LOCUS       BGC0000569              9892 bp    DNA     linear   BCT 28-SEP-2010
DEFINITION  Bacillus amyloliquefaciens ptn gene cluster for plantathiazolicin,
            strain FZB42.
ACCESSION   BGC0000569
VERSION     BGC0000569
KEYWORDS    .
SOURCE      Bacillus velezensis FZB42
  ORGANISM  Bacillus velezensis FZB42
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus amyloliquefaciens group.
COMMENT     ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2024-10-17 13:51:21
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 9892
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     gene            1..594
                     /gene="ptnE"
                     /operon="ptnEFGHI"
     CDS             1..594
                     /codon_start=1
                     /db_xref="UniProtKB/TrEMBL:D3VML0"
                     /function="immunity against plantathiazolicin"
                     /gene="ptnE"
                     /operon="ptnEFGHI"
                     /product="PtnE protein"
                     /protein_id="CBJ61630.1"
                     /transl_table=11
                     /translation="MPNQNFRESEIAENLSHIKVLTNYKTEYSRQTSPYFIIWGCIWII
                     AYSVSLCKVPSVVLACVWLILAVLGWILTLKTYLKQKQFDPMPTFLKNQLKYAWIGFIL
                     MVCIFVFLILMGLLPRTGEFLSFLIVLMVSIMYIFLGIVLTKEIFVMGFWLSILGMLTF
                     CLLPNAMNIVFAFIGGGSLLLTGMILKRKGQGNE"
     operon          1..3321
                     /function="immunity and transport plantathiazolicin"
                     /operon="ptnEFGHI"
     source          1..9892
                     /db_xref="taxon:326423"
                     /mol_type="genomic DNA"
                     /organism="Bacillus velezensis FZB42"
                     /strain="FZB42"
                     /type_material="type strain of Bacillus amyloliquefaciens
                     subsp. plantarum"
     misc_feature    1..9892
                     /note="ptn gene cluster for plantathiazolicin"
     subregion       1..9892
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="ribosomal"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..9892
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            587..898
                     /gene="ptnF"
                     /operon="ptnEFGHI"
     CDS             587..898
                     /codon_start=1
                     /db_xref="GOA:D3VML1"
                     /db_xref="InterPro:IPR001845"
                     /db_xref="InterPro:IPR011991"
                     /db_xref="UniProtKB/TrEMBL:D3VML1"
                     /function="repressor"
                     /gene="ptnF"
                     /operon="ptnEFGHI"
                     /product="PtnF protein"
                     /protein_id="CBJ61631.1"
                     /transl_table=11
                     /translation="MSNVNNINDLIHAKVRLGIMSLLMTYDQCDFTFLKKSLDITDGNL
                     GSHLKKLEDAAYIELTKKFVSKKPKTIVQITKEGKAAYKEYINTIESIFKINNSGGMV"
     gene            899..1789
                     /gene="ptnG"
                     /operon="ptnEFGHI"
     CDS             899..1789
                     /codon_start=1
                     /db_xref="GOA:D3VML2"
                     /db_xref="InterPro:IPR003439"
                     /db_xref="InterPro:IPR003593"
                     /db_xref="InterPro:IPR017871"
                     /db_xref="UniProtKB/TrEMBL:D3VML2"
                     /function="ABC transporter"
                     /gene="ptnG"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 186.7; E-value:
                     8.7e-57)"
                     /gene_kind="transport"
                     /operon="ptnEFGHI"
                     /product="PtnG protein"
                     /protein_id="CBJ61632.1"
                     /transl_table=11
                     /translation="MLQVEHISKRYGTTLALDNVALTIPKGVCYGLVGPNGSGKSTLIK
                     IISRIIREYDGKVEFVGNENGRLGYVPQDISLEEKLTAKTNMEFFGKVHGLNGLKLKQT
                     TKKLLEDIGLSERTNDVVENFSGGMKRRLNIGCALMHDPDLIILDEPTVGVDPQSRRYI
                     FNIVNRLKKAGKTIIYVSHYMEEIETLCDYVAFIDRGQIIDEGEINELLKRHKESSIMF
                     NSSLSHELIKSHGFEFSVVNSGLIIKTKKPLETMERLISVSKENNIVPHQLFLYKPKLE
                     DVFFKLTGTDLRDEE"
     gene            1804..2544
                     /gene="ptnH"
                     /operon="ptnEFGHI"
     CDS             1804..2544
                     /codon_start=1
                     /db_xref="GOA:D3VML3"
                     /db_xref="InterPro:IPR000412"
                     /db_xref="InterPro:IPR013525"
                     /db_xref="UniProtKB/TrEMBL:D3VML3"
                     /function="ABC transporter"
                     /gene="ptnH"
                     /operon="ptnEFGHI"
                     /product="PtnH protein"
                     /protein_id="CBJ61633.1"
                     /transl_table=11
                     /translation="MLPLAQIETLKQLKDKSLFFWTIILPIVSIILLIELFTTDSTNKT
                     LVASQSVTGMSVFFSTFIIISIVISFVKDREKGIVSRLASTPLKPYNFFLGKSIPFLVI
                     TFMQILLLSVLGIFLYDIEINNFPAYLWVVVLISLLVTSWGVAISVFAKTENTGLVMTN
                     VIALGTAVVCGLWTPYDQLPSYLQTIGMLFPQYWAHQGLIQSVSTISGGESLIWVTLVV
                     LGYTILGFIVSLIGYRHYIKMARN"
     gene            2599..3321
                     /gene="ptnI"
                     /operon="ptnEFGHI"
     CDS             2599..3321
                     /codon_start=1
                     /db_xref="UniProtKB/TrEMBL:D3VML4"
                     /gene="ptnI"
                     /operon="ptnEFGHI"
                     /product="PtnI protein"
                     /protein_id="CBJ61634.1"
                     /transl_table=11
                     /translation="MISEKLKLYVEALFKKYERNSLLSRKKTELLTKLHDRSISLEAEG
                     MTKLDIEKLLIREIESKSLAVDTSDLNIDKSNVLKLKKKTFINKNLQKTEDFEPVNAEY
                     YLSDFKSATLQNIDVEHSVFKNCYFKNFSCKDSAIIESTFKKSDLSQSNYDTCKLEYML
                     YTGCHLPKVNFTDTSILHTFFKNCYLKKVSFTNCILINIRFESCDIANVKITGGKMDKT
                     TYRILQEEGTSLHSVELI"
     gene            complement(3383..3508)
                     /gene="ptnA"
     CDS             complement(3383..3508)
                     /codon_start=1
                     /db_xref="UniProtKB/TrEMBL:D3VML5"
                     /function="propeptide scaffold for plantathiazolicin"
                     /gene="ptnA"
                     /product="PtnA protein"
                     /protein_id="CBJ61635.1"
                     /transl_table=11
                     /translation="MTQIKVPTALIASVHGEGQHLFEPMAARCTCTTIISSSSTF"
     regulatory      complement(3517..3522)
                     /regulatory_class="ribosome_binding_site"
     regulatory      4473..4478
                     /regulatory_class="ribosome_binding_site"
     gene            4487..5191
                     /gene="ptnJ"
                     /operon="ptnJDCBKL"
     CDS             4487..5191
                     /codon_start=1
                     /db_xref="UniProtKB/TrEMBL:D3VML6"
                     /function="essential for plantathiazolicin maturation"
                     /gene="ptnJ"
                     /operon="ptnJDCBKL"
                     /product="PtnJ protein"
                     /protein_id="CBJ61636.1"
                     /transl_table=11
                     /translation="MKIHYMGVREPLKQILEDKAAELHDYPLQIVDLGGSIKYESDSFI
                     NSFDLDAETIILSNRRYNLNDELGYVETELLQKYKKSILYRTSPLDIGIELYRGWAAAT
                     GILLTAFSPVGVNWISTQDVASACLISKKDVNVRAGKAFDLTGAEVISMERLKDIFEQD
                     LEMQIDLQCKNKEEAIRMLMQNNMPLDLIEWLVEYQILSSDDRLEPTTNILEQIIGHPP
                     NAAQLFENKQIN"
     operon          4487..9892
                     /function="maturation and modification of
                     plantathiazolicin"
                     /operon="ptnJDCBKL"
     gene            5204..6196
                     /gene="ptnD"
                     /operon="ptnJDCBKL"
     CDS             5204..6196
                     /codon_start=1
                     /db_xref="UniProtKB/TrEMBL:D3VML7"
                     /function="cyclodehydratase"
                     /gene="ptnD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RRE-containing: Plantazolicin_C_RRE"
                     /gene_kind="biosynthetic"
                     /operon="ptnJDCBKL"
                     /product="PtnD protein"
                     /protein_id="CBJ61637.1"
                     /sec_met_domain="Plantazolicin_C_RRE (E-value: 2.1e-40,
                     bitscore: 126.6, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSQQTHSIQTVVRFKLSPHITLGELEGSLLSFKAQSITKVDGEGI
                     VEFFEAISEYLVCENGVSIEELHQRFNLEKDQLRNVFDFLVTNGLCIQSTSNFDMTAFL
                     AYERAGGQVEVNQILHRLKTSIVEVVAHGENNLAINLTNSLQETGLNIDWKEKPDNGKP
                     SIRIAVAASHLDPLLEQVNDQSLEDHVPWLCVIPYDGQTAWVGPFFIPHKSACLHCYNL
                     RKSANFSDEVFRSELMKVKPVNAAKQPVYNQPVHLVQAGIANNLVTEWITLRDYAPSAV
                     PGGFITVNLDDRGVSVDNHRVYRVPRCQKCSPAADTGFPQVWYHGEVAK"
     gene            6169..7545
                     /gene="ptnC"
                     /operon="ptnJDCBKL"
     CDS             6169..7545
                     /codon_start=1
                     /db_xref="InterPro:IPR003776"
                     /db_xref="UniProtKB/TrEMBL:D3VML8"
                     /function="docking scaffold"
                     /gene="ptnC"
                     /gene_functions="biosynthetic (rule-based-clusters) LAP:
                     YcaO"
                     /gene_functions="biosynthetic (rule-based-clusters) LAP:
                     TIGR03604"
                     /gene_kind="biosynthetic"
                     /operon="ptnJDCBKL"
                     /product="PtnC protein"
                     /protein_id="CBJ61638.1"
                     /sec_met_domain="YcaO (E-value: 4e-69, bitscore: 223.4,
                     seeds: 127, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR03604 (E-value: 5.4e-113, bitscore:
                     367.2, seeds: 25, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVSRRGGQIIMVSKWTTLEDSWKVLDSLADPYVGIVRREYRKLRD
                     VDDVWGHSYGAHGTDSKILFGVPSNSYNGGGSDNAAAARLAAIGETVERYSAAYLPFDS
                     DVVCFGSQKELEKENKRFIGYKDWELYAPTQFKDPIFPHEVWNENTKLWWRKGTYAKTN
                     EEVWTPGQLIHIANHTEWPGDTNIGHATSNGLACGITFVEAAISGLFETIERDAFMLTW
                     YNKLSLPQIDVNSSPRLKRFYERYIKPTSLRLHLVDMTVFSGIPSVLAVVRNPHTNLAP
                     FAIGAASSYSIERACEKAAIEGMYTRTWVKTEQRLGNALVNADFNRDINSFEDHIKLYA
                     GTDIVKEADFLTSNSTLVDVNQFKSFDDSSPDVLWNSLNDHLYEKGFKVATFDLTSPDI
                     KDAGAYVVKSIIPGFKPIDVLYRGRMLGGERILRHSYDLGLVPDPFTIETLNPVPHPFP
                     "
     gene            7554..8381
                     /gene="ptnB"
                     /operon="ptnJDCBKL"
     CDS             7554..8381
                     /codon_start=1
                     /db_xref="GOA:D3VML9"
                     /db_xref="InterPro:IPR000415"
                     /db_xref="InterPro:IPR020051"
                     /db_xref="UniProtKB/TrEMBL:D3VML9"
                     /function="plantathiazolicin dehydrogenase"
                     /gene="ptnB"
                     /gene_functions="biosynthetic (rule-based-clusters) LAP:
                     PF00881"
                     /gene_kind="biosynthetic"
                     /operon="ptnJDCBKL"
                     /product="PtnB protein"
                     /protein_id="CBJ61639.1"
                     /sec_met_domain="PF00881 (E-value: 4.6e-19, bitscore: 58.8,
                     seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKGVNEMKEIERHATNLVDVVYGNEKLRFDDPSENWFEASKVYRS
                     SMAWDAPGVAGLMRSATLQKIATHAGKRFDDLPLITLPSPLRTSVSLDEVIQNRRSIEQ
                     FNGGSTTLAQLSTILQGSYGLIERPEGPRRPIPSGGALYPLDLYVVSNKVDSLEKGLYH
                     FDPYRKGLVHLGEYSEEDFGRIMLQEEAVKDFSFAVIISASFWRSRFKYGHRSYRFIFI
                     EAGHLMQNMILLATAQCIQSRPYGGFIDDELMDLIGGHNGVDDAPIYTLVAGT"
     gene            8378..9055
                     /gene="ptnK"
                     /operon="ptnJDCBKL"
     CDS             8378..9055
                     /codon_start=1
                     /db_xref="GOA:D3VMM0"
                     /db_xref="InterPro:IPR003675"
                     /db_xref="UniProtKB/TrEMBL:D3VMM0"
                     /function="zinc protease involved in plantathiazolicin
                     maturation"
                     /gene="ptnK"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /operon="ptnJDCBKL"
                     /product="PtnK protein"
                     /protein_id="CBJ61640.1"
                     /sec_met_domain="Abi (E-value: 2.7e-10, bitscore: 30.3,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMELRACIFIGILIHLMGPYVSKLTVMYQIKHRTKEYKLTRHITP
                     QRSYDIITVFGGIMCCSIFLLDDNFFLIHSNNAYGVLLFSALGFFFPYVIWKGKNKSVV
                     FVKRKVNKLDTLIFLPLCAVSEELIWRICIPFLFKLYFFDSVLLCTCISSIGFLLLHMP
                     LGGVKSAPYMTVFTVIAVIAAQFDFLSAAAFHIVHNLTIQYFHPVSKKKINTSSPTMSQ
                     AEW"
     gene            9080..9892
                     /gene="ptnL"
                     /operon="ptnJDCBKL"
     CDS             9080..9892
                     /codon_start=1
                     /db_xref="GOA:D3VMM1"
                     /db_xref="InterPro:IPR013216"
                     /db_xref="UniProtKB/TrEMBL:D3VMM1"
                     /function="methyltransferase involved in plantathiazolicin
                     modification"
                     /gene="ptnL"
                     /operon="ptnJDCBKL"
                     /product="PtnL protein"
                     /protein_id="CBJ61641.1"
                     /transl_table=11
                     /translation="MEIETIVRESEANRIQAQTWFSHPEKSKVSFRYDERETSSIRSIS
                     IETFLSFYSSKFNREPYSVLDIGCGQGQVIQYLNSRFQKIELTGIDSSAQAISSAKKLG
                     INASFICSNAENIMQYVSKKQDIIFIHLCFGLFKNPIAIVNTLIHLLSDQSCIYIVDLD
                     RNSLGEGLNTAQSREEEAYLKDQYRASLTMEEFKQLLHVVTKEQHGVSFHVGNSFIGGF
                     DETSSQFFSLMRNRNLQDALRTSVGEQLKQSQMPALLHGWIIKNKRYT"
ORIGIN
        1 atgccaaatc agaattttcg tgaatcagag atagctgaaa acttatctca tatcaaagtc
       61 ttgacgaact acaagactga gtactcaagg caaaccagtc catatttcat tatttggggc
      121 tgtatatgga ttattgcgta ctccgtttct ttatgtaagg taccttctgt agtccttgct
      181 tgtgtctggt taatactggc agtattaggg tggatactga ctttgaagac atatcttaag
      241 cagaaacaat ttgatcccat gcccactttc ttaaagaatc aattaaaata tgcatggatt
      301 ggattcatat taatggtatg catttttgtt ttcctgattt taatgggatt attgccgcga
      361 acaggagaat ttctatcgtt tttgattgtg ttaatggtgt ctattatgta catattctta
      421 ggcatcgtgt taacaaaaga gatttttgtt atgggatttt ggctgagcat actcggtatg
      481 cttacattct gcttacttcc taatgcaatg aatattgttt ttgcatttat tggcggaggc
      541 agcctattac ttacaggaat gattttgaaa agaaaagggc aaggaaatga gtaatgttaa
      601 caatatcaat gatttaatac atgccaaagt acggttggga attatgagtt tactgatgac
      661 ttatgatcaa tgtgatttta ctttcctgaa aaagtcatta gacatcacag atgggaattt
      721 aggttcgcat ctgaaaaagt tagaagatgc agcttatata gaactaacca agaagttcgt
      781 atccaaaaag ccgaagacta ttgttcagat tacgaaagaa gggaaagcag cctacaagga
      841 gtacattaat acaattgaat ccatatttaa aataaataac agtgggggaa tggtgtaaat
      901 gctgcaagtt gaacatataa gtaagcgcta tggcacgact ctggctttgg acaatgtggc
      961 tttaacaatt ccgaaaggag tctgctatgg acttgttgga ccgaacggat ccggcaaatc
     1021 taccttaatt aagatcatct cacgcatcat ccgggaatat gacggtaagg ttgaatttgt
     1081 cggtaatgaa aatggcagat tgggttatgt tccacaggat atcagccttg aagaaaaatt
     1141 aaccgcaaag actaacatgg aatttttcgg aaaggtacat ggactgaatg gcttaaaact
     1201 aaagcaaaca acaaaaaaac tgttagaaga tatcgggttg tcagaaagga caaacgacgt
     1261 tgtggagaac ttttccggag gaatgaagcg ccgtttaaat atcgggtgtg cattaatgca
     1321 cgatccagac ttaattattc tagatgagcc gacggtaggc gtggatccgc aatcacgccg
     1381 ctacattttt aacatcgtaa atcgactcaa aaaagccggt aaaaccatta tttatgtgag
     1441 ccattacatg gaagagattg aaactctttg tgactatgta gcttttatcg acagagggca
     1501 gattatcgat gaaggagaaa taaatgaact gcttaaacga cataaagaat catctattat
     1561 gtttaactcc tcattgtctc atgaattaat aaagagccat ggttttgagt tttcagtcgt
     1621 taattcaggg ctgataataa aaaccaaaaa acctttagaa accatggagc gcttaattag
     1681 cgtcagtaag gaaaacaata ttgtgcctca tcagttgttt ttatataagc caaaattaga
     1741 agatgtattc tttaaactta ccggcactga tttgcgtgat gaagaataaa gggggaattt
     1801 atcatgttac cgctcgctca aattgaaacg ctaaaacagt taaaggataa gtctctgttt
     1861 ttttggacga ttatattacc tattgtgtcc atcatattgc tgattgagct atttacgaca
     1921 gacagtacaa acaaaacttt agtagcttca caatctgtaa ccggtatgag cgtattcttt
     1981 tctacattca ttattatctc catcgttata agttttgtaa aggatcggga aaaaggaatt
     2041 gtatcaagac tggcaagtac ccctttaaag ccttataatt tcttcttagg aaaaagcatt
     2101 ccttttttag tcataacttt tatgcaaatt cttctccttt ctgttttagg aatatttctg
     2161 tatgatattg aaattaataa ttttccggcg tatctgtggg ttgttgtgtt gatatcttta
     2221 ctggttacat cctggggagt tgccatttca gtctttgcaa agactgaaaa taccggtttg
     2281 gtcatgacaa atgtgattgc attaggtacc gctgttgtat gtggtttatg gaccccttat
     2341 gatcaattac caagctatct tcaaactatt ggaatgctct ttccgcagta ctgggcgcat
     2401 caagggttaa ttcaatcggt ctcgacgata tcgggaggag aaagtctaat atgggtaacc
     2461 cttgttgtac tgggctatac aatactggga tttatagttt ctctaattgg ataccggcat
     2521 tacattaaaa tggcaagaaa ctagaccgct gaagaatggg tcattaaaac gcagctggga
     2581 ctaaaaggag agcgggaaat gataagtgaa aaactaaaac tgtatgtcga ggctctattt
     2641 aaaaagtacg aaagaaattc gttactgagc agaaaaaaaa cggagctttt aactaagctt
     2701 catgatagaa gtattagcct ggaagcagag ggtatgacaa agctggatat tgagaagttg
     2761 cttatacgcg aaatagaatc caaaagcttg gcggtcgata ccagtgacct gaatatagat
     2821 aaatccaatg tattaaaact caaaaaaaag acgtttataa ataagaattt gcaaaaaacg
     2881 gaggattttg agccggtaaa tgccgagtac tatctcagtg actttaaaag tgcaactctt
     2941 cagaatatcg atgtcgaaca ctctgtcttc aaaaactgct acttcaagaa tttctcatgt
     3001 aaagactccg ccatcataga atcgacattt aaaaaaagtg atctcagcca gtcaaattat
     3061 gacacatgta aacttgagta tatgctttat accggttgtc acctgccaaa agtcaatttt
     3121 acagatacct ccatcctgca tacgttcttc aagaactgct atctaaagaa agtatccttt
     3181 acgaactgca ttctaattaa tattcggttt gaatcttgtg atattgcaaa cgttaaaatt
     3241 acaggaggca aaatggataa aacaacatac cgtatactgc aagaagaggg gacttcatta
     3301 cattcagtag aattaattta aatttaatat aaagaagcat agaccaataa ggatcatctt
     3361 attggtctat ttgtcttaga ggttaaaacg tagatgaact agagatgatt gtggtacagg
     3421 tacagcgtgc agccattggc tcgaataaat gttgaccttc tccgtgtaca cttgcaataa
     3481 gagcagttgg cactttaatt tgagtcataa ttgttacctc ctccataaaa tttatcgaca
     3541 aagaattcac cgatatgtac catattgtga tttatatgga caatattgtc aacccaaata
     3601 tgtaattttt tgtgatatag ataatgaatt tataatgagc aatttggttt aagaagctga
     3661 atcactctaa atttagttaa aaatgaaatt aataattctg aattttatat ttgatcagag
     3721 ggaaagtatg tgttttaata gggttatggg aataaatact attttttaag cggaataacc
     3781 caacttgtta ggaaaaagga aacgttgttt gaagttaaag ctaagctaat tcaggcgaat
     3841 cgcataggct gatcggggct ttgggtggat tcttaacagg aaatgtcaaa cttcaaggaa
     3901 gcttgagctt tgtctgttgg acaaaagggg tgactgcccg actgcttaga ataaagctta
     3961 atttactaag ggtttcttgt accccattgg tattgtaatg attctggtgt cacctacgaa
     4021 agatttactc ttctttgtat tacctcagca ttcttcagaa gcttcttcag atagtattac
     4081 aaaatcaata attgatttaa tgatatttgt gaattcgtaa ctccctgtat tattttgtcg
     4141 cacagagaga tgaaccaggc cgcccctggc gatctaaagt aggaggaagt aatacgttat
     4201 taacttattt gttttctcaa gacaccatcc tttgtttact tttaagaaag gtatttaact
     4261 tctgatccta actaacttaa tcttgtatgc tgttttctat ttgctgctgc actgtcctaa
     4321 cttttatggt tcaaccatct tttgttaaga catttattta cggtgattat caaatttata
     4381 atttctatat taagtattaa ttggttatat taactttttt gcttatcttt ctgatgacaa
     4441 atcatgagat tgatcgagtg agattattga ctaggaggta ttggacgtga aaattcacta
     4501 catgggagtt agggaacccc tgaaacaaat attggaggat aaggcggctg agctccatga
     4561 ttatccgctg cagattgtag atttaggcgg atcaataaag tacgaaagtg attctttcat
     4621 caactctttt gacttggatg ctgaaacaat catactttca aaccggagat ataatttgaa
     4681 tgatgaattg ggttacgtgg aaacagagtt actacaaaaa tataaaaaaa gtattttata
     4741 cagaacatcc cctttggata tcggaatcga gttgtacaga ggatgggcag cagccacagg
     4801 aatactgctc actgcttttt ctccggtagg cgtaaattgg atttccactc aagatgttgc
     4861 atctgcatgc ttaatctcaa aaaaagatgt aaatgttcgc gcagggaaag cctttgacct
     4921 aaccggagcg gaagttatta gtatggaaag actaaaagat atatttgaac aagatttgga
     4981 aatgcagata gatttacaat gtaaaaataa agaggaagcg attaggatgc tgatgcaaaa
     5041 taacatgcca ctggatctaa tagaatggtt ggtagaatat cagatactgt cttccgatga
     5101 ccgactagaa ccaactacta atattctgga acagataatt gggcatccgc caaatgcagc
     5161 tcaattattt gaaaataaac aaattaatta aggaggaata cagatgtctc aacaaactca
     5221 ctctattcaa acggttgtaa ggttcaaatt atctcctcat attactttgg gagaattgga
     5281 aggaagcctg ttaagcttta aagcacaatc cattacaaaa gttgatggag aaggaatcgt
     5341 tgaattcttt gaggcaattt cagaatatct tgtatgtgag aatggagtct caattgaaga
     5401 actgcaccaa aggttcaacc tagagaaaga tcagttaaga aacgtcttcg atttcctggt
     5461 gacgaacgga ttgtgtatac aatctacttc taattttgac atgactgctt ttttggcata
     5521 tgagagggca ggggggcaag tggaagtcaa ccagatctta catagattaa agacaagcat
     5581 agtggaagtt gtagcacatg gggaaaataa cctagcaata aacctaacta atagcttgca
     5641 ggaaacagga ttaaacatag attggaaaga aaaaccggat aatgggaagc cttcgatacg
     5701 tattgctgta gcagcttcac atttggaccc gctgttagaa caagtaaatg atcagtcatt
     5761 agaagatcat gtcccttggt tgtgtgttat tccatacgat ggtcagacag cttgggttgg
     5821 tccatttttc atcccgcaca agtctgcatg tctccattgt tataacctac gtaaatcagc
     5881 aaacttttca gatgaggtat ttcgttcaga attgatgaag gtcaagccag tcaacgctgc
     5941 gaaacaaccg gtatataatc agccagttca tttggtacag gcaggaatag cgaataatct
     6001 tgtcacagag tggattacac taagagacta tgcaccaagc gccgttccag gtggttttat
     6061 aactgtcaat ttggatgaca ggggagtttc agttgataac catcgtgtat atagagtacc
     6121 gagatgccaa aagtgttctc ctgccgctga cacagggttt cctcaagtat ggtatcacgg
     6181 cgaggtggcc aaataattat ggtgagtaaa tggacgacat tagaagattc atggaaagtc
     6241 cttgatagtc ttgctgatcc atatgtaggt atagtgagaa gagagtatcg gaaattacgc
     6301 gatgtagatg acgtttgggg acactcgtac ggtgctcatg ggacagacag taaaatttta
     6361 ttcggagtcc cctctaattc ttataatgga ggcggatcgg ataatgcggc tgcggcaaga
     6421 ctagctgcta ttggggaaac ggtggaaaga tatagtgcag cttatcttcc atttgattct
     6481 gatgttgtat gttttggatc acaaaaggaa ttagagaagg agaataagcg tttcattgga
     6541 tataaagatt gggaattgta cgctccaact caattcaaag atcctatatt tcctcatgaa
     6601 gtttggaatg aaaatactaa gctgtggtgg agaaaaggga catatgctaa aacaaatgag
     6661 gaggtatgga ccccagggca actaatccat atcgccaatc atacggaatg gcctggggac
     6721 actaatatag gtcatgctac aagtaatggt cttgcttgcg gaattacctt tgtagaagca
     6781 gcaatttcag gtttatttga aacgattgaa cgggatgctt ttatgcttac ctggtataac
     6841 aagctgagct tgccccagat tgatgtgaat tcttctccga gattaaaacg cttttatgaa
     6901 agatatatta aaccaacatc attgcggcta cacttagttg atatgactgt attttcaggt
     6961 attccttctg tgctagcagt tgtcagaaat cctcatacga atttagcccc ttttgcaatt
     7021 ggagccgcct cctcctattc aatagaaagg gcttgtgaaa aggcagcaat tgaaggtatg
     7081 tatacaagaa cttgggtcaa gacggaacaa cgtttaggta acgcattagt gaatgcggat
     7141 tttaataggg atatcaatag ttttgaggac catatcaagc tttacgcagg aaccgatatc
     7201 gtcaaggaag ctgatttcct tacaagtaac agcactttag ttgatgttaa ccaatttaag
     7261 tcattcgatg atagttctcc tgatgtactt tggaatagtc taaatgatca cctatatgaa
     7321 aaaggtttca aagtggctac atttgattta acaagtcctg atataaaaga tgccggagct
     7381 tatgtagtaa agtccatcat tccagggttt aaacctatag atgtccttta caggggaaga
     7441 atgttgggtg gagaacgtat attacgacat tcttacgatt tagggttagt accagacccg
     7501 tttacaatcg aaacacttaa tccagtacct catccattcc cataaatact aaggtgaaag
     7561 gggttaacga aatgaaagag attgaaaggc atgccactaa tttggttgat gtagtctatg
     7621 gcaatgaaaa actaagattt gatgatcctt ctgagaactg gtttgaggct tcgaaagttt
     7681 atcgaagctc tatggcgtgg gacgcgccag gagtagcagg cctaatgaga tctgcaacat
     7741 tacagaagat cgctactcat gcaggtaaac gatttgatga cttgccattg attacattac
     7801 cttccccatt gaggacaagt gtatcattag atgaagttat tcaaaatagg agaagtatcg
     7861 aacagtttaa cggtggtagt acaacattag cacagctttc taccattctg cagggtagtt
     7921 atggattaat agagcgccct gaaggtccta gacgccctat tccttctggc ggagctcttt
     7981 atccgttgga tctgtacgtt gttagcaaca aggtggattc tttggaaaaa ggtctatatc
     8041 actttgatcc ttatcgtaag ggcctagttc atctaggtga gtactctgaa gaggatttcg
     8101 gaagaataat gttacaagaa gaagctgtaa aggacttttc tttcgctgta atcatatcgg
     8161 ctagcttttg gaggtcaagg tttaaatatg gccatcgttc atatcgattt atatttattg
     8221 aagcgggtca tctgatgcag aatatgattt tattggccac agcccaatgt atccagagcc
     8281 gtccatacgg agggtttata gatgatgagc tcatggatct tattggtggc cataatggag
     8341 tagacgatgc ccctatatat actttagttg caggaacatg atggagttaa gggcatgcat
     8401 ctttataggg atcctgattc atttgatggg tccctatgtt tcaaagctaa cagttatgta
     8461 tcaaatcaag catagaacta aggaatataa actgactcgt catataacac cgcagcgcag
     8521 ttatgatatt atcaccgttt ttggtggaat tatgtgttgc agcatcttct tattagatga
     8581 taactttttt ttaatacata gcaataatgc gtacggtgtt ttattattta gtgccctagg
     8641 tttctttttt ccttatgtta tctggaaggg gaaaaacaaa tccgttgtat ttgttaaaag
     8701 aaaagtaaat aaacttgata cattgatttt cctgccttta tgcgcagttt cagaggaatt
     8761 aatatggaga atttgtattc cttttctatt taaactatac ttctttgatt ctgtcttatt
     8821 gtgtacgtgt attagctcga taggattctt gctgctccat atgccccttg gtggtgtaaa
     8881 gtcagccccc tatatgactg tatttactgt aatagctgta atagctgcac aatttgattt
     8941 tttatcagca gctgcattcc atattgtgca taatttaact atccagtatt ttcatcctgt
     9001 cagcaaaaag aaaataaata catcttcgcc gacaatgtct caagcagagt ggtaaactct
     9061 ttggaggtgt cacagttata tggaaattga aacaattgtc agagagtcag aagccaacag
     9121 aattcaagca caaacatggt tttctcatcc tgagaaaagt aaagtgagct ttcggtatga
     9181 tgagagggaa acctcatcca ttagaagtat ttcaattgaa acatttctgt cgttttactc
     9241 cagtaaattt aatagagagc cgtattctgt attagatatt ggttgcggac aggggcaagt
     9301 aattcaatat ttgaacagtc ggtttcaaaa gatcgaactg acaggtattg attcctctgc
     9361 ccaagcaatt tcttcagcaa aaaaactcgg tattaatgca tcttttatat gttcaaatgc
     9421 agaaaatatt atgcaatatg tgtctaagaa gcaagacatt atattcatac atctatgttt
     9481 tggactcttt aagaatccta ttgctattgt aaatacactc atccatctat tatcagatca
     9541 atcatgtatc tatattgtag acttggacag gaacagtttg ggagagggcc ttaacacagc
     9601 gcaatctcgt gaggaagaag cctatttaaa agatcaatat cgtgcttctt taacaatgga
     9661 agaatttaaa cagctcctgc atgttgtgac caaagaacaa catggggtgt catttcatgt
     9721 aggtaattcc tttattggcg ggtttgatga aaccagttca caattttttt cgctaatgag
     9781 aaacagaaat ttgcaagacg cgttaaggac cagtgttgga gaacagttaa agcagtcaca
     9841 aatgccagct ctgctgcatg gttggattat aaaaaacaaa aggtatacgt ga
//
