LOCUS       BGC0000537             15653 bp    DNA     linear   BCT 06-FEB-2006
DEFINITION  Streptococcus uberis nisin U gene locus, complete sequence.
ACCESSION   BGC0000537
VERSION     BGC0000537
KEYWORDS    .
SOURCE      Streptococcus uberis
  ORGANISM  Streptococcus uberis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 13:48:31
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 15653
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..15653
                     /db_xref="taxon:1349"
                     /mol_type="genomic DNA"
                     /organism="Streptococcus uberis"
                     /strain="42"
     protocluster    1..15653
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[7380:13415]"
                     /cutoff="20000"
                     /detection_rule="((LANC_like and (Lant_dehydr_N or
                     Lant_dehydr_C)) and not (YcaO or TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      7381..13415
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="((LANC_like and (Lant_dehydr_N or
                     Lant_dehydr_C)) and not (YcaO or TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
     cand_cluster    1..15653
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((LANC_like and (Lant_dehydr_N or
                     Lant_dehydr_C)) and not (YcaO or TIGR03604))"
                     /kind="single"
                     /product="lanthipeptide-class-i"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..15653
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000537"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..15653
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lanthipeptide-class-i"
                     /region_number="1"
                     /rules="((LANC_like and (Lant_dehydr_N or Lant_dehydr_C))
                     and not (YcaO or TIGR03604))"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            594..1979
                     /gene="nsuP"
     CDS             594..1979
                     /codon_start=1
                     /gene="nsuP"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075:alkaline serine protease, subtilase family
                     (Score: 221; E-value: 5.9e-67)"
                     /gene_kind="biosynthetic-additional"
                     /note="nisin U leader peptidase"
                     /product="NsuP"
                     /protein_id="ABA00872.1"
                     /sec_met_domain="Peptidase_S8 (E-value: 3.4e-38, bitscore:
                     121.0, seeds: 43, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKRKCPLLKIFLTLVIAILFNISISSEVNASENKGTIYNVVIREN
                     KSIESVKNIFQEQGIKVIDKIPEINFLIIYTEKSEDTIKKENTDYIDDIVRDNTMTVKP
                     NITYLYGEKVYTNTNLDFWGNQWDMQKSISTGSKFKHKSSGKATIGVIDSGITYDNPDI
                     FSHIISVQNFTSNLETGVVDKQNVVDKTGHATSVVGQISSSGEYLGIAPEMKIRMYRVF
                     DEGNAQDQWILKALIQAAKDDVDVINLSLGEYLLRGSTDEDDNTALINIYQRAINYAHN
                     QGSVVVASVGDEGIDLQNQDKLKTYLGGLRGKDYSQVNGTVQDIPAELDNVVTVGSVDD
                     NDLVSSFSNKGKDSIDIYATGGGSRELSQIGYEKWVSDRLFEKEWILVPTLEGKYTYAY
                     GTSISTPKVSAALGLIIEKYNLKDQPDEAIRILYDNCFLSNNGNNGQIRLLNITNFCNN
                     SVK"
     misc_feature    594..14133
                     /note="nisin U gene locus"
     gene            2007..2705
                     /gene="nsuR"
     CDS             2007..2705
                     /codon_start=1
                     /gene="nsuR"
                     /gene_functions="regulatory (smcogs) SMCOG1008:response
                     regulator (Score: 187.6; E-value: 4e-57)"
                     /gene_kind="regulatory"
                     /note="response regulator"
                     /product="NsuR"
                     /protein_id="ABA00873.1"
                     /transl_table=11
                     /translation="MYKILVIDDDFEILKLMRTILEMKNYNVTTYQEVKLPIEMSDFKG
                     FDLILLDVMMPDIDGLQICKQIRESVSSPIIFVSAKDSEDDIVSGLNLGGDDYITKPFS
                     VNQLVAKVAAHLKREERHRNSKSNENVVRELLPITFYLQEKIVCINGEVISLTCREYDI
                     LDLLASKKNKVYTREEIYKRVYDEESDALFRSISEYVYQIRVKFSPYGINPIKTIRGIG
                     YKWNNEKVFN"
     gene            2686..4032
                     /gene="nsuK"
     CDS             2686..4032
                     /codon_start=1
                     /gene="nsuK"
                     /gene_functions="regulatory (smcogs) SMCOG1003:sensor
                     histidine kinase (Score: 127.3; E-value: 1.6e-38)"
                     /gene_kind="regulatory"
                     /note="histidine kinase"
                     /product="NsuK"
                     /protein_id="ABA00874.1"
                     /transl_table=11
                     /translation="MKKYSISKRILSTIIEIVIGTIANLFLLLVLSHILVLTGQLNTYG
                     TDYHISVKDKRSIDSIKNKIDHTVFDYVLFDSKTGKLVSGKYQKQDLQHYKEVFDTGSS
                     GSDGSILYTDYGDSNFTFVVRRPTIPEFVNPNFRHVSYNSVSYLLFMGLELLLIFSSVI
                     RLLKEFTKNFRLIEKISLNMGIPEMDIERKETEMREFNNILLTLYQKDEELTQLLEMEK
                     KDKEDLSFQVAALAHDIKTPLTVLKGNIELLEMTDLDEKQLDFMSSMNHSVDIFEKYFN
                     SMINYARLLIEDKDYQEDIDLSEFLDELSLECRDIMTSKKIDFQIVNESQVEVIKGNRL
                     NLDRALLNILSNALRYCSEKGEVVLSISESPNFVSFEIWNNGIPFTEEALKDGGKLFYT
                     ENKGRNDKHYGIGLSFAQKIAMRHQGQLVLSNPQKGGAQVTLLIKNFQR"
     gene            4124..4816
                     /gene="nsuF"
     CDS             4124..4816
                     /codon_start=1
                     /gene="nsuF"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 174.2; E-value:
                     5.7e-53)"
                     /gene_kind="transport"
                     /product="NsuF"
                     /protein_id="ABA00875.1"
                     /transl_table=11
                     /translation="MEVIIKNLRKSYKDKEVLKNVSFRIEEGTICSLLGINGAGKSTIM
                     KILFSLESADSGEVIFNGEYKTPEGTDRFDIGALIESPAIYLNLSAFDNLKTRALLHGI
                     SDERILEVLHLVGLDGTGKKKAAHFSLGMKQRLGLGMAIITNPDLLILDEPTNGLDPDG
                     IKELLNLIMTLKKSGMTILLSSHQLSEVSKVADKIVILNKGEIFYDDINKGTNDLENLF
                     FRIVHGGE"
     gene            4803..5564
                     /gene="nsuE"
     CDS             4803..5564
                     /codon_start=1
                     /gene="nsuE"
                     /product="NsuE"
                     /protein_id="ABA00876.1"
                     /transl_table=11
                     /translation="MGVNKMINIFKSEWLKQKSSSDKKLLIFIPFLAIFIAFILAGPIN
                     LESFSIYWWEGIFLYTLFGLLFLADFNAEENAGSFQNISFGRNTFTIYIAKILLKLKDI
                     LISTFIFTIIFYCISCLFSGMITVDIAKDFICLLLIGFSSAWTLPLLYFLSKWVNSYLL
                     LAVNSLICLLVAPLIAQTSIWFIFPYTYHYKIAHAFLHLKPSGDLDIAASGIGMMTILV
                     AMGLSILVFVTFLSLLKWRITNEQNYKKRIT"
     gene            5533..6225
                     /gene="nsuG"
     CDS             5533..6225
                     /codon_start=1
                     /gene="nsuG"
                     /product="NsuG"
                     /protein_id="ABA00877.1"
                     /transl_table=11
                     /translation="MNKIIKSELLKWKGSFALNLILVLAIIQLFTIPLYLHFTNNLVLI
                     ENIIFLPMLGYCILASILSILLYEQEEKANSFQNIKSEKNRSSIWGMKLISTDLLMVLL
                     GVPVWIVVGFGFDRLSYYAYVGVITWLLLVLLNHFHMLLSLIMTKVGNLVIAFIECLFI
                     IFATNKVFLKSFWLPIALPVNLILEFGGNKILMIFAYLVGFIILLYFCNLTVITEFENQ
                     KKKKLYKK"
     gene            6968..7135
                     /gene="nsuA"
     CDS             6968..7135
                     /codon_start=1
                     /gene="nsuA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR03731"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) mature_a"
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /note="nisin U lantibiotic"
                     /product="NsuA"
                     /protein_id="ABA00878.1"
                     /sec_met_domain="TIGR03731 (E-value: 2.3e-25, bitscore:
                     78.0, seeds: 23, tool: rule-based-clusters)"
                     /sec_met_domain="mature_a (E-value: 2.4e-08, bitscore:
                     22.8, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNEDFNLDLIKISKENNSGASPRITSKSLCTPGCKTGILMTCPL
                     KTATCGCHFG"
     CDS_motif       6968..7039
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence: MNNEDFNLDLIKISKENNSGASPR"
                     /locus_tag="nsuA_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       7040..7135
                     /prepeptide="core"
                     /locus_tag="nsuA_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class I"
                     /score="27.80"
                     /molecular_weight="3029.7"
                     /monoisotopic_mass="3027.5"
                     /core_sequence="ITSKSLCTPGCKTGILMTCPLKTATCGCHFG"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MNNEDFNLDLIKISKENNSGASPR"
                     /leader_location="[6967:7039](+)"
                     /alternative_weights="3047.7"
                     /alternative_weights="3065.7"
                     /alternative_weights="3083.7"
     gene            7381..10356
                     /gene="nsuB"
     CDS             7381..10356
                     /codon_start=1
                     /gene="nsuB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lanthipeptide_LanB_RRE"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1155:Lantibiotic dehydratase domain protein (Score:
                     796; E-value: 7.4e-241)"
                     /gene_kind="biosynthetic"
                     /note="lantibiotic biosynthesis protein"
                     /product="NsuB"
                     /protein_id="ABA00879.1"
                     /sec_met_domain="Lant_dehydr_N (E-value: 2.8e-91, bitscore:
                     296.0, seeds: 73, tool: rule-based-clusters)"
                     /sec_met_domain="Lanthipeptide_LanB_RRE (E-value: 1.2e-10,
                     bitscore: 31.7, seeds: 20, tool: rule-based-clusters)"
                     /sec_met_domain="Lant_dehydr_C (E-value: 8.9e-22, bitscore:
                     67.5, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNRYIANNFLVRNTITETHELLECDYNSFLETIRENKIFQEQLF
                     VASRSLYESLQKYYSGDSMKRKKINQLSESVYKYYKRSKERSTPFGLFSETSIGSFSST
                     EKLNLNGKTLKKVLLDSEWLIRLVFKIEKEYSRELAYKINSANYQFGDRVVQLFSINDT
                     KTEEVNIKFTKVYQLIYELCCDKYVYFNCIIEKLVESYGEEYRDIATSYIMSLIDSHFL
                     ISNINSELIINFKFEEFISKVKEIDKQNLYYFKLIAISNLIVEYSELEIGDGIEKLQEI
                     YMLMSSLVETPNFLQIDLYNDAQIQLGNENKTQIVEFTEFLVSNTDHVKRTYLDDYKEK
                     FLEKYGVNREVQLIELFDSNLGVGSPYGYQHPKNDFWESAPSTIYFNEKEELEYLNKFE
                     KALETGGNIQLYNGEDFFSQAKDKINVGFELFFYVSKVNGKSVLNLTNTGCSKYLGASS
                     GRFSILSDKLEHYHRSITHIVEKNNQASGYHSCEITFLPENLRHANVMRTTNVREKVLS
                     LFTNMDERSQILSDIYIGIDSKNSFYARSFKTGEFLKFYSTNMYNQMMFCNELRFLSEI
                     SQEDHFGIFPWEMVYQKFSHIPRILFKDIIVAPERWRLSGKMLSKDINQIILENNLPIK
                     FYVDNSDNRILINRNNPLDNQLFEDIVRKNSNKNEELCLSECIFDSHLVEKGTTTYVSD
                     IVVPVFVKDEFKEPRCIKEILPEVIPTNVRQKIPFDEWLYFKLYMSADRQEEFLSDIMP
                     QILNFVNLDDGMSFYIRYTDPKFHIRLRIRTRNLYKSFEKIQEIFQLCIQNKLISNIDI
                     STYDREIERYGGLERIPLVEEIFCLDTEIVINSLSLIRQKKLDLSPYDLAIIFNYFYLK
                     SFFKDNNKEIIQFLEFACPEHLDSQNRNKNRNTALIDLYLIKGYLINLLPELSKLCRSL
                     KKLSDSCIHLSDDYTYHIYDSIIHVHNNRLFGIKRENETKIYAIIRGLIISEEYRNRHN
                     HG"
     gene            10349..12145
                     /gene="nsuT"
     CDS             10349..12145
                     /codon_start=1
                     /gene="nsuT"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 157.7; E-value:
                     6.4e-48)"
                     /gene_kind="transport"
                     /note="ABC transporter"
                     /product="NsuT"
                     /protein_id="ABA00880.1"
                     /transl_table=11
                     /translation="MGKFTLKNFFQTILTIPKTIRLIFRIERRYASYLIILNILQSIVP
                     LASLFIYQELINAVLKKGSNIFSVLIVYILMQILLSILSQLNSYISEKFNMNLSYNLNL
                     KLLKKTSSLNLIDFEQADTYNLIENIVQDSTYKPFQLFNAIIGVITGFLSLLTSIIYVS
                     TWNVAVSTFLLIIPVISLVIFMRVGQLEFLIQWERANSEREVWYINYLLTHDFSFKEIK
                     LNGISQYFIDKYGTLKRNFMNQDLNISKKKSLFRGSLDILLNFINGIAIFMMIQSIRSG
                     KLLVGNFVSLIQAITRVNIYSQSMIQNTYIIYNTSLFMEQLFKFFDMEVCNILIQNGKN
                     SKIDKRIDKIKVNNVSFIYPGSVKKTLEAINIEFNRGELVAIVGKNGSGKSTLVKLLSG
                     LYQPSEGQIYYNNESSDVLDLSYYQNNVSVLFQDFVKFELSVRENIGLSEVSSISDSKI
                     KKHIDNFKLNFLTAENNFNLNQRLGTWFNDSRQISGGQWQKVALARTFFKHAGVYILDE
                     PSSALDPVSEKEIFDEFVTRSKDKIALFISHNIMAASRADRIIVMQDGHIIDEGNHNEL
                     ISKSKYYRELYYSENYEEESDG"
     gene            12138..13415
                     /gene="nsuC"
     CDS             12138..13415
                     /codon_start=1
                     /gene="nsuC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: LANC_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1140:Lanthionine synthetase C family protein (Score:
                     311.1; E-value: 1.8e-94)"
                     /gene_kind="biosynthetic"
                     /product="NsuC"
                     /protein_id="ABA00881.1"
                     /sec_met_domain="LANC_like (E-value: 1.4e-61, bitscore:
                     197.7, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDEKKIIVDLSEKVFAKFDEQLKIDEEQQNYDLLTLSSGLPGLVL
                     LSSELTNLTNERKYSVRTDRYINFMIKQMRSYGVLSDSLFSGVSGIGISLLHLVKKHPE
                     YHYLLISFNKYIKYYTLNRIENLEIKNISPTDYDIIEGVSGVLVYLLSQEQVKNDYIIN
                     RIINFLSELSLKNSTLTGFYVVSKNQMSKTEAKLYPLGCLNLGLAHGLAGVGVILSYSK
                     LKGYSNEKSIAAIKKIIMLYEKYELKNYMWKEGLSDIELNKTEKSNLQYEFIRDAWCYG
                     SPGISLLYLYSSLALEDKKLKSKACNILKASIRRSNGLEQSILCHGFSGVIEICLFFKK
                     IYKTNDFDDYIKSLKEKLISDFREDLTYGFNTTAEFENIKTRDNLGYLDGIIGILLTII
                     ELNNLKVTTNWKKPLLLFDDVIKEEK"
     gene            13417..14133
                     /gene="nsuI"
     CDS             13417..14133
                     /codon_start=1
                     /gene="nsuI"
                     /note="putative immunity peptide"
                     /product="NsuI"
                     /protein_id="ABA00882.1"
                     /transl_table=11
                     /translation="MRKFLFCLVVLLGIFTLVACRLVDKKTIEFHDQSFNQFRYKGNEY
                     TIMNQIVDENDLGIMKESFIKTLVIDKKTHKVISKSNSDSISLAYSGLYNHSDGLAISI
                     NDSYYKVSLSSDVKSNNAMLSLDEFKNNLVDGNLAVDSSDCRIVKFNNRKFHISSEIVT
                     DDKLKEFLGVIAVSKTFILNTGQEIPKSELNKIDYFGSNSDRKIEVWDYGEVYLLAEKG
                     NIAVEINNEYRIARIE"
ORIGIN
        1 tacagttgga tcggctggcg atatgatttt ttgggtattg tgtccacaat gcctgagctc
       61 gcaaagaccg aacaaggtct ttagaaaatt tctcgtatga cacatggggt caggtcttac
      121 ccgaaaatgg atgaaagtga gggaaagaga tgtctgaaca gtaccgtgac attcgaaaag
      181 aggtaaatct aaccgctgat gagctaaaac ggattgaaaa tatgatggag gttggtaatt
      241 atcgacaatt ttctctcttt ggcagtttct aaagttagaa taggttatct acgtttaaag
      301 taggatgaat ctaacgacaa atataaaact agaataagtt gaacatgaga ttgaaacttc
      361 tcaaaataac ttaaataaat ttatagatgt ttatgaatat tttttagaat attggaaaat
      421 ttgtagacaa tctaagtagt attgatttga acaaagagaa gtaaaaatta gaattcaaag
      481 aaaatctatg aagaaagcat tctttgtgaa ggtattgata tctgataatt aattctgaag
      541 aaattctgaa attaatttga taaaatgcgc taaaaaacaa aggagttagt attatgaaga
      601 ggaaatgccc tttattaaaa atttttttga cattggtaat tgcaatattg tttaatatct
      661 ctatatcttc agaagtaaat gctagtgaaa acaagggaac catatataat gttgtaatta
      721 gagaaaataa atcaattgaa agtgtaaaaa atatttttca agagcaagga attaaggtta
      781 ttgataagat tcctgaaata aattttttaa ttatttatac tgaaaaaagt gaagatacta
      841 taaaaaaaga aaacactgat tatattgacg acatagttag agataatact atgactgtaa
      901 agcccaatat tacctatttg tatggagaaa aggtctatac aaatactaat ttagattttt
      961 gggggaatca gtgggatatg caaaaaagta ttagtactgg aagtaaattt aaacataagt
     1021 cctctggtaa agcaacaatt ggtgtaattg attcaggtat tacatatgat aatccagata
     1081 ttttttcaca tattatttca gtacaaaatt tcacatcaaa tttggaaact ggagtagttg
     1141 ataagcaaaa tgttgttgat aaaactggcc atgcaacttc tgttgtgggg caaatctcat
     1201 caagtgggga atatttggga atagcacctg aaatgaaaat acgaatgtat cgtgtttttg
     1261 atgaagggaa tgctcaagac caatggattc tcaaagcgct cattcaagca gcaaaagatg
     1321 atgttgacgt gattaatctc agtttaggag aatatttatt aagaggttcc actgatgaag
     1381 atgataatac agctttaatc aatatctatc agcgggcaat taattatgct cataatcaag
     1441 gttccgttgt tgttgcgtca gtaggtgatg aagggattga tttacagaat caagataaat
     1501 taaaaacata ccttggtggt ttaagaggaa aagattatag tcaagtaaat ggaactgtac
     1561 aagatattcc agctgagttg gacaatgttg ttacagttgg atcagtagat gataacgatc
     1621 ttgtatctag tttctcaaat aaagggaaag acagtattga tatttatgca acgggaggag
     1681 gaagtcgaga actttctcaa ataggatacg aaaaatgggt atctgataga ttattcgaaa
     1741 aagaatggat tctcgttcca acgcttgaag gaaaatatac atacgcatat ggaacatcca
     1801 tttcaactcc taaagtctct gcagctttgg gattgattat agaaaagtat aatttgaagg
     1861 atcaaccaga tgaagccatc cgaattttat atgataattg ttttcttagt aataatggaa
     1921 ataatggaca aattaggtta ctaaatatta caaatttttg caataatagt gtaaaataaa
     1981 agcatgagat tatcgaggta tctaatatgt ataagatttt agtaattgat gatgattttg
     2041 aaattttaaa attgatgcga acaattttag aaatgaaaaa ctataatgtg acaacttatc
     2101 aagaggtcaa acttcctatt gaaatgagtg attttaaagg ctttgattta atattgcttg
     2161 atgttatgat gcctgatatt gatggtttgc aaatttgtaa acagattcgt gaatcagtat
     2221 cctcgccgat tatttttgtc agcgcaaaag actctgaaga tgacattgtt tcgggattga
     2281 atctaggtgg ggatgattat attacaaagc catttagtgt taatcaacta gttgccaagg
     2341 ttgcagcgca tttgaaacga gaagagcgcc ataggaattc aaaatccaat gaaaatgttg
     2401 ttcgagaatt gttgccaata acgttttacc ttcaagaaaa aattgtgtgt attaatggtg
     2461 aagttatctc tctaacctgt agagaatatg acattttaga cttattagct agcaaaaaaa
     2521 ataaggttta tacaagagaa gaaatctata aacgtgtcta tgatgaagaa tcggatgcat
     2581 tatttcgttc aatttcagaa tatgtttatc aaattagagt taaattttct ccatatggga
     2641 ttaatcccat caagacaata aggggaattg gatataaatg gaataatgaa aaagtattca
     2701 attagtaagc gaattttaag tactattatt gaaatagtaa ttggtactat tgcaaatctt
     2761 tttttgttac tcgttttgag ccatattctt gttttgacag gtcagcttaa tacatatgga
     2821 acagactatc atatctcagt gaaagataaa agatctattg acagtatcaa aaataagata
     2881 gatcatacgg tgtttgatta tgtactgttt gatagtaaaa ctggtaaact agtctctgga
     2941 aaatatcaaa aacaggattt acaacattat aaagaagttt ttgatactgg aagttcaggc
     3001 agcgacggtt ctatattgta tacagattat ggggattcaa actttacttt tgttgttaga
     3061 agaccgacaa ttcctgaatt tgttaaccca aattttcgac atgtttctta taattcagtc
     3121 tcttatttgc tttttatggg gctagaatta ctactcattt tttcctctgt tattaggctt
     3181 ttaaaagaat ttaccaaaaa ctttcgtttg attgaaaaaa tatcacttaa catggggata
     3241 ccagaaatgg atattgaacg taaagaaact gaaatgcgag aatttaataa tattttactg
     3301 accctctacc aaaaagatga agagttgaca cagttattag aaatggagaa aaaagataag
     3361 gaggatttgt catttcaagt tgctgcatta gcacatgaca ttaaaacccc tttaactgtt
     3421 ttaaagggaa atatcgagtt gcttgagatg actgatttag atgaaaaaca attagatttt
     3481 atgtcttcaa tgaatcacag cgtggatata tttgaaaagt attttaattc aatgattaat
     3541 tacgcaaggt tactaattga agataaagat tatcaggaag acattgattt atcagaattt
     3601 cttgatgaat tatcattaga atgtcgagat attatgactt caaaaaaaat tgactttcaa
     3661 atagttaatg aatctcaagt tgaagtgatt aaaggaaatc gactcaactt agatagggct
     3721 ttgctaaata tcctatctaa tgctcttaga tattgttctg aaaagggtga ggtggtgctt
     3781 tccatatctg agagccctaa ttttgtcagt tttgaaattt ggaataatgg aataccattt
     3841 acagaggaag cactaaagga tggtggaaaa cttttttaca cagaaaataa agggagaaat
     3901 gataagcatt acggaatagg actttcattt gctcaaaaga tagctatgag acatcaaggt
     3961 caattagtac tctcaaatcc acaaaaaggt ggggcacaag tcactttatt aattaaaaat
     4021 tttcagaggt agcaactaga aagtaatgct accttttttc tgaacaaaaa ctgaattttc
     4081 ttttgtatac tatactcaat caaagagatg gaggtagaaa aaaatggaag tcatcattaa
     4141 aaacttaaga aaaagctata aagataagga agttttaaaa aatgtttcct tcagaataga
     4201 agaaggtact atttgtagtc tactaggaat aaatggcgct gggaagtcca ctattatgaa
     4261 aatccttttt agtttggaaa gtgctgatag tggagaagtg atttttaatg gagagtacaa
     4321 aacgcctgag gggactgata gatttgatat tggagcgttg attgaatctc cagctatcta
     4381 tctgaatctt tctgcattcg ataatctaaa gacacgtgcc ttgctacatg gtatctcaga
     4441 cgaaagaatt ctggaagtac ttcatttggt tggtttagat ggtactggca aaaagaaagc
     4501 cgcacatttt tcattgggaa tgaaacagcg acttggttta ggaatggcaa ttatcacaaa
     4561 tcctgattta ttaattttag atgagccgac caacggttta gatccagatg ggataaaaga
     4621 attgctaaat ttaattatga ctcttaaaaa atcaggaatg accatacttc tatcaagtca
     4681 tcaattaagt gaagtcagca aagtcgcaga taaaatagtt attctgaata aaggtgagat
     4741 tttttatgat gatataaata aaggtacgaa tgacttagaa aatctattct ttagaattgt
     4801 ccatgggggt gaataaaatg attaatattt tcaaatctga atggcttaaa caaaaatcca
     4861 gttctgataa aaaattacta atctttatac cttttctagc gatttttatt gcttttatac
     4921 tcgctggacc tattaatcta gaaagtttct caatttattg gtgggaaggg atttttctat
     4981 acaccctttt tggactgtta tttttagccg attttaatgc agaggaaaat gcaggttcat
     5041 ttcaaaatat aagttttggt agaaatacat ttactattta tatagcaaag atactattaa
     5101 agttgaagga tattttgatt tcaactttta ttttcacaat aatattttac tgtatctcat
     5161 gtcttttctc cggtatgatt acagtagata ttgccaaaga ttttatctgc ttattactca
     5221 ttggtttttc ttctgcttgg actcttccat tattatattt tctgtcaaaa tgggtaaact
     5281 cctacctttt attggctgtg aattccctta tttgcttgtt ggttgctcca cttattgctc
     5341 agacatcgat ttggtttata tttccttata cctatcatta taaaattgct catgcttttt
     5401 tgcatttgaa accatcaggt gatttagata tagcagcaag tgggattggt atgatgacaa
     5461 ttttagtagc aatgggccta tcaatcttag tttttgtgac tttccttagt ttgttgaaat
     5521 ggaggataac aaatgaacaa aattataaaa agcgaattac ttaagtggaa gggttctttt
     5581 gctttaaatc ttattttggt tttagcaata atccaacttt tcacaattcc tctttattta
     5641 cactttacta ataatttagt tttaattgaa aatatcattt ttttacctat gctaggttat
     5701 tgtatattag catcaattct ctctatatta ctttatgaac aagaggagaa agcaaattct
     5761 tttcaaaata ttaaaagtga aaaaaataga tcctccattt ggggaatgaa actaatttcg
     5821 acagatttac taatggtttt gttgggtgtt ccagtttgga tagtagtagg gtttggtttt
     5881 gatagacttt cctattatgc atatgtaggc gtgattactt ggttattatt agttttactg
     5941 aatcattttc atatgttact tagtctaatc atgacaaaag taggaaattt agtaattgct
     6001 tttattgagt gtttgtttat tatatttgcg acaaacaaag tatttttaaa aagcttttgg
     6061 ctaccgattg ctttacccgt taatctcata ttggaatttg gaggaaacaa aattcttatg
     6121 atctttgcgt atttagttgg ttttattatt ttgttgtatt tttgtaattt aaccgttata
     6181 actgagtttg aaaatcaaaa aaagaaaaaa ctatataaga aatagtaaat tagcgatact
     6241 agccacctaa aatgagacac aagcaaaaca cttctgttga aatctagtaa actagaaaca
     6301 ggagtatttt gttatggtca aaaaagcgta ttcagtggaa attaagttaa cctatattga
     6361 aatgaaaaag gcaagtaaat ctaataaggt cattatggag acctttggta tcttgaatat
     6421 tagtcaggca aagacctggt agcgatagta tgtgaatagt gtactattac aaagcgacca
     6481 aggttctgtg tttacctcaa aggcatactt tcaggtttgt atgaacagaa aaaggcatca
     6541 cccgctcaat gtccctttag gggacaccag cagataattc ctgtaccgaa tggttccatt
     6601 ctgtcttaaa gactgaaatc ttatatctcc ataactggag aaccttaacc aaagatagta
     6661 taacagatat tgccaaagca ttataacatt ttcataatgg aattagaatt aaatagcatt
     6721 taaacaactt ttctccggtt caatacagaa aattagtcgc ctaaaaaatg ttcctctgct
     6781 gtctcattat caacatgcag tttcagttgt ttataatttt ttgtttagtt cctaaaaata
     6841 ttaaataaat attgcattta tttttacgaa aaatttgtca atctttctaa ttatgaacaa
     6901 attctgaact aagtatttta caatatactt gttcgaacaa agaaataaca attggaggaa
     6961 atttattatg aacaatgaag attttaattt ggatctcatc aaaatctcaa aggaaaacaa
     7021 ctcaggagct tcacctcgaa taactagtaa atcattatgt actcctggat gtaagacggg
     7081 tattttgatg acttgtccac taaaaactgc aacctgtggt tgtcattttg gataaaattt
     7141 gatttgtttg gtaactatct tgattcaaat cctgactgag aatgaataga agtctctatc
     7201 ccattagaag cgagtttttg ctcattacta gtagtatttg ttgaggatat tacctcttgg
     7261 atgaacaagt ctattgttaa aataaaggta gtggatagat agcttgctac ctttattttt
     7321 ataagttcct aaatttatat ttagagattt ataaaaataa tctagaaaag aggaaaagta
     7381 atgaaaaata gatatattgc gaataatttt ttggtccgta atacaattac tgagacacat
     7441 gagttgttgg aatgtgatta taattcattt ttagaaacta taagagagaa taagattttc
     7501 caggagcaac tatttgtagc cagcaggagt ttatatgaat cacttcaaaa atattatagt
     7561 ggtgattcaa tgaaaaggaa aaaaatcaac caattatcag agtctgtata taaatattat
     7621 aagcgtagta aggagcgctc aacaccattt ggtttattta gtgaaacttc aataggttca
     7681 ttttcttcta cagagaaatt aaacttgaat ggaaaaacat taaaaaaagt actcttggat
     7741 tcagagtggt taatacgtct agtttttaag attgagaagg aatattctcg agaattagcc
     7801 tataaaataa attcagccaa ctatcaattt ggagatagag ttgttcaatt gttttctata
     7861 aatgatacta aaactgaaga agtcaacata aagttcacta aagtttatca acttatttat
     7921 gagctttgtt gtgataaata tgtatatttt aactgtatta ttgaaaaact tgttgaatcc
     7981 tatggtgaag agtatcgaga tattgcaaca tcatatatca tgagtttgat tgatagtcat
     8041 tttctaattt caaatatcaa ttcagaatta ataataaatt ttaagtttga agaattcatt
     8101 tctaaagtca aagaaatcga taagcaaaat ctttattatt ttaaactaat agcaattagt
     8161 aacttaattg ttgaatattc tgaattggaa attggggatg ggattgaaaa gcttcaagaa
     8221 atctacatgt taatgtcatc attggttgaa actcctaatt ttcttcaaat tgatctgtat
     8281 aatgatgctc aaattcaatt aggtaatgaa aataaaacac agatagtaga atttactgaa
     8341 tttcttgtaa gtaatacaga tcatgtaaaa cgtacttatt tagatgatta taaagaaaag
     8401 tttctagaga agtacggagt aaatagagaa gttcagttga ttgagttgtt tgattctaat
     8461 ctaggagtag gttctccata tggatatcag cacccaaaaa atgatttctg ggaatctgca
     8521 ccttctacta tatattttaa tgaaaaagaa gaattagaat atctcaataa gtttgaaaaa
     8581 gccttagaaa caggtggaaa tattcaactt tataatggag aagatttttt tagtcaagcc
     8641 aaagataaaa tcaatgtagg atttgagttg ttcttttatg tcagtaaagt aaacggtaaa
     8701 agcgtactaa acttaactaa tactggttgt tctaagtatc taggggctag ctcaggtagg
     8761 ttttctatcc tatcagataa attagaacat tatcatcgaa gtatcactca cattgttgaa
     8821 aaaaataatc aagcatcagg gtatcattct tgtgaaataa cattcttacc agagaatctt
     8881 cgtcatgcta atgttatgcg tacaacaaat gtcagagaaa aagtattatc actttttaca
     8941 aatatggatg agagaagtca aattttatct gatatatata ttggtattga ttctaagaat
     9001 agtttttatg ctagaagttt caaaactgga gaatttttaa aattttatag cacaaatatg
     9061 tacaaccaaa tgatgttttg caatgaatta cgatttttaa gtgagatttc ccaagaagat
     9121 catttcggaa tattcccttg ggagatggtt tatcaaaaat tttctcatat tcctagaatc
     9181 ttatttaagg atattattgt tgcacctgaa cgttggaggc ttagtggaaa gatgctttca
     9241 aaggatatta atcagattat tcttgaaaac aatcttccaa taaagtttta tgttgataac
     9301 agtgataatc gaatattaat taaccgtaat aatccgctgg ataatcaact ttttgaagat
     9361 attgtaagaa aaaatagtaa taaaaatgaa gaattatgtc tatcagagtg tatttttgat
     9421 tcacaccttg tagagaaagg aactacaaca tacgtttctg atattgttgt cccagtattt
     9481 gttaaggatg aatttaagga acctaggtgc ataaaagaaa tattaccaga agttattccg
     9541 acaaatgtta gacagaaaat cccatttgat gaatggctct actttaaatt gtatatgtcg
     9601 gcagataggc aagaggaatt tctttcggat ataatgcctc aaattttgaa ttttgtaaat
     9661 ttagatgatg ggatgagctt ctatattaga tatactgatc caaaatttca tatccgacta
     9721 aggataagaa cccggaattt atataaaagt tttgaaaaaa ttcaagagat ttttcaactt
     9781 tgcatccaaa acaagttaat atcaaatata gatatttcaa cgtatgacag agaaatcgaa
     9841 agatatgggg gattagaaag aattcctttg gtagaagaaa ttttttgttt agatactgaa
     9901 atagtcatca attcattaag tttaattcgg cagaaaaaat tagatttatc cccatatgat
     9961 ctggcaataa tatttaacta cttttatctc aaatcatttt ttaaagataa caataaagaa
    10021 ataatacaat ttttagaatt tgcctgtcca gagcatttag acagtcaaaa tagaaataaa
    10081 aataggaata ctgcattaat tgatttgtac ttaataaagg gatatttaat aaatcttctt
    10141 ccagaactat ctaaactatg tcgaagctta aaaaaattat cagacagttg tattcattta
    10201 tcagatgatt atacatatca catttatgat agtatcattc atgttcacaa taatcgctta
    10261 tttggtataa agagagaaaa tgagactaag atttatgcaa taattcgcgg gttgataatc
    10321 agtgaagaat ataggaatag gcacaatcat gggtaaattt acactaaaaa atttttttca
    10381 aacaattttg acaattccta aaacaataag attaattttt cgaatagaaa gaagatatgc
    10441 tagctatcta ataattttaa acatattaca atcaatagtt cctttagcaa gtttatttat
    10501 ctatcaagaa ttaatcaatg ctgttttaaa aaaaggaagt aacatttttt cagtattaat
    10561 tgtctatata ttaatgcaaa tactgctatc aatactaagt cagttgaatt catatataag
    10621 tgagaaattt aatatgaact tgtcctataa tctcaatttg aagttgttga aaaaaacatc
    10681 atctctaaat ttaatagatt ttgaacaggc agatacatac aatctcattg aaaatattgt
    10741 acaagatagt acttataagc cgttccaact tttcaatgct atcattggtg tgattacagg
    10801 gtttctatct ttattaacaa gcattattta tgtgtccact tggaatgtgg cggtatcgac
    10861 ttttttgctg ataattcctg taatttcctt agtaattttt atgcgtgttg gtcagctaga
    10921 atttttgatt caatgggagc gagcaaattc ggagagggaa gtttggtaca tcaattatct
    10981 tcttactcat gatttttcat ttaaagagat taaacttaat ggcattagtc aatatttcat
    11041 tgataaatat ggaacattga aaagaaattt tatgaatcag gatttgaata tttctaaaaa
    11101 gaaatccctt ttcagaggaa gtttggatat tttattgaat tttattaatg gaatagctat
    11161 ttttatgatg atacagtcaa ttcgaagtgg taaactttta gtgggtaatt ttgtaagcct
    11221 tattcaggca ataactcgtg taaatatata ctcacaatct atgatccaaa atacttatat
    11281 tatctataac acaagtttat tcatggagca attatttaaa ttttttgata tggaagtatg
    11341 taatatctta attcagaatg gaaaaaactc aaaaattgat aagcgcattg acaaaataaa
    11401 agtcaataat gtctctttta tttatccagg aagcgtaaaa aaaactttag aagctattaa
    11461 tattgagttt aataggggag aacttgtagc aattgtgggg aagaatggtt ctggtaagag
    11521 taccttagta aaattactat caggattata ccaaccttca gagggccaaa tatattataa
    11581 taacgaatct agtgatgttc tagacttgag ttattatcaa aataatgttt cagttctttt
    11641 tcaggacttt gtgaagtttg aattatctgt gagagaaaat attggcctaa gtgaagtaag
    11701 cagtatatct gactcaaaaa taaagaaaca tattgataac ttcaaattaa atttcttaac
    11761 cgcagaaaac aatttcaacc ttaatcagcg tttagggacc tggtttaacg atagtagaca
    11821 aatttcagga ggacaatggc aaaaagtagc attggctagg acttttttca aacatgcagg
    11881 tgtatatatt ttggatgagc caagttcagc gcttgatcct gtttcagaga aagaaatatt
    11941 tgatgaattt gtaacccgtt ctaaagataa gattgctttg ttcatatcac ataatataat
    12001 ggctgcgagt agagcagata gaattatagt tatgcaagat gggcatatta ttgatgaagg
    12061 aaaccataat gaacttataa gtaaatccaa atattatagg gaactttact attcagaaaa
    12121 ctacgaggag gaatctgatg gatgaaaaga agatcattgt tgatttatcc gagaaagtat
    12181 ttgctaaatt tgacgagcag ttaaaaatag atgaagaaca acaaaattat gatttattga
    12241 cattgtcctc tggtttacct ggattggtat tattatcctc agaattaaca aatttaacta
    12301 atgaaagaaa atattcagtt agaactgata gatatattaa ctttatgatt aaacaaatga
    12361 gaagctatgg tgttttgtcg gattctttgt tttcaggagt ttcaggaatt ggaataagcc
    12421 ttcttcattt agttaaaaaa catccagaat atcattactt attgattagt tttaataaat
    12481 atattaagta ctatacgtta aataggattg aaaatttaga aattaagaat atttcaccaa
    12541 ctgattatga catcatagaa ggagtatctg gagtattagt ttatctttta tctcaggagc
    12601 aagtaaaaaa tgattatata ataaacagaa ttatcaattt cttatctgaa ctatcactaa
    12661 aaaattctac tctaacaggc ttttatgtag tatcaaaaaa tcaaatgtcc aagacagagg
    12721 ctaagcttta tccgttagga tgtcttaatt taggattggc gcacggtctt gctggtgttg
    12781 gtgtaatatt gtcatattca aaactaaaag gctattctaa tgaaaaatcc attgcagcaa
    12841 tcaaaaaaat tataatgtta tacgaaaaat acgagttaaa aaattatatg tggaaagagg
    12901 gattaagtga tattgaacta aataaaacag aaaaaagcaa tttacagtat gaatttattc
    12961 gagatgcgtg gtgctacggt agcccaggaa ttagtttact atatctgtat tcaagtttgg
    13021 ctctagaaga taagaaacta aaatctaaag cttgtaatat cttgaaagca tccatcagaa
    13081 gaagtaatgg tttagaacaa tctattttat gtcatggatt ttctggagta attgagattt
    13141 gcctattttt taaaaaaatt tataaaacaa atgattttga tgactatata aaatctctta
    13201 aggaaaagtt aattagtgat tttagagaag acttgacata tggttttaat actaccgcag
    13261 aatttgaaaa tataaaaaca agagataatc ttggttattt agatggaata attggaattc
    13321 ttttaacaat tattgaacta aataacttaa aggttacaac aaattggaaa aagccactct
    13381 tactttttga tgatgttata aaggaggaaa aatagcatga ggaaattttt attctgctta
    13441 gttgttcttc ttggaatttt cactttagtt gcatgtagat tggtagacaa aaaaacaata
    13501 gagtttcatg atcaatcatt taatcaattt agatataaag gaaatgaata tacaatcatg
    13561 aatcaaatcg ttgatgaaaa tgacctaggt attatgaagg aatcttttat taaaacacta
    13621 gtaattgata aaaagaccca taaagttatt agcaaaagta atagtgattc aatatcatta
    13681 gcatatagtg gactctataa tcatagtgat ggattagcta tttccataaa tgattcttat
    13741 tataaggttt ctttgagttc ggatgtaaaa tctaataatg caatgctgag cttggatgag
    13801 ttcaagaata atttagtaga tgggaatctt gcagttgatt catctgattg taggatagtc
    13861 aaatttaata ataggaaatt tcatatttca tctgagattg taactgatga caaactaaaa
    13921 gaatttctag gagtaatagc agtttcaaaa acatttattt tgaatacagg acaagaaatt
    13981 cctaaatcag aattaaataa aatagattat tttggatcta attctgatag aaaaatagaa
    14041 gtttgggatt acggagaagt ttatttgtta gcagaaaaag gtaatatagc ggttgagatt
    14101 aataatgaat atagaatagc acgtatagaa taaattatag attatctatt gcttatataa
    14161 caaaatatct tattgttacc aataaataga aacttattaa aatttagaac agcaagttca
    14221 ggagagtttg ctattttttg tcaaatgtaa tttttttgga agaaaagcat cctattttca
    14281 ctcttataac gatgaggact tctcaaactg aataaaaagt gagaaagatc tatgattgag
    14341 ataaggaacc ctcctcttaa gacttagaaa agtcattaaa ggaggatgaa atgaaatatt
    14401 atgttcatgt tagaggtagt aaaattcctg taacagaagt agtttaaaaa agttattgtc
    14461 aattgataat gcacgagaaa tatcttaata gtaaagatag gaaattcggt gtattaccat
    14521 cttcatcatt tgaagtacat tgagtgtgtg agactgtttg aaaaaggcaa gtcatccaag
    14581 tttatttgaa gatagatatt tcctgtgact aaccacaatt tatgattggt tattttgcat
    14641 gctataaaaa tgacacgtga ggtcatttga tgtaagaatt cataaagact tctcattttc
    14701 taaagtgaaa tattgtataa agaaaatagg tggtttttgt attactataa agacagatta
    14761 aacagtcttt tatctttttc tattgtctaa catcggaggc tcctaatgaa atttatggtt
    14821 aagtcttgga ctttgggtcc ggccacctcg tcttttttct taagtttaat ttttaatatt
    14881 ttggggattg tcatacttta tcattatttt gcttcttttt atcgcagtta atgggagctt
    14941 tttgtagctg cagtcatgtt tgagatttgg ctgataaatt gcaagattgt ctctcattat
    15001 tgaattgtct aattgaaatt aatgaggttg gtgtccgaat ttcaaggctg ttaattactg
    15061 aagaagaggc ttagggacgc cagacttctt taaacgataa atttctatct tatcctcatt
    15121 acctaatttc atacaaactg ccctcctatt attagatttt atgtctaaca ataggagggc
    15181 agttcaaagt gaattgaggt tttttgtatt tttgcttgct agactaacaa taaggctaat
    15241 ccgataacca taaaggctac cattttataa ctttcattga ttagaattaa tgtgagtgat
    15301 ttttgttcaa agaagtagtt ttttaatcct gaaagaacag agatgattcc tataacaagg
    15361 gcattcaatg gcgttgcagc gtgaatagct gacaagaaga gtattgttag gatactgatg
    15421 ataacttcaa caattaaggt catcatcatt tcttttttgc catcaccatt tttaccgatg
    15481 tcttcgggat taatacccat ggctttgatc catgcttttt ggaagagcat gccataccaa
    15541 agaccgccaa tcatgaagtt aattactcct gttacaattg ctacaataag tgtcattttt
    15601 ttctcctatg tgttttcgtt gggggggatt actggttttt gacctgatat gtt
//
