAVCNSEEYTNPTNYDVISGNAGVARYLMERESS
                     ENWQIVKMILETFYKALEQGWRVQPKYQFLEAEKQYYSKGNINFGLAHGVLGPTTIMSL
                     YQQRYPQDTRNAEKLQETYQLIKRYAQVRDEGLRWPIRYDLLRKEASFILRNGWCYGDN
                     GVYNTLFLIGKVLSNQEICETARKVIPSIIRDDYEKMESPTFCHGFAGKANFFLLQYQR
                     TNESIFLVKAEEEIDKILVMYNSENMFGFKDIEDNIDNTGERLTYWDNFGLLSGTVGIL
                     LVLMEYCNIVNAGKVAEWNKIFLLT"
     gene            6578..7144
                     /locus_tag="SMU76_03180"
     CDS             6578..7144
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Flavoprotein"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="SMU76_03180"
                     /note="COG0452 Phosphopantothenoylcysteine
                     synthetase/decarboxylase"
                     /product="lantibiotic biosynthesis protein, flavoprotein"
                     /protein_id="EMC15126.1"
                     /sec_met_domain="Flavoprotein (E-value: 1.4e-35, bitscore:
                     111.4, seeds: 143, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEEQNTEKKILLCLTGSGALLGIAEYITFLTVRFKHVRVIVSDNA
                     AKMLPVAAITQLCEKVYTDEVSFIDKQKNHIALTRWADITVVLPATANIIGKVANGIAD
                     NFMTTTLLSSSKPVLIYPCMNNIMWENPVVQKNVETLTGTQYKVIVGQESESFELASGK
                     MKKNIAIPSLEELQQVILENLQEER"
     gene            7149..8492
                     /locus_tag="SMU76_03185"
     CDS             7149..8492
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075:alkaline serine protease, subtilase family
                     (Score: 235.8; E-value: 1.9e-71)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="SMU76_03185"
                     /note="COG1404 Subtilisin-like serine proteases"
                     /product="peptidase, S8A family protein"
                     /protein_id="EMC15127.1"
                     /sec_met_domain="Peptidase_S8 (E-value: 1.5e-44, bitscore:
                     141.6, seeds: 43, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKKGLLVIIFLTFFFFYPKVKAAEYTIISNNSEQTVNDLNNLGV
                     TVNNHIAEIGYIEAQGDVNIDQIKKLSNIQSIQNMADTSQNITTRVPSTYINQTIQLPQ
                     LFSYQWDMQKITNNGVSYSLNKGNRKNVTVALVDSGIDVEHNAFTGMIDSRSKNFVPAG
                     GYENSENSETGNISNIDDKKGHGTAIAGQIAANGQILGVSPGTNLLVYRVFGKSKSKEC
                     WILKAIIDATNNGANVINLSLGQYIKIPNGNIWESAEALGYKFAIDYATRHNAIVVAAT
                     GNDGLSEDNGEIKTYYNNQHSGQDTSQNYTVEDYPSVLPNTIAVGSSDNNNQRSSFSNY
                     YNQSHSNFILAPGGGTTLLDKYGREEWYNQKLFMKEQVLSTSNNGNYDYVDGTSVSTGK
                     VSGELAEIISNYHLQRNSPKARRILLNQVNYTKDGYKEISVYKALRGH"
     gene            8493..10118
                     /locus_tag="SMU76_03190"
     CDS             8493..10118
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288:ABC
                     transporter related protein (Score: 229.1; E-value:
                     1.7e-69)"
                     /gene_kind="transport"
                     /locus_tag="SMU76_03190"
                     /note="COG1132 ABC-type multidrug transport system, ATPase
                     and permease components"
                     /product="ABC transporter, ATP-binding and permease
                     protein"
                     /protein_id="EMC15128.1"
                     /transl_table=11
                     /translation="MKWLEVLQISKKERILYLIGCIFSILTGLITLQITYLLKNLVDNK
                     SSFNNLFLFLVLGLVLFIIDTGSQYLISLIGNQVVFNSRNNIWRKISDWTDSKDDSSEM
                     AGHLINDSELIENFIISTIPQTVNSVIVGSGSLVMLFIINSKMSIEVIGICLLLLFIMQ
                     PFSRILGKISKKIQEDKAELINVTSQLRGQVKTIKSYNAQDYALQKFDEKNRKLFQDIL
                     NRIKVFSVYSPFLNILVLFMIITVVWLGNAEVRSGNLTVGSATIFVVYMTQLINPIMQL
                     SQLVAHIGMLNGGVERLLVYNQAVPEKSGIKKIDEIIDIVFDNISFAYDDQKSIIENVN
                     LTFQKGNYISIVGESGVGKSTLLDLLEHNYTPLKGQILINGINLEELDIKTFRNKISYV
                     SQEPAILSGTIRELLDFNQQQHTEANLWNVLDIVELTELIRNLPQKLDSKIDEYGGNLS
                     GGQMQRISLARGLLKKGDVLLLDESFANIDEETCLRIKSKIAAYAQSHKQIVIEVIHNL
                     NRITPNSIVYQLVDKKLEILRRGF"
ORIGIN
        1 atggaattag gtgaacttta tcgagaattg agaattgcta gaggtttaaa gataaaagat
       61 atagcttgtg ataatttatc caagtcacag ctatctagat ttgaaaatgg acaaaccatg
      121 ctggcagctg ataaattatt gttggctatt tcagggattc atatgagttt ttcagaattt
      181 ggttatgctt taagtcatta tgaggagagt gattttttta aaataggtca taaattgtca
      241 gaattatatg ttcggcaaga tattgaagga ttaaaaaagt tgttggaaat taatgatgag
      301 tatgaagtat ttgatgttta taatcgttta aataaattgg tgattaaagt ctctattcat
      361 tcactagatg ctgactacat aatatcggac gatgataaga attttttaac aacttatcta
      421 tacagtattg aagagtggac tgaatatgaa ctttatatct ttggaaatac tatgtctata
      481 ctatcatctg acgatttaat ttttttgggg aaagcttttg tagaacgtga taaattatat
      541 atatatcttc ccagccataa gaaaaatgca gagttagttt ttttaaattt aattttactt
      601 ttaattgaaa gaaaaaaact ttatcaagca atttattttg tagagaattt agaaaaatta
      661 ttaaattatc aagatatgtt tgcaacgaca tttttaaaat tttttaaaaa aattattgct
      721 taccttcaag agaaatcaat agatatatct gaattagaac gttatgttga tatagttgga
      781 gaaataaatc ctactattgc aactattctt aagtctaatt tgaaccagct tttatcaaat
      841 tgtaaccatt aaaaatattt taatagattt cgtatgttcc ggtatttgtt aaatttagag
      901 taagtggatc ataaattctg ataaaaagtg atggaacaat aactccattg cctcaggtag
      961 aatacaaagg tcttagaatt cattttctga gatctttttt gatgttttgc agttttttta
     1021 aagggaaatt gttgactagg tgttgttgta cttttttggt attttctctt tataccaaat
     1081 tatcaaaaac attgatatta atgtagttta ctcttgtttc tagaatttac gattactttg
     1141 gggaatatgg ctttccaatt agctttctga tcaataagct cagtttatgg gtaaccagct
     1201 tgagattaat atagatgggg agaaaaaatg tgtaaaaaaa gaagcttcaa aaggaagttt
     1261 ttgttttgaa cagctatgtt gtgataactt tcttttgaag catggtaatg atggatataa
     1321 tgataattta ttctctttta ttataagagt ggtaataaga gaagtggcaa acaaagaatc
     1381 aataaaataa tacgtttaac cataatattt cctcctttag ttcattataa cagtaaaaga
     1441 ggaaatatct atatatttgc aaatatggga taaataaata taaaaaaaag caaatttgca
     1501 atttttaaaa aataagggat aacagtgata ttatattatt gtaaatatat atttacttgg
     1561 taatagtggt ttgctattgt aaaatttttt gctattatct tacaattata attgtatatc
     1621 ctactatcag atcacagcta aatgtttttt ctggtatgct ttcactgggc aatctatgtt
     1681 cagttacaca ttatgataaa aggagaatgt tatgtcaaac acacaattat tagaagtcct
     1741 tggtactgaa acttttgatg ttcaagaaaa tctctttact tttgatacaa cagatactat
     1801 tgttgcagaa agcaatgatg atccagatac tcgtttcaaa agttggagct tttgtacgcc
     1861 tggttgtgca aagacaggta gtttcaatag ttactgttgt taattgtata aaagatttag
     1921 gttgtgccgc atgttagcgg cacaatcttt tgatattgga gaggtattaa atatgttaaa
     1981 tacacaatta ttagaattat ttttatccga tactttagat gttcgagaag atttatttga
     2041 tctcaatata acggatactg ctgtaataca gagtaatgat agtccagata ctcgtagtag
     2101 agtcggtagt cacagtatat gtcctcctcg aaagacttcc gccagtttca atagttactg
     2161 ttgttaacta taaattatac ttaaattgat agtaaacttg gtcatgacat gatcatatgt
     2221 tgatattgga agagaatcaa acttataaaa acaattaaat ctaaatttaa tgaatattta
     2281 gatgaattgc tactaagtta atagtcatgt taggagaaga gatgaacgat tttcaatttc
     2341 aagattattt tatgtacaga aaaccattag gcaacttttc taattttctt agtataactg
     2401 atacgacgga tcctattgag ttactacata gtgacccgat atttgctgaa ggagtatatt
     2461 tggcctcttc gtctcttaga gcagctataa ataaacttga aaatcatact gcgagtgcta
     2521 aggataaaaa gaatgcaaaa gagactattt ttcaatacta tgcccgttat aacacgagat
     2581 caactccatt tggcttgttt tcgtccatcg gagtaggtgc tttttcggct caacctaaac
     2641 aagagaagtc tcgctatgaa aaatctgtta atattgatct tttttgggct tataaagtag
     2701 cacataaact agaaaatatg cccaatgttt taaacacttt aaaagtagtt gctaataatg
     2761 ctttgcaaaa atcagataat ttttggcttt tggatacgcg aagttgtttt gggcttatga
     2821 attcacgttc tgacattcat gaggacatca cagttaaatc taatcagctt atagattacg
     2881 ttattaattg tgcagaccaa cccatttact atccaacatt agtcaataat attgccgaga
     2941 aattttctca gtctagtgat gatgtaaaaa aatatttaca aaaattaatc aaagaagagt
     3001 ttttgataac tgagttaaag tttagtttga ttgaagacaa ccctttggat tggtttatta
     3061 atattttaga aaaaactcaa aataacctag agttacttga aaagttgatt gagataaagg
     3121 caatgattca ggactatact gactgtgaca ttggtgaggg caacaatttg attttagctt
     3181 tagaaaataa gatgagccaa atagcagaag ctaatgcata tctgcgggtt gatctttatg
     3241 atcatgcaga gttggagtta tctcatcata tcaagagttc tcttcagaat attttaaaag
     3301 tactaagttc tttttcacca ggtattaata gtcggaaagg aattagaaat taccatgaga
     3361 aatttatttc cagatatgga tatgaacagt tagtccctct tcaattactt ttgaattcta
     3421 ctagtggact tggatttcca aaagaatata gtcagacaaa agattttaaa caaaataatg
     3481 gagatagtcg gaatcaaaag ataacagaat ttttgcagag aaaatttgaa aaatctttaa
     3541 gagatggtaa agaaatcatc ctgaaggatg atgatttaaa agagctagat tttgatagag
     3601 aacagcagat atcaggggaa ttatactgtt tctatgattt taaaaataaa aagttggagg
     3661 ttagtggttt aggtgtttca ccgatgattg gagatacctt cggacgtttc catgctaaat
     3721 tgccaaatac tatagtcaca gaaaatgtaa ataaaacgaa agaaattttt gctaaagcct
     3781 atcctgatgc tattattact caactaacag aagtgccata ttttgggagg agtgctaatg
     3841 ttatggttag taatagtctt aaaagccatc agctggaatt gagaaactat gcttcaaaaa
     3901 aagatatgag tatcaatgat atttatgtag gtgcaaccag tgaggaatta tacttttatt
     3961 ctaaaaaata tggaaaaaga gttattttta taatgaatag tatgtttaat tatataaatg
     4021 gttccaaact tttacgcttc ttattagaag tttcaaattc tgactttcaa aatattaccc
     4081 cgattatgtt tggtaaccta gattcttata atcgtgtgcc cgctatcact tataaagata
     4141 ttattattaa accagaaaca tggaatatta gaaaatctga ggttaagact ttagactctc
     4201 tcaaaaattg gctatctgat aatgatgttc catctcttgt gcggatgaag tatgctgatc
     4261 aaattattta cttggatttg agtcggacta ttgatttaac tatgttattt cggagtatca
     4321 aaaaacacag ttctatacaa ttattagcta ctcattccaa atttgtaaac gatatgaagg
     4381 ttttggagct agttgtacct tttacaagga gtggtgtcaa tgctcgccag atttatcatt
     4441 atgctcagaa tatttatact ttggaggatt cagatagtaa agaaaaatat ttttacgtta
     4501 aaatttatgt taataaacaa cgacaagctt ctttcttaca aaaggaatat cctttattat
     4561 taaactattt gaaactccct gaaaatttgc aatggttcta tattagatat gaagatgacg
     4621 aaaaagacag catacgtttt agaatcagat atgtagaaga taaacaatta attcaacttt
     4681 attcacgctt tatagagtgg gcaacagaag cacggaaaaa tatacaaatt tcaggttatg
     4741 agattagtga atacatccct gaatcagcaa gatatggagg aaaaaaatat tcatcaatta
     4801 ttcattcttt cttccattac gatagtattt tgagtttact tttacaaaag aaaactgatc
     4861 aagccattga agtgagaact tctctcagta ttattcgcat gtttttaatg atgaaattaa
     4921 acttgcaaga tcaacaggaa ctcatgaaga acttgtttga tggagaacat aaacttaaat
     4981 atgaaaagga atatcataat tcaataagtt tattacttga tagtttgtgt ataagaaatc
     5041 agatagatga agctgatact ttctgtgtaa tgaatatgaa aataatcact gaagaagtta
     5101 gttcagttct taaacaagaa gacttaacag cagattggca gagaattcta ggaagtctta
     5161 ttcatatgag atgtaatcga gtatatggaa ttaatagtga gttagaaaga aaaacaatgt
     5221 ttattgttga caaagttatt aattcaaaaa gatatacaga tatgtttttg ggggtggata
     5281 atgatacaaa gtaagcgtgt agaaaaaatt aaaaatcttc taacagaaca aacttattta
     5341 tttgattatc aagaggtatt aaaaaaaaac agtcaagtaa aacaaacgga tttttggaat
     5401 ttactttcct tatcttcggg aataacttct ttattaatct tttatcaaga gtatgagaat
     5461 ttagaaaaga tagatttaaa gcagcaaaag cagtcattaa ttggactcat aagtcattat
     5521 gttaatcaaa tagcagataa atcctcttta tttgatggtt tagccggggt aggttttgct
     5581 attaattatg tctctaataa cggtaaatat tatcaagaac ttcttaatca aattgacagt
     5641 aaagttcgtc aaaacgttaa gcggaatctt gctgtctgta atagtgaaga gtatacaaat
     5701 cctacgaatt atgatgtaat ttctggaaat gctggagtag ctcgttatct gatggaaaga
     5761 gaatcttctg aaaattggca aatagttaaa atgattttag aaacatttta taaagctttg
     5821 gaacaaggat ggcgagtgca accaaaatat caatttttag aagctgaaaa gcagtattat
     5881 tcaaagggaa atataaattt tggtttagct catggggtat taggacctac aacgattatg
     5941 tctctttatc agcaaaggta cccacaagat acaagaaatg ctgagaaact tcaggaaact
     6001 tatcagctaa taaaaagata tgcccaagta agagatgagg gactgcgatg gccaatacga
     6061 tatgatttac ttcgtaaaga ggcttctttt atattaagaa atggttggtg ctatggagat
     6121 aatggcgttt ataatacact tttccttata ggaaaagtac tctcaaatca ggaaatttgt
     6181 gaaactgcta ggaaagtcat accatctatt ataagagatg attatgaaaa aatggaaagt
     6241 ccgacttttt gtcatggatt tgcgggaaaa gcaaatttct ttcttctgca atatcaaaga
     6301 actaacgaat caatattttt agttaaagca gaagaagaaa ttgataaaat actagtcatg
     6361 tataactctg aaaatatgtt tggatttaaa gatatagaag acaatattga taatactgga
     6421 gagaggttaa cttattggga taattttggt cttcttagtg gaactgttgg tattttattg
     6481 gttttgatgg aatattgtaa tattgtaaat gctggaaaag ttgcagaatg gaacaaaatt
     6541 tttctgttga cttaattaac tgaatggagg aataattatg gaagaacaaa atacagagaa
     6601 aaaaattctc ttatgcttaa cggggtctgg agccttgcta gggatagctg aatatattac
     6661 gtttttgact gtgcgtttta agcatgttcg agttattgtt tctgacaatg ctgcaaagat
     6721 gcttcccgtt gctgctatta cacaattgtg tgagaaagtg tacactgatg aagtttcctt
     6781 tatagataag caaaagaatc acatagcttt aactcgctgg gcagatataa cagttgtctt
     6841 accagctaca gcaaatatca ttggaaaagt tgctaatggt attgcagata actttatgac
     6901 aacaacgctt ctttcttcta gcaagccggt tttaatttat ccttgtatga ataatattat
     6961 gtgggaaaat ccagtagttc aaaaaaatgt cgaaacttta actggaacac aatataaagt
     7021 aattgttggg caagaatcag aatcttttga attagccagt ggaaaaatga aaaagaatat
     7081 tgcaattcca agtttggagg aattgcaaca agttatttta gaaaatttac aagaagagag
     7141 gtaagagtat gaagaagaaa ggattactag taataatctt tttaactttc tttttctttt
     7201 atcctaaagt taaagcggct gaatatacaa ttatatcaaa taacagtgaa caaactgtta
     7261 atgacttgaa taatctggga gttacagtta ataaccatat tgcggaaatt ggatatattg
     7321 aagcccaagg agatgttaac attgatcaga ttaaaaagtt gtcaaatatt caaagtatcc
     7381 agaatatggc tgatacatca cagaatatca cgactagagt tccttcaaca tatattaacc
     7441 agacgataca attgcctcag cttttttctt atcagtggga tatgcaaaaa attactaata
     7501 atggtgtttc atattcatta aataagggaa atcggaaaaa tgtaacagtt gctttagttg
     7561 attctggaat tgatgtagag cacaatgctt ttactgggat gattgatagt cgttcaaaaa
     7621 attttgtgcc tgctggaggc tatgagaata gcgaaaacag tgagactggg aatataagta
     7681 atattgatga taaaaaagga cacgggacgg ctattgctgg gcaaattgct gcaaatggtc
     7741 aaatccttgg tgtgtcacca ggaacgaacc ttcttgtcta tagagttttt ggaaaatcaa
     7801 aatcaaagga atgctggatt ttaaaagcaa ttattgatgc aacaaataat ggtgctaatg
     7861 ttattaatct aagtttaggg caatatatta agattcctaa tggtaatatc tgggagtctg
     7921 ctgaagcatt aggatataag tttgccattg attatgctac aagacataat gccatcgtcg
     7981 tggcagccac aggtaatgat ggattgagtg aagacaatgg agaaataaaa acttattata
     8041 ataatcaaca ttcaggacaa gatacatctc aaaattacac ggttgaagat tacccttctg
     8101 ttttacctaa tactattgca gttggttctt ctgataataa taatcaaaga tcatctttta
     8161 gtaattatta taatcaatct catagtaatt ttattttggc tcctggtggt ggaacaacct
     8221 tgttagacaa gtatggtcga gaagagtggt ataatcagaa actttttatg aaagagcaag
     8281 tcttatcaac aagtaataat ggaaattatg attatgtaga tggtacttct gtttcaacag
     8341 gaaaagtttc tggagaactt gcagaaatta ttagtaacta tcatcttcaa agaaactctc
     8401 caaaagctag aaggatctta ctaaatcaag ttaattatac taaagatggg tataaagaaa
     8461 taagcgttta caaagcttta cgaggtcact aaatgaaatg gttagaagtt ttgcagatta
     8521 gtaaaaaaga acgaattcta tatcttattg gttgtatatt ttcaattcta acaggtttga
     8581 ttactctcca gattacttac ttactgaaaa atttagttga taacaaatca tcttttaata
     8641 atttgttctt gttccttgtt ttgggattag ttctttttat catagatacc ggttcacagt
     8701 atctgatttc attgattggt aatcaagtgg tgtttaacag tcgaaataat atttggagaa
     8761 aaatctctga ttggacagat agtaaagatg attcttctga aatggcaggg caccttatta
     8821 atgatagtga acttatagaa aatttcataa tatctactat tcctcaaaca gtaaattcag
     8881 ttattgttgg atcaggatct ttagttatgc tatttattat taatagtaaa atgtccatag
     8941 aagtcatagg aatttgttta cttttattat tcattatgca accgttttct agaatattag
     9001 gtaaaataag taagaaaatt caggaggata aagctgaact tattaatgtt acctcacaac
     9061 tgagaggaca ggttaaaaca ataaaaagct ataatgcaca ggattatgcc cttcaaaaat
     9121 ttgatgagaa aaaccgcaaa ttatttcaag atatcttaaa tagaataaaa gtttttagcg
     9181 tttactctcc ttttttaaat atcttagttc tctttatgat tataactgtt gtttggttag
     9241 ggaatgcaga agtgcgttca gggaatctta ctgtgggttc agcgactatt tttgtcgttt
     9301 atatgacaca attaattaac ccgattatgc agttatcaca attagttgct catataggga
     9361 tgcttaatgg cggcgtagag cgtcttttag tgtataatca agctgttcca gaaaaaagtg
     9421 gaattaagaa aattgacgaa ataattgata tcgtatttga taatatctca tttgcttatg
     9481 atgatcaaaa aagtattatt gaaaatgtga atctaacttt tcaaaaaggt aattatattt
     9541 ccattgttgg tgaaagtgga gttggaaaat caactttact tgatctttta gagcataatt
     9601 atacaccatt aaaaggacaa atcttaataa acggaataaa cttagaagag ttggatatca
     9661 agacttttcg aaataaaata agctatgtat ctcaagaacc agcaattctt tctgggacaa
     9721 ttcgagaatt attagacttt aatcagcaac aacatacaga agctaatctt tggaatgttc
     9781 ttgatattgt agaattgaca gaacttatta gaaatttacc acaaaaatta gattctaaga
     9841 ttgatgaata tgggggaaat ctttccggtg gtcaaatgca gcgtatttca cttgccagag
     9901 gcctgttgaa aaaaggagat gttttattat tggatgaatc atttgctaat attgatgaag
     9961 aaacttgtct cagaataaaa tcaaaaattg ctgcttatgc tcaatcgcat aagcaaattg
    10021 ttattgaagt cattcataac ctaaatagaa taactcctaa tagtatcgtt tatcaattgg
    10081 ttgataaaaa actagaaatt ttgaggagag gattttaa
//
