Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0000535.1:
Cluster 1RiPP115016

BGC0000535, nisin A biosynthetic gene cluster from Lactococcus lactis. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: nisin A
PubChem ID: 16130280
SMILES string: Copy to clipboard
Molecular formula: C143H230N42O37S7
Average molecular mass: 3354.09 Da
Molecular activity: Antibacterial, Signalling

Class-specific details

Biosynthetic class(es):
RiPP
RiPP subclass:
Lantipeptide (cyclic)
Peptides in this cluster:
ADJ56352.1
RiPP core peptide sequence(s): ITSISLCTPGCKTGALMGCNMKTATCHCSIHVSK
Leader peptide length: 23
Follower peptide length: -1
Peptidase(s) involved in precursor cleavage: adj56357.1
Crosslinks in final product:
3=7 AA (Thioether)
13=19 AA (Thioether)
23=26 AA (Thioether)
8=11 AA (Thioether)
25=28 AA (Thioether)

Gene cluster description

nisin A (BGC0000535). Gene Cluster 1. Biosynthetic class = RiPP. GenBank HM219853, positions 828-14152. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Detailed annotation

nisA leader / core peptide, putative Class I
MSTKDFNLDLVSVSKKDSGASPR - IDhbDhaIDhaLCDhbPGCKDhbGALMGCNMKDhbADhbCHCDhaIHVDhaK

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Proven expression in natural host, Sequence-based prediction
MIxS-compliance:Unknown
Contact for this cluster:Aljosa Trmcic (Cornell Univeristy)

Literature references

1. Trmcic A et al. (2011) Expression of nisin genes in cheese--a quantitative real-time polymerase chain reaction approach. J Dairy Sci 94(1):77-85. doi: 10.3168/jds.2010-3677.