LOCUS       BGC0000581              4496 bp    DNA     linear   BCT 26-JUL-2016
DEFINITION  Escherichia coli plasmid pTUC100 microcin J25 precursor (mcjA), McjB
            (mcjB), McjC (mcjC), and McjD (mcjD) genes, complete cds.
ACCESSION   BGC0000581
VERSION     BGC0000581
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 13:52:45
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4496
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4496
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[61:2535]"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      62..2535
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
     cand_cluster    1..4496
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /kind="single"
                     /product="lassopeptide"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..4496
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000581"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..4496
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lassopeptide"
                     /region_number="1"
                     /rules="(PF13471 and Asn_synthase or micJ25 or mcjC)"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(62..238)
                     /gene="mcjA"
     CDS             complement(62..238)
                     /codon_start=1
                     /gene="mcjA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: micJ25"
                     /gene_functions="biosynthetic-additional (lassopeptides)
                     predicted lassopeptide"
                     /gene_kind="biosynthetic"
                     /product="microcin J25 precursor"
                     /protein_id="AAD28494.1"
                     /sec_met_domain="micJ25 (E-value: 1.1e-28, bitscore: 87.0,
                     seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIKHFHFNKLSSGKKNNVPSPAKGVIQIKKSASQLTKGGAGHVPE
                     YFVGIGTPISFYG"
     CDS_motif       complement(128..238)
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence:
                     MIKHFHFNKLSSGKKNNVPSPAKGVIQIKKSASQLTK"
                     /locus_tag="mcjA_lassopeptide"
                     /aSTool="lassopeptides"
                     /tool="antismash"
     CDS_motif       complement(62..127)
                     /prepeptide="core"
                     /locus_tag="mcjA_lassopeptide"
                     /peptide="lassopeptide"
                     /predicted_class="Class II"
                     /score="5.40"
                     /molecular_weight="2107.3"
                     /monoisotopic_mass="2106.0"
                     /core_sequence="GGAGHVPEYFVGIGTPISFYG"
                     /aSTool="lassopeptides"
                     /tool="antismash"
                     /leader_sequence="MIKHFHFNKLSSGKKNNVPSPAKGVIQIKKSASQLTK"
                     /leader_location="[127:238](-)"
     gene            578..1204
                     /gene="mcjB"
     CDS             578..1204
                     /codon_start=1
                     /function="maturation of microcin J25"
                     /gene="mcjB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: PF13471"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lasso_Fused_RRE"
                     /gene_kind="biosynthetic"
                     /product="McjB"
                     /protein_id="AAD28495.1"
                     /sec_met_domain="Lasso_Fused_RRE (E-value: 1.3e-15,
                     bitscore: 45.5, seeds: 20, tool: rule-based-clusters)"
                     /sec_met_domain="PF13471 (E-value: 3.6e-25, bitscore: 76.5,
                     seeds: 84, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIRYCLTSYREDLVILDIINDSFSIVPDAGSLLKERDKLLKEFPQ
                     LSYFFDSEYHIGSVSRNSDTSFLEERWFLPEPDKTLYKCSLFKRFILLLKVFYYSWNIE
                     KKGMAWIFISNKKENRLYSLNEEHLIRKEISNLSIIFHLNIFKSDCLTYSYALKRILNS
                     RNIDAHLVIGVRTQPFYSHSWVEVGGQVINDAPNMRDKLSVIAEI"
     gene            1207..2535
                     /gene="mcjC"
     CDS             1207..2535
                     /codon_start=1
                     /function="maturation of microcin J25"
                     /gene="mcjC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Asn_synthase"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: mcjC"
                     /gene_kind="biosynthetic"
                     /product="McjC"
                     /protein_id="AAD28496.1"
                     /sec_met_domain="mcjC (E-value: 0.0, bitscore: 1073.8,
                     seeds: 3, tool: rule-based-clusters)"
                     /sec_met_domain="Asn_synthase (E-value: 4e-09, bitscore:
                     24.6, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEIFNVKLNDTSIRIIFCKTLSAFRTENTIVMLKGKAVSNGKPVS
                     TEEIARVVEEKGVSEVIENLDGVFCILIYHFNDLLIGKSIQSGPALFYCKKNMDIFVSD
                     KISDIKFLNPDMTFSLNITMAEHYLSGNRIATQESLITGIYKVNNGEFIKFNNQLKPVL
                     LRDEFSITKKNNSTIDSIIDNIEMMRDNRKIALLFSGGLDSALIFHTLKESGNKFCAYH
                     FFSDESDDSEKYFAKEYCSKYGVDFISVNKNINFNEKLYFNLNPNSPDEIPLIFEQTDE
                     EGEGQPPIDDDLLYLCGHGGDHIFGQNPSELFGIDAYRSHGLMFMHKKIVEFSNLKGKR
                     YKDIIFSNISAFINTSNGCSPAKQEHVSDMKLASAQFFATDYTGKINKLTPFLHKNIIQ
                     HYAGLPVFSLFNQHFDRYPVRYEAFQRFGSDIFWKKTKRSSSS"
     gene            2754..4496
                     /gene="mcjD"
     CDS             2754..4496
                     /codon_start=1
                     /function="confers immunity"
                     /gene="mcjD"
                     /gene_functions="transport (smcogs) SMCOG1288:ABC
                     transporter related protein (Score: 186.6; E-value:
                     1.3e-56)"
                     /gene_kind="transport"
                     /note="ABC transporter homolog"
                     /product="McjD"
                     /protein_id="AAD28497.1"
                     /transl_table=11
                     /translation="MERKQKNSLFNYIYSLMDVRGKFLFFSMLFITSLSSIIISISPLI
                     LAKITDLLSGSLSNFSYEYLVLLACLYMFCVISNKASVFLFMILQSSLRINMQKKMSLK
                     YLRELYNENITNLSKNNAGYTTQSLNQASNDIYILVRNVSQNILSPVIQLISTIVVVLS
                     TKDWFSAGVFFLYILVFVIFNTRLTGSLASLRKHSMDITLNSYSLLSDTVDNMIAAKKN
                     NALRLISERYEDALTQENNAQKKYWLLSSKVLLLNSLLAVILFGSVFIYNILGVLNGVV
                     SIGHFIMITSYIILLSTPVENIGALLSEIRQSMSSLAGFIQRHAENKATSPSIPFLNME
                     RKLNLSIRELSFSYSDDKKILNSVSLDLFTGKMYSLTGPSGSGKSTLVKIISGYYKNYF
                     GDIYLNDISLRNISDEDLNDAIYYLTQDDYIFMDTLRFNLRLANYDASENEIFKVLKLA
                     NLSVVNNEPVSLDTHLINRGNNYSGGQKQRISLARLFLRKPAIIIIDEATSALDYINES
                     EILSSIRTHFPDALIINISHRINLLECSDCVYVLNEGNIVASGHFRDLMVSNEYISGLA
                     SVTE"
ORIGIN
        1 aagcttctga tatgtgattt attgttgata gacaaaatga cagagttctt ctttcagaat
       61 atcagccata gaaagatata ggtgtaccaa tccccacaaa atactcaggc acatgtcctg
      121 caccaccttt tgtgagttgc gatgctgatt tttttatttg tataaccccc tttgcaggag
      181 atggaacatt atttttttta ccagaagaca gtttattaaa atgaaaatgc ttaatcattt
      241 ttacgttcct tatttgatgg aaatagtatg atgattttta cagaggaacc tcacgtcctt
      301 ctgttagaat tttattagca caaaatacaa ttgagatcaa taatcattac gctttagtaa
      361 tttatcaata aaatcattta gatacaaaca tccataaact aatcaatctg caaaagtggt
      421 caaatttagt acaatatttt ggatttttat acatttttct aattatttca gaatatttag
      481 ccatcaatta agaaaaaaat ttagcttgta gataaattca gaagttttat tattccaatt
      541 gagtgtaaag gcataactac aggagggagt gtgcaaaatg atccgttact gcttaaccag
      601 ttatagagag gatcttgtta tcctggatat aattaatgat agtttcagca tagtgcctga
      661 cgcaggtagc ttgctaaaag aaagagataa attgcttaaa gaattcccac aactatctta
      721 cttttttgac agtgaatatc atattggaag tgtttctcgt aatagtgaca cttcttttct
      781 tgaagaacgc tggtttctac cagaacctga caaaacatta tataagtgtt ctctatttaa
      841 acgatttata ttattactca aagtctttta ctatagctgg aatattgaaa aaaaagggat
      901 ggcatggatt ttcataagta ataaaaaaga gaataggcta tactccttga atgaagagca
      961 tcttatccgg aaagaaatta gtaatctttc cattatcttt catcttaata tttttaaatc
     1021 tgactgtctt acctattcat acgcactaaa aagaattctt aattccagaa atattgatgc
     1081 tcatcttgtt attggtgtaa ggacacaacc tttttatagc cactcttggg tggaggttgg
     1141 gggacaagtt atcaatgatg ctcccaatat gcgggataaa ttatctgtta ttgcagagat
     1201 atagttatgg aaatatttaa tgtcaagtta aatgatactt caattagaat tattttctgt
     1261 aaaacgcttt ctgccttccg gacagaaaat accatcgtta tgctcaaagg aaaagcagtt
     1321 tcaaatggca aacctgtatc cacagaggag attgccagag tagtggaaga aaaaggtgtt
     1381 tcagaagtaa tagaaaattt agatggtgtt ttctgtatcc taatttatca ttttaatgat
     1441 ctccttatag ggaaaagcat tcaatcaggc cccgctctat tttattgtaa aaagaatatg
     1501 gatatttttg tttcggataa aatttctgat atcaaatttt tgaatccaga tatgacattc
     1561 agtctaaata taacaatggc agaacattat ctgtcaggaa atcgaatagc aacccaggaa
     1621 tcactaatca ctggcattta caaagtaaat aatggtgagt ttataaaatt taataatcag
     1681 ttgaaacctg tgctacttcg tgatgagttt agtattacca aaaagaacaa ttcaactatc
     1741 gacagtatca ttgataatat tgagatgatg cgggataata gaaaaatagc cctattattt
     1801 tccggaggat tggattctgc attaattttt cacacactta aagaatcagg taacaaattc
     1861 tgcgcttatc attttttttc tgatgaatct gatgacagtg aaaagtattt tgctaaggaa
     1921 tactgttcaa aatatggagt tgattttata tctgttaata aaaacatcaa ctttaatgaa
     1981 aaactttatt tcaatttaaa tcctaatagt ccggacgaaa tccctttgat atttgaacag
     2041 acagatgaag aaggtgaagg tcagcccccc atagacgatg atttattata tctatgtggt
     2101 cacggtggag atcatatttt cggacaaaat ccttcagaac tttttggcat tgatgcatat
     2161 cgaagtcatg gcttgatgtt tatgcataaa aaaatagtag aattttccaa tctcaaggga
     2221 aagagatata aagatatcat attttcaaat atttccgcat tcattaatac atccaacgga
     2281 tgttctccag caaagcaaga gcacgtatca gatatgaaac ttgcctctgc tcagtttttt
     2341 gcaactgatt atacaggaaa aattaataaa ctaactccat tcctgcataa aaatattatc
     2401 cagcattatg ctggcttacc agtttttagt ctatttaacc agcactttga tcgttatccc
     2461 gttcgttatg aagcgtttca acgatttggt tcagatattt tctggaaaaa aaccaaacgg
     2521 tcatcttcat catgactaat attcagaatt ctatccggta aaaaggatga actagtgaat
     2581 acaataaaac agtcaggatt aattgaaata ttaggcatta accatattga attggaaagc
     2641 attttgtatg aaaatacgac tacacgtctg acaatggaac taccatatat acttaactta
     2701 taccgtctgg caaaattcat tcaacttcaa tccattgatt ataaaggtta attatggaaa
     2761 gaaaacagaa aaactcatta tttaattata tttattcatt aatggatgta agaggtaaat
     2821 ttttattctt ttccatgtta ttcattacat cattatcatc gataatcata tctatttcac
     2881 cattgattct tgcaaagatt acagatttac tgtctggctc attgtcaaat tttagttatg
     2941 aatatctggt tttacttgcc tgtttataca tgttttgcgt tatatctaat aaagcaagtg
     3001 tttttttatt tatgatactg caaagtagtc tacgtattaa catgcagaaa aaaatgtcgc
     3061 taaagtattt gagagaattg tataacgaaa atataactaa cttgagtaaa aataatgctg
     3121 gatatacaac gcaaagtctt aaccaggctt caaatgacat ttatattctt gtgagaaatg
     3181 tttcccagaa tatcctgtca cctgttatac aacttatttc cactattgtt gttgttttat
     3241 ctacgaagga ctggttttct gccggtgtgt tttttctcta tattctggta tttgtaattt
     3301 ttaataccag actgactggc agtttagcgt ctctcagaaa acacagcatg gatatcactc
     3361 ttaactctta tagtctgtta tctgatactg ttgataacat gatagcagct aaaaagaata
     3421 atgcattaag acttatttct gaacgttatg aagatgctct cactcaggaa aacaatgctc
     3481 agaaaaaata ctggttactc agttctaaag ttcttttatt gaactcttta cttgctgtaa
     3541 tattatttgg ttctgtattc atatataata ttttaggtgt gctgaatggt gtagttagta
     3601 tcggccactt cattatgatt acatcatata tcattcttct ttcaacgcca gtggaaaata
     3661 taggggcatt gctaagtgag atcaggcagt caatgtctag cctggcaggt tttattcaac
     3721 gtcatgccga gaataaagcc acatctcctt caataccttt tctcaacatg gagcgaaaat
     3781 taaacctgtc cataagagag ctttcattta gctatagtga tgataaaaaa atacttaatt
     3841 cagtcagtct tgaccttttt accggaaaaa tgtattcatt aaccggaccc agtggttcag
     3901 gaaaatccac ccttgtaaaa ataatatcag gttactataa aaattacttt ggagacattt
     3961 atctgaatga tatatcctta cgtaatatca gtgatgagga tttgaatgat gctatttact
     4021 acctaacaca agatgattat atttttatgg atacactacg atttaatctc cggctcgcaa
     4081 attacgacgc gtcagaaaat gaaatattta aagttcttaa actggcaaat ctttctgtcg
     4141 tcaacaatga accagtgagt ctggatacac accttataaa cagaggcaat aactattcag
     4201 gagggcaaaa acaacgaatt tcgttagcgc gactgttttt gagaaaacct gcaataatta
     4261 ttattgatga agccacatcg gctctggatt atattaatga atcagaaatt ttatcatcaa
     4321 taagaactca ttttcctgat gcgttaatta taaatattag tcaccgaata aatcttctgg
     4381 agtgttccga ttgtgtttat gtattgaatg aaggaaatat tgttgcttct ggccatttca
     4441 gggatttgat ggtcagcaat gaatacatat cgggactggc ttctgttact gaataa
//
