LOCUS       BGC0000575              5596 bp    DNA     linear   BCT 10-AUG-2013
DEFINITION  Rhodococcus jostii lariatin biosynthetic gene cluster (larA, larB,
            larC, larD, larE), complete cds.
ACCESSION   BGC0000575
VERSION     BGC0000575
KEYWORDS    .
SOURCE      Rhodococcus jostii
  ORGANISM  Rhodococcus jostii
            Bacteria; Actinobacteria; Corynebacteriales; Nocardiaceae;
            Rhodococcus.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-07 19:54:53
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..5596
                     /country="China: Yunnan"
                     /db_xref="taxon:132919"
                     /isolation_source="soil"
                     /mol_type="genomic DNA"
                     /organism="Rhodococcus jostii"
                     /strain="K01-B0171"
     protocluster    1..5596
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[627:3163]"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      628..3163
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
     cand_cluster    1..5596
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /kind="single"
                     /product="lassopeptide"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..5596
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000575"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..5596
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lassopeptide"
                     /region_number="1"
                     /rules="(PF13471 and Asn_synthase or micJ25 or mcjC)"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            461..601
                     /gene="larA"
     CDS             461..601
                     /codon_start=1
                     /gene="larA"
                     /gene_functions="biosynthetic-additional (lassopeptides)
                     predicted lassopeptide"
                     /gene_kind="biosynthetic-additional"
                     /product="lariatin precursor protein"
                     /protein_id="BAL72546.1"
                     /transl_table=11
                     /translation="MTSQPSKKTYNAPSLVQRGKFARTTAGSQLVYREWVGHSNVIKPG
                     P"
     CDS_motif       461..538
                     /prepeptide="leader"
                     /note="peptide class: lassopeptide"
                     /note="predicted sequence: MTSQPSKKTYNAPSLVQRGKFARTTA"
                     /locus_tag="larA_lassopeptide"
                     /aSTool="lassopeptides"
                     /tool="antismash"
     CDS_motif       539..601
                     /prepeptide="core"
                     /locus_tag="larA_lassopeptide"
                     /peptide="lassopeptide"
                     /predicted_class="Class II"
                     /score="6.10"
                     /molecular_weight="2205.5"
                     /monoisotopic_mass="2204.1"
                     /core_sequence="GSQLVYREWVGHSNVIKPGP"
                     /aSTool="lassopeptides"
                     /tool="antismash"
                     /leader_sequence="MTSQPSKKTYNAPSLVQRGKFARTTA"
                     /leader_location="[460:538](+)"
     gene            628..2442
                     /gene="larB"
     CDS             628..2442
                     /codon_start=1
                     /gene="larB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Asn_synthase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177:asparagine synthase (glutamine-hydrolyzing)
                     (Score: 117.2; E-value: 1.3e-35)"
                     /gene_kind="biosynthetic"
                     /note="ATP/Mg2+-dependent amide formation"
                     /product="lariatin biosynthetic protein"
                     /protein_id="BAL72547.1"
                     /sec_met_domain="Asn_synthase (E-value: 1e-36, bitscore:
                     115.7, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNGIDIAVVTDDPAILKSVHERYPDGSKHSIELEHGHNVHIFVRT
                     ATLVLSSYIRDNEAIAVLGYSNVHESSMRAILESSPGVAHMNSALGQLIGAQWVVAIRK
                     GAVRIQGTVSGLSRVYWSKRGSRFVASNRSRELARLLGSELDPTQVAFRTIHPMQHPFT
                     SSSCWKDLEGVLPGEYLEVTGRSSPRTERWWTPATSYRSLEDGANETAAALFSVVRNQL
                     SDHSAASCDISGGLDSSSIAAIAANAAKSGETHTVLHGTTSVSRDEFNSDADWAIELSK
                     SLKLDSHSFLSWNDMPKEYDDLDALASYDLDEPSIASISHSRFTHLINVARSKGSQVHL
                     TGFGGDELFIGSPTFCVDLFKTQPLLSARLLLTYRAMYRWTFRSLVRPLTTPMSYQQWM
                     RTKSLSTDRSTLRIPPLSWGFHGVIPPWITRDARHSMYDHVRSASSATFPLAPTPGRHF
                     ELENLYQCARLFRTMSDIVSQTSGVLLVAPMLEQAVVEAAISVRTPERLTPHKYKPVLT
                     HATRGLLPAVVAERQTKGGEDTDAAIGFSENISAIRELWDESRLASLGIVDGDYLSNAL
                     RRPDSAEFDDCAIAKTLATELWLRSLEK"
     gene            2451..2705
                     /gene="larC"
     CDS             2451..2705
                     /codon_start=1
                     /gene="larC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RRE-containing: Stand_Alone_Lasso_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic"
                     /product="lariatin biosynthetic protein"
                     /protein_id="BAL72548.1"
                     /sec_met_domain="PF05402 (E-value: 2.9e-18, bitscore: 54.5,
                     seeds: 461, tool: rule-based-clusters)"
                     /sec_met_domain="Stand_Alone_Lasso_RRE (E-value: 1.3e-24,
                     bitscore: 74.6, seeds: 25, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVLRLRKNVIITPTEYGAVALDERSGDYYQLNSTAALILDQLTKK
                     IPVESIAARIALDFEVSKAQASADLDEYLRMLREQGLLR"
     gene            2720..3163
                     /gene="larD"
     CDS             2720..3163
                     /codon_start=1
                     /gene="larD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: PF13471"
                     /gene_kind="biosynthetic"
                     /note="similar to cycteine protease"
                     /product="lariatin biosynthetic protein"
                     /protein_id="BAL72549.1"
                     /sec_met_domain="PF13471 (E-value: 1.9e-25, bitscore: 77.7,
                     seeds: 84, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPVVGAMAIPSKTRISATERLRLASALSLGKALSHLPPGLLRRSM
                     TAFAAKARPASYREAEAAVVSITQYSKASAGPGSCLQRSISVCILMRLDGRWPTWCVGV
                     PSKPPFRAHAWIEAGGQIVAELGDMNSYSRLMTISTHAERTES"
     gene            3160..4938
                     /gene="larE"
     CDS             3160..4938
                     /codon_start=1
                     /gene="larE"
                     /gene_functions="transport (smcogs) SMCOG1288:ABC
                     transporter related protein (Score: 322.1; E-value:
                     1.1e-97)"
                     /gene_kind="transport"
                     /note="similar to ABC transporter"
                     /product="lariatin biosynthetic protein"
                     /protein_id="BAL72550.1"
                     /transl_table=11
                     /translation="MSGASPTPGAKKHVLRLFDGHWLAMASAMGLTIAAGGLAILQPSF
                     AGRIVDAVSNGSSITSAAAILVVALLGQILLDTVGHYQFERIGEKVVYKMRCAFALHVV
                     NLPVADIDGHRTGDLLSRASNDTAMIREIPRSIGDILFGAVSAGAAVVLMFSIDPVLVA
                     MVIAVIVVAFLGANQFLKRIQLAAGRRQVAVGEYASGLDRTLSAIRTVKLFRAEQREIR
                     SIVRSADHAYTAGVRTALLIAAGTPVVQLAITGCSLLILVVGGSRVARNELTLGELVSL
                     FMYTMYAVMPLSNLFGGLALIRTAMGSLERLLETLNRPVEGAALSRSASTGEHRPASST
                     SLVEFDRVSFAYGTSQVIDDVSFSIDRNQVTAIVGSSGAGKTSIISLACRFYEPTAGII
                     RWEGEEYQATPIGELRRHLALVEQDAPVMHGTIRENLQIANPESSDGEIWTALKHANLA
                     PEIECLPDGLDTVVLDHGRSLSGGQRQRLAVARALLSRAELILMDEPTAHLDRVNELEL
                     MQNLIEWRGARSLLVVAHRLSTVTRADRILVLDAGRIVATGTHLELVEDSAAYRRLIEQ
                     TLGAKPQGSELAPARA"
ORIGIN
        1 gcccgctgcc ggtgaccgag gccgttcggg ggcacatcgc cgcctcagtt caacggatct
       61 ggaccaacga gaaccggttg cactgttcac gatgccacct tcgccgaacc gtctgaacca
      121 tccgggcctg cttgtgcgac tttgccaatg ccctcccgct gcctcgcttt gcgcccgtgg
      181 cgacgccccc gacattgatc gcgaacaaag tcccccaaaa cgccgaaggt gtggttttat
      241 accgacaggc cggtttccta gcccgagggg ccgatccgag gggggctgaa tggcgttacg
      301 gcgtgttgcg acgactgagt ccacccgctg cgccatcagc agctttcctc tggcaccgcc
      361 gttcaattgc aagctcttgc tgatagttac ttgttgctaa tttgtccgca attagccccg
      421 ctaagtgatt ggcgggtgga tcaacggaag gcgaaatggc atgacctctc agccgagcaa
      481 gaagacgtac aatgcaccga gcctggtgca gcgggggaag ttcgcacgaa ccacagcagg
      541 ttctcagctg gtctatcgcg agtgggtcgg gcattcaaac gtcattaagc ccggccccta
      601 agctctctat gtgaaaagga tgcgctcatg aacggaatcg acatcgctgt cgttaccgac
      661 gatccagcta tcttgaaatc cgttcatgag cgttatccgg acggttcgaa acactccatc
      721 gagttggagc acggacacaa cgtccacatt ttcgtaagaa ccgccacact agttctaagc
      781 agttacatca gggacaatga ggcgatcgca gttctcggct actccaacgt ccatgaatca
      841 tccatgcgcg cgatccttga gtcgtcaccc ggggtggcgc acatgaactc cgcgttgggg
      901 caacttatcg gcgctcaatg ggttgttgcg atccgcaagg gtgcagtccg aatacaaggc
      961 accgtgagcg ggttgtcgcg cgtctactgg tcgaagcgcg gctcacgatt cgtcgcctcc
     1021 aaccggtcgc gtgagcttgc ccgactactc ggaagcgaac tggacccaac gcaggtcgcc
     1081 ttccgcacga ttcaccccat gcaacaccca ttcacctcct catcatgctg gaaggatctc
     1141 gaaggagttc ttccaggcga gtacctagaa gtgaccggcc ggtcgagtcc acggaccgag
     1201 cggtggtgga ctcccgcgac ctcctatcgc agcctcgagg acggtgcaaa cgaaacggcc
     1261 gcggcactat tttctgtagt aagaaatcag ctgagtgatc actcagccgc ttcctgcgac
     1321 atctccggcg gattggactc ctcatcgatc gcagcaattg ccgcaaacgc tgcgaaatca
     1381 ggcgaaacac ataccgtcct acatggaaca acgtccgtaa gccgagatga gttcaactcg
     1441 gatgccgact gggcgattga gctatcaaag tctctgaaac tcgacagcca tagctttctg
     1501 tcgtggaacg acatgcccaa agaatacgat gatctcgacg cactggcttc gtacgatttg
     1561 gatgagccat ccatcgcctc aattagccac agccggttta cccatctgat caatgtggcc
     1621 cggtccaagg gatcccaagt ccatctcaca ggattcggcg gagacgaact attcatcggc
     1681 tcgccaacgt tctgcgtcga tctattcaaa actcagcccc tcttatcagc tcgcctcctt
     1741 cttacatatc gagcaatgta ccgctggaca ttcagatcac tagtccggcc attaaccact
     1801 ccaatgagtt atcagcagtg gatgcgaacc aaatctcttt caacagatcg gtctacactt
     1861 cgcatccctc cgctcagctg gggatttcat ggcgtaattc caccctggat cacgagagat
     1921 gctcgtcatt ccatgtatga ccatgtgaga tcggcatcgt cagcgacttt cccgctcgct
     1981 cctactcctg ggcgtcattt cgagctggag aatctctatc aatgcgccag actgttccga
     2041 acgatgagcg atatcgtctc ccaaacaagc ggagtcctac ttgtcgctcc aatgctagag
     2101 caggcagtcg tcgaggctgc aataagcgtt cgaacccctg aacgactaac gccacacaag
     2161 tacaaacccg tccttactca tgcgacccgc ggacttctgc cagcagtagt ggccgaacgc
     2221 caaacaaagg gaggagaaga caccgatgct gccatcggat tctccgagaa catctcagca
     2281 atccgcgagt tatgggatga gtcgagactt gcttctcttg gaatcgtcga tggggactac
     2341 ctgagcaacg ctctacgcag gcccgatagc gcagaatttg acgactgcgc cattgccaag
     2401 acgctagcaa cagaattgtg gcttcggtcc ctggaaaagt aaagaggagc atggtgctac
     2461 gtttacgtaa gaatgtgatc atcacgccaa ctgagtatgg cgcagtggcc cttgatgaac
     2521 gttcgggtga ttattatcag ctcaatagca ccgcggcgtt gatacttgac caattgacaa
     2581 agaaaattcc cgtcgaatct attgctgcac ggatcgcgct ggacttcgag gtttcgaagg
     2641 cccaggcatc ggctgacctc gacgagtatc tcaggatgct ccgcgagcag ggtcttctgc
     2701 gatgaggaac gacatcggag tgccggttgt cggcgcaatg gcgattccga gcaagacaag
     2761 gatcagtgcc accgaaagac tcagactggc atcagctcta tcacttggaa aggccctaag
     2821 ccacctgccg ccaggcctgc tgcggcgcag catgaccgcg ttcgcggcaa aagcccggcc
     2881 tgcgtcgtat cgcgaagctg aggcggctgt cgtttcaatc acccagtaca gcaaggcttc
     2941 cgccggtcct gggagttgcc tgcagcgttc gatttcggtc tgcatcctga tgcgcctaga
     3001 cggcaggtgg cccacgtggt gcgtcggggt tccgagtaaa ccgccgttcc gtgcacatgc
     3061 ctggatcgag gccggtggtc agattgtcgc cgagttgggg gacatgaatt cctactctcg
     3121 cctcatgacg atctctactc atgcagagag gacggaatca tgagtggcgc ttcgcccaca
     3181 ccgggtgcca agaagcacgt tctccgactg ttcgatgggc actggctagc catggcctca
     3241 gctatgggac tgacgattgc ggctggaggt ctcgccatcc tgcagccgtc cttcgccggc
     3301 cgcattgtcg acgccgtatc gaacggatcc tcgatcactt ctgccgcggc gattctagtg
     3361 gtcgcactgc tcgggcagat cctcctcgac acggtcgggc actaccagtt tgagcgcata
     3421 ggcgagaaag tcgtctacaa gatgcgctgc gcgttcgcgc tccatgtcgt gaatctgcct
     3481 gtcgccgaca tcgacggtca ccgcacaggt gaccttctat ctcgggcgtc caatgacact
     3541 gcgatgattc gtgaaatccc gcggtccatc ggagacatct tgttcggagc tgtgtcggcg
     3601 ggcgctgcgg tcgtactgat gttctccatc gatcccgtcc tcgtcgcgat ggttatcgcc
     3661 gtaatcgttg tggctttcct cggtgccaac cagttcttga agcggatcca actcgcagct
     3721 ggccgtcggc aggttgccgt cggagaatac gcatcgggct tggaccgtac actgtcggca
     3781 attcgaacgg tcaagttgtt ccgtgcggaa caacgtgaga tccgatctat cgttcgatcc
     3841 gctgaccatg catatacagc cggtgtacgg actgcgctat taatcgcagc cggaactccg
     3901 gtggtgcaat tagctattac gggctgcagt ctgctcatac ttgtggttgg cggcagccgc
     3961 gttgcacgaa acgaactcac tttgggtgag ctcgtttcac tgttcatgta cacaatgtat
     4021 gcagtgatgc ctctctccaa cctcttcggc ggccttgcac tgattcgaac agccatggga
     4081 tcccttgaac gcttacttga gacgctgaac aggcccgttg aaggagccgc gctctcgcga
     4141 agtgcctcca ccggagaaca ccgccccgcg tcgagcacca gtttggtcga gttcgaccgt
     4201 gtgtcctttg cgtacggaac gagccaggtt atcgatgatg tctcgttctc catcgaccgc
     4261 aaccaggtca ccgcgatcgt cggctcctcc ggcgctggaa agacgagcat aatctcattg
     4321 gcatgccgat tttatgagcc cactgcagga attatccggt gggaaggcga agaataccag
     4381 gcgacaccaa ttggtgaact tcggcgacac cttgccttgg tggagcagga tgctccagtg
     4441 atgcatggaa caatccgtga gaatcttcag attgcgaatc cagaatcatc cgatggtgag
     4501 atatggaccg cactcaagca cgccaacctc gcaccggaga tcgaatgcct tcccgacggc
     4561 ctagataccg tcgttctgga tcacggcagg agtctgtccg gtggccagcg gcagcgcctt
     4621 gctgttgcac gcgcactgtt gtccagagcc gagttgatcc tgatggacga gcccaccgcg
     4681 cacctcgaca gggtcaatga gcttgagctt atgcagaacc taattgaatg gagaggggct
     4741 cgtagcctac tcgtggtcgc tcatcgactt tcgacagtga cccgcgccga tcgaatcctg
     4801 gtcttggacg ctgggcgcat cgtcgccacc ggcacacatt tggagcttgt cgaggactca
     4861 gcggcctatc ggcgccttat cgaacagact ttgggcgcca aaccacaagg ctccgaactc
     4921 gcgcctgccc gcgcctgact ggacaaggcg caggcaggtc ctatccggcg ggcgtcgaac
     4981 ctagccgact gacgccttcc cattcgttcg ggccacttga gcagcccgga tgatggcgca
     5041 gagggtgttc gggctcgatg cccgaacacc ctccataccc cttccggacg cggccccgga
     5101 actccgcact tccgggggcg cgccacgacg gtgactaggc gatgtcgatg tccgaggggc
     5161 gcagctcgcc gctgatcggg caacgcccgc cgatccaact tcccggacgg gctcaccggc
     5221 aacacatcga agcaccatca gcacatccgg aaccatgtac cgcggcagcg tcccaccgag
     5281 cacctcccgc accgactcga tttcgaccgc gcatccgccc tcgggcacca cataacccac
     5341 caattgctga ccgaaccccg cctcgtgcac caccaccacg gcccgcgcga tcgactcctg
     5401 cgccgccaac accgactcga tctcccccaa ctcgatccgc agaccccgca ccttcacctg
     5461 aaaatccatg cgccccaaaa actccagatc accgttcgag ttccaccgga cacgatcacc
     5521 ggtgcgatac aaccgcgaac ccacaccccc gaacggatcg gccacgaacc gctccgccgt
     5581 cagatcgacg cggccc
//
