LOCUS       BGC0000534              6359 bp    DNA     linear   BCT 14-JUL-2016
DEFINITION  Streptococcus mutans lantibiotic mutacin K8 gene locus, partial
            sequence.
ACCESSION   BGC0000534
VERSION     BGC0000534
KEYWORDS    .
SOURCE      Streptococcus mutans
  ORGANISM  Streptococcus mutans
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 13:48:13
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..6359
                     /db_xref="taxon:1309"
                     /mol_type="genomic DNA"
                     /organism="Streptococcus mutans"
     protocluster    1..6359
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[1242:4029]"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1243..4029
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
     cand_cluster    1..6359
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(LANC_like and DUF4135)"
                     /kind="single"
                     /product="lanthipeptide-class-ii"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..6359
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000534"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..6359
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lanthipeptide-class-ii"
                     /region_number="1"
                     /rules="cds(LANC_like and DUF4135)"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            338..496
                     /gene="mukA1"
     CDS             338..496
                     /codon_start=1
                     /gene="mukA1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) L_biotic_A"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) leader_d"
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /note="predicted lantibiotic precursor peptide"
                     /product="MukA1"
                     /protein_id="ABK59354.1"
                     /sec_met_domain="L_biotic_A (E-value: 1.5e-30, bitscore:
                     93.9, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="leader_d (E-value: 6e-08, bitscore: 21.7,
                     seeds: 16, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNTTNEMLELIQEVSLDELDQVIGGMGKGAVGTISHECRYNSWA
                     FLATCCS"
     CDS_motif       338..415
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence: MKNTTNEMLELIQEVSLDELDQVIGG"
                     /locus_tag="mukA1_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       416..496
                     /prepeptide="core"
                     /locus_tag="mukA1_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="4.60"
                     /molecular_weight="2703.1"
                     /monoisotopic_mass="2701.2"
                     /core_sequence="MGKGAVGTISHECRYNSWAFLATCCS"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MKNTTNEMLELIQEVSLDELDQVIGG"
                     /leader_location="[337:415](+)"
                     /alternative_weights="2721.1"
                     /alternative_weights="2739.1"
     misc_feature    338..>6359
                     /note="lantibiotic mutacin K8 gene locus"
     gene            523..675
                     /gene="mukA2"
     CDS             523..675
                     /codon_start=1
                     /gene="mukA2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) L_biotic_A"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) leader_d"
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /note="putative lantibiotic precursor peptide"
                     /product="MukA2"
                     /protein_id="ABK59355.1"
                     /sec_met_domain="L_biotic_A (E-value: 3.9e-24, bitscore:
                     73.3, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="leader_d (E-value: 5.6e-08, bitscore:
                     21.8, seeds: 16, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKQSDEMLELIQEVSLDELDQVIGGAGNGVIRTITQGCRMPNNMQ
                     VLFTC"
     CDS_motif       523..597
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence: MKQSDEMLELIQEVSLDELDQVIGG"
                     /locus_tag="mukA2_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       598..675
                     /prepeptide="core"
                     /locus_tag="mukA2_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="4.60"
                     /molecular_weight="2671.1"
                     /monoisotopic_mass="2669.3"
                     /core_sequence="AGNGVIRTITQGCRMPNNMQVLFTC"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MKQSDEMLELIQEVSLDELDQVIGG"
                     /leader_location="[522:597](+)"
                     /alternative_weights="2689.2"
     gene            715..870
                     /gene="mukA3"
     CDS             715..870
                     /codon_start=1
                     /gene="mukA3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) L_biotic_A"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) leader_d"
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /note="putative lantibiotic precursor peptide"
                     /product="MukA3"
                     /protein_id="ABK59356.1"
                     /sec_met_domain="L_biotic_A (E-value: 5.6e-30, bitscore:
                     92.0, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="leader_d (E-value: 5.8e-08, bitscore:
                     21.8, seeds: 16, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKQSNEMLELIQEVSLDELDQVIGGMGKGAVGTISHECRYNSWAF
                     LATCCS"
     CDS_motif       715..789
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence: MKQSNEMLELIQEVSLDELDQVIGG"
                     /locus_tag="mukA3_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       790..870
                     /prepeptide="core"
                     /locus_tag="mukA3_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="4.60"
                     /molecular_weight="2703.1"
                     /monoisotopic_mass="2701.2"
                     /core_sequence="MGKGAVGTISHECRYNSWAFLATCCS"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MKQSNEMLELIQEVSLDELDQVIGG"
                     /leader_location="[714:789](+)"
                     /alternative_weights="2721.1"
                     /alternative_weights="2739.1"
     gene            1064..1225
                     /gene="mukA'"
     CDS             1064..1225
                     /codon_start=1
                     /gene="mukA_"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) L_biotic_A"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) mature_d"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) lacticin_mat"
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /note="putative lantibiotic-like precursor peptide"
                     /product="MukA'"
                     /protein_id="ABK59357.1"
                     /sec_met_domain="L_biotic_A (E-value: 1.5e-26, bitscore:
                     81.1, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="mature_d (E-value: 6e-12, bitscore: 34.0,
                     seeds: 10, tool: rule-based-clusters)"
                     /sec_met_domain="lacticin_mat (E-value: 3.4e-11, bitscore:
                     31.6, seeds: 18, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKGTQLYLEALEALQEIKVEELDTFIGGGGRASNTISSDCRWNS
                     LQAIFSCC"
     CDS_motif       1064..1150
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence: MKKGTQLYLEALEALQEIKVEELDTFIGG"
                     /locus_tag="mukA__lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       1151..1225
                     /prepeptide="core"
                     /locus_tag="mukA__lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="1.70"
                     /molecular_weight="2468.7"
                     /monoisotopic_mass="2467.1"
                     /core_sequence="GGRASNTISSDCRWNSLQAIFSCC"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MKKGTQLYLEALEALQEIKVEELDTFIGG"
                     /leader_location="[1063:1150](+)"
                     /alternative_weights="2486.7"
                     /alternative_weights="2504.8"
                     /alternative_weights="2522.8"
     gene            1243..4029
                     /gene="mukM"
     CDS             1243..4029
                     /codon_start=1
                     /gene="mukM"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: LANC_like"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: DUF4135"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1070:lanthionine synthetase C family protein (Score:
                     698.9; E-value: 1.9e-211)"
                     /gene_kind="biosynthetic"
                     /note="putative lantibiotic modification enzyme"
                     /product="MukM"
                     /protein_id="ABK59358.1"
                     /sec_met_domain="DUF4135 (E-value: 2.9e-79, bitscore:
                     255.7, seeds: 79, tool: rule-based-clusters)"
                     /sec_met_domain="LANC_like (E-value: 3.8e-55, bitscore:
                     175.9, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDQKELLYSQFDVFNKQVIERYLPEVLDESDDIIQDIEEQISDYY
                     RSTLIYLINEKRIDGSLIGSSPESRYNYFTNVLCQQGMILDEIEERFPTITQRVVISLK
                     KYLELSKYVKEAFTADFSELLAEGYLDGVASDISSDDVKIKITGDIHNGNGVCIVAYQG
                     KKVVFKKKSSQPNQLLQTLEREVSQYLDKEVYFISPFLDKGEYFWEKFVSSKPLLSEEE
                     AKEFYCRVGYLLACAYMLNISDLHFENLISSHINPILVDVETVFSTSTFDTIANNDATF
                     KIIESSRDSVLFTGLLPVSEADKVFGGDTSGVLGGIMIGEARIVINHNRDDIRIEKQKY
                     KTENQDHLPYFSDSEGVKTYLNAEDYVDFIKSGFSELSEFFMHRKEFLKTLYSEYGHLQ
                     TRLLFRNTRDYSLIRQLLTSPVYCEQSHVLFEKMEDKFSEVDSHELCQSEEKQLLNMDI
                     PYFYAEIASRDVRDDEGIVWQLTRTALSQVIKKLDNLSSAVINEQLDLIEFSIKTPNAL
                     YSTELQDAYRDFENNQQTQDQDVLISGINELTDVILENEKNSQEDGSTNWLTLKVTDYD
                     AFELVPMDDSVYDGLAGMAIALSEVYDLVDDIRQEKIRLCLQRIFTTLSNSYLELQNQS
                     YFVGKLGLFSALSRISPITGQELPDFVLDGDQDYLVDLDVSTADFLSSFTNEVVALRNS
                     DIKIGNLNQALDKLDELKIISEDFISWDKLESNNVSLAHGNLGVEVALLCLAGNLERPE
                     ALQLFRKAKRFDDRQRLENGWVDKRNSDTSANWCHGSTGVLVARLVQLRLDDRYKLLSP
                     SERTALEADLQHASKQIIDLGFDMTNFSLCHGTSGNLLALSYYQSYLPENDAQRLRAIL
                     DKEYRKLHAFGLTKGWMCSFNTKYNVYGLMTGLPGILYSTAKFLKGDDSLDVLIPNL"
     gene            4052..6124
                     /gene="mukT"
     CDS             4052..6124
                     /codon_start=1
                     /gene="mukT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 129.6; E-value:
                     2.5e-39)"
                     /gene_kind="transport"
                     /note="putative lantibiotic transporter"
                     /product="MukT"
                     /protein_id="ABK59359.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 2.6e-40, bitscore:
                     126.3, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIILQNNEEDCLLACYTMLLNDLGHKVPLYEVYDKDTLPADGLS
                     VSYLLSLNDRFGVTINAYRASFDELLKVYRQRKQRMILHWNQEHFVILEKITSKQVTIV
                     DPAIGRVKYSRDEFLSHYSETMILVKRGDYFQPQSYKQIFWKYFRKTLKLKPVSFFLVS
                     LLFIQTGVLLFSVALRQMLADDFKFWVSMSLLAIVLLFQLIGYFIKNIALDRYNTDFDD
                     NYSKALFQKLLKKPLLYFRNHLNGGVSEKINFKSTLRDNVTLKIIPSCVSFISAIVVFA
                     YLMTISIKLTLILMTMIAIYAVISILLYRKQNEHNQAYLQYLIDFNSELQTDLDDIDYI
                     KIMRREGLTFGSWMRSNQQVTTKYSQILKIENLSQIIGTFFNYISLSSIIVIAVYYKVY
                     IKVSLPDLLIYQTSISLLISSIEQVKGAVFEITRLGVYAEKQSDLLKDSKPITVPSSGS
                     ADYLIKAEHLNFSYGAKKVYSDVNLTVNKGEKIAIVGKSGSGKSTLLLLLAGMLRYDGS
                     LEYGVKNFEDYLSVVLQNMTPRKGSILENLEWSYDDLAPVYQAMKDTSADEIINQLPNK
                     IHSKLLKQGKNLSGGQIQKLLITKSLLKENAIIFWDEAFSNLDEQSKNKIYNNVLQNDR
                     YSERTMLIISHHLDIVNYVDYIIYIDAATGDVIKDSHNHLMQSNENYADFITSKI"
     gene            6239..>6359
                     /gene="mukF"
     CDS             6239..>6359
                     /codon_start=1
                     /gene="mukF"
                     /note="putative component of the mutacin k8 transporter
                     complex responsible for immunity to mutacin K8"
                     /product="MukF"
                     /protein_id="ABK59360.1"
                     /transl_table=11
                     /translation="MDNVIELQNLTKVYNGVTTVDLKKITCASGEIYGFLGQMC"
ORIGIN
        1 aaaaataatg atatttgggt ctttaattcc gattaattct gtagtattct taataagtct
       61 cataagttct tcctaatgat ggtttggtcg cttttcatta tagttcttat gggacttttt
      121 gtgtacactc aaaaagctct ataatctcta aagagtaatt gctcattata gagattatag
      181 agcctgagtt tttttgttgt actatatttt acattttatc ttatgaaaga aattaaaaat
      241 aattttaggt tttcttaata ttatctttaa gataagcaca tacaatggaa ttgtaacaaa
      301 ggaagataca acaactacaa gaaaggaata cataataatg aaaaatacta ctaacgaaat
      361 gctggagtta atccaagaag tcagtcttga tgaactcgat caagttattg gtggcatggg
      421 taaaggtgct gttggtacta tctctcatga atgccgttac aattcatggg cattcttagc
      481 cacttgttgt tcataattta aaatcgagag aaggtaactc ttatgaaaca atcagatgaa
      541 atgttggaat tgatccaaga agtcagtctt gatgaactcg accaagttat cggtggtgca
      601 ggtaatggtg tcattcgtac tattacacaa ggatgtcgca tgcctaataa tatgcaagtt
      661 ctttttacat gctgatcata aaattttgtc aaattataga aggaaggtag tctaatgaaa
      721 caatcaaacg aaatgctgga attaatccaa gaagtcagtc ttgatgaact cgatcaagtt
      781 atcggtggta tgggtaaagg tgccgttggt actatctctc atgaatgccg ttacaactca
      841 tgggcattct tagccacttg ttgctcataa tttaaaataa gtagcttgtc ttcgttgtta
      901 cataaaaaga ggggaacctc tttttttatt gtgcttttaa gattcggaat taactttaac
      961 tttagtttct cttaatcttt tctttaagtg accaatttac aatagatatg taaacaaaat
     1021 agtttatcag tgatactcta gatttattag tgaggtaaaa aacatgaaaa aaggaacaca
     1081 actgtatttg gaagcacttg aagcattgca agaaattaaa gttgaggaat tagatacctt
     1141 tatcggtggg ggcggtcgtg cttccaacac tatttcctcc gattgtcgtt ggaatagttt
     1201 acaggctatt tttagctgtt gttagttaca aaggagtaag tcatggatca aaaagaatta
     1261 ttgtatagtc agtttgacgt ttttaataaa caagtcattg aaagatatct tccagaagta
     1321 ttggacgaga gtgatgatat cattcaagac attgaagagc aaatctcgga ctactaccgt
     1381 tcaacattaa tctacttaat taatgaaaaa cgcattgacg ggagcctaat tggcagttct
     1441 ccagaatcac gttataacta ctttaccaat gttctatgtc agcaaggtat gattcttgac
     1501 gaaattgaag aacgattccc aacaatcacc cagcgtgtcg tcattagtct taagaaatat
     1561 ttagaactaa gcaaatacgt aaaagaggca tttacagcag atttctctga gttgctagct
     1621 gaaggatatc ttgacggtgt agcgtcagat attagtagtg atgatgtcaa aattaaaata
     1681 actggggata ttcataatgg caacggtgtt tgtattgttg cttatcaagg gaaaaaagtg
     1741 gtttttaaaa agaaatcatc tcagccaaat caattattgc aaactttgga gcgtgaagtt
     1801 agtcagtatt tggataaaga agtttatttt atttctccat ttttggataa gggagagtat
     1861 ttttgggaaa agtttgtatc cagtaaacca ttgttatccg aagaagaagc taaggagttt
     1921 tactgtagag tagggtatct attagcctgt gcttatatgc ttaatatttc agatttgcat
     1981 tttgaaaatt tgatatcttc ccatataaat cctatattgg ttgatgtgga gactgttttc
     2041 tcaactagta cctttgatac gattgcaaac aatgatgcta cctttaagat catagaaagc
     2101 agtagagatt ctgtactatt tacaggccta cttcccgtat ctgaagcaga caaagttttt
     2161 ggaggagaca ccagtggtgt tttaggtggc attatgattg gtgaagcaag gattgtaatc
     2221 aatcataatc gcgatgatat tcgtattgaa aagcaaaagt ataaaacgga aaatcaagac
     2281 cacctccctt actttagcga tagtgaaggc gtcaagactt atctcaatgc agaggactat
     2341 gtggacttta ttaaatctgg attttcagag ctcagtgagt tctttatgca tcgtaaagag
     2401 ttcttaaaaa ctctttactc agaatatggt catcttcaaa cacggctttt atttagaaat
     2461 acaagagatt atagtttaat tcgacagttg ctgacctctc ctgtttattg tgaacaatcc
     2521 cacgttttat tcgaaaaaat ggaagataag ttttctgaag tggatagtca tgaactgtgt
     2581 caatctgaag aaaagcaatt gttaaatatg gatattccat atttttatgc tgagattgcc
     2641 agtcgtgacg ttagggatga tgaaggcatt gtttggcagt tgacaaggac agctttaagc
     2701 caagtcatta aaaaattgga taatctgagt tctgctgtta taaacgaaca gctggatcta
     2761 atcgagtttt ctattaagac tcccaacgct ctatatagca cagaattgca agatgcctat
     2821 cgagattttg aaaacaatca gcaaactcaa gatcaagacg ttctgatatc tggtatcaat
     2881 gagttaaccg atgttatttt ggagaatgaa aagaattccc aagaagatgg cagtaccaat
     2941 tggttgacat taaaggttac cgattatgat gcctttgagt tagtacctat ggatgattct
     3001 gtctatgatg gtttggcagg aatggctata gctttaagtg aggtttatga cttggttgat
     3061 gacatcagac aggaaaaaat aaggctttgt ttacagcgta ttttcacaac attatcaaac
     3121 tcttatctgg agttgcaaaa tcaatcctat tttgtaggga aattaggcct tttttctgcc
     3181 ttaagcagaa ttagccccat tactggacag gagctgcctg actttgtctt ggatggagat
     3241 caggattatc ttgtagactt ggatgtttca acggcagatt ttctatccag tttcaccaat
     3301 gaagtggtag cattacgaaa tagtgatata aaaataggaa atctcaatca ggcacttgat
     3361 aagcttgatg agttgaaaat tatctctgag gatttcatca gctgggataa attggaatca
     3421 aacaatgtta gcttagcaca tggcaactta ggtgttgaag tagctctgct ctgtcttgca
     3481 ggcaatttag agcgtcctga agctttgcag ttgttccgta aagctaagag atttgatgat
     3541 cggcagcgat tagaaaatgg ctgggtggat aaaagaaatt cagataccag tgctaactgg
     3601 tgtcatggct caacaggtgt attagtagcc agattagtgc aactccgact ggatgatcgc
     3661 tataaactac tatcgccatc tgagcgaaca gctctagaag ccgatttgca gcatgcttca
     3721 aagcaaatca ttgaccttgg ttttgatatg acaaactttt ctctctgcca tggaaccagt
     3781 ggcaatttgc ttgctctgag ctattaccaa tcttacttac cagaaaatga tgctcaaaga
     3841 ttaagggcta tcttggacaa ggaatatcgc aaactgcatg cctttggttt aacaaaggga
     3901 tggatgtgca gctttaatac aaaatataat gtctatggtt tgatgactgg actgccaggg
     3961 attctttatt ccacagcaaa atttttaaag ggagacgatt cgcttgacgt tttaatccca
     4021 aatctttaag aggtagaaag gtttatatgg tatgaagatt attttgcaaa ataatgaaga
     4081 agactgtctt ttagcctgtt acacaatgtt gcttaatgat ttaggtcata aagttccatt
     4141 atatgaagtg tatgataaag atacattgcc ggcagacggt ctcagtgtct cctacctctt
     4201 atcgctaaat gacaggtttg gtgttactat caatgcttat cgtgcctctt ttgatgaatt
     4261 gttaaaagtc tatagacagc gaaaacaaag gatgattctg cattggaatc aagaacattt
     4321 tgttattctt gaaaaaatca cctcaaaaca agtgactatt gttgacccag cgattggacg
     4381 agtaaagtat agccgtgatg agtttttaag tcattattct gaaacgatga ttcttgtaaa
     4441 aaggggggat tatttccagc ctcaatcgta taaacagatt ttttggaagt attttagaaa
     4501 gactctaaag ctaaagcccg tttctttttt tctagtgtca ctattattca ttcagacagg
     4561 tgtcctttta ttctcagtag ccttgaggca aatgttggca gatgatttca aattttgggt
     4621 tagcatgtcc cttttggcaa tagtacttct ctttcaatta ataggttatt ttataaagaa
     4681 tatagcctta gaccgatata acacggactt tgatgacaat tacagcaaag ccttatttca
     4741 aaaattacta aaaaaaccgc ttctatattt tagaaatcat ttgaatggcg gtgtctctga
     4801 aaaaataaat tttaaatcaa cacttaggga taatgtgacc ttgaaaatta tcccgtcatg
     4861 tgtcagtttt atctcagcca ttgttgtctt tgcttattta atgacgatat caatcaaatt
     4921 aaccctaata ttaatgacga tgattgccat atatgctgtt atttccatcc tactataccg
     4981 taagcagaat gagcataatc aggcgtattt acaatattta attgatttta attctgagtt
     5041 gcagacagac ctagatgata tagactatat caaaattatg aggagagaag ggcttacttt
     5101 tggttcatgg atgcgtagta atcagcaggt gacaacgaag tattctcaga tactcaagat
     5161 agaaaatctt tcacaaatta tagggacctt ctttaactat atcagcttgt cttcgatcat
     5221 tgtcattgcg gtatattaca aagtctatat caaagtatcg cttccagact tgttgattta
     5281 tcaaacgagt atctccttgt tgatatcttc aattgagcaa gtcaaaggtg ctgtctttga
     5341 aatcacacga ttgggagtat atgctgagaa acaaagtgat ttactgaagg acagtaaacc
     5401 tattaccgtg ccaagttcag ggtccgcgga ttatttaatc aaagcagagc atttgaactt
     5461 ttcttatggt gctaagaaag tttatagcga tgttaattta acagttaata aaggtgaaaa
     5521 aattgctatt gtagggaaat ctgggagcgg aaaatctact ctcctcttac tgttagcagg
     5581 tatgctacgt tatgatggct ctcttgaata tggtgtcaaa aattttgagg attatttgag
     5641 tgtggtgctt caaaatatga cgcctagaaa aggaagcatt ttagagaact tagagtggag
     5701 ctatgatgat cttgctcctg tttatcaggc catgaaagat acatcagctg atgaaattat
     5761 taatcagctg ccaaataaaa ttcactcaaa attgttgaaa caagggaaaa atctatcagg
     5821 cggtcaaatc cagaagctgc ttatcacgaa atccttgttg aaagaaaatg ccattatttt
     5881 ttgggatgaa gctttcagta atctcgatga acaaagtaaa aataaaatct ataataatgt
     5941 gttgcaaaat gacaggtatt cagaaagaac aatgctgatt atcagccacc atttagacat
     6001 cgtcaactat gttgactaca ttatttatat agatgctgca acaggcgatg tgattaagga
     6061 tagtcataac catctgatgc aaagcaatga aaactatgcc gattttatca cgtctaaaat
     6121 ttaaggttgc tttcaggttt tcttaatttt atctttaagt cggctgggta taataacaac
     6181 agtatcaaaa aggagcagtc ctaacgaaat actgaacgat aaccaaagga gtgaaaacat
     6241 ggacaatgtt attgaactac agaatttaac aaaggtatac aatggtgtga cgacagtaga
     6301 ccttaaaaag attacttgtg catcagggga aatatatggc tttcttgggc aaatgtgct
//
