LOCUS       BGC0000556             12040 bp    DNA     linear   BCT 08-MAY-2003
DEFINITION  Streptococcus pyogenes genes for SrtI, SrtR, SrtK, SrtA, SrtT, SrtC,
            SrtB, SrtF, SrtE, SrtG, complete cds.
ACCESSION   BGC0000556
VERSION     BGC0000556
KEYWORDS    .
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 13:50:18
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..12040
                     /db_xref="taxon:1314"
                     /mol_type="genomic DNA"
                     /organism="Streptococcus pyogenes"
                     /strain="BL-T"
     protocluster    1..12040
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[4931:9161]"
                     /cutoff="20000"
                     /detection_rule="((LANC_like and (Lant_dehydr_N or
                     Lant_dehydr_C)) and not (YcaO or TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      4932..9161
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="((LANC_like and (Lant_dehydr_N or
                     Lant_dehydr_C)) and not (YcaO or TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
     cand_cluster    1..12040
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((LANC_like and (Lant_dehydr_N or
                     Lant_dehydr_C)) and not (YcaO or TIGR03604))"
                     /kind="single"
                     /product="lanthipeptide-class-i"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..12040
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000556"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..12040
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lanthipeptide-class-i"
                     /region_number="1"
                     /rules="((LANC_like and (Lant_dehydr_N or Lant_dehydr_C))
                     and not (YcaO or TIGR03604))"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            253..570
                     /gene="srtI"
     CDS             253..570
                     /codon_start=1
                     /gene="srtI"
                     /product="SrtI"
                     /protein_id="BAB08159.1"
                     /transl_table=11
                     /translation="MAQLDLTITELQDHIAHLNKVAEVLLNLNNNDIENRRLARYDYAK
                     MNLTAAIKIEEVEKEIETSQNELNISIDEYEYLVRRLEKFGEILSDSKIIDTSRNEIQW
                     E"
     gene            721..1407
                     /gene="srtR"
     CDS             721..1407
                     /codon_start=1
                     /gene="srtR"
                     /gene_functions="regulatory (smcogs) SMCOG1008:response
                     regulator (Score: 162.8; E-value: 1.5e-49)"
                     /gene_kind="regulatory"
                     /product="SrtR"
                     /protein_id="BAB08160.1"
                     /transl_table=11
                     /translation="MYKILAIDDDKEILKLMKTALEIENYHVITCQEIELPIVFDDFKG
                     YDLILLDIMMPNISGTEFCYKIREEVHSPIIFVSALDGDNEIVQALNIGGDDFIVKPFS
                     LKQFVAKVNSHLKREERAKIKNEAEERVKRSFPPIEIYLEERMLYIDKQPLFLTYREYE
                     ILELLSRHPYKVFTKEEIYEQVYSDEASALFHSISEYIYQIRMKFSSFGINPIKTIRGI
                     GYKWDV"
     gene            1400..2746
                     /gene="srtK"
     CDS             1400..2746
                     /codon_start=1
                     /gene="srtK"
                     /gene_functions="regulatory (smcogs) SMCOG1003:sensor
                     histidine kinase (Score: 94.2; E-value: 1.7e-28)"
                     /gene_kind="regulatory"
                     /product="SrtK"
                     /protein_id="BAB08161.1"
                     /transl_table=11
                     /translation="MFKKYSIKNRVWRAVVEIVFGVCITVLMIAIISLSFSKLNIVTSH
                     NVGEEFYIKDKQSIKQLNNYMKTLGLDYVVFDRKTDKAMEGKYLSKEFSLFNEVAEEKN
                     NLTFNSVHYDLYTNINYNIVIRYNEIPEFSNHYLRNVSYNMLTFYILGIGTSISIVVAL
                     TRFVKEISLNFKEIKKLANKMGIEVLSENENYSKIIEFDDILRTLHIKGDNLKSLIERE
                     ILEKQDLSFQIAALSHDIKTPLTVLKGNLELLELTTLNKNQEGYIVSMNNSISVFEGYF
                     NSLISYTRMLSEDRSVKLILVEELLSELHFEVDDLLNINNIEFSICNRLIITSFYGDEE
                     NLIRALSNLLVNAIRFMPVLDKKIEVILSESGEQIHFEIWNNGERFSDSTLKKGDKLFY
                     TEDYSRGNKHYGIGLAFVKGVAIKHGGNLQLNNPARGGASAIISIKKKI"
     gene            2903..3043
                     /gene="srtA"
     CDS             2903..3043
                     /codon_start=1
                     /gene="srtA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR03731"
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /note="latibiotics precursor"
                     /product="SrtA"
                     /protein_id="BAB08162.1"
                     /sec_met_domain="TIGR03731 (E-value: 4.7e-11, bitscore:
                     32.1, seeds: 23, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNTIKDFDLDLKTNKKDTATPYVGSRYLCTPGSCWKLVCFTTTV
                     K"
     CDS_motif       2903..2971
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence: MNNTIKDFDLDLKTNKKDTATPY"
                     /locus_tag="srtA_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       2972..3043
                     /prepeptide="core"
                     /locus_tag="srtA_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class I"
                     /score="16.50"
                     /molecular_weight="2441.9"
                     /monoisotopic_mass="2440.3"
                     /core_sequence="VGSRYLCTPGSCWKLVCFTTTVK"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MNNTIKDFDLDLKTNKKDTATPY"
                     /leader_location="[2902:2971](+)"
                     /alternative_weights="2459.9"
                     /alternative_weights="2477.9"
                     /alternative_weights="2496.0"
     gene            3158..4948
                     /gene="srtT"
     CDS             3158..4948
                     /codon_start=1
                     /gene="srtT"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 156; E-value:
                     2.1e-47)"
                     /gene_kind="transport"
                     /product="SrtT"
                     /protein_id="BAB08163.1"
                     /transl_table=11
                     /translation="MIKAQLKNLIKTIHLVKEAGFSLLVFSIAISLFQGILPIFSMLLV
                     QKMLNIITTDIKNFHTLMIAFISYIALTLLTIIIGEVDSYIDTKLQILLHYKMNHLVMQ
                     KTVKLTLAEFETPEIYDDITRIQNQISYKPFQIYKSIISVLSSLVSLISSFVILLNWKI
                     SIFPLLLILPIVSIYIYLKIGKNEFEILYKRSSDERANWYISHLLTHDFAVKELRLGTL
                     ESYFLKKYDKNNQLFINQENSINRSKMKYGIVLGVLEIAIQSIIMFLAVREAFLGILLV
                     GNVTTFIRSLSTMQTSTQNIVNNIYSFYNGSLYMELLYDFTHDELVHNPIIESTVDTIQ
                     SVEFERVSFSYNGKDNVINDISFSIKAGERLAIVGENGSGKSTIFKLACGLYDNYEGNI
                     YINGINLRSIQKKSYYKRISALFQDFLKYELTLRENVGLGELSKLYSDEDLISALKYTG
                     VDSIFYFNQEKLNQIDLEQQLGNWFEDGRQLSGGQWQKIALSRVYLKEADCYLLDEPSS
                     ALDPESETKIFKTFFELSQNKIGIFITHKPSITQSVNKILYLDNGCISETGSFADLNIK
                     GMKFKNLLDKEIGATYDTSN"
     gene            4932..6176
                     /gene="srtC"
     CDS             4932..6176
                     /codon_start=1
                     /gene="srtC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: LANC_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1140:Lanthionine synthetase C family protein (Score:
                     294.4; E-value: 2.2e-89)"
                     /gene_kind="biosynthetic"
                     /product="SrtC"
                     /protein_id="BAB08164.1"
                     /sec_met_domain="LANC_like (E-value: 4.1e-80, bitscore:
                     258.5, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTPQIKQIVDYIADSFINYEQIVAESENKVLINIESKYRNTFFDK
                     SLSHGVPSLILMYSSLYKVTKDERYLTFSNIYVEKLVDIISRDGIESPSLYAGTAGIAL
                     AIKEASISGKYYPKLLSSLNCLLKEQIKDKLVVSFSNIEKGIIEPYDYDMVNGFSGITN
                     YLLLEQEFFSVELNQIGNYLLKYIETILNKVTNCSEDREAEFDLGIAHGITGPMLILAK
                     LKSEKILSVEVGDILNKAINLMFLFRRDDKLWPGKIYTSNILDLDLNYLPTRMAWCYGT
                     PGTAVALFKMCQLIDLNFIDDINESLIAQMRSSDKNVYSLTMCHGLAGVTFIYDLIGKN
                     YSSERLTRFAEELRQQIITSFSTTKVFGYTDREKFGEIEVELESIGILQGVSGIVLFLL
                     NPYSDEELLWQRIFI"
     gene            6195..9161
                     /gene="srtB"
     CDS             6195..9161
                     /codon_start=1
                     /gene="srtB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lanthipeptide_LanB_RRE"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1155:Lantibiotic dehydratase domain protein (Score:
                     827.1; E-value: 3e-250)"
                     /gene_kind="biosynthetic"
                     /product="SrtB"
                     /protein_id="BAB08165.1"
                     /sec_met_domain="Lant_dehydr_N (E-value: 1.2e-92, bitscore:
                     300.4, seeds: 73, tool: rule-based-clusters)"
                     /sec_met_domain="Lanthipeptide_LanB_RRE (E-value: 9.2e-14,
                     bitscore: 41.5, seeds: 20, tool: rule-based-clusters)"
                     /sec_met_domain="Lant_dehydr_C (E-value: 1e-15, bitscore:
                     47.5, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYKVFPTGMIRIPLQKQEFYNKDYEDLINDKVFMEQLLVASPSLY
                     DSVIDFDKCNEKKKQNVKVSLNSYSKRAAFRTTPFGIFTAINTVDLTKGTTSNVQKVSF
                     IKKAVPDYSWIYSLVKSYEISNLEKLSLKINTAAFTQGDRYVLPFTVNESEEDRNISFS
                     KPIKLLVEKCKTRYIKYEELIDVFKNNYPDIVSDMLESYIHDLVTNDFLISDLRPPICN
                     INSLDYLLSKLEEGTLCTDLTTLKKMIEDYNDLNIGEGRDLLLQIFDKMESIYKYDNGG
                     NYLKVDSLAEYEGDFNILSETARESIERLAEMFVKLNTNVKRRGGRKNSLEEYQLKFIG
                     KYGENCSIPFVEVINKDAGIGFPEYYKGGEGEAIELSDPIMQMFEKKYEEALLNGGHIE
                     FYSKDLDDFQTDQSNSLDSFELNFNIKIINNDVKLYLGANIGSGQAGRSFGRFYGLSET
                     VRETIKNLNHQNNTNVELSFVPKQIRLANVIQNYSDESYNTSFFTTSWDSENELRLEDI
                     YIRYSDGKFHFTTIDGKNELKFTMNNMLNSDSQSRVLRLLVDLSEFEYGLSHWSLFPWD
                     ILAQERVYIPEILFEDITIATAQWNLSVIREEIIHQKIFTGKKDIFNKFRKTYKIPKDI
                     ILKYADNELRLDLSIDSDLEILFKNINKYRFVSLRGIEQGESIFKDNLGNYNTEIVVPI
                     LKESNGINNTNDNVQFKNEVKKIYLPFCDWLFFKFYGPKERQEELLNNWKKFWLNLPSE
                     LFSQSNMFYMRYNDTNDHIRIRINCDSIENNFSLYLSVVQDLLPQLIESCIISDIEVSS
                     YKPEVNRYGGPHLISYAEEIFCKESILFLNNTIEFSDDERLVCATYLVLYYLNHFFKDK
                     ETRRSFLFENYTGKYKKDFKNLPIDLPIEYMKILNGVTSALDRYDFFQEMDRYLMSYMK
                     EYNRFGGTNDIYNTKFNLVGSFLHLSMNRLNGIDREFEEKVYCFAYYTLNAQQYIEWE"
     gene            9167..9856
                     /gene="srtF"
     CDS             9167..9856
                     /codon_start=1
                     /gene="srtF"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 177.8; E-value:
                     4.5e-54)"
                     /gene_kind="transport"
                     /product="SrtF"
                     /protein_id="BAB08166.1"
                     /transl_table=11
                     /translation="MLKIQNLKKSYGKRTILNNVNMNIPKGKVYALIGPNGAGKSTIMK
                     ILTGLVSKTSGSIIFEGREWSRRDLRKIGSIIEEPPLYKNLSAYDNMKVVTTMLGVSES
                     TILPLLNKVGLGNIDKRPVKQFSLGMKQRLGIAISLINSPKLLILDEPTNGLDPIGIQE
                     LREIIESFKSEGMTIMISSHILSEVEHLADFIGFIYEGKIILEKEYDGSENLEELFNNQ
                     ILFEKRR"
     gene            9862..10611
                     /gene="srtE"
     CDS             9862..10611
                     /codon_start=1
                     /gene="srtE"
                     /product="SrtE"
                     /protein_id="BAB08167.1"
                     /transl_table=11
                     /translation="MVQIFLSNLLKIKNTSISKLILIFPIICVMLSLLFSALGGENILR
                     LTAETTVNQWGVIWINVIIAVNSGLLNKLELDSNKYSVFLSRDVDLKKVEYARVLLIAL
                     INLIISMILSLMLIVISFVLPTPSLISIGRILLTILLIWLTTLWQIPFILWLSRKINVY
                     FAMIINIISPLIIGTSFSLLNKWYLFPYDWSLKLLEPMTRMRINSIPFGAEFVPDYSQI
                     FISLFLGIAFFILLTNLFAISFKKQVK"
     gene            10614..11336
                     /gene="srtG"
     CDS             10614..11336
                     /codon_start=1
                     /gene="srtG"
                     /product="SrtG"
                     /protein_id="BAB08168.1"
                     /transl_table=11
                     /translation="MKSYLCSNFLKIKNTSYLLIHILAALLFPLLLFFYWSQRGGLQSR
                     TVLFSYFQIVGVLLPFVASIVCIQLKNLEESSGKYKYLLGYSQSNYKPFIVELVFLWLC
                     YCIVLIISITIFILLLKTIGINVSLRLLILNSLIYIIFAYVTYLINHIISYIFSTGVAL
                     GISMVGVIAAAFCETSLGDKVWFLIPWAWLLRISDTLYNQQKMAIVPLIVIFFVSCTVA
                     LLHIRIFKSWNQDCLTSS"
ORIGIN
        1 aaaatataaa gtctatattc gtgagaccgc acaattcttc atttacaaca aggaggcgtc
       61 taaacttaat cgctatgtgc gagggcgtga gctcatttac cagttaacca atgatagcaa
      121 gagtattcca aaaagaagac gaccaaaacc attgatacac ttaagaaaaa gattgaagaa
      181 ataagtctcc tcattgagtt agatacggag aataagcctt atcaagatat taaagatgac
      241 attgttaaag atatggctca attagatttg accattacag agttgcagga ccatattgct
      301 catctcaata aggttgcgga ggtcttgctt aatctgaaca acaacgatat agagaaccgc
      361 cgtttggcca gatatgacta tgccaagatg aacttgactg cagctataaa aatagaagaa
      421 gttgagaaag agattgaaac ttctcaaaat gaacttaata tatccataga tgagtatgaa
      481 tatctagtaa gaaggttgga aaagtttgga gagatcttga gtgatagcaa aattatcgat
      541 acttctcgaa atgaaataca atgggagtaa ttatgatata atttgggtgg tagcaatttt
      601 tatatcagat attaaaatct agttgatgtt tcgtaaataa ctatattaaa gaaatgattc
      661 ctgccgctta cattaagaat agtttatttg taagtatttc attatttaaa aggggtaata
      721 atgtataaga ttttagccat agatgatgat aaagaaattc tgaagcttat gaagactgct
      781 ttggagattg aaaattatca tgttataacc tgtcaagaga tagaattacc aatagttttt
      841 gatgatttta aaggatatga tttgatctta ttggatatca tgatgcctaa tataagtgga
      901 actgagtttt gttataaaat tcgggaagaa gttcattctc caattatttt tgttagcgct
      961 ttagatggcg ataatgaaat tgtccaagct ttaaatatag ggggagacga ttttattgtg
     1021 aaaccattta gcttaaaaca attcgtagct aaagttaact ctcatttgaa gagagaagag
     1081 agagcaaaga taaagaatga ggctgaggag agagtgaagc gcagttttcc acctatagaa
     1141 atctatctag aggaacgtat gttatatatt gataaacagc cgttattcct gacttataga
     1201 gagtacgaaa ttttagaatt actgtcacgt catccgtata aagttttcac aaaagaagag
     1261 atatatgaac aagtatatag cgatgaagct tcagcattgt ttcattctat ttcagaatat
     1321 atctatcaga ttagaatgaa attttcaagt tttggaatta atccaataaa aactattcgc
     1381 gggattggtt ataaatggga tgtttaaaaa atattcaata aaaaatcgtg tttggcgcgc
     1441 cgtcgttgaa attgtctttg gagtttgtat cactgtttta atgattgcga taatatcgct
     1501 ttctttttca aagttaaata tagtgacatc tcataatgtg ggagaagaat tttatattaa
     1561 agataaacag tcaatcaaac agttgaacaa ttatatgaag acattgggat tagattatgt
     1621 tgtttttgat agaaaaacag ataaagctat ggaaggaaaa tatttatcta aagaattttc
     1681 tttatttaac gaagttgcag aagaaaaaaa taatctgact tttaattctg tacattatga
     1741 tttatatact aatatcaatt ataatattgt cataagatat aatgagatac cggagttttc
     1801 taaccattac cttagaaatg tttcatataa catgttgaca ttttatattc tgggaatagg
     1861 aacgagtatc agcattgtag ttgctttaac acggtttgta aaagaaattt ctttgaattt
     1921 caaggaaatc aagaagttag caaataaaat ggggatagaa gtcctttctg aaaatgaaaa
     1981 ttattctaaa ataatcgagt ttgatgatat acttagaact ttacatatta aaggtgataa
     2041 tttgaaaagt ttgattgagc gagaaatatt ggaaaagcaa gatctatcct ttcaaattgc
     2101 agcactttcg catgatataa aaacaccttt aacggttttg aaaggaaatc ttgagttatt
     2161 agaattgact actttaaata aaaatcagga aggttatatt gtatctatga acaatagtat
     2221 ttctgttttt gaaggctatt tcaattcttt aatatcttat acacgcatgc tttcagagga
     2281 tagatcagtg aaactaattt tagttgagga gctattaagt gagttacatt ttgaagtcga
     2341 tgacttattg aatatcaata atattgaatt ttctatttgt aatagactta ttatcacttc
     2401 tttttatgga gatgaggaaa atctgataag agctctttct aaccttttag ttaatgcaat
     2461 aagatttatg cccgtattag ataaaaaaat cgaagttata ctgtctgagt caggagagca
     2521 aattcatttt gaaatatgga ataatggtga gcgtttcagt gatagtacac tgaaaaaggg
     2581 agataaatta ttttacacgg aagattatag cagaggtaac aaacattatg gaatcggttt
     2641 ggcatttgtt aaaggcgttg ctatcaaaca cggtgggaat cttcaattga ataatccagc
     2701 tagaggaggt gctagcgcca taatctcgat aaaaaagaaa atttaataga tttgattgaa
     2761 actggagtat ttcaggcaaa aaatcgtaaa ttttggtgat ttttggctaa aatacttttt
     2821 ttctgaacaa attctgaact tggggtgtca taataatcat gtaaatatat cggatgcaaa
     2881 aacgtaagag aggaactaga aaatgaataa cacaattaaa gactttgatc tcgatttgaa
     2941 aacaaataaa aaagacactg ctacacctta tgttggtagc cgttacctat gtacccctgg
     3001 ttcttgttgg aaattagttt gctttacaac aactgttaaa taataaagtt atcattatca
     3061 tcaacattat aatttcaaat aatttgatat atttataaat ttaggggagt agactgattt
     3121 tagtttgctc ctttattagt agataaggaa gttatacatg attaaagctc agctaaagaa
     3181 tctaataaaa acaattcatc tcgtaaaaga ggctggtttc tccctactgg tgttctcaat
     3241 tgctattagc ttatttcaag ggattttacc catttttagt atgctactag ttcaaaaaat
     3301 gttaaacatt ataacgactg atataaaaaa ttttcacaca ttgatgattg cttttatctc
     3361 atatattgct ttaacattat tgacaattat aataggagaa gttgatagtt atattgatac
     3421 taaattacag atacttcttc actataagat gaaccattta gttatgcaga aaactgtaaa
     3481 gttaacattg gctgaatttg agacaccaga aatctatgat gatatcactc gcatacaaaa
     3541 tcaaatatct tataaacctt ttcaaatata taagtcaatt atttctgtat tatcttcgtt
     3601 agtatcatta atttcctcat ttgttatttt attaaattgg aagatatcaa tttttccact
     3661 tttacttatc cttcctatcg tttctatata tatctacttg aaaattggta aaaatgaatt
     3721 tgagatacta tataaaagga gtagcgatga gagagcaaat tggtatatta gtcatctttt
     3781 aacacatgat tttgctgtca aggagttgag gttaggtacg ttagaaagtt attttctaaa
     3841 aaaatatgat aaaaataatc aattatttat taatcaggaa aatagtataa atagatctaa
     3901 aatgaaatac ggtatagtat taggtgtatt ggagattgct atacaatcta ttattatgtt
     3961 ccttgcagtt agagaagcat ttctaggaat attattagtt ggaaatgtaa ctacttttat
     4021 aaggtcttta tctacaatgc aaacaagtac acaaaatata gtaaataata tctattcttt
     4081 ctataatggt tcattgtaca tggagttatt gtatgatttt acgcatgatg agttagtaca
     4141 taaccctata atcgaaagta cggttgatac aattcagtca gttgaatttg aaagagtttc
     4201 tttttcatat aatggtaagg ataatgtaat aaacgatata agtttttcaa ttaaagcagg
     4261 agagaggtta gcaattgttg gtgaaaatgg ttctggaaaa agtacgattt tcaaattagc
     4321 ctgcggtctt tacgataatt atgaaggcaa tatatatata aatggtatta atcttcgttc
     4381 aatacagaaa aaatcatact acaagagaat atccgcattg ttccaagatt ttttaaagta
     4441 tgaattgaca ctgcgtgaaa atgttggatt gggtgaatta tcaaagttat atagtgatga
     4501 ggatttaata agtgcactga agtatacagg agttgattcg atattttact ttaatcagga
     4561 aaaattaaat cagatagatc tggaacagca attagggaat tggtttgagg atgggagaca
     4621 gttatctggt ggacagtggc aaaaaattgc attatcaaga gtatatctca aagaagcgga
     4681 ttgctatttg ctagatgagc ctagttctgc gttagatcct gagtctgaaa caaaaatttt
     4741 taaaacattt tttgaattat cgcaaaataa aattggtatt tttattacgc ataagccaag
     4801 cattactcaa tctgtgaata aaattttgta tctagataat ggatgtatat ctgaaacagg
     4861 aagtttcgct gatttaaata ttaaaggaat gaagtttaaa aacttattag ataaagaaat
     4921 tggagccaca tatgacacct caaattaaac agatagtaga ttacattgca gatagtttta
     4981 taaattatga acagatagta gctgaaagtg agaataaagt actaattaat attgaatcta
     5041 aatatagaaa tacctttttt gataaatctc tgagtcatgg tgtgccatca ttaatcttaa
     5101 tgtatagttc actatataaa gtgacaaaag atgagagata tctaacattc tccaacatat
     5161 acgttgagaa attagtagac ataatctcta gagatggtat agaatctccg tctctctatg
     5221 ctggcactgc aggtattgca ttagctatta aagaagcgag tatatctgga aagtattatc
     5281 cgaaattatt atcatcctta aattgtcttc taaaagaaca aattaaagac aaattggtag
     5341 taagtttttc taatattgag aaaggaatta ttgagccata tgattatgat atggttaacg
     5401 gatttagtgg aataactaat tacttactcc ttgaacaaga atttttttct gtagagttaa
     5461 atcaaattgg aaattatctt ttgaaatata ttgagacaat tttaaataag gtaactaatt
     5521 gttctgaaga tagagaagct gaatttgatc taggaattgc acatggaatt actggtccta
     5581 tgctaatcct agcaaaatta aaatcagaaa aaattttgag tgtagaagta ggagatatac
     5641 tgaataaagc tataaattta atgtttttat ttagaaggga tgacaaacta tggcctggaa
     5701 aaatttatac tagtaatatt ttggatcttg atttaaatta tcttcctact agaatggcat
     5761 ggtgttacgg tacgcctgga actgcagttg ccctttttaa aatgtgtcaa ttgatagatt
     5821 taaattttat tgacgatatt aatgaaagtt taattgctca gatgagaagt tcagacaaaa
     5881 atgtctattc tcttacaatg tgccatggtt tggcaggagt aacttttata tatgacttga
     5941 ttggtaaaaa ttattcaagt gagaggctaa ctcgctttgc agaagagctt cgccaacaaa
     6001 taattacatc gttttcaact acaaaagtat ttgggtatac tgacagagaa aaatttggag
     6061 aaatagaagt tgaactggag tcaataggga tattgcaagg ggtatcaggg atagtgttat
     6121 tcttattaaa tccttacagt gacgaggagc tattgtggca aagaatattt atttaatttg
     6181 aaaaggagaa caaaatgtat aaggtatttc caactggtat gattcgtatt ccattgcaaa
     6241 aacaagaatt ttataataaa gactatgaag atttaataaa tgataaagtt tttatggaac
     6301 aacttttagt tgctagtccc agtctttatg atagtgtaat agattttgat aaatgtaatg
     6361 aaaagaaaaa gcaaaatgta aaagtctcat taaatagtta ttctaaaaga gcagcgttta
     6421 gaactactcc atttggtatc tttactgcta ttaatacagt tgatttgaca aagggaacca
     6481 cttctaatgt acaaaaggtt agttttatta aaaaagcagt cccagattat tcatggatat
     6541 attctctagt aaaatcgtat gaaataagta accttgaaaa actttcatta aaaataaata
     6601 cagcggcgtt tactcaaggt gacagatatg tactaccatt tacagtgaat gaatcagagg
     6661 aagatagaaa cataagtttt tcaaaaccaa ttaagttact tgtagaaaaa tgtaaaactc
     6721 gatatataaa atatgaagag ttaatagatg tatttaaaaa taattatcca gatatagtgt
     6781 cagacatgct agagtcatat atacacgact tagttacaaa tgatttttta atttcagatt
     6841 taagaccacc aatttgtaat atcaattcct tagattatct actatctaaa ttagaagaag
     6901 gaacactatg tactgactta actactttaa agaagatgat tgaagattac aacgatttaa
     6961 atattgggga aggtagagac ttacttttgc agatttttga taaaatggaa agtatttata
     7021 aatatgataa cggtggaaat tacttaaaag ttgattcatt agcagagtat gaaggcgatt
     7081 ttaatattct atctgaaacg gctcgggaaa gtatagaacg actcgcagag atgtttgtaa
     7141 aattaaatac aaatgtaaag agaagagggg ggaggaaaaa ttcacttgaa gagtatcagt
     7201 tgaaatttat tggcaaatat ggagaaaact gctcaattcc ctttgttgaa gtaataaata
     7261 aagatgcagg cataggtttt cctgaatatt acaaaggagg agaaggtgaa gcaatagagt
     7321 taagcgatcc aataatgcaa atgtttgaaa agaaatatga ggaagctcta ttaaatggag
     7381 ggcatatcga attttattca aaagatttag atgattttca aacagatcaa agcaatagtt
     7441 tagattcgtt tgaattaaat tttaatataa aaataataaa taatgatgtg aagttatatt
     7501 taggagctaa tatcggttct ggacaagcag gtagaagctt tggaagattc tatggattaa
     7561 gcgaaacagt aagagaaaca attaagaatt taaaccatca aaataatacg aatgtagagt
     7621 tgtcatttgt tcctaaacag ataagacttg cgaatgtaat tcaaaattat agtgatgagt
     7681 cttataatac ttcatttttt accacatcat gggattcaga gaacgaactg cgattggagg
     7741 atatttatat aagatatagt gatggtaagt ttcattttac aactatcgat gggaaaaatg
     7801 agttgaaatt taccatgaat aatatgctaa attccgatag ccaaagcagg gttttgagat
     7861 tgttggttga tttatctgag tttgagtacg gtttatcgca ttggtcatta tttccttggg
     7921 atattttggc tcaggaaaga gtatatattc ctgaaatttt atttgaagac attacgattg
     7981 ccactgctca gtggaattta tcagtaatta gagaagagat tatacatcaa aaaatattta
     8041 ctggtaaaaa agatatattc aataagttta gaaagactta taagatacct aaagatatta
     8101 ttttgaaata tgcagataat gaattgaggt tagatttatc tatagatagt gatttagaga
     8161 ttttatttaa aaacattaat aaatataggt ttgtttcttt gagaggaatt gagcagggag
     8221 agtctatttt taaggataat ttagggaact acaatacaga gatagtagtt ccaattctta
     8281 aggaatctaa tggaataaac aatacaaacg ataatgttca atttaaaaat gaagttaaga
     8341 aaatatattt accattttgt gactggctat ttttcaaatt ttatggtcca aaagagcgac
     8401 aagaagaatt actaaataac tggaaaaaat tttggctaaa cctgccatca gaactgttta
     8461 gccaaagtaa catgttttat atgagatata atgataccaa tgatcatata agaattcgga
     8521 taaattgtga ttcgattgaa aataactttt ctttgtattt gtcagtagtt caagatctat
     8581 tacctcaact gattgagagt tgtattatct ctgacataga agtgagtagc tataaaccag
     8641 aagtaaatag atatggagga cctcatttaa taagttatgc agaggaaatt ttttgtaaag
     8701 aatctatctt atttcttaat aatacaatag aattttcaga tgatgaaaga ttagtttgtg
     8761 ctacttactt ggttctatat tatctaaatc actttttcaa agataaagaa acaaggcgct
     8821 ctttcttatt tgagaattat accggaaagt ataagaaaga tttcaaaaat ttgccaattg
     8881 atttacctat tgagtatatg aagattttga atggagtcac ctcagctctt gatagatatg
     8941 attttttcca agaaatggat agatatctca tgtcttatat gaaagagtat aatcgatttg
     9001 gtggtacaaa tgatatctat aatactaaat tcaatttagt aggaagtttt ttacatctat
     9061 caatgaatcg attaaacggg attgatagag agtttgaaga gaaagtgtat tgttttgctt
     9121 actatacact aaacgctcag caatatatag aatgggagta actaatatgt tgaaaataca
     9181 aaatctaaaa aaatcttatg gtaagagaac catattgaat aatgtcaata tgaatattcc
     9241 taagggaaaa gtgtatgctt taatcggtcc aaatggtgct ggaaaatcaa ctattatgaa
     9301 aattttgaca ggcttagtta gtaagacaag tggttctatt atttttgaag gtagagaatg
     9361 gtcacgtcgg gatctgcgaa aaatcgggag tattattgaa gaaccaccac tttataaaaa
     9421 tttgagtgct tacgataata tgaaggtagt tacaacaatg cttggtgttt cagaaagcac
     9481 tatacttcca ttattaaata aagttggtct aggaaatatt gacaagagac cagtaaaaca
     9541 attttctctt ggaatgaagc aacggttagg tatagctatc tctttaataa attcacctaa
     9601 actacttata ttagacgaac ctactaatgg cttggaccca attggaattc aagaattaag
     9661 ggaaattata gagtcattta aatcagaagg aatgacaatt atgatttcaa gccatatact
     9721 gtcagaagtt gaacatctag ctgattttat tggatttatc tatgaaggaa agattattct
     9781 ggaaaaagaa tatgacggct ctgagaatct tgaagagtta ttcaataatc aaattttatt
     9841 tgaaaagagg aggtaggggc tatggtacaa atttttttat caaatttatt aaaaataaaa
     9901 aatacgagta ttagcaagtt gattcttatt ttccctatta tttgtgtgat gttatcttta
     9961 ttgttttcag cattaggtgg tgaaaatatc ctacgtttaa ctgcggaaac aacagtaaat
    10021 cagtggggag ttatttggat taatgttatt attgctgtaa attctggttt gttaaataaa
    10081 ttagaattag attctaataa gtattctgtt tttttatcaa gggatgttga tttaaaaaag
    10141 gtagaatatg ccagagttct attgatagct cttataaatc taataattag tatgatttta
    10201 agcttaatgc ttattgtaat tagttttgtc ctacctactc caagtcttat tagtataggt
    10261 aggatactat taactatctt gttaatttgg ttgactacac tatggcaaat cccgtttatt
    10321 ttatggctat caagaaaaat taatgtgtat tttgctatga ttattaatat tatatctcca
    10381 ctaattattg gtacaagttt ttctctctta aataaatggt atttgttccc ttatgattgg
    10441 tcgttgaagt tgcttgagcc aatgacaaga atgagaataa atagtatacc ttttggagcg
    10501 gagtttgttc cagactactc acagattttt atatcattgt tcctaggaat tgcttttttc
    10561 atcttactga ccaatctatt tgctatctct ttcaaaaaac aggtgaaata gtcatgaaat
    10621 catatttatg ttctaatttt ttaaaaataa aaaatacttc atatttacta atacatattt
    10681 tagctgcact attatttccc ttacttctat ttttctattg gagtcaacgt ggaggtttac
    10741 aaagcaggac tgtattattc tcctactttc aaatagtagg tgtacttctt ccttttgttg
    10801 caagtattgt gtgcattcaa ctaaaaaatt tagaagaatc atctggaaaa tataaatatt
    10861 tattgggtta ctcacagtcg aattataaac catttattgt agaattagta tttctatggt
    10921 tatgctattg tatagtatta attatttcaa ttactatatt tattctttta ttgaaaacta
    10981 ttggtataaa tgtatctctc agactactta ttttgaatag tttaatttat atcatttttg
    11041 cctatgtaac ctatctgatc aatcatatta ttagctatat atttagtaca ggtgtggcat
    11101 taggtatttc aatggtaggt gttattgctg cagcattttg tgaaacgagc cttggtgata
    11161 aggtatggtt tcttattcca tgggcatggc ttttaagaat atcagatact ctatataacc
    11221 aacagaaaat ggcaattgtt ccacttattg ttatattttt tgtttcatgc acagtagctt
    11281 tacttcatat acgaatattt aaaagttgga atcaagactg tttgacaagt agttaaatac
    11341 atctattttg tttacctagc aataaatatg attttttaag ttataaatac tatctaactt
    11401 gtttaactaa aaaatgacta taaaatttca atggattact agcttatatt taatagtctt
    11461 gtccaaatag gacaattaaa cattttttaa tcaaatctcc tttatactga tattaggaga
    11521 atgttagatg tatccgcgta ttaggaattt gagagaagat aacgatttca ctcaaaagtt
    11581 tgttgcaaat ttactttcct tctctcatgc aaattatgca aaaattgaga gaggagaggt
    11641 tgccttaatg gcagatgttc ttgtgcagtt ttctaaactc tataacgtca gtattgatta
    11701 tttattaggg caaacagatt atccgtatcg ttttcataat aaataaaagt ctcgccttga
    11761 tttgttcatt gacaactgaa taaaccgagg tgggactttc ttgtttgtcg agcaatttag
    11821 tgagatacgc cgtgatagga gcgataatta ttagctataa aatagagtgg ttctctatct
    11881 gccatgacca caaataggga caatgatact tctgaacgtt caggctgcca gcgtaaaagg
    11941 ccagtgggtg agattcccat gagtgattta accagtcata cccacggagg ttgcaatttt
    12001 tttgaaagga gtggaggtgt gaaaatagta acatataaaa
//
