LOCUS       BGC0000536             13530 bp    DNA     linear   BCT 04-DEC-2008
DEFINITION  Lactococcus lactis DNA, nisin Q gene cluster (nisQ,
            niqB,niqT,niqC,niqI,niqP,niqR,niqK,niqF,niqE,niqG), complete cds.
ACCESSION   BGC0000536
VERSION     BGC0000536
KEYWORDS    .
SOURCE      Lactococcus lactis
  ORGANISM  Lactococcus lactis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Lactococcus.
COMMENT     ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2024-10-17 13:48:04
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 13530
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..13530
                     /db_xref="taxon:1358"
                     /mol_type="genomic DNA"
                     /organism="Lactococcus lactis"
                     /strain="61-14"
     subregion       1..13530
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="ribosomal"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..13530
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            173..346
                     /gene="nisQ"
     CDS             173..346
                     /codon_start=1
                     /gene="nisQ"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR03731"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) mature_a"
                     /gene_kind="biosynthetic-additional"
                     /product="nisin Q precursor"
                     /protein_id="BAG71479.1"
                     /sec_met_domain="TIGR03731 (E-value: 1.4e-23, bitscore:
                     72.3, seeds: 23, tool: rule-based-clusters)"
                     /sec_met_domain="mature_a (E-value: 6.5e-08, bitscore:
                     21.5, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTKDFNLDLVSVSKTDSGASTRITSISLCTPGCKTGVLMGCNLK
                     TATCNCSVHVSK"
     gene            453..3434
                     /gene="niqB"
     CDS             453..3434
                     /codon_start=1
                     /gene="niqB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_N"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lanthipeptide_LanB_RRE"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1155:Lantibiotic dehydratase domain protein (Score:
                     864.1; E-value: 2e-261)"
                     /gene_kind="biosynthetic"
                     /product="lantibiotic dehydratase"
                     /protein_id="BAG71480.1"
                     /sec_met_domain="Lant_dehydr_N (E-value: 2.9e-94, bitscore:
                     305.8, seeds: 73, tool: rule-based-clusters)"
                     /sec_met_domain="Lanthipeptide_LanB_RRE (E-value: 4.2e-11,
                     bitscore: 33.1, seeds: 20, tool: rule-based-clusters)"
                     /sec_met_domain="Lant_dehydr_C (E-value: 1.8e-26, bitscore:
                     82.9, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIQNSFKAQPFLVRNTILSVKDKQSFTEYTQVIETANNNKVFLEQ
                     LLIANPKLYDTLQKYNAGLLKKKRVKKLFESVYKYYKRSYLRSTPFGLFSETSIGTFSE
                     NSKSELNGETTKGVILDTQWLIRLVHQMERDFSRKLSFVINNANYEFGDRIFQVYTINS
                     SDLEEVNIKSTNVYQIISKTCESGFQKYEEICESVTASYGDEYRELSEQYINSLIVNHY
                     LVSNLQKDILSDFSWETFLNKVEEIDDSKKYLIPLKRVQKLIQEYSELEIGEGLEKLKE
                     IYQEMSQILANDNYIQIDLFSDSEITFDTEQKQQLERLADFIGNTTKSVNRTYLDDYKD
                     KFIEKYGVDQEVQIVELFDSTFGIGAPYNYTHPQNDFYELEPNTAYFSEEEREKYLSKY
                     VEAIKNNEAINFEDLESHYQKIAAEKNSKLQGLELFLDLAKKDEKDIFVLGNIIGNNNL
                     GGASGRFSALSPELKNYHKSIVDSIEKENESKGITSCEIVFLPENIRHANVMHTSIERK
                     RVLPVFTSTSHDEVQLTNIYIGIDDNEEFYARDISTQEVLKFYVTSMYNKTLFSNELRF
                     LYEISLDDNFGNLPWELIYRDFDYVPRLVFGDIVVSPAKWKIREEDMKTEEITKEFLQS
                     KGVPKEFYIVNGDNKVYLSQENLLDMSMLEAQIKKSAKRNDFVELQEYIEDGDVVSKRQ
                     KGRVSDVVVPFVRKTTPDNDSKKFTREKRVSVEQREKLPFTDWLYIKLYISTNRQDEFL
                     LSYLPEIQEIIENLGGQLFFLRYTDPQPHIRLRIKCSNLFLAYNSILEILKKSRENRVM
                     STFDISTYDQEVERYGGFETLELSEQIFCADSKTIPTLLTVTKEANNDWSVDDVSILMD
                     YRYLKWFFQNDNQEILKFLHLVSPEKVEENVNEKIVHYHNLLETDNLDGRIFSDKNFED
                     LKRTVEALFYKMVSQNYDTQKVYSIIDSIIHVHNNRLIGINRDKEKVIYYGLQRLFTSE
                     EYMK"
     gene            3445..5247
                     /gene="niqT"
     CDS             3445..5247
                     /codon_start=1
                     /gene="niqT"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 172; E-value:
                     2.7e-52)"
                     /gene_kind="transport"
                     /product="ATP-binding cassette transporter"
                     /protein_id="BAG71481.1"
                     /transl_table=11
                     /translation="MDEVKEFTSKQFFNTLLTLPSTLKLIFQLEKRYALYLIVLNAIAA
                     FVPLASLFIYQDLINSVLGSGRHLINIIVIYFIVQVITTVLGQLENYFSGKFDMRLSYS
                     INMRLMKTTSSLELSDYEQADMYNIIEKVTQDSTYKPFQLFNAIIVVLSSFISLLSSLF
                     FIGTWNIGVAFLLLIVPILSLILFLRVGQLEFLIQWQRANSERETWYIVYLLTHDFSFK
                     EIKLNNISNYFIGKFGKLKKGFINQDLAIARKKTYFNILLDFILNLINILTIFAMILSV
                     RAGKLLIGNLVSLIQAISKINTYSQSMIQNIYIIYNTSLFMEQLFDFLKRENVVQKNVE
                     RIETHYHDIETVKVSNLSYAYPNSNNFALKNVDLSFEKGELTAIVGKNGSGKSTLVKII
                     SGLYQPTKGKVQYDNRGNNLLSEEFYQRNISVLFQDFVKYELTVRENIGLSDLSSPWKD
                     EDIIKVLDNLGLDFLKVNDRYALDTQLGNWFQEGRQLSGGQWQKIALARTFFKKAAIYI
                     LDEPSAALDPVAEKEIFDYFVKLSENNISIFISHSLNAARKANKIVVMKDGQVEDVGNH
                     DDLLKRCQYYQELYYSEQYEDNDE"
     gene            5240..6481
                     /gene="niqC"
     CDS             5240..6481
                     /codon_start=1
                     /gene="niqC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: LANC_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1140:Lanthionine synthetase C family protein (Score:
                     326.9; E-value: 3e-99)"
                     /gene_kind="biosynthetic"
                     /product="lantibiotic cyclase"
                     /protein_id="BAG71482.1"
                     /sec_met_domain="LANC_like (E-value: 1.3e-71, bitscore:
                     230.7, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKKIIEESVEKIITEWDEKTRKNKDKFDFGELTLSTGVPGVILM
                     LSEIKDNGKSRIYQKKIDDYIEYVVSKLSIHGLLTGSLYSGAAGIALSILHFREDNKKY
                     ENLLNSFNRYIEYFVKEKIERFNLESITPPDYDVIEGFSGILSYLLLVNDEQYDNLKRL
                     IVNFLSDLTKENKGLMSLYIKSENQMSKAESEMYPLGCLNMGLAHGLAGIGSILAYAHI
                     KGYGDKSSLSALEKIISVYEKYELKEQNQFLWKDGLVADELKEGKVIREASFIRDAWCY
                     GGPGISLLYLYGGLALKNDYFVSKAEKILESAMQRKLGVDSYMICHGYSGLIEICLLFK
                     RLLNTKIFDPYIEDFKANSEQILKEYGDENGSGFLEGLSGCVLAVSEFEYPTQSTYWRQ
                     ALLLFDDFLKGERK"
     gene            6478..7215
                     /gene="niqI"
     CDS             6478..7215
                     /codon_start=1
                     /function="self-protection"
                     /gene="niqI"
                     /product="immunity protein"
                     /protein_id="BAG71483.1"
                     /transl_table=11
                     /translation="MKKYLLLIVALLGMTSLAGCYQTSQKEVRFEKGSYKNFVYDNKSY
                     FVTDKQISQGSVNNSKAKFYKLLIVDAKSEKVLSSSNKNSVTLAFNNIYQTSDKSLCMG
                     VNDKYYQVLPESNEGTVKALSLQNFDQTGDNSDDNFSIDKNDARKIDYKGNIYSVSDKS
                     VPDDELGEFQDVLAEVRVFDSTTGKTIPKSDWGKIDNGESISKQNRTEWDYGEIHSIKG
                     KAMTETFAIEINDDFKLATKAGN"
     gene            7217..9304
                     /gene="niqP"
     CDS             7217..9304
                     /codon_start=1
                     /gene="niqP"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075:alkaline serine protease, subtilase family
                     (Score: 262.1; E-value: 2e-79)"
                     /gene_kind="biosynthetic-additional"
                     /product="leader peptidase"
                     /protein_id="BAG71484.1"
                     /sec_met_domain="Peptidase_S8 (E-value: 9.6e-48, bitscore:
                     152.4, seeds: 43, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKILSFLFMVVSLGLSTTVYGEAINSPRSSSNNANTELISKNSN
                     TGHFSTEESTTDSGKLKEQKPVVKSTTTTESVESRTDTTKNLLQTSDTQKESKASLQET
                     QASSETSKNGSVTNKRVVPTPKAELSNQSKAVTSTQPTQQSKSLNDGKTKVSFADSSQI
                     SKENLANSKDESGVTGNSASLSYHETREGVYPRNNTVDNPKNQKVKTQPLLTSDSLEKK
                     ASDYTASHDFWDYQWDMKYVTNDGESYVLYQPSKNISVGIIDSGIMEDHPDLSNSLGNH
                     LENFVPKGGFDNEEPDETGNSSAISDEMGHGTEVAGQITANGSILGVAPGVTVNIYRVF
                     GESLSKSEWVAKAIRKAADDGNKVINISAGQYLMISGSYDDGTNDYQEYLDYKAAIDYA
                     TKKGSIVVAALGNDSLNTQDNQEMINYLKEYRNIKVPGKVVDAPSVFEDVIAVGGIDGS
                     GNISDFSNVAPGAIYAPAGTTVDLKKYGQDEFVNQGYYSKDWIFTTTNTGWYQYVYGNS
                     FAAPKVSGALALVADKYGIKNPKQLKKFLLTNSPEVNGVKVLNVVDLLNEKNKASNSDT
                     NKNHGISSNNKSTGSSQVSELGKGKMGQDKDKEIQNNTKKSYHGSDIENNQNEISKEVI
                     SADYSINQKRNNKRNLRGAVSVRSQEILPTTGDERGDFLPVLGALGIVSSGLLKRKKSK
                     N"
     gene            9370..10056
                     /gene="niqR"
     CDS             9370..10056
                     /codon_start=1
                     /gene="niqR"
                     /gene_functions="regulatory (smcogs) SMCOG1008:response
                     regulator (Score: 177.4; E-value: 5.1e-54)"
                     /gene_kind="regulatory"
                     /product="response regulator"
                     /protein_id="BAG71485.1"
                     /transl_table=11
                     /translation="MYKILIVDDDQEILKLMKTALEMRNYEVVTRQEVSLPLNIDDFQG
                     FDLILLDIMMSNIEGTEICKRIRREVSIPIIFVSAKDTEEDIVNGLGIGGDDYITKPFS
                     LKQLVAKVEANIKREERNKQAVQDFSEVRRDLGTITFYLEERRVCVNGQTIPLTSREYD
                     ILELLSRRTSKVYTREDIYDEVYDEYADALFRSISEYIYQIRNKFAPYNIDPIKTVRGL
                     GYKWHD"
     gene            10049..11392
                     /gene="niqK"
     CDS             10049..11392
                     /codon_start=1
                     /gene="niqK"
                     /gene_functions="regulatory (smcogs) SMCOG1003:sensor
                     histidine kinase (Score: 93.9; E-value: 2.1e-28)"
                     /gene_kind="regulatory"
                     /product="histidine kinase"
                     /protein_id="BAG71486.1"
                     /transl_table=11
                     /translation="MTKKYSMRRRVWQSVIEIVIGTFLLIILLLGLTFFLRQIGQISGS
                     GTVRLSLDSDDLTVSDIKRDMAHYPYDYIIFDNDTSKILGGRYVKSDVSSFESSKQLSK
                     SVVKGEITYTYSSNKQFTLILRQSSIPEFTNHTLRSIYYNQFTYLFFFIGEIILISFSV
                     YRLIREFSKNFQSVQKIALNMGKITTFPEQEQSKITEFDRVLTNLYAKSEELALLIEAE
                     RREKKDLSFQVAALSHDVKTPLTVLKGNIELLEMTEMNEQQNDFIGSMKNSLAVFDKYF
                     NSMISYTKLLNDENDYKMKISLEDFLTDLSVELEELSATYQVDYQLNVKTELETFHGNT
                     LALSRALTNVFVNACQYAKDGEKKVSLSICDDEKYLYFVIWNNGQPFSDQAKKNAGKLF
                     FTEDAGRSGKHYGIGLSFAQGVALKHQGNLILSNPENGGAEVVIKIRK"
     gene            11490..12167
                     /gene="niqF"
     CDS             11490..12167
                     /codon_start=1
                     /function="self-protection"
                     /gene="niqF"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 177.9; E-value:
                     4.2e-54)"
                     /gene_kind="transport"
                     /product="immunity protein"
                     /protein_id="BAG71487.1"
                     /transl_table=11
                     /translation="MRVEIQNLSKEYKEKQVLQDISFNIESGTVCGLLGVNGAGKSTLM
                     KILFGLISADSGKICFDGQQKASNQIGALIEAPAIYMNLSAFDNLKTKALLFGISDKKI
                     HETLEVIGLAETGKKRAGKFSLGMKQRLGIGMAILTEPHFLILDEPTNGLDPDGIAELL
                     NLIAKLKAQGVTVLISSHQLHEVSKVADQIVILNQGRICYNHANNQEDDIEQLFFKIVH
                     GGM"
     gene            12169..12897
                     /gene="niqE"
     CDS             12169..12897
                     /codon_start=1
                     /function="self-protection"
                     /gene="niqE"
                     /product="immunity protein"
                     /protein_id="BAG71488.1"
                     /transl_table=11
                     /translation="MKRMLASETLKLKRSGTLRLAMIIPFVTLFIAFLMGGMQIFAAFS
                     IYWWEAGFLFLLISLLFLYDKKSEEKAGNFQNVNSEDLSWKIHLVKMLLIWLRGLIASL
                     VLAVLLYLVAWIFQGIVVVDFMKVSVALLAILLAAAWNLPLLYVLSKWFNTYVLIAANS
                     LLCLIVAPFIAQTPIWFLLPYTYHYKVVESLLHIKPAGDLLEKTVNFNIWEVLLPIGLS
                     IVVTIGLSYVLKGVLEHDKK"
     gene            12884..13528
                     /gene="niqG"
     CDS             12884..13528
                     /codon_start=1
                     /function="self-protection"
                     /gene="niqG"
                     /product="immunity protein"
                     /protein_id="BAG71489.1"
                     /transl_table=11
                     /translation="MIRSECLKLKNSSGLYLVFLFTLLEVLTIPIYLAFGRSIVSMTDL
                     SLMTLLFFPLLAAILSILTFEQESLANHFQEIKANKKTSRIWLSKLVVVDLLLFFPSAM
                     IWLITGISQGKGGEGLLIASASWLMAIFLNHFHLLLTFVLNRGGNIIVAIVEILLIVFA
                     SNKVLLGAYWCPIALPVNFVVTGGFNYLMAVVIWIVLCTLIIVSLPKNAIR"
ORIGIN
        1 aaatcttcac aatgcctaaa gcatttatag tctgtttttg aagaaaatct aaattggatt
       61 gtcgaggata gtttaggaaa aaattttttt gctctgaacn aattctgaaa ttcgttagat
      121 ataatggttt tgttcaaagg aactacaaaa tatattaaag gaggtactca aaatgagtac
      181 aaaagatttc aacttagatt tggtatctgt ttcaaaaaca gattctggcg cttcaacacg
      241 tattaccagc atttcgcttt gtacaccagg ttgtaaaaca ggtgttctga tgggatgtaa
      301 cctgaaaaca gcaacttgta attgtagcgt tcacgtaagc aaataaccaa atcaaaggat
      361 agtattttgt tgacttaggt aaagatacta tcctattttt ataagttatt tagggtttct
      421 aaatgactta caaaagtaaa gagaggaaaa acatgattca aaactcattt aaagcacagc
      481 cctttttagt aagaaataca attttatccg ttaaagataa gcagagtttt actgaataca
      541 cccaagtcat tgagactgcg aataataata aagtcttttt ggagcagtta cttatagcca
      601 atccaaaact ctatgatacc ttgcagaaat ataatgctgg tctgttaaag aaaaaaagag
      661 taaaaaaatt atttgagtct gtttataagt attataagag aagctattta cgttctactc
      721 cattcgggtt gtttagtgaa acttcgattg gtaccttttc agaaaattca aagtcagagt
      781 taaatggaga aactacaaag ggggtgatat tggacactca gtggttaatt cgtttggttc
      841 atcaaatgga acgtgacttc tcaagaaagc tatcatttgt tattaataac gcgaattatg
      901 aatttgggga ccgaattttt caagtttata ctattaacag tagtgatctt gaagaggtaa
      961 atattaaatc cacaaatgtt tatcaaataa tatccaagac ttgtgaaagt ggttttcaaa
     1021 aatatgaaga aatctgtgaa agtgtaacgg cgagctacgg agatgaatat agggagcttt
     1081 cagaacaata tattaatagt ctgatagtca atcattattt ggtttctaat ctgcaaaaag
     1141 atatattatc agatttttcc tgggaaactt ttctgaataa agttgaggag atagatgata
     1201 gcaaaaagta ccttattcct ttaaaaagag tccaaaagct aattcaagag tactctgaat
     1261 tagaaattgg cgaaggtctt gagaagctga aagaaatata tcaagaaatg tcacaaattc
     1321 ttgcgaacga taattatatt caaattgatt tatttagtga tagtgaaata acttttgata
     1381 ctgagcaaaa gcagcagttg gagcgtttag ctgacttcat aggaaatacg acaaaatctg
     1441 taaacagaac atacttggat gactataaag ataagtttat tgaaaagtat ggagtagatc
     1501 aggaggtaca gatagtagaa ttatttgatt caacatttgg tataggagcc ccatataatt
     1561 acactcatcc tcaaaatgat ttttatgagt tagaaccaaa cacagcttac ttctcagaag
     1621 aggaaagaga aaagtacctc agtaagtatg tggaagccat taaaaataat gaggcaatta
     1681 attttgaaga tttagaatct cattaccaaa aaatagcagc ggaaaagaat agcaaacttc
     1741 aagggctaga attattttta gatcttgcaa agaaggacga aaaggatatt tttgtcttag
     1801 gaaacattat tggaaataac aaccttggag gggcatctgg taggttctca gcactatccc
     1861 cagagttaaa aaattatcat aaatccatag tagattctat tgagaaagaa aacgagagta
     1921 aaggaattac atcctgtgaa attgtgttct tacctgaaaa tattaggcat gctaatgtta
     1981 tgcatacgtc aattgagaga aaaagggtac ttccagtttt tacaagtacg agccatgatg
     2041 aggttcagtt aaccaacatc tatattggaa tagatgacaa tgaagaattt tacgcacgag
     2101 atatttcaac tcaagaggta ctaaagtttt acgttactag tatgtataat aaaacattat
     2161 ttagcaacga acttagattc ctctacgaaa tttcattgga tgataatttt ggtaacttgc
     2221 cttgggaact tatttacaga gattttgact atgttccccg tttagtattt ggagacatag
     2281 ttgtttctcc tgctaagtgg aaaattcggg aggaagatat gaaaactgaa gaaataacaa
     2341 aggaatttct tcaaagtaaa ggagttccta aggagtttta tatcgtcaat ggagataata
     2401 aagtttattt atcacaagaa aatctgttgg atatgagtat gttagaggca caaataaaga
     2461 agagtgctaa aagaaatgat tttgtagagc tccaagaata tattgaagat ggtgatgtgg
     2521 taagtaaaag gcaaaaaggt agggtttcgg atgtagttgt tccttttgtt agaaagacaa
     2581 caccagataa tgatagtaag aaatttacaa gagaaaaaag ggtttcagtt gaacagcgtg
     2641 agaaattgcc cttcacggat tggctttaca ttaagttgta tatttctaca aaccgtcaag
     2701 atgaattttt actatcctat cttccagaaa ttcaggaaat aatagaaaat ctgggaggac
     2761 aactattctt tctaagatat actgaccctc agcctcacat acgattgcgt ataaaatgct
     2821 caaacttgtt tttagcctat aattccattc ttgaaatttt aaaaaagagt agagaaaata
     2881 gggtaatgtc aacttttgat atttctacct atgaccaaga ggtagaaaga tatggtggct
     2941 ttgaaacatt agaattatcc gagcaaatat tttgtgctga ttctaaaact attccaactc
     3001 tacttacggt tacaaaagag gctaataatg attggagtgt tgatgatgta tcaatcttga
     3061 tggattatag gtatctgaaa tggtttttcc agaacgataa tcaagaaatc cttaaatttt
     3121 tgcatttagt tagtcctgaa aaagttgagg aaaatgtcaa tgaaaagatt gtacattatc
     3181 ataatcttct ggaaaccgat aatctggatg ggcgcatttt tagtgataag aactttgaag
     3241 atttgaagcg tactgtagaa gctctatttt acaaaatggt atctcaaaat tatgatactc
     3301 agaaggttta ttcaattatt gatagcatca ttcacgttca taacaacaga ttaattggca
     3361 ttaaccgaga taaagagaaa gtgatttatt atggccttca aaggttattt acttcggaag
     3421 aatacatgaa atgaggaatg ataaatggat gaagtgaaag agttcacatc aaaacaattt
     3481 tttaatacgt tgcttaccct tccgagtacc ttgaaattaa tttttcagtt agaaaaacgg
     3541 tatgctctct atctaattgt tctaaatgct attgctgctt ttgttccatt agcaagtctc
     3601 tttatctacc aagatttgat aaattctgtg ttagggtcag gaaggcatct tatcaatatt
     3661 attgtcatct atttcattgt ccaagtgata accacagttt tgggacaatt agaaaattat
     3721 ttcagtggga aatttgatat gcggctttct tacagcatta atatgcgact tatgaaaact
     3781 acctcatctc ttgaattaag cgattatgaa caggcggata tgtataatat cattgaaaag
     3841 gttacacaag atagcactta taaacctttt cagctattca atgctatcat tgtagtgctt
     3901 tcatcgttta tttcattatt atctagtttg ttttttattg ggacatggaa cattggagta
     3961 gcatttttac tccttattgt tccaatatta tctttgatcc tcttccttag agtgggacaa
     4021 ctagagtttt tgatccagtg gcaaagagct aactctgaaa gagagacatg gtacattgtc
     4081 tatttattga ctcatgattt ttcttttaaa gaaatcaagt tgaataatat tagcaactat
     4141 tttattggca aattcgggaa gctaaaaaaa ggttttatca accaagattt agcgattgcc
     4201 cgtaaaaaga catacttcaa tattttgctg gattttatat tgaacttgat aaatattctt
     4261 acaatatttg ccatgatcct atcagttaga gcaggaaaac ttctcattgg taatttggta
     4321 agcctcatac aggctatttc caaaattaat acctattctc agtcaatgat tcaaaatatt
     4381 tacatcatct ataataccag tttgtttatg gaacaacttt ttgactttct aaaaagagaa
     4441 aatgtggttc aaaagaatgt agagaggatt gaaacgcact atcatgatat agaaactgtt
     4501 aaagtgtcca atttgtccta tgcttatccc aattcaaata attttgcgtt aaaaaatgtt
     4561 gatttatctt ttgaaaaagg agagttaaca gctatagtag ggaaaaatgg ttcagggaaa
     4621 agtacattag tgaaaataat ttcaggatta taccaaccga caaagggcaa agtccaatat
     4681 gataacaggg gaaataattt gttgtctgag gagttttatc agagaaatat atcagtacta
     4741 ttccaagatt ttgtgaagta tgagttaacg gtgagagaga atatcggatt gagtgacttg
     4801 tcttctccat ggaaagatga ggatattatc aaagtgctag ataatttagg gcttgacttc
     4861 ctgaaagtca atgatcggta cgcccttgat acgcaactag gaaattggtt tcaagaaggt
     4921 cgtcaacttt caggagggca gtggcaaaaa attgcgcttg caaggacatt ctttaagaaa
     4981 gctgcaattt atattttgga tgaaccaagt gctgcccttg atcccgtggc tgaaaaagaa
     5041 atatttgact actttgtcaa gctatcagaa aataatattt caatttttat ttctcatagt
     5101 ttgaatgctg ctcgaaaagc aaataaaatc gtggttatga aagatggaca agttgaagat
     5161 gttggaaatc acgatgacct tctgaaaaga tgtcagtatt atcaagaact ttactattca
     5221 gagcaatatg aggataatga tgaataaaaa aattattgaa gaaagtgttg aaaaaatcat
     5281 cactgaatgg gatgagaaaa ctagaaaaaa taaagataaa ttcgattttg gagagttgac
     5341 cctttctaca ggggtgccag gtgtaatttt aatgttatct gagataaaag ataatgggaa
     5401 atctaggata taccagaaaa aaatagacga ttatattgaa tatgttgtga gtaaactctc
     5461 aattcatgga cttctaacgg gctcacttta ttcaggagcg gctggtattg cattgagtat
     5521 tttacatttt cgagaagaca acaaaaaata tgagaatctt cttaatagct tcaatagata
     5581 cattgaatat tttgtcaaag aaaaaattga aagatttaat ttggaaagta ttactcctcc
     5641 agattatgac gtgattgaag gcttttctgg aatactttcc tatcttcttt tagtcaacga
     5701 cgagcagtat gataatttaa aaagactcat cgtcaatttt ctgtcagatt tgactaaaga
     5761 aaataaaggc ctaatgtcac tttacataaa atcagagaat caaatgtcca aagcagaaag
     5821 tgagatgtat ccattagggt gtttgaacat ggggctggca catggtcttg cgggaatagg
     5881 tagcatttta gcttatgcgc atataaaagg gtacggtgac aaatcatcat tatctgcttt
     5941 agaaaagatt atttccgttt atgaaaaata tgaacttaaa gagcaaaatc agtttctttg
     6001 gaaagatggg cttgtagcag atgaattaaa agaagggaaa gtaattaggg aagcaagctt
     6061 cattagggat gcatggtgtt atggaggtcc aggtattagt cttttatact tatatggggg
     6121 attagcacta aaaaatgatt attttgtgtc taaagcagaa aaaatattgg aatcagccat
     6181 gcaaagaaaa cttggtgttg actcatatat gatttgtcat ggatattctg gtttaattga
     6241 aatttgcttg ctatttaagc ggttattaaa tacaaaaata tttgaccctt acatagaaga
     6301 ttttaaagct aatagtgaac aaattcttaa agaatatgga gatgagaatg ggtcgggttt
     6361 tcttgaagga ttaagtggtt gtgtcttagc tgtgtcggaa tttgaatatc caactcaatc
     6421 tacttattgg agacaagcac tgttactctt tgacgatttc ttgaaaggag agagaaaatg
     6481 aaaaaatatt tattgttaat tgtagctctg ctaggaatga caagcttagc agggtgctac
     6541 caaacaagtc aaaaagaagt gcgcttcgaa aaaggaagct ataaaaattt tgtttatgat
     6601 aataagtcat actttgtgac cgataagcaa atttctcaag gcagcgtaaa caattccaaa
     6661 gcaaaatttt acaagctgtt gattgttgac gcgaaaagcg agaaagtttt atccagcagc
     6721 aacaaaaata gtgtgacttt agcttttaac aatatttatc aaacttctga caagtcgctc
     6781 tgcatgggtg ttaatgataa atactatcaa gtgctgccag aaagtaatga gggaacggtt
     6841 aaagctttaa gcctgcaaaa ttttgaccag acaggcgata attccgatga caatttctcc
     6901 attgataaaa acgacgcacg aaaaattgac tataaaggca atatttacag tgtatcagac
     6961 aaatccgtgc ctgacgacga attgggagaa tttcaagatg ttctagctga agtccgtgtc
     7021 tttgattcga caacaggtaa aactattccc aagtctgact ggggaaaaat tgacaacggc
     7081 gaatcgattt ccaaacagaa tcggacagaa tgggactacg gcgaaattca ttcgattaag
     7141 ggaaaagcta tgactgaaac atttgccatt gagataaatg atgactttaa gctcgcaacg
     7201 aaggcaggaa attagagtga aaaaaatatt aagttttctt tttatggttg tttcgttggg
     7261 attatcaaca actgtgtatg gggaggcaat aaactcacca cggtcttcat cgaacaatgc
     7321 gaacacagaa ttaattagta agaattctaa tacaggtcac ttctcaacgg aggagtcaac
     7381 aactgattca ggcaagctaa aagagcagaa acccgtagta aaatcgacta ccacaaccga
     7441 atcggttgaa agtagaactg acactactaa aaatttgttg cagacttcag atactcaaaa
     7501 agaatcaaaa gcttcattgc aagaaactca agccagttct gaaacaagca aaaatggaag
     7561 tgttacaaat aaaagagtag ttccaacgcc taaagctgag ctgtctaatc aaagcaaagc
     7621 ggtcacttca acacaaccaa ctcaacaaag taaaagccta aatgacggga aaactaaggt
     7681 tagtttcgct gattcatcac agatttctaa agagaatcta gctaattcta aagatgaatc
     7741 tggtgtaaca ggcaattctg cgtcattatc atatcatgag actagggagg gagtatatcc
     7801 tagaaacaat acagtagata atcccaaaaa tcaaaaagta aaaacacagc ctctattgac
     7861 aagcgattct cttgaaaaga aagcgagtga ttatactgct tcacatgatt tttgggatta
     7921 ccaatgggat atgaaatatg tgacaaatga tggagaaagc tatgtgctct accagccttc
     7981 aaagaatatt tctgttggaa taattgattc tggaattatg gaggaccacc ctgatttgtc
     8041 aaacagtcta ggaaatcact tagaaaattt tgttcctaag ggagggtttg ataatgaaga
     8101 acctgacgaa actggaaatt ctagcgctat ttcggatgag atggggcatg ggactgaggt
     8161 tgcaggtcag attacagcaa atgggagtat tttaggagta gcaccgggtg ttactgtaaa
     8221 tatctataga gtatttggcg aaagtctttc gaaatcggaa tgggtagcta aggcaataag
     8281 aaaagctgca gatgacggaa acaaagttat caatataagt gctggacagt accttatgat
     8341 ctcaggctca tacgatgatg ggacaaatga ttatcaagaa tatctcgatt ataaagcggc
     8401 aatagattat gcgacaaaaa agggaagcat agttgttgcg gcccttggta atgatagttt
     8461 aaacacacaa gataaccaag aaatgataaa ctatcttaag gagtatagaa atataaaggt
     8521 tcctgggaaa gttgtggatg ctccaagtgt attcgaagat gtaatagcgg taggtggaat
     8581 agatggatct ggcaatattt ccgattttag taatgttgct ccaggtgcta tctatgcgcc
     8641 tgctggaaca acagttgatt tgaaaaaata tggtcaagat gaatttgtca atcaaggtta
     8701 ttattcgaaa gactggattt ttacaactac taatactggt tggtatcaat atgtatatgg
     8761 aaactcattt gcggctccca aggtatctgg agcactggca ttagtggctg ataaatatgg
     8821 tataaagaat cctaagcaac tgaaaaaatt ccttctcacg aattctccag aagttaatgg
     8881 ggtaaaagtg ttaaatgttg ttgatttgtt gaatgaaaaa aataaggcta gtaattcgga
     8941 tacgaataaa aatcatggta tttcttctaa caataaatca actggaagtt cacaagtatc
     9001 tgaacttgga aagggaaaaa tgggacaaga taaagataaa gaaattcaga ataatacaaa
     9061 gaaaagctat catggttctg atattgaaaa taatcagaat gaaatttcta aagaagtaat
     9121 ttcagctgat tatagcatta atcaaaaaag gaataataaa cgaaacttga gaggggctgt
     9181 ttccgtacga agtcaagaaa ttttacctac cactggggat gaaagaggag attttttacc
     9241 tgttttaggt gcattgggta ttgtaagctc tggtctattg aaaagaaaaa aatcaaaaaa
     9301 ttgactaact atatttcttc aagatgaatg gtataatgga gtaaaggcta aacaatcgga
     9361 ggtaaagcgg tgtataaaat tttaatcgtt gatgacgatc aagaaatttt aaagttaatg
     9421 aagacagcac tcgaaatgag aaactatgaa gttgtgacgc gccaagaagt ttctcttccc
     9481 ctgaatattg atgattttca ggggtttgat ttgatattgc ttgatatcat gatgtcaaat
     9541 attgaaggga cagaaatctg caagagaatt cgtcgggaag tatcaattcc aattattttt
     9601 gttagtgcaa aagatacaga agaagacatt gtaaatggct taggtattgg tggagatgac
     9661 tatattacca agccttttag tcttaaacag ctggttgcaa aagtagaagc aaatataaag
     9721 cgcgaggagc gcaataaaca agcggttcag gatttttcag aggtccgtag ggacctaggg
     9781 acaatcacat tttatttgga agaacgccgc gtatgcgtaa atggtcaaac tattccgttg
     9841 accagtcgtg aatatgacat ccttgagtta ctatcgagac gaacttcaaa agtttatacg
     9901 agagaggata tttatgatga agtgtacgat gagtatgcag atgctctttt tcggtcaatt
     9961 tcagaataca tttatcagat taggaataag tttgcaccat ataatattga tccgataaaa
    10021 acggttcgag gactagggta taaatggcat gactaaaaaa tattccatgc gtcggcgtgt
    10081 atggcaatct gtcattgaaa ttgtcattgg tacttttcta cttatcattc tactattagg
    10141 tttaactttc ttcttaagac agattggtca aatcagtggt tcaggtactg ttcgtttatc
    10201 tttagattca gatgatctca cggtctctga tattaaacgt gacatggcac actaccctta
    10261 tgactatatt atctttgaca atgatacgag taaaatttta ggaggacgct acgtcaaatc
    10321 agatgtatca agtttcgaat cttcaaagca actttcaaaa agtgttgtaa aaggggaaat
    10381 cacttatact tattcaagta ataagcagtt tacacttatt ttaagacaaa gcagtattcc
    10441 agagtttact aatcatacgc tgcgttcaat ttattacaac cagttcactt acctcttctt
    10501 tttcattgga gaaattatcc ttatcagttt ttcggtttat cgactcattc gagagttttc
    10561 aaaaaacttt caatctgttc aaaaaatcgc gttgaatatg gggaaaataa ccacttttcc
    10621 cgaacaagaa caatcaaaaa ttactgagtt tgatcgggtt ctcacgaatt tgtatgcaaa
    10681 aagtgaggag ttagctctac taattgaagc cgagcgtcgc gaaaaaaaag atttatcttt
    10741 ccaagttgca gcactttcgc atgatgtcaa gactccttta acagttttaa aaggaaatat
    10801 tgaactgctt gagatgactg aaatgaatga acagcagaat gatttcattg gctcaatgaa
    10861 aaacagcttg gctgtttttg ataaatattt taattcaatg attagttaca caaaacttct
    10921 caatgatgaa aatgattaca agatgaaaat ttctctagaa gactttttga cagatttgtc
    10981 ggttgaacta gaagaattat cagcaaccta tcaggtagat taccagttaa atgtaaagac
    11041 agaattagaa acttttcacg ggaatacatt agctctgagt cgggcactta ccaatgtttt
    11101 tgtcaatgcc tgtcaatatg ccaaagatgg tgaaaagaaa gtcagtttga gcatttgtga
    11161 tgacgaaaag tatctctatt tcgttatttg gaataatggt cagccctttt ctgaccaagc
    11221 gaaaaaaaat gcaggtaaac tattttttac ggaagatgct ggacgaagtg ggaaacatta
    11281 tggtattggt ctctcttttg cccaaggtgt tgccttgaaa catcaaggga atctaattct
    11341 cagtaatcca gagaatggtg gggcagaagt tgtcataaaa ataaggaagt aatctttagg
    11401 gattactttt tattgtattc tgaatgaatt ctgaaaatca ttttatatac tttacttaga
    11461 tataaaatga aataaagtga ggatataaaa tgcgagttga aattcaaaat ctctctaaag
    11521 agtataaaga aaagcaggta ctacaagaca tcagttttaa cattgagtcg ggaacagttt
    11581 gtggactatt aggggttaat ggtgcgggta aatcgacttt gatgaaaata ctatttggct
    11641 tgatttccgc agattctgga aaaatttgct ttgatgggca gcaaaaagca agcaatcaga
    11701 taggagcttt gattgaagct ccagcgattt atatgaattt atccgccttt gataatctta
    11761 aaacgaaagc tttgcttttt ggaatttcag ataagaaaat tcatgaaaca ctggaagtga
    11821 ttggtttggc agagacagga aagaaaagag ctggaaaatt ctctttaggg atgaaacaaa
    11881 ggttgggaat cggtatggcc attctgacag aacctcattt cttgattctt gatgagccta
    11941 ctaatggctt agatcctgac ggaattgcgg aattgttaaa cttaattgct aaacttaaag
    12001 cgcaaggtgt gacagtttta atttctagtc atcaattgca tgaggtgagc aaagtagctg
    12061 atcaaattgt cattttgaat caaggcagaa tttgttataa ccatgcgaat aatcaagaag
    12121 acgacattga acagctcttc tttaaaattg tacacggagg aatgtgacat gaaaagaatg
    12181 ttggcatcag agacattaaa attaaaaaga tctggaacgc ttagattagc aatgattatt
    12241 ccttttgtga cactatttat agcgtttctt atggggggaa tgcagatttt tgccgcattt
    12301 tcaatttatt ggtgggaagc tggcttttta ttccttttga taagtttact ttttctctat
    12361 gataaaaaat cagaggagaa agctgggaac tttcaaaatg tgaattcaga agacttgagt
    12421 tggaaaattc atttggtgaa aatgttgttg atctggttga gagggctgat tgcgagctta
    12481 gtcttagccg ttttactcta tttggtcgct tggattttcc aaggaattgt ggttgtggat
    12541 tttatgaaag tgagtgtggc actgcttgcc attttactag cagccgcttg gaatctccca
    12601 ttactatacg tgctttccaa gtggtttaac acctatgtac tgatagctgc gaattctttg
    12661 ctttgcttga ttgttgcacc tttcatcgca cagacaccta tatggttttt actgccatac
    12721 acttatcact ataaagttgt ggagagcttg ctacatatta aaccagcagg agatttgctg
    12781 gaaaagacgg taaatttcaa tatctgggaa gttttattac ctattggact ttccatagtg
    12841 gtgacaatag ggctttcata tgtgcttaaa ggagtattag aacatgataa gaagtgaatg
    12901 tctcaaatta aaaaatagct ctggacttta tttggttttc ctctttacct tattagaggt
    12961 attgacaatc ccaatatatt tagcttttgg acgaagcatt gtttcaatga ccgatttgtc
    13021 actcatgaca cttttgttct ttccattatt ggctgcaatt ttgtctattc ttacctttga
    13081 gcaggagagt ttagccaatc attttcaaga aataaaagca aataaaaaaa ccagcagaat
    13141 ttggctatca aagctggttg tagttgactt gcttttgttc tttccttcag caatgatctg
    13201 gctaattacg ggaatctcac agggaaaagg cggagaaggg ctgttaattg catcagctag
    13261 ttggttgatg gcgattttcc tcaatcattt tcatctttta ttgacctttg ttctcaatcg
    13321 aggaggcaat attattgttg cgattgtcga aatcttgctc attgtttttg ctagtaataa
    13381 agttttgcta ggagcttatt ggtgccctat tgccttacct gtcaattttg tggtaacagg
    13441 aggatttaac tatttaatgg ctgtcgttat ctggattgtt ttgtgtacat taattattgt
    13501 atcattaccc aaaaatgcga tcagataaaa
//
