LOCUS       BGC0000560             11100 bp    DNA     linear   BCT 16-JUN-2013
DEFINITION  Bacillus subtilis strain MMA7 subtilomycin biosynthetic gene
            cluster, complete sequence.
ACCESSION   BGC0000560
VERSION     BGC0000560
KEYWORDS    .
SOURCE      Bacillus subtilis
  ORGANISM  Bacillus subtilis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 13:50:42
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..11100
                     /country="Ireland: Gurraig Sound Kilkieran Bay, Galway"
                     /db_xref="taxon:1423"
                     /mol_type="genomic DNA"
                     /organism="Bacillus subtilis"
                     /strain="MMA7"
     misc_feature    1..11100
                     /note="subtilomycin biosynthetic gene cluster"
     protocluster    1..11100
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[2270:6728]"
                     /cutoff="20000"
                     /detection_rule="((LANC_like and (Lant_dehydr_N or
                     Lant_dehydr_C)) and not (YcaO or TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      2271..6728
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="((LANC_like and (Lant_dehydr_N or
                     Lant_dehydr_C)) and not (YcaO or TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
     cand_cluster    1..11100
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((LANC_like and (Lant_dehydr_N or
                     Lant_dehydr_C)) and not (YcaO or TIGR03604))"
                     /kind="single"
                     /product="lanthipeptide-class-i"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..11100
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000560"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..11100
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lanthipeptide-class-i"
                     /region_number="1"
                     /rules="((LANC_like and (Lant_dehydr_N or Lant_dehydr_C))
                     and not (YcaO or TIGR03604))"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            1038..1208
                     /gene="subA"
     CDS             1038..1208
                     /codon_start=1
                     /gene="subA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Antimicr18"
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /product="lantibiotic peptide"
                     /protein_id="AGL93171.1"
                     /sec_met_domain="Antimicr18 (E-value: 1.2e-08, bitscore:
                     23.8, seeds: 2, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEKNNIFDLDINKKMESTSEVSAQTWATIGKTIVQSVKKCRTFTC
                     GCSLGSCSNCN"
     CDS_motif       1038..1109
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence: MEKNNIFDLDINKKMESTSEVSAQ"
                     /locus_tag="subA_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       1110..1208
                     /prepeptide="core"
                     /locus_tag="subA_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class I"
                     /score="14.10"
                     /molecular_weight="3235.8"
                     /monoisotopic_mass="3233.6"
                     /core_sequence="TWATIGKTIVQSVKKCRTFTCGCSLGSCSNCN"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MEKNNIFDLDINKKMESTSEVSAQ"
                     /leader_location="[1037:1109](+)"
                     /alternative_weights="3253.8"
                     /alternative_weights="3271.8"
                     /alternative_weights="3289.8"
                     /alternative_weights="3307.8"
     gene            1296..2270
                     /gene="subP"
     CDS             1296..2270
                     /codon_start=1
                     /gene="subP"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075:alkaline serine protease, subtilase family
                     (Score: 154.5; E-value: 9.1e-47)"
                     /gene_kind="biosynthetic-additional"
                     /product="subtilisin-like protease"
                     /protein_id="AGL93172.1"
                     /sec_met_domain="Peptidase_S8 (E-value: 2.6e-28, bitscore:
                     87.8, seeds: 43, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNMTTNALPNQFSWNSYKVTNGYRLYDLQVGSPLIKIAVIDSGYD
                     PNHSEITGNIDKKKSCSFVDYENFNEDLYGHGTAVISVIVGKNQVKGVAPGITIISYKV
                     LDRNGFGKIEWVIHAILKAIEDCVHIINLSHVFTMFGLGNKGAFNKLQQAIDKARKKGI
                     IIVGAAGNQGVLLDMKSTSLYPTIKGINYVTSTNKNNYTSSYANIGSIIRFAAPSGELD
                     ETNNVTDLIIVAHPTNFPKTKFEKAIGLPDFYTVTCGTSISAAMFSSLIALVLTEYLIC
                     KQIHLSEKEILNIIQKNSSRVKDQNSIFEINAYSVIKFIKGLL"
     gene            2271..5381
                     /gene="subB"
     CDS             2271..5381
                     /codon_start=1
                     /gene="subB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_N"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lanthipeptide_LanB_RRE"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1155:Lantibiotic dehydratase domain protein (Score:
                     768.1; E-value: 2e-232)"
                     /gene_kind="biosynthetic"
                     /product="lanthionine synthetase B-like protein"
                     /protein_id="AGL93173.1"
                     /sec_met_domain="Lant_dehydr_N (E-value: 1.4e-134,
                     bitscore: 438.2, seeds: 73, tool: rule-based-clusters)"
                     /sec_met_domain="Lanthipeptide_LanB_RRE (E-value: 2.4e-10,
                     bitscore: 29.8, seeds: 20, tool: rule-based-clusters)"
                     /sec_met_domain="Lant_dehydr_C (E-value: 8.8e-34, bitscore:
                     106.0, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTKYLSSDLFMYRKPILDLSFYKECLAFDNEFNEEEYIAKISNL
                     LNTNPTIAEAIYFASEPLYNSFLNIEKLKKKKRKSVIDSMLKFLIRMSTRPTPFGLFSG
                     IGVETFSGQDEYLTKDSEVIKNTRLDMCWIYFLIRKLESNLSILEQLTVENNSLVYNHG
                     DRIKLSYQSLLHSDDNDQSLEMLTLRKTAVMDYVYSQAEFGINYSTLRDSINKTYSVTD
                     DISNDFLNSLLKNEVIITNLRTSLVNSDPFENILNIISKMHNVEEELNYLVRLKKSIEE
                     YKKSSGELGISILKKIKKEMRVIGYEGDDFLRTDLYYVRNKKRYINNNQKDSIEKLAEI
                     LSLLGSVNSFQPLEAYRDEFIDKYGIFQEVPLLELIDEELGIGLPPEYHENRHKPSQPE
                     PSNEFHRIKNIISSWQAEVSLSKNHEIKLDDEKIKHLFNSEELNNIAVSQELELYFNIL
                     LDEHKENKLYLNPNCGSNSLFQTYGRFKYFLGESDKEYIKQFSHIEKDVIFAEVTYYPT
                     HGKTANIINAETCFDYEIAFSCNSSKDNKHSIALSDLYVGSTYNFIYLKSKKLDKIIIP
                     KLSHMFNYSMGPKVYRFLVDIGVQYQKQWSNIDSFFYDAPIKPRVIYNNIVLSPKKWNL
                     NKSIIPFECDSGEKFHYYIKDWKKRYKVPDFIYLIEFDNKLLLNLENISHIKIIYNEFK
                     KLSNNQSLHFLEAEKELFNNHNNEIEEYVFTLKSKLHQLELDSYMTNIQSEMPFKYIGD
                     NERLKFLGTEWIYVKLYGLGTRENEFIADELGSFIKKHLDKGTIKSSFFIRYSDPKQHL
                     RVRFKVEKNNIFDTVLPIIIKWLEEMRTKGFVNYYCFDPYNPEIERYGGEQSYKIAEDL
                     FFCDSLIVSEWIQINRSGNVNIDEVTFGVFNILQLLKLFNLNIEEQMTWLEINTKPQKY
                     ANKFRGKKSTLIELADLIYTNKKLDKYNKISSIIEIREKTAITYSKSIENNPDFLIKNI
                     IMSVIHMHCNRLYGINRDKENLVIGLAYHTIKNYNNALKYNNVLIPN"
     gene            5406..6728
                     /gene="subC"
     CDS             5406..6728
                     /codon_start=1
                     /gene="subC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: LANC_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1140:Lanthionine synthetase C family protein (Score:
                     333.9; E-value: 2.2e-101)"
                     /gene_kind="biosynthetic"
                     /product="lanthionine synthetase C-like protein"
                     /protein_id="AGL93174.1"
                     /sec_met_domain="LANC_like (E-value: 3.7e-64, bitscore:
                     205.3, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQKCKNQDFLTDILLDIANSIKDKSETEKIILGDQNNPEDIIEVV
                     SLAAGYPGICVFLGYMDKVFPEDGWDVVAHDYLTDLISLIQTNKTYSLSLFNGLAGVLV
                     ALETCSRGGTRYTTIINQVLTLILENYKHYLSEDLLKLKKEGVSASEYDVISGWTGVGR
                     TLLHFDQISNKGDISLKKALHDIAEYLLLVSSIDTLSNMPNWLISNENLPLDIEKKTYK
                     KGYFNSGMAHGISGVLAFLSILKKENYKIKNLELGIKNIQNWIFEKVKTHKGIYYIPNV
                     IENEVVRNEAFFNHRDAWCYGTPGVSRAIFLSGIALNDQRVKMKALELFKTFFLRDEKD
                     RKIISPTICHGYAGILMLTLRMYKDTNDRFLADKMHDILNEMLTFYSQLNFSKFNNYEL
                     ISGKIVEEKKVGLLEGSTGIALVLISFIFGQDSWDKIFLIS"
     gene            6741..7331
                     /gene="subI"
     CDS             6741..7331
                     /codon_start=1
                     /gene="subI"
                     /product="hypothetical protein"
                     /protein_id="AGL93175.1"
                     /transl_table=11
                     /translation="MIQAVLEITIKFLTELISFELPVYMYIWFAVLFIFSMIVENVQLA
                     IAKDSILNSILKRLGIKILSLLVVLIIGVLGSNFFAQVVEDAVNKESNLAYYILSTTIA
                     TALTIIEPIIETMKKFIKEQSNLAFANFTVSVICLTVTFQAVSSIFSDYFILATPYKVV
                     LIISSAIGIVYLVKFDQNNLEVNSESVHSNKND"
     gene            7349..9106
                     /gene="subT"
     CDS             7349..9106
                     /codon_start=1
                     /gene="subT"
                     /gene_functions="resistance (resist) ABC_efflux (Score:
                     251.8; E-value: 8.4e-77)"
                     /gene_functions="transport (smcogs) SMCOG1288:ABC
                     transporter related protein (Score: 316.8; E-value:
                     4.7e-96)"
                     /gene_kind="transport"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="AGL93176.1"
                     /transl_table=11
                     /translation="MGNNHLLLLLKLVKWPKKILVYILFSLLFTTILSLVIPILTKNTV
                     DQFTDGTVNWALTIMLAGLFLINILLSGLSFYYLAFLGENATYYIRDKMFSHILDLPLT
                     FFDKNESGQIVSRVIDDVELMNTFISDKIPNFASQLIVVAGSLILLFVLDWKITLLMLI
                     SVPISLAVMIPLGNKMYKISLEKQNNMAIFTARISSAFSGIRLTKSYTAEKEEYGKGNV
                     ILKRIFKLNLKDTKIQAYMMPIMSTVILMIILLLVGYGGIRLSEGTISPGTFVAIIFYL
                     VQFVTPLSSLSNFFTEYKKTLGATKRLYEIYSLKPEAENKQISSNAFNLGNGDLKFENI
                     SFQYNDPSSEVFNKLNLRFPKNKITAVVGPSGAGKSTLFNLIERLYEAQNGEIYYESTP
                     IKDIDLTVWRKKIGYVMQDTLMITGTIKENLTYGLSGSVSQETIIKYSKLANAHDFIME
                     LPDNYETFIGERGIKLSGGQKQRIAIARALIKNPEILLLDEATSNLDSESEILVQQSIN
                     RLVRDRTTIIIAHRLSTIRDADNIVFLENGVVTGSGNHLDLINNHEKYREFVNGQLLNN
                     TSISASSLS"
ORIGIN
        1 tggtcgtttg ccgcatggtc caccggtccg acacagcaat ttcacctggc tacaatagaa
       61 ccggaaatgt caggtgtttt gcttagcatg aatcagtcaa tgatgcaatt cgccatggcg
      121 gtcggcgcag gtataggagg ggtttttgtg gaaaacgtat cattggcctc gattacctgg
      181 gttggtgcgt taggggttat gattgccatt attgcatcat tgctgatttt caattcgcaa
      241 ccgaaacaag cgctaaaaga tatcaatcaa taatttcgaa agttctaaca tccgctcgtt
      301 atacaagcgg gtgttttttt agcgttcaaa gtcagtccat gcatgagccc tccaagggcc
      361 gacatcgttc tgtatgtgtc gaaggcgcac tgaagctgaa tgagatttct taaggctttg
      421 ccgcggcgag ctgaagtaca gtacgattgc cttgatcgaa cactggaaac acaagagaac
      481 gtaaacctga acatccgcgt aaacgtcaag gaagttgcca cttggggagt aaacacttgc
      541 atcatctcgc tgaaaggcct agacaatgcg gatgacagat tcgtattgcc ggaagtaaac
      601 acagcgcttg ctctgtttcc gttgtcgatt gcagctgatt gcttactaat gctgccctgc
      661 atctcggttg tgatgtcgat aagcagcgta accttgtgaa gagtgttact gtggagtaag
      721 atttttaacc cctttggaat atagatcaaa aggtttttta atacttataa actgaaggag
      781 atcaatttta aatcgtttta aaactattaa aggaagttta gtaacgataa cttaaacatc
      841 tggctaataa gattactgag gatacatcaa aagtcgaata gtaatagtag agagtaaaag
      901 taaccgtaag aagaattata acaattagtt agcaaataat taaaaaatgt cccaaaaaaa
      961 gtattgacta actttttttg ggatagtact attgaattgc aaccggttga aagtatcaaa
     1021 aaaaggagga aatggaaatg gagaagaata atatttttga cctagacatt aataaaaaga
     1081 tggaatctac tagtgaggtt tctgctcaaa cttgggctac aatcggaaaa actattgtgc
     1141 agtctgtgaa aaaatgtaga acatttactt gtggatgttc gctaggctct tgcagtaact
     1201 gtaactaatt aggggatgag cagagggagt taaaatacat actatattaa aactccctct
     1261 tcttctaaag acaaggagta aacaattcca taaatatgaa tatgactaca aatgctttac
     1321 ctaaccaatt ttcttggaac tcttataaag taacaaatgg atataggcta tatgatcttc
     1381 aagttgggag ccctttaatt aagatagctg taattgatag tgggtatgat cctaatcatt
     1441 ccgagattac tggaaatatt gataaaaaaa aatcttgttc attcgttgat tatgagaatt
     1501 ttaatgaaga tctatatggg cacggtacag ctgttattag tgtgattgta gggaaaaatc
     1561 aagttaaagg agtagctccc ggaattacaa ttattagtta taaagttcta gatagaaatg
     1621 gttttgggaa aatagaatgg gtaatacatg ctattttaaa agccattgaa gattgtgtcc
     1681 acattataaa tttaagtcat gtttttacta tgttcggatt gggaaataaa ggagcattca
     1741 ataaacttca gcaggccatt gataaagcca gaaagaaagg cataataatt gtaggggcag
     1801 cagggaatca aggagtttta ttagatatga agagtacaag tttataccct acaattaagg
     1861 gaattaacta tgtcacctca acaaataaaa ataattacac ctcatcttac gcaaatattg
     1921 ggtcaattat tcgatttgct gcaccatcag gggaacttga tgaaacaaat aatgttacag
     1981 acttaataat agttgcacat cccacaaatt ttccaaaaac aaagtttgaa aaagcaattg
     2041 ggcttccgga cttttataca gttacatgtg gaactagtat atcagcagct atgttttcga
     2101 gtctaatagc attagtattg actgaatatt tgatatgtaa gcaaattcac ctatcagaaa
     2161 aagaaattct taatataatc caaaaaaact cctcacgagt aaaagatcaa aattctattt
     2221 tcgaaataaa tgcttatagt gtaataaaat ttatcaaagg attgttataa atgaatacaa
     2281 aatatttaag ctcagacttg tttatgtata gaaagccgat attggactta tcgttttata
     2341 aagagtgttt agcttttgat aatgaattta acgaagagga atacatagcg aagataagta
     2401 acttattaaa cactaatccc actatagcag aggccattta ttttgcaagt gaaccactat
     2461 ataattcttt tttaaatata gaaaaattaa aaaagaaaaa gcgcaagagt gttattgata
     2521 gtatgctaaa gtttttaata agaatgagta caagaccaac tccatttggc cttttttcag
     2581 gtattggggt tgaaacattt tcaggtcagg atgaatattt aactaaagat tctgaagtta
     2641 taaaaaatac tcgtttggat atgtgttgga tctatttctt aataagaaaa ttagagagta
     2701 accttagtat tctcgagcag ttaactgtgg aaaataactc actggtttat aatcacggtg
     2761 atagaatcaa attaagttat caaagtctat tacacagtga cgataatgat cagtcattag
     2821 aaatgttaac ccttagaaag acagcagtaa tggattatgt ctattcgcaa gcagaattcg
     2881 gtatcaatta ctctacgtta agagatagta taaataaaac atatagtgtt actgatgaca
     2941 tatcaaatga ttttttaaat agtctattaa aaaatgaagt tattataact aacttaagaa
     3001 catctcttgt taattctgat ccttttgaaa atatattaaa cataattagt aaaatgcata
     3061 atgtagagga agaattgaat tatttagttc gattaaaaaa atcgattgaa gaatacaaaa
     3121 aatcttctgg ggaattaggt ataagtattt taaagaaaat caaaaaggaa atgcgagtaa
     3181 taggatacga aggggatgat ttccttagaa cagatttgta ttatgtgaga aataaaaaac
     3241 gatatattaa taataaccaa aaagatagta tagagaagct agctgagatt ctttctctat
     3301 taggatcagt aaactctttc caacctttag aagcctatag agatgagttc attgataaat
     3361 atggtatttt tcaagaggta cctctcctcg aactcataga tgaagaatta ggtattggtt
     3421 taccacctga atatcatgaa aacaggcata aaccatcaca accagaaccg agtaatgaat
     3481 ttcataggat aaaaaatatt atatcttctt ggcaagcgga ggtgagttta tccaaaaatc
     3541 atgagattaa attagatgat gaaaagatta aacatttatt taactcggaa gaattaaata
     3601 acattgcggt atcacaagaa ttagaacttt attttaatat tttacttgat gaacacaaag
     3661 aaaataaact ctatttaaat cctaactgtg gttctaattc tctttttcaa acatatggaa
     3721 gattcaaata ttttttgggt gaaagtgata aagagtacat aaaacaattc tcacatatag
     3781 aaaaagatgt tatttttgca gaggttacgt attatcctac ccatggaaaa actgctaaca
     3841 ttataaatgc tgaaacatgc tttgattatg agatagcttt tagctgtaat tcgtccaaag
     3901 ataataagca ctcaattgcg ttgtctgact tatacgttgg gtcaacatat aactttattt
     3961 atcttaagtc aaaaaagttg gataaaatca taataccaaa attaagtcat atgtttaatt
     4021 attcaatggg ccctaaagtt tatagatttc ttgttgatat tggtgttcag tatcaaaaac
     4081 aatggtctaa tatagatagt ttcttttatg atgctccaat taaaccgagg gttatatata
     4141 ataatattgt attatcacct aaaaagtgga acttgaataa aagtattatt ccatttgaat
     4201 gtgactctgg agaaaaattc cattactata taaaagattg gaagaagaga tataaggtac
     4261 ctgattttat atatttaata gaatttgata ataaacttct gctgaactta gaaaatatct
     4321 cacacattaa aattatctat aatgaattta agaagctttc gaataatcaa tcactacatt
     4381 ttttagaagc agagaaagag ttgtttaata atcataacaa tgaaatagaa gaatacgttt
     4441 ttactttaaa gtcaaagtta catcaattag aacttgattc ttatatgaca aatattcaga
     4501 gtgaaatgcc ttttaaatat attggtgaca atgaaaggct taaattctta ggtacagaat
     4561 ggatttatgt gaaattatat ggattaggca ctagagaaaa tgaatttata gctgatgaac
     4621 ttggctcgtt tataaaaaaa catttagata aaggtactat taagtctagc tttttcatta
     4681 gatatagtga ccctaagcag catttacgtg ttagatttaa agttgaaaag aataacattt
     4741 tcgatactgt actcccgatt ataataaaat ggttagaaga aatgagaaca aagggttttg
     4801 ttaattatta ttgtttcgat ccatacaacc ctgaaataga aagatacgga ggggaacaat
     4861 catataagat tgcagaagat ttattttttt gtgactcatt gattgtttct gagtggatac
     4921 aaattaacag atctggaaat gttaatattg atgaagtgac ctttggagtt tttaatattc
     4981 ttcaactatt aaaattattt aatttaaata ttgaagagca aatgacatgg ttagaaatta
     5041 atacaaaacc gcagaaatac gctaataaat tcagagggaa aaaaagtact ttaattgagc
     5101 tggcagactt aatctatact aataagaaac ttgataaata taataaaatc tcaagtatta
     5161 tagagatacg agaaaaaact gcaatcacct attctaagag catagaaaat aatccagatt
     5221 tcttaataaa aaatattata atgagtgtaa tacacatgca ttgtaacaga ttatatggaa
     5281 taaacaggga caaagaaaac ttagttattg ggttagcata tcataccatt aaaaattata
     5341 ataatgctct aaagtataat aatgttttaa tccctaacta aattttaatt gaaaggaaag
     5401 aattaatgca gaaatgcaag aatcaagatt ttttaaccga tatcctttta gatatagcta
     5461 attcaattaa agataagtca gaaaccgaaa aaataatact tggtgatcaa aataatcctg
     5521 aagatataat tgaagtagta tcattggcag caggatatcc aggaatttgt gtgtttttag
     5581 gttatatgga taaagttttc cctgaagatg gatgggatgt tgtggcacac gactatttaa
     5641 cagatttaat ttccttaatt cagactaata aaacatactc actatcccta tttaatgggt
     5701 tggcaggtgt tttagtggct cttgaaactt gctctagagg cggaacaaga tatactacaa
     5761 taataaatca agtgctaacc ctaattttag aaaactataa acattatttg tctgaggact
     5821 tgttgaagct aaagaaagag ggagtgtccg cttctgaata tgatgttata agtggttgga
     5881 caggtgttgg aagaacccta ttgcattttg atcaaatatc aaataaagga gatatttcat
     5941 taaaaaaagc attgcatgat atagcagaat atttactgtt agttagcagt attgatactc
     6001 taagtaatat gccaaattgg cttatatcca atgaaaacct tcctttagat atcgagaaaa
     6061 aaacttataa aaaaggatat tttaattctg gcatggcgca tggaatttct ggagttttag
     6121 catttttatc gattctgaaa aaagaaaact ataaaataaa gaatttagaa ttaggtatta
     6181 aaaacattca aaattggata ttcgaaaagg taaaaacaca caaaggaatt tattatatac
     6241 ctaatgtaat tgaaaatgaa gttgttagaa atgaggcttt ttttaaccac agagatgcct
     6301 ggtgctatgg tacaccaggt gtgtcaagag caatcttttt atcaggtata gctttgaacg
     6361 atcaaagagt taaaatgaaa gccctagaac tgtttaagac atttttttta cgtgatgaga
     6421 aagatagaaa aattatatcc cccacaattt gtcatggata tgccggcata ttaatgttga
     6481 ctctaagaat gtataaagat actaatgacc gatttctagc tgataaaatg catgatatac
     6541 ttaatgaaat gttaactttt tactctcaat taaacttttc caaattcaat aattatgaat
     6601 taataagtgg gaaaattgta gaagagaaaa aagtgggttt gttagaagga agtacgggaa
     6661 ttgccttagt tttaatttct ttcatttttg gtcaagattc gtgggacaag atatttctga
     6721 taagttaaag ggggataaaa ttgatacaag cagtactaga aatcactatt aaatttctaa
     6781 cggaattaat ctcttttgaa ttgccagtat atatgtatat ttggttcgca gttcttttca
     6841 tattttcaat gatagtcgaa aatgtacaat tggcaatagc taaggatagt atattaaatt
     6901 ctattttaaa aagattagga ataaaaattt taagtctatt ggtggtctta attattgggg
     6961 ttttaggatc aaactttttt gctcaggttg ttgaagacgc agtcaataaa gaatctaact
     7021 tagcttatta tattctgtct actacaatag ctacagcatt gaccataatc gaaccaataa
     7081 ttgagacgat gaaaaagttt ataaaagaac aatcaaatct tgcttttgca aattttacag
     7141 tcagtgttat ttgtcttact gtaacgtttc aagcagtctc aagcatcttt agtgattatt
     7201 ttattttggc caccccttat aaagtggtgt taataatttc atcagcaatt ggtattgtat
     7261 atttggtgaa attcgatcaa aataatttag aagttaatag cgaaagtgtt cattcaaata
     7321 aaaatgatta atctaagagg ttatacatat gggcaataac catttactat tactgcttaa
     7381 acttgttaaa tggccaaaaa aaatattagt atatatttta ttttcattac tatttacaac
     7441 tatactatcc ttggtaatac caatattgac taaaaatact gttgatcaat tcactgatgg
     7501 aacagtaaat tgggccttaa caataatgtt agcaggttta tttctcataa atattttatt
     7561 aagtggatta tctttttatt atctagcatt tttaggtgag aatgcgactt actatatacg
     7621 agataaaatg ttttctcata ttttagattt acctctaacg ttttttgata aaaatgaatc
     7681 tggtcaaatt gtaagtagag ttatagatga tgttgagtta atgaatactt ttatttctga
     7741 caagatacca aactttgctt ctcaattgat agtagttgcc ggatcattaa ttcttctgtt
     7801 tgtgctggat tggaaaataa cattacttat gttgatttct gttcctattt cattagctgt
     7861 tatgattcca ttggggaata agatgtataa aatttcttta gaaaaacaaa ataatatggc
     7921 aatatttact gcaagaataa gtagtgcatt ttcaggaatt agacttacaa aaagttatac
     7981 tgcagaaaaa gaagagtatg ggaaaggtaa tgtcatttta aaaagaattt ttaagcttaa
     8041 tctaaaagat actaagatac aagcttatat gatgcctatt atgtctactg ttatactaat
     8101 gataatatta ttgttagtgg ggtatggggg gataaggtta tcggaaggaa ctatttcacc
     8161 aggtaccttt gttgcaataa ttttttactt agttcaattt gttacacctc tttcctctct
     8221 gtctaacttt tttactgaat ataaaaaaac actaggtgca acaaaaagat tatatgaaat
     8281 atattcacta aaacccgagg ccgaaaataa acaaattagc agtaatgcat ttaacttggg
     8341 aaatggggat ctgaaatttg agaacatttc gtttcagtat aacgatcctt cttctgaagt
     8401 atttaacaaa ctgaatttgc gttttccaaa aaataagata acagctgttg taggtccttc
     8461 tggagctgga aaatcaactt tatttaattt aatagaacgt ttgtatgaag ctcaaaatgg
     8521 tgaaatctat tatgaatcaa ctcctataaa agacattgat ttgactgttt ggagaaaaaa
     8581 aattggttat gtaatgcaag atactttgat gatcaccggc acgataaaag agaacctgac
     8641 gtacggttta tcaggctctg tatctcaaga aactataatt aaatattcta aattagccaa
     8701 tgcccatgat tttattatgg agcttccaga taattatgaa acctttattg gagaaagggg
     8761 tataaaatta tcaggtggtc aaaaacaaag aattgcaata gcaagagcat taattaaaaa
     8821 ccctgaaata cttttattag atgaggcaac ttctaattta gacagtgaaa gtgaaatatt
     8881 ggtacaacaa tctataaata gactagttcg agatagaacc actattatta tagctcatag
     8941 attgtcaaca attagagatg cagataacat tgtattttta gaaaatggag tggttacagg
     9001 aagcggtaat catttggacc taataaataa ccatgaaaaa taccgagagt ttgttaacgg
     9061 tcaattgcta aataacactt caataagcgc gagttctcta agttaattgt gcataaataa
     9121 tgagaccact tgggtacact ttttatcggt tctataacca taaaaagcag ctgaactttt
     9181 agttcagctg ctttttgatt gaatcttgtg actagaaagt caaatgtgaa aataaaactg
     9241 ttccaaagtt aatcagaaat ttattttcat attgtatcct ttcagtatca cggtgaaagg
     9301 agcatcatat gaaaacatta tggaaagtcc taaaaattgt ttttgtcagc ttggctgctt
     9361 tggttttgct tgtatgcgtc tcggtattta tttatcacca tttccagcta aataaggagg
     9421 cggcactcct taaagaaaaa ggcacagtag tcgatgttga cggtaaaaag atgaatgtgt
     9481 atcaagaggg aagcgggaag gatacgtttg tgtttatgtc tggttcgggt attgctgcgc
     9541 ctgcttatga aatgaagggg ctgtacagca agttttcaaa agaaaataag attgctgttg
     9601 tagatcgggc aggttatgga tacagtgaag tgtctcacga tgacagagat attgatacgg
     9661 tattggaaca gacgaggaaa gcgcttatga aaagcggaaa taagcctcct tatattttaa
     9721 tgcctcattc gatatccggg attgaagcga tgtattgggc acagaaatat cctaaggaaa
     9781 tcaaggccat tattgcgatg gatattggat tgccccagca gtacgtcacg tataaattga
     9841 gcggagttga caggttgaaa gtgagagggt tccacctgtt aacctcgatt ggttttcatc
     9901 ggtttatgcc ttccgctgta tataatcctg aggtgatacg gcagtcgttt ttaactgatg
     9961 aagaaaaaga aatctataaa gccattaact ttaagcaatt ttttaatgca gatatggagc
    10021 atgagctttt acagtcttac caaaacggca gcaaatctgt gaatctgccg gcgccaaagg
    10081 aaactcccgt cttgatttta gatgcggtct ctgaccaaaa tagacattca aagtatgcta
    10141 tacaaaaccg aaaagattat gaagtgtttg cggctcaatt caatactgcc gatataaagg
    10201 aactgagggg aacacacagt atttatttat atcagcctga tcaaatatat aaactgtcca
    10261 tggagtttat gagaaaggtt cgctaggatg aagggttatc atattttaat cgttgaagac
    10321 gatgtgatga ttggtgattt gctgcaaaag attttgcagc gtgagggata tcgtgtgata
    10381 tggaaaacag atggagcgga tgtgctttcg gtgattcaga aggtggattt ggtcattatg
    10441 gatgtgatgc tgccgggtga agacgggtat caaatgtctg caaaaatcaa agagctgggg
    10501 ctgggcattc cggtaatttt tctttcggcc cgtaatgata tggacagcaa gcttcaaggt
    10561 ttacagatcg gcgaggatta tatggtaaag ccctttgatc cgagagagct gctattaaga
    10621 atgcggaata tgcttgagca tcattatggc acctttacgc aaatcaaaca tttgtatatt
    10681 gatgcggtaa cgaaaaaagt gttcaatgaa agcctgcatg atgaggtatt gtttactgcg
    10741 attgagcgga aaattttctt ttatttatat gaaaatagag acagtatcct gacaaaggaa
    10801 catttctttg aatatctatg gcagctcgaa gatagaaacc cgaatattgt caatgtgcat
    10861 attaaaaaaa tcagagctaa aatcaatgat caagcgggtg agatgattga aaatatatat
    10921 ggagaagggt atcggctgaa taccgttgtg aagaaatgaa gctcaagaca aaatatcagt
    10981 tgttattgtt tacggctgtc attagtgttc cgatgctgtt gctggcggtc agtgttttga
    11041 tgtctgttat ttatgacagc atgtttaaac cgatgaatca tggtatgccc tttcacaggt
//
