LOCUS       BGC0000585              6458 bp    DNA     linear   BCT 26-JUL-2016
DEFINITION  E.coli Plasmid pMccC7 mccA,B,C,D,E,F genes.
ACCESSION   BGC0000585
VERSION     BGC0000585
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
COMMENT     On Feb 25, 1995 this sequence version replaced gi:42441.
            ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-07 19:56:46
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..6458
                     /db_xref="taxon:562"
                     /mol_type="genomic DNA"
                     /organism="Escherichia coli"
                     /plasmid="pMccC7"
     protocluster    1..6458
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[300:1353]"
                     /cutoff="10000"
                     /detection_rule="minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /neighbourhood="10000"
                     /product="RRE-containing"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      301..1353
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /neighbourhood="10000"
                     /product="RRE-containing"
                     /protocluster_number="1"
     cand_cluster    1..6458
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /kind="single"
                     /product="RRE-containing"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..6458
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000585"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..6458
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RRE-containing"
                     /region_number="1"
                     /rules="minimum(1, [Bottromycin_Methyltransferase_RRE,
                     Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /subregion_numbers="1"
                     /tool="antismash"
     regulatory      161..166
                     /regulatory_class="minus_10_signal"
     gene            203..226
                     /gene="mccA"
     CDS             203..226
                     /codon_start=1
                     /db_xref="GOA:Q47505"
                     /db_xref="PDB:3H5R"
                     /db_xref="PDB:3H9J"
                     /db_xref="PDB:3H9Q"
                     /db_xref="UniProtKB/Swiss-Prot:Q47505"
                     /function="structure of microcin C7"
                     /gene="mccA"
                     /protein_id="CAA40808.1"
                     /transl_table=11
                     /translation="MRTGNAN"
     repeat_region   223..249
                     /note="palindrome"
                     /rpt_type=OTHER
     repeat_region   251..274
                     /note="palindrome"
                     /rpt_type=OTHER
     gene            301..1353
                     /gene="mccB"
     CDS             301..1353
                     /codon_start=1
                     /db_xref="GOA:Q47506"
                     /db_xref="InterPro:IPR000594"
                     /db_xref="InterPro:IPR009036"
                     /db_xref="InterPro:IPR016040"
                     /db_xref="PDB:3H5A"
                     /db_xref="PDB:3H5N"
                     /db_xref="PDB:3H5R"
                     /db_xref="PDB:3H9G"
                     /db_xref="PDB:3H9J"
                     /db_xref="PDB:3H9Q"
                     /db_xref="UniProtKB/TrEMBL:Q47506"
                     /function="production of microcin C7"
                     /gene="mccB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RRE-containing: Pantocin_Microcin_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ThiF"
                     /gene_kind="biosynthetic"
                     /protein_id="CAA40809.1"
                     /sec_met_domain="ThiF (E-value: 1.7e-48, bitscore: 154.0,
                     seeds: 54, tool: rule-based-clusters)"
                     /sec_met_domain="Pantocin_Microcin_RRE (E-value: 1.8e-21,
                     bitscore: 65.2, seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDYILGRYVKIARYGSGGLVGGGGKEQYVENLVLWENIIKTAYCF
                     ITPSSYTAALETANIPEKDFSNCFRFLKENFFIIPGEYNNSTENNRYSRNFLHYQSYGA
                     NPVLVQDKLKNAKVVILGCGGIGNHVSVILATSGIGEIILIDNDQIENTNLTRQVLFSE
                     DDVGKNKTEVIKRELLKRNSEISVSEIALNINDYTDLHKVPEADIWVVSADHPFNLINW
                     VNKYCVRANQPYINAGYVNDIAVFGPLYVPGKTGCYECQKVVADLYGSEKENIDHKIKL
                     INSRFKPATFAPVNNVAAALCAADVIKFIGKYSEPLSLNKRIGIWSDEIKIHSQNMGRS
                     PVCSVCGNRM"
     gene            1350..2564
                     /gene="mccC"
     CDS             1350..2564
                     /codon_start=1
                     /db_xref="GOA:Q47507"
                     /db_xref="InterPro:IPR011701"
                     /db_xref="InterPro:IPR016196"
                     /db_xref="UniProtKB/TrEMBL:Q47507"
                     /function="production of microcin C7"
                     /gene="mccC"
                     /note="valine start"
                     /protein_id="CAA40810.1"
                     /transl_table=11
                     /translation="MNTANILSVNYGAYNAIRMLIGVYHAAFLISTGVTISQLAVLQIV
                     FSVTVLILDFPLSVIADRFYRKQVVVSGVFFTILFYPLCIQSPDMLALVFSEIFYAIGI
                     CCISGAIEGWVLSALDGRNEKFTLCAHLCERVSSFGSVITGAVGILVVYFYDSYKSGYI
                     VSFFLMFVVFIVFLCVKTNGSGGEKEARDSIIKQAKESIGLITGSYNGTYFLSITCMFT
                     IGIQVIYHFWQPIMLSGESIGSLSKRELITLLMCHIGAFSMQYIANYLMPKIDVRCKLY
                     ILVCTLCSFLSSIACILLFMLITDNKVVLGVAIFSIIHGMISTIPVGAQNIFISRLNGN
                     YKNIISGALGLVSLSCRVASISVLGVISVLPGNMPVTTYIFISAIACVLCGCLFLMWSF
                     KKEMV"
     repeat_region   1520..1539
                     /gene="mccC"
                     /note="palindrome"
                     /rpt_type=OTHER
     gene            2561..3364
                     /gene="mccD"
     CDS             2561..3364
                     /codon_start=1
                     /db_xref="GOA:Q47508"
                     /db_xref="InterPro:IPR013216"
                     /db_xref="UniProtKB/TrEMBL:Q47508"
                     /function="production of microcin C7"
                     /gene="mccD"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089:methyltransferase (Score: 60.7; E-value:
                     2.4e-18)"
                     /gene_kind="biosynthetic-additional"
                     /protein_id="CAA40811.1"
                     /transl_table=11
                     /translation="MTIKHAEELTEEVILNMNYPDFVALMQQDNTPPGAEYTIDYWIKH
                     GCINKKSHLLDLACSTGFSSRECFKKEGASAEGIDISESAVMVANEKAKKLKANNLLKY
                     YVADACDLPFEDNSFTHVLGGCNFAFIQNRLIALNETHRCLNHMGSMCISNFYYRRKIS
                     DKLINDVYNAINFRPNPLWTLEYWHQFFSERFTLVSEENHEMESQSEDELKADIYDYIF
                     NRNEFTKGLNGSLQNVFFERFLKIRRPLNIQRDYQGVTLQIWRKK"
     repeat_region   2626..2643
                     /gene="mccD"
                     /note="palindrome"
                     /rpt_type=OTHER
     CDS             3349..3612
                     /codon_start=1
                     /db_xref="UniProtKB/TrEMBL:Q47509"
                     /note="unnamed protein product; ORF87"
                     /protein_id="CAA40812.1"
                     /transl_table=11
                     /translation="MAQKITPSKIVKHARELIIKGIESGENSFVIFDVDGALERLDHYR
                     CQLKSFFPNSSIAYSYKSNNLAQWCRLSQVKVCMQRCALLMK"
     gene            3552..5117
                     /gene="mccE"
     CDS             3552..5117
                     /codon_start=1
                     /db_xref="GOA:Q47510"
                     /db_xref="HSSP:1NSL"
                     /db_xref="InterPro:IPR000182"
                     /db_xref="InterPro:IPR009006"
                     /db_xref="InterPro:IPR016181"
                     /db_xref="InterPro:IPR022644"
                     /db_xref="UniProtKB/TrEMBL:Q47510"
                     /function="production and immunity"
                     /gene="mccE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Orn_Arg_deC_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1264:diaminopimelate decarboxylase (Score: 116.1;
                     E-value: 2e-35)"
                     /gene_kind="biosynthetic-additional"
                     /protein_id="CAA40813.1"
                     /sec_met_domain="Orn_Arg_deC_N (E-value: 4.7e-19, bitscore:
                     57.6, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVQIISGKGLYAEVCSVDEMNLAKRDGFKRIVFDGPLKKTSELLK
                     AIEIGALIEVDNIDECKRLNELCKLHKLTCRIHLRLSHYYDDNLSRFGLSESEAINLLE
                     MLISKSEYLILDGFHLHVGSNLPNAEKICKAIIQYHELILRYMPDDGTLNLGSGIPADS
                     FSASSDNPTPCPEVFFSSIYDTIKNCFGTVCDKWNYIFEPGRHLVEDFGYFIGKVISTK
                     NRYGVKVAQTNIGINWIPSIRNWDHSFTLFHNHNHISDDKSDEYIIAGFNCFECDCLFP
                     SVILPSNLSDYLFSVRGCGAYDMQTGNQWTRNLYAVYTITNDVVNISRIHRRELDFRKY
                     DVSLTPSGIKVNDEITLLYPALKYAEELYLLINQNKINFIKSMAWPAFVNNISDSVSFI
                     EQSMIDNQNEKALILFIKYKTKIAGVVSFNIIDHANKTAYIGYWLGANFQGKGIVTNAI
                     NKLIQEYGDSGVIKRFVIKCIVDNKKSNATALRCGFTLEGVLQKAEILNGVSYDQNIYS
                     KVIG"
     repeat_region   3586..3600
                     /gene="mccE"
                     /note="palindrome"
                     /rpt_type=OTHER
     repeat_region   5047..5063
                     /gene="mccE"
                     /note="palindrome"
                     /rpt_type=OTHER
     repeat_region   5127..5142
                     /note="palindrome"
                     /rpt_type=OTHER
     gene            complement(5173..6207)
                     /gene="mccF"
     CDS             complement(5173..6207)
                     /codon_start=1
                     /db_xref="GOA:Q47511"
                     /db_xref="InterPro:IPR003507"
                     /db_xref="UniProtKB/Swiss-Prot:Q47511"
                     /function="immunity"
                     /gene="mccF"
                     /protein_id="CAA40814.1"
                     /transl_table=11
                     /translation="MMIQSHPLLAAPLAVGDTIGFFSSSAPATVTAKNRFFRGVEFLQR
                     KGFKLVSGKLTGKTDFYRSGTIKERAQEFNELVYNPDITCIMSTIGGDNSNSLLPFLDY
                     DAIIANPKIIIGYSDTTALLAGIYAKTGLITFYGPALIPSFGEHPPLVDITYESFIKIL
                     TRKQSGIYTYTLPEKWSDESINWNENKILRPKKLYKNNCAFYGSGKVEGRVIGGNLNTL
                     TGIWGSEWMPEILNGDILFIEDSRKSIATIERLFSMLKLNRVFDKVSAIILGKHELFDC
                     AGSKRRPYEVLTEVLDGKQIPVLDGFDCSHTHPMLTLPLGVKLAIDFDNKNISITEQYL
                     STEK"
ORIGIN
        1 gatgattata ttctgtatgg ttatttatgt ctaaatatat aaattaataa attttagaat
       61 aaaaatgtag aatcgcaaat aatatgattg tgctgttctt tttttgtgaa ataaatctat
      121 gtttgtatat ttttataagg aatattgttt tttatagtga taaaaacact tgtgaattaa
      181 tcacagtaat aggaggcgta aaatgcgtac tggtaatgca aactaatgga aaaatataaa
      241 tgtccattaa atgccaccct gtacagggtg gcatacaaaa gttatctgcg aggtttattt
      301 atggattata tattgggtcg ctatgtcaaa attgctcgat acgggagcgg tggattagtt
      361 ggtggaggcg gaaaggagca atatgttgag aatttagtgc tttgggaaaa tattataaaa
      421 accgcatatt gtttcataac cccatcgagt tatactgcag cgttggaaac ggccaatata
      481 cctgaaaaag atttctctaa ttgctttagg ttccttaagg aaaatttctt tattatacct
      541 ggcgagtata ataactctac tgagaataat cgttactctc gtaacttttt acattatcaa
      601 agttatggtg ctaatcctgt tcttgtgcaa gataaactaa aaaatgctaa ggtagtaatt
      661 cttggttgtg ggggtatagg aaatcatgtg tctgttatac tcgcaacgtc tggtattggc
      721 gaaattatac taattgataa tgatcaaata gaaaatacaa atttgacaag gcaggtttta
      781 ttttcagagg atgatgtcgg taaaaataaa acagaagtta taaaacgcga gttattaaaa
      841 agaaactctg aaatttcggt gtctgaaatc gctttgaata taaacgatta cactgatctt
      901 cataaggtac cagaagctga tatatgggtt gtttctgcag atcatccatt taatctgatt
      961 aactgggtta ataaatattg cgttagagca aatcagccct atataaatgc tgggtatgtt
     1021 aatgatattg cagtctttgg tccattatat gttcctggta aaacaggatg ttatgagtgc
     1081 caaaaagtcg tggcagatct atatggttca gaaaaggaaa acattgatca taagattaag
     1141 ctaatcaata gcagatttaa accggcaaca tttgcacctg tgaataatgt tgccgctgca
     1201 ttatgtgcgg cagacgtaat taagttcatt ggaaaatact ccgagccatt atcacttaat
     1261 aaaagaatag gcatctggtc agatgaaata aaaatacatt cccaaaatat gggccgttcg
     1321 cctgtttgct ctgtgtgtgg aaatagaatg tgaatactgc aaatatttta tctgtgaact
     1381 atggagctta taatgctata cgtatgttaa ttggtgtcta ccatgcagca tttcttatat
     1441 ctacaggtgt cactatatct caacttgctg ttttacagat cgttttttct gtgacagtgc
     1501 tgattcttga ttttccttta tctgtcattg cagacagatt ttacaggaaa caggttgtag
     1561 ttagtggcgt attttttact atattatttt accctctttg tattcagtct cctgatatgc
     1621 tggcattggt tttttctgaa atattttatg ccattggtat ttgttgtata tctggggcta
     1681 tagagggatg ggtgttaagt gctctggatg gtagaaatga aaagtttacc ttatgtgctc
     1741 atttatgcga aagggtatct tcctttggga gtgtgataac tggtgctgtt ggaatattag
     1801 ttgtctactt ctatgattct tataaatctg gctatatagt atcgttcttc ttgatgtttg
     1861 ttgtctttat cgttttttta tgtgtgaaaa ctaatggcag tggaggtgaa aaagaagcac
     1921 gagatagtat tattaaacag gcaaaagaga gtattggatt gataacagga agttataatg
     1981 ggacatattt tttatctata acatgtatgt ttacaattgg tatacaggta atttatcatt
     2041 tttggcagcc aattatgcta tctggggaaa gtattggctc attatcaaaa agagagctta
     2101 taactttact gatgtgtcat attggtgcat tttcaatgca atatatagca aactatctta
     2161 tgccaaagat agatgtaagg tgtaaacttt atatacttgt ttgtacatta tgttcatttc
     2221 ttagttcaat cgcctgtatt ttactattta tgttaataac agataataag gttgttctcg
     2281 gcgtagctat tttctctata atccatggga tgatttcaac aatacctgtt ggagcacaga
     2341 atatattcat aagcagactt aatggcaatt ataagaatat tatttcggga gctcttggtc
     2401 ttgtttctct ttcatgtcgt gttgcttcca tttctgtgtt gggagttata tctgtattac
     2461 caggtaatat gcctgtcacc acttatatat ttatctctgc aattgcatgt gtactgtgcg
     2521 gttgtttgtt cttaatgtgg tcctttaaaa aggagatggt atgacgatta aacatgctga
     2581 agaattgacg gaagaagtta ttttaaatat gaattaccct gattttgttg ctctgatgca
     2641 acaggataac actcctcctg gagcagagta caccatagac tactggatca agcatggatg
     2701 tattaacaaa aagtctcacc tattagattt agcatgttcc acgggtttta gttcacgtga
     2761 atgcttcaaa aaagaaggtg catctgcaga gggtattgat atatctgaat cagctgtaat
     2821 ggtcgcaaat gaaaaggcaa aaaagcttaa ggctaataat ttattgaaat attatgtagc
     2881 tgatgcttgt gatttaccat ttgaagataa ctcttttaca catgttctcg gtggatgtaa
     2941 ttttgcattt atacaaaatc ggttaatagc tttgaatgaa actcatcgat gtttaaacca
     3001 catgggaagc atgtgcattt caaattttta ttacaggcgt aaaatatcag ataaacttat
     3061 caatgatgta tataatgcaa taaatttcag acccaatcct ttgtggactt tggaatactg
     3121 gcatcagttt ttttctgaaa gatttactct tgtttcagaa gaaaaccatg agatggaatc
     3181 tcaaagtgaa gatgagttaa aagccgatat ttatgattac attttcaata gaaatgagtt
     3241 tacaaaaggt ttgaatggtt cacttcagaa tgtatttttt gaaagatttt taaaaatacg
     3301 cagaccatta aatatccaaa gagattatca gggagttaca ttgcaaatat ggcgcaaaaa
     3361 ataacaccat ctaaaattgt taaacatgcc cgggagttaa ttattaaggg gattgaatcg
     3421 ggcgaaaact catttgtcat ttttgatgta gatggagctc tggagcgtct ggatcattac
     3481 aggtgtcaac ttaagagttt tttccccaac agtagtatcg catattcata taagtcaaat
     3541 aatctggcac aatggtgcag attatctcag gtaaaggttt gtatgcagag gtgtgctctg
     3601 ttgatgaaat gaacctcgcc aaaagagacg gttttaagcg gattgttttt gatggccctt
     3661 taaaaaaaac gagtgaacta ttaaaggcaa tagagattgg ggcattaatt gaagttgaca
     3721 atattgatga atgtaaaaga cttaatgaac tatgtaagtt gcataagctg acatgtagaa
     3781 ttcatttgag attatcgcat tactatgatg ataatttatc aagatttggc ttatctgaaa
     3841 gtgaggctat taatttactg gagatgttga tcagtaagtc agagtatctc attttagacg
     3901 ggttccattt acatgttggc tctaatctac ccaatgcgga aaaaatatgt aaagctatta
     3961 tacagtatca tgagttgatt cttaggtata tgccagatga tggcactctt aatcttggta
     4021 gtgggatacc tgcagattct ttctcagcat ccagtgataa cccaactcca tgtccggagg
     4081 tatttttctc atcgatatat gatacaataa aaaattgctt cggtactgta tgtgataaat
     4141 ggaactatat ttttgaacca gggagacatc tggtcgaaga ctttggctac tttataggga
     4201 aagttattag tactaaaaac aggtatggtg ttaaagttgc tcaaactaac attggtataa
     4261 actggatacc ttcgatcaga aattgggacc attcatttac attgtttcat aatcataacc
     4321 atatttcaga tgataaatct gatgagtata ttattgctgg attcaattgc tttgaatgtg
     4381 attgcttgtt tccttcagtt attcttccca gtaacttatc tgattattta ttttctgttc
     4441 gagggtgtgg tgcttatgat atgcaaacag gaaaccagtg gacgcgtaat ctttatgctg
     4501 tatataccat aacaaatgat gttgttaata tatcaaggat tcacagaaga gaacttgatt
     4561 tccggaagta tgatgtatct cttacgccat caggaataaa agtaaatgat gaaattacat
     4621 tattgtatcc agcgttaaaa tacgctgaag agttatattt gctcattaat caaaacaaaa
     4681 taaatttcat taaaagcatg gcatggccag cgtttgttaa taacatttct gattctgttt
     4741 ctttcattga gcaatcaatg attgataacc agaatgaaaa agcattaatc ctctttatta
     4801 aatacaaaac taagatagct ggcgttgtat cgtttaatat tattgaccat gccaataaaa
     4861 cagcatatat tggctattgg ttaggtgcta actttcaggg gaaggggatt gtaaccaatg
     4921 ctataaataa actgatacag gagtatgggg attcgggcgt tataaaaaga tttgttataa
     4981 aatgtattgt tgataataaa aaaagtaatg ccacggcatt gaggtgtggc ttcaccttag
     5041 aaggtgttct gcaaaaagca gaaatactca acggtgtatc atacgatcaa aatatttatt
     5101 caaaagtaat tggttaatac tgtatggcgt agtattctac gcattttgtt tatgtctgtg
     5161 agatgataat atttatttct cggtagatag atattgttct gttatagata tatttttgtt
     5221 gtcaaagtca atagctaatt ttacaccaag tggaagagtt agcattggat gtgtatgtga
     5281 acaatcaaat ccatccagta caggaatctg tttcccatct aatacctctg ttaatacttc
     5341 atatggtctg cgtttacttc ctgcacaatc aaaaagctca tgtttcccga gtattattgc
     5401 actaacttta tcaaacacgc gattaagctt tagcatagag aataatcgtt caattgttgc
     5461 aatgcttttc cgactgtcct caataaacaa tatatctcca ttaagaattt caggcatcca
     5521 ttcactcccc catatacctg tcaaagtatt tagatttcct ccaattacac gcccctcaac
     5581 ttttccggaa ccataaaagg cacagttgtt tttatatagc ttcttaggcc ttaatatctt
     5641 gttttcattc cagtttatgc tctcatcact ccacttttca ggtaatgtgt aggtatatat
     5701 tcctgattgt tttcttgtta gtattttaat aaatgattca tatgttatat ccacaagagg
     5761 tggatgttca ccaaacgaag gaataagagc tggtccatag aatgttatta accctgtttt
     5821 tgcatatatt cctgctaata aagcagttgt atctgagtaa cctatgataa ttttggggtt
     5881 tgcaatgata gcatcatagt ccagaaacgg tagtagtgaa ttactgttat ctccaccgat
     5941 cgttgacatt atacaggtaa tatcaggatt gtagactaac tcattaaatt cttgagctct
     6001 ttctttaata gtacctgaac gataaaaatc tgttttaccg gtaagcttcc ctgataccag
     6061 cttaaatccc tttctctgaa gaaactcaac tccccgaaaa aaacgatttt ttgcagtaac
     6121 tgttgccgga gcagatgatg aaaagaaacc aattgtatct cctactgcca ggggagcggc
     6181 cagtagtgga tgagattgta tcatcatgag gatttttcct ttccatttag tgtcaaaatg
     6241 aacccgtttt gagatatgtc agtagcactt tcagtttatg ggcttttttt gaaaatcgca
     6301 gtgttctctc gtaaattttc tcgtccaatc ccccccgaaa agcctcagat ttcacgtagc
     6361 gcaccgccac tactgtatcc atcaaaaaaa ctgatagctt ttttaatcat ctgttgtgct
     6421 gcaacttact gaaaaaaatg gattcatgaa aacgccga
//
