LOCUS       BGC0000557             11650 bp    DNA     linear   BCT 24-JUL-2016
DEFINITION  Streptococcus pyogenes FF22 lantibiotic (scn) gene cluster region
            containing: scnK, scnR, streptococcin A-FF22 precursor (scnA),
            scnA1, scnM, scnT, scnF, scnE, scnG genes, complete cds, and tnpA
            gene, partial cds.
ACCESSION   BGC0000557
VERSION     BGC0000557
KEYWORDS    .
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
COMMENT     On Oct 9, 1997 this sequence version replaced gi:153556.
            ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-07 19:51:46
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..11650
                     /db_xref="taxon:1314"
                     /mol_type="genomic DNA"
                     /note="FF22 lantibiotic (scn) gene cluster region and tnpA
                     gene"
                     /organism="Streptococcus pyogenes"
                     /strain="FF22"
     protocluster    1..11650
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[3049:5833]"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      3050..5833
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
     cand_cluster    1..11650
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(LANC_like and DUF4135)"
                     /kind="single"
                     /product="lanthipeptide-class-ii"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..11650
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000557"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..11650
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lanthipeptide-class-ii"
                     /region_number="1"
                     /rules="cds(LANC_like and DUF4135)"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(244..1605)
                     /gene="scnK"
     CDS             complement(244..1605)
                     /codon_start=1
                     /gene="scnK"
                     /gene_functions="regulatory (smcogs) SMCOG1003:sensor
                     histidine kinase (Score: 115; E-value: 8.4e-35)"
                     /gene_kind="regulatory"
                     /product="ScnK"
                     /protein_id="AAB92598.1"
                     /transl_table=11
                     /translation="MNIFKKNILKFILSFFFIIIIDVILLITATNYIRSQQSASTIIET
                     VSSEITLSSNGKYNVSSKAKQLIDDKKLWIMIIDKDNGKEKFNINKPQIIENQFDFADA
                     IRFSRFYLKDYPVFTQIKDNDIYIIAFPKDSIIRYSSNYFELNRVQIFPILILSIIFAN
                     CLFCLFLYIYSVTFLNRNIKPIINAITKLPNGINTQVKSVRELDKLTLAINSANQQLRE
                     NEEFKENWISGIAHDIKTPLSVIVSNTSLAIEKTNDDEFLKHLKPTLVESHYIQNLLND
                     LNIFARLTNGNLKLNHEIVDIIPFFKEIIIQIINQEIWEDFNFEFIPDSKLLGKKMYIE
                     KALISRVVHNLIYNSVLHNHSGCDIKIILEYLPEDCFSITIRDNGIGTSPERLKNINKI
                     EEFNFDMSGVRRSGMGLKISKQIIDLHDGNMIITSTQGQYFQTVITLPIESLNT"
     gene            complement(1623..2321)
                     /gene="scnR"
     CDS             complement(1623..2321)
                     /codon_start=1
                     /gene="scnR"
                     /gene_functions="regulatory (smcogs) SMCOG1008:response
                     regulator (Score: 184.3; E-value: 4.1e-56)"
                     /gene_kind="regulatory"
                     /product="ScnR"
                     /protein_id="AAB92599.1"
                     /transl_table=11
                     /translation="MNFESQLLNKRILIVDDDVALSKSIKEVLISRDFKNISCAYSISE
                     GIDIFSVSKIDLIILDVMLPDGEGHLLAKYIRKYSDVPILFLTAKNNPDDEIRGLESGG
                     DDYVTKPFLPKSLTYRIIALLRRAYKDESELIELTNCTIDLNNASVEKNGQHLSLTPTE
                     IQILRKLFSNKNYIASTESICDTVWGLESLGYEKSLMVHIRNIREKIEITPSKPNHLIT
                     VKGLGYKLVI"
     regulatory      2426..2431
                     /gene="scnA"
                     /regulatory_class="minus_35_signal"
     gene            2426..2674
                     /gene="scnA"
     regulatory      2452..2457
                     /gene="scnA"
                     /regulatory_class="minus_10_signal"
     regulatory      2506..2510
                     /gene="scnA"
                     /regulatory_class="ribosome_binding_site"
     sig_peptide     2519..2593
                     /gene="scnA"
     CDS             2519..2674
                     /codon_start=1
                     /function="antibacterial"
                     /gene="scnA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) L_biotic_A"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) leader_d"
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /note="lantibiotic"
                     /product="streptococcin A-FF22 precursor"
                     /protein_id="AAB92600.1"
                     /sec_met_domain="L_biotic_A (E-value: 5.9e-30, bitscore:
                     92.5, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="leader_d (E-value: 3e-08, bitscore: 23.1,
                     seeds: 16, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEKNNEVINSIQEVSLEELDQIIGAGKNGVFKTISHECHLNTWAF
                     LATCCS"
     CDS_motif       2519..2593
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence: MEKNNEVINSIQEVSLEELDQIIGA"
                     /locus_tag="scnA_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       2594..2674
                     /prepeptide="core"
                     /locus_tag="scnA_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="5.20"
                     /molecular_weight="2778.2"
                     /monoisotopic_mass="2776.3"
                     /core_sequence="GKNGVFKTISHECHLNTWAFLATCCS"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MEKNNEVINSIQEVSLEELDQIIGA"
                     /leader_location="[2518:2593](+)"
                     /alternative_weights="2796.2"
                     /alternative_weights="2814.2"
     mat_peptide     2594..2671
                     /gene="scnA"
                     /product="streptococcin A-FF22"
     gene            2874..3029
                     /gene="scnA1"
     CDS             2874..3029
                     /codon_start=1
                     /gene="scnA1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) L_biotic_A"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) leader_d"
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /product="ScnA1"
                     /protein_id="AAB92601.1"
                     /sec_met_domain="L_biotic_A (E-value: 7.1e-27, bitscore:
                     82.6, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="leader_d (E-value: 1.6e-07, bitscore:
                     20.9, seeds: 16, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEEKMCLGALNALQEFQIEELDNLLGGRGHGVNTISAECRWNSLQ
                     AIFTCC"
     CDS_motif       2874..2954
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence: MEEKMCLGALNALQEFQIEELDNLLGG"
                     /locus_tag="scnA1_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       2955..3029
                     /prepeptide="core"
                     /locus_tag="scnA1_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="3.80"
                     /molecular_weight="2594.9"
                     /monoisotopic_mass="2593.2"
                     /core_sequence="RGHGVNTISAECRWNSLQAIFTCC"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MEEKMCLGALNALQEFQIEELDNLLGG"
                     /leader_location="[2873:2954](+)"
                     /alternative_weights="2613.0"
     gene            3050..5833
                     /gene="scnM"
     CDS             3050..5833
                     /codon_start=1
                     /gene="scnM"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: DUF4135"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: LANC_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1070:lanthionine synthetase C family protein (Score:
                     702.6; E-value: 1.5e-212)"
                     /gene_kind="biosynthetic"
                     /product="ScnM"
                     /protein_id="AAB92602.1"
                     /sec_met_domain="LANC_like (E-value: 3.1e-69, bitscore:
                     222.7, seeds: 47, tool: rule-based-clusters)"
                     /sec_met_domain="DUF4135 (E-value: 2.1e-81, bitscore:
                     263.3, seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNPKELLYSQFDRFPKVVIENNFPELLNESSELIKDVEDEISDYY
                     RSTLIYLINEKRIEKNLIGDSPESRYEYFNNVLCQNGLIFEEIDRRFPSINQRVMSTIK
                     KCLELINFVKERFTLDFKELRETGYIYSEAQTPKISEVKIKITGDIHNGCGVCILSYEE
                     QKVVFKKKSSNPNVLLHELNIEVGKFLQKDIDFIPDFLDKGEYFWEKFVSSSPLRTEED
                     AKEFYRRMGYLLSYSYILNISDLHFENLISTSFSPKLVDVETVFSVSPYQTVANNESTL
                     EIINNSRNSILSTGLLPVSEAGKVFGGDTSGVLGGTLIGEAKIVINHNRDDIHVEKQKF
                     KTENQDHLPYFIDSKGMKEFLNAEDYVEYIKEGFREVSYFFMNSQDFLKKLYIKHNDIK
                     TRILFRNTRDYSLVRQLLVSPVYCEQSEILFETMANKLSEQNSRSLCLSEKKQLLNMDI
                     PYFYSNIDSCDIKDENMIIWNLESSALSEAINKLEKLSEEIINEQIELIEFSIKTPKAL
                     YSTELQEAYQKFEKVSSSENIIKTGIDTLVDIILENESNSLKDDSTNWLTLKVTDYDAF
                     ELVPMDDSLYEGLSGIAISLSEAYDFLDSGRQRRVKECLKRIFSVLSNSYMKLPNHSFF
                     VGKLGIYSALKRISVVTGQEIQNSIMNYNNLKYTLDVDVLSADFLSSFPNEITALRNSD
                     IKIDNLTQALDKLKELAIVQKDFISWDKLESNNVSLAHGNLGVEIALLYLAGKLESPEA
                     LNLFHKAKMFDKHQKLENGWIDKRNSSTSANWCHGSTGVLVARLAQLKLDDEYSLLSYS
                     ERIELENDMKHAAKQILEIGFDMTNFSLCHGTSGNLLALTYYQSYLTGADSEKLKEILD
                     REYRKLHSFGLENGWMCSFNTKYNVYGLMTGVSGILFSTVKYMKGDDSLDVLIPNF"
     gene            5856..7925
                     /gene="scnT"
     CDS             5856..7925
                     /codon_start=1
                     /gene="scnT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288:ABC
                     transporter related protein (Score: 137.6; E-value:
                     9.2e-42)"
                     /gene_kind="transport"
                     /product="ScnT"
                     /protein_id="AAB92603.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 6.5e-41, bitscore:
                     128.7, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNIILQNNEEDCLLACYTMLLNDLGHKVPLYEIYDKDTLPADGLN
                     VSYLLSLSDRFGVKINAYHASFDELLKVYGEKKQRMILHWNNDHFVVLEKITSNKTVIV
                     DPAIGRIKYSRDEFLTHYSETMVSVNKRNNFHPQTYKKIFWKYFKQTLQLKPIALFLLS
                     LLFIQVSVLLFSVILRQMLAEDFKFGISLFLLGAVLIFQLLGYFIKNGALDRYNTDFDK
                     YYSKELFQRLLQKPLLYFRNHLSGGVSEKISFKSTLRDNVTLKIIPSCVSLISAIIIFA
                     YLMTISIKLSVILILMIASYSIISTLLYHRQNEYNQTYLQYLIDFNSELQTDLDDIDYI
                     KIMRREKTVFHSWMEQNNQVTKKYPQILKIENLSQLIGTIFNYISLSSIIVIAVYYKGY
                     INLSLPDLLVYQTSISLLISQIEQVKGAVFEEIRLGVYAEKQSDLLKETSPIGVPSSQS
                     DDYLIKTKNLNFSYGKNPIYSDVNLTISKGEKIAIVGKSGSGKSTLLLMLAGITYDGSL
                     EYGIEKFKDNLRVVLQNMTPRKGTVLENLEWTLDDVTPLYQVLNDTTADEVIARLPNKI
                     HSRLLKQGKNLSGGQIQKLLIAKSLLKENSIIFWDEAFSNLDEQSKNKIYDNVLQNNRY
                     SDRTMLIVSHHLDIVNYVDSIIFIDNETGKVIKDTHEHLMETNVNYTNFITSKN"
     gene            8037..8936
                     /gene="scnF"
     CDS             8037..8936
                     /codon_start=1
                     /gene="scnF"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 166.4; E-value:
                     1.4e-50)"
                     /gene_kind="transport"
                     /product="ScnF"
                     /protein_id="AAB92604.1"
                     /transl_table=11
                     /translation="MKNAIELRKLSKVYQDITTVDLEKITVREGEIYGFLGPNGAGKTT
                     TMKMILSLVSPTLGEILINGEDIKGNHAYLRVIGSMIEEPSYYPNLTGYENLLVFQKMV
                     GFDESNIWPTLALVGLAEENNRKKLVKAYSLGMKQRLALAFALVKKPKILLLDEPTNGL
                     DPAGIHEIRELIVKLAKEKGLTVFISSQILSEIEHIADRVGIINHGRLVYEGEIEAIKS
                     NTWIEIGGDFSQNNIVQSLINYGQVEVLGASASHVKFKNIDNDKLADVGTYLIENDYRI
                     FRVVRESENLEDIFPLFN"
     gene            8948..9712
                     /gene="scnE"
     CDS             8948..9712
                     /codon_start=1
                     /gene="scnE"
                     /product="ScnE"
                     /protein_id="AAB92605.1"
                     /transl_table=11
                     /translation="MIKAIQIELLKSKRTKSFMISFIMMLVAATWSLLAASRSIDIPKL
                     RTIGLFFNNLQANSIFLPIATCLFVSRIVGNEKEGSTFKLQEANNTNLIIIFTRKLIFT
                     NSVFLLLNIFQVLIVYLNVLRYDIQVPIWTLILQVVGLTMSSFTLITLFLYLSIILEKQ
                     GILLGIGFLSGFFGMITSQTLKWINLIFPFGGSSFLALYRIKILNSSDSLDYIFSWDKS
                     VPLNYTLYLIYCVMIYSVVKYLLKKKKGDNNE"
     gene            9728..10441
                     /gene="scnG"
     CDS             9728..10441
                     /codon_start=1
                     /gene="scnG"
                     /product="ScnG"
                     /protein_id="AAB92606.1"
                     /transl_table=11
                     /translation="MAKNSQRSYFSSGIIFLVLSSFIGLANFFLNYEVLIEEQMSRVLW
                     GQLTFYNSQLLFPAMLAIFSGMIILPEFERKTFEMLRANQVSVKKMLLSKFLLALLLIT
                     VIQVLLFVIYLVTLKLSQIPFVAKDLLLFLRATVLSVIGSSSVVMIHSYIMAKTKNFSK
                     SVGIAAIGSFCGFIFIMLGGVINQFFPYSQPMIGIRSRALVDMSISEFVIFIVVNALYS
                     FIFFKLTLRTLEKNG"
     misc_feature    10502
                     /note="the genome sequence of Streptococcus pyogenes SF370
                     differs from that of strain FF22 upstream from this base;
                     strain SF370 does not contain the scn lantibiotic cluster"
     misc_feature    10503..11650
                     /note="this region is present within the genome sequence of
                     Streptococcus pyogenes SF370; the open reading frame of
                     tnpA is completed within this sequence"
     CDS             complement(10555..>11650)
                     /codon_start=2
                     /gene="tnpA"
                     /gene_functions="other (smcogs) SMCOG1026:transposase
                     (Score: 275.3; E-value: 7.6e-84)"
                     /gene_kind="other"
                     /product="TnpA"
                     /protein_id="AAB92607.1"
                     /transl_table=11
                     /translation="KQSLVNLYQTGKTRSELCKDYGVSASALAKWIKQYSQVKLEDNSV
                     LTAKQIQGLQKRNAQLEEENLILKKSKCHIHAKLKVRLLAVYRLRFEHATTTLCRVLRV
                     NRSTYYKFLKHKPSKRELDNQIYKKQILEIYTKANKRLGVKSIKVILQRDYDTKISEGR
                     IYRLMKNMALPKMATVKPKTALKRTQKTYPQNLLNQKFNPDKPNQVWSTDFTYISIGYK
                     KYVYLCAILDLYSRKCIAWKLSHHMDAKLACDTLELALNKRKIEGTLLFHSDQGSQFKA
                     RELRKIIDDNTIMHSFSKPGYPYDNAVTEAFFKYLKHRQINRKKYQNIKQVQLDCFEYI
                     ENFYNNYNPHTANLGLNPNQKEEN"
     gene            complement(10555..>11650)
                     /gene="tnpA"
ORIGIN
        1 ctagatagga atagacttgc tttttaatag atcttagtga tttatttgca gtagtgtcta
       61 tcacaccaaa tactaaataa tgatttttaa aaattttttc atagttgata ttatctccga
      121 taataatatt tgcaatcgtg aagaaagagc ctaaaaaata aatattctga ataacagaaa
      181 gctaaagtca ttatttttta cttatgtttc ggattttaat ctctgcttta tcaaaaaatt
      241 ttgttaagtg tttagtgatt caatagggag ggtaattact gtttggaagt actgcccttg
      301 tgtacttgta attatcatgt ttccatcatg taaatcaata atttgtttag aaattttcaa
      361 tcccatacca ctccttcgaa cgcctgacat atcaaaatta aattcttcaa tcttattgat
      421 attttttagt ctttcaggag aagttcctat accattatca cgaatagtaa tcgaaaaaca
      481 atcttcaggt agatattcta aaatgatttt aatgtcgcaa ccagaatgat tatgaagtac
      541 tgaattataa attaggttat gaacaactct agaaatgaga gctttttcaa tatacatttt
      601 cttacctaat agtttagaat caggaatgaa ttcaaaattg aagtcttccc aaatttcttg
      661 attgataatc tgaatgatta tttctttgaa gaaagggatt atatcaacaa tctcatggtt
      721 tagttttaag tttccgtttg ttaatcgagc aaatatattt aaatcgttta ggagattttg
      781 aatgtagtga ctttcaacta aggtgggttt caaatgttta aggaattcgt catcatttgt
      841 tttttcaatg gctaaggaag tgttagatac aattactgaa agtggtgtct ttatatcatg
      901 tgctattcca gaaatccaat tctccttgaa ttcttcgttt tcacgaagtt gctgatttgc
      961 tgagttaata gcaagtgtta atttatctaa ttctcgtaca gatttgacct gagtattaat
     1021 tccatttggt aatttagtaa ttgcgttaat gataggttta atgttacgat ttaaaaaagt
     1081 aacagaataa atgtaaagaa ataggcagaa caaacagttt gcaaatatga tagacaaaat
     1141 aaggattgga aaaatttgaa ctctatttag ttcgaaataa ttactactat aacgtattat
     1201 actgtctttt ggaaaagcaa tgatgtaaat atcattatct tttatttgcg taaatacagg
     1261 atagtctttt aggtaaaatc tagaaaatcg tatagcatct gcaaaatcga attgattttc
     1321 tataatctga ggtttattaa tattaaattt ttcttttcca ttatctttgt caataatcat
     1381 tatccataac tttttatcat caatgagttg tttagcttta gaactaacat tatatttacc
     1441 attagaagat agagtgattt cagatgagac agtttcgatt atagtagatg cagactgctg
     1501 acttcggata taattggtag cagttattaa cagtatgacg tcaattatta taataaagaa
     1561 aaatgatagg ataaatttta gtatattttt cttgaaaata ttcatgctat attccttata
     1621 gtttatataa ctagtttata accaagccct tttacagtaa taagatgatt tggcttagat
     1681 ggagttatct cgattttttc tctgatattg cgaatatgta ccataagtga tttttcgtag
     1741 ccaaggctct caagtcccca aacggtatca caaatcgatt cagttgatgc aatataattt
     1801 ttatttgaaa ataattttcg taggatttgt atttctgttg gagtgagtga caaatgttga
     1861 ccattttttt caacacttgc attgtttaag tctatggtac aatttgttag ctcaattagt
     1921 tccgattcat ctttataagc ccttctcaac agtgcaataa tacgatatgt taaagatttg
     1981 ggaagaaatg gcttcgtaac ataatcatcc cctccggatt caagtcctcg aatttcatca
     2041 tcaggattgt ttttggctgt taaaaagaga attggcacat ctgaatattt cctaatgtat
     2101 tttgcaagta agtgcccttc tccatctggt agcataacat ctaaaataat gagatcaatt
     2161 ttagaaactg agaagatatc aattccttcg cttatactat aagcacagct gatattcttg
     2221 aaatcccgac tgataagtac ctctttaatt gatttgctaa gtgcgacatc atcgtcgaca
     2281 atcaatatgc gtttattcaa taattgagac tcaaaattca tacaatacct cctaaagtgt
     2341 aataaaatta taccataaag attatagata taagagaata agtaggactt gtgttgtata
     2401 atcttaaaat taaatttatg ttttctttat tctgtcttaa agttatcatc ttaaaataag
     2461 gttgtaacaa agaggaaggc acctatcctt ctgaagaaag ttaaatgagg tatagaaaat
     2521 ggaaaaaaat aatgaagtaa tcaactctat tcaagaagtt agtcttgaag aactcgatca
     2581 aattatcggt gctggaaaaa atggtgtgtt taaaacaatt tctcatgagt gtcatttgaa
     2641 tacatgggca ttccttgcta cttgttgttc ataaaactct actgttcagg tgtctttgat
     2701 gttacataaa aaaagagggg gacctctttt tttatttgca ttgacatagg tagaaaattt
     2761 aatttaactt tagttttcct taatattgtc tttatgtaac aaatttaaaa tcaatgtata
     2821 aagaaaacaa atttcgagtt ctactcgatg tcatataaat gaggtaaaga attatggaag
     2881 aaaaaatgtg cctaggtgct ttgaatgcgt tacaagaatt tcagattgag gaattggata
     2941 accttttggg aggaagaggg catggtgtca atacaatttc agctgaatgt cgttggaata
     3001 gtttacaggc aatttttact tgttgttaat atgtttagga gtaaagaata tgaatccaaa
     3061 agaactgtta tatagccaat ttgatagatt cccaaaagta gttattgaaa ataatttccc
     3121 agaattgcta aatgaaagta gtgagctgat taaggatgtt gaagatgaaa tttctgatta
     3181 ttatcgttcc actcttattt acctaataaa tgaaaaaaga attgagaaaa acctaattgg
     3241 agattctcca gagtcacgct atgaatactt taataatgta ctatgtcaga atggcttaat
     3301 ttttgaagaa attgatagac gtttcccttc tattaatcag cgtgtaatga gtactatcaa
     3361 gaaatgtttg gaattaatta attttgtaaa ggaaagattt actttagatt tcaaagagtt
     3421 gagagagaca ggctatatct atagtgaagc tcagactcca aaaattagtg aagtcaaaat
     3481 caaaataact ggagatattc acaatggttg tggagtctgc attttgagtt atgaagaaca
     3541 aaaagtagtt tttaagaaaa aatcatcaaa tcccaatgtt ctcttgcatg aactgaatat
     3601 agaagttggt aaattcttac agaaagatat agactttatc cctgattttt tagataaagg
     3661 agagtatttt tgggaaaaat ttgtatcaag ttctccgtta agaaccgagg aggatgcaaa
     3721 ggaattttat cgtaggatgg gttacttatt atcatattct tacatattga atatctctga
     3781 tttgcatttt gaaaatctga tttctacaag cttttctcct aagttagttg atgttgagac
     3841 agttttttca gttagcccct atcaaactgt tgcaaataac gagtcaacat tggagataat
     3901 taataatagt agaaattcga tattatcaac aggtttgctt cctgtttctg aagctggtaa
     3961 agtttttgga ggagatacta gtggtgttct aggaggcacc ttaattggag aggcaaaaat
     4021 cgtcatcaat cataaccgag atgatattca tgttgaaaaa caaaagttta aaacagaaaa
     4081 ccaggatcat ttgccttact ttattgattc gaagggaatg aaagaatttc ttaatgctga
     4141 agattatgtt gaatatatca aagaagggtt ccgtgaagtc agctactttt tcatgaattc
     4201 acaagatttt ttaaagaagc tttatattaa gcataatgat ataaagacta gaattttgtt
     4261 cagaaataca agagattata gcttagtgcg ccaattgcta gtttctcctg tttattgtga
     4321 gcaatccgag attttatttg aaacaatggc caataaattg tctgaacaaa atagtcgtag
     4381 tttatgtcta tcagaaaaga aacagctttt aaatatggat atcccttatt tttactcaaa
     4441 tattgatagt tgtgatataa aggatgaaaa catgattatt tggaaccttg aaagttctgc
     4501 tttaagcgaa gcaatcaata aattagaaaa attgagtgaa gaaatcataa atgagcaaat
     4561 tgaattaatt gagttttcta tcaaaactcc taaagcttta tatagtacgg aacttcaaga
     4621 ggcatatcaa aaatttgaga aggttagtag cagtgaaaat ataataaaaa ctggtattga
     4681 tactctagtt gatattattt tggaaaatga aagcaactct ctaaaagacg atagtacaaa
     4741 ctggctcacc ttgaaagtta cagattatga tgcttttgag ttagttccga tggatgactc
     4801 tctttatgaa ggtttgtctg gaatagcaat ttctttatca gaggcttatg attttctaga
     4861 ctcaggaaga caaagaaggg ttaaagaatg tctaaaacga attttttcgg tattatctaa
     4921 ttcctatatg aaattaccaa atcattcttt ttttgtgggg aagctgggaa tttactcagc
     4981 tttaaaacga attagtgttg ttacaggaca agagattcaa aattctatca tgaattataa
     5041 taatctcaaa tatacattgg atgtagatgt tttaagtgct gattttctat caagttttcc
     5101 taatgaaatc actgctttga gaaattcgga tattaagata gataatctta ctcaggcact
     5161 agacaaactt aaagagttgg caattgttca aaaggatttt attagttggg ataagttaga
     5221 gtcgaataat gttagtcttg cacatggtaa tttaggagtt gaaatagcgt tactttattt
     5281 agctggaaag ttagaaagtc ctgaggcact aaatttattt cataaagcaa aaatgtttga
     5341 taaacatcaa aaattggaaa acggctggat agacaaaaga aattcctcga ctagtgccaa
     5401 ctggtgtcat ggttcaacag gagtattagt tgcaaggtta gcacagttaa aactagatga
     5461 tgaatatagc cttctttctt attctgaacg gatagaatta gagaatgata tgaagcatgc
     5521 tgctaaacag attcttgaaa ttggttttga tatgacaaac ttttcacttt gccatggaac
     5581 cagtggaaat ttgctggcac ttacttacta ccaatcatat ttaacaggag ctgattcaga
     5641 gaagttaaaa gaaattttgg atagagagta tagaaaactg cattcttttg gcctggaaaa
     5701 cggctggatg tgtagtttta atacaaaata taatgtttat gggttaatga caggtgtgtc
     5761 aggaatatta ttttctacag taaagtacat gaagggtgat gattccctag atgtgttgat
     5821 tcccaatttc taagagatag acaggaatat gagatatgaa tattattttg caaaacaatg
     5881 aggaggattg tctcttagct tgttatacaa tgttacttaa tgatttagga cataaagttc
     5941 cattatatga aatttatgat aaagatacat tgcctgcaga tggtctaaat gtctcatatc
     6001 ttttgtcact aagtgacaga tttggggtga aaataaatgc ttatcatgcc tcttttgatg
     6061 aattactaaa agtatacgga gaaaagaagc aaagaatgat tcttcattgg aataatgatc
     6121 attttgtggt tcttgaaaaa attacgtcaa ataaaacagt tatagttgac ccagccattg
     6181 gtcgtattaa gtatagccgt gatgaatttt taactcacta ctcggaaact atggtgtcag
     6241 ttaacaaaag aaataatttt cacccccaaa catataagaa aatcttttgg aagtatttca
     6301 aacagacctt acaattaaaa ccgattgcat tattcttgtt gtcactcctg tttattcaag
     6361 ttagtgtttt actgttctca gtcattttaa gacaaatgtt agctgaagat ttcaagtttg
     6421 gtatcagcct attcctttta ggtgcagtat taatttttca attattaggt tattttataa
     6481 agaatggtgc attagataga tataatacag actttgataa atattacagt aaagagttgt
     6541 ttcaaagatt attacaaaaa cctttacttt attttagaaa tcatctaagt gggggagtat
     6601 ctgaaaaaat tagttttaaa tcaacactta gggacaatgt tactttaaaa attattccat
     6661 catgtgtaag tttgatttca gctattatta tctttgcata tctgatgact atatctatta
     6721 agttgtcggt gatattaatc ttgatgattg cttcatatag tataatctct actctattgt
     6781 atcataggca aaacgagtat aatcagacat atttacagta cttaattgat tttaactcag
     6841 agttacaaac agatcttgat gacattgact atataaaaat aatgagaaga gagaaaactg
     6901 ttttccattc ctggatggag caaaataatc aagtcactaa gaagtatccg cagattctta
     6961 aaattgaaaa tctttcacaa cttattggaa ctatttttaa ctacattagt ctatcctcaa
     7021 taattgttat cgcagtatat tataagggtt atattaattt atcccttcct gatctattag
     7081 tttatcaaac tagtatttct ttattgatat cgcaaattga acaagtaaaa ggggcagtat
     7141 ttgaagaaat tcgattgggg gtatatgcag aaaaacaaag tgatttatta aaagaaactt
     7201 cacctatcgg tgtacctagt tcacagtcag acgattattt aatcaaaact aaaaatttga
     7261 acttttctta tggtaaaaac ccaatctata gtgatgtgaa tttaacaatc agtaaaggag
     7321 aaaagattgc cattgtaggt aaatctggta gcgggaagtc aactttacta ttaatgttag
     7381 caggtatcac ctatgatgga tctcttgaat atggcatcga aaaattcaaa gacaatctaa
     7441 gagttgttct tcaaaatatg acacctagaa aggggactgt cttagaaaac ttagagtgga
     7501 ctttagatga tgtaactcca ctttaccaag tattgaatga tacaacagct gatgaagtta
     7561 tagcacgatt accaaataag attcactcta gactcttaaa acaaggaaaa aatttatcag
     7621 gtggacaaat ccagaagtta ctcatagcaa aatcactttt aaaagaaaat tcaattattt
     7681 tttgggacga ggctttcagt aatttagatg aacaaagtaa gaacaaaatt tacgataatg
     7741 ttttacaaaa caatagatat tcagacagaa ctatgttaat cgttagtcat catttagata
     7801 ttgtcaatta tgttgattct attattttta ttgataatga aactggtaag gttattaaag
     7861 atactcatga acatcttatg gaaacaaatg ttaactatac aaatttcatt acttccaaaa
     7921 attaagagaa tgtttctctt ttcttaatgt tatctttaag ttgcttagat acaataatgt
     7981 tgtaatagaa ggaaaggaag agactcattg aagggtctta ttatggggtt attaacatga
     8041 aaaatgctat tgaattaaga aaactttcta aggtctatca ggatattact acggtagatt
     8101 tagaaaagat tacggttcgt gaaggcgaga tttacggttt tcttggaccg aatggtgcag
     8161 gaaaaacaac aacaatgaaa atgattttgt cactagtttc tccaacacta ggagaaattc
     8221 ttataaatgg tgaagatata aaggggaacc acgcttattt gagggtaatc ggttcaatga
     8281 ttgaggaacc ctcctattat ccaaatttaa caggttacga gaatctacta gtctttcaaa
     8341 aaatggtagg ttttgatgaa agtaatatct ggccgacttt ggcattggtt ggtttagcag
     8401 aggaaaataa ccgaaaaaaa cttgtgaaag cctactcttt aggcatgaag caacggttag
     8461 ccttggcttt tgctctggtt aaaaaaccaa aaatcttgtt attagatgaa cctactaatg
     8521 gacttgatcc ggctggaatt catgaaattc gcgagttaat tgtaaaatta gctaaggaga
     8581 aagggctaac agtatttatc tcaagtcaaa ttttatccga aattgagcat atagctgacc
     8641 gagtaggcat tataaatcat gggagactgg tttatgaagg ggaaattgaa gcaataaaat
     8701 caaatacttg gattgagatt ggcggggatt tctcgcaaaa taatattgtt caaagtttaa
     8761 ttaattacgg acaggttgaa gtgctaggag cttcagcaag tcatgttaag tttaaaaata
     8821 ttgataatga caaattagca gatgtaggga cttatttaat tgaaaatgac tatcggattt
     8881 tccgagttgt tagggaaagc gagaatttag aagacatttt tcctctcttt aactaaggag
     8941 gtttgaaatg ataaaagcaa tacagattga attattaaaa agtaagcgta cgaaatcttt
     9001 tatgatttct ttcattatga tgttagtagc agcgacgtgg tcattgttag cagcctcaag
     9061 aagtattgat attcctaaat tacggactat cggattgttc tttaataatt tacaagctaa
     9121 ttctattttt ttacctattg caacttgcct atttgtttca cgtattgttg gtaatgaaaa
     9181 agaaggtagt acatttaaac ttcaagaagc aaataataca aatctaatta taatatttac
     9241 tcgcaaattg attttcacaa attctgtctt tctacttttg aatattttcc aagtcttgat
     9301 tgtttatcta aatgtactaa gatatgatat ccaagttcca atttggactc tgattttaca
     9361 agtagtaggt ttaacgatgt ccagttttac tttaatcact ctctttcttt atttatcaat
     9421 aattttagaa aaacaaggga ttcttttagg aattggattt ttatcaggtt ttttcggtat
     9481 gattacctcc cagactttaa agtggattaa ccttattttt ccgtttggag gatcgagttt
     9541 tttagcacta tatagaataa agattttaaa ttccagtgat agtttagatt atattttttc
     9601 ttgggacaaa agcgtgcctt taaattatac tttatacctt atttactgcg taatgatcta
     9661 tagcgtagtt aagtatttac tgaaaaagaa gaaaggggac aataatgaat gattatttca
     9721 aagcggagtg gctaaaaact cgcaaaggag ctattttagc agtgggatta tatttcttgt
     9781 tctatctagt ttcattggtt tggcaaattt ctttttgaat tatgaggtac tcattgagga
     9841 acaaatgagt agagtactct ggggacagtt aactttctat aatagtcagt tgcttttccc
     9901 agctatgtta gcaatattct caggcatgat tattctacct gaatttgagc gaaaaacatt
     9961 tgaaatgtta cgtgcaaatc aagtatctgt taaaaagatg cttcttagta agttcttgtt
    10021 agctttgcta cttataacag ttatacaagt attattattt gttatatatc ttgtaacact
    10081 gaaattgagc cagattccgt ttgtagcaaa agatttactt ttatttttaa gagcaactgt
    10141 gctatctgtt attggttcta gctctgttgt aatgattcat agttatatca tggctaaaac
    10201 aaaaaatttt tctaaatcag tcgggattgc agctataggt tcattttgtg gattcatatt
    10261 tattatgttg ggaggtgtca ttaatcaatt tttcccttat tcccagccaa tgataggtat
    10321 acgtagtaga gcgttagtag atatgagcat ttcggagttc gtaatattta tagtggttaa
    10381 tgctctatat agttttattt ttttcaaatt gacattacga actttagaga agaatggata
    10441 aagtggattt ttatacatta cagaagtaac taaatttata gaaggaaaag gtgagagcat
    10501 gtggactaat gtcaaataag tagacagaaa accgtgttat tttattacgt taaactaatt
    10561 ttcttctttc tgattagggt ttagtcctag attagccgta tgtgggttgt aattattata
    10621 aaaattctca atgtattcaa agcagtctaa ttgaacctgt ttgatatttt gatacttttt
    10681 tcggttgatt tgtctatgct ttaaatactt gaaaaatgct tccgttacgg cattatcata
    10741 aggatatcca ggtttagaaa aagaatgcat gatagtgttg tcatcaatta tttttctcaa
    10801 ttccctggcc ttaaattgtg acccttggtc ggaatgaaag agaagtgttc cttcaatctt
    10861 tcttttatta agagctaatt ctagagtgtc acatgctaac tttgcatcca tatgatgact
    10921 cagtttccaa gcaatacatt ttctagaata gagatcaagt attgcgcaga gatagacata
    10981 tttcttatat ccaatagaaa tataggtgaa gtcagtagac catacttgat ttggtttgtc
    11041 aggattaaat ttctggttga gtaagttttg aggatacgtt ttttgagtcc ttttaagagc
    11101 tgtttttggc ttaacggtag ccattttagg aagcgccata ttcttcatca gacggtaaat
    11161 tcttccttca gagatttttg tgtcgtagtc tctttgaaga atgactttga tagacttcac
    11221 accaagtctt ttgttcgctt tggtataaat ctcaagtatt tgttttttat aaatttgatt
    11281 atccaattct cttttggagg gcttatgttt tagaaattta tagtaagtgg agcgattgac
    11341 acgtaaaaca cgacacaagg ttgttgtggc gtgttcaaag cgtaaccgat agacagcgag
    11401 gagtcttact ttaagttttg catgaatatg gcacttgctt ttttttagga taaggttctc
    11461 ctcttcaagc tgcgcattac gtttttgtag cccttgtatt tgcttggcag taagtacaga
    11521 attatcttcg agtttgactt gggaatactg tttgatccat tttgcaagcg cagaagcgga
    11581 taccccatag tctttacaga gttcagaccg agtttttcca gtttgataaa ggttaacaag
    11641 agattgtttg
//
