LOCUS       BGC0000531              9746 bp    DNA     linear   BCT 14-JUL-2016
DEFINITION  Streptococcus mutans mutacin I biosynthesis gene cluster, partial
            sequence.
ACCESSION   BGC0000531
VERSION     BGC0000531
KEYWORDS    .
SOURCE      Streptococcus mutans
  ORGANISM  Streptococcus mutans
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 13:47:56
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 9746
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..9746
                     /db_xref="taxon:1309"
                     /mol_type="genomic DNA"
                     /organism="Streptococcus mutans"
                     /strain="UA140"
     protocluster    1..9746
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[3071:7308]"
                     /cutoff="20000"
                     /detection_rule="((LANC_like and (Lant_dehydr_N or
                     Lant_dehydr_C)) and not (YcaO or TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      3072..7308
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="((LANC_like and (Lant_dehydr_N or
                     Lant_dehydr_C)) and not (YcaO or TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
     cand_cluster    1..9746
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((LANC_like and (Lant_dehydr_N or
                     Lant_dehydr_C)) and not (YcaO or TIGR03604))"
                     /kind="single"
                     /product="lanthipeptide-class-i"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..9746
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000531"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..9746
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lanthipeptide-class-i"
                     /region_number="1"
                     /rules="((LANC_like and (Lant_dehydr_N or Lant_dehydr_C))
                     and not (YcaO or TIGR03604))"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            1016..1885
                     /gene="mutR"
     CDS             1016..1885
                     /codon_start=1
                     /gene="mutR"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR01716"
                     /gene_kind="biosynthetic-additional"
                     /note="positive regulator"
                     /product="MutR"
                     /protein_id="AAG48563.1"
                     /sec_met_domain="TIGR01716 (E-value: 1.6e-67, bitscore:
                     216.4, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKVNQSMELGELYRELRIARGLKIKDIACKNLSKSQLSRFENGQT
                     MLAADKLLLAISGIHMSFSEFGYALSHYEESDFFKRGNKLSELYVQKDIKGLKKLLEFN
                     DNHEVFDVYNRLNKLVIQVTIHLLDTDYIISDDDKNFLTTYLYNIEEWTEYELYIFGNT
                     MSILSSDDLIFLGKAFVERDKLYISLPSHKKNAELTFLNLILILLERKKLYQAIYFVEN
                     LEKLLNYQDMFAITFLKFLKKIITYFHDKSVDMSELEHYINIVEEINPTIASILKSNLN
                     QLLSSFSH"
     gene            2457..2654
                     /gene="mutA"
     CDS             2457..2654
                     /codon_start=1
                     /gene="mutA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gallidermin"
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /note="prepromutacin I"
                     /product="MutA precursor"
                     /protein_id="AAG48564.1"
                     /sec_met_domain="Gallidermin (E-value: 4.1e-26, bitscore:
                     80.3, seeds: 3, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSNTQLLEVLGTETFDVQEDLFAFDTTDTTIVASNDDPDTRFSSL
                     SLCSLGCTGVKNPSFNSYCC"
     CDS_motif       2457..2579
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence:
                     MSNTQLLEVLGTETFDVQEDLFAFDTTDTTIVASNDDPDTR"
                     /locus_tag="mutA_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       2580..2654
                     /prepeptide="core"
                     /locus_tag="mutA_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class I"
                     /score="18.60"
                     /molecular_weight="2345.7"
                     /monoisotopic_mass="2344.1"
                     /core_sequence="FSSLSLCSLGCTGVKNPSFNSYCC"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MSNTQLLEVLGTETFDVQEDLFAFDTTDTTIVASNDDPDTR
                     "
                     /leader_location="[2456:2579](+)"
                     /alternative_weights="2363.8"
                     /alternative_weights="2381.8"
                     /alternative_weights="2399.8"
     gene            2723..2917
                     /gene="mutA'"
     CDS             2723..2917
                     /codon_start=1
                     /gene="mutA_"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR03731"
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /product="MutA'"
                     /protein_id="AAG48565.1"
                     /sec_met_domain="TIGR03731 (E-value: 1e-19, bitscore: 59.5,
                     seeds: 23, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLNTQLLEVLGTKTFDVQEDLFEFNITDTIVLQASDSPDTHSKVG
                     SFSICPPRKTSVSFNSYCC"
     CDS_motif       2723..2845
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence:
                     MLNTQLLEVLGTKTFDVQEDLFEFNITDTIVLQASDSPDTH"
                     /locus_tag="mutA__lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       2846..2917
                     /prepeptide="core"
                     /locus_tag="mutA__lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class I"
                     /score="0.70"
                     /molecular_weight="2325.7"
                     /monoisotopic_mass="2324.2"
                     /core_sequence="SKVGSFSICPPRKTSVSFNSYCC"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MLNTQLLEVLGTKTFDVQEDLFEFNITDTIVLQASDSPDTH
                     "
                     /leader_location="[2722:2845](+)"
                     /alternative_weights="2343.7"
                     /alternative_weights="2361.8"
                     /alternative_weights="2379.8"
                     /alternative_weights="2397.8"
     gene            3072..6044
                     /gene="mutB"
     CDS             3072..6044
                     /codon_start=1
                     /gene="mutB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_N"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lanthipeptide_LanB_RRE"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1155:Lantibiotic dehydratase domain protein (Score:
                     671.4; E-value: 3.2e-203)"
                     /gene_kind="biosynthetic"
                     /note="modification enzyme for mutacin I"
                     /product="MutB"
                     /protein_id="AAG48566.1"
                     /sec_met_domain="Lant_dehydr_N (E-value: 8.6e-122,
                     bitscore: 396.3, seeds: 73, tool: rule-based-clusters)"
                     /sec_met_domain="Lanthipeptide_LanB_RRE (E-value: 6.3e-13,
                     bitscore: 38.5, seeds: 20, tool: rule-based-clusters)"
                     /sec_met_domain="Lant_dehydr_C (E-value: 5.2e-20, bitscore:
                     61.2, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNDFQFQDYFMYRKPLGNFSNFLSITDMMDPIELLHNDSIFAEGV
                     YLASPSLRSSINKLENQIASTKEKKNAKETIFQYYARYNTRPTPFGLFSSIGIGGFSTH
                     PRKEKSCYEKSVNVDLFWAYKVADKLESMPEILNTLKVVANNALQKSNDFWLLDTRSHF
                     GLMNSRSDIREDITVKSNQLIDYVINCTEEPISYQTLIDDIAEKFSQSSDDVKEYLQKL
                     IKEEFLITELKFSLIEDNPLDWFINILERDQNNSELLEKLTEIKAMIQDYTDRNIGEGN
                     NSILALENKMSQIVKANAYLRVDLYDHAELKLAQHTKSSLQNILKVLSSFSSAVNSQKE
                     IKNYHEKFIARYGYERLVPLQLLLNSTSGLGFPKGYSQTEVSKQNNEDSKNQKIIEFLQ
                     RKFEKALRDGKEIILSDDDLKDLNFDTEQQISGELYCFYNFKSKKLEVSSLGVSQILGN
                     TFGRFHSKLPNTIVTKNVNKTKEIFTEAYPNTIITQLNEVPYFGRGGNIMISNSLKSHQ
                     LELRNYTTKKEMSINDIYVGATSEELYFYSKKYEKRVIFVMNNMFNYINGSKLLRFLLE
                     VSNSDFQNITPITLGSLDSYNHVPAIIYKDIIIKPETWDIRKSEAKTLDSLKNWLTNNN
                     VPPFVRMKYTDQIIYLDLSRTIDLTMLFQSIKKHSFIQLLDVHSVCTNDTEILELVVPF
                     TRSDVNAHQIYHYAQNIYTLEDSGSKEKYFYAKIYVNKQRQTSFLQKEYPLLLNYLKLP
                     ENLQWFYIRYKDDGKDSIRLRIRYVEDKQLVQLYSRFIEWATKARKNIQISGYEISEYI
                     PESARYGGKKYSSIIHSFFYYDSILDLLLQKKAEQTIEVRTSLSIIRMFLMMKLSLQDQ
                     QKLIKNLFDGKHKLKYEKEYHNSISLLLDNLCTKNQTDEADIFCVMNMKKITEKISSVL
                     KQKDLTTDWQRILGSLIHMRCNRVYGINSELERKTMFIVDKVINSKRYTDMFLEVGNET
                     K"
     gene            6031..7308
                     /gene="mutC"
     CDS             6031..7308
                     /codon_start=1
                     /gene="mutC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: LANC_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1140:Lanthionine synthetase C family protein (Score:
                     242.9; E-value: 9e-74)"
                     /gene_kind="biosynthetic"
                     /note="modification enzyme for mutacin I"
                     /product="MutC"
                     /protein_id="AAG48567.1"
                     /sec_met_domain="LANC_like (E-value: 4.7e-53, bitscore:
                     169.2, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRQSKRVEKIKDIPTEQTYLFDYQEILKKVSQAKQTDFWNLLSLS
                     SGITSLLIFYQEYENLEGVNLKQQKQSLIGLISHYINQIAEKSSLFDGLAGVGFAINYI
                     SNNGKYYQKLLEQIDNRLRQNIERDLVNYKNEEYANPNELMMYVSGNAGVARYLMERES
                     SEDWRIVEMILEVFYKALEQGWRVQPKYQFLESEKQYYLKGNINFGLAHGLLGPTTIMS
                     LYQQRYPQNTRNAEKLQETYHFIKRYAQLRDEGLRWAIRYDLFRKEGSFILRNGWCYGE
                     NGIYNTLFLMGKVLSNQEICETAQKVIPSIIKDDYEKMGSPTFCHGFAGKANFFLLQYQ
                     RTKESIFLVKAEEEIDKILIVYNSENMFGFKDIEDNIDNTGERLTYWDNFGLLSGTVGV
                     LLVLMEYCNIVNAGKIAEWNKIFLLT"
     gene            7331..7897
                     /gene="mutD"
     CDS             7331..7897
                     /codon_start=1
                     /gene="mutD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Flavoprotein"
                     /gene_kind="biosynthetic-additional"
                     /note="flavoprotein, modification enzyme for mutacin I"
                     /product="MutD"
                     /protein_id="AAG48568.1"
                     /sec_met_domain="Flavoprotein (E-value: 3.7e-34, bitscore:
                     106.5, seeds: 143, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEEQNIEKKILLCLTGSGPLLGIAEYITFLTVRFKHVRVIISDNA
                     AKMLPVAAITQLCEKVYTDEVSFTDKQKSHIALTRWADITVVLPATANIIGKVANGIAD
                     NFMTTTLLSSSKPVLIYPCMNNIMWGNPVVQKNVEVLSGTQYKVIVGQESESFELASGK
                     MKKNIAIPSLDELRRVVLENLQEER"
     gene            7902..9245
                     /gene="mutP"
     CDS             7902..9245
                     /codon_start=1
                     /gene="mutP"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075:alkaline serine protease, subtilase family
                     (Score: 244.9; E-value: 3.2e-74)"
                     /gene_kind="biosynthetic-additional"
                     /note="protease for processing of premutacin I"
                     /product="MutP"
                     /protein_id="AAG48569.1"
                     /sec_met_domain="Peptidase_S8 (E-value: 1.6e-45, bitscore:
                     144.7, seeds: 43, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKKGLLVIIFLTFFFFYPKAKAAEYTIISNNSEQTVNDLNNLGV
                     TVNSHIAEIGYIEAQGDVNIDQIEKLSNIQSIQNMADTSQNITTRVPSTYINQTIQLPQ
                     LFSYQWGMQKITNNGVSYSLNKENRKNVTVALVDSGIDVDHNAFTGMIDSRSKNFVPAG
                     GYDNSESSETGNINDIDDKKGHGTAVAGQIAANGQIFGVSPGTNLLIYRVFGKSKSKEC
                     WILKAIIDATNNGANVINLSLGQYIKIPNGDIWESAEALGYKFAIDYATRHNVIVVAAT
                     GNDGLSDDNGEVKTYYNSQHSGQDMSQNDTVEDYPSVLPNAIAVGSSDNNNQRSSFSNY
                     YNQYQDNFILAPGGGTTLLDQYGQEEWYNQKLFMKEQVLSTSNNGNYDYADGTSISTGK
                     VSGELAEIISNYHLQGDSSKARSILLNQVNYTSDGYKEISTYKALRGY"
     gene            9246..>9746
                     /gene="mutT"
     CDS             9246..>9746
                     /codon_start=1
                     /gene="mutT"
                     /note="ABC transporter for secretion of mutacin I"
                     /product="MutT"
                     /protein_id="AAG48570.1"
                     /transl_table=11
                     /translation="MKWLEVLQISKKEKILYLIGCIFSIMTGLITLRITYVLKNLVDSK
                     SSFNNVFLFLVLGLVLFIIDAGSQYLISLIGNQVVFNSRNNIWKKISDWTDSKDDSSEM
                     AGHLINDSELIENFIISTIPQSLNSVIVESESLVMLFVINSKMSLEVIGICLLLLFIMQ
                     PFSR"
ORIGIN
        1 aaccaacaaa tttggaaggc tattcctgtg acaaccattg aaacagacct tgataccgcc
       61 aaggaaatgg gtgccatggc tctctttggt gaaaaatacg gtaaaaatgt gcgcgtggtt
      121 tctatcggcg actattctgt tgaactttgt ggtgggaccc atcttaaaaa tacttctgaa
      181 attggcatct tcaagattgt caaagaagaa ggtattggtt caagaactcg ccgtatccta
      241 actgtgacca gtaaggaagt ctttgaagtc tacgccaaga agaagacata cttaagaaat
      301 gccgcaacgc ttaaagcgcc tcaaatgaat caggtagcca acaaggtagc ttcgctgcaa
      361 gaccaattgc ataaattgca aaaggagaat gccgagctga aagaaaaagc agccgcagta
      421 gccgcaggcg atatcttcaa agatgttaaa gaagtgaatg gtcttcgcta tattgcgagc
      481 caagtagaag ttgccgatgc gggtgccctt cgcacttttg ctgatcaatg gaaacaaaaa
      541 gattactccg acgttcttgt tttagttgcc agcattggta aaaaggtaat gttcttgttg
      601 ccagcaagtc taaggatatc catgctggta atctcatcaa gactttggca ccaatcgttt
      661 caggtcgtgg tggtggtaaa ccagacatgg ccatggcagg tggttcagat gcctcagcca
      721 tcaaggattt gattgccgca gtagcaggga atttgtaaaa tttatcttgt cagaaaatca
      781 ttcaatgact tatcagtaca ctcctcagta taaaaactgg ggagtttttt aataatgccc
      841 aaatgtcact taatatttgg atgttttttt gtgatgaaca aatccttatc atttccttaa
      901 atttgttttt tatactaaaa gcgggaatga ttcaaaacta aaaaaagata aacgaagaat
      961 tgaaaaagtg atataatagc acagaagagg gcctttataa tgaaaggaga ctattttgaa
     1021 agtaaatcaa tcaatggaat taggtgaact ttatcgagaa ttaagaattg ctagaggttt
     1081 gaagataaaa gatatagctt gtaaaaatct gtccaagtca caactctcta gatttgaaaa
     1141 tggacaaacc atgttggcag ctgataaatt gctattagct atttcgggaa ttcatatgag
     1201 tttttcggaa tttggatatg ctttgagcca ttatgaggag agtgattttt tcaaaagggg
     1261 taataagtta tcagaattat atgtccagaa agatatcaaa ggattaaaaa agttattaga
     1321 atttaatgac aatcatgagg tatttgatgt ctacaatcgt ttaaataaat tggttattca
     1381 agttactatt catttgctag atactgatta cataatatca gatgatgata agaatttttt
     1441 aacaacttat ctatataata ttgaagagtg gactgagtat gaactttata tctttgggaa
     1501 tactatgtct atattgtcat ctgatgattt aatttttttg ggaaaagctt ttgtagaacg
     1561 tgataagttg tatatatctc ttcctagtca taagaaaaat gcagagttaa cttttttaaa
     1621 tttaatctta attttgcttg aaagaaaaaa attatatcaa gcaatctatt ttgtagagaa
     1681 tttagagaaa ttattaaatt accaagatat gtttgcaata acatttttaa aatttttaaa
     1741 aaaaattatt acttactttc atgataagtc agtagatatg tctgaattag aacattatat
     1801 taatatagtt gaagaaataa atcctacgat tgcttcaatt cttaaatcta atttgaatca
     1861 gcttttatca agttttagcc attaaagcca tcttgataaa ttttatatct ttcatattca
     1921 ttaaatgtgg agataatgaa aaagcaacgg ttatgctatc gctgcttttt ttgtgattag
     1981 aagctatgtt atcatggagt tatagtaatg aaacatagtg acagttcatc ctttcttatt
     2041 ataaaagtgg taataagaga agtggtaaac aaagagtagt aaaataatac gtttaaccat
     2101 aatatttcct cctttaaatt attataagat tcaaaaaggt aatattctta tatttgcaaa
     2161 tatgggataa aataatttta aaaaagcaga tttgcaattt taaaaaaata gaggctaatg
     2221 gtggtattat attattgtaa atatatgttt actcagtaat agtgatttac tattacaaca
     2281 gattttgttg ttatcttaga tatttctgct agcattagtt atctgtagat gtactactta
     2341 ataagtatat aattataatt atataataac tattatcaga ttaccgttaa aagttttctg
     2401 atatgcttct actgaacaat ttacgttcag ttacacacat gaaaaaggag gatattatgt
     2461 caaacacaca attattagaa gtccttggta ctgaaacttt tgatgttcaa gaagatctct
     2521 ttgcttttga tacaacagat actactattg tggcaagcaa cgacgatcca gatactcgtt
     2581 tctcaagttt gagtttatgt tcattaggat gtacaggggt gaaaaatcct agtttcaata
     2641 gttactgttg ctaagttgta caaaagattt agattgtgtc gcatgtcagc ggcacaatct
     2701 tttgatatta gagatattaa atatgttaaa cacacaatta ttagaagtcc ttggtactaa
     2761 aacttttgat gttcaagaag atttatttga gtttaatata acagatacta ttgtactgca
     2821 ggctagtgat agtccagata ctcatagtaa agtgggtagt ttcagtatct gtcctcctcg
     2881 aaagacctcc gtcagtttca atagttactg ttgttaacta taaattatac ttaaattgat
     2941 aggaaacttg gtcatgacat tatcatatgt tgatattgga agagaatcaa atttataaag
     3001 acaattaaat ctaaatttga tgaatattta gatgaattat tactaggttg acagtcatgt
     3061 taggagaaga gatgaacgat tttcaatttc aagattattt tatgtacaga aaaccattag
     3121 gcaacttttc taattttctt agtataactg atatgatgga tcctattgaa ttattacata
     3181 atgattcgat atttgctgaa ggggtatatt tggcttcccc atctcttaga tcatctataa
     3241 ataaattaga gaatcagatt gcaagtacta aggaaaaaaa gaatgcaaaa gagactattt
     3301 ttcaatacta tgcccgttat aacacgagac caactccgtt tggcttgttt tcgtccatcg
     3361 gaataggtgg tttttcgacc caccctagga aagagaaatc ttgttatgaa aaatctgtta
     3421 atgttgatct tttttgggct tataaagtag cagataaact agaaagtatg cctgaaattt
     3481 taaatacttt aaaagtagtt gctaataatg ctttgcaaaa gtcaaatgat ttttggcttt
     3541 tagatacacg aagtcatttt ggacttatga attcacgttc agatattcgt gaggacatta
     3601 cagttaagtc taatcagctt atagattatg ttattaattg cacagaagaa ccaattagct
     3661 atcaaacatt aattgatgat attgccgaga aattctctca atctagtgat gatgtaaaag
     3721 aatatttgca aaaattaatt aaagaggagt ttttaataac tgaattgaaa tttagtttga
     3781 ttgaagacaa ccctttggat tggtttatta atattttaga aagagatcaa aataactcag
     3841 aattacttga aaagttgact gaaataaagg caatgattca agattatact gaccgtaaca
     3901 taggtgaagg taacaattcg attttagctc tagaaaataa gatgagccaa atagtaaaag
     3961 ccaacgcata cctgcgagtt gatctttatg atcatgcaga gctgaagtta gcgcaacata
     4021 ccaagagttc tcttcagaat attttgaaag tactaagttc tttttcgtca gctgttaata
     4081 gtcaaaaaga aattaaaaat tatcatgaga aatttattgc caggtatgga tacgagcggt
     4141 tagtacctct tcaattactt ttgaattcta ctagtggact tggttttcca aaagggtata
     4201 gtcaaacaga agtttctaaa caaaataatg aagatagtaa aaatcaaaaa ataatagaat
     4261 ttttacagag aaaatttgaa aaagctttaa gagatggtaa agaaattatt ttgagtgatg
     4321 atgatttaaa agatttaaat tttgacacgg aacagcaaat atcaggagaa ttatattgtt
     4381 tctacaattt taaaagtaaa aagctagagg ttagtagttt aggtgtctca cagatacttg
     4441 gaaatacttt tggacgtttc cattctaaat tgccgaatac gatagtcaca aaaaatgtaa
     4501 ataagacgaa agaaattttt actgaggctt atccaaatac tattattact caattaaatg
     4561 aagtgccata ttttgggaga ggtggcaata ttatgattag taatagcctt aaaagtcacc
     4621 agttggaatt gaggaactat actactaaaa aagagatgag tatcaatgat atttatgtag
     4681 gtgcaaccag tgaggagtta tatttttatt ctaagaaata tgagaaaaga gttatttttg
     4741 tgatgaataa tatgtttaat tatataaatg gttctaaact cttacgtttt ttactagaag
     4801 tttcaaattc tgattttcaa aatattaccc cgattacgct tggtagtctg gattcttata
     4861 atcatgtgcc cgctatcatt tataaagata ttattattaa accggaaaca tgggacatta
     4921 gaaaatctga agctaagact ttagattctc tcaaaaattg gctaactaat aataatgttc
     4981 cgccttttgt acggatgaaa tatactgatc aaattattta tttagatttg agtcggacta
     5041 ttgatttaac tatgctattt cagagtatca aaaaacatag cttcatacaa ttattagatg
     5101 ttcattcagt atgtacaaac gatacggaga ttttagaatt agttgttcct tttacaagaa
     5161 gtgatgttaa cgctcaccag atttatcatt atgctcagaa tatttatact ttggaggatt
     5221 caggtagtaa agaaaaatat ttttacgcta aaatttatgt gaataaacaa cgacagacct
     5281 ctttcctaca aaaagagtat cctttattat taaactattt gaaactccca gaaaacttac
     5341 aatggttcta tattagatat aaagatgatg gaaaagacag catacgtctc agaatcagat
     5401 atgtagaaga taaacaatta gttcaacttt attcacgctt tatagagtgg gcaacaaaag
     5461 cacggaaaaa tatccaaatt tcaggttatg aaattagtga atatatccct gaatcagcaa
     5521 gatatggagg gaaaaaatat tcttcaatta ttcattcttt tttctattat gatagtattt
     5581 tggatttgct tttacagaag aaagcagaac aaactattga agtaagaaca tctctcagta
     5641 ttattcgtat gtttttaatg atgaaattaa gcttacaaga ccagcagaaa ctcataaaga
     5701 atttatttga tggaaaacat aaacttaaat atgaaaaaga atatcataat tcaataagtt
     5761 tattacttga caatttatgt acaaaaaatc agacagatga agctgatatt ttctgtgtaa
     5821 tgaatatgaa aaaaatcact gaaaaaatta gctcagttct taaacaaaag gacttaacaa
     5881 cagattggca gagaattcta ggaagtttaa ttcatatgcg atgtaatcga gtatatggaa
     5941 ttaacagtga gttagaaaga aaaacaatgt ttattgttga caaagttatt aattcaaaaa
     6001 gatatacgga tatgtttttg gaggtgggta atgagacaaa gtaaacgtgt cgaaaaaatt
     6061 aaagatattc caactgagca aacttattta ttcgattatc aagaaatatt aaaaaaagtc
     6121 agtcaagcaa aacaaacaga tttttggaat ttactttcct tatcttcggg aataacttct
     6181 ttattaatat tttatcaaga gtatgagaat ttagaaggag taaacttaaa gcagcaaaag
     6241 cagtcattaa ttgggcttat aagtcattat attaatcaaa tagcagagaa atcctcttta
     6301 tttgatggtt tagctggggt aggttttgct attaattata tctctaataa cggtaaatat
     6361 tatcaaaaac ttcttgaaca gattgacaac agactccgtc agaatattga acgggacctt
     6421 gtcaactata agaatgagga atatgcaaat cctaatgaat taatgatgta cgtttctgga
     6481 aatgctggag tagctcgcta cttgatggaa agagaatcct ctgaagattg gcgaattgtt
     6541 gaaatgattt tagaagtatt ttataaagct ttggaacaag gctggcgagt gcaaccaaaa
     6601 tatcaatttt tagaatctga aaagcaatat tatttaaagg gaaatataaa ctttggttta
     6661 gctcatggat tattaggtcc tacaacaatt atgtctcttt atcagcagag gtacccacag
     6721 aacacaagaa atgctgagaa acttcaggag acttatcatt ttataaaaag atatgctcaa
     6781 ctaagagatg aagggctacg atgggcaata cgatatgatt tgtttcgtaa agagggttct
     6841 tttatattac gaaatggttg gtgttatggc gagaatggca tttataatac actttttctt
     6901 atgggaaaag tactctcaaa tcaggagatt tgtgaaactg ctcagaaagt tataccatcc
     6961 atcataaaag atgattatga gaaaatggga agtccaacat tttgtcatgg gtttgctgga
     7021 aaagcaaatt tctttcttct gcaatatcaa agaactaaag aatcaatatt tttagttaaa
     7081 gcagaagaag aaattgataa aatattaatt gtgtacaatt ctgaaaatat gtttggattt
     7141 aaagatatag aagataatat tgataatact ggagagagat taacttattg ggataatttt
     7201 ggtcttctta gtggaactgt tggtgttcta ttagttttga tggaatattg taatattgta
     7261 aatgccggaa aaattgcaga gtggaataaa atttttcttt tgacttaatt aactgaacgg
     7321 agaaataatt atggaagaac aaaatataga gaaaaaaatt ctcttgtgcc taacaggttc
     7381 tggacccttg ttagggatag ctgaatatat tacgtttttg actgtgcgct ttaagcatgt
     7441 tcgagttatt atctctgata atgctgcgaa gatgcttcct gttgctgcta ttacacaatt
     7501 gtgtgagaaa gtgtatactg atgaagtttc ctttacagat aagcaaaaga gtcacatagc
     7561 tttaactcgc tgggcagaca taacagttgt cttacctgct acagcaaata taattggaaa
     7621 agttgctaat ggtattgcag ataactttat gacaacaact cttctttctt ctagcaagcc
     7681 agttttaatt tatccttgca tgaataatat tatgtgggga aatccagtag ttcaaaaaaa
     7741 tgttgaagtt ttatctggaa cccaatataa ggtaattgtt ggacaagaat cagaatcttt
     7801 tgaattagcc agtggaaaga tgaaaaagaa tattgcaatt ccaagtttgg atgaattgcg
     7861 acgggttgtt ttagaaaatt tgcaagaaga gaggtaagag tatgaagaag aaaggattac
     7921 tagtaataat ctttctaact ttctttttct tttatcctaa agctaaagct gctgaatata
     7981 caattatatc aaataatagt gaacaaactg ttaatgactt gaataattta ggagttacag
     8041 tcaatagcca tattgcggaa attggatata ttgaagctca aggagatgtt aacattgatc
     8101 agattgaaaa gctgtcaaat attcaaagta tccagaatat ggctgataca tcacagaata
     8161 tcacgactag agttccttca acatatatta accagacaat acaattgcct cagctttttt
     8221 cttatcagtg gggtatgcaa aaaattacta ataatggtgt ttcatattca ttaaataaag
     8281 aaaatcgaaa aaatgtaaca gttgctttag ttgattctgg gattgatgta gaccataatg
     8341 cttttacagg aatgattgat agtcgttcaa aaaattttgt gcctgctgga ggatatgata
     8401 atagtgaaag cagtgaaact ggaaatatta atgatattga tgataaaaaa ggccatggaa
     8461 cagcagttgc tgggcaaatt gctgcaaatg gtcaaatctt tggtgtgtcc ccaggaacga
     8521 accttcttat ctatagagtt tttggaaaat caaaatcaaa ggagtgctgg attttaaaag
     8581 caattattga tgcaacaaat aacggtgcta atgttattaa tctaagtttg gggcaatata
     8641 ttaagattcc taatggtgat atttgggagt ctgccgaagc attaggatat aagtttgcca
     8701 ttgattatgc cacaagacat aatgtcattg ttgtagcagc cacaggtaat gatggattaa
     8761 gtgatgacaa cggagaggtt aaaacttatt ataatagtca gcattcagga caagatatgt
     8821 ctcaaaatga cacggttgaa gattatcctt ctgttttacc taatgctatt gcagttggct
     8881 cttctgataa taataatcaa agatcatctt ttagtaatta ctataatcaa tatcaggaca
     8941 attttatttt ggctcctggt ggtggaacaa ctttactaga ccaatatggt caagaagagt
     9001 ggtataatca gaaacttttt atgaaagaac aagtcttatc aacaagtaat aatggaaatt
     9061 atgattatgc agatggtact tctatttcaa caggaaaagt ttctggagag cttgcagaaa
     9121 ttattagtaa ctaccatctt caaggagatt cttcaaaagc tagaagtatt ctactaaatc
     9181 aagttaatta tactagtgat ggttataaag aaataagcac ttacaaagct ttgcgaggtt
     9241 actaaatgaa gtggttagaa gttttgcaaa ttagtaaaaa agaaaaaatt ctttatctta
     9301 ttggttgtat attttcaatt atgacaggct taattactct acgaatcacc tacgtactta
     9361 agaatttagt tgacagcaaa tcgtctttta ataatgtgtt cttgtttctt gttttgggat
     9421 tagttctttt tatcatagat gctggttcac agtatctaat ttcattgatt ggtaatcaag
     9481 tcgtgtttaa cagtcgaaat aatatttgga aaaaaatttc tgattggaca gatagtaaag
     9541 atgattcttc tgaaatggca ggccacctta ttaatgatag tgaactgata gaaaatttta
     9601 taatttctac tattcctcaa tcattaaatt cagttattgt tgaatcagaa tccttagtta
     9661 tgctatttgt tattaatagt aaaatgtctt tggaagttat agggatttgc ttgcttttat
     9721 tgttcattat gcaacccttt tctaga
//
