LOCUS       BGC0000541              9113 bp    DNA     linear   BCT 29-JAN-2013
DEFINITION  Paenibacillus polymyxa strain NRRL B-30509 lantibiotic paenicidin A
            gene cluster, complete sequence.
ACCESSION   BGC0000541
VERSION     BGC0000541
KEYWORDS    .
SOURCE      Paenibacillus polymyxa
  ORGANISM  Paenibacillus polymyxa
            Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae;
            Paenibacillus.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 13:48:48
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     gene            1..177
                     /gene="paeA"
     CDS             1..177
                     /codon_start=1
                     /gene="paeA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR03731"
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /note="paenicidin A structural gene"
                     /product="PaeA"
                     /protein_id="AFW17838.1"
                     /sec_met_domain="TIGR03731 (E-value: 1.2e-10, bitscore:
                     30.3, seeds: 23, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAENLFDLDIQVNKSQGSVEPQVLSIVACSSGCGSGKTAASCVET
                     CGNRCFTNVGSLC"
     CDS_motif       1..66
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence: MAENLFDLDIQVNKSQGSVEPQ"
                     /locus_tag="paeA_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       67..177
                     /prepeptide="core"
                     /locus_tag="paeA_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class I"
                     /score="11.80"
                     /molecular_weight="3323.8"
                     /monoisotopic_mass="3321.5"
                     /core_sequence="VLSIVACSSGCGSGKTAASCVETCGNRCFTNVGSLC"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MAENLFDLDIQVNKSQGSVEPQ"
                     /leader_location="[0:66](+)"
                     /alternative_weights="3341.8"
                     /alternative_weights="3359.8"
                     /alternative_weights="3377.9"
     source          1..9113
                     /culture_collection="NRRL:B-30509"
                     /db_xref="taxon:1406"
                     /mol_type="genomic DNA"
                     /organism="Paenibacillus polymyxa"
                     /strain="NRRL B-30509"
     misc_feature    1..9113
                     /note="lantibiotic paenicidin A gene cluster"
     protocluster    1..9113
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[2678:9113]"
                     /cutoff="20000"
                     /detection_rule="((LANC_like and (Lant_dehydr_N or
                     Lant_dehydr_C)) and not (YcaO or TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      2679..9113
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="((LANC_like and (Lant_dehydr_N or
                     Lant_dehydr_C)) and not (YcaO or TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
     cand_cluster    1..9113
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((LANC_like and (Lant_dehydr_N or
                     Lant_dehydr_C)) and not (YcaO or TIGR03604))"
                     /kind="single"
                     /product="lanthipeptide-class-i"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..9113
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000541"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..9113
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lanthipeptide-class-i"
                     /region_number="1"
                     /rules="((LANC_like and (Lant_dehydr_N or Lant_dehydr_C))
                     and not (YcaO or TIGR03604))"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            346..1074
                     /gene="paeF"
     CDS             346..1074
                     /codon_start=1
                     /gene="paeF"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 187.5; E-value:
                     5.1e-57)"
                     /gene_kind="transport"
                     /note="ABC transporter ATP binding protein"
                     /product="PaeF"
                     /protein_id="AFW17839.1"
                     /transl_table=11
                     /translation="MTDFLLEVKQVSKTVGKRSLLNNISFELEKGKIYGFIGPNGAGKT
                     TMMRIMTGLMQPTSGSVKIASYNVQTDREKALLKVGAIIESPVFFDYMTGRQVLRNLSR
                     LHLGVASSDREKHIKELLERVGLENRGDDKVKTYSLGMKQRLGIAQSMLGNPELLLLDE
                     PSNGLDPMGMRELRDIILRLRDSDNYAFFVSSHLLDELQQMCDELIVIKEGTLIWKGAA
                     DQLIKDGQRLEDAFMGLMGL"
     gene            1071..1871
                     /gene="paeE"
     CDS             1071..1871
                     /codon_start=1
                     /gene="paeE"
                     /note="ABC transporter membrane-bound subunit"
                     /product="PaeE"
                     /protein_id="AFW17840.1"
                     /transl_table=11
                     /translation="MKTLVYSEFERLWRRKWFWLLVLCTPVLVYSTGIYIQHTLRIPVK
                     DLSKIFIVAGLRENLYLICNFAVATLVAAVFTEEFRSGQLRLLFVRRFTRGQIFWSKLL
                     VVNTSILLLLIVYAVSSQVIVVFWKFSHNINGTDIFGQIWSQVFEYYGWAFASLLAISS
                     LYSFIAMCSKSVTYAIGICMTYNLGVLLFDPLYMKLATLFSSIPLLKNLIPFLLIPYLQ
                     YKGLDQSLIWNWSVNGAIVIAVLFHLILFTGWAYRRFTTDDYLY"
     gene            1884..2651
                     /gene="paeG"
     CDS             1884..2651
                     /codon_start=1
                     /gene="paeG"
                     /note="ABC transporter membrane-bound subunit"
                     /product="PaeG"
                     /protein_id="AFW17841.1"
                     /transl_table=11
                     /translation="MSGIWLSELERIWNRKSTFLLYIAMLLIIFVNIWSLKTNGTMRFR
                     FGEGTVVVNNLNLPWLMMSEISLFLILAMLPILYVDQLGGDLHSGAYRLYVLRPYRRFQ
                     FWLGKLLALSVTTAIFTGTALVIGIVAGWLLFPHTSTFFKYGSGVVSPSSDAIPYTLAF
                     YLILTLACVAKLLLVSAVCLFISRPLFAFIAIFVLSVALYQVVRPLVLLMDPFQQIMLA
                     LRVEGSFKFWMCLIAIILVGSVISFVRWQKKII"
     gene            2679..5855
                     /gene="paeB"
     CDS             2679..5855
                     /codon_start=1
                     /gene="paeB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lanthipeptide_LanB_RRE"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1155:Lantibiotic dehydratase domain protein (Score:
                     904.3; E-value: 1.4e-273)"
                     /gene_kind="biosynthetic"
                     /note="lantibiotic dehydratase"
                     /product="PaeB"
                     /protein_id="AFW17835.1"
                     /sec_met_domain="Lant_dehydr_N (E-value: 1.4e-179,
                     bitscore: 587.0, seeds: 73, tool: rule-based-clusters)"
                     /sec_met_domain="Lanthipeptide_LanB_RRE (E-value: 2.6e-15,
                     bitscore: 46.0, seeds: 20, tool: rule-based-clusters)"
                     /sec_met_domain="Lant_dehydr_C (E-value: 6.6e-38, bitscore:
                     119.8, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKTTGNLYKTLDFFMLRTPVLPIQEFSKCFPSSVKEGGDAREIAL
                     ERLLELSRDPFIREALAASSPTLLESLVHISNHENPRKQGQVIKAFMRYIIRMITRPTP
                     FGLLSGITYGRFGERSQLFLQGTDTFKKRARPDMEWLMKAVELVESRKEIVRQLRVRSN
                     KLIYRQGNRAKIPYTTRHGSMNDGEDQSVSVRITQVFDCVMAASSAFIPFLDLVDHLNK
                     TFESANIEVIERYVWQLFEQEFLISELIPPTTTTEPLSYIRSVLKPVTGIEELKEDLDT
                     IADTSAKYNHLPIGQGEQLLESLRQKMNKMFSAKSSLQIDLSLEDRGIILPQSIRKDIE
                     CVAELLTKLSTRENRPLNEFCVEFLEKYGPYREVPLLDLLDEDTGLGAPAGYKHPMSRR
                     VRLQDMSPSKSRSHQEKLLFQWFVSAIHEGKHEIVLTDNMIDELIKGHDTEKWLAAPSM
                     ELFFQLITSDQEALDQGEYTLLLAPNQGSDAAGKTFGRFLDLFGEPFNDIFSSIQSEEQ
                     HLQPGKLLAGISYLPSSGRSANVVLTKQTRPYEIAVGANHTMPEDRQIHISDLVVGVRN
                     DHFYLKSKSRNREVIASSGHMLNAELTPNIYRFLVEVSDNSYRHWSPMEWGFVAHAPFT
                     PRLRYKHIILYPGTWRIASSSSKKMADFAEEIRLFQEKWNIPRYVYMIESDNRILLDMD
                     HPLHIEQICRDLQSKGKVTLIEHIGGLDNSPVSCEKGHLTAEFVCPLVRNQAEDTQHFV
                     EETAASLELVAKENEQQPTKADYTYLPGESWFYAKLYGINSRQNEFIGGYWEEFVRKHQ
                     DNGTILQAYFIRYADPDKHLRVRFELSKNRTADGFLSTFHKWTEMLLHEGMIARVVIDT
                     YEPEIERYGGSKPMAWAERMFSCDSEVTAKLVHLIRFNQVSLEEDIIATLSVINLLYQF
                     GYHEKDAFNLLNRYFDVKEYLENFRKERKFFLQWFASDQEISQSIQSNLQAIESIFKIR
                     REAVKQYSHVLYEHETGGLSTNTREDIVFSVIHMHLNRLLGIDRDRERKIMIMARHALN
                     SLVQYRGNRQ"
     gene            5852..7729
                     /gene="paeT"
     CDS             5852..7729
                     /codon_start=1
                     /gene="paeT"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 186.5; E-value:
                     1e-56)"
                     /gene_kind="transport"
                     /note="lantibiotic ABC transporter"
                     /product="PaeT"
                     /protein_id="AFW17836.1"
                     /transl_table=11
                     /translation="MTDIQAKMNLESSTIWRSVRQGVKLFSLVFGMNKTYMVSSIFLHI
                     LQGLMPVLTLIIMQNLLNSIVSFGTQGISLIMLQFGIFIVIHVLKSVVTMSQTFVEGTL
                     ESVLSKSLNLMIGKKSSSLGLADYENSEINDQLKRASQEASYRPYQLYTQMAGILSSMV
                     TMVSSATLILLWKWWVVIILFGISLVSVYSIFKLNKEQFEIYMNRTPLYRESWYMTYLL
                     TNDRAVKEVKIFNMSSYLLKRYEDLLQRFFVEDRRILGKRVTITFMYSLLELAVLLGVV
                     WVAIRETFEKKILVGSLYGYIQAIMLTQSQMQALIQGIVQFSQNNLYMEQLFLFLQLPT
                     SDPVRQQKEQALPINRKKNEDFCIEQICIEGVSFTYSGKTVPSIRDIHLTVNRGQTIAI
                     VGKNGSGKSTLMKVLMQLYSNYEGVFKINGLPVQDYDLHQVQDKIGVVFQDFMHYEMSA
                     RHNIGFGSLERLEKDEELIQAARYAAIDQIITELPQGLDTQLGKWFEDGFQLSGGQWQR
                     IAIARAFLRQADIYILDEPSSFLDPVAERDLLKLFLELMKDSIGIFITHRISSARLADK
                     IVVMDNGGIVEQGTHRELMLLDGLYAEMYNIQASSFNDEEALAETGGRV"
     gene            7731..9113
                     /gene="paeC"
     CDS             7731..9113
                     /codon_start=1
                     /gene="paeC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: LANC_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1140:Lanthionine synthetase C family protein (Score:
                     317.7; E-value: 1.8e-96)"
                     /gene_kind="biosynthetic"
                     /note="lantibiotic cyclase"
                     /product="PaeC"
                     /protein_id="AFW17837.2"
                     /sec_met_domain="LANC_like (E-value: 1.5e-58, bitscore:
                     187.1, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTISYTCDNWIPVKGEMKDKISTLLREITERYRNPDQVRLWMKK
                     IPPIEADGVIMERWADESLGSGFTGICLLMGQLDYLYPKNGWDIVGHQYLERIRMVVEQ
                     KGVHDLSLYSGLAGIMLAIYSLSRKGTRYQGMLDALASWFEEAALQSLALHRIEWEKGE
                     LRMSQYDTISGFAGVGRIAMLFLNRPGMQMVWEQTMDLFHVMCGEKNLNGHTVPAWHIS
                     SEHQFQEDEKLQYPQGNFNLGISHGIAGPLCFLSLSALHGVDASPLMNDIHRLGKFIHH
                     WRVQDVEGIIWPGRISFGEWLDGQLLSGSTRNYRDSWCYGVPGIARSIWLAGQAVHNEE
                     WLETGLSAYLDIEKRLHRKGGLSSATLCHGIGGLLHLVQRMYSDTSHEQLGRIRDRLLE
                     DVLDMFDANSLFGFYDHSLLDGRLAEVDEAGFLNGSAGVALVLASLIGDQNPDWDAAFL
                     IR"
ORIGIN
        1 atggctgaaa acttatttga tctggacatt caagtaaaca aatctcaagg ttctgtagag
       61 cctcaggttc tgagcattgt tgcatgttct agcggatgtg gtagcggtaa aacagctgcc
      121 agttgtgttg aaacttgtgg caaccggtgc tttactaacg ttggttcact ctgctaatct
      181 cgattgttgc ttttttagat aatggatttt attgtactcc ataaaagaaa aacaagaagg
      241 agggacctta aagtcccttc ttcttgtttg ataaatattt attttttaac tatataatat
      301 ttagagatat ttttgatatc gtgtttggtg atggagggaa aaggtatgac cgatttctta
      361 ttggaagtaa agcaggtaag caaaactgta ggtaaacgaa gtttgttaaa taacatttca
      421 tttgagctcg aaaaagggaa aatatatgga ttcattggcc cgaatggagc tggaaaaacg
      481 acgatgatgc gaatcatgac gggactgatg cagcctacta gtggttctgt aaagatagct
      541 tcatataatg tacagacaga tcgggaaaaa gcgttgttaa aagtaggcgc tatcattgag
      601 tctccggtct tttttgatta tatgacaggc agacaggtgc tgcgtaattt gtcccgattg
      661 cacttaggtg ttgcatcttc ggatcgagaa aaacatatta aagaattatt ggaacgggtt
      721 ggtctggaaa atcgcgggga tgataaggtc aaaacctact cgctagggat gaagcaacgg
      781 cttggtattg cccaatctat gttgggtaac cccgagctat tgttgcttga tgagccttcc
      841 aatggtctcg atcccatggg gatgcgtgag ttgagggata taattcttcg tttgcgtgat
      901 tcagataatt atgctttttt tgtctccagc catctgttgg atgaattaca gcaaatgtgt
      961 gatgagttga ttgtcattaa ggaaggaacg cttatttgga agggggccgc tgatcaactt
     1021 atcaaagatg gccaacggtt agaggatgcc tttatggggc tgatggggct atgaaaacac
     1081 ttgtttatag tgaatttgag agattatgga gaaggaaatg gttttggtta ttggttttat
     1141 gcacccctgt acttgtatat tcgacaggga tatacataca gcatacgtta aggattcctg
     1201 tgaaggactt atccaaaatt tttattgttg caggcttgcg tgaaaatcta tatttaatat
     1261 gtaattttgc tgtggctacc ctggtggctg ctgtttttac ggaggagttt aggagcggac
     1321 agctgaggtt gttatttgtg cgtcgtttta cccgtggtca gatcttttgg agtaaattat
     1381 tggtagttaa tacttctatt ttacttcttc tgattgtata cgccgtatcc tcgcaagtaa
     1441 ttgttgtttt ttggaagttc tcgcataata taaacggtac agatattttc gggcaaatat
     1501 ggtcccaagt gtttgaatat tatgggtggg catttgcttc tttattagct atcagttcac
     1561 tctattcttt tattgctatg tgcagtaaaa gtgtaacata tgcaattggt atttgtatga
     1621 cctataatct gggtgttttg ttatttgatc cgttgtacat gaaattggct actttattct
     1681 cttctattcc actcttaaag aatctgattc catttttatt aattccatat ttacaataca
     1741 agggactaga tcaatcactg atatggaatt ggtccgtcaa tggggcaatt gtgattgccg
     1801 tcttgttcca tctgattctt tttacagggt gggcttaccg ccgctttaca acggatgatt
     1861 atttgtatta ggaggatcta tttatgtctg gaatttggtt gagcgaatta gagagaattt
     1921 ggaatcggaa atctactttt ttattatata tagcaatgct ccttatcatt tttgtgaata
     1981 tatggagtct taaaacaaat ggtacaatgc gatttcgatt tggtgaggga actgtagttg
     2041 ttaataattt aaatctgcca tggcttatga tgagcgaaat ttctcttttt ttaatcttag
     2101 ccatgttgcc tatactgtat gtcgatcaat taggcggcga tcttcatagc ggagcatatc
     2161 ggctatatgt attgcgacca tatcgtcgtt ttcaattctg gcttggcaag ctgctcgctt
     2221 tatcggttac aaccgccata tttacaggta cggctttggt gataggcata gtggctggat
     2281 ggttactgtt tccccatacc tctacctttt ttaaatatgg ttcaggagta gtatctccct
     2341 catcggatgc aatcccatat acgcttgcat tctatttgat tttaacactg gcctgcgtgg
     2401 caaagttgct gttagttagt gctgtctgct tgtttatttc aagacctttg tttgctttta
     2461 tcgcaatttt tgttttatct gtcgctcttt atcaggtggt tagaccgctt gtcttactca
     2521 tggatccctt tcaacaaatt atgcttgctt taagggtgga aggttccttt aagttttgga
     2581 tgtgcttaat cgcaattatt ttggtcggct cagttatcag ctttgtacgt tggcaaaaga
     2641 aaataattta aaagtttgtt ttagaacgga ggttccttat gaagacaaca ggaaatctgt
     2701 acaaaacgtt agattttttc atgcttcgta ctcctgtatt acccattcaa gagttttcta
     2761 aatgctttcc gagcagtgtt aaagaggggg gggacgctag agagattgca ttagagcgtt
     2821 tattggaatt gtcccgtgat ccgttcattc gtgaagctct agccgcctcc agtcccactt
     2881 tgcttgaatc tttggtacat atcagtaatc atgagaatcc gcgcaaacaa ggacaagtaa
     2941 tcaaagcttt tatgcgttat atcattagaa tgattacacg gccaacgccg tttggcttac
     3001 tatctggtat cacatatggg cgatttgggg aacgatcgca gctatttttg caaggaacag
     3061 acacgtttaa aaagcgtgcg agacctgata tggaatggct aatgaaagcg gttgagctgg
     3121 ttgaaagtcg aaaggagatt gtgcgccaac tgcgggttcg gagtaataaa ttaatttatc
     3181 gccagggtaa ccgtgccaaa attccttata cgacaagaca tggcagtatg aacgatggcg
     3241 aagatcagag tgtgtctgtt cgtataactc aagtatttga ctgtgtaatg gctgcgagtt
     3301 cagccttcat accatttttg gatttggtcg atcatcttaa taaaactttt gagagtgcta
     3361 atattgaggt aattgaacgt tacgtgtggc aacttttcga acaagagttt ctgatttctg
     3421 aacttatccc acctacgacg actactgaac cgttaagtta tatacggtct gttctgaaac
     3481 cagtaacagg tatcgaagag ttaaaagaag atcttgatac aatcgcagat acttctgcta
     3541 aatataatca tcttcctata ggtcaaggag aacaactgtt agagagctta agacaaaaaa
     3601 tgaacaaaat gtttagtgca aaatcaagtt tgcaaatcga tctctccctg gaagatagag
     3661 gtatcatcct tccacaatct attcgtaagg atattgagtg tgttgccgaa ttattgacca
     3721 aactttcaac gcgtgaaaac agacctttga atgagttttg tgtggaattt ttggagaaat
     3781 acggaccata tcgggaggta cctttgctgg atctgcttga tgaggacaca gggctagggg
     3841 ccccggcagg ctataaacac cctatgagtc gtcgagtacg gttacaggac atgtctccat
     3901 ccaagtcgag gtcacatcaa gaaaagcttt tgtttcaatg gtttgtatct gctattcatg
     3961 aaggaaagca tgaaattgta ttgacagata acatgatcga tgaattgata aagggtcacg
     4021 atacggagaa atggctagct gcaccctcca tggaattatt ttttcagcta attacttctg
     4081 atcaggaggc tttggatcaa ggtgaatata cgttgctctt agcacctaat caggggtctg
     4141 atgcagcagg aaagacattc ggtaggtttt tggacctgtt cggggagcct tttaacgata
     4201 tattttctag tatccagtcg gaagagcagc atttgcaacc cggaaagctg cttgctggga
     4261 tatcttactt gcctagctca ggaagatcag ctaatgtcgt gttgacgaaa caaactcgac
     4321 cttatgagat cgcagtaggg gctaatcata caatgccgga agatcgacaa attcatattt
     4381 ccgatctagt cgttggtgtg cggaacgatc acttttattt gaaatcaaaa agccgcaatc
     4441 gagaggttat tgctagttcg ggacacatgc ttaatgcaga gttgacgcct aatatatacc
     4501 gttttctcgt ggaggttagc gataatagct accgccattg gtcgcctatg gagtggggat
     4561 ttgtggctca cgcccctttt accccacgtc tcaggtacaa gcatatcatt ctctacccgg
     4621 gaacatggag aattgcaagt agctcatcga agaagatggc agacttcgcc gaagagatac
     4681 gtttatttca agagaagtgg aacataccac gttatgttta tatgattgaa tcggataatc
     4741 gtattttact agatatggat catccgttgc atatcgaaca aatatgcagg gaccttcagt
     4801 ctaaaggtaa ggttaccttg atagagcaca ttggcggcct tgacaattca ccagtctcct
     4861 gtgaaaaagg acatttaact gctgagtttg tatgtcctct cgttcgaaat caagctgaag
     4921 atacgcaaca ttttgtggaa gagacagcag cttctcttga attggttgca aaagaaaatg
     4981 agcaacaacc aacaaaggca gactatactt acctgccggg agaatcttgg ttctacgcga
     5041 agctttacgg tataaatagc cgacaaaatg agtttatagg tggatattgg gaagaatttg
     5101 tgcggaagca tcaagacaac ggtactatcc ttcaggcata ttttatccga tatgccgatc
     5161 cggacaagca tctacgtgtc cgatttgagc tttcaaagaa taggacagca gatggatttc
     5221 tctctacgtt tcataaatgg acagaaatgc tgctgcatga agggatgata gccagggtgg
     5281 tcatcgatac atatgagcct gagatagagc gatatggtgg aagtaaaccc atggcctggg
     5341 cggagagaat gttttcgtgc gatagtgagg taacagctaa gcttgtacac ctcatccggt
     5401 ttaaccaagt ttccttagaa gaagatatta tagccacctt aagtgttatc aatcttctct
     5461 atcaatttgg ttaccatgag aaagatgcgt tcaacttact gaatcgatat tttgatgtta
     5521 aggaatatct ggagaatttc cgcaaagaac ggaagttttt tttacaatgg tttgcatccg
     5581 atcaggaaat ttcacaatcc attcaatcca atctccaagc tatagagagt attttcaaga
     5641 taagaagaga agccgtaaag cagtatagcc atgtactcta tgagcatgaa acggggggat
     5701 tatccactaa cacaagagaa gatatcgtat ttagtgtaat tcatatgcat ctgaatcgac
     5761 tgctagggat agacagggat cgagaacgga agatcatgat tatggccaga catgctctga
     5821 acagtttagt tcaatatcgg gggaatcgac aatgaccgat attcaagcga aaatgaatct
     5881 agagtcttcc actatctgga ggtcggtgcg acagggggta aagttgtttt cgttggtatt
     5941 tggtatgaat aagacgtaca tggtatcaag cattttcttg catattcttc agggcttaat
     6001 gccagtcctg acactaataa ttatgcaaaa ccttttaaac agcattgttt ccttcggcac
     6061 tcaaggcatc tccttgatta tgttacagtt tggcatattt atagtgattc acgtactgaa
     6121 aagtgtagtt acaatgagcc aaacgtttgt tgaaggtacg ctggaatcag tgctgtctaa
     6181 gtctctgaac ttaatgatcg ggaaaaaatc aagctctttg ggattagctg attatgagaa
     6241 ttcagaaata aatgaccaat taaagcgggc ctctcaggag gcctcttatc gaccgtatca
     6301 actgtataca caaatggcag gaattttatc cagtatggtt accatggtct cttccgcaac
     6361 tttgattctt ctgtggaaat ggtgggtagt tatcattctg tttgggatct ctctggtatc
     6421 tgtatactct atttttaagc taaacaaaga acaatttgaa atctatatga atcgaactcc
     6481 attatatcga gagtcttggt atatgaccta cttgttgacc aatgatcgtg cagtcaagga
     6541 ggtcaaaatt tttaatatga gttcttattt gcttaagaga tacgaggatt tgctacaaag
     6601 attttttgta gaggaccgac gaattctggg gaaaagggtc acaatcactt ttatgtacag
     6661 tctgctggag cttgctgtgt tgttaggagt agtatgggtg gctattaggg agacctttga
     6721 gaaaaaaata ctagtaggta gcttatatgg ctatattcaa gcaattatgc tgacgcaaag
     6781 ccagatgcag gcattgattc agggaattgt tcaattctcg caaaataatt tgtatatgga
     6841 acagttgttt ttatttttac agcttcccac atcagatccg gtccgacagc aaaaggaaca
     6901 agcgctaccg attaaccgaa aaaaaaatga agatttttgt attgagcaaa tatgtataga
     6961 aggtgtttct tttacttatt cgggtaaaac agtcccctct atacgtgata ttcacttaac
     7021 cgtgaataga ggccaaacta tagctattgt aggaaagaac ggttcaggta aatcaacgct
     7081 aatgaaagta ctgatgcagc tttactccaa ttatgaaggt gtgtttaaga ttaacggtct
     7141 tcccgttcag gattatgatc tgcatcaagt tcaggacaaa atcggagttg tgttccagga
     7201 ctttatgcat tatgaaatgt cagcacgtca taatatcggc tttgggtcgc tagaacgttt
     7261 ggaaaaggat gaagagctca tccaagcggc tcgatatgca gctatagatc agattataac
     7321 cgagcttccg caaggattag acacacaact cggaaaatgg ttcgaggatg gcttccagct
     7381 ctcgggagga caatggcaga gaatcgcaat tgctcgggct tttttgcgcc aggcagatat
     7441 atatatttta gatgagccga gttccttctt ggatccagta gcagaaaggg atttgctgaa
     7501 gttatttcta gagttaatga aggatagcat cggaatcttt ataacacatc gaatttcttc
     7561 ggcacgcctg gcggataaaa ttgtggtcat ggataatggg ggaattgtcg agcagggaac
     7621 ccatagggaa ctgatgctac tggatggatt atatgcagag atgtacaaca ttcaagctag
     7681 ttcattcaat gacgaggaag ctttggccga aaccggaggg agagtatagg atgacgacga
     7741 tttcatatac atgcgataac tggatacccg tcaaggggga aatgaaggac aagatatcta
     7801 cattattgcg ggagattacc gaacgttata gaaacccgga tcaggtgcgt ttatggatga
     7861 aaaagatacc tccaatagaa gccgacgggg tgataatgga acgttgggcg gatgaaagct
     7921 tgggtagtgg gtttacggga atatgtttgc tcatgggtca gctggattat ctttatccca
     7981 aaaatggttg ggatattgta ggacatcaat atttagagcg aatccgtatg gtggtagagc
     8041 aaaaaggtgt tcacgattta tccttgtata gtggattggc aggtattatg ctggccattt
     8101 actctttatc taggaagggt acacgttatc aaggtatgct agatgcactt gcttcatggt
     8161 ttgaggaagc tgctttacaa agtctggctc tacatcggat tgagtgggaa aagggagagc
     8221 tgcggatgag tcaatatgac accatctctg gctttgcggg agttgggcgt atagctatgc
     8281 tgttcctgaa ccgcccgggc atgcagatgg tatgggaaca gacaatggat ttgttccacg
     8341 tcatgtgcgg tgagaaaaat ctaaatggtc acactgtacc cgcttggcac atttcctcgg
     8401 aacatcagtt tcaggaggat gaaaagctac agtatcctca ggggaatttt aatttaggta
     8461 tttcgcatgg tattgcaggt cccttatgtt ttctgagctt gtctgccctt catggagtgg
     8521 acgcctcccc tttaatgaac gatatacata gactgggaaa gtttatacat cattggcgtg
     8581 ttcaggacgt agagggcata atttggcctg gtcgaatttc ctttggggag tggctggatg
     8641 gccagctttt atcaggaagc actcgaaact atcgtgattc ttggtgctat ggtgtaccag
     8701 gtatcgcacg ttctatctgg ctagcaggac aagctgttca caatgaagaa tggctcgaaa
     8761 cggggctttc cgcttattta gatatagaga aacgcctcca tcgaaaaggg ggattatctt
     8821 ctgctacatt atgtcatggg attgggggat tgttgcatct agtccaacgt atgtactcag
     8881 atacttccca tgagcagtta gggaggatcc gggacagact acttgaagat gtattagata
     8941 tgttcgatgc taattcgttg tttggcttct atgatcatag cttattggat ggaaggttag
     9001 cggaagttga tgaggctggt tttttgaatg gaagtgctgg agttgctttg gtgttagcct
     9061 ccttaattgg agatcagaat ccggactggg atgctgcttt tctaattcgc taa
//
