LOCUS       BGC0000564              8634 bp    DNA     linear   BCT 06-FEB-2015
DEFINITION  Clostridium botulinum A str. ATCC 3502 complete genome.
ACCESSION   BGC0000564
VERSION     BGC0000564
KEYWORDS    .
SOURCE      Clostridium botulinum A str. ATCC 3502
  ORGANISM  Clostridium botulinum A str. ATCC 3502
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-07 19:52:56
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 8634
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..183
                     /codon_start=1
                     /db_xref="InterPro:IPR019891"
                     /db_xref="UniProtKB/TrEMBL:A5HZ31"
                     /gene="SagA"
                     /gene_functions="biosynthetic (rule-based-clusters) LAP:
                     TIGR03602"
                     /gene_kind="biosynthetic"
                     /locus_tag="CBO0486"
                     /note="Similar to Streptococcus iniae bacteriocin-like
                     prepropeptide SagA SWALL:Q8GP18 (EMBL:AF465842) (54 aa)
                     fasta scores: E(): 6.2e-06, 50 38d in 52 aa, and to
                     Streptococcus pyogenes, Streptococcus pyogenes streptolysin
                     S associated protein SagA or spy0738 or spym18_0799 or
                     spym3_0480 or sps1374 SWALL:O69194 (EMBL:AF067723) (53 aa)
                     fasta scores: E(): 0.00037, 42 38d in 50 aa"
                     /product="putative cytolysin"
                     /protein_id="CAL82040.1"
                     /sec_met_domain="TIGR03602 (E-value: 6.4e-36, bitscore:
                     111.7, seeds: 2, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKDMLKFNEHVLTTTNNSNNKVTVAPGSCCCCSCCCCVSVSVGGG
                     SASTGGGAAAGQGGN"
     protocluster    1..8634
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[0:3764]"
                     /cutoff="20000"
                     /detection_rule="((((goadsporin_like or PF00881 or
                     TIGR03605) and (YcaO or TIGR03604)) or TIGR03882 or
                     TIGR03603 or TIGR03602) and not cyanobactin_synth)"
                     /neighbourhood="10000"
                     /product="LAP"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1..3764
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="((((goadsporin_like or PF00881 or
                     TIGR03605) and (YcaO or TIGR03604)) or TIGR03882 or
                     TIGR03603 or TIGR03602) and not cyanobactin_synth)"
                     /neighbourhood="10000"
                     /product="LAP"
                     /protocluster_number="1"
     cand_cluster    1..8634
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((((goadsporin_like or PF00881 or
                     TIGR03605) and (YcaO or TIGR03604)) or TIGR03882 or
                     TIGR03603 or TIGR03602) and not cyanobactin_synth)"
                     /kind="single"
                     /product="LAP"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..8634
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000564"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..8634
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="LAP"
                     /region_number="1"
                     /rules="((((goadsporin_like or PF00881 or TIGR03605) and
                     (YcaO or TIGR03604)) or TIGR03882 or TIGR03603 or
                     TIGR03602) and not cyanobactin_synth)"
                     /subregion_numbers="1"
                     /tool="antismash"
     sig_peptide     1..139
                     /gene="SagA"
                     /inference="protein motif:SignalP:2.0"
                     /locus_tag="CBO0486"
                     /note="probabilty 0.999, with cleavage site probability
                     0.381 between residues 47 and 48"
     gene            1..183
                     /gene="SagA"
                     /locus_tag="CBO0486"
     gene            348..1304
                     /gene="SagB"
                     /locus_tag="CBO0487"
     CDS             348..1304
                     /codon_start=1
                     /db_xref="GOA:A5HZ32"
                     /db_xref="InterPro:IPR000415"
                     /db_xref="InterPro:IPR020051"
                     /db_xref="InterPro:IPR029479"
                     /db_xref="UniProtKB/TrEMBL:A5HZ32"
                     /gene="SagB"
                     /gene_functions="biosynthetic (rule-based-clusters) LAP:
                     PF00881"
                     /gene_kind="biosynthetic"
                     /locus_tag="CBO0487"
                     /note="Similar to Streptococcus equisimilis SagB
                     SWALL:Q93SL4 (EMBL:AY033399) (316 aa) fasta scores: E():
                     5.8e-45, 44.17 38d in 292 aa"
                     /product="putative cytolysin-associated protein"
                     /protein_id="CAL82041.1"
                     /sec_met_domain="PF00881 (E-value: 9e-29, bitscore: 90.0,
                     seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLLKNLKKQKVKKQRNTDIGNKMLVHFNTQYSSISPYVDSTVIKT
                     PDTIIKGSLYSKDNRFLSEEYLLNYRTNNDNLGFRMGVSSFFQKDAALTFVNLDMEEDG
                     QNVIELPPSKNIRVGLSTAIKSRRSVRGYSSKSMSLNDVANIFYYTQGICDEVEPYNLE
                     GNKKKIKLRANPSAGGLYPIQLYVYMKNIKDLKDGIYTYYPYSHGLKPIEVNKELLKVD
                     NFAEFGVLNAENANLIVFYVYNFLKNSRKYGDAGFSYALIETGEMAQNLQLVSTALGYG
                     ACDIGGYEKQYIEEVLDIDGVLKHVVHMTVVGWEQGD"
     misc_feature    717..1286
                     /gene="SagB"
                     /inference="protein motif:Pfam:PF00881"
                     /locus_tag="CBO0487"
                     /note="Pfam match to entry PF00881
                     Nitroreductase,Nitroreductase family, score 34.0, E-value
                     3.7e-07"
     gene            1308..2390
                     /gene="sagC"
                     /locus_tag="CBO0488"
     CDS             1308..2390
                     /codon_start=1
                     /db_xref="InterPro:IPR009036"
                     /db_xref="InterPro:IPR019892"
                     /db_xref="UniProtKB/TrEMBL:A5HZ33"
                     /gene="sagC"
                     /gene_functions="biosynthetic (rule-based-clusters) LAP:
                     TIGR03603"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Cytolysin_C_RRE"
                     /gene_kind="biosynthetic"
                     /locus_tag="CBO0488"
                     /note="Similar to Streptococcus iniae SagC SWALL:Q8GP16
                     (EMBL:AF465842) (354 aa) fasta scores: E(): 2.6e-38,36.33
                     38d in 355 aa"
                     /product="putative cytolysin-associated protein"
                     /protein_id="CAL82042.1"
                     /sec_met_domain="TIGR03603 (E-value: 2.2e-140, bitscore:
                     457.0, seeds: 5, tool: rule-based-clusters)"
                     /sec_met_domain="Cytolysin_C_RRE (E-value: 4.3e-37,
                     bitscore: 115.7, seeds: 8, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNNTIYRLSNNLRIYDNGENEIRFRSGLWNYNEAVLDLSAETEN
                     FTKSFRKVIDNLKNNKGISVKDLDGYELNNEEKNNLISIIDSLNEAGMLCSEDEKDNDF
                     AMSKVLLGDFSYNLNKKEENNQKLLFISDNDYAKQTAKNLSDGMNTNLDLYSKENIDNI
                     ISKDLTSNLDALEKKSNIEDIKNQLKDYSAILICMSHINMNLFRNINRVSIELKKPIIM
                     SFIDGPFITILSTLPPKTGCLECFEQRILSRLEDHVLYHKFIESDFKPSDKSHKSIIPL
                     LNIMTNMVISEGFLINNFNTCKIEGRVLSIFVPTLEIQSQDLLRVPFCPACGNISKAKL
                     KEINISTRNIVDGILEDINS"
     gene            2406..3764
                     /gene="sagD"
                     /locus_tag="CBO0489"
     CDS             2406..3764
                     /codon_start=1
                     /db_xref="InterPro:IPR003776"
                     /db_xref="InterPro:IPR027624"
                     /db_xref="UniProtKB/TrEMBL:A5HZ34"
                     /gene="sagD"
                     /gene_functions="biosynthetic (rule-based-clusters) LAP:
                     TIGR03604"
                     /gene_functions="biosynthetic (rule-based-clusters) LAP:
                     YcaO"
                     /gene_kind="biosynthetic"
                     /locus_tag="CBO0489"
                     /note="Similar to Streptococcus iniae SagD sagD
                     SWALL:Q8GP15 (EMBL:AF465842) (453 aa) fasta scores: E():
                     9.8e-91, 51.54 38d in 454 aa"
                     /product="putative cytolysin-associated protein"
                     /protein_id="CAL82043.1"
                     /sec_met_domain="TIGR03604 (E-value: 7.2e-114, bitscore:
                     369.7, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="YcaO (E-value: 1.4e-60, bitscore: 194.9,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIKFSPSFNNILENFKSLAGNQSGILEAACMPVVNFNSDVSLKSI
                     TGNMPDYHKQVFNKDVSMQYHIMGYGSHYEEALIKYTGESIERYSSIMGPTLLKDKIVY
                     ESYKELSKNNKVMPLKYIDVFTENQIKKAKELNVSICDKKVEEDDIIGWIKCPSLLKKG
                     KEIYVPAKMMFVGYEEDKNKGEMTFIPSFSTGTAAHKSLKKALLNALVEYIQIDSFMIN
                     WHTKGKCSLVKIDDENVLKVLEDVNLGEKSPYEIIPLYMSLEECPIHNFGVFLRRKDNK
                     LPYLLFGIQGGLDASHALTRGIMEAASISYGAYFNAIYNKDMLNNVIKDDHKFLDLDSN
                     VLYYSLPNKIEEKNKLIEDFIGDEITLSSLNKLNIKGVDEQIKTLLNYLKDISEYAVYL
                     DLTPPETRDKGWYVMRAMIPELIEMCIPEFPFENHPRMKKYGGVINEYPHPMP"
     gene            3739..4377
                     /gene="sagE"
                     /locus_tag="CBO0490"
     CDS             3739..4377
                     /codon_start=1
                     /db_xref="GOA:A5HZ35"
                     /db_xref="InterPro:IPR003675"
                     /db_xref="UniProtKB/TrEMBL:A5HZ35"
                     /gene="sagE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="CBO0490"
                     /note="Similar to Streptococcus iniae SagE SWALL:Q8GP14
                     (EMBL:AF465842) (220 aa) fasta scores: E(): 1.9e-18,32.05
                     38d in 209 aa"
                     /product="putative cytolysin-associated protein"
                     /protein_id="CAL82044.1"
                     /sec_met_domain="Abi (E-value: 6.5e-14, bitscore: 41.5,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNIRTQCLNLLILLVTFNPIVLAQLFLNNEKSYNESLKRSFKALA
                     IIYTIILTSIFIISKERFYINIDNNYYGYIIAVMFVPITIISEIAVGYIMLGISNKTIP
                     KFKISLVFTKTNINVIILSVFIGICEEFAYRQLWFNILFKDFKMNIAIVLIITSFVYAL
                     NHIFLGKQVFSQKMVAGLIYGALFYFSGFNIIVPIITHSLENIAILLKG"
     misc_feature    order(3757..3816,3859..3912,3949..4017,4060..4125,
                     4186..4239,4267..4335)
                     /gene="sagE"
                     /inference="protein motif:TMHMM:2.0"
                     /locus_tag="CBO0490"
                     /note="6 probable transmembrane helices predicted for
                     CBO0490 by TMHMM2.0 at aa 7-26, 41-58, 71-93,
                     108-129,150-167 and 177-199"
     misc_feature    4087..4374
                     /gene="sagE"
                     /inference="protein motif:Pfam:PF02517"
                     /locus_tag="CBO0490"
                     /note="Pfam match to entry PF02517 Abi, CAAX amino terminal
                     protease family, score 33.6, E-value 4.6e-07"
     gene            4385..5113
                     /gene="sagF"
                     /locus_tag="CBO0491"
     CDS             4385..5113
                     /codon_start=1
                     /db_xref="UniProtKB/TrEMBL:A5HZ36"
                     /gene="sagF"
                     /locus_tag="CBO0491"
                     /note="Similar to Streptococcus iniae SagF SWALL:Q8GP13
                     (EMBL:AF465842) (229 aa) fasta scores: E(): 0.15, 26 38d in
                     150 aa"
                     /product="putative cytolysin-associated protein"
                     /protein_id="CAL82045.1"
                     /transl_table=11
                     /translation="MNLYLKILVFLLMTIYIVILYLPMNINYKKFKFTKKLCLNLSGAI
                     GTSPTNCWCLMKYVHLLIIGVIPIACFFYRGIVKNYSLYTETNIIKSFILIILSFIGIL
                     EISFFMIIVVVSYLMKKDSRKEMSNVSWVKFNKKYPFYTGILKPIMYTVFEILLYYYVM
                     FYVINDYLKVSVMYSILGIAILYSVSKLAFTKNKEQALIYGIWAFALNIIGSCIMIYSN
                     SIILTFILYLLYSFVIAFKE"
     misc_feature    order(4394..4462,4559..4618,4661..4729,4805..4873,
                     4901..4963,4982..5041,5051..5104)
                     /gene="sagF"
                     /inference="protein motif:TMHMM:2.0"
                     /locus_tag="CBO0491"
                     /note="7 probable transmembrane helices predicted for
                     CBO0491 by TMHMM2.0 at aa 4-26, 59-78, 93-115,
                     141-163,173-193, 200-219 and 223-240"
     gene            5422..6354
                     /gene="sagG"
                     /locus_tag="CBO0492"
     CDS             5422..6354
                     /codon_start=1
                     /db_xref="GOA:A5HZ37"
                     /db_xref="InterPro:IPR003439"
                     /db_xref="InterPro:IPR003593"
                     /db_xref="InterPro:IPR017871"
                     /db_xref="InterPro:IPR027417"
                     /db_xref="UniProtKB/TrEMBL:A5HZ37"
                     /gene="sagG"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 218.2; E-value:
                     2e-66)"
                     /gene_kind="transport"
                     /locus_tag="CBO0492"
                     /note="Similar to Streptococcus iniae SagG SWALL:Q8GP12
                     (EMBL:AF465842) (307 aa) fasta scores: E(): 2.7e-49,50.47
                     38d in 313 aa"
                     /product="putative cytolysin ABC transporter, ATP-binding
                     protein"
                     /protein_id="CAL82046.1"
                     /transl_table=11
                     /translation="MNIVEIKNLEKKFKNNTAVNNINLTVKEGQVFGFLGPNGAGKSTT
                     INMICGLLTPTKGEIFILGKKMDNKAKDLKNNIGIVPQDIAIYEDLTAYENVAFFGGLY
                     GVRGQELKERAKETLEFVGLLDRAKDFPYKFSGGMKRRLNIACALVHQPKLIIMDEPTV
                     GIDPQSRNHILESIKKLNERGCTIIYTSHYIEEVEQLCTDIAIIDKGTIIAKGKKDELV
                     EKYSDLNMVVINIKDSTKVNIKALKSIEGVVEVNIKENTIEITNKSSDNLDDIIMYFLN
                     NKIKIKNIENKHSDLETIFLKLTGKKLRD"
     misc_feature    5506..6051
                     /gene="sagG"
                     /inference="protein motif:Pfam:PF00005"
                     /locus_tag="CBO0492"
                     /note="Pfam match to entry PF00005 ABC_tran, ABC
                     transporter, score 231.0, E-value 1.8e-66"
     misc_feature    5527..5550
                     /gene="sagG"
                     /inference="protein motif:ProSite:PS00017"
                     /locus_tag="CBO0492"
                     /note="PS00017 ATP/GTP-binding site motif A (P-loop)."
     misc_feature    5692..5739
                     /gene="sagG"
                     /inference="protein motif:ProSite:PS00225"
                     /locus_tag="CBO0492"
                     /note="PS00225 Crystallins beta and gamma 'Greek key' motif
                     signature."
     misc_feature    5824..5868
                     /gene="sagG"
                     /inference="protein motif:ProSite:PS00211"
                     /locus_tag="CBO0492"
                     /note="PS00211 ABC transporters family signature."
     sig_peptide     6357..6495
                     /gene="sagH"
                     /inference="protein motif:SignalP:2.0"
                     /locus_tag="CBO0493"
                     /note="probabilty 0.995, with cleavage site probability
                     0.352 between residues 47 and 48"
     gene            6357..7490
                     /gene="sagH"
                     /locus_tag="CBO0493"
     CDS             6357..7490
                     /codon_start=1
                     /db_xref="UniProtKB/TrEMBL:A5HZ38"
                     /gene="sagH"
                     /locus_tag="CBO0493"
                     /note="Similar to Streptococcus iniae SagH sagH
                     SWALL:Q8GP11 (EMBL:AF465842) (375 aa) fasta scores: E():
                     1e-37, 30.58 38d in 376 aa"
                     /product="putative cytolysin ABC transporter"
                     /protein_id="CAL82047.1"
                     /transl_table=11
                     /translation="MNSMKILNILKKEILENFRDKKAMFLMTLFPMLLITILGTVFSGN
                     FASTINIPEINVMYSINKDVKIDKNFKDFTKEIQKNMKVNFKETKEEKDALEKISNGKA
                     DVYIKIPNDKKIYIYENNVRAFNSQLVSTLLDTFVDKVNMTKEVSSKNPMALSKIKVDN
                     SPNFVNTKTIDGKNSPRGIDYYAVTMLTMTILYGTAVGAMSIASEIKRRTYKRLICSNT
                     SPLKILFSKILASFLVIAFQSFLIYLFSKYILGTNWGNNKLALISVVASLIFMAVSAGV
                     CIANTIKNEGVMSIIIYMFVPVLTFLGGGYIPLDTIGSKMLNSASNISPLKWSNDAIFK
                     IIFGNDLSIFGTTMLINLGAGVLFLLIAILFPRKDVV"
     misc_feature    order(6429..6497,6906..6974,7035..7103,7146..7214,
                     7227..7295,7401..7469)
                     /gene="sagH"
                     /inference="protein motif:TMHMM:2.0"
                     /locus_tag="CBO0493"
                     /note="6 probable transmembrane helices predicted for
                     CBO0493 by TMHMM2.0 at aa 25-47, 184-206, 227-249,264-286,
                     291-313 and 349-371"
     sig_peptide     7492..7612
                     /gene="sagI"
                     /inference="protein motif:SignalP:2.0"
                     /locus_tag="CBO0494"
                     /note="probabilty 1.000, with cleavage site probability
                     0.305 between residues 45 and 46"
     gene            7492..8634
                     /gene="sagI"
                     /locus_tag="CBO0494"
     CDS             7492..8634
                     /codon_start=1
                     /db_xref="GOA:A5HZ39"
                     /db_xref="InterPro:IPR000412"
                     /db_xref="UniProtKB/TrEMBL:A5HZ39"
                     /gene="sagI"
                     /locus_tag="CBO0494"
                     /note="Similar to Streptococcus iniae SagI sagI
                     SWALL:Q8GP10 (EMBL:AF465842) (372 aa) fasta scores: E():
                     2.3e-37, 32.04 38d in 362 aa"
                     /product="putative cytolysin ABC transporter"
                     /protein_id="CAL82048.1"
                     /transl_table=11
                     /translation="MKQIFLLTKSILKRTFRKKSNIITFILLPLLAVFIALFLNASGNS
                     YSKIGVVDLNESYLSKDMIKSIDAKKNFVVVKISKNNMNKLLLDKRVDCVVVIPKDFNK
                     DIIDGNFKKLDIISVRGEDITSWIKNYLNYYIDTLNNISVASNKNEKDFKAIYDGYKKQ
                     DLKLNYKAIGDKTKTKSVTKQSIGLLLVFMLIASSTASSFILKDKYNKTYYRIFCSNTS
                     KSKYIIANVIANFVIFLIQIFVILFMSIYVLKLNFYIDTSIMFIILCSFGFVSIGFGII
                     IAAFSKSLNQSSYLSNILITPTCMLSGCFWPLDMMPSFMQKLANIFPQTWVLKTIDSLQ
                     QGKSFNEVISYLGIVILFALAFFVVGILKLTKDENLKSIF"
     misc_feature    order(7552..7620,8047..8106,8167..8235,8278..8346,
                     8365..8433,8533..8601)
                     /gene="sagI"
                     /inference="protein motif:TMHMM:2.0"
                     /locus_tag="CBO0494"
                     /note="6 probable transmembrane helices predicted for
                     CBO0494 by TMHMM2.0 at aa 25-47, 190-209, 230-252,267-289,
                     296-318 and 352-374"
ORIGIN
        1 gtgaaagata tgttaaaatt taatgaacac gtactgacaa ccactaataa cagtaataat
       61 aaggttactg tggctccagg aagttgctgc tgctgctctt gttgttgttg cgttagtgtt
      121 agtgtaggtg gcggttcagc ttcaacaggt ggcggagcag ctgctggaca aggtggaaat
      181 taataatttt atcttgtaca agcatctttc acagttcaat tatctaataa taatttaaac
      241 ccaggtagcc aggtgattat aatttaatat tattttatca cctggctaat atcatttaaa
      301 tatttaattt ctatttaaca tttaaaaata taaacaagga gctaaaaatg ttattaaaaa
      361 acttaaaaaa acaaaaagta aaaaagcaaa gaaatactga tataggaaat aagatgcttg
      421 ttcattttaa tactcagtat tctagtatat caccctatgt cgatagtact gtgattaaaa
      481 ctccagatac aataataaag gggagcttgt actctaaaga taacagattt ttaagtgaag
      541 aatatctttt aaattataga actaataatg ataatttagg ttttagaatg ggagtttcaa
      601 gtttttttca gaaggatgct gctctcacct ttgtaaattt ggatatggaa gaagatggac
      661 aaaacgttat agaacttcca ccttctaaaa atataagagt aggtttaagt acggctataa
      721 aatctagacg tagtgtcaga ggatattcta gtaaatctat gtctttaaat gatgtggcaa
      781 atatatttta ttatacccaa ggtatatgtg atgaagtaga accatataat cttgaaggta
      841 ataaaaagaa aattaaacta cgagctaatc catctgcagg agggttatat cctatacaat
      901 tatatgtata tatgaagaac ataaaagact taaaggatgg aatatataca tactatcctt
      961 attctcatgg tctaaaacct attgaagtaa ataaagaact tttaaaggta gacaattttg
     1021 cagaatttgg agttctaaat gctgaaaatg cgaatctaat tgttttttat gtatataatt
     1081 tcttaaaaaa ctcaagaaaa tatggtgatg ctggattttc atatgcctta attgagacag
     1141 gagagatggc tcaaaatctg caacttgtgt ctacagcttt aggttatggg gcttgtgaca
     1201 tagggggata tgaaaaacaa tacatagaag aggttttaga tatagatgga gtattaaaac
     1261 acgtagtaca tatgactgtt gtaggctggg aacaggggga ttaatttatg aaaaataaca
     1321 ctatatatag attaagtaat aatttaagaa tatacgataa tggtgaaaat gaaataagat
     1381 ttagaagtgg actttggaat tacaatgaag cggttttaga cttgagcgct gaaacagaaa
     1441 attttacaaa gtcttttaga aaagttattg ataatctaaa aaacaataag ggcatatcag
     1501 taaaggattt agatggatat gaattaaaca atgaagaaaa aaataattta attagtatta
     1561 tagattcctt aaatgaagct ggtatgttat gtagtgaaga tgaaaaagat aatgactttg
     1621 caatgtctaa agtgttatta ggagatttta gctataatct taataaaaag gaagaaaata
     1681 atcaaaagct attatttata tcagataatg attatgcaaa acaaactgct aaaaatttat
     1741 ccgatgggat gaatacaaat ttagatttat attcaaaaga aaatatagat aatataattt
     1801 ctaaagattt gacatcaaat ttagatgccc tagaaaaaaa atctaatata gaggatataa
     1861 aaaatcaatt aaaagattat tcggctatac taatatgcat gagtcatatt aacatgaatt
     1921 tatttagaaa tataaataga gtatctatag aactaaaaaa acctattatt atgtctttta
     1981 ttgatggacc ttttataact attttaagta cattaccacc taaaactggc tgtttagagt
     2041 gctttgaaca aagaatctta tcaagacttg aagatcatgt gttatatcac aaatttatag
     2101 aaagtgattt taaacctagt gataaatctc acaaatccat aattccctta ttaaatatta
     2161 tgactaacat ggtcatatct gaaggatttt taataaacaa ttttaatacc tgcaaaattg
     2221 aaggtagagt acttagtata tttgttccaa cattagaaat acaatctcaa gacctattga
     2281 gagttccatt ttgcccagct tgcggaaata tatcaaaggc taaattgaaa gaaataaaca
     2341 tatctactag gaatattgtg gatggaattt tagaagatat taattcttaa ctaggaggct
     2401 ttaacatgat aaaattttcc ccaagtttta ataatatatt agagaatttt aaatcattag
     2461 caggaaatca aagtggtata ttagaggctg catgtatgcc tgtagttaac tttaatagtg
     2521 atgtgtcttt aaaaagcata acaggaaata tgccagatta tcataaacaa gtatttaata
     2581 aagatgtgtc catgcaatat cacataatgg gttatggatc tcattacgag gaagctttaa
     2641 ttaaatatac cggtgaaagt atagaaagat attctagtat tatgggacca acactattaa
     2701 aagataaaat tgtatatgaa agttataaag aattaagtaa aaacaataaa gtcatgccct
     2761 taaaatatat agacgtattc acagaaaatc aaataaagaa agctaaggaa ttaaatgtgt
     2821 ctatttgtga taaaaaagta gaggaagatg acataatagg atggattaaa tgtccttcac
     2881 tattaaaaaa aggtaaagaa atatatgtac cagcaaagat gatgtttgta ggctatgaag
     2941 aggataaaaa taaaggtgag atgactttta taccatcctt tagtacagga acagcagcac
     3001 ataaaagttt aaagaaagcc cttttaaatg ctttagttga gtatattcaa attgattctt
     3061 ttatgattaa ttggcataca aaaggaaaat gttctttagt gaaaatagat gatgaaaatg
     3121 tgcttaaagt tttagaagat gtaaatttag gagaaaagtc tccctatgaa ataatacctt
     3181 tatatatgtc tttggaggaa tgtcctatac ataattttgg ggttttctta agaagaaagg
     3241 ataataaact tccttattta ttatttggta tacaaggggg actggatgct tctcatgctt
     3301 taactagagg gataatggaa gcagcttcaa tatcctatgg tgcatatttt aatgcaattt
     3361 acaacaaaga tatgttaaat aatgtaataa aagatgatca taaattttta gatttagatt
     3421 caaatgtgct atattattct cttccaaata aaattgaaga aaaaaataaa ttaatagaag
     3481 attttatagg agatgaaatt actctaagtt ctttaaataa attaaatata aaaggtgtag
     3541 atgagcaaat aaaaacttta ttaaattatt taaaggatat aagtgaatat gcagtatatt
     3601 tagatttaac tccaccagaa actagggaca agggatggta tgttatgaga gctatgattc
     3661 cggaattaat tgaaatgtgt ataccggagt ttccttttga aaaccatcca agaatgaaaa
     3721 aatatggagg ggtgattaat gaatatccgc acccaatgcc ttaatttatt aatattatta
     3781 gttacattta atccaatagt attagctcag ttatttttaa ataatgaaaa atcctataat
     3841 gaatctttaa aaagaagttt taaagcatta gctataattt atactataat acttacaagt
     3901 atatttatta tttccaagga aagattttat ataaatatag ataataatta ctatgggtat
     3961 attattgccg ttatgtttgt acctataact ataatttcgg aaatagctgt agggtatatt
     4021 atgttaggta tttctaataa aactattcca aaatttaaaa tatcattagt atttacaaaa
     4081 actaatatta atgtgattat attatctgtt tttataggaa tttgtgaaga atttgcatat
     4141 aggcaattat ggtttaatat tttatttaaa gattttaaga tgaatatagc tatagtttta
     4201 attattacat cttttgtata tgccttaaac catatatttt taggtaaaca ggtattttcc
     4261 caaaaaatgg tagcaggtct gatttatgga gctttatttt attttagtgg atttaatata
     4321 atagttccaa taataactca ctctctagaa aatatagcta ttctcttgaa aggttgataa
     4381 acttatgaat ttatatctta aaattttagt atttttattg atgactatat acatagtaat
     4441 actttattta cctatgaata ttaactataa aaaatttaaa ttcacaaaaa aattatgttt
     4501 aaatttaagt ggagctatag ggacatcacc aactaattgt tggtgtttaa tgaaatatgt
     4561 acatttatta attattggtg taatacctat agcatgcttt ttttataggg ggattgttaa
     4621 aaactatagt ttatatacag aaacaaatat aattaagtct tttatattaa taatattatc
     4681 ttttatagga attttagaaa taagcttttt tatgattata gttgttgttt catatttaat
     4741 gaaaaaagat agtagaaaag aaatgagcaa tgtgtcatgg gtaaagttta ataaaaaata
     4801 tcctttttat acaggaatac ttaaaccaat aatgtatact gtttttgaaa tattacttta
     4861 ttactatgta atgttctatg taattaatga ttatttaaaa gtatcagtta tgtattctat
     4921 tttgggtatt gcaatattat actcagtaag taaactagct tttacgaaaa ataaagagca
     4981 ggctttaata tatggaattt gggcctttgc tttaaatatt attggatctt gtataatgat
     5041 ttacagtaat tctataattc ttacatttat tctatattta ttgtattcat ttgtaatagc
     5101 ttttaaagaa taaatagagt tatttgtttt aaagggagat tttacgccat ctaaagctta
     5161 agaaatggtt agccaagtat gcgatcactt tttacgccat ctaaagcttt ggaaatgatt
     5221 ggctaagtac gtagctgatt tttatatttc cacttttaat aaaatggaag tattagagca
     5281 gtagtctata tataaaatac tataaatgct tattttatat ggttaatata ggataagttc
     5341 ataaagtatg gtatatgctg ctataacata aatgcttact ttgcatggtt actacagaag
     5401 atatataaag gagatgactt tatgaatatt gtagaaataa aaaatcttga aaagaaattt
     5461 aagaataata ctgctgttaa taatataaat ttaactgtta aagaaggaca agtctttgga
     5521 ttcttagggc caaatggggc aggaaaaagt actactataa atatgatttg tggactttta
     5581 actcctacta agggtgagat atttatttta ggtaagaaaa tggacaataa agctaaggat
     5641 ttaaaaaata atattggtat tgttccgcaa gacatagcta tatatgagga tctaactgcc
     5701 tatgaaaatg tagctttctt tggaggactt tatggtgtga gaggacaaga actaaaagaa
     5761 agagcaaagg aaactttaga atttgttggc ttattagata gagcaaagga ttttccttat
     5821 aaattttccg gcggtatgaa acgtagatta aatattgcct gtgctctagt acatcaacct
     5881 aaacttataa taatggacga gcctaccgtg ggaattgatc ctcaatcaag aaatcatatc
     5941 ttagaatcta taaaaaaact aaatgaaaga ggctgtacaa taatatacac aagtcattat
     6001 atagaagaag tagagcagtt atgtacagat atagccatta ttgataaagg aactattata
     6061 gctaagggca aaaaagatga gttagtagaa aaatactctg atttaaatat ggttgtaatt
     6121 aatattaaag attcaactaa agtaaatata aaagcattaa aatctataga aggtgtagtg
     6181 gaggttaata ttaaagaaaa tactattgaa attacaaata agtcctctga taatttggat
     6241 gatataataa tgtacttttt aaacaataaa ataaaaataa aaaatataga gaataaacat
     6301 agtgatttag aaactatatt ccttaaatta actggcaaaa aattaagaga ttagaggtga
     6361 actctatgaa gatattaaat atattaaaaa aagaaatact tgaaaatttc agagataaaa
     6421 aagctatgtt tttaatgacg ttgtttccta tgcttttaat taccatatta ggaacggttt
     6481 tttcaggaaa ctttgcatct acaattaata ttccagagat taatgttatg tattctataa
     6541 ataaggatgt aaaaatagat aaaaacttta aagattttac taaagagatt caaaaaaaca
     6601 tgaaggttaa ttttaaagaa actaaagaag aaaaagatgc tttagaaaaa ataagtaatg
     6661 gaaaagctga tgtgtatata aaaattccaa atgataaaaa aatatatatt tatgaaaata
     6721 atgtgagagc atttaattct caattagtta gtacgttgtt agatactttt gtggataaag
     6781 taaatatgac aaaggaagta tcctcaaaaa atccaatggc attgtctaaa ataaaagtag
     6841 ataattcacc taattttgta aatacaaaaa caatagatgg caaaaattcg ccaagaggaa
     6901 tagattatta tgctgttact atgcttacta tgactatttt atatggaact gcagttgggg
     6961 ctatgagtat tgccagtgaa ataaaaagaa ggacttacaa aagattaatt tgtagtaata
     7021 catcaccttt aaaaatatta ttttcaaaaa tattagcttc ttttttagta atagcctttc
     7081 aaagctttct aatatattta tttagtaaat atattttagg aacaaattgg gggaataata
     7141 aattagcttt gatttctgtg gtagcatctt taatatttat ggcagtatct gcaggagtat
     7201 gtatagctaa tactataaaa aatgaagggg ttatgtctat cattatatat atgtttgtac
     7261 ctgttttaac ttttcttggt ggaggttata tacctttaga tacaataggc agtaaaatgt
     7321 taaattctgc atcaaatata tcaccactta aatggagcaa cgatgccata tttaagatta
     7381 tttttggtaa cgatctgagt atatttggaa caactatgtt aatcaaccta ggggcaggag
     7441 ttttattctt attaattgct atattattcc caagaaagga tgtggtataa aatgaaacaa
     7501 atatttttat taaccaaaag tattctaaaa agaactttta gaaaaaaatc taatataata
     7561 acttttatat tacttccttt attagctgta tttatagctt tatttttaaa tgcttcagga
     7621 aattcttata gcaaaatagg agtagtagat ttaaatgaat cctatttatc taaagacatg
     7681 ataaagtcaa tagatgccaa gaaaaatttt gttgtagtta aaatttcaaa gaataatatg
     7741 aataaattat tactggataa aagagtagat tgtgtagtag ttattcctaa ggattttaat
     7801 aaagatataa tagatggaaa ttttaaaaaa ttggatataa tatctgttag aggagaagat
     7861 attacttctt ggattaaaaa ttatttaaat tattatatag atactcttaa taatataagt
     7921 gtggcatcta ataaaaatga aaaagatttt aaagctatat atgatggata taaaaagcaa
     7981 gatttgaagc ttaattataa agctatagga gacaaaacta aaactaaatc tgtaacaaag
     8041 caaagcatag gattattact tgtatttatg ctaatagctt ctagtacagc ctcatctttt
     8101 atattaaagg ataaatataa taaaacatac tatagaatat tttgttctaa tactagtaaa
     8161 agcaaatata taatagctaa tgtaatagct aactttgtga tttttttaat acagatattt
     8221 gtgattctat ttatgtccat atatgttctg aaacttaatt tttatataga tacaagtata
     8281 atgtttataa tattgtgttc ttttggattt gtatccattg gctttggaat aatcattgca
     8341 gccttttcca aaagtcttaa tcaatcatcc tatttatcta atatactaat aacacctact
     8401 tgtatgctat caggatgttt ttggccttta gacatgatgc caagtttcat gcaaaaatta
     8461 gctaatatat ttcctcaaac atgggtgctt aaaactatag attctcttca gcaaggaaaa
     8521 agttttaatg aggttatttc atatcttggt atagtaattt tatttgcatt agctttcttt
     8581 gtagtaggaa tactaaaatt aactaaggat gaaaacttaa aaagtatttt ttaa
//
