LOCUS       BGC0000555             11750 bp    DNA     linear   BCT 21-MAR-2022
DEFINITION  Staphylococcus aureus TY4, ETB plasmid DNA, complete sequence.
ACCESSION   BGC0000555
VERSION     BGC0000555
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-07 19:51:26
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 11750
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..231
                     /codon_start=1
                     /note="unnamed protein product; ORF39; unknown"
                     /protein_id="BAB78437.1"
                     /transl_table=11
                     /translation="MYFDINTLDIKNLRDDKYQFLEDSVTINNAYFLAADEIAINILGE
                     LFSLFYSVNLVRFYMFIMSVKRKFFPNFIIN"
     protocluster    1..11750
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[1027:8839]"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1028..8839
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
     cand_cluster    1..11750
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(LANC_like and DUF4135)"
                     /kind="single"
                     /product="lanthipeptide-class-ii"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..11750
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000555"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..11750
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lanthipeptide-class-ii"
                     /region_number="1"
                     /rules="cds(LANC_like and DUF4135)"
                     /subregion_numbers="1"
                     /tool="antismash"
     CDS             594..782
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) leader_eh"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) mature_ha"
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /note="unnamed protein product; ORF40; lantibiotic
                     structural protein alpha Sac alpha A of Stapylococcus
                     aureus C55"
                     /protein_id="BAB78438.1"
                     /sec_met_domain="leader_eh (E-value: 6.9e-09, bitscore:
                     25.3, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="mature_ha (E-value: 4.4e-15, bitscore:
                     44.6, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKSSFLEKDIEEQVTWFEEVSEQEFDDDIFGACSTNTFSLSDYWG
                     NKGNWCTATHECMSWCK"
     CDS_motif       594..689
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence:
                     MKSSFLEKDIEEQVTWFEEVSEQEFDDDIFGA"
                     /locus_tag="BAB78438.1_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       690..782
                     /prepeptide="core"
                     /locus_tag="BAB78438.1_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="2.20"
                     /molecular_weight="3317.7"
                     /monoisotopic_mass="3315.4"
                     /core_sequence="CSTNTFSLSDYWGNKGNWCTATHECMSWCK"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MKSSFLEKDIEEQVTWFEEVSEQEFDDDIFGA"
                     /leader_location="[593:689](+)"
                     /alternative_weights="3335.7"
                     /alternative_weights="3353.7"
                     /alternative_weights="3371.7"
                     /alternative_weights="3389.7"
     CDS             806..1009
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /note="unnamed protein product; ORF41; lantibiotic
                     structural protein beta Sac beta A of Stapylococcus aureus
                     C55"
                     /protein_id="BAB78439.1"
                     /transl_table=11
                     /translation="MKNELGKFLEENELELGKFSESDMLEITDDEVYAAGTPLALLGGA
                     ATGVIGYISNQTCPTTACTRAC"
     CDS_motif       806..910
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence:
                     MKNELGKFLEENELELGKFSESDMLEITDDEVYAA"
                     /locus_tag="BAB78439.1_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       911..1009
                     /prepeptide="core"
                     /locus_tag="BAB78439.1_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="-1.70"
                     /molecular_weight="2956.4"
                     /monoisotopic_mass="2954.5"
                     /core_sequence="GTPLALLGGAATGVIGYISNQTCPTTACTRAC"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MKNELGKFLEENELELGKFSESDMLEITDDEVYAA"
                     /leader_location="[805:910](+)"
                     /alternative_weights="2974.4"
                     /alternative_weights="2992.4"
                     /alternative_weights="3010.5"
                     /alternative_weights="3028.5"
     CDS             1028..3925
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: DUF4135"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: LANC_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1070:lanthionine synthetase C family protein (Score:
                     612.4; E-value: 2.5e-185)"
                     /gene_kind="biosynthetic"
                     /note="unnamed protein product; ORF42; lantibiotic
                     modifying enzyme SacM1 of Stapylococcus aureus C55"
                     /protein_id="BAB78440.1"
                     /sec_met_domain="LANC_like (E-value: 6.1e-68, bitscore:
                     218.5, seeds: 47, tool: rule-based-clusters)"
                     /sec_met_domain="DUF4135 (E-value: 2.3e-69, bitscore:
                     223.7, seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVLFYKKEVYPELNKSDFKEHILPLNDFIEELSEPFSEFILEDSD
                     LIEKEELYPYHNACRIIAEGVWRHTWDTKLNKYTLNPSEFKKATCEFYSQVAFSYLIRS
                     FAKDIKLFKLSNDSNTDYDIYTKYLIENKFQDFSQTYNVVWGRCLKLLINRAKSIVKTI
                     ELINQNRNEIERIFDIPNSHKIVSINTSGDTHNNGSSVSVVNFEKELKLIYKPRPISGE
                     VGYSKLIEKLNCLVSSKFSSLKVLDLGGFGFTSYIEKNEMNKNMKEAGRIAALMYFLNS
                     SDMHYSNIYWTNNGPIPIDLETLFHPKRIADSNLENFNNAYYKIEKSVYGTGILPIHLT
                     KKSSDVSVDVGFTGVRDENSNSPFKTFNIEDGFTSNIKVVWQKQIEIDNSLYNDENYEK
                     FIYSNCKDLIEGFTEIYMIILNNKNYFINCVLETFDNSKLRYIHNMTYRYEQILRILTD
                     SEPSKNIDLAHMLLSRIGILSMTSNKNIVSSECTQLWNGDIPYFSISFDQKAVESENKF
                     VTWLPKSPKEEFMIKMNSISLEDLNRQIEIIKLAFVAKLADPHRDEQILESEINEDVYI
                     DKKTIEHSISYFMNSLTERIMNDRYNHLPKTWIGPVSQYGKGWTPGVLGYDLYSGRTGP
                     FLALLLSGKQLKNQKVIDVAYEFFDKVTKIFEEKTYDLRNLLMSGIGAFSGMSGIIWSL
                     YTAGKITENSKWKNTALKVFDLIDEEIKKEEKGDFFDMISGSSGAIIMRYKIQEDYSLS
                     ENFIKEKVKEAYNIINNKKNLSSGLAHGISQIMYFFAIIYKKQKLVYIRRIIEDIHKII
                     KSNFTNVQNLIEIYRSEDKNNISSSWCNGLSGLLVSYYEGYKAGIFSKDEVIDIIKQIE
                     IIPLSVVPILCHGSLGVYDSLKYVSKDFEKETRYLLMKLEKTTCSPAYIVNYFKEGRGR
                     YTLSPGLMTGKTGALLHLCKLLDGEIDVSPLTFEV"
     CDS             3927..6089
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288:ABC
                     transporter related protein (Score: 196.4; E-value:
                     1.4e-59)"
                     /gene_kind="transport"
                     /note="unnamed protein product; ORF43; transporter SacT of
                     Stapylococcus aureus C55"
                     /protein_id="BAB78441.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 7.2e-41, bitscore:
                     128.6, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAKLKIFNKKRVPPSLQIAQTECGLCCVKSILNYYDFSVSLPELR
                     KIKEPGRDGLGLKQIKGLLECYNFKVKNFKVKDNKAFDMLNFPLIAFWKGCHYVVLESY
                     NDKNVVIMDPSVGRIKISTLEFNANFDRYVILCTPNDNFQKRNISTVSKFKKTYIWPNN
                     MISIYVKLALLSLIAITITLSIPIATQKLIDFSLQTQKSLIFIVIIIIFSMIIAVFLSF
                     LRNLYSIQMILKFNWFLMNGAFKRLLYLPAKYFSVRSPGEITYRINALTRIQDIIGPTL
                     VRSILDFFSSCTLLGYVFFINPLIAILILIFLLLNFIFLAIMQKHINFSKDRELHEGSS
                     AQSIQLDALVSINSVKIGGYMKRYIDDWANRYQNLLKAMEKRMKLQDGLVGSILNGFQT
                     FAPLLILSINIYLSQVGYMTLGQAIAVQSVVSMLFTYTNSIFTTISEALTATKYIELSE
                     DIFEYPEENRINSMIQMQSGDVEIKNLHFSYTPESPLAIKSINLSIKEGDTIALVGMSG
                     SGKTTLGKLISTLFEPSSGSILYGGVEKTKYNIDNLRSSISYIPQEAYLHNRTILENLK
                     LGCNYNEYEIIKRCQSMPFLNFINSLPMGYSTVVSEMGANLSGGQRQRIHIAKVLLQSP
                     KLLVMDEATSSLDNVSQSHVYDELSRLKCTKIVIAHRFATVFNADKIVVMDHGEIVEVG
                     AHNELLNKNGLYRKLFNAEFKKECGV"
     CDS             6086..8839
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: DUF4135"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: LANC_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1070:lanthionine synthetase C family protein (Score:
                     503.9; E-value: 1.5e-152)"
                     /gene_kind="biosynthetic"
                     /note="unnamed protein product; ORF44; lantibiotic
                     modifying enzyme SacM2"
                     /protein_id="BAB78442.1"
                     /sec_met_domain="LANC_like (E-value: 1.3e-50, bitscore:
                     161.5, seeds: 47, tool: rule-based-clusters)"
                     /sec_met_domain="DUF4135 (E-value: 1.8e-86, bitscore:
                     280.0, seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKIKDVTNIITRAIEEININMLENNNIQIKKITEDIDDGDNINN
                     LIEQCRQSLIEINVWKITYKTFVYEFHEFRKKINYPISTNSSIAYNHYINNISAETLIH
                     WFNKYPYLREIIEKIINNICEFSLKVCHDLITDWNILIEKNLINKGEKLQNITCLDSDP
                     HNNNKVGLCFDFEKSKVLYKPKSLKVDMLISEIFENILQFEELNKIIPVATSIDRGDYG
                     WQRFINRNYLNKDDLPKAFYNLGLCSSLFTALGATDLHDENIIFNNEYPYFIDLETCLQ
                     PEIKYINNSLVDTMLDNIKSSIASTSIIPSNIISYLQQILVGAINTPYPQKTNKRRFSL
                     KNFGTDAMDIAKENVTVNHLKSSIKLTEGEASNPLPYQFDFLEGFSKGYKRVLEKSDSI
                     KKLLNNHSFTSRVIIRPTDQYYLVLDAIIYPENLKSEKNVDNILDYLKPTKLVKDRKIA
                     LDLLKEEKKAIKQGDIPFFYTYNSKHNLKTQGYTTGKAFELTPVDNINVKLNNLSNKRK
                     LLNFQFIAEGFSYIKLKEAEYFNEDNILHFSPLFKNTLKKTTINNPYPIIDLLLDLSIT
                     TNSEIKKTGWFNGMYGNMPSMYNSMTNFSLHDTGGVIFLLEHIKDFRQNPSLVKLDFLN
                     SAKLGLQELQEYFFNDKTSNYFSIISGKSSIDFIFDYKNEKSDNLEKAGFDCCFTKKGN
                     DVFNGTPGLGLVLSSFPLTSSDILFKILSTMVYDGKNHSKKGIAHGELGILWSKYRINK
                     FLKRKDTCKKLFEEVKNITYSLTGWCCGNAGLLMVLAEMCTELNVEKQFLKSIAKKAII
                     LSNNEPIDLSVCHGASGVLQSLLFTYRATNDEWYLSLANTFWQKVLDLSKKNGFYTGEK
                     NRDYILGYFLGWSGFADSCLLLAQFNNKKKPWIPLNLSSSIYQENL"
     CDS             8858..9955
                     /NRPS_PKS="Domain: PKS_ER (47-361). E-value: 9.9e-15.
                     Score: 46.2. Matches aSDomain:
                     nrpspksdomains_BAB78443.1_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1028:crotonyl-CoA reductase / alcohol dehydrogenase
                     (Score: 131.8; E-value: 5.1e-40)"
                     /gene_kind="biosynthetic-additional"
                     /note="unnamed protein product; ORF45; similar to predicted
                     coding region ORF00040 of Lactococcus lactis pMRC01"
                     /protein_id="BAB78443.1"
                     /transl_table=11
                     /translation="MKTLVAISSVKSNSNYHYIGDVNFGTIKIPFGIIEVGKPKFDTSL
                     SENDDYVLLQVKAISCNYRDKAILIENYKKFKKKNISYTPFGSEFCASVISKGSNVSEF
                     EVGETVIPNCFFSDSRENGTGIPTNFASMGWLKIHKNKIITSPKNLTHNEAAGFSLGAQ
                     TATAMIRKINILNTGGTPLVFSARSTTSIFLIKTLLAHNITPICLTTTKWSNKEKRLIY
                     PSIIEKIDKHKYQTLEKIKKKNITHVFDPFFDMNIEDAMYCLDNFGTYITCGLRDQHPL
                     FSSNTPLQHEPKVRNAIRDAIFKNITIIGNCLGKSKDLEDAIKLIEKGSLHVPIDCIYP
                     IDKGINFLTRTFCEKNKLGKCILEL"
     aSDomain        8999..9940
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BAB78443.1_PKS_ER.1"
                     /evalue="9.90E-15"
                     /label="BAB78443.1_PKS_ER.1"
                     /locus_tag="BAB78443.1"
                     /protein_end="361"
                     /protein_start="47"
                     /score="46.2"
                     /tool="antismash"
                     /translation="NDDYVLLQVKAISCNYRDKAILIENYKKFKKKNISYTPFGSEFCA
                     SVISKGSNVSEFEVGETVIPNCFFSDSRENGTGIPTNFASMGWLKIHKNKIITSPKNLT
                     HNEAAGFSLGAQTATAMIRKINILNTGGTPLVFSARSTTSIFLIKTLLAHNITPICLTT
                     TKWSNKEKRLIYPSIIEKIDKHKYQTLEKIKKKNITHVFDPFFDMNIEDAMYCLDNFGT
                     YITCGLRDQHPLFSSNTPLQHEPKVRNAIRDAIFKNITIIGNCLGKSKDLEDAIKLIEK
                     GSLHVPIDCIYPIDKGINFLTRTFCEKNKLGKC"
     CDS             9970..10839
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 155.2; E-value:
                     3.6e-47)"
                     /gene_kind="transport"
                     /note="unnamed protein product; ORF46; similar to ATP
                     binding protein ydbj of Bacillus subtilis"
                     /protein_id="BAB78444.1"
                     /transl_table=11
                     /translation="MILKSISKSYGNEQVLDELSLTLNKNEVVGLIGRNGAGKSTLMKI
                     ITQLDKSFTGSIEGNEDIGYLIEEPKLYNNKTGLEHLKYFSSIYNNNFDLKLYNEFLQN
                     LQMLDVLNRKVKKYSLGMKQKLGILISILNNPSFIILDEPTNSMDIETSSELLKELKKI
                     AQKQNIGILISSHKLEDIENVCDRFLFLKDGKISREETKSSKNTNVISLVFMNEYDLQK
                     FSNTQKFGQIIATDSNTLKLQTKHNINAILQHINDSNIKIRDIFSEKQTLRDIYIKESG
                     GSKIDTKY"
     CDS             10823..11548
                     /codon_start=1
                     /note="unnamed protein product; ORF47; similar to yojE of
                     Bacillus subtilis"
                     /protein_id="BAB78445.1"
                     /transl_table=11
                     /translation="MILNIKKHVMIHFWELLKRGHLLLWLLITILPPLLIAYLSIYKSM
                     PFTIKHVSNFYCMFGMLAAVLHPLYFVNRDYSTKVVSLINNSRRNRLHYVISNYILSMI
                     IALFYASIGIILLVIVKNFGVEGALTLKFIIGFLFNIILLVTTYFALGYLMVLYGVHIG
                     IIYTILTALLLFIHNIFSNILETIQNENAKKFIENLPIYYYPALVGSKPFSFSQYIIGI
                     IIICLITLWIFTKNLRNRY"
     CDS             11577..11750
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /note="unnamed protein product; ORF48; unknown"
                     /protein_id="BAB78446.1"
                     /transl_table=11
                     /translation="MSALNWFILFVISAMLGSFAASILDNIMQSIWIPRICGGLVAALV
                     GGFLYLFFTNEK"
     CDS_motif       11577..11666
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence: MSALNWFILFVISAMLGSFAASILDNIMQS"
                     /locus_tag="BAB78446.1_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       11667..11750
                     /prepeptide="core"
                     /locus_tag="BAB78446.1_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="-6.50"
                     /molecular_weight="2980.6"
                     /monoisotopic_mass="2978.6"
                     /core_sequence="IWIPRICGGLVAALVGGFLYLFFTNEK"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MSALNWFILFVISAMLGSFAASILDNIMQS"
                     /leader_location="[11576:11666](+)"
ORIGIN
        1 atgtactttg atataaatac tttagacatt aaaaatttaa gggatgataa ataccagttt
       61 ttagaagata gtgtcactat caataacgct tattttttag ctgcagatga aattgctatt
      121 aacattttag gggagttatt ttcacttttt tattctgtaa atttggttag attttatatg
      181 tttataatga gtgtcaaacg gaagttcttc cccaacttta ttataaatta aatatcctgc
      241 aatattattt ttatctacat atggtaaatt aacagaagct aaaatataac tattatcatt
      301 ggggtaacaa agataagttt catagagatc ttgattatta tccgccttaa ttataggtgc
      361 ttataaactc tgtgtggttt tcttttgaca tagcagggct ctctcgtaaa tacttttaaa
      421 taatttaaca atcattttca catttttgtt acaaaatttc gaaaaaacag aaaaaacaca
      481 aattcagttg tcaaactatg ttattttatg attataaggt tattcacaat ataggactgg
      541 taactgggaa tatattgaga gtgattttaa ttttataaag cgtggtgatt cttatgaaaa
      601 gttctttttt agaaaaagat atagaagaac aagtgacatg gttcgaggaa gtttcagaac
      661 aagaatttga cgatgatatt tttggagctt gtagtacaaa cactttttct ttgagtgact
      721 attggggtaa taaaggaaat tggtgtactg ctactcacga atgtatgtct tggtgtaaat
      781 aatttattta taaggtggta tttttatgaa aaatgaatta ggtaagtttt tagaagaaaa
      841 cgaattagag ttaggtaaat tttcagaatc agacatgcta gaaattactg atgatgaagt
      901 atatgcagct ggaacacctt tagccttatt gggtggagct gccaccgggg tgataggtta
      961 tatttctaac caaacatgtc caacaactgc ttgtacacgc gcttgctagg aagggagata
     1021 gttcataatg gttttattct acaagaaaga agtatatcca gaactaaata aatcagattt
     1081 taaagaacat attttaccat taaatgattt tattgaagaa ttatctgaac ctttttcaga
     1141 atttatatta gaagatagtg acttaattga gaaggaagaa ttgtatcctt atcataatgc
     1201 ttgtagaata attgctgaag gtgtctggag acatacttgg gatactaagc taaataaata
     1261 tacattaaat cctagcgaat ttaaaaaagc gacttgcgaa ttttattcgc aagtcgcttt
     1321 ttcttatttg ataagaagct ttgctaaaga tataaaatta tttaaattaa gtaatgattc
     1381 taacacagat tatgacattt acacaaaata tttgatagaa aataaatttc aggatttttc
     1441 acaaacgtat aatgtggtgt ggggacgatg tttaaaatta ttaattaata gagcaaaatc
     1501 gatagttaaa accattgaat taataaatca aaaccgtaat gaaattgaaa gaatatttga
     1561 tatacctaat agtcataaga tagtatcaat taatacaagt ggtgatacgc ataataacgg
     1621 ttcttctgtt tcagtagtaa attttgagaa agaattaaag cttatttata agcctagacc
     1681 tatttcagga gaagttggat attcaaaatt aatagaaaag ttgaattgct tagttagttc
     1741 taaattttca tctttaaagg ttttagattt agggggattt ggtttcactt cttatatcga
     1801 aaaaaatgaa atgaacaaaa atatgaaaga agccggtaga attgctgctt taatgtattt
     1861 tttaaatagt tctgatatgc attatagcaa tatttactgg actaataacg gccctattcc
     1921 tattgattta gaaactttat ttcaccctaa aagaatagct gacagtaatt tagaaaattt
     1981 taataatgct tattataaaa ttgagaagtc tgtatatgga acaggaatcc ttcctataca
     2041 tttaactaaa aaatcgagtg atgtttcggt agatgtaggt tttactggag taagagacga
     2101 aaacagcaac tctcccttta aaacttttaa tattgaagat ggtttcactt caaatataaa
     2161 agtagtttgg caaaagcaaa ttgagataga taattctttg tataatgatg aaaattatga
     2221 aaaatttata tatagcaatt gtaaggattt aattgaaggt tttacagaaa tatatatgat
     2281 tattttgaat aataaaaact attttataaa ctgtgtttta gaaacatttg ataattctaa
     2341 attaaggtat atacataaca tgacgtatag gtacgaacaa attttacgaa tacttacgga
     2401 ctccgaacca tcaaaaaata tagatttagc tcatatgttg ctttctcgta ttggaatttt
     2461 aagtatgaca agtaataaaa atattgtttc atcagagtgt acacagcttt ggaatggaga
     2521 tatcccttat ttttctataa gttttgatca aaaagcagta gaatcagaaa ataaatttgt
     2581 cacttggtta cctaaatctc ccaaagaaga atttatgata aaaatgaatt cgatttcttt
     2641 agaagactta aatagacaaa ttgagataat aaaattagcg tttgttgcta aattagcaga
     2701 tcctcataga gacgaacaaa ttttagaaag tgaaattaat gaggacgttt atatagataa
     2761 aaaaactata gagcattcta tctcatattt tatgaattca ttgactgaaa gaataatgaa
     2821 tgaccggtat aaccatttgc ctaaaacttg gataggacct gtctctcaat atggtaaggg
     2881 atggactcca ggagttttag gctatgattt atatagtggc agaactggcc cctttctagc
     2941 tttattatta tctggaaaac aattaaaaaa ccaaaaggta atagatgttg cttatgagtt
     3001 ttttgataaa gttactaaaa tatttgagga gaaaacatac gacttaagaa atttacttat
     3061 gtctgggata ggagcttttt ctggaatgtc aggcattata tggagccttt atactgctgg
     3121 aaagatcact gaaaattcta aatggaaaaa tacagcttta aaagtctttg atttaattga
     3181 tgaagaaata aagaaagaag agaaaggcga cttttttgat atgatttcag ggagtagcgg
     3241 agcaattatt atgagatata aaatccaaga agattactcg ttatctgaaa atttcataaa
     3301 agaaaaggtt aaagaagctt ataatattat aaataataag aaaaatctat cctctggatt
     3361 agctcatgga ataagccaga ttatgtattt ttttgcaatt atttataaaa aacaaaagct
     3421 agtatatata agaagaataa tagaagatat tcataaaatt atcaaatcaa attttactaa
     3481 tgtgcaaaat ttaattgaaa tttataggtc ggaagacaaa aataatattt catcgtcttg
     3541 gtgcaatggt ttatccggac tactagtttc atattacgaa ggatataaag ctggaatttt
     3601 ttcaaaagat gaagttatag atataattaa gcaaattgaa attatacctt tatctgtagt
     3661 tccgatactc tgtcatggta gtttaggtgt ttatgattct ttgaaatatg taagcaaaga
     3721 ctttgaaaaa gaaacacgtt atttattaat gaaattagaa aaaactactt gtagtcctgc
     3781 gtatatagta aattacttta aagaaggaag aggtagatat acacttagcc caggactaat
     3841 gacaggtaaa accggagctc tattacattt atgtaaattg ctagatggtg aaattgatgt
     3901 ctctccccta acttttgagg tgtaatatgg ctaaattgaa aatatttaat aaaaaaagag
     3961 ttccaccatc tttacaaatt gctcaaacag aatgcggatt gtgttgtgtg aaaagtatac
     4021 tgaattatta tgattttagt gtttctttac cagaacttag aaaaattaaa gaaccaggaa
     4081 gagatggttt agggttaaaa cagattaaag gtttgttgga atgttataat ttcaaagtga
     4141 agaattttaa ggttaaagat aataaagctt tcgatatgtt gaattttccg ttaatagcct
     4201 tttggaaagg ctgtcattac gttgttttag agtcttataa tgataaaaac gtagttatta
     4261 tggatccatc agtagggaga ataaaaataa gtactttaga atttaatgct aattttgata
     4321 gatacgtgat tttatgtact ccaaatgata attttcaaaa acgtaatatt tcaacggtat
     4381 ctaaatttaa aaaaacatat atttggccaa acaatatgat aagtatatat gtaaaattag
     4441 cacttttgag tttgatagca attactatta cattgtcaat tcccatagcc acacaaaaat
     4501 taatagactt tagtctacaa actcaaaaat ctttaatttt tatagttatt atcattattt
     4561 tctcaatgat aatagcagtg ttcttgtcat ttttgagaaa cttgtattct atacaaatga
     4621 ttttaaaatt taattggttt ttaatgaatg gtgcttttaa acgtttatta tatttgcctg
     4681 caaaatactt cagcgttcgt tctccaggag aaattacgta tagaattaat gcattaactc
     4741 gcattcaaga tattataggc cctacattgg tacgttctat cttagatttt tttagtagtt
     4801 gcactttatt aggttatgtt ttttttatta atccactaat tgcaatatta attttaatct
     4861 ttttgttatt gaattttatc tttttagcta ttatgcaaaa acacattaac ttttcaaagg
     4921 acagagagtt acatgaagga agtagtgctc aaagtatcca actagatgct ttagtatcta
     4981 taaatagtgt taagataggt ggatacatga aacgttacat agatgattgg gctaacaggt
     5041 atcaaaattt actgaaagct atggaaaaaa gaatgaaatt acaagatggt ttggttggtt
     5101 caatattgaa cggctttcaa acttttgctc cactgctaat tttatctatt aatatttatt
     5161 tatctcaagt ggggtatatg actttaggac aggcgattgc agtacagtct gttgtttcta
     5221 tgttgttcac atatactaat tctatattta ctactatttc tgaagctttg acggcaacga
     5281 aatatattga attatcagaa gatatttttg aataccctga ggagaatcgt attaatagca
     5341 tgattcaaat gcagtcagga gatgtagaaa ttaagaattt gcactttagt tacacaccag
     5401 aaagcccttt agcaataaaa tctattaatt taagtataaa agagggtgac actatagctt
     5461 tagtgggcat gtcgggatca ggtaaaacta cattaggtaa acttattagt actcttttcg
     5521 aaccttctag tggcagcatt ttatatgggg gtgtagagaa aacaaaatat aacattgata
     5581 atttaagatc atctattagt tacataccac aagaagcata ccttcataat agaacaattt
     5641 tggaaaattt gaagttgggt tgtaactaca atgaatacga aataatcaaa cgttgccaat
     5701 ctatgccatt tttaaacttt attaatagct tgcctatggg atatagtaca gtagtttctg
     5761 aaatgggtgc taatttgtca ggaggtcaac gacaaagaat acatatagca aaagttcttc
     5821 tgcaatctcc taaattatta gttatggatg aagctacttc ttcacttgat aatgtgtcgc
     5881 agtcacatgt atatgacgaa ctatcgagat taaaatgcac caaaatcgta attgctcatc
     5941 gatttgctac tgtatttaat gcagataaaa tagttgttat ggatcatgga gaaattgttg
     6001 aagttggcgc acataatgaa ttgttaaata aaaatggttt atatagaaaa ctatttaatg
     6061 ctgaatttaa aaaggagtgt ggagtttgaa taagataaaa gatgtaacaa atataatcac
     6121 tcgagctatt gaagaaataa atattaatat gttggaaaat aataatatac aaattaaaaa
     6181 aataacagaa gacatagacg atggagataa tataaataat ttaattgaac aatgcagaca
     6241 atcgttaatt gaaatcaatg tctggaaaat cacgtataaa acttttgttt atgaatttca
     6301 tgagtttaga aaaaaaatta attatcctat tagcactaac tcgtctatag cgtataatca
     6361 ttatattaat aatatatctg cagaaactct aatacattgg tttaataaat atccatattt
     6421 aagagagata attgaaaaaa ttattaataa tatctgtgag ttttcattaa aagtctgtca
     6481 tgatttaata acggattgga atattcttat agaaaaaaac cttataaata aaggtgaaaa
     6541 attgcaaaat attacttgct tggattcaga tccacataat aataataaag ttggactatg
     6601 ttttgatttt gaaaagtcaa aagttttata taaacctaaa tctttaaaag tagatatgct
     6661 tatttctgaa atatttgaaa atattttaca gtttgaagag ctgaacaaaa ttatacctgt
     6721 tgctacaagt atagatagag gagattatgg atggcaacgt tttattaatc gaaattatct
     6781 caataaagat gatcttccta aagcatttta caatttaggt ctatgttcgt ctttatttac
     6841 tgctctaggt gctacagatt tgcatgatga gaatataatt ttcaataacg aatatccata
     6901 ttttatcgat ttggaaacat gtcttcagcc tgaaattaaa tatataaata attcattagt
     6961 agatactatg ctggataata ttaaatcttc tatagctagt acatctataa ttccttctaa
     7021 tataataagt tatttacagc agatattagt aggtgcaatt aatactccct accctcaaaa
     7081 aactaataaa aggcgttttt ctttaaaaaa ttttggcact gatgctatgg atatagccaa
     7141 agaaaatgta accgtgaacc atttaaaatc ttctataaaa ctaacagaag gtgaagcaag
     7201 caacccttta ccctatcaat tcgacttttt agaaggtttt tcaaaaggtt ataaacgggt
     7261 cttggaaaaa tctgactcaa taaaaaaact tttaaataat cattctttta caagtagagt
     7321 aataataagg cctaccgatc aatattactt agttcttgat gcaattatat acccagaaaa
     7381 tctaaaaagt gaaaaaaatg tcgataatat tttagactac ttaaagccaa ctaaattagt
     7441 taaagataga aaaattgctc tggatttatt aaaagaagaa aaaaaagcta taaaacaagg
     7501 agatatcccc tttttttata cttataattc taagcataat ttaaaaacac aaggttatac
     7561 gacaggaaaa gcttttgagt taactccagt agataatatt aatgtgaaat taaacaattt
     7621 atcaaataaa agaaagctat taaacttcca atttatagca gaaggttttt catacatcaa
     7681 actaaaagag gctgagtatt ttaacgaaga taatatatta cacttttctc cgttatttaa
     7741 aaatacactt aaaaagacaa ctatcaataa tccatatccc attatagatt tgttattaga
     7801 tttaagtata actactaatt ctgaaataaa gaaaactggt tggtttaatg gaatgtatgg
     7861 aaacatgcct agtatgtata attctatgac taacttttct ttacatgata cgggaggtgt
     7921 gatttttctc ttagaacata ttaaagattt taggcaaaat cctagtttag ttaagttgga
     7981 ttttctaaac tcagctaaac taggattaca agaactacaa gaatattttt ttaatgataa
     8041 aacaagtaat tatttctcga ttatatctgg aaaatcatct atagatttca tttttgatta
     8101 caaaaacgaa aaatctgaca atttagaaaa agctggcttc gactgttgtt ttacaaaaaa
     8161 aggtaatgat gtttttaatg gtactccagg attaggatta gttctatcct cttttccact
     8221 tacttcgtca gatatattat ttaaaattct aagtacaatg gtttatgacg gtaaaaatca
     8281 cagtaaaaaa ggaatagctc acggtgaatt aggtatttta tggtctaaat atcgtattaa
     8341 taaatttttg aaaagaaaag atacatgtaa aaaattgttt gaggaagtta aaaatattac
     8401 ctattcttta actgggtggt gttgtggcaa tgcagggtta ctcatggttt tagcagaaat
     8461 gtgtacagaa ttaaacgtgg aaaaacaatt tttaaaatct attgctaaaa aggctataat
     8521 actttctaat aatgaaccaa tagatttatc agtgtgtcat ggtgcaagtg gtgttttgca
     8581 atctttacta ttcacttata gagcaacaaa tgatgagtgg tatttatctt tagctaatac
     8641 cttttggcaa aaagttttgg atttatcaaa aaagaatggt ttttatactg gcgagaaaaa
     8701 tagagattat atactagggt actttttagg gtggagtggt tttgcagata gttgtttatt
     8761 actcgcccaa tttaataata agaaaaaacc ttggatacca ttgaatttaa gctctagtat
     8821 ataccaagaa aatttatgat aggagcgtaa atatttgatg aaaactttgg ttgcaataag
     8881 ttcagtaaaa agtaattcta actatcatta cataggggac gtgaactttg gtacaatcaa
     8941 aatacctttt ggaattatag aagtcggaaa accaaaattc gatacatctc tttctgaaaa
     9001 tgacgattat gttttgttac aagtcaaagc aatttcttgt aattatagag acaaggcgat
     9061 tttaatagaa aattataaga aatttaaaaa aaagaatatt tcatacacgc cttttggttc
     9121 tgaattttgt gccagtgtta ttagtaaagg gtctaatgtt tctgaatttg aagttggaga
     9181 aacagtaatt cctaattgtt ttttttctga cagtagagaa aatggaactg ggatacccac
     9241 taattttgct tctatgggat ggttgaaaat tcataaaaat aaaataataa cttcgcctaa
     9301 aaatttaact cataatgaag ctgctggttt ttctctaggt gcacaaacag ctacagcaat
     9361 gatacgtaaa attaatattt taaatacagg tggaacacct ttagtgttta gtgcgcgctc
     9421 tacgacatca atttttttaa ttaaaactct tttagcacat aatattactc ctatatgttt
     9481 aactacgaca aaatggagta ataaagaaaa aagacttatt tatccaagta tcatagaaaa
     9541 aattgataaa cataagtatc aaacattaga aaagataaag aagaaaaata taactcatgt
     9601 tttcgatccc ttttttgaca tgaatataga agatgctatg tattgtttgg ataactttgg
     9661 tacatacatt acatgtggac ttagggatca acatcctctt ttttctagta atacaccatt
     9721 acaacacgaa cctaaagtta gaaatgctat tagagatgct atttttaaaa atatcacaat
     9781 cattgggaat tgtttaggaa aatccaaaga tttggaagat gctattaaat taattgaaaa
     9841 aggttcttta catgttccta ttgattgtat atatccaatt gataagggaa taaatttttt
     9901 aacacgaact ttttgtgaaa aaaacaagtt aggaaaatgt attttagaac tataaaaagg
     9961 agaattctga tgattttaaa atcaattagt aaatcttatg gtaacgagca agtattagat
    10021 gaattatctt taacattaaa taaaaatgaa gttgtaggtt tgataggaag aaatggagca
    10081 ggtaaaagta ctctaatgaa aataatcact caactcgata aaagctttac tggtagtatt
    10141 gaaggtaacg aagatattgg ttatttaata gaagaaccca aattatataa taacaaaact
    10201 ggactagagc atctaaagta tttttcttca atttataaca acaactttga tttaaaactg
    10261 tacaatgaat ttttacaaaa cttacaaatg ttagatgtat taaatagaaa agtaaaaaaa
    10321 tattcgctag gtatgaaaca aaagctagga atacttatta gcatattaaa taacccgtct
    10381 tttattattt tagacgagcc cacaaatagt atggatattg aaacttcgtc tgaattatta
    10441 aaagaattaa aaaaaatagc tcaaaaacaa aatattggta tacttatatc aagtcataaa
    10501 ttagaagata ttgaaaatgt ttgtgataga tttttatttt taaaagatgg taaaatttct
    10561 agagaagaaa ctaaaagctc aaaaaatact aacgttatta gcttggtttt tatgaatgaa
    10621 tatgatttgc aaaaattttc aaacacgcaa aagtttggtc aaataatagc tactgactct
    10681 aatacattaa agttacaaac taagcacaac attaatgcga ttttacaaca cataaatgat
    10741 tccaacataa aaataagaga tatattttca gaaaaacaaa ctttacgaga tatctatatc
    10801 aaagaaagtg gaggttctaa aattgatact aaatattaaa aaacacgtaa tgatacactt
    10861 ttgggaatta ttaaaaagag gacatctatt attatggtta ttaattacta tactaccccc
    10921 tttgttaata gcgtatcttt ccatatataa aagtatgcca tttactatta aacatgtatc
    10981 aaacttttat tgtatgtttg gcatgcttgc cgcagtctta catccactct attttgttaa
    11041 tagagactat tctactaaag ttgtatcctt aattaataat tctagaagaa atagacttca
    11101 ttacgtaata tctaattata tattatctat gataatcgct cttttttatg cttctatagg
    11161 cataatacta ttggttatag ttaaaaattt tggtgtagaa ggtgcattaa ctctcaaatt
    11221 tattataggg tttctattta atataatact attagtcact acatattttg ctttaggata
    11281 tttaatggtt ttgtatgggg tgcatatcgg tattatatat actattttaa cagcattatt
    11341 attattcatt cacaatattt tctcaaatat attagaaacc atacaaaatg aaaatgcaaa
    11401 aaaatttata gaaaatttac caatatatta ttatcctgca ttagttggtt caaaaccttt
    11461 ttctttttca cagtatatta taggtataat aatcatatgt ttaataacat tatggatatt
    11521 cactaaaaat ttgagaaatc gttattaaaa acaatatttt atatggagga attttaatga
    11581 gtgcacttaa ttggtttata ttatttgtta ttagcgctat gttaggttct tttgcagcca
    11641 gtattttaga taatattatg caaagcattt ggatcccaag aatttgtgga ggattggtag
    11701 ccgctttagt aggtggcttt ttatatcttt tttttacaaa tgaaaagtga
//
