LOCUS       BGC0000568              5145 bp    DNA     linear   BCT 15-JUN-2009
DEFINITION  Escherichia coli microcin B17 operon (mcbGFEDCBA genes), strain
            L1000.
ACCESSION   BGC0000568
VERSION     BGC0000568
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
COMMENT     ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2024-10-17 13:51:16
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5145
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     gene            complement(1..564)
                     /gene="mcbG"
     CDS             complement(1..564)
                     /codon_start=1
                     /db_xref="InterPro:IPR001646"
                     /db_xref="UniProtKB/TrEMBL:B7VF08"
                     /function="antibiotic resistance"
                     /gene="mcbG"
                     /operon="microcin B17"
                     /product="protein mcbG"
                     /protein_id="CAT00693.1"
                     /transl_table=11
                     /translation="MDIIEKRITKRHLSESELSGVNYYNCIFERIQLDNFNFRDCEFEK
                     CRFVNCSIKNLKLNFFKLIDCEFKDCLLQGVNAADIMFPCTFSLVNCDLRFVDFISLRL
                     QKSIFLSSRFRDCLFEETDLRKSDFTGSEFNNTEFRHSDLSHCDFSMTEGLDINPEINR
                     ILSIKIPQEAGLKILKRMGVVVGG"
     source          1..5145
                     /db_xref="taxon:562"
                     /mol_type="genomic DNA"
                     /organism="Escherichia coli"
                     /strain="L1000"
     operon          1..5145
                     /operon="microcin B17"
     subregion       1..5145
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="ribosomal"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..5145
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(568..1299)
                     /gene="mcbF"
     CDS             complement(568..1299)
                     /codon_start=1
                     /db_xref="GOA:B7VF09"
                     /db_xref="InterPro:IPR003439"
                     /db_xref="InterPro:IPR003593"
                     /db_xref="UniProtKB/TrEMBL:B7VF09"
                     /function="antibiotic resistance"
                     /gene="mcbF"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 95.1; E-value:
                     9.3e-29)"
                     /gene_kind="transport"
                     /operon="microcin B17"
                     /product="protein mcbF"
                     /protein_id="CAT00694.1"
                     /transl_table=11
                     /translation="MTIPLLEINSLSFSYKVNLPPVFNNLSLKIEQGELIGLLGENGAG
                     KTTLFNLIRGGVSNYEGTLKRNFSGGELVSLPQVINLSGTLRNEEVLDLICCFNKLTKK
                     QAWTELNHKWNDNFFIRYDKIRRKRTYTVSYGEKRWLIISLMLTLCKNARLFLLDEPTV
                     GIDIQYRMMLWELINKITADGKTVFFSTHIFDELTRDKIPFYMLSKNSINRYSDMSDFI
                     QSNNETTPEKAFIKEVMGTGD"
     gene            complement(1296..2021)
                     /gene="mcbE"
     CDS             complement(1296..2021)
                     /codon_start=1
                     /db_xref="UniProtKB/TrEMBL:B7VF10"
                     /function="antibiotic resistance"
                     /gene="mcbE"
                     /operon="microcin B17"
                     /product="protein mcbE"
                     /protein_id="CAT00695.1"
                     /transl_table=11
                     /translation="MVTLKMAIIFLMEQIRTPFSLIWTIMSPTVLFFFLHFNEIELHYG
                     DTAWLGKQISWFVGYISFSVVLFNYCLYLVGRRESGFIATFVHNMDGRLLFIRSQLIAS
                     LIMSILYVFFFILVVLTGFQASPDYQIVMIILKSIYINAFMMVSLTFMASFRVTFQTAS
                     TIYSVLITVCMVSGIVSLKYNEGIVYWINQVNPIAIYSTILQSDQELSLMTIFFYSIML
                     IISIISALTFKTEPVWSSQ"
     gene            complement(2031..3221)
                     /gene="mcbD"
     CDS             complement(2031..3221)
                     /codon_start=1
                     /db_xref="InterPro:IPR003776"
                     /db_xref="UniProtKB/TrEMBL:B7VF11"
                     /function="antibiotic resistance"
                     /gene="mcbD"
                     /gene_functions="biosynthetic (rule-based-clusters) LAP:
                     YcaO"
                     /gene_kind="biosynthetic"
                     /operon="microcin B17"
                     /product="protein mcbD"
                     /protein_id="CAT00696.1"
                     /sec_met_domain="YcaO (E-value: 3.1e-17, bitscore: 52.1,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINVYSNLMSAWPATMAMSPKLNRNMPTFSQIWDYERITPASAAG
                     ETLKSIQGAIGEYFERRHFFNEIVTGGQKTLYEMMPPSAAKAFTEAFFQISSLTRDEII
                     THKFKTVRTFNLFSLEQQEIPAVIIALDNITAADDLKFYPDRDTCGCSFHGSLNDAIEG
                     SLCEFMERQSLLLYWLQGKANTEISSEIVTGINHIDEILLALRSEGDIRIFDITLPGAP
                     GHAVLTLYGTKNKISRIKYSTGLSYANSLKKALCKSVVELWQSYICLHNFLIGGYTDDD
                     IIDSYQRHFMSCNKYESFTDLCENTVLLSDDVKLTLEENITSDTNLLNYLQQISDNIFV
                     YYARERVSNSLVWYTKIVSPDFFLHMNNSGAININNKIYHTGDGIKVRESKMVPFP"
     gene            complement(3202..4020)
                     /gene="mcbC"
     CDS             complement(3202..4020)
                     /codon_start=1
                     /db_xref="GOA:B7VF12"
                     /db_xref="InterPro:IPR000415"
                     /db_xref="InterPro:IPR020051"
                     /db_xref="UniProtKB/TrEMBL:B7VF12"
                     /function="antibiotic resistance"
                     /gene="mcbC"
                     /gene_functions="biosynthetic (rule-based-clusters) LAP:
                     PF00881"
                     /gene_kind="biosynthetic"
                     /operon="microcin B17"
                     /product="protein mcbC"
                     /protein_id="CAT00697.1"
                     /sec_met_domain="PF00881 (E-value: 7.5e-11, bitscore: 31.3,
                     seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKHELSLVEVTHYTDPEVLAIVKDFHVRGNFASLPEFAERTFVS
                     AVPLAHLEKFENKEVLFRPGFSSVINISSSHNFSRERLPSGINFCDKNKLSIRTIEKLL
                     VNAFSSPDPGSVRRPYPSGGALYPIEVFLCRLSENTENWQAGTNVYHYLPLSQALEPVA
                     TCNTQSLYRSLSGGDSERLGKPHFALVYCIIFEKALFKYRYRGYRMALMETGSMYQNAV
                     LVADQIGLKNRVWAGYTDSYVAKTMNLDQRTVAPLIVQFFGDVNDDKCLQ"
     gene            complement(4022..4909)
                     /gene="mcbB"
     CDS             complement(4022..4909)
                     /codon_start=1
                     /db_xref="UniProtKB/TrEMBL:B7VF13"
                     /function="antibiotic resistance"
                     /gene="mcbB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR04424"
                     /gene_kind="biosynthetic-additional"
                     /operon="microcin B17"
                     /product="protein mcbB"
                     /protein_id="CAT00698.1"
                     /sec_met_domain="TIGR04424 (E-value: 2.9e-89, bitscore:
                     288.3, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVLPDIKKGKDMINILPFEIISRNTKTLLITYISSVDITHEGMKK
                     VLESLRSKQGIISEYLLDKLLDESLIDKDKGKEFLITTGVINKTKTSPLWVNSVIISDV
                     PHLFSNAREQWKSDGVFVSHIIDIKDNNINVSDSTLIWLHLENYHSDIVKRIYSKFESN
                     PGVAFIQSYYLKESFRIDGVYSPDLGTPCHFCHIDRWLSREEKSFRRNEMSWANLLQLL
                     KKYQMTLPALALGESERGFSYHLIKRRLQELTGTSLVKSHVDNFMSSVSADLITCILCK
                     EPVIHWQACSCLER"
     gene            complement(4936..5145)
                     /gene="mcbA"
     CDS             complement(4936..5145)
                     /codon_start=1
                     /db_xref="UniProtKB/TrEMBL:B7VF14"
                     /function="antibiotic resistance"
                     /gene="mcbA"
                     /operon="microcin B17"
                     /product="protein mcbA"
                     /protein_id="CAT00699.1"
                     /transl_table=11
                     /translation="MELKASEFGVVLSVDALKLSRQSPLGVGIGGGGGGGGGGSCGGQG
                     GGCGGCSNGCSGGNGGSGGSGSHI"
ORIGIN
        1 tcatccccct acaaccactc ccattcgttt aagtattttt aaacctgctt cctgtggtat
       61 ctttatagat aagatcctgt ttatctcagg atttatatcg agaccttctg tcattgaaaa
      121 atcacagtga gacaaatcag aatgtctgaa ctctgtatta ttaaactccg aaccagtaaa
      181 atcagattta cgaaggtctg tttcctcaaa taaacaatca cggaagcgac tggataaaaa
      241 aatagatttc tgtaaccgta aactaataaa atcaacaaat ctcaaatcac aatttacaag
      301 agaaaaagta cagggaaaca taatgtctgc tgcatttact ccttgtaata aacaatcttt
      361 gaattcacag tcaataagtt taaaaaaatt aagttttaaa ttctttattg agcagtttac
      421 gaagcgacat ttttcgaact cacaatcgcg aaagttaaag ttgtcaagct gtattcgttc
      481 aaatatacaa ttgtaataat ttacacctga taattcactt tcactgaggt gtcgttttgt
      541 gattcttttt tctattatat ccatgtgtca gtctcctgtt cccataactt ctttaataaa
      601 tgccttttct ggagttgttt cattattgga ttgaataaaa tcactcatat cagaataacg
      661 atttatacta ttttttgaaa gcatatagaa ggggatttta tctcttgtta attcatcaaa
      721 aatatgagtt gagaaaaata cagttttccc gtcagcggtg atcttattta tcagttccca
      781 taacatcatt ctgtactgaa tatcaatgcc tacagttggt tcatccagta aaaataaacg
      841 ggcatttttg caaagcgtta gcatcaaaga tatgataagc caacgcttct ctccataact
      901 taccgtgtaa gtccttttac gtcttatttt atcatagcga atgaaaaaat tatcattcca
      961 tttatgatta agctctgtcc atgcctgttt tttagttaac ttattgaagc agcaaatcaa
     1021 atcaagaacc tcctcattcc ttagtgtacc tgaaagattt atgacttgag gtagacttac
     1081 taattctcca ccagagaaat ttcgcttgag ggtcccttca taattactga ctccgcccct
     1141 gataaggtta aaaagagtgg tcttcccggc tccattttct ccaagcagac ctatcaactc
     1201 tccctgctca atcttaagcg acagattatt gaaaacaggg ggtaaattta ctttatatga
     1261 aaagctcaga gagttgattt ctaaaagagg tatggtcatt gagaactcca gactggctca
     1321 gttttgaagg ttagtgctga aataatactt atgattaaca ttatactata gaaaaatatc
     1381 gtcatcagag acaactcctg atctgactgg agtattgtag aatagatggc gataggatta
     1441 acctgattta tccagtaaac gatcccctca ttatatttta atgatacaat tcctgatacc
     1501 atacagacag ttattaatac agaatagata gtgctggctg tttggaaggt gactctgaac
     1561 gatgccatga aagtgagtga aaccatcata aatgcattta tgtatatcga ttttaaaata
     1621 atcattacta tttgataatc tggacttgcc tggaatcctg ttagtacgac taaaatgaaa
     1681 aaaaacacat acaaaataga catgataagt gaggctatta attgagaacg aataaataat
     1741 agtcgaccat ccatattatg cacgaaggta gcaataaaac cactttctct tcttccgacc
     1801 agatacagac agtaattaaa cagaacaaca gaaaaagaaa tgtagcctac aaaccatgat
     1861 atttgttttc cgagccatgc tgtatcacca taatgaagtt caatttcatt gaaatgaagg
     1921 aaaaagaaca acactgtagg tgacataatt gtccatatta agctgaaagg ggttctgatt
     1981 tgttccatca gaaaaattat cgccattttt aatgtaacca tttgttatcc ttatgggaat
     2041 ggtaccatct ttgattctct gactttaata ccgtccccgg tatggtaaat tttattatta
     2101 atgtttattg cacctgagtt attcatatga aggaaaaaat cagggcttac tatttttgtg
     2161 taccagacaa ggctgttact tactctttcc ctggcatagt aaacaaaaat attatcagaa
     2221 atttgttgaa gatagtttaa taaatttgtg tctgacgtaa tattttcctc aagcgttaac
     2281 ttgacatcat cagacagtag taccgtattt tcacacaaat ccgtaaacga ctcgtacttg
     2341 ttgcatgaca taaagtgacg ctggtaacta tcaataatgt catcatcagt ataaccgcca
     2401 ataagaaagt tgtgcaggca tatatacgat tgccacaatt ccactacgga tttacaaagt
     2461 gcttttttca gactattagc ataggataat ccggtactgt attttattcg actgattttg
     2521 ttttttgtgc catacagggt tagtactgcg tgtccaggag ctccgggcag ggtgatatcg
     2581 aaaatcctga tatctccttc tgacctgaga gccagtaaaa tctcatctat atgatttatg
     2641 cctgttacta tttcactgga tatttcagta ttggcttttc cctgtaacca gtaaagaagg
     2701 agggactgtc tctccataaa ttcacacaag gaaccttcta tggcatcgtt caaactacca
     2761 tgaaagctac atccgcatgt atctctgtca ggataaaatt tcagatcatc tgcagcggtt
     2821 atattgtcga gtgcaattat gactgcaggt atttcttgtt gttcaaggct aaacagatta
     2881 aaggttctga ccgttttaaa tttatgggtt atgatttcat cgcgggtcag tgatgagatc
     2941 tgaaaaaatg cttcggtaaa agcctttgca gcagatggag gcatcatctc atataatgtt
     3001 ttctgaccac cggtgactat ctcattaaaa aaatgacggc gttcaaaata ttcacctatt
     3061 gccccctgaa ttgacttcag agtttcaccg gccgcgctgg ctggtgtaat acgctcatag
     3121 tcccatatct gagaaaacgt tggcatattt ctgttcagtt ttggactcat ggccattgtg
     3181 gccggccatg cggacataag gttactgtag acatttatca tcgtttacat ctccaaaaaa
     3241 ctgaacgatc agtggcgcta cagtcctctg atccagattc attgtttttg ctacgtatga
     3301 atcggtatat cccgcccata cccggttttt cagtcctatt tgatctgcaa ccaatactgc
     3361 gttctgatac atcgaacctg tttccattaa ggccatccgg tatcctctgt agcgatattt
     3421 gaacaaagct ttttcaaaaa taatgcaata gacgagagca aaatggggtt taccaagacg
     3481 ttccgaatcc ccaccggaca ggcttcggta gagtgactga gtattacatg tagcaacagg
     3541 ttccagtgcc tgacttagcg gcaggtagtg ataaacatta gttcctgcct gccagttttc
     3601 tgtattttca gataatctgc ataaaaaaac ttcaatcggg tacaatgccc ccccagaagg
     3661 ataaggtctt cttacagagc caggatcagg tgagctgaat gcattgacta ataacttttc
     3721 aatagtacga atggaaagtt tatttttgtc gcaaaagttt attcctgatg ggagcctttc
     3781 acgactaaaa ttatgtgatg aggatatgtt tattacggag ctgaaacctg gcctgaagag
     3841 aacttcttta ttttcaaatt tctccagatg ggcaagaggt accgcggaca cgaaagttcg
     3901 ttcagcaaat tcggggaggg aagcaaagtt acctctgaca tgaaaatctt taacaatggc
     3961 cagaacttca ggatctgtgt aatgcgttac ttccactaaa gagagttcgt gttttgacat
     4021 gttatctctc cagacagctg caagcctgcc agtgaattac cggctcttta cataaaatac
     4081 aggtgattaa atcagcgcta acagatgaca taaagttatc gacatgactt ttaaccagtg
     4141 aagtacctgt caactcctga agtcgccgtt ttattaaatg atagctgaat cctctttctg
     4201 attcgcctaa agccaatgct ggtagtgtca tttggtattt tttcagtaac tgaagtaagt
     4261 ttgcccagga catttcgtta cgtctgaagc ttttttcttc cctgctcagc catctatcta
     4321 tatgacaaaa atggcaggga gtaccaagat caggtgaata tactccatct atcctgaatg
     4381 actcctttag gtagtagctt tggatgaagg caacaccagg gttactttca aattttgaat
     4441 agattctttt tacaatgtct gaatgatagt tttcgagatg taaccagatt agtgttgaat
     4501 cgctcacatt aatattatta tcttttatat caatgatatg agaaacaaaa acaccatcag
     4561 atttccattg ttcccgggca ttactgaaaa gatgtggaac atcgcttatt atgactgagt
     4621 ttacccagag aggtgatgtt ttcgttttat ttatcacgcc tgtggtaata agaaactctt
     4681 tgcccttgtc tttatcaata agcgattcgt ccagtagttt atcaaggagg tattcagaaa
     4741 taataccctg tttggatcgt aggctttcga gtactttttt cattccctca tgtgtaatgt
     4801 ctactgaaga aatgtaggta ataagcaggg tctttgtatt tctggaaatt atctcaaacg
     4861 gcagaatatt gatcatatct tttccttttt taatatcagg gagcaccatg ctccctgaac
     4921 ggttaattca acgtatcaga tatgtgaacc acttccgccg ctgccaccgt ttccaccact
     4981 acaaccgttg ctgcaaccac cacaaccgcc accttgacca ccgcagctac cgccgccgcc
     5041 gccgccgcca ccaccaccaa tgccaacacc taatggagac tggcgtgata atttaagagc
     5101 atcaacggac aaaactacac caaattcact cgcttttaat tccat
//
