LOCUS       BGC0000571              4574 bp    DNA     linear   BCT 27-FEB-2015
DEFINITION  Burkholderia rhizoxinica HKI 454, complete genome.
ACCESSION   BGC0000571
VERSION     BGC0000571
KEYWORDS    .
SOURCE      Mycetohabitans rhizoxinica HKI 454
  ORGANISM  Mycetohabitans rhizoxinica HKI 454
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Mycetohabitans.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-07 19:54:07
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4574
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4574
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[1817:4387]"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1818..4387
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
     cand_cluster    1..4574
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /kind="single"
                     /product="lassopeptide"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..4574
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000571"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..4574
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lassopeptide"
                     /region_number="1"
                     /rules="(PF13471 and Asn_synthase or micJ25 or mcjC)"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(1..1800)
                     /locus_tag="RBRH_03778"
     CDS             complement(1..1800)
                     /codon_start=1
                     /db_xref="EnsemblGenomes-Gn:RBRH_03778"
                     /db_xref="EnsemblGenomes-Tr:CBW74823"
                     /db_xref="GOA:E5AQE1"
                     /db_xref="InterPro:IPR001140"
                     /db_xref="InterPro:IPR003439"
                     /db_xref="InterPro:IPR003593"
                     /db_xref="InterPro:IPR011527"
                     /db_xref="InterPro:IPR017871"
                     /db_xref="InterPro:IPR027417"
                     /db_xref="UniProtKB/TrEMBL:E5AQE1"
                     /function="Multidrug resistance ABC transporter ATP-binding
                     and permease protein"
                     /gene_functions="transport (smcogs) SMCOG1288:ABC
                     transporter related protein (Score: 269.6; E-value:
                     9.3e-82)"
                     /gene_kind="transport"
                     /locus_tag="RBRH_03778"
                     /note="Multidrug resistance ABC transporter ATP-binding and
                     permease protein; COG: ABC-type transport system involved
                     in Fe-S cluster assembly, permease and ATPase components;
                     Pfam: ABC transporter transmembrane region::PF00664<br>ABC
                     transporter::PF00005"
                     /product="Multidrug resistance ABC transporter ATP-binding
                     and permease protein"
                     /protein_id="CBW74823.1"
                     /transl_table=11
                     /translation="MRHGLFKTIFFLLGCLWTREWRTRLQLIAAIGCTGCSVGLTIVSP
                     MLLKWLIDGLSGASLSATSVYLLCVAYGGAWFLSQATHRCEEYLFVVVNERIKRTITLN
                     YVSRVLGQPAAALSAHQTGASVSELTRVQDSAMRAIIGLFWSLAPICVEIVVVGIVVCS
                     LFGWVYAAVLLGILTLYLYFSVATAKRCEVLQRKKNRASDRASTRLVDTLTHADTVKAF
                     SNEASELARLNRVFKMRAWASIHIARKMEIVGAAQLLIVGIGLTALTALAGRDILAHRL
                     TIGDFVLLNSYLLRFTLPLSTFGYMLRGIQIGLFNLSELLHTIRVRWRFDPLGQPLFEQ
                     APSIEFRNASFCHPCGRRVLRHANFSIPAGAFCALVGSSGAGKSTLIKLLLRLYKLDEG
                     QILIEGQPIESFSESQLRQSIGYVTQEAHLLDRSIRENICFAYPQASDSELESVIQTCA
                     LTPVIAAASQGLDTSIGERGNKLSGGERQRVGLARALLRNSPLLLLDEPTAALDAHTEA
                     DIFKHLYERYRHTTRLVIAHRLAAIQQADLILVMQEGAIVETGTHRSFWRNRAFMPICG
                     INKLIHRQHDLTASIRQKQSMRR"
     gene            complement(1818..3560)
                     /locus_tag="RBRH_03777"
     CDS             complement(1818..3560)
                     /codon_start=1
                     /db_xref="EnsemblGenomes-Gn:RBRH_03777"
                     /db_xref="EnsemblGenomes-Tr:CBW74824"
                     /db_xref="GOA:E5AQE2"
                     /db_xref="InterPro:IPR001962"
                     /db_xref="InterPro:IPR014729"
                     /db_xref="InterPro:IPR029055"
                     /db_xref="UniProtKB/TrEMBL:E5AQE2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Asn_synthase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177:asparagine synthase (glutamine-hydrolyzing)
                     (Score: 84.2; E-value: 1.3e-25)"
                     /gene_kind="biosynthetic"
                     /locus_tag="RBRH_03777"
                     /note="unnamed protein product"
                     /protein_id="CBW74824.1"
                     /sec_met_domain="Asn_synthase (E-value: 7.1e-18, bitscore:
                     53.4, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTLLGIAYPQAQDDWAQKQDWTLVRDPSHRTAGALWHQSEPSTRV
                     LCGRLGTSAYYFYGSAFSKQDHREIQEGELQAMVAEGPARLMAQCWGRYLFISFDLAAK
                     KLICCSDPSNQWPLYWAWSNRDGLLFSDTITCLHQTLVERGERPAWNHAFFTTWLRTGT
                     MQSGELPFDRIRELPVGCALSYTEGRQPQIVTVWDPLKHAIKDDTQTPFDILKHYLNRF
                     VSFSDRPVLELSGGLESSSVLLAMRAVASADQPLSCAHYYHADVASSNELDHARQVAQH
                     TQAHLQEVNGKALTFAPVQSEPPRMAKPSLRYSVLARGQYCASQFERQEHTPLISGHGG
                     DALFLAPPPCSALADAALTGQWRRLRRMAMDLALMYRAPLMRVAKRAVQSVFASDPSDK
                     VNDTFKLLSEDTQHILFDRRTCLHPFFQHTQTRLPGKLDQLFMAFLSLDDVRIPAYPFT
                     QPKHYPFLCQPMVEFALSTPSYDHFEGAHNRIVLRKSVAAATGYPNLWRRNKGETSGID
                     LLGLRDHREHVMAVCLEGWLAKAGYIDVARTHDAIQQSSKGNSKHFMDLLHLYAAELFI
                     QSWQ"
     gene            complement(3557..4387)
                     /locus_tag="RBRH_03776"
     CDS             complement(3557..4387)
                     /codon_start=1
                     /db_xref="EnsemblGenomes-Gn:RBRH_03776"
                     /db_xref="EnsemblGenomes-Tr:CBW74825"
                     /db_xref="UniProtKB/TrEMBL:E5AQE3"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: PF13471"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lasso_Fused_RRE"
                     /gene_kind="biosynthetic"
                     /locus_tag="RBRH_03776"
                     /note="unnamed protein product"
                     /protein_id="CBW74825.1"
                     /sec_met_domain="PF13471 (E-value: 1e-21, bitscore: 65.4,
                     seeds: 84, tool: rule-based-clusters)"
                     /sec_met_domain="Lasso_Fused_RRE (E-value: 1.7e-11,
                     bitscore: 32.3, seeds: 20, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQHGAWRQSQLCEAITGCFPSATRYNYHTHMPHHLMPHVFGACFA
                     QQIILLDVVRDRYAVLTERQSAALAQQLGLSDARFARVEAVQSASILEELKRAQWISVT
                     SFAEADAKLNMLSPSPTTGGMSANGWKLPEDAFARRPAWTRIVKALFLLRSVHRCAARQ
                     GLAGLLQLCHQAHREASLRIGLPDDYAPFVDALNWACLFYPRSTKCLEWSVALTVLCAR
                     AGLALKLVIGVQSFPFYAHAWSEAGGAVIGDSPLRRKELAVILECPHPLLDLSV"
     gene            complement(4422..4574)
                     /locus_tag="RBRH_03775"
     CDS             complement(4422..4574)
                     /codon_start=1
                     /db_xref="EnsemblGenomes-Gn:RBRH_03775"
                     /db_xref="EnsemblGenomes-Tr:CBW74826"
                     /db_xref="UniProtKB/TrEMBL:E5AQE4"
                     /gene_functions="biosynthetic-additional (lassopeptides)
                     predicted lassopeptide"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="RBRH_03775"
                     /note="unnamed protein product"
                     /protein_id="CBW74826.1"
                     /transl_table=11
                     /translation="MNKQQQESGLLLAEESLMELCASSETLGGAGQYKEVEAGRWSDRI
                     DSDDE"
     CDS_motif       complement(4494..4574)
                     /prepeptide="leader"
                     /note="peptide class: lassopeptide"
                     /note="predicted sequence: MNKQQQESGLLLAEESLMELCASSETL"
                     /locus_tag="RBRH_03775_lassopeptide"
                     /aSTool="lassopeptides"
                     /tool="antismash"
     CDS_motif       complement(4440..4493)
                     /prepeptide="core"
                     /locus_tag="RBRH_03775_lassopeptide"
                     /peptide="lassopeptide"
                     /predicted_class="Class II"
                     /score="2.70"
                     /molecular_weight="2522.5"
                     /monoisotopic_mass="2521.1"
                     /core_sequence="GGAGQYKEVEAGRWSDR"
                     /aSTool="lassopeptides"
                     /tool="antismash"
                     /leader_sequence="MNKQQQESGLLLAEESLMELCASSETL"
                     /leader_location="[4493:4574](-)"
                     /tail_sequence="IDSDDE"
                     /tail_location="[4421:4439](-)"
     CDS_motif       complement(4422..4439)
                     /locus_tag="RBRH_03775_lassopeptide"
                     /note="tail peptide"
                     /note="lassopeptide"
                     /prepeptide="tail"
                     /aSTool="lassopeptides"
                     /tool="antismash"
ORIGIN
        1 tcatctacgc atcgactgtt tctgccgaat cgatgcggtc aggtcgtgct gccgatgaat
       61 gagcttgttt atgccacaga ttggcataaa cgccctgttg cgccagaaac tccgatgcgt
      121 gccagtttcc acgatcgccc cctcttgcat gaccaagatg aggtctgcct gctgaatcgc
      181 ggcaagccgg tgtgcaatca cgagccgtgt ggtgtggcga taacgctcat agagatgttt
      241 gaagatatcc gcttcggtgt gtgcatcgag tgcggcagtc ggttcgtcca gcaacagtaa
      301 aggactattg cgcaataaag cgcgagctag ccccacacgt tgccgttcac cgcctgacaa
      361 tttgttgcct cgctcgccaa tcgacgtatc taggccctgg cttgccgccg cgatgaccgg
      421 tgttaacgcg caggtctgaa taacgctttc aagctccgag tcggaagctt gcggataggc
      481 aaaacaaatg ttttctcgga ttgagcgatc caacaaatgc gcctcttgtg tcacgtaacc
      541 gatcgactga cgcaattgcg attcggaaaa cgattcgatc ggttgacctt cgatgaggat
      601 ttgcccctca tcgagcttat ataggcgcag taacagtttg atgagcgtgg acttacccgc
      661 gccgctcgag cccaccagcg cacagaaagc gccggcagga atcgaaaaat tcgcgtggcg
      721 caatacccgt ctaccgcacg gatgacaaaa gctggcgttg cgaaattcga ttgacggcgc
      781 ctgctcgaac aaaggctgac ccaaggggtc gaagcgccat ctgacacgga tggtatgcag
      841 caattccgac agattgaata aaccgatctg gatgccccgc aacatatagc cgaacgtgct
      901 aagcggcagc gtgaatcgca gcaggtagct gttcaacagc acgaaatcgc cgatggtgag
      961 ccggtgcgcc aaaatatccc gtcccgccag cgccgtgagc gcagtcaacc cgataccgac
     1021 aatcagtaat tgcgcagcgc cgacgatttc catcttgcgc gcgatatgga tactcgccca
     1081 ggcccgcatt ttgaacacac ggttgagccg cgcgagttcg ctggcttcat tcgaaaacgc
     1141 tttgaccgtg tcggcatgcg tgagcgtatc gacgagtcgt gtcgaagcgc gatccgacgc
     1201 ccggtttttc ttgcgttgca gcacttcgca gcgcttcgcc gtcgcgacgc taaagtaaag
     1261 ataaagggtc aaaattccca gcaagactgc tgcataaacc cagccaaaca acgaacaaac
     1321 gacgatgccg acgaccacga tttcgacgca aatcggcgcg aggctccaaa agagtccgat
     1381 aattgcccgc atcgcactgt cttgtacgcg cgtgagctcg ctcacggagg cgcccgtttg
     1441 gtgagcactc aatgccgcag cgggctgacc gagcactcgg ctgacgtagt tcagcgtaat
     1501 cgtgcgtttg atccgctcat tgaccaccac gaacagatac tcttcgcaac ggtgcgtcgc
     1561 ttgcgacagg aaccatgccc cgccgtacgc tacgcagagc agataaacgc tcgtcgccga
     1621 gagcgacgcg ccggacaaac catcgatgag ccacttgagc agcatcggag agacaatggt
     1681 gagtccgacc gagcatccgg tacagccaat ggcggcgatg agctgaagac gcgttctcca
     1741 ttcgcgcgtc cacaaacaac ccagcagaaa aaagatcgtc ttaaaaagac cgtgccgcat
     1801 cgcgcctcac ccacaggtta ttgccaactt tgaataaaca gctcggcagc atagaggtga
     1861 aggaggtcca tgaaatgttt actgttcccc ttactgctct gctgaatcgc gtcatgggtg
     1921 cgcgctacgt cgatgtagcc tgctttggcc aaccatccct cgagacagac tgccatgaca
     1981 tgctcccggt ggtctcgaag acccaacaaa tcgatgcccg atgtctcgcc tttgttgcgt
     2041 cgccataggt tcgggtagcc ggtggcggcc gcgaccgatt ttctcagcac aatgcgattg
     2101 tgcgcccctt cgaaatgatc gtagctgggc gttgacagcg cgaactcgac catcggctgg
     2161 caaagaaacg gatagtgctt gggttgcgta aatggatagg caggaattct gacatcatcg
     2221 agcgataaaa aagccataaa cagctgatca agcttgccgg gtaggcgagt ctgggtgtgc
     2281 tgaaagaatg ggtgtaggca cgtccgtcga tcgaatagta tgtgctgcgt gtcctcggag
     2341 agtagcttaa aggtgtcgtt gaccttgtca gaaggatcgc tagcaaacac cgattgcacc
     2401 gcacgtttgg ccacgcgcat cagcggcgcg cggtacatga gtgccaaatc catcgccatg
     2461 cgcctaagtc gccgccattg cccggtgagt gccgcatcgg ccagcgcgct gcaaggcggc
     2521 ggcgccaaaa agagcgcatc gccgccatgc ccgctgatca gtggggtgtg ctcttgccgc
     2581 tcaaattgac tggcacaata ctgaccacgc gcgagcacgc tataccgcag actcggtttc
     2641 gccatgcgtg gcggctccga ctgaacagga gcaaacgtta aggccttccc attgacctcc
     2701 tggaggtgag cctgagtatg ctgtgccact tggcgggcat gatccagctc gttggacgaa
     2761 gccacatcgg cgtgataata gtgcgcgcaa gacagcggct gatctgccga ggcgacagcg
     2821 cgcatcgcca gcagcaccga actggattcc aggccgccgg agagctccag cacgggccga
     2881 tcactgaatg agacgaaccg gttcagataa tgctttaaaa tatcgaatgg ggtttgcgta
     2941 tcatccttga ttgcgtgctt gagcggatcc cagacggtca cgatctgcgg ctgacggccc
     3001 tccgtataag acagcgcgca acctaccgga agctctcgga tccgatcgaa cggcagctcg
     3061 ccactttgca tggtgcccgt acgtaaccag gtcgtaaaaa aggcgtgatt ccaggcgggc
     3121 cgctcgccgc gctccaccaa ggtttgatgc aaacacgtga tcgtatccga aaaaagcaag
     3181 ccgtcacggt tagaccaggc ccaatataac ggccattggt tactcggatc gctgcaacag
     3241 atcagctttt tggccgccag atcaaagctg ataaagagat atcttcccca gcattgcgcc
     3301 atgagacgcg ctgggccttc ggccaccatc gcttggagct cgccttcctg gatttcgcga
     3361 tggtcttgtt tcgaaaacgc cgaaccataa aagtagtagg cgctcgtgcc caagcgcccg
     3421 cataaaacgc gtgtggacgg ctcggattga tgccacagcg cgcccgcagt ccgatgggac
     3481 ggatcgcgca cgagcgtcca atcctgtttt tgtgcccaat catcctgcgc ttggggatag
     3541 gcgataccga gtagagtcat acggacaggt caagcagcgg atggggacat tccagaatta
     3601 cggcgagctc tttgcgtctg agcgggctat cgccaatcac cgccccgccg gcttccgacc
     3661 aggcgtgtgc ataaaacgga aaggattgca cgccaatcac caatttcagc gccaaaccgg
     3721 cacgcgcgca caacacggtc agcgcgacgg accactctaa acacttggtc gagcggggat
     3781 aaaacagaca agcccaattg agcgcgtcca cgaagggcgc ataatcatcg ggcagaccga
     3841 tacgcaggct ggcctcgcgg tgcgcctgat ggcacagttg caataaccca gccagtccct
     3901 gccgcgcggc gcatcggtgc acggatcgca agaggaacag cgctttgacg atgcgggtcc
     3961 aggcggggcg acgcgcgaac gcatcctcag gcaacttcca gccattggcc gacattccac
     4021 ccgttgtcgg agacgggctc aacatgttca gcttcgcgtc cgcctcggca aaggaagtga
     4081 cgctgatcca ctgcgcccgc tttaattctt ccagaataga ggccgactga accgcctcga
     4141 ctcgagcaaa gcgcgcatca cttagcccaa gctgctgggc caaggctgcc gattgccgct
     4201 cggtgagcac cgcataccga tcgcgcacca catcgagtaa aataatctgc tgagcaaaac
     4261 aggccccaaa tacatggggc atcaaatgat gcggcatgtg ggtatgatag ttgtatctcg
     4321 tggcgctcgg aaaacaccca gtaatggcct cgcatagctg actttgtctc caagcgccat
     4381 gctgcaccgc agacggaccg ttgctgggtg ccgacgttta tttattcgtc gtcggagtca
     4441 atgcgatcac tccagcggcc agcttccact tccttgtact gtcccgcacc gcccagcgtc
     4501 tctgacgacg cgcacagctc catcagcgat tcctcggcca acagcaggcc cgactcttgt
     4561 tgttgcttgt tcat
//
