LOCUS       BGC0000570              3527 bp    DNA     linear   BCT 14-MAY-2014
DEFINITION  Asticcacaulis excentricus CB 48 chromosome 1, complete sequence.
ACCESSION   BGC0000570
VERSION     BGC0000570
KEYWORDS    .
SOURCE      Asticcacaulis excentricus CB 48
  ORGANISM  Asticcacaulis excentricus CB 48
            Bacteria; Proteobacteria; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Asticcacaulis.
COMMENT     URL -- http://www.jgi.doe.gov
            JGI Project ID: 4085008
            Source DNA and organism available from Yves V. Brun
            (ybrun@indiana.edu)
            Contacts: Yves V. Brun (ybrun@indiana.edu)
            Tanja Woyke (microbe@cuba.jgi-psf.org)
            Annotation done by JGI-ORNL and JGI-PGF
            Finishing done by JGI-LANL
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. it is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376).
            ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-07 19:53:56
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3527
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3527
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[1179:3527]"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1180..3527
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
     cand_cluster    1..3527
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /kind="single"
                     /product="lassopeptide"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..3527
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000570"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..3527
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lassopeptide"
                     /region_number="1"
                     /rules="(PF13471 and Asn_synthase or micJ25 or mcjC)"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            239..856
                     /locus_tag="Astex_2227"
     CDS             239..856
                     /codon_start=1
                     /db_xref="GO:0003700"
                     /db_xref="InterPro:IPR000524"
                     /inference="protein motif:PFAM:PF00392"
                     /locus_tag="Astex_2227"
                     /note="PFAM: regulatory protein GntR HTH; KEGG:
                     sjp:SJA_C1-30010 GntR-family transcriptional regulator"
                     /product="regulatory protein GntR HTH"
                     /protein_id="ADU13883.1"
                     /transl_table=11
                     /translation="MAHPSAFLRVHTEIGRRLTQGVYPPGHRLEAEPLADEMGTSPSPV
                     RQSLYWRCGEGLLTHHVLGGFTVPVLTEFGLRHLFSWQSELWRMGYACFKPAPAGAPLL
                     LEIERLSSQPVSCLDHILGTIVFSHPNREVHRAWRLTTQRLAPSQFKRHEGPDPLMAWC
                     TAFSTALSSLNIGDLEACASEYFQTSLDIVPQIAQACQPTNG"
     gene            970..1125
                     /locus_tag="Astex_2228"
     CDS             970..1125
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (lassopeptides)
                     predicted lassopeptide"
                     /gene_kind="biosynthetic-additional"
                     /inference="similar to AA sequence:KEGG:THAPSDRAFT_270227"
                     /locus_tag="Astex_2228"
                     /note="KEGG: tps:THAPSDRAFT_270227 hypothetical protein"
                     /product="hypothetical protein"
                     /protein_id="ADU13884.1"
                     /transl_table=11
                     /translation="MHTPIISETVQPKTAGLIVLGKASAETRGLSQGVEPDIGQTYFEE
                     SRINQD"
     CDS_motif       970..1053
                     /prepeptide="leader"
                     /note="peptide class: lassopeptide"
                     /note="predicted sequence: MHTPIISETVQPKTAGLIVLGKASAETR"
                     /locus_tag="Astex_2228_lassopeptide"
                     /aSTool="lassopeptides"
                     /tool="antismash"
     CDS_motif       1054..1113
                     /prepeptide="core"
                     /locus_tag="Astex_2228_lassopeptide"
                     /peptide="lassopeptide"
                     /predicted_class="Class II"
                     /score="14.70"
                     /molecular_weight="2564.7"
                     /monoisotopic_mass="2563.2"
                     /core_sequence="GLSQGVEPDIGQTYFEESR"
                     /aSTool="lassopeptides"
                     /tool="antismash"
                     /leader_sequence="MHTPIISETVQPKTAGLIVLGKASAETR"
                     /leader_location="[969:1053](+)"
                     /tail_sequence="INQD"
                     /tail_location="[1113:1125](+)"
     CDS_motif       1114..1125
                     /locus_tag="Astex_2228_lassopeptide"
                     /note="tail peptide"
                     /note="lassopeptide"
                     /prepeptide="tail"
                     /aSTool="lassopeptides"
                     /tool="antismash"
     gene            1180..1809
                     /locus_tag="Astex_2229"
     CDS             1180..1809
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: PF13471"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lasso_Fused_RRE"
                     /gene_kind="biosynthetic"
                     /inference="similar to AA sequence:KEGG:Caul_5192"
                     /locus_tag="Astex_2229"
                     /note="KEGG: cak:Caul_5192 hypothetical protein"
                     /product="hypothetical protein"
                     /protein_id="ADU13885.1"
                     /sec_met_domain="PF13471 (E-value: 6.8e-27, bitscore: 82.0,
                     seeds: 84, tool: rule-based-clusters)"
                     /sec_met_domain="Lasso_Fused_RRE (E-value: 1.7e-12,
                     bitscore: 35.6, seeds: 20, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYELNDGVGLALVDQHPIFLDLKTDRYLSLSPDGAAVLLGAAPAT
                     KESPLFLGLESIGLVKNGPSGLKPCQIAVATGSAPPRKVQFESLSLLLLRLIRARLDQR
                     ALLKRVTDLKKAGTIAQTKNRDCALSLLGSVETEAKACRTLLSSTDKCLPDAFAIATHL
                     RRRGVDAKLVFGVRLPFAAHAWVQVDDIVVGDRPDRILAFTPILVV"
     gene            1809..3527
                     /locus_tag="Astex_2230"
     CDS             1809..3527
                     /codon_start=1
                     /db_xref="GO:0004066"
                     /db_xref="InterPro:IPR001962"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Asn_synthase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177:asparagine synthase (glutamine-hydrolyzing)
                     (Score: 117.8; E-value: 8.7e-36)"
                     /gene_kind="biosynthetic"
                     /inference="protein motif:PFAM:PF00733"
                     /locus_tag="Astex_2230"
                     /note="PFAM: asparagine synthase; KEGG: sal:Sala_2602
                     asparagine synthase"
                     /product="asparagine synthase"
                     /protein_id="ADU13886.1"
                     /sec_met_domain="Asn_synthase (E-value: 1.5e-13, bitscore:
                     39.1, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRYVASFFVRGHVSTPALRHPEPKGFAYAKVSGGLSVWSDAPIRH
                     RAPLITVGAVFDRASFKGLDCDLSGLRQDGLNTLKAETFGPYLALEVADNGTLRVYRDP
                     SGGAPCYYLQTEDGFWLASDADLLFTHSGVHPSVSLPGLIEHLRRPEFQNEGTCLNVKQ
                     VRPGEQVDLSLSGEVRACLFPPASSLRPPELHRAYDDIKAELRALILRSIKAYASDFPH
                     VVVSFSGGLDSSVVAAGLAQTSTKVLLHTFKGPDAKGDETAFAAECAAYLGLSLEIDTL
                     SIDDVDLSATISPHLPRPSTSFFLPSLLRGFSTSSQTRTGGAIFSGNGGDSVFCFMHSA
                     TPLADLMCRPSGLTPFMQTWADVQKLTRASATEVLRRALKTAMARGYIWPESNLLLSRD
                     TSSSRLTPDSVLSSLEGILPGRLRHLALIRRAHNTFEPFAPWRTPPVVHPLMAKPIQAF
                     CLSLPSWMWVSGGKDRSLVRDAFEGLLPDSVRLRKSKGSPAGFLHALYRAKGRQMIERI
                     RHGYLRREGIIDISTGPDALFSEGFRNPRVMHRFFELAATEVWIDHWRNWRRPRT"
ORIGIN
        1 tctggaaaaa tcctaaaaat gagcgtttga ataatgcgct gcaaaaacgg gaccattatg
       61 cgcctgttag gcgaacttca gcaaggggcg cgacgatagg ccacctattc tcgtctactt
      121 ttgacatatt tccgggcctg taagttttct attggacagg tctatatttt tcatattcta
      181 ttttttggat taacattcat tttacaatta tagatggaaa tgcctcttag aggttcgtat
      241 ggcacacccc tcggctttct tacgggttca cacagaaatc ggaagacgat tgacgcaggg
      301 cgtctatcca cccggacatc gcctggaggc cgaaccgctg gctgacgaga tgggaacaag
      361 ccccagtccc gttcggcaat ccctttactg gcgctgtggc gaaggtcttc tcacgcatca
      421 cgtgctaggc gggttcaccg tgccggttct aaccgagttt ggacttcgtc atcttttttc
      481 gtggcaatca gagctttggc gcatgggcta tgcctgtttc aaaccggcac cagcgggggc
      541 gccacttttg ctcgagattg agcgtctgag ctcgcagccc gtctcatgcc tcgaccatat
      601 tttgggaacc attgtcttta gtcacccgaa ccgggaagtg caccgcgcat ggcggctgac
      661 gacgcaaaga ttagccccat cgcaattcaa acgccacgaa ggccccgatc cgctcatggc
      721 ctggtgcacg gcgttttcta ccgcgctctc gtccctcaat atcggtgatt tagaggcgtg
      781 cgcttctgag tattttcaaa cgtctttgga tatcgttccg caaatcgcac aggcctgtca
      841 gcccacgaat ggataaggcc agcacagatt tctcgtctat catttagtta tcaattccaa
      901 acagccgcgc acaggcgaac tcttggtctt gccatgaatg gtcatggcta aatgaaagga
      961 gagttcgaaa tgcatacccc cattatttcg gaaacggtcc aaccgaagac cgctggcctc
     1021 atcgttctgg gcaaggcttc agctgagacc cgcggtctca gccagggtgt cgagcccgat
     1081 atcggccaga cctacttcga agagtctcgc atcaaccagg actgacgacg ggaaggcgcg
     1141 cacactgcgc gccttcttgc cttttcaggg gcttttgaga tgtacgaatt aaacgacggg
     1201 gtgggactgg ccctggtaga tcagcatccc atcttccttg atctcaaaac tgaccgctac
     1261 cttagcctct cgcctgacgg cgcagctgtg cttttgggcg ccgcacccgc gaccaaggag
     1321 agccccctct tcttaggact tgaatccatc ggcttggtta aaaacggccc ctcagggctg
     1381 aagccttgcc agatcgccgt tgccacagga agtgccccgc ccaggaaagt gcagtttgag
     1441 tcactgagcc tgctattgtt gcgcctgatc cgcgcaaggc tcgaccagcg cgccttactc
     1501 aaacgagtga cggatttgaa gaaggccggc acgatcgcgc agaccaaaaa ccgcgattgt
     1561 gccctgagcc tgcttggatc ggttgaaacg gaggcgaagg catgccgtac actcctgtca
     1621 tcaacagaca agtgcttacc cgatgcgttt gcaatagcca cgcatctgag acgccgaggt
     1681 gttgacgcaa agctcgtctt tggtgtccgc ctgccttttg cggcccatgc ctgggttcag
     1741 gtcgacgata ttgtcgtggg tgaccggcca gaccggattt tggcgttcac ccccatcctg
     1801 gtcgtctgat gcggtacgta gcgtctttct ttgtgcgagg ccacgtaagc acgccggcat
     1861 tacgacatcc agagccaaag ggcttcgcat acgcaaaggt atctggcgga ctaagtgtgt
     1921 ggtcggacgc ccccatccga catcgggctc cgctcatcac ggttggcgcc gtgtttgatc
     1981 gcgcttcgtt taaaggactc gattgcgacc tcagcggatt gagacaagat ggactgaata
     2041 cgcttaaggc ggagacgttc ggtccctatc tggcgcttga ggtggccgac aatggcacac
     2101 tgcgggttta ccgggaccct tcgggcggcg cgccctgcta ctatcttcaa acggaagacg
     2161 gtttctggtt ggcctcagac gccgatcttc tgttcaccca tagtggtgtg catcccagcg
     2221 tctcattgcc agggctaatc gagcatttac gaagaccgga gtttcagaat gagggcacct
     2281 gtctcaacgt gaaacaggtg cgcccgggcg agcaggtgga tctgtcatta tcgggggagg
     2341 taagagcctg cctattccca ccagcctctt ctctccggcc tccagagctg caccgcgcct
     2401 atgacgacat aaaggccgaa cttagagctc tcatcctcag atcaatcaaa gcctatgcca
     2461 gtgacttccc gcacgttgtg gtgagtttct caggcggcct cgattcctct gtggttgccg
     2521 cgggattggc gcagacctcg acaaaggttt tgctacacac cttcaaaggc ccggatgcca
     2581 agggtgatga aacggcgttt gccgccgaat gtgccgccta tcttggactt agccttgaaa
     2641 tagatacgct cagcattgat gacgtcgatc tttcggcgac gatctctccc catctgccac
     2701 gtccctcaac ctcgtttttc ctcccgtccc tcctcagagg cttttcgacg tcttcccaga
     2761 cccgaaccgg aggggctatc tttagtggca atggcggaga cagtgtcttt tgtttcatgc
     2821 attctgccac cccactcgca gacctgatgt gcaggccctc aggtttaacg ccattcatgc
     2881 aaacctgggc agacgtgcaa aagctgacgc gtgcgagcgc aacagaggtg ctcaggcggg
     2941 ctttgaagac cgcgatggca cggggctaca tctggccaga aagcaatctt ttgctcagcc
     3001 gtgacacctc ttcctcgcgc cttacccctg actccgttct ttcttccctt gaagggatcc
     3061 tacctgggcg gttacggcat ctggcgttaa tcagacgagc tcacaacacg tttgaaccct
     3121 ttgcgccgtg gcgcacaccc ccggttgtcc acccgctgat ggcgaaacct atacaggcct
     3181 tttgcctgtc gctcccaagc tggatgtggg tgtcaggcgg taaggatcgg tcactcgttc
     3241 gcgatgcttt tgagggtctg ttacccgaca gtgtgcgcct acggaaatcg aagggaagcc
     3301 ccgcaggctt tctacatgct ctatacagag ccaaagggcg ccagatgatt gagcggatcc
     3361 gtcatggata tctcaggcgc gaaggaataa tcgacatctc aacgggtccg gacgctctct
     3421 tttcggaagg gtttcgcaat cccagggtca tgcaccgctt ttttgagctc gcggccacgg
     3481 aggtgtggat tgaccactgg cgaaactggc gccgtccgcg gacctaa
//
