LOCUS       BGC0000549             15313 bp    DNA     linear   BCT 10-JAN-2012
DEFINITION  Streptococcus salivarius strain 5M6c salivaricin D gene locus,
            complete sequence.
ACCESSION   BGC0000549
VERSION     BGC0000549
KEYWORDS    .
SOURCE      Streptococcus salivarius
  ORGANISM  Streptococcus salivarius
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
COMMENT     ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2022-11-07 19:50:07
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 22602
            Orig. end    :: 37915
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     subregion       1..15313
                     /aStool="mibig"
                     /contig_edge="False"
                     /label="RiPP"
                     /subregion_number="1"
                     /tool="antismash"
     gene            1..675
                     /gene="slvF"
     CDS             1..675
                     /codon_start=1
                     /gene="slvF"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 177.7; E-value:
                     5.1e-54)"
                     /gene_kind="transport"
                     /product="putative lantibiotic transport ATP-binding
                     protein SlvF"
                     /protein_id="AEX55154.1"
                     /transl_table=11
                     /translation="MQININQLSKSYKDKTILDNVTFTIESGTICGLLGVNGAGKSTLM
                     KILFGLVPKSDGEIQFDGLSKNKQSLGALIETPAIYHNLSAYDNLKTKALLYDIPDSKI
                     FETLEIIGLSNTGKKQAGNFSLGMKQRLGIGMAILTNPQFLILDEPTNGLDPDGINELL
                     ELIRSLKAKGMTILVSSHQLLEISKVADHILILDKGRIQYDGENKNVDSLERLFFKIVH
                     GG"
     misc_feature    1..15313
                     /note="salivaricin D gene locus"
     region          1..15313
                     /contig_edge="False"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            665..1408
                     /gene="slvE"
     CDS             665..1408
                     /codon_start=1
                     /gene="slvE"
                     /product="putative lantibiotic transport membrane protein
                     SlvE"
                     /protein_id="AEX55155.1"
                     /transl_table=11
                     /translation="MGVSDMQKIFYSELLKLRHLGTTKFLIIIPFLTVLIAFLMGGLQI
                     FVPFSVYWWEAIFLYLVNGILFIVDRRLEDQAGHFQNVTNGYFTWKISLMKMVLIGMRT
                     LLASFFLLAFLSLVAFIYTGFIPINIPKTAISLILIWMTTLWNIPLLYLMSKYMSQFIL
                     LALNTLVCLLIAPFIAQSPLWFVFPYTYHYKIAQFLLNLKPSGDIITNQNQTSILQVSL
                     IIILSMIMTLLLSYVLKRRESNATE"
     gene            1395..2045
                     /gene="slvG"
     CDS             1395..2045
                     /codon_start=1
                     /gene="slvG"
                     /product="putative lantibiotic transport protein SlvG"
                     /protein_id="AEX55156.1"
                     /transl_table=11
                     /translation="MLRSELLKLKNTTCLYLIISFSILQTLSIPLYVRFVPNGISLTNL
                     AILSFLCYPLLTAFLSILGIEQEKLANHYQEISSYPKKRLLWLVKLLITDLALTLPSLF
                     GWLIINLLLKNWINGLLLMISSWMLIVFLNHFHYFIQVCLSSTSNIVISIVEIIFIIFA
                     SNKVFLTIHWLPFTFPINSVLTTEWTPLNTLSYWLVGITLLFIYFVDFKVKVE"
     CDS             2821..3705
                     /codon_start=1
                     /product="transposase"
                     /protein_id="AEX55157.1"
                     /transl_table=11
                     /translation="MRQLTELCDFSFIYDELEKNFRPDFGRKTYSSIMMFKYLLLKDIY
                     KLSDVESFSNMAFKYFLGLSPEDSVIEPSSLTKFRKLRIKDERLLDLLITKSVQIALKH
                     SLIKSKILIMDATILKLTTIIRNPKRFSVNTQKSCVKQFTNAQKISKKFPSKPQEDNFE
                     DELRYIQELVSVLEKHEELITLLAVSQKLNYLKGAIEDDLEHLGSSVKEEDRLDHKSAD
                     SSFCGYKEHLAMTDERVITACVVTSSEKSDNPVLEELYHKSKNNGVSIEAIVGDKAYSG
                     KDNIQFTQKECVH"
     gene            complement(4383..5720)
                     /gene="slvK"
     CDS             complement(4383..5720)
                     /codon_start=1
                     /gene="slvK"
                     /gene_functions="regulatory (smcogs) SMCOG1003:sensor
                     histidine kinase (Score: 114; E-value: 1.7e-34)"
                     /gene_kind="regulatory"
                     /product="putative sensor histidine kinase SlvK"
                     /protein_id="AEX55158.1"
                     /transl_table=11
                     /translation="MFKRYSIRQRIWQFIVEIITGSLLVITLTMGMGVLLKQTGQLALP
                     SSSSFSVDLGDASISAINQKMSHIPYDYVIFDKKSGNILGGTYQKSDLLAYKLANNNSG
                     DVEKKGVTYTYASNEAVSIVVRHSTLPEFTNARLRHISYNKFSYVTVIVGVFLTIVISV
                     YRLAREYQANFKEIQKLALNMGKSTLDFNNHLKILEFNNILNNLTEKNNELVYLINKER
                     HEKEDLSFQVGALSHDVRTPLTVLKGNIELLETTIISKEQSEFLEAMKRSLLRFDNYFS
                     QMMDYTKLLNEDDHKEYISLELFLSDTCLLLNEFANTYGVSYHEDIQVYNLDIYINSHA
                     LSRAITNIFVNACQYARKGSKQVYLNVYLKQDRLCFSICNNGEPFSESAKKNASKLFYT
                     EDSGRTGKHYGIGLAFAKEVAFRHDGILELRNPPGGGAEVILQIKV"
     gene            complement(5713..6399)
                     /gene="slvR"
     CDS             complement(5713..6399)
                     /codon_start=1
                     /gene="slvR"
                     /gene_functions="regulatory (smcogs) SMCOG1008:response
                     regulator (Score: 156.8; E-value: 1e-47)"
                     /gene_kind="regulatory"
                     /product="putative response regulator SlvR"
                     /protein_id="AEX55159.1"
                     /transl_table=11
                     /translation="MHKILIVDDDIDILKMMESALRLKNYKVETRSEINVPVRTQEFQG
                     FDVILLDIMMANIEGTDICKCIRDSISTPIIFVSAKNDERDILTGFDVGGDDYITKPFS
                     LKQLVAKVEANIKREERRRGIHQTFTDVRRDFGPLTFYLEENRVCIDGKTIPFTSREYD
                     ILELLSSHPRKVFTIDDIYTRVYDEDADALLRSISEYIYQIRKKLSCYGVEPIKTVRGK
                     GYQWNV"
     gene            complement(6374..7990)
                     /gene="slvP"
     CDS             complement(6374..7990)
                     /codon_start=1
                     /gene="slvP"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075:alkaline serine protease, subtilase family
                     (Score: 227.9; E-value: 4.8e-69)"
                     /gene_kind="biosynthetic-additional"
                     /product="putative serine protease SlvP"
                     /protein_id="AEX55163.1"
                     /sec_met_domain="Peptidase_S8 (E-value: 2.2e-44, bitscore:
                     141.6, seeds: 43, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKRIIVLLFLVTCIGTVGVSYADTEDEPGGPIVSTKVFTDGELQN
                     KRENSSENKNINSDSNRLNDNSNQTGLRDDLSTDSDNADSTLINEESQSFWNYQWDMQY
                     VTDNGKSYEQYTPSKDISVGVIDSGITLDHPDLTNSLGSHLENFVPQNGFHGEEQEETG
                     VLNYVNDKLGHGTEVVGQITANGRVKGVSPGIIVNIYRVFGDSLADPKWINRAIRKAAD
                     DGNKVISLSMGQYLLISGSYDDGTNDLETYVIYKDAIDYASEKGSIVVASLGNEGLNEQ
                     DNSQMLHYLSSFHHVKEAGIVVDAPCIFDQVVAVGGLDMYGNISDFSNFTDKAIYAPAG
                     STINLKKYGPTEFLNKGFYLKDWIFTTSYTGWYQYVYGNSFAAPKVASAIALIADKYHI
                     TSPEQLKQFLFKNSPTINGNKVLKLTDLLNVGNADMFLYMSIENSISNFNIYSKSSVDR
                     SKITLQLKDVPKQLSYIITDTSIDHLNNKMLPPMGEKVGSNILLIGFLMIDLSALLIFN
                     NLIIKYNSDKEVESAQNSYCR"
     gene            complement(7992..8726)
                     /gene="slvI"
     CDS             complement(7992..8726)
                     /codon_start=1
                     /gene="slvI"
                     /product="putative immunity protein SlvI"
                     /protein_id="AEX55162.1"
                     /transl_table=11
                     /translation="MGRRTSIIVSILLVLLTGCGLNNTEKIEFSKTDFRHITFNHKDYS
                     ITDNEISKDNIRNVLVKDYKVIWINSHTGKILSKVSNNSCTLAIMNIFQTSDKLLCIGI
                     DDKYFQIAPKDQTSNLLNISRFSSKNTNVELDRGDDILIDDNNCRRIFYRGEAYYITSE
                     EVSSDKVGVYIDNLAEVRLFDAETGKKISNKERYAIYNKGEAKDRVNWFYGEVYSIKNS
                     KDDEEFLVVKINNVYRRAMKEE"
     gene            complement(8739..9959)
                     /gene="slvC"
     CDS             complement(8739..9959)
                     /codon_start=1
                     /gene="slvC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: LANC_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1140:Lanthionine synthetase C family protein (Score:
                     279.8; E-value: 5.9e-85)"
                     /gene_kind="biosynthetic"
                     /product="putative lantibiotic cyclase SlvC"
                     /protein_id="AEX55161.1"
                     /sec_met_domain="LANC_like (E-value: 1.5e-63, bitscore:
                     204.4, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTELKQQAIEKIIEKWDRKLIENRNEMDFTELTFSSGIPGVVLL
                     LAEYGLREGFPNYSNKIETYIEYMVYKLSRYGMSTGSIYSGASGIALSILHLQEKPNYH
                     NLFNSLNDYIVYYVEEQIKSIDIDEISPLDYDIIEGLAGILNYLLLLNDSHYDELKVKI
                     VNYLSNLANMEDKLLKFYIKSNNQMSELENNVYPTGCLNLGLAHGLSGVGCVLCYAYRK
                     GFGNNQTLIAIKNILNIYEDFELTDWQPFIWKDSLTKEEITTNKIIHQANFIRDAWCYG
                     SPGISLFYLYSGLILNDTYLKDKAEKILKASMDRLYGVDTNMLCHGYTGIIQICSLFKK
                     ILGTNSFNNYIEEFIGKEDDVIKSFGDDELEFLEGLSGCLLAFNELEDSTKLSYWKQPL
                     LLFEDFS"
     gene            complement(9916..11754)
                     /gene="slvT"
     CDS             complement(9916..11754)
                     /codon_start=1
                     /gene="slvT"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 165.8; E-value:
                     2.2e-50)"
                     /gene_kind="transport"
                     /product="putative ABC transporter SlvT"
                     /protein_id="AEX55160.1"
                     /transl_table=11
                     /translation="MDKIESLTAKQFIRTILTIPNTLKLIFNIEKRYAIYLIILNVLTA
                     MSSLVSLIIFQELINSIYLSSSYLIFVVIIYFILQILISLLGQVESYIDGKFNFNLSYS
                     IDRKLMETINSLELSELEQSDMYNIIEKITQESTYKPYQLFNAILQVLSSVVTMISSLI
                     FIATWNISMAMLLGVIPIISLVMFLRIGQLEFLVQWERANEERETWYIRYLLTHDFSFK
                     EIKINDLSSYFIEKFSKFKQSFIKQDLKIARKKAYFNIILGIILNTINLFIALSIIIYV
                     RAGKLLIGNFVSLIQVVARVNSSSQSLIQNIYIVYNTSLFIEQFFEFMETNNVKKENKQ
                     ANITHLNKLDEINIHQLNYRYPNTEDYALKDINLSFTRGELVAIVGGNGSGKSTLVKLI
                     SGLYTPSSGVIEYDGMSGKFLDDTFYKENISVLFQDYVKYELSLRENIGLSDTNSMRKD
                     HEIIQLLEKLGLDFLRVNGSYDLDLQLGTWFRNGRQLSGGQWQKIALARTFFKKSSIYI
                     LDEPSSALDSISQKEIFDHFIELSKNDISIFVSHDLEAAKIADRIIVMRKGKVEDIGTH
                     SELFSRCKYYKELYESQQYEEINEYGIKTTGYREDY"
     gene            complement(11764..14718)
                     /gene="slvB"
     CDS             complement(11764..14718)
                     /codon_start=1
                     /gene="slvB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lanthipeptide_LanB_RRE"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1155:Lantibiotic dehydratase domain protein (Score:
                     740.5; E-value: 4.4e-224)"
                     /gene_kind="biosynthetic"
                     /product="putative lantibiotic dehydratase SlvB"
                     /protein_id="AEX55164.1"
                     /sec_met_domain="Lant_dehydr_N (E-value: 5.1e-92, bitscore:
                     298.7, seeds: 73, tool: rule-based-clusters)"
                     /sec_met_domain="Lant_dehydr_C (E-value: 2e-21, bitscore:
                     66.5, seeds: 35, tool: rule-based-clusters)"
                     /sec_met_domain="Lanthipeptide_LanB_RRE (E-value: 2.8e-10,
                     bitscore: 30.7, seeds: 20, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINSFEARDFLVRNTYCSYKNEEFISYQQLVENCLSDQVFLEQLA
                     LANPKLFETLKRYSFGNLKKKREKSLFSSIFKYYKRSFQRSTPFGLFSETSIGKFAEEG
                     QSSLSSNTIKCVSLDSQWLIELVYKLENEFYESLSFSINGSNFESGDRIFQLYTVNSTD
                     LEEINIKLTNVYKIIEEACRDKFCSYSVILDKILKEYGLEYRSLAEQYILGLIKNHYLL
                     SNLQKDLVSRFNQENFIRILQNIDYEDKYTSSFKQIVSLINEYKTLKIGKGTNLLLDIY
                     QKMSSLVENENYLQVDLLSDSKVKLNVQHKKELERFANFIGNTQKSVRRSYLDDYRDKF
                     IEKYGVDQEVPLLELLDPTFGIGAPYGYVHPKSNFYENEPLTEFYSTQEQSKYLSEYIH
                     SIRENIDIDLEKFEDYYQLEENNKFNLQGLELFFNIVEINGKLVFSLSNIVGGSDIGSA
                     SGRFSILSDELRKYQKSLLDFIEEEDSTKKITSCEIEFIPENLRHRNIMRTMNVRDKTL
                     SLFTNNSKKQIHLKDIYIGINNEEKFYAKDITNNDIVKFHVTNMYNQMLFCNEIRFLYE
                     ISLDIDSINLPWELIYSNFDYVPRIMFGDIIVAPARWKICKGDIEKLSDINTFFINKRI
                     PQKFYLINGDNRILLSRKDKLDDEFIKNELIKKLKKDSIVELQEYIQDFGIFTKEVTDR
                     VADVVIPFVNNVKKDIDITAHAKRIDIESREKLPFDEWLYLKLYIGDNRQNEFIKEYLP
                     NIQEVVDSYQGELFYLRYADPNSHIRLRMKCDNLFDLYKQILNIISEGRKNRLISSVDI
                     STYDREIERYGGEDLLLEAEKIFCTDSRLMPRLLKICETNEMGFNLDSLSIVSVYLYLV
                     FFFDDDLHEIISFLETVSPKRNDKNKDINSDVKEYQKIIEANCNEKFFLCLKNPIKSLN
                     NKMLLNKLTKQQHYSIIDSIIHVHNNRLIGIDREKEKYIYFVLRRIFISKIHIK"
     gene            complement(14830..14991)
                     /gene="slvN"
     CDS             complement(14830..14991)
                     /codon_start=1
                     /gene="slvN"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR03731"
                     /gene_kind="biosynthetic-additional"
                     /product="putative lantibiotic SlvN"
                     /protein_id="AEX55165.1"
                     /sec_met_domain="TIGR03731 (E-value: 9.8e-14, bitscore:
                     41.0, seeds: 23, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MATKDFNLDLVEVSKSNTGASTRITSHSFCSQACKTYKILGCNLL
                     THGCRFPR"
     gene            complement(15140..15313)
                     /gene="slvD"
     CDS             complement(15140..15313)
                     /codon_start=1
                     /gene="slvD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR03731"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) mature_a"
                     /gene_kind="biosynthetic-additional"
                     /note="SlvD"
                     /product="salivaricin D"
                     /protein_id="AEX55166.1"
                     /sec_met_domain="TIGR03731 (E-value: 1.6e-20, bitscore:
                     62.7, seeds: 23, tool: rule-based-clusters)"
                     /sec_met_domain="mature_a (E-value: 5.7e-08, bitscore:
                     21.9, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTKDFNLDLVEVSKSNTGASARFTSHSLCTPGCITGVLMGCHIQ
                     SIGCNVHIHISK"
ORIGIN
        1 atgcaaataa atattaatca actttcaaaa tcctataaag ataaaactat cttggacaat
       61 gtcactttta ccattgaatc gggtacaatt tgtggtctat taggggttaa tggtgctggt
      121 aagtcaactc tgatgaaaat tctctttggt ttggttccaa aaagtgatgg agagattcaa
      181 tttgatggat tatcaaaaaa caaacaatca ttaggcgcac taattgaaac acctgctatt
      241 tatcataatc tttcagccta tgacaattta aaaacgaaag cgctattata tgacattcct
      301 gattcaaaaa tttttgaaac cttagaaata atcggtcttt ctaacaccgg gaaaaaacaa
      361 gcgggtaatt tttcattagg tatgaagcaa cgacttggga ttggtatggc tattttaact
      421 aatccccaat ttttaattct agatgagcct actaatggac ttgatcctga tggtataaat
      481 gaattactag aacttattcg ttccttgaaa gcaaaaggga tgacaattct ggtttccagt
      541 catcaacttc tagaaattag taaggttgct gatcatattc ttattctcga caaaggtaga
      601 attcaatatg atggtgaaaa caaaaatgta gatagtcttg agagactctt ctttaaaatt
      661 gttcatgggg gttagtgata tgcaaaagat attctattcg gaattgctca aattacggca
      721 tttaggaacg actaaattct taatcatcat accattttta accgttctta tagccttttt
      781 aatgggaggg ttacagattt ttgttccttt ctcggtttat tggtgggaag ctatcttttt
      841 atatcttgta aatggtattt tgttcatagt agatagacgt ttagaagacc aggctgggca
      901 ttttcaaaat gttacaaatg gatatttcac ttggaaaatt agcttgatga aaatggtgct
      961 tataggcatg agaacattgc ttgctagttt tttcttacta gctttcctca gtctcgttgc
     1021 atttatttac acaggtttta ttccaataaa tattcctaaa acagctattt ccttaatctt
     1081 aatttggatg acaactttat ggaacattcc tttactttac ctaatgtcta aatacatgag
     1141 ccaattcatc ttattagctc ttaatacatt agtttgtctt ttaatagccc cctttattgc
     1201 tcagagtcca ctctggttcg ttttccccta tacctatcat tataaaatag ctcaattttt
     1261 attaaacctt aaaccatcgg gcgacattat tacaaatcaa aaccaaacaa gcattttgca
     1321 agtgtcactt attattattt tatcaatgat aatgacatta cttttatcat atgtcctaaa
     1381 aaggagggag tcaaatgcta cggagtgagt tacttaaatt aaagaatact acatgtctct
     1441 atcttattat cagtttttct atactacaaa ctctttcaat tcccctctat gtaagattcg
     1501 tccctaatgg catttctcta actaatctag ctatcttatc ctttttatgt tatcctctat
     1561 tgacagcttt cttgtccatt ttaggaattg aacaagaaaa actcgctaac cattatcaag
     1621 aaatctctag ttaccctaag aaaagactcc tgtggctagt aaaactttta ataacagact
     1681 tagcacttac cctaccttct ttatttgggt ggttaatcat aaacctcctc cttaagaatt
     1741 ggattaatgg tctcctgcta atgatatcaa gttggatgct aattgtcttt cttaatcatt
     1801 ttcattattt tatacaagtt tgtctaagta gtacgagtaa catcgttatt tctatagttg
     1861 aaatcatttt tattattttc gctagtaata aagtcttctt aactattcat tggcttcctt
     1921 ttacttttcc tattaacagt gttctcacta cagagtggac tccactcaac actctttctt
     1981 attggcttgt agggatcacg ctattattta tctattttgt agattttaaa gtcaaagtag
     2041 aatgagataa tcttctaaat tacttagaat aaaaagcaaa atccaagaaa tactggctct
     2101 gtaattgata tagtggggaa gacactgtgt agatgaacta cacccccaaa attagatttt
     2161 tttctgtcta acttttggga tgtttttatt atgaaattga cttataagga caaaggtcag
     2221 atttataaac ttagaaagta aggaggaagc ttccagagac cttcgaatca atttggggta
     2281 gatgtttgct gttctaaagt acatggtgag atcaatcgac cgttatggaa taagacccgt
     2341 caaaaagaaa ggaatcgtta ctattctcct gaattaaaca aaaaatcctt gataaagttc
     2401 tacttgaagg ccattcataa agaaaaacta aaaaagtaga tgagtttttg aactcattca
     2461 agaaaacata aacgacgtca atcattactt tcaaactatc tttagggctt ttcttagata
     2521 caaataatac atcaaaaatg cactagaaac agactattca aacccgaaac tcgaagcaac
     2581 taataagttg atcaaagaca tcaaacgctt gggcttcggt tttagaaact tcatcagctt
     2641 taagaagcgt gtattcatga ctctgaacat aaaactagcc gggagcccac tgaaaaagtc
     2701 atcaatcgat ggcttttttt atactaaact cgaggtgatt gtatacttga taatcagtta
     2761 actttagaag ttagtcccta ttccagtcta tatgatattg ttgttctaaa gaatcatttt
     2821 ttgcgtcagc taacggaact ctgtgatttt agctttattt acgatgagtt agaaaagaat
     2881 tttcgtcctg actttggacg taaaacttat tcgtcgatta tgatgttcaa atatctctta
     2941 ttaaaagaca tctataagtt atccgatgtg gaatcctttt ctaacatggc ttttaaatac
     3001 ttccttggtc tatctccaga agatagcgtt attgaaccct cttcattgac taaatttcgc
     3061 aaacttcgaa tcaaagatga acgattactt gatttactca tcactaaatc cgtacaaatc
     3121 gctcttaagc atagcttgat taaaagtaaa attctcatta tggatgcgac catactaaag
     3181 ctcactacaa tcataagaaa ccccaagagg ttctccgtga acactcaaaa gtcttgcgta
     3241 aaacaattta ccaatgctca gaagatatca aagaagtttc ctagcaagcc tcaagaagat
     3301 aattttgaag atgaattaag atatatacaa gagttagtat cagtcctaga aaaacatgaa
     3361 gaattgataa ctttacttgc ggtttcgcaa aaattaaatt accttaaagg agctatagag
     3421 gatgatttag aacacttggg atcctctgtt aaagaggaag atcgtcttga ccataaatct
     3481 gcggatagtt ccttttgtgg ctataaagag catctcgcca tgacagatga gcgtgtgatc
     3541 acagcctgtg tcgtgacatc cagtgaaaaa agtgataatc ctgtcttaga agaactctat
     3601 cataaatcta agaataatgg tgtaagcata gaagctattg taggggataa ggcctattct
     3661 gggaaagata atattcaatt tacccaaaaa gaatgtgtgc attaagtcgc taagttacat
     3721 cctgctgttt caagaggctt tcgtcggaaa gaagatgaat tcttctatca acaaggatgc
     3781 aggtctcttt gtttgtccag aaggacatat ggtactaaag aaagctaaga taggaagaac
     3841 aacggggaaa aataattctc aagaaaccca ctgttttgat attaagtatt gtcaaatcta
     3901 cccttcaaaa tgcagaactt aaacacagac atagtcttga tatggttaga gcatcaggtc
     3961 ttttcaatat ggtgttacaa gcgaaacaac tatatttggg gtcaacatga agaggattat
     4021 aactttaata aattcaaaat aagtaaacaa aaggagaaat ctatctcata tttaggtaga
     4081 tttctccttt ttattatatt taaactgtag agattacttt ttcagtgggc tccctatcct
     4141 ccctatccgg tcttctctag atgttagctt ttcgttaccc actacagttg acagagagcc
     4201 gccaaaataa gacagcgact tcagaaaagc tactgtcata tttcatgata gacttgacaa
     4261 atggtagaaa agagccactt aaattatttg tctaattttt tggagtcagc agatacttga
     4321 gagcactttt atctaaaata tctcttttga gtagtttatc atttgcttca acctttctcg
     4381 tatcatactt taatttgtaa aatcacttct gctccacccc ctgggggatt tctcaactct
     4441 aaaattccat catgtctgaa agctacttct ttagcaaaag ctaagcctat cccataatgt
     4501 ttaccagtcc ttccagaatc ctctgtataa aatagcttac tcgcattttt ttttgcactt
     4561 tcagaaaatg gttccccatt attacatatt gaaaagcata acctatcttg tttcaaatat
     4621 acgtttaaat aaacctgttt actacctttt ctagcatatt ggcatgcatt tacaaaaata
     4681 ttagttatcg ctcttgacaa agcatgacta ttgatataaa tatctaagtt atagacttga
     4741 atatcttcat gataggaaac accatacgta ttagcaaact catttaataa taagcatgta
     4801 tctgataaaa ataattctaa cgaaatatac tccttatgat catcttcatt taataattta
     4861 gtataatcca tcatttgaga aaaataatta tcaaatcgta acaatgaacg cttcatagct
     4921 tctaaaaact cactttgttc cttagaaata atcgtcgttt ccaaaagctc tatattaccc
     4981 ttcagaacag tcaagggtgt tcttacatca tgtgaaagtg ctcctacttg aaatgataaa
     5041 tcttcttttt catgtctctc tttatttatt aaatatacta attcgttatt tttttctgtt
     5101 agattgttca aaatattgtt gaattctaat atttttaaat ggttattaaa atctagagtt
     5161 gattttccca tatttaatgc tagtttttga atttctttaa aattagcctg atattcccta
     5221 gctaaacgat ataccgaaat aacaattgtt aagaaaactc caacaattac agttacatag
     5281 gaaaacttgt tataactaat atggcgtagt ctggcattgg taaattctgg aagagtgcta
     5341 tgtcttacaa ctatagatac tgcctcgtta cttgcatagg tataagtaac cccctttttt
     5401 tcaacatctc cactattgtt atttgcaagt ttatatgcta gtaaatcact tttttgatag
     5461 gttccaccca aaatattacc tgatttttta tcaaagatta catagtcata aggaatgtgg
     5521 ctcatttttt ggttaattgc tgatatagag gcatcaccca aatcaaccga gaaactcgaa
     5581 gaagatggta aggccagttg tcccgtttgt ttcaacaata ctcccatacc catggtaaga
     5641 gtaatcacaa gaagagaacc agttatgatt tcaacaataa attgccaaat acgttggcgt
     5701 atagagtatc gtttaaacat tccactggta ccccttacct ctaacggttt ttataggttc
     5761 tacaccataa catgatagtt tttttcttat ttgataaata tattccgaaa tagaacgaag
     5821 aagtgcatcc gcatcctcat cataaacacg tgtataaata tcatcaattg taaatacttt
     5881 tctaggatga cttgataaaa gttccaaaat atcgtattcc ctactagtaa agggaatcgt
     5941 tttaccgtca atacaaacac gattctcttc gagatagaag gttaaaggtc caaagtcacg
     6001 tctaacatca gtaaaggttt ggtgtatccc cctacgacgc tcctcacgct taatattcgc
     6061 ttctactttg gcaactagct gttttaaact aaagggttta gtaatgtagt catcaccacc
     6121 tacgtcgaat ccagttaaaa tatctctctc atcattttta gcactaacaa aaattattgg
     6181 agttgaaata gaatctctga tacatttaca gatatctgtc ccttcaatgt tcgccatcat
     6241 aatatctaat aatataacat caaatccttg aaattcttga gttcgtactg gaacattaat
     6301 ttctgatcgc gtctccacct tatagttttt taatcgaaga gctgattcca tcatttttaa
     6361 aatatcaata tcatcatcta caataagaat tttgtgcact ttctacctcc ttatcactat
     6421 tatactttat aattaaatta ttaaaaatta ataacgcaga caaatcaatc atcaaaaaac
     6481 caattagcaa tatattagag cctaccttct cacccatcgg aggcaacatt ttattattca
     6541 gatgatcaat actagtatcg gtaataatat aagaaagttg ttttgggaca tcctttaatt
     6601 gaagtgttat ctttgaacga tcaacactcg acttagaata tatattaaag tttgatatac
     6661 tattttctat actcatatag agaaacatat ctgcatttcc cacattcaaa agatcagtta
     6721 attttagtac cttatttccg ttaatagtcg gtgagttttt aaaaagaaat tgtttcaatt
     6781 gttctggtga cgtaatatga tacttatctg ctattaatgc tatagctgaa gcaactttcg
     6841 gagcagcaaa agaatttcca tacacatact gataccaccc tgtataacta gttgtaaaaa
     6901 tccaatcttt taaatagaaa cctttattta aaaattctgt aggaccatac tttttaagat
     6961 taattgttga acccgcagga gcatagattg ctttatcagt gaagttacta aagtctgaaa
     7021 tattcccata catatcgagt cctccgaccg caacaacctg gtcgaatata caaggcgcat
     7081 ccactactat ccctgcttct tttacatgat gaaaactgct aagataatgc aacatttgtg
     7141 aattatcttg ttcatttaaa ccctcattcc ctagggaagc aactacaatg gatcctttct
     7201 cacttgcata atcaatagca tctttatata taacataagt ctccaaatca ttcgttccat
     7261 catcataaga acctgaaatt aataaatatt gtcccatact aagtgaaata actttattac
     7321 catcatcagc tgcttttctt attgccctat taatccattt tggatcagca agtgaatctc
     7381 caaagacacg ataaatattt acaatgatac ctggggagac tcccttaact cgtccattcg
     7441 ctgttatctg ccctacaact tcggtaccat gtcccaattt atcattgaca taatttagca
     7501 cccctgtttc ttcttgttct tcaccatgaa aaccattttg gggaacaaaa ttttctaaat
     7561 ggctacctaa actattagtt aaatccggat gatccaaagt aatacctgaa tcgattactc
     7621 caacagaaat atccttagaa ggagtgtatt gttcatagct tttaccatta tcggtaacat
     7681 actgcatgtc ccactgataa ttccaaaaac tctgactctc ttcatttatc agtgtactat
     7741 cagcattatc actatcagtt gacaagtcat cccttaagcc agtttgatta ctattatcat
     7801 ttaatcgatt tgaatctgaa ttgatattct tattttctga agagttttcc ctcttgtttt
     7861 gtaattctcc atctgtaaat acttttgtag aaactatcgg accacctggt tcatcttcgg
     7921 tatctgcata gcttactcct actgtcccaa tacaagttac caaaaacaat aaaactatga
     7981 ttcttttcat cctactcttc cttcattgca cgcctgtaaa cattattaat cttaaccaca
     8041 aggaactctt catcatcttt tgaattcttt atggaatata cttctccata aaaccaattc
     8101 actcgatctt tagcttctcc cttattataa attgcataac gctccttatt cgaaattttc
     8161 tttccagtct cagcatcaaa gagtctgacc tctgccaaat tgtcaatata aactcctacc
     8221 ttatcactcg atacttcttc acttgttatg taataagctt ctcctcggta gaatattctc
     8281 ctacaattat tatcatcgat taaaatatcg tctcctctgt ccaattcaac atttgtgttt
     8341 ttgctactga atctagatat atttaacaga ttagatgttt gatctttcgg agcaatttga
     8401 aaatacttat catcaatacc aatacacagt agtttatcac ttgtttgaaa tatattcatt
     8461 attgccagtg tacaactgtt atttgatact tttgagagaa tttttcctgt atgagagtta
     8521 atccaaatca ctttatagtc ttttaccaaa acattcctta tattatcttt agaaatttca
     8581 ttatcagtga ttgaataatc tttgtgatta aacgttatat gtctaaaatc tgttttagaa
     8641 aactctattt tctccgtatt atttagtcca catcctgtca acaatacaag taaaatagac
     8701 actataatgc ttgtccgtcg tcccatttag attcctttct agctaaaatc ttcaaaaagt
     8761 agtaatggtt gtttccaata tgacaatttt gtcgaatctt caagttcatt aaaagcaagt
     8821 aaacatccac tcagtccttc aagaaactct aattcatcgt ctccaaaact ctttattacg
     8881 tcatcctctt tcccaataaa ctcttcaata tagttattaa aagaattcgt tccaagaatt
     8941 tttttaaata atgaacagat ttgaattatt ccagtataac catgacaaag catatttgtg
     9001 tctacaccat ataatctatc catagatgcc tttaatattt tttccgcctt gtcttttaag
     9061 taggtatcat ttaaaattaa tccactatat aagtaaaata aacttatacc tggactacca
     9121 taacaccacg catctcttat aaagtttgcc tgatggataa tcttattggt agtaatctct
     9181 tccttagtta aagaatcttt ccaaataaac ggttgccaat ccgtcaattc aaaatcttca
     9241 tatatattga gaatattctt aattgctatt aaagtctgat tattcccaaa tcccttccta
     9301 tacgcatagc ataaaacgca accaacacca gaaaggccgt gagctagtcc taagttaagg
     9361 catcccgttg gatatacatt attctccaat tcagacattt gattatttga tttgatataa
     9421 aattttaata gcttatcttc catattagct aagtttgata agtaatttac aatctttact
     9481 ttcagctcat catagtgaga atcgtttagg agaagtaaat agttcagtat tcctgccaat
     9541 ccctcaataa tatcataatc tagaggtgag atttcatcaa tatcaataga tttaatttgc
     9601 tcctcaacat aatatactat atagtcatta agactattaa aaagattatg ataattaggt
     9661 ttctcttgta aatgaagaat acttaatgct attccgctag ctccggaata aattgaacca
     9721 gtcgacatac catatctgga taatttataa accatatact cgatgtatgt ttctatttta
     9781 ttgctataat ttggaaaccc ctctcggaga ccatactccg ctagtaagag aacaactcct
     9841 ggtataccac ttgaaaatgt tagctccgtg aagtccattt catttctatt ttcgataagt
     9901 ttacgatccc acttttcaat aatcttctct atagcctgtt gttttaattc cgtattcatt
     9961 tatttcctca tactgctgtg attcatatag ttccttataa tacttacatc gtgaaaataa
    10021 ttcactgtgt gttccaatgt cctcaacttt tccttttctc atcacaataa ttctatctgc
    10081 tattttagct gcttctaaat catgtgatac aaaaatagag atatcatttt ttgacaactc
    10141 aatgaaatga tcaaaaattt ctttttgaga tattgaatca agggccgaac ttggttcatc
    10201 taaaatatag atggatgatt ttttaaagaa agttcttgct agagcaattt tttgccactg
    10261 ccctcctgac agttgtctac catttctaaa ccatgtacct aattgtaaat caagatcata
    10321 gctaccatta actctaagaa aatctagtcc caatttttct aataattgaa taatttcgtg
    10381 gtcctttctc atcgaattag tatcactcag tcctatattc tctcttaatg ataactcata
    10441 cttaacataa tcttgaaaaa gtactgatat attctcttta tagaatgtat catccaaaaa
    10501 ttttccggac ataccatcat attctattac accacttgat ggtgtatata atcctgaaat
    10561 taatttaacc agtgtactct tacctgagcc atttccacca acaatagcta ctaattcacc
    10621 ccttgtaaag gataaattga tgtcttttaa agcataatct tctgtatttg ggtatctgta
    10681 gttaagttga tgaatattaa tttcatctaa cttatttaga tgcgtaatat tagcttgttt
    10741 gttctctttc tttacattat tagtctccat aaattcaaag aattgctcga taaataaact
    10801 tgtattatac actatataga tattttgaat taatgactgt gaggaactat tcactcgtgc
    10861 aacaacctgt attaaactaa caaaattacc tataagaagt tttcctgctc ttacataaat
    10921 aataattgat aaagcaataa ataaattaat tgtatttaat atgataccta agattatatt
    10981 aaaatatgct ttttttctag caatcttaag atcctgcttg ataaagcttt gtttaaattt
    11041 tgaaaatttc tcaataaaat aactacttaa atcatttatc ttaatctctt taaatgaaaa
    11101 atcatgagta agtagatatc ttatgtacca tgtctcccgt tcttcattag ctctctccca
    11161 ttgaaccaaa aattctaatt gaccaatcct caaaaacatt actaaagaaa taataggaat
    11221 aactcctaat aacatagcca tactgatatt ccatgttgca atgaatatca agctagatat
    11281 catcgtaact actgatgata gcacctgtaa gattgcatta aataattgat atggtttata
    11341 ggtgctttct tgagttattt tctcaataat gttatacata tcagactgct caagttcact
    11401 taactcaagg gagttaatcg tttccattaa ttttctatca atactatagg acagattgaa
    11461 attaaatttc ccatcaatat aactctcaac ttgacccaac aatgagataa gtatttgcaa
    11521 aataaaataa ataattacta caaaaattaa ataacttgat gataaataaa tggaattaat
    11581 caattcttga aaaataataa gactaaccaa tgaagacatc gctgtcaaaa catttaaaat
    11641 aattaaataa atcgcatatc ttttttcaat attgaaaatc aatttcaatg tatttggaat
    11701 agtaagtata gtcctaataa actgttttgc agtcaggctc tcgattttgt ccattatata
    11761 ctcctactta atatgtattt tactaatgaa tatgcgtcga agaacaaaat aaatatattt
    11821 ttctttttct ctatcaatcc caattaaacg attattatga acatgaataa tactatcaat
    11881 tatcgaataa tgttgttgct ttgtaagttt atttagtaac attttattgt ttaagctttt
    11941 tattggattc tttaagcata gaaagaattt ttcattacaa ttagcttcaa taattttctg
    12001 atactcctta acatcactat ttatatcttt atttttatca tttctcttag gactcactgt
    12061 ctctaagaaa gaaataatct catgaaggtc atcatcaaag aagaatacaa gatacaaata
    12121 aacacttaca atggacagac tgtctaagtt aaaccccatt tcatttgttt cacaaatctt
    12181 caataatcgt ggcatcaatc ttgaatcagt acaaaaaatt ttttcggctt ccaacagcag
    12241 atcctcacca ccatatctct caatctcacg atcatatgta gaaatatcta cactcgaaat
    12301 caaacgattc tttcttcctt cagagataat atttaaaatt tgtttataca aatcaaataa
    12361 attatcacac ttcatgcgta gtcgaatatg tgaatttgga tcagcatacc taagataaaa
    12421 caattctcct tgatagctat caactacctc ttgaatatta ggtagatatt ctttaatgaa
    12481 ctcattttgc ctattatctc caatatataa tttcaaatat aaccattcat caaaaggtaa
    12541 tttttctcgt gattctatat ctattctctt agcatgtgca gttatatcaa tatctttttt
    12601 cacattatta acaaacggaa ttactacgtc agctactctg tcagtcactt cctttgtaaa
    12661 aattccaaaa tcctggatat attcttgtaa ttctacgata ctgtcttttt ttaatttttt
    12721 tattagctca tttttaataa attcatcgtc taatttgtct ttacgtgaaa gtaaaatcct
    12781 attatctcca ttgataagat aaaatttctg agggattcgc ttatttatga aaaaggtatt
    12841 aatatcagaa agtttctcga tatcgccttt acaaattttc catctagcag gagcaactat
    12901 aatatcacca aacataattc taggtacata atcgaaatta ctatatatta attcccatgg
    12961 taaattgata gaatcaatat ctaaagaaat ttcatacaaa aaacgaattt cattacaaaa
    13021 taacatttga ttatacatat tcgtaacatg aaactttaca atatcattat ttgttatatc
    13081 tttggcgtaa aatttttctt cattatttat tccaatataa atatctttca aatgaatttg
    13141 tttcttacta ttattagtaa ataaagaaag agttttatct cttacattca ttgttctcat
    13201 gatattccga tgtcttaaat tttcaggaat aaattcaatc tcacatgacg taattttctt
    13261 agttgaatcc tcttcttcaa taaaatctaa taatgatttt tgatatttcc taagttcatc
    13321 tgataaaatt gagaaacgtc ctgatgcact accaatatca cttccaccaa caatattact
    13381 taatgagaaa actagttttc cattaatttc gacaatatta aaaaataatt ccaaaccttg
    13441 caaattaaac ttattattct cttctagttg ataataatcc tcaaattttt ctaagtctat
    13501 atctatattt tctctaattg aatggatata ttcactcaga tatttacttt gctcctgagt
    13561 actataaaac tctgtaagcg gttcattctc gtagaaattg ctttttggat ggacataacc
    13621 atacggagca cctattccaa acgtaggatc taataattct aataacggaa cctcttgatc
    13681 aactccatat ttctcgataa acttatctct ataatcatcc aaatagctgc gtctaactga
    13741 cttttgtgta tttcctataa agttagcaaa acgttcaagt tccttcttat gttgaacgtt
    13801 tagtttaacc ttagaatcac ttaacagatc aacttgaaga taattttcat tttcaactag
    13861 actagacatt ttttgataaa tgtccaacaa caagtttgtt ccttttccga ttttaagggt
    13921 tttatactca ttaattaaac ttactatttg tttgaaagaa gaagtatatt tatcctcata
    13981 gtcaatattc tgaagtattc ttataaaatt ctcttgatta aatctcgaaa ctaagtcttt
    14041 ctgtaaatta gataataaat aatggttctt aattagaccc aaaatatact gttctgccaa
    14101 actgcgatac tcaagtccat actcttttaa aattttatct aagataactg aataactaca
    14161 gaacttatct ctacaagctt cttcaataat tttataaaca ttagttaatt tgatattaat
    14221 ttcttctaaa tctgtgctat tcactgtata aagttgaaaa atacgatcac ctgattcaaa
    14281 gttcgaacca ttaatactaa atgaaagtga ctcataaaat tcattctcta atttatagac
    14341 aagttctatt agccactggc tgtcaagtga aacacattta atagtattag aggataagct
    14401 actttgtcct tcttcagcaa attttccaat tgaagtttca ctgaataaac caaatggcgt
    14461 tgagcgttga aaactcctct tatagtactt aaaaatagac gaaaataaac ttttttctct
    14521 cttctttttt agattgccaa aactatatct ttttaacgtc tcgaatagct taggattagc
    14581 taaggctaac tgttctaaga acacctgatc tgataaacag ttttcaacta attgttggta
    14641 tgatataaat tcttcatttt tatatgaaca atatgtattt ctaactaaaa aatctctagc
    14701 ttcaaatgaa ttaatcaaaa taatgactcc tttattactt ttataaatta ttaaatacct
    14761 atttacagta acttataaaa ataggatagt gtaaaaatgc actatccaac aaaatatttc
    14821 agtgtcttcc tacctaggaa acctacatcc gtgtgttagt aaattacatc ctaaaatctt
    14881 ataagtctta caagcttgtg agcaaaatga atgactagta attcgagttg aagctccagt
    14941 attgcttttt gagacttcta ccaagtctaa attaaaatct tttgtagcca tttaagcacc
    15001 tccttaataa taattattat tatagtatag aatacttgct aattaagtat taatctgtta
    15061 ccatggtgtg tgtgtagtga ctttatatta ataaatattt gtaagataat atacagaatt
    15121 ccttatttct tgaattacgc tatttactaa tatggatatg aacattacat ccaatacttt
    15181 ggatgtgaca tcccataagt acaccagtaa tgcatcctgg tgtacaaagt gaatggctag
    15241 taaaacgagc tgaagccccg gtattgcttt ttgagacttc taccaagtcc aagttaaaat
    15301 cttttgtact cat
//
