LOCUS       BGC0000567              7142 bp    DNA     linear   BCT 04-AUG-1993
DEFINITION  Rhizobium leguminosarum trifolii trifolitoxin (tfxA) gene and tfxB,
            tfxC,tfxD, tfxE, tfxF, tfxG genes, complete cds.
ACCESSION   BGC0000567
VERSION     BGC0000567
KEYWORDS    .
SOURCE      Rhizobium leguminosarum
  ORGANISM  Rhizobium leguminosarum
            Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
            Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium.
COMMENT     ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2022-11-07 19:53:22
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7142
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..7142
                     /db_xref="taxon:384"
                     /mol_type="genomic DNA"
                     /organism="Rhizobium leguminosarum"
                     /strain="T24"
     subregion       1..7142
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="RiPP"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..7142
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            597..725
                     /gene="tfxA"
     CDS             597..725
                     /codon_start=1
                     /gene="tfxA"
                     /note="label: precursor"
                     /product="trifolitoxin"
                     /protein_id="AAA26363.1"
                     /transl_table=11
                     /translation="MDNKVAKNVEVKKGSIKATFKAAVLKSKTKVDIGGSRQGCVA"
     gene            794..1915
                     /gene="tfxB"
     CDS             794..1915
                     /codon_start=1
                     /function="required for processing of trifolitoxin"
                     /gene="tfxB"
                     /gene_functions="biosynthetic (rule-based-clusters) LAP:
                     PF00881"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Trifolitoxin_RRE"
                     /gene_kind="biosynthetic"
                     /note="precursor; putative"
                     /protein_id="AAA26364.1"
                     /sec_met_domain="PF00881 (E-value: 3.8e-08, bitscore: 22.5,
                     seeds: 78, tool: rule-based-clusters)"
                     /sec_met_domain="Trifolitoxin_RRE (E-value: 2.2e-62,
                     bitscore: 196.0, seeds: 3, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDFVQRFVIDRSFHLRYYSLDAYLYRAVDQVAWDADITHNRLFWD
                     IWSAFMQPRSLVDAVETLSDYDPDEVAAAIEGMCESGIIEPVGLKDRQFDPLTVELSHV
                     PQAWDYHLVSSRIDWINYLDGKDVKRQDLEQMDKHLSEEAVPSNFHKAANSRPKYDLPS
                     LVPLTAFEFNNSASVAFGHEKAPLPNELSLDIITLLLNYAAAKTDTVNMYATGEHLRKA
                     VPSGGARHPIEFYVVVGDEIAGIEAGVYHYNVRHHRLDAIEIASTSLKALQEASSVLPR
                     SRSKPFGFAFIHTCRFERSMFRYREPRSYRVMQFDLGHIHANEVLAAKILGLDFSETFS
                     VPESIVESVLTLDPFIESAMSAFVVHRHENHHD"
     gene            1908..2978
                     /gene="tfxC"
     CDS             1908..2978
                     /codon_start=1
                     /function="processes trifolitoxin"
                     /gene="tfxC"
                     /note="precursor; putative"
                     /protein_id="AAA26365.1"
                     /transl_table=11
                     /translation="MIELRPLLQLNLEDGIPVLKDLLTADSFSFTDVELLRYIPAIAKN
                     TPAQTRDLAASVADALDVDQTTALAAIEALVELGLLVPSASISSQKAGIQLWVDKGWVD
                     ALILHFASRNLNYNDDPIEFGGLEDIKSYPEPMESKRRKRGTATRLVKPSRELAAAVIL
                     DGLMNRRSFKPFTRKQLSITEVSEILWFGNLYARERAVIAENRDFESPRDIAFDSAFSA
                     LSTFVVTYGQIDWQDGSLPPGVYRYNVVNHELEAIRAGDFKLDMAKLAIGQSRASSGLF
                     TFVICGDLKSYTSRYRHERSYRNLLINTSQLAQFYLTLATINDFNTFLTPAIHDEKMHL
                     FLEAEDDLPLYLVTAG"
     gene            2982..4232
                     /gene="tfxD"
     CDS             2982..4232
                     /codon_start=1
                     /function="processes trifolitoxin peptide"
                     /gene="tfxD"
                     /note="precursor; putative"
                     /protein_id="AAA26366.1"
                     /transl_table=11
                     /translation="MSDENQHGFYRTSFEYASISWRRMIPNVADTIVVTLIGATALQVA
                     SNVLITILTLNIAFLNFCSLICMHNLKRGAKADVFAAIVRAACMMIGVYLALIAVSVAT
                     LEGAPRTQTIAFIALSALRPFVAGWNAYCAEVFFAQGKRQIVRSVITRSSLIYAGVNLL
                     FVGLSHFAGTQNSIISLLIGVYLALFHNALAYARILPTEWRFSRQDLKDVFSLRKLDLV
                     GIGAGLSASFINMLEMGFLALVGWVVAAKFPQIAVFYFPFFTLVELTSGLAIGLGRSVT
                     ERLITPRPFPRLHVLIAVYSTYSLLCFLIYVGLIGVSNRDIFALPLSLAGLALLFLICD
                     GLQLVVRGYTLAKADGGKLTHLSAIAYLASGVILALAAVLGSVQALAIALVLGPLFLAI
                     SIPAVQSRTALNALPNR"
     gene            4213..4971
                     /gene="tfxE"
     CDS             4213..4971
                     /codon_start=1
                     /function="unknown"
                     /gene="tfxE"
                     /gene_functions="biosynthetic (rule-based-clusters) LAP:
                     YcaO"
                     /gene_kind="biosynthetic"
                     /note="precursor; putative"
                     /protein_id="AAA26367.1"
                     /sec_met_domain="YcaO (E-value: 2.3e-10, bitscore: 29.5,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHYRTDKPKVFVTDSGRFVADCQIILLGRKIICTGTDLQYEIAVA
                     KAKSELAERIAFASPDAFNARVTRVARRLMLEATNAFNRESVTLPLSLFVTRPHLCWMR
                     GNNSTGFAAHPRRKAAIEHAVNEVLERGWNARFRRDQQSLLKLATIRRDGSTAWVHESR
                     LRQVSYCLANARVAGHVGWGSAVRRTTEAAVEAATSEAHAMSASGQDYGRGGTGVSELP
                     SQHDQIAFVERQPVRIDDVTHYVIQAVSYS"
     gene            4968..5744
                     /gene="tfxF"
     CDS             4968..5744
                     /codon_start=1
                     /function="processes trifolitoxin peptide"
                     /gene="tfxF"
                     /note="precursor; putative"
                     /protein_id="AAA26368.1"
                     /transl_table=11
                     /translation="MRASKTPILINGSPWLLDFRRRSSREFDWEIAEHLEVPEAYFQAY
                     DPLTTWFEWFSRIGYRDYTDAEAEIERDAEENVRQHQVSVQPDLTLTQRLSSEGSIQLP
                     VPFLKTADQFCILSSLLYAGFGVVETRKFHGDTIFLKNVPSVGARHGIEAYVSLDDGRY
                     YYDCEQHRLFSAGYRGDLRSGQIDIVFRPEVYMWRYQTAACLADVYLDLGHILGTLSMV
                     ASLYDTSITSRSAEAAPVDLINAVHLQRIAVDGFNP"
     gene            5996..6781
                     /gene="tfxG"
     CDS             5996..6781
                     /codon_start=1
                     /function="unknown"
                     /gene="tfxG"
                     /note="precursor; putative"
                     /protein_id="AAA26369.1"
                     /transl_table=11
                     /translation="MNDEICLTGGGRTTVTRRGGVVYREGGPWSSTVISLLRHLEASGF
                     AEAPSVVGTGFDERGRETLSFIEGEFVHPGPWSEEAFPQFGMMLRRLHDATASFKPPEN
                     SMWRDWFGRNLGEGQHVIGHCDTGPWNIVCRSGLPVGLIDWEVAGPVRADIELAQACWL
                     NAQLYDDDIAERVGLGSVTMRAHQVRLLLDGYGLSRKQRGGFVDKLITFAVHDAAEQAK
                     EAAVTPESNDAEPLWAIAWRTRSASWMLHHRQTLEAALA"
ORIGIN
        1 cgcgtaaaag acacgagcag tctccgtaga ccataagaag cttttagagc agccaacgca
       61 tagcagccgc ttttctaaag ctgctagcag cttggtgctt attccttggt agcgtacgat
      121 tggatcgata tacaaaagtg taatctcgcc actaacaaga gccgatccga ctcctcttac
      181 tagtccggca accttagctg taagaaatat tgagtgcggg ttgtcaatcc acatcgatac
      241 gtttgctgcg gtcttgttct ccaaccactc atctatttcg gcagaatttc cgtgatggtc
      301 agccaagcaa agttctgcga ttgatcgccg caatacacgg gcgcagtcgg cggcatctat
      361 cgccgaagcg tcaccaattt ccgcagcgag gttttctcgc tgcataattt ttttctttcc
      421 tgaatcgatc attagttgtg ttttttgttg ctctcgacgt atttgcaacc gtttgattcg
      481 attgcgtatt tgtcaaaata ctccatatga ttgcattttt taaaagacaa gataggctca
      541 catttgtcag caaatgactg ctggcaaacc ccaatcgcta aatgaggtgt tgttgcatgg
      601 ataacaaggt tgcgaagaat gtcgaagtga agaagggctc catcaaggcg accttcaagg
      661 ctgctgttct gaagtcgaag acgaaggtcg acatcggagg tagccgtcag ggctgcgtcg
      721 cttaagtgaa catccggcgg gtgcggcaaa cgtacccgcc acttatgccc tcgctttcaa
      781 cgggatgttt cgcatggact tcgtccaacg attcgtaatc gaccgctctt tccacctccg
      841 ctactacagc ctcgacgcct atctatatcg cgcagttgac caggtcgcct gggacgcaga
      901 catcactcac aatcgcctat tttgggacat ttggtcagca ttcatgcagc cgagaagtct
      961 ggtagacgct gttgagacgc tatccgatta cgatcccgac gaagtggccg cagcaatcga
     1021 aggcatgtgc gagtcgggca tcatcgaacc ggtgggcttg aaagaccgcc aatttgatcc
     1081 tttgacggta gagctgtcac atgtgccaca ggcatgggat tatcacctgg tctcaagtcg
     1141 catcgactgg atcaattatc tggatgggaa ggacgttaaa cgccaggacc ttgaacaaat
     1201 ggacaagcat ttgtcggagg aggctgttcc gtcgaatttt cacaaggccg ccaactctcg
     1261 accgaaatat gatttgccaa gtttagtgcc gctgacagcg ttcgaattca ataactcggc
     1321 gtccgtcgca ttcggtcatg agaaggcacc gcttccgaac gaactgtcgc tcgatataat
     1381 cacattgctc ctcaactatg cggccgcaaa gacggatacc gtcaacatgt atgccactgg
     1441 cgagcatctg cgaaaggccg tcccatccgg aggagcgcga caccccatcg aattctacgt
     1501 ggttgtcggc gatgagattg caggtatcga agctggcgta tatcactaca atgttcgcca
     1561 tcatcggctc gatgctatcg aaatagcgtc cacctcattg aaagcactgc aagaggcaag
     1621 ctcagtgctg ccccgatcac ggtcaaaacc gttcggcttt gctttcattc atacatgtcg
     1681 gttcgagcga agtatgtttc ggtaccgcga accgcgaagc taccgtgtga tgcagtttga
     1741 tcttgggcat atccatgcca acgaggtttt ggctgccaaa atcctcggcc tcgatttcag
     1801 tgaaaccttt tctgtgccgg aaagcatagt tgagagcgtc ttgacgctcg atccgttcat
     1861 cgagtccgcg atgtcagcct ttgtcgtcca cagacacgag aaccaccatg attgaactgc
     1921 gcccgcttct ccaactgaat cttgaagatg gaatcccggt cctcaaagac ctcctgaccg
     1981 ccgacagctt ttccttcacc gatgttgaac tcttgcggta cattccagcc attgccaaga
     2041 acacccccgc ccagactcgg gatttggctg cctctgttgc tgatgcgctg gatgttgacc
     2101 aaaccaccgc gctcgcagcc atcgaagcat tggttgagct tggtcttttg gtgccatccg
     2161 cgtcgatctc ctcgcagaag gcagggatcc agttgtgggt ggataaggga tgggtggacg
     2221 cactgatcct gcatttcgcg agcagaaatc tcaattataa tgacgatcca attgaatttg
     2281 gcgggttgga ggatatcaaa agctatcccg aaccgatgga atcgaagcgt aggaaacgcg
     2341 gcaccgccac gcgattggtc aagccgtccc gggagctggc agctgcagtc atactggacg
     2401 ggctcatgaa caggcgctcg ttcaaaccct tcacacgcaa acaactgtcg atcaccgagg
     2461 tcagcgagat actttggttt gggaacctct atgcgcgaga acgcgcggtc atcgctgaaa
     2521 atcgtgactt cgagtctcct cgcgatatag ctttcgacag cgccttctca gcgttgtcca
     2581 cctttgttgt cacatatgga cagatcgatt ggcaggatgg ctcgttgcca cccggagtct
     2641 atcgttacaa tgtcgtcaat cacgaacttg aagcaatcag agccggtgat ttcaagctgg
     2701 acatggcaaa actcgctatc ggtcagagtc gggcttcgag cgggctattc acgtttgtga
     2761 tctgcggcga tttgaagtca tacacatcgc ggtacaggca cgagcggagc taccgcaatc
     2821 tgctgatcaa cacctcacag ctcgcccaat tctatttgac cctcgcaacg atcaacgact
     2881 tcaacacctt tctcacgccc gccatccacg atgagaaaat gcatctgttt cttgaagcgg
     2941 aggacgacct cccgctttat ctcgtcacgg caggctagag catgagcgac gaaaaccagc
     3001 atgggttcta tcggacttcg ttcgaatacg catcgatcag ttggcggaga atgattccca
     3061 atgtggctga cactatcgtc gtcacgctca tcggcgctac tgcacttcag gtggcgtcaa
     3121 atgttctgat cacgatactg accctcaata tcgcttttct gaacttttgc tcgcttatct
     3181 gcatgcacaa tctgaaaaga ggggcaaagg ccgacgtatt tgctgcaatc gtccgcgctg
     3241 cttgcatgat gatcggggtc tacctggcgc ttatcgcggt ctccgtcgcc accctcgaag
     3301 gtgcaccgcg tacccaaacc attgctttca tagcactgtc tgcgctccgg ccgtttgtgg
     3361 ctggatggaa tgcttactgt gcggaggttt ttttcgccca gggaaaacga caaattgtgc
     3421 gaagcgtcat cacgagatcg tcgctgatct atgcaggagt taatctgctc tttgtcgggc
     3481 tgtcgcattt cgctggcact caaaattcga tcatatcgct tctcatcggc gtatatcttg
     3541 ctctcttcca caacgccctg gcctacgcca gaatcctgcc gaccgaatgg aggttcagtc
     3601 gccaggattt gaaggatgtc ttctcacttc ggaagcttga tctggtcgga atcggggcag
     3661 ggctttctgc gtcttttatc aacatgctcg aaatggggtt tcttgcatta gttgggtggg
     3721 tggtggcagc aaagtttccg caaatcgcgg ttttttattt cccgtttttc actttggtgg
     3781 aattgacgag cggacttgcg attgggcttg gacgctcagt caccgaacgt ttgattacgc
     3841 cgcgcccgtt tccccggctg cacgtcttga tcgccgttta cagcacgtat tcgttgctct
     3901 gcttcttgat ctacgttgga ttaataggtg tgagcaatcg ggacatattt gctctcccgc
     3961 tgtcgcttgc cggattggcg ctacttttcc tgatctgcga cgggctgcag cttgtggttc
     4021 ggggatatac gctcgccaaa gctgacggag gcaagctcac gcatctcagc gccattgcat
     4081 acctagcctc tggagtgatc ctcgcgctgg cggccgtctt gggctcggtt caagcgttgg
     4141 ccatcgcttt ggtcttggga ccgctgttcc ttgcaatctc cattcccgcc gttcaaagtc
     4201 gaactgccct aaatgcacta ccgaacagat aaaccgaaag tattcgtaac cgactccggc
     4261 aggtttgttg ctgactgcca gataattctg cttggtcgca agatcatatg tacgggaacc
     4321 gatcttcagt acgagattgc ggttgcaaaa gcaaagtctg agcttgcgga gaggatcgcg
     4381 tttgcatcac cagacgcctt caatgcgcga gtgacgcggg ttgcaaggcg tctcatgctc
     4441 gaagctacca atgccttcaa cagagaatcc gtcaccctgc cgttgagctt gttcgtaacg
     4501 cggccacatc tgtgctggat gcgcggcaac aactccacag gatttgccgc acatccccgg
     4561 cgcaaggcag caattgaaca cgcggtcaat gaggttttag agcgcggctg gaacgctcgg
     4621 tttcgacgag atcagcagtc tcttcttaag ttggccacga tccgacgaga cggttcaaca
     4681 gcttgggtac acgaatcgag gctccgccag gtttcctact gcttggccaa cgcccgtgtt
     4741 gcgggacatg tcggttgggg ttccgcagtt cgcagaacga ccgaagcggc agtggaagct
     4801 gcaactagtg aagcccatgc gatgagtgcg tcggggcagg attacggtcg cggcggaacc
     4861 ggcgtgtcgg agcttccctc gcaacacgac caaatcgcgt ttgtcgagag gcagccggtg
     4921 cgcatcgatg acgtcactca ttacgtgata caggcggtga gttattcatg agagcaagca
     4981 aaacaccgat cttgataaac ggctctccgt ggttgttaga tttccgtcgg cggtcaagcc
     5041 gagaattcga ttgggaaatt gccgaacatc tagaggtgcc cgaagcatat tttcaggcgt
     5101 atgacccgct aacaacttgg ttcgagtggt tttctcggat cggctatcga gattacaccg
     5161 atgctgaggc cgaaattgag cgagatgccg aggaaaatgt acggcagcac caagtttccg
     5221 ttcaacccga tctcacgctg acccagcgcc tatcgagcga aggctcgatc cagcttccag
     5281 ttccgttcct aaaaacggcc gatcaatttt gtatcttgtc gtcgctcctg tacgccggtt
     5341 ttggagtggt tgagacgcgg aaattccacg gtgacacgat cttcctaaaa aacgtacctt
     5401 cggttggagc gcgtcacggc attgaggctt atgtttccct ggatgacggg cgctattatt
     5461 acgactgtga gcagcatcgg ttgttttccg caggctatcg gggtgatcta cggagcggtc
     5521 agatcgatat cgtatttcgg cctgaggtat acatgtggcg ttatcaaacc gctgcctgtc
     5581 ttgccgatgt ctacctcgac cttggccaca tattaggtac tctatcgatg gtggcgtccc
     5641 tctatgacac gtctatcacg agccgctctg cagaagccgc tcctgtagac ttgatcaatg
     5701 cggtgcatct ccagcgaata gccgttgatg gatttaatcc ataggcgcag gacgggaatg
     5761 cctgcgaact gaagaaggcc gacgatccgt ttttctcttg atgaacgccg tcggccagtc
     5821 gtccgttttg ggccgtaagc gctgacccag cggcggcaac agcgaccgtg tctttatggc
     5881 ggcttgccaa cgacaggagc gaggcccttg aggtgcagaa atcgttgccg gggggcgaag
     5941 gctgaaaggt aaacgcgccg cttgtggtgc tactaatgga atccaggtgg gtgccatgaa
     6001 tgatgagatt tgcctgacag gtggcggacg aacgactgtc acgcggcgcg gcggagtcgt
     6061 gtatcgcgaa ggcggcccgt ggtcatcaac cgtcatttcg ctcctgcggc atctggaagc
     6121 ctctggcttc gctgaagctc cttccgttgt cggcaccggt ttcgatgagc gcggccggga
     6181 gacattatcg tttatcgagg gtgagtttgt tcacccaggc ccttggtcgg aggaggcttt
     6241 tccgcaattt ggaatgatgt tgcggcgact gcacgatgcc accgcctcgt tcaaacctcc
     6301 cgaaaactcg atgtggcgcg attggttcgg gcgtaacctc ggtgagggtc aacacgtaat
     6361 aggacactgc gacacaggcc catggaacat tgtttgccgg tcaggattgc ctgtcgggtt
     6421 gatagattgg gaggtggctg ggcctgtcag ggcggatatc gaattggccc aggcttgttg
     6481 gctgaatgcc cagctctacg atgacgacat tgcggagagg gtcggattag gctctgtgac
     6541 catgagagcg catcaagttc gcctgctgct tgacggctat ggtctgtctc ggaagcaacg
     6601 cggcggcttc gtcgacaagc taatcacgtt cgcagttcac gatgcggccg agcaggcgaa
     6661 agaggcggct gtcacgccag agtcgaacga tgcggaaccg ctatgggcaa ttgcctggcg
     6721 cactagaagt gcctcctgga tgctccatca tcggcaaaca ctggaagcag cgctggcata
     6781 gtaggcagcg accgcgccat aagtcgtggg acgaagctgc ggactggggt tgcgaggtta
     6841 agttcagcaa gcaaggggga gacactatgg aagcttcgtt caggccgttc gtccgcttta
     6901 tccacgaaaa acagatgcaa cttctcgaag agactgcaaa aagtccgaaa ggctcctggc
     6961 tgtgtgacgc gctcggtgat ccggaactat tcttcgcctt gagagacgag cgtatcgacg
     7021 tctactatcg cggacgggcc atctattcca tcgagttcag cggtggcaag gtgacaccac
     7081 ggacccatgt gaagtacctg gttctcgacg accgtgaccc ttacatcaag atgcagaacg
     7141 cg
//
