LOCUS       BGC0000540             11692 bp    DNA     linear   BCT 28-FEB-2013
DEFINITION  Paenibacillus polymyxa OSY-DF PaenA (paenA), PaenP (paenP), PaenB
            (paenB), PaenC (paenC), PaenI (paenI), PaenT (PaenT), AgrB (agrB),
            AgrD (agrD), histidine kinase (agrA), AgrC (agrC), and PaenN (paenN)
            genes, complete cds.
ACCESSION   BGC0000540
VERSION     BGC0000540
KEYWORDS    .
SOURCE      Paenibacillus polymyxa OSY-DF
  ORGANISM  Paenibacillus polymyxa OSY-DF
            Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae;
            Paenibacillus.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 13:48:48
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..11692
                     /db_xref="taxon:1156938"
                     /mol_type="genomic DNA"
                     /organism="Paenibacillus polymyxa OSY-DF"
                     /strain="OSY-DF"
     protocluster    1..11692
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[8230:8644]"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      8231..8644
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="1"
     protocluster    1..11692
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[1291:5657]"
                     /cutoff="20000"
                     /detection_rule="((LANC_like and (Lant_dehydr_N or
                     Lant_dehydr_C)) and not (YcaO or TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="2"
                     /tool="antismash"
     proto_core      1292..5657
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="((LANC_like and (Lant_dehydr_N or
                     Lant_dehydr_C)) and not (YcaO or TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="2"
     cand_cluster    1..11692
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(TIGR04223 or AgrB)"
                     /detection_rules="((LANC_like and (Lant_dehydr_N or
                     Lant_dehydr_C)) and not (YcaO or TIGR03604))"
                     /kind="neighbouring"
                     /product="cyclic-lactone-autoinducer"
                     /product="lanthipeptide-class-i"
                     /protoclusters="1"
                     /protoclusters="2"
                     /tool="antismash"
     subregion       1..11692
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000540"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..11692
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="cyclic-lactone-autoinducer"
                     /product="lanthipeptide-class-i"
                     /region_number="1"
                     /rules="(TIGR04223 or AgrB)"
                     /rules="((LANC_like and (Lant_dehydr_N or Lant_dehydr_C))
                     and not (YcaO or TIGR03604))"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            118..279
                     /gene="paenA"
     CDS             118..279
                     /codon_start=1
                     /gene="paenA"
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /note="paenibacillin prepropeptide"
                     /product="PaenA"
                     /protein_id="AFS60100.1"
                     /transl_table=11
                     /translation="MKVDQMFDLDLRKSYEASELSPQASIIKTTIKVSKAVCKTLTCIC
                     TGSCSNCK"
     CDS_motif       118..186
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence: MKVDQMFDLDLRKSYEASELSPQ"
                     /locus_tag="paenA_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       187..279
                     /prepeptide="core"
                     /locus_tag="paenA_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class I"
                     /score="14.70"
                     /molecular_weight="2943.6"
                     /monoisotopic_mass="2941.6"
                     /core_sequence="ASIIKTTIKVSKAVCKTLTCICTGSCSNCK"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MKVDQMFDLDLRKSYEASELSPQ"
                     /leader_location="[117:186](+)"
                     /alternative_weights="2961.6"
                     /alternative_weights="2979.6"
                     /alternative_weights="2997.7"
                     /alternative_weights="3015.7"
     gene            313..1287
                     /gene="paenP"
     CDS             313..1287
                     /codon_start=1
                     /gene="paenP"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075:alkaline serine protease, subtilase family
                     (Score: 135; E-value: 7.6e-41)"
                     /gene_kind="biosynthetic-additional"
                     /note="peptidase"
                     /product="PaenP"
                     /protein_id="AFS60101.1"
                     /sec_met_domain="Peptidase_S8 (E-value: 1.3e-24, bitscore:
                     76.4, seeds: 43, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGYPYLHYGDCMIKPNIYNWHVARLTDHFSCGATVGQPLTILFID
                     TGVDKSHSEIQGNLVEAECKSFVPYEPCISDYVGHGTQMVSSVTGKYILKGICPFARVV
                     VYKITDKKGISKLEWLYRALEEGIAKGYPIINVSYRTEYEVDLFEFNRFQALVDCANAQ
                     GIWVVCSAGNDGRDITQEYRVPANLKGVYPIGSTNKNGDISGFSNTLNDQFFAPSGDEY
                     EINYNNDWVMVAKSSFDTEDYLYEVLGFDKAYTVGIGTSIASALFSGVVASLSGELIAQ
                     GMNAGRTEMECLLRQGARLIDVQRNYYEVSLAGSIRALRQMRG"
     gene            1292..4375
                     /gene="paenB"
     CDS             1292..4375
                     /codon_start=1
                     /gene="paenB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_N"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lanthipeptide_LanB_RRE"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1155:Lantibiotic dehydratase domain protein (Score:
                     647.8; E-value: 4.5e-196)"
                     /gene_kind="biosynthetic"
                     /note="lantibiotic dehydratase"
                     /product="PaenB"
                     /protein_id="AFS60102.1"
                     /sec_met_domain="Lant_dehydr_N (E-value: 2.4e-114,
                     bitscore: 372.2, seeds: 73, tool: rule-based-clusters)"
                     /sec_met_domain="Lanthipeptide_LanB_RRE (E-value: 1.8e-14,
                     bitscore: 43.9, seeds: 20, tool: rule-based-clusters)"
                     /sec_met_domain="Lant_dehydr_C (E-value: 5.2e-27, bitscore:
                     84.7, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLENFEISENYMYRKTLLGMEHYTQIFDHEQLDDDEYLDRLVRFV
                     QQQGLIDSIYVSSPSTYAALQSLEQGRTLKSKKKRNLILSLSKFISRAICRPTPFGLFA
                     GVGISRFGKTGGAGEHVVWATPDYSLIYRLIGLFHGDADVRNELRLSVNNTSYIDHHRL
                     VISYQTLFAADQEERSTENISILKTPVIDFIMKILDKEQQTFLQLMQAIKDAFGVDDTI
                     STDFLQQLIEQGFLVSEMIPNPRDTAPLEHLIRTLKLLAAPKAAYYSDILERVGQAIQD
                     ITVAAVKREGIEAVRKLVQLILPDYQGDLVKIDVRLNQVNEILLSDSDRQSLAQIASIL
                     SMLSTYRKEDTLSDYRQQFTEKYGVYEEVPLLQLFSKTLGLGKPEEYKSHYQNGDKYRH
                     KDEGRFRQLKALIAEWQTEALVNHSDIVLDEVKIQQLSELSLENVHVSFDLYYSCMENE
                     QGEVTFYLNNRAGSLEACQSFGRFAYMFDKDSRDEIFNFVRNPKDQKAGESGAEISFYP
                     FSPKITNIMTAGPTPDQSMDMATFPENRKLDVSQLLVGVEDSGYFYLKHRETGELVFPK
                     LSSMYNTELAPYLIRFLNDINLQYQTGWSHLEEYLYDSSFTPRVTYKNIIICPKKWTLY
                     ADKQLQSEEQFLAFLEDWIIKYNIPRYVYLVELDNKLLLDLDRHHHRKLLHKEYTRNPE
                     GKAAVIMETEKELFASANRYMEECVFFCKSPMFQYLPIDRLSAQATDKSLNKVLSSSVN
                     AVYYPGSDWISLQVYYNQEMLTDLLGSAFKTFMDNSAEYFIDQVFFVQYADKEPHIRLR
                     CKMSGSVEHQRFTALNYVFDFLNTVTSSGLVSSYSVVPYRPEILRYGGAAFIEQAERLF
                     AIDSYLVSGYYAGKRSINATDQMLFCCTGLFEVVNCAYPLAEDQLKAMAAIIDPKKFRQ
                     EYREHKEALFQRIQQTRSISLPASDTGDRLWQYAAYFTAIREADDRTNYYDDIMFSIMH
                     MFCNRVFGLDREKEELALHLCYYSLEDYLQVSKYHVAY"
     gene            4386..5657
                     /gene="paenC"
     CDS             4386..5657
                     /codon_start=1
                     /gene="paenC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: LANC_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1140:Lanthionine synthetase C family protein (Score:
                     248.3; E-value: 2.1e-75)"
                     /gene_kind="biosynthetic"
                     /note="lantibiotic cyclase"
                     /product="PaenC"
                     /protein_id="AFS60103.1"
                     /sec_met_domain="LANC_like (E-value: 2.5e-52, bitscore:
                     167.3, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAVKQVPGNLSEIVRQSAEIYKDIDTVTLLIKKHAMHDYPEMELI
                     SLASGYPGISLLLDKLDEVYPGEGWDALAFKYNQEIVRLIGNIPFHSLSLYTGLAGVIY
                     LFNECSRGSTRYTNVLSQTLDMFLSSYGEFYEETRGKLEDSLIEDSDLDLISGWTGIAA
                     ALMEVKERQDRYAGKLDAVLLKILQLITDAVGKLRHFGELPEQTIYYNLGMAHGITGAI
                     NLLAVAHLRRYPLTGLREVLEVAKTFLLGNIREWNGTIVIPNMFTPDESAETSRNPGYH
                     RDAWCYGTPGASVAMFRLGLALEDKALQQQALQLFNTVYSRPDSLRKVISPTICHGYAG
                     LLLIQHHFSRTAAIPFKNDFLHRMLSYRNTQEAVPFTDLEGSVDDPLSLDKVGLLDGCA
                     GVLLTLLTCDNENLSTWKQMLVFS"
     gene            5673..6230
                     /gene="paenI"
     CDS             5673..6230
                     /codon_start=1
                     /gene="paenI"
                     /note="putative immunity protein"
                     /product="PaenI"
                     /protein_id="AFS60104.1"
                     /transl_table=11
                     /translation="MFILLKEIITVMVGLITDFLSFQLDAYFYIWFLVLFISSLAATLV
                     QRSEGTITYSTAECVGINVLVVVLYLVIGTILARYFASFEDQQIVAGDKIMLYKISFML
                     TSAVIIVDIILDRLKRGRKFSYSLEFLAGSAVCFLLFQLSNQTDWIGTKLSLSYIVMVA
                     IAAGVYTMAMKFLLEYENHKEK"
     gene            6245..8038
                     /gene="paenT"
     CDS             6245..8038
                     /codon_start=1
                     /gene="paenT"
                     /gene_functions="transport (smcogs) SMCOG1288:ABC
                     transporter related protein (Score: 333; E-value:
                     5.7e-101)"
                     /gene_kind="transport"
                     /note="ATP-binding cassette transporter"
                     /product="PaenT"
                     /protein_id="AFS60105.1"
                     /transl_table=11
                     /translation="MELNMESTAYKKIGEEKQTAHSLLELIKIARWPKLALAWSIVFTL
                     ISAAFSLVLPIITGKLVDKISAMELTIQTAAVLISLFLAGGILNAVSSLLLRYVGSKFF
                     QELRLKIWEKIIHLPVNYFDNNENGKIISRMTNDLAIVSNFLAITLPSILADVLMVTGC
                     IIMLFVLDWRLMSVLVALIPITALLIYPIGRRMNRVAVEAQDAAADFSGELSKILYNIR
                     FIKATTSEKLEMKYTREILGRIFRLNIKEGILSAVIEPIVTIIIMLALVGMFWYGSAGI
                     AAGHITTGTLVSMIFYLFTMVSPLTQLSSFFSQYQKAKGASKEIYTIYNQQDEKQEKLG
                     NVPDMKSHDQNSDILHFANVSFSYNQGQPVLSNVELTFRGGTTTAIVGPSGSGKTTIFS
                     LIEKFYLEYNGEIYYNNVYLDKIRTEEWRRKISYVMQDSDLLVGSVRDNLVYGLNRDAD
                     DHEIYAALDTVGLKEVIYQLPDGLDTFVGERGVKLSGGQKQRIQIARCLLSDHEILLFD
                     EATANLDSESEQLVHDVIKLLSKNKMTIVIAHRLSTIYKADQIIFLDKGEVTGVGTHHE
                     LMSWHDKYRDYVQFQLPTQLS"
     gene            8231..8644
                     /gene="agrB"
     CDS             8231..8644
                     /codon_start=1
                     /gene="agrB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyclic-lactone-autoinducer: AgrB"
                     /gene_kind="biosynthetic"
                     /note="accessory gene regulator B"
                     /product="AgrB"
                     /protein_id="AFS60106.1"
                     /sec_met_domain="AgrB (E-value: 5.5e-23, bitscore: 70.8,
                     seeds: 181, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLLSYTVVRQKSFGWHARKSHHCTWVSLLTFVFVPWLLKEVEWNR
                     IFLATLCLLSIILIARYAPADTECNPLVDPKFRRACRLQAIFLSIVFVFVVIALDFYPW
                     LIFGLFLEAVVITPVFYSIMRRSYANYEQYQEY"
     gene            8613..8786
                     /gene="agrD"
     CDS             8613..8786
                     /codon_start=1
                     /gene="agrD"
                     /note="autoinducing peptide"
                     /product="AgrD"
                     /protein_id="AFS60107.1"
                     /transl_table=11
                     /translation="MPIMNNIKNIKEHRNNHTQRLKAKLLEHMSKRGEGGGICFGLFYE
                     PVNPKVWKLNKK"
     gene            8783..10111
                     /gene="agrA"
     CDS             8783..10111
                     /codon_start=1
                     /gene="agrA"
                     /product="histidine kinase"
                     /protein_id="AFS60108.1"
                     /transl_table=11
                     /translation="MITSGIYFLQYTSYLVIYHMVSGNGLSVFRMNYKLLLFLVMYYFI
                     GITALNNFGLIVYLFLFVLLALYGWLTDKYESLWVIGFYAILTIFITTLLGYFITVISY
                     AVPYEYKNKWTAPFLTACSPPLIYLALRKLVSYLFPTPAVRLLKLYRIKLIVGASLILV
                     LGIIGLGLIIYSECESNNPYPSRLKIVLMLIGLTSIFLMTINWLYRFLRERELEKNKTE
                     QYLQLEAYIMEIEKMYEDIRGFRHDYTNILLMLDEGIRTGDLAMIKRIYESSIQPTASQ
                     LNGYSYSLDNLINLDVSELKSIITSKVLVAKKRGVDVKLEIDSGISEMYMDIIDLCRVI
                     SCFLDNAIDAALETCNPSINIVLIHEDDIQTVTIRNSYNHSALDGIKVNDLYKRNFSTK
                     ADNRGLGLYNIAKVLRSNKYVTLDTKKEPDFFTQTLIIKRATS"
     gene            10153..10887
                     /gene="agrC"
     CDS             10153..10887
                     /codon_start=1
                     /gene="agrC"
                     /note="response regulator"
                     /product="AgrC"
                     /protein_id="AFS60109.1"
                     /transl_table=11
                     /translation="MEKMVRKSCEELGICCSNLIVTSKPDLLLFESKACANNLYFLDIE
                     ISGCQMKGLEVAQEIRKYDMYGHIVFVTTHGHLSPITFQYKVAALDFIIKDATQDEMEK
                     KVREVLVVAHQRKPVLDVNDWFILDNKFTKFQISFSSIYYFETTGVPHKIRLVSENRTL
                     EFYGDLKDIEAKDPRLFRCHRAFIVNLMKVESIDKSKKEIIFQTNQNSGEARRIIPVSR
                     QLLKHLLDKMGDRSLLNRPVYE"
     gene            10922..11692
                     /gene="paenN"
     CDS             10922..11692
                     /codon_start=1
                     /gene="paenN"
                     /note="putative acetylase"
                     /product="PaenN"
                     /protein_id="AFS60110.1"
                     /transl_table=11
                     /translation="MGRNMEQRLSVQAEVKGIDATSLKLIAVLAMLIDHIGGLFFADFL
                     GFQVVGRITLPIMAFFVAEGYIHTRSFKKYTMRLALTGLASVVPYGLVFKDWYTSGNIM
                     LTLLMGLLAIWFYDNTTNPVLKYLAISALCVVSFLGDWSVIGVFLVLAFYVARKQQKIY
                     NGWIIAIIALMFIFGVIIIWAVPDSLSIMGINASTMNEYLYPFAIGGACIFALPLLSRY
                     NGERGKGSRAFFYLFYPLHLLALYLLKLAIEYYI"
ORIGIN
        1 atatattctt tattcatcga cagcaagata ttacatacgg ttactcacaa tgaacgtgat
       61 ttaggggtac cactgaaatc atgtttatta gaatttaatt cagagaggaa gaggaaaatg
      121 aaagtagatc aaatgtttga ccttgattta agaaagagct atgaggccag tgagcttagc
      181 ccacaagctt caatcatcaa gacaaccatt aaggtatcca aagcagtatg taaaactctt
      241 acctgtatct gtaccggttc ttgttccaat tgtaaataat tcataagcga ttcgtaagtc
      301 aggggtagtg aaatgggcta cccctactta cattatggag attgcatgat aaaacctaat
      361 atatacaatt ggcatgtagc ccggctaacc gatcatttta gttgtggtgc aactgttggt
      421 cagccgctta ctatcctgtt tattgataca ggcgtcgata aatcccactc agagatccaa
      481 ggtaatctag ttgaggcaga atgcaagtca tttgtacctt atgagccttg tataagcgat
      541 tacgtcgggc atggtacaca aatggttagc tctgttaccg gcaaatatat attgaagggg
      601 atatgccctt tcgctcgggt tgttgtctac aaaattacgg acaaaaaagg aataagcaag
      661 cttgaatggt tgtatagagc gctggaagag ggtattgcta aaggatatcc aattatcaac
      721 gtcagctatc gtacagaata tgaggtcgac ttattcgagt tcaataggtt tcaggctttg
      781 gttgactgcg ccaatgcgca aggcatatgg gtagtctgtt ccgcaggaaa cgatggacgc
      841 gatattacgc aggagtacag agtacctgcg aatttaaagg gagtataccc aattggatca
      901 actaataaga acggggatat ctccgggttc agcaatacgc tcaacgatca attttttgcg
      961 ccgagtggtg atgagtatga aatcaattac aacaatgatt gggtaatggt tgccaaatcg
     1021 agcttcgaca cagaggatta cttatacgaa gtgctgggat ttgacaaagc atacacagtg
     1081 ggtatcggta cgagtattgc cagcgcctta ttcagcggag ttgttgcctc tcttagcgga
     1141 gagcttatcg cgcaggggat gaatgccgga cgcacagaaa tggagtgtct gctccgtcaa
     1201 ggcgcgagac tgattgatgt tcagcggaat tattatgaag taagccttgc cggttcaatt
     1261 cgtgctttaa gacagatgag aggttgattg catgttggag aactttgaaa tctcggaaaa
     1321 ttacatgtac cggaaaacgt tgcttggcat ggagcattat acgcagatat ttgatcatga
     1381 acaactagat gatgatgaat accttgacag acttgtacgg ttcgttcagc agcaggggtt
     1441 gatagattcc atttatgtct ccagcccctc gacctacgct gcactgcaaa gcctggaaca
     1501 aggcagaaca ttgaagtcca aaaaaaagcg gaatcttatc ctaagtctaa gcaagttcat
     1561 ttcgcgggca atttgtagac caacaccgtt tggactgttc gcgggagtag gcatttcccg
     1621 atttggaaaa acgggaggcg ccggggaaca tgtcgtctgg gcaactccgg attatagcct
     1681 aatttaccgc ttaatagggc ttttccatgg ggatgcggat gtccgcaatg aattgagact
     1741 gagtgtaaat aacacctctt acattgacca tcacaggctc gtcatctctt atcagactct
     1801 gtttgcagca gatcaggagg aacgaagcac tgaaaatata tctattctca aaaccccggt
     1861 catagatttc atcatgaaga tccttgataa agagcagcag acttttctgc agcttatgca
     1921 agctataaaa gacgccttcg gcgtggatga cacgatcagc acagattttt tgcagcaatt
     1981 gatagaacaa ggctttttag taagtgagat gattcccaat cctcgggata ccgctcctct
     2041 ggagcatctg atacgtacac taaagctgct tgctgctccc aaggcagcat attactcaga
     2101 tattttggag agagttggac aagccattca ggacattaca gttgctgcgg tcaaacgcga
     2161 aggcattgag gctgtccgaa aactagtcca gcttatcctt cccgattatc agggcgactt
     2221 ggtcaaaata gacgtaagac tgaatcaggt aaatgaaatt ctattgtcgg acagcgatag
     2281 gcaatcgttg gcccaaatag cttcaatatt gagcatgctt tctacgtaca ggaaagagga
     2341 tacactttcc gactatcgtc agcaatttac tgagaagtac ggagtatatg aagaagtgcc
     2401 cttattgcag ttgttcagta agaccttggg gctcggcaaa cctgaagagt acaagagcca
     2461 ctaccagaac ggagataagt acaggcacaa agatgaaggc aggttccgac agcttaaggc
     2521 gctaattgcg gagtggcaga ccgaagcgct tgtcaatcat tcggatatcg tgcttgacga
     2581 ggtcaaaatc caacagctgt ctgaattgag tctggagaat gtccatgtct cgttcgattt
     2641 gtattattcc tgcatggaga atgagcaagg tgaagttacg ttttatttaa acaacagagc
     2701 cggatcattg gaagcgtgtc agagcttcgg aagatttgcc tacatgtttg acaaggatag
     2761 ccgtgatgaa atcttcaatt ttgtcaggaa cccgaaggat cagaaagcag gggagagcgg
     2821 cgctgaaatc tcattttacc cattcagtcc caaaataact aatattatga cggcaggccc
     2881 gacaccagac caatctatgg atatggccac gtttcccgag aatcggaaat tagatgtttc
     2941 ccagctgctg gtaggggtgg aggattcggg atacttttat ctgaagcaca gggaaacagg
     3001 cgaactggta tttcccaaac tcagcagcat gtacaataca gagctggcgc cttatctgat
     3061 ccggtttctg aatgatatta atctgcagta tcagaccgga tggtcccatc tggaggagta
     3121 tctctatgat tcttctttta caccgagagt aacctataaa aatatcatca tctgccccaa
     3181 aaaatggaca ttatatgcgg ataaacagct ccagagcgaa gaacagtttc tggcctttct
     3241 ggaggattgg atcataaagt ataacattcc gcgatatgta tatttggtgg agctggacaa
     3301 taagctgttg ctggatttgg atcgccatca tcatcgaaag cttttgcaca aagaatatac
     3361 ccggaatccg gaaggcaagg cggccgttat tatggaaacg gagaaagaat tgttcgcttc
     3421 cgctaaccga tatatggagg agtgcgtatt tttttgcaaa agcccgatgt ttcagtactt
     3481 gccgatagac agattgtctg cacaggctac cgataaaagc ttaaataaag tgctgagttc
     3541 ttcggtgaat gccgtttatt atccaggttc ggattggata tccctgcagg tatattacaa
     3601 tcaggaaatg ctaaccgatt tgctgggcag tgcatttaag acattcatgg acaatagcgc
     3661 cgagtacttc attgaccagg ttttttttgt ccagtatgca gataaggagc cgcatatcag
     3721 gctaaggtgc aaaatgtcgg gaagcgtaga gcatcagcgt ttcacggcct tgaactatgt
     3781 ttttgatttc ctgaatactg taacctcgtc cggattggtt agttcctatt cggttgttcc
     3841 ttaccggcct gaaattttgc ggtatggcgg cgcagccttc attgaacaag cggagaggtt
     3901 atttgctatc gattcttatc tagtttcagg ctattatgca ggcaagaggt caattaatgc
     3961 aaccgatcaa atgttatttt gctgtaccgg actttttgag gttgtgaact gcgcgtatcc
     4021 gctggcagaa gatcaattga aggctatggc tgcgattatt gatccgaaga aattcagaca
     4081 ggagtatcgg gaacacaaag aagcgctatt tcaaaggatt caacagaccc gttccatatc
     4141 cttgccagca agcgatacag gcgacaggtt gtggcaatac gctgcatatt tcacggctat
     4201 ccgggaagct gacgacagaa ccaattatta cgatgatatt atgttcagca tcatgcacat
     4261 gttctgcaac agagtgttcg gcctggaccg ggaaaaggaa gagcttgccc tacacctgtg
     4321 ctactacagt ttggaagatt atttacaggt aagcaaatac catgttgctt attgaggagg
     4381 agcttatggc ggtcaaacaa gtcccgggaa atttatctga aatagtcagg caaagcgcag
     4441 aaatctacaa agatatagat actgtaaccc tgctaatcaa gaagcacgcc atgcatgatt
     4501 accctgaaat ggagctgata tcattggctt caggatatcc cgggatcagc cttcttctgg
     4561 ataaactaga tgaagtctac ccgggggaag ggtgggatgc gcttgctttc aagtacaatc
     4621 aggagattgt gcgcttaatc ggcaatatac cgttccattc attgtcactg tataccggat
     4681 tggcaggtgt tatttatctg ttcaacgagt gttcaagagg ttcaacaaga tatacgaatg
     4741 tgctctcaca gacgctggat atgtttctta gcagctatgg ggaattctat gaggagacaa
     4801 gggggaaatt ggaagactcc ttgattgagg atagcgactt ggatttgatt tcaggatgga
     4861 cggggattgc agctgcatta atggaggtta aggaacgcca agacagatac gctgggaagc
     4921 tggatgcggt gctccttaag atccttcaac tgattacgga tgctgtaggc aagctgagac
     4981 atttcgggga acttcccgaa cagaccattt actataatct tggtatggcg cacggaatta
     5041 caggagccat taatttgctg gctgttgcac atctccgccg ctacccgtta acaggattga
     5101 gagaagtact ggaagttgcc aagacttttc tactcggaaa tatcagagag tggaacggca
     5161 ccattgtcat acctaatatg ttcacaccgg atgaatccgc agagactagt cggaatccag
     5221 gttatcacag agatgcatgg tgttacggca caccgggagc ttctgtggct atgttccggc
     5281 tgggattggc cctggaggac aaagcgctgc agcagcaggc tctgcaattg ttcaataccg
     5341 tctactccag accagattcg ctaagaaagg taatttcacc gacgatctgt cacggctatg
     5401 caggcttgct cctgattcag caccatttca gccgtacggc agccataccg ttcaagaatg
     5461 attttttgca tcgtatgtta tcataccgga atactcaaga agcagtaccc tttacagacc
     5521 ttgagggaag cgtcgatgat ccgttatctc tcgataaagt aggcctgctc gatggatgtg
     5581 caggtgtatt gctgacactg ctgacctgtg ataatgagaa cttgagtaca tggaagcaga
     5641 tgctggtatt tagctaaaag gaggagacgg taatgttcat tttattaaaa gaaattatta
     5701 cagtcatggt gggactgatt accgattttc tctcttttca actggatgca tatttttata
     5761 tttggtttct ggtcctgttt atttcaagct tggcggcgac cctggttcaa cgaagcgaag
     5821 ggactattac ctacagcaca gccgaatgtg tcggaataaa tgtgctagtt gtcgtattat
     5881 acctagtaat cggtacaatt ttggccagat actttgcaag ttttgaggat cagcagattg
     5941 ttgctggaga taagattatg ctgtataaaa tttcttttat gcttacatcc gctgtaatta
     6001 tagtggatat tatactagat aggttgaaga gagggcggaa attctcctac tctttggagt
     6061 ttcttgccgg aagcgccgtg tgcttcttac tgttccaatt aagcaaccaa acggactgga
     6121 taggaaccaa gctgtcgctc tcctatattg tcatggtagc gatagccgca ggtgtctata
     6181 caatggcaat gaagtttttg ctagaatacg aaaatcacaa agagaaatag gtgattaagc
     6241 cataatggaa ttgaatatgg agagcacggc atataaaaag atcggtgaag aaaaacaaac
     6301 ggcccattcc ttgctggagt taatcaaaat tgcgagatgg cccaagcttg cacttgcttg
     6361 gagcatcgta tttacactga tttccgccgc tttttcatta gtattgccga tcattacagg
     6421 caagcttgtc gataagatct cagctatgga gctgacgatc cagaccgcag ctgtgcttat
     6481 ttcgctgttt ctggccggag ggattttaaa cgccgtgtct tctctgctcc tcagatatgt
     6541 ggggagcaag ttttttcagg aactgcgact gaaaatttgg gaaaaaatta tccatttgcc
     6601 ggttaattat tttgataaca acgagaacgg caaaatcatc agcagaatga cgaacgacct
     6661 tgctattgta agtaattttc tggctatcac tctgccttct attctggctg atgtcctgat
     6721 ggtcactggt tgcatcatca tgttatttgt gttggattgg agattaatga gtgtactcgt
     6781 tgctctgatt cctataacgg cgctactaat ctatcccatt ggaagaagga tgaatagagt
     6841 agcggttgag gcacaggatg cagccgcaga tttttccggt gagttatcca aaattctcta
     6901 taacatcaga ttcattaaag cgacgacgtc ggaaaagctt gaaatgaaat atactcggga
     6961 gatacttgga cgtatctttc gcttgaacat caaagaggga atattgtcag ctgtcattga
     7021 gccgattgtc acaattatca tcatgctagc tttggtagga atgttttggt atggaagcgc
     7081 cggcattgcc gctggacata tcactacagg tactttggta tccatgatct tttatttgtt
     7141 tacaatggtt tcgcctctga cccagctcag cagttttttc tcgcagtatc agaaggccaa
     7201 gggagcaagt aaggaaattt ataccatata taaccagcag gatgagaagc aagaaaagtt
     7261 aggcaatgtg ccggacatga aaagtcatga tcagaattcg gatatcctgc attttgccaa
     7321 tgtttctttc tcgtacaacc agggccaacc ggtattatcc aacgtggagt taaccttcag
     7381 aggcggtacc actacagcga tcgtagggcc ttccggctcg ggaaaaacaa caatattttc
     7441 cttaatagag aaattttact tagaatataa tggagaaatc tattacaata atgtatatct
     7501 ggataagatt aggacggaag aatggcgtcg caaaatatcc tatgttatgc aggatagtga
     7561 cttactggta ggatcagtga gagacaatct ggtatatggc ctaaacagag acgcggatga
     7621 ccatgagatt tatgcggctc ttgatacagt aggcttgaag gaggtaattt atcaattacc
     7681 ggatggtttg gatacctttg ttggagaaag gggagtcaaa ttatcggggg gacagaagca
     7741 gcgaatccag attgccagat gcttgctttc agaccatgag attctgctgt ttgatgaagc
     7801 taccgccaat ctggatagcg agtcagagca gcttgtacat gatgtaatca agcttctctc
     7861 caagaataaa atgaccattg ttatcgctca tcgtttgtca accatctaca aagcggacca
     7921 gataatcttt ttggataaag gagaagtaac gggggtaggc acgcatcacg aattaatgag
     7981 ttggcatgat aaatatcgtg attatgttca attccagctt cccacacaat taagttaggg
     8041 aggggtggtg gcgttgtatc atcaattgtc aacgtggatc gttgataaat actttcaaga
     8101 cgaagaattg gaagcggaga agcgtgctta tgtgctttat tatctggaga acatactggc
     8161 tggcacagaa aaaatacttc ttgtattggt cagtgcctgt ttgctcggtg tattagccga
     8221 aacggctgcg atgctgcttt cctatacagt ggtccggcaa aagtcctttg gctggcatgc
     8281 tcgcaagtcg catcattgca cctgggtcag tctactgacc tttgtgtttg ttccctggct
     8341 cttaaaggaa gtagaatgga accgcatttt tctggcgacg ctctgtctgc tttctatcat
     8401 tctgattgcc agatacgctc cggccgatac ggagtgcaat ccattggtag atccgaagtt
     8461 cagaagagca tgcagattac aagctatctt tttaagtata gtgtttgttt tcgtggtcat
     8521 cgcactagat ttttatcctt ggttaatatt tgggctgttt cttgaagcag tcgtcatcac
     8581 accagtattc tattccataa tgagaaggag ttatgccaat tatgaacaat atcaagaata
     8641 ttaaagaaca cagaaacaac catacccaga gactcaaggc taagctactg gagcatatga
     8701 gtaaaagagg tgaaggagga gggatttgtt tcgggttgtt ctatgaacca gtcaacccaa
     8761 aagtttggaa attgaacaaa aaatgattac atccggcatt tattttttgc aatacaccag
     8821 ctatcttgta atatatcata tggtcagcgg caatggtcta agcgttttcc gtatgaatta
     8881 taagctgctg ctgtttttag tcatgtatta ttttatcgga ataacagctc tgaataactt
     8941 cggactgatt gtttacctat ttctatttgt gctactggca ttatacgggt ggttgaccga
     9001 caaatatgaa tccttgtggg tcataggctt ctacgccatc cttaccatct ttattacgac
     9061 actgctgggt tatttcataa cggtaatcag ctatgcggtt ccttatgaat ataaaaacaa
     9121 atggacggcg cccttcctaa ccgcttgttc acctccgctg atctacttag cgctccgaaa
     9181 gcttgtgtct tatttgtttc ctactccggc ggtcaggctt ttgaagctat atcggattaa
     9241 gcttatcgtc ggagcttctt taatcttagt cttggggatc atcggactag gactcattat
     9301 ctattcggag tgtgaaagca acaatccgta tccaagcaga ttaaagattg tgttgatgct
     9361 gatcggcttg acctccatct tcctgatgac gatcaattgg ctgtatcgct tccttcgtga
     9421 acgtgagcta gagaagaaca agacggaaca atacctgcag ctggaggctt atattatgga
     9481 gattgaaaaa atgtacgaag acatccgcgg cttcagacat gactatacca atatcctgtt
     9541 aatgctggac gaggggatca gaactgggga tttggcgatg atcaagcgga tatatgagag
     9601 ttctatccaa cctactgctt cacagttgaa cggatattcc tatagtctgg ataacctgat
     9661 taatctggac gtgagtgagc tcaagagtat tattacatcc aaggtgctgg tggcaaagaa
     9721 gcgtggagtt gacgtaaaac tggaaattga tagcggtatc agtgaaatgt atatggatat
     9781 tatagaccta tgcagagtaa tctcttgttt cctggacaat gcaatcgatg cggcactaga
     9841 gacctgcaat ccgtccataa atattgtact gatccatgaa gacgatattc agacggtgac
     9901 gatacgaaac tcttataatc attctgcact tgacgggatt aaggtgaatg atctctacaa
     9961 acggaacttt tcaacgaaag cggacaatcg gggtcttggc ttgtacaata tagcaaaggt
    10021 gctgcgcagc aacaagtatg tcactttgga taccaaaaag gaaccggatt ttttcacgca
    10081 aacattaatt ataaagagag cgacatcatg aatattttta tcctggaaga tcagtttatc
    10141 cagcagcgca taatggaaaa gatggtccgt aaaagctgcg aggaacttgg catctgctgc
    10201 agcaacctca ttgtcaccag taagccggat ctcctgcttt ttgaatctaa agcttgtgcg
    10261 aataacttgt atttccttga tattgaaatc tctggttgtc agatgaaggg actggaagtt
    10321 gcccaggaaa tcagaaaata tgatatgtat gggcatattg tctttgtcac tacccacggt
    10381 catctgtctc cgattacctt ccaatataag gtagcagctc ttgatttcat tatcaaggat
    10441 gctactcagg atgaaatgga aaaaaaggta agagaagttc ttgttgttgc ccatcaaaga
    10501 aagcctgtgt tggatgtcaa tgattggttt atactcgaca ataaatttac gaaatttcaa
    10561 atttccttct ccagcattta ttattttgaa acaacgggtg ttccccacaa aatccgtctg
    10621 gtttccgaaa acagaactct tgagttttac ggcgatctga aagatattga ggctaaggac
    10681 ccaaggttgt tccggtgtca tcgggcattt atcgtaaacc tgatgaaggt ggaatctata
    10741 gataagagca agaaggagat catttttcaa accaatcaga attccggaga agcaaggcgc
    10801 attattcccg tttcccgcca attgttgaag cacctcttag ataagatggg agaccggagc
    10861 ctcctgaaca gacctgttta cgaatagaat agacacggag ggcatagtcc ttcgggaaga
    10921 aatggggagg aatatggaac aaagattatc cgttcaagca gaggtgaaag gcattgatgc
    10981 caccagttta aaattaattg cagtattggc aatgctaatt gatcatattg gagggctatt
    11041 ttttgccgat tttttgggct ttcaggtggt aggcagaatt acattgccta tcatggcttt
    11101 ttttgtagcc gaaggatata tccataccag aagcttcaaa aaatatacaa tgcgccttgc
    11161 tctaaccggg ctagccagtg tggtgcctta cggattggta tttaaagatt ggtatacttc
    11221 gggaaacatc atgctaacgt tgttgatggg cctactggca atatggttct atgacaacac
    11281 gaccaatcct gtacttaaat acttggcgat ttcagctctt tgcgtagtct cttttttggg
    11341 agactggagt gtaattggag tgttcctggt tctggctttc tatgtggctc gcaaacaaca
    11401 gaagatttat aacggatgga ttattgcaat aattgcgctt atgttcattt tcggagtcat
    11461 cattatatgg gctgttcccg attcgctcag catcatgggc atcaacgcca gcacaatgaa
    11521 cgaatatctt tatccgtttg cgatcggcgg ggcctgtatt tttgccctcc ctctgctatc
    11581 ccgatataac ggagagaggg gaaaaggctc acgagccttc ttctatttgt tttatcctct
    11641 gcatttgctc gcgctctatc tgctgaagtt ggctattgag tattacatat aa
//
