LOCUS       BGC0000586             13741 bp    DNA     linear   BCT 31-JUL-2008
DEFINITION  Klebsiella pneumoniae strain RYC492 microcin E492 production gene
            cluster, complete sequence.
ACCESSION   BGC0000586
VERSION     BGC0000586
KEYWORDS    .
SOURCE      Klebsiella pneumoniae RYC492
  ORGANISM  Klebsiella pneumoniae RYC492
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
COMMENT     On Jul 31, 2008 this sequence version replaced AF063590.2.
            ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2024-10-17 13:53:08
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 13741
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..13741
                     /clone="J"
                     /db_xref="taxon:1250520"
                     /mol_type="genomic DNA"
                     /organism="Klebsiella pneumoniae RYC492"
                     /strain="RYC492"
     subregion       1..13741
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="ribosomal"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..13741
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(255..554)
                     /gene="mceA"
     CDS             complement(255..554)
                     /codon_start=1
                     /function="channel-forming bacteriocin"
                     /gene="mceA"
                     /note="MceA; overlapping with mceB"
                     /product="microcin E492 precursor"
                     /protein_id="AAD04332.2"
                     /transl_table=11
                     /translation="MREISQKDLNLAFGAGETDPNTQLLNDLGNNMAWGAALGAPGGLG
                     SAALGAAGGALQTVGQGLIDHGPVNVPIPVLIGPSWNGSGSGYNSATSSSGSGS"
     misc_feature    255..13092
                     /note="microcin E492 production gene cluster"
     mat_peptide     complement(258..509)
                     /gene="mceA"
                     /product="microcin E492"
     sig_peptide     complement(510..554)
                     /gene="mceA"
     gene            complement(541..828)
                     /gene="mceB"
     CDS             complement(541..828)
                     /codon_start=1
                     /gene="mceB"
                     /note="MceB; overlapping with mceA"
                     /product="microcin E492 immunity protein"
                     /protein_id="AAD04333.1"
                     /transl_table=11
                     /translation="MTLLSFGFSPVFFSVMAFCIISRSKFYPQRTRNKVIVLILLTFFI
                     CFLYPLTKVYLVGSYGIFDKFYLFCFISTLIAIAINVVILTINGAKNERN"
     gene            1306..2418
                     /gene="mceC"
     CDS             1306..2418
                     /codon_start=1
                     /function="required for microcin E492 modification"
                     /gene="mceC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) MGT"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1062:glycosyltransferase, MGT family (Score: 251.7;
                     E-value: 2.4e-76)"
                     /gene_kind="biosynthetic-additional"
                     /note="enterochelin glycosyl transferase; similar to IroB"
                     /product="MceC"
                     /protein_id="AAL08396.1"
                     /sec_met_domain="MGT (E-value: 4.8e-112, bitscore: 365.0,
                     seeds: 193, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRILFIGPPLYGLLYPVISLAQAFRVNGHEVVVASAGKFTHKAAD
                     AGLVVFDAAPGLDSEAGYRHQEELRKKSNTVGHFSFFSDEMADRLVDFAGQWHPDLIVY
                     PPLGLAGPLIAAKYGIPSVMQTVGFAHTSAHIQMVTRSLGNAYRRHGVSGPPRDLAWID
                     VTPPSMSILKNDGEPVISMRYVPYNGGAVREPWWDRAPGRKRLLISLGTVKPMVDGLDL
                     ISWVMDSANEVEADIILQLAMNARAGLRKLPSNVRLVEWIPMGVFLNGADGFIHHGGAG
                     NTLTALYNGIPQIVFGEGADRPVNAKAVAERGCGIIPGDHGLTSDLVNTFLYDDSLRLC
                     SAQVAAEMAEQPSPAEIAGVLVAKLKTIWQ"
     gene            complement(2597..3841)
                     /gene="mceD"
     CDS             complement(2597..3841)
                     /codon_start=1
                     /function="apo and ferric salmochelin esterase"
                     /gene="mceD"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1098:enterobactin/ferric enterobactin esterase (Score:
                     243.2; E-value: 9e-74)"
                     /gene_kind="biosynthetic-additional"
                     /note="similar to IroD"
                     /product="MceD"
                     /protein_id="AAL08397.1"
                     /transl_table=11
                     /translation="MPFMRNHPSRSLDDICTESRICAQLSTNPSMDITHFWREVESAAL
                     PLMTKSSSNTENREVTFLWRSEEVLQGVYLRLNRVTDRRDVKKGLMTHIPSTNIWTLTL
                     ELPASYRGSYSFIEIPPEMPQEDISQLGGRFSPLPGQPDPFNKTSEINIRGFGESVLSL
                     DLAPGQTEWDDTSHTCSGVLSTSYSFVAGHQRQIRLYFPGYPTSVPLGLLVLPDAETWF
                     DRIGITRAIDIAITTGRIAPMAILGIDNINESDRVNILGGNKELILDIAGNLIPRLYKD
                     YPDIVWAGRSNTILAGQSLGGVTALMAALYAPDIFGTVISHSPSMWWSPDRNTPLMFTE
                     NDTSWVSKQVLSALPKDVNIQMGVGSLEGATVPHVQQLHQSLLASGLKSKLSVYTGGHD
                     YAWWRGAIIDGLSDV"
     CDS             complement(3918..4085)
                     /codon_start=1
                     /note="similar to MchS2, microcin I47 precursor"
                     /product="MceS2"
                     /protein_id="ACF93943.1"
                     /transl_table=11
                     /translation="MREIMGSRLNFVKEWLNFDGLRGSANITNQRGTGTGSYIDANGAC
                     RGPGTPTSGM"
     CDS             complement(4315..4521)
                     /codon_start=1
                     /note="similar to MchS3, microcin I47 immunity protein"
                     /product="MceS3"
                     /protein_id="ACF93944.1"
                     /transl_table=11
                     /translation="MITNILVAKKIMVTFIVILTLSAHSSEIKTIKQGPEKEPYFPIGM
                     NTLVGQQKEGKKTQPHSSVRQSQ"
     CDS             complement(4683..4940)
                     /codon_start=1
                     /product="MceM"
                     /protein_id="ACF93945.1"
                     /transl_table=11
                     /translation="MIFLYLDKIPLFILGIGLLTSFALPGSSALDSPKFLCIYSSTILA
                     GISFIYQVFRHGTNTEFFLAMLITVSFVVMLPVIKMHFAY"
     CDS             complement(4962..5231)
                     /codon_start=1
                     /function="putative bacteriocin"
                     /note="N- and C-termini similar to the microcin E492, I47,
                     H47 and M precursors MceA, MchS2, MchB and McmA"
                     /product="MceL"
                     /protein_id="ACF93946.1"
                     /transl_table=11
                     /translation="MRALTENDFFAVSGADRGDAAVAGAVAGGTAGAAAGGWAGAQMGA
                     TVGSLAGPVGTVVGFVAGAAAGRYGGAFIYDSFSSPSNSSSSGS"
     gene            complement(5371..5715)
                     /gene="mceE"
     CDS             complement(5371..5715)
                     /codon_start=1
                     /gene="mceE"
                     /note="C-terminus similar to MchS4, which promotes
                     enterochelin production"
                     /product="MceE"
                     /protein_id="AAL08398.1"
                     /transl_table=11
                     /translation="MNGNKAHKKTKNISINNVNEVQHQQVTEVSKEDGDFLTLLYSPQP
                     LPSPENTALKANYSIRTGNTVSEYQDIADTECNQHIMQIDSAQDKREYGDDNYICGCGC
                     ESCCGCGHDL"
     CDS             5936..6043
                     /codon_start=1
                     /note="similar to MshX"
                     /product="MceX"
                     /protein_id="ACF93947.1"
                     /transl_table=11
                     /translation="MVFATDLVAADDGLRTTLLALIMTATLLEYSLSMF"
     gene            6182..7756
                     /gene="mceJ"
     CDS             6182..7756
                     /codon_start=1
                     /function="required for microcin E492 modification with
                     salmochelin"
                     /gene="mceJ"
                     /product="MceJ"
                     /protein_id="AAL08403.1"
                     /transl_table=11
                     /translation="MVTEKYSQMNQLCSLAELNDNLVPFTARRITSSLIWCAENVRDTE
                     VLLKACSYIIDPVSSASAKTFHAERYGGSGIQRNGGGARCGFDGNYQVKGMGANPLVGV
                     GTDGRHSNGALGAIHAIYEALWGEVLAQILPYSAVRVQAVLLTNLYTDKAFDRSHGKSR
                     RALLVRDPVVRPAHFERAPYFRAKPEYAGQLIHDARRVRSVIRMLPSNLPVPSEGFSGE
                     ARRNPRIYCIEGLCELARREAWQMAFCRTRFLRLTTSPSNIAMDGRLMDFNGLSCLFPG
                     DYPDNFGHQFRLAELVKEPMVLIQGLSDLCLYIGKYMFDPDFTLVSRLKVEETFQKTFH
                     EACYYCYLEQLGIPTEFLPQEGIPDTLKQLVNSLVVLVNKRSDRLCCPDTACGDDSPLQ
                     SLVVELIRQSQAQTHPLDNDAEHDVHFTEAQLCFSRAIHWLTQDSIRRQINVSSLLKEM
                     ENHARKRLQPRKWLGKACLSEEIASLLDEHSDNPYYLREAFSDMGTRMQAFSREAFGHV
                     NPVRIAV"
     gene            7769..8260
                     /gene="mceI"
     CDS             7769..8260
                     /codon_start=1
                     /function="required for microcin E492 modification with
                     salmochelin"
                     /gene="mceI"
                     /note="similar to toxin-activating protein C, leukotoxin C"
                     /product="MceI"
                     /protein_id="AAL08402.1"
                     /transl_table=11
                     /translation="MTMMSHIRESIRGREEWTVYEQIGFAVSCMLHNRNYSLYPVLTIQ
                     YWTEYAIQHNQIKFLFDSRGFPLAYITWAYLEADTEARLLNDPEFRLHPSEWDEDGRIW
                     ILDFCCKPGFGRKAIERLFQLRPWGEGEVRWLSRRKKIMTFRPMRRHKMPMAPETQKEL
                     "
     gene            8419..9660
                     /gene="mceH"
     CDS             8419..9660
                     /codon_start=1
                     /function="second component of microcin E492 exporter"
                     /gene="mceH"
                     /gene_functions="transport (smcogs) SMCOG1029:RND family
                     efflux transporter MFP subunit (Score: 77.4; E-value:
                     1.6e-23)"
                     /gene_kind="transport"
                     /note="membrane-fusion protein; similar to CvaA, the second
                     component of colicin V exporter"
                     /product="MceH"
                     /protein_id="AAL08401.1"
                     /transl_table=11
                     /translation="MKWRGRAILLPGIPLWLTMLGSIAFITAFLTFVIAGTYSRRVNVS
                     GEVTTWPRAVNIYSGVQGFVVRQFVHEGQSIKKGDPIYQIDVSKSTRSGVVTDNQRRDI
                     ENQLVRVDNIISRLEESKKITLNTLEKQHLQYSDAFRRSSDIIRRAEEGIKIMKNNMEN
                     YRNYQLKGLINKDQLTNQVALYYQQQNNLLSLSGQNEQNALQITSLESQIQTQAADFDN
                     RIYQMELQRYELQKELIDTDVGGEIIIRALSDGKVDSLSVTVGQMVNAGDSLLQVIPEN
                     IENYYLILWVPNDAVPYISAGDKVNIRYEAFPAEKFGQFSATVKTISRTPASTQEMLTY
                     KGAPQNTLGTSVPWYKVIVKPEKQIISYGGKSLPLENGMKAESTLFLEKRRIYQWMLSP
                     FYDMKHSATGPIDE"
     gene            9653..11749
                     /gene="mceG"
     CDS             9653..11749
                     /codon_start=1
                     /function="ABC protein of microcin E492 exporter"
                     /function="microcin leader peptide processing"
                     /gene="mceG"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288:ABC
                     transporter related protein (Score: 245.3; E-value:
                     2.2e-74)"
                     /gene_kind="transport"
                     /note="similar to CvaB, an ABC exporter of colicin V"
                     /product="MceG"
                     /protein_id="AAL08400.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 6.8e-42, bitscore:
                     132.6, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSNGNVRRMINQLDMRWRRRVPVIHQTETSECGLACLAMICGHFG
                     KNIDLISLRRKFNLSARGANLAGINSIAEQLGMVTRALSLELDELGALKTPCILHWDFS
                     HFVVLVSVKRNRYVLHDPARGRRNVGLEEMTRYFTGVALEVWPGSEFSEETTQNRIQLR
                     SLINSIYGIKSTLAKIFCLSVVIEAINLVMPVGTQLVMDHAIPAGDRGLLTLISAGLMF
                     FILLRAATGMLRAWSSLVMGTLINVQWQSGLFNHLLRLPLAYFERRKLGDIQSRFGSLD
                     TLRATFTTSVVGAIMDSIMVVGVFVMMLLYGGYLTWIVLGFTTVYVLIRLVTYGYYRQI
                     SEETLVRGARASSYFMETLYGIATVKIQGMAERRGTHWLNLKIDAINSGIKLTKMDLFF
                     GGINTFVAACDQVAILWLGTSLVIDNQMTIGMFVAFGSFRGQFSDRVASLTNFLLQLRM
                     MSLHNERIADIALHEKEEKKPELDIVADMTPVSLETTDLSYRYDSQSAPVFSGLYLSVT
                     PGESVAITGTSGAGKTTLMKVLCGLFEPDSGKVLVNGTDIRQLGINNYHRMIACVMQDD
                     RLFSGSIRENICGFSEEMDEAWMIECARASYIHDVIMKMPMGYETLIGELGEGLSGGQK
                     QRIFIARALYRRPGILFMDEATSSLDTESERFVNVAIRNMNITRVIIAHRETTLRTVDR
                     TISI"
     CDS             11793..12203
                     /codon_start=1
                     /note="putative fusion protein; N-terminus similar to McmI,
                     microcin M immunity protein; C-terminus similar to McmA,
                     microcin M precursor"
                     /product="MceK"
                     /protein_id="ACF93948.1"
                     /transl_table=11
                     /translation="MKEGGYSKKVDIKTTIIAFVINYLFFYITVSLYLSYYYGYDFFNL
                     YMLFLSLAITSLSLWLNVRFYFFYRFNNKGVKMRKLSENEIKQVSGGDGNDGQAELIAI
                     GAIAGTFLSPGIGSIAGAYVQWSLALEWRSAL"
     gene            complement(12400..13092)
                     /gene="mceF"
     CDS             complement(12400..13092)
                     /codon_start=1
                     /gene="mceF"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /note="similar to McmM of CAAX amino terminal protease
                     family"
                     /product="MceF"
                     /protein_id="AAL08399.2"
                     /sec_met_domain="Abi (E-value: 6.8e-14, bitscore: 42.3,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="METNKYNGEYIKKNRNSTGCFSMFVLFFAITFTPLFFKNSPEIMS
                     RGLLAPMLFILEFIILVPLYYLFFRKRDGLGIGTFNLKLFFILFFIIIFIQFMVPYLLG
                     FRKAESWSTSQISYEHYIFWINVLPAIFIVPIYEEIVFRGCLFNSFKFWFNGNVYGSAV
                     VTSFLFAVMHLQYSDIRTFVMLFLVSLTLIVARVKSNGILMPILLHMLMNVVVIGVQYL
                     VYINHAHQ"
ORIGIN
        1 gatccttaat ggcatcatcc agtagctggc tcttgggaat tcacgtcttt tgtgctaact
       61 gtactacgtc aaactaaccg tcattcatct tcacacatag acatacaggt aatcagaaat
      121 tttcaggcag tattctggtg cagttgcaca tacgtaacta caggctttga cctttaggca
      181 taaggatctc gcaccacccc ggaggggtgg tgcggggaac ggatgttacc gtaaaagagt
      241 tgcgttaatt catgttaact accactaccg gaactggatg ttgcgctgtt ataaccacta
      301 ccgctaccat tccagcttgg cccgatgagt acagggatgg ggacatttac aggaccatgg
      361 tcaattaatc cttgccctac agtttgtaat gcacctcccg cggcccccaa agctgctgat
      421 cctaatccgc caggagcgcc aagagcagca ccccatgcca tattatttcc aaggtcgttt
      481 agaagttgag tatttggatc ggtctctcct gcaccaaaag caagatttaa gtccttttga
      541 ctaatttctc tcattcttag ctccatttat tgtaagtatc actacgttaa ttgctattgc
      601 aattaacgta gaaataaagc aaaagaggta gaatttgtca aatataccgt aacttcccac
      661 cagatacact tttgttaatg gatataaaaa acaaataaaa aaagttagta aaatcagaac
      721 aataactttg tttcgcgttc tctgcggata gaatttacta cgtgaaatga tacagaacgc
      781 catgactgaa aagaaaacag gagaaaatcc aaatgaaagt aatgtcataa gttttatccg
      841 ttaactgaag cgcattgatt cattttatta ttaaggaatt ctaattagac gtcaactacc
      901 ttaattgggc atcaagtcgg cgcttgatac gcatgtgttt atgctgcagg gagcaagtaa
      961 catgcttctc tcaaagagtt tagtctacaa aattcatggg gaggttcatg acttaccttt
     1021 gagcaccttt tctatcagtc ttttatgtaa cctgtgtcag gcataattat tcaccagaat
     1081 taaggatatt gacagtaagg ttttgcagga gcatcttata gatgagaatc agttagttgc
     1141 atatatacta tggccttcaa ctgaacttcc tgtggaggcg aagtaaggat tttgttcaga
     1201 ggttggcgtc ttaaagccac gacgaagtaa tcctctcgtt gtttccagag tgatgtgttt
     1261 ttctgatgag tggattaagt tcctaaaacg gagaaggatt ttcttgtgcg tattctcttt
     1321 attggccctc ctttgtatgg gctgttatac ccggtaattt ctctggctca ggcttttcgt
     1381 gtgaacggac atgaagtagt ggttgccagt gcaggtaaat tcacgcataa agcggcagat
     1441 gctggtctgg ttgtttttga tgccgcacca ggactggatt cagaagcggg ttatcgccat
     1501 caggaggagc tgagaaagaa aagtaacact gtggggcatt tctctttttt cagtgatgag
     1561 atggctgacc gtctcgttga ttttgcagga caatggcatc cggatttaat cgtttacccc
     1621 ccacttggtt tagccggacc gctgattgcc gctaaatatg ggatcccatc ggtcatgcag
     1681 acggttggat ttgcccatac ttctgcccac atccagatgg taactcgttc tctgggaaat
     1741 gcttacaggc gtcatggtgt cagtggcccg ccgcgtgatc ttgcatggat tgatgtcaca
     1801 cctccgagta tgagcattct taaaaatgat ggtgagcctg ttatttccat gaggtatgtg
     1861 ccctacaatg gaggtgctgt ccgggagccg tggtgggaca gagcaccggg aagaaaacgt
     1921 ctgttgatca gccttggtac agtcaaacca atggtggacg ggctggattt gatatcatgg
     1981 gtgatggatt ctgcaaatga ggtggaagct gacattattc tgcaactggc gatgaatgcc
     2041 cgtgcaggat tgcgaaaatt gccatcaaat gtgcgtctgg tagagtggat ccctatgggg
     2101 gtatttctca acggcgctga tggatttatt catcatggcg gtgcgggtaa taccctgaca
     2161 gccctgtata acgggatacc gcagattgtg tttggtgaag gtgctgatcg ccctgtgaat
     2221 gcaaaagcag ttgctgagcg tggatgtggg atcattcctg gcgaccatgg cctgaccagt
     2281 gatttggtga atacctttct ttatgatgat tcacttcgct tatgttcagc acaggttgca
     2341 gctgaaatgg ctgaacaacc cagccctgct gagatcgctg gtgttctggt agcgaaactg
     2401 aaaaccatct ggcaataatt gtataacact gctgaaatta ttcacgaaag ctgccaaagt
     2461 aaatagaaag tccttcatca tatgagtgaa ggacattact gagtgggaaa tgaacatgaa
     2521 ataatgtagc agaaagggtt accagaacgg tgaaattatt gccgacaggg cattactctc
     2581 tggtatctgc tcacgattac acatctgata atccatcgat aattgctcca cgccaccagg
     2641 cataatcatg acctccggta tagacagaaa gtttactttt caaaccactg gcgagcaacg
     2701 attggtgtaa ctgctgaaca tgagggactg tagcaccttc cagagaaccg acccccatct
     2761 gaatatttac atctttcgga agcgcagaaa gtacctgttt acttacccag gaggtatcat
     2821 tctccgtaaa catgagagga gtgtttctgt cgggactcca ccacattgaa ggggagtggc
     2881 tgattactgt gccaaatata tccggtgcat ataaggccgc catcagcgcg gtgacaccgc
     2941 caaggctttg tccggccagt atggtattcg agcgacctgc ccatacgata tcaggataat
     3001 ccttatacaa tcgggggatt aaatttcctg cgatatcgag aatgagttct ttgttacccc
     3061 ccagaatatt cacacgatca gattcattaa tattgtctat tccaaggatc gccatcggcg
     3121 caatacgtcc agtagtaatg gctatgtcta ttgcccgggt aatacctata cggtcaaacc
     3181 atgtttctgc atcaggtaat actagtaatc ccagaggaac tgacgttgga taccccggga
     3241 aatataaacg aatctggcgt tggtgtcccg caacaaaaga atatgatgtt gaaaggacgc
     3301 cggaacaggt gtgggaagta tcatcccact ccgtttgtcc aggagccaga tcaagagaaa
     3361 ggactgattc cccgaatcct cgtatattta tttctgatgt tttgttaaac gggtcaggtt
     3421 ggccgggtaa cggtgagaag cgacctccaa gctgagaaat atcttcttgc ggcatttccg
     3481 gtggaatttc tataaacgag tatgaaccac gatatgaagc aggtaactcc agagtcagtg
     3541 tccagatatt cgtcgaaggg atatgagtca taagtccttt tttgacatct cttctatctg
     3601 taacacggtt caggcgaaga tatacgcctt gtagcacttc ttctgaacgc cacagaaatg
     3661 tgacctcccg attttctgtg ttgctgctgc tttttgtcat caggggaaga gcagcagatt
     3721 caacttcgcg ccagaaatga gtgatatcca tggatggatt tgtggacaac tgtgcacata
     3781 ttcttgattc cgtacagata tcatcaagac ttctggatgg atgattcctc ataaatggca
     3841 taccttgtag acaaaaatta tatattataa ggatgatgag aagctctctt agctatgaaa
     3901 gaggattcac ttcccgttta cataccgctg gttggcgtac ctgggcccct gcaggcacca
     3961 tttgcgtcaa tatagcttcc tgtgcccgtg ccgcgttgat ttgttatatt agcagagccg
     4021 cgtagcccat caaaatttaa ccattcctta acaaaattaa gcctgctacc catgatttct
     4081 ctcatcaatt aatctcctga ttattgtcac ttacctgttt attttaaatc acattcactt
     4141 gttatagcag aatctaactc attattgaaa ttgctttttg ttaacacatc tcatattttt
     4201 atatttatta aaaagaaagt caatattttc taacctctgg taacaaagga aatacacgga
     4261 ataaagttta gcctcattcg gtaatgcact attttcatta tgcccataaa tatattattg
     4321 ggattgtcgg acagaagaat gtggttgtgt ttttttaccc tccttctgtt gtcctacaag
     4381 agtattcata ccgataggaa aataaggttc cttttcaggg ccttgtttaa tggttttaat
     4441 ttctgatgaa tgagcactca atgttagaat tactataaaa gttaccataa tctttttggc
     4501 aacgagtata ttggttatca cttcctgaac tgcgagggaa gttcagatat gtaaacagtt
     4561 ttctttatta taaattttaa gtttggttgc tctgaataca gactggccac aatagatcgt
     4621 aaatgaattt attgtggcag atatatattg tcaccttttg ttattgtaac agaggaaaca
     4681 ttttaataag caaaatgcat tttgataaca gggagcataa caacaaaaga aacagttatc
     4741 agcatcgcta aaaaaaactc tgtatttgtt ccatgacgaa acacctgata gatgaatgaa
     4801 ataccagcaa ggatagtgct ggaataaata caaagaaatt ttggcgagtc taaagcggaa
     4861 ctgccaggaa gtgcaaatga agtaagaaga ccaataccta atataaataa gggtattttg
     4921 tctaaataga gaaaaatcat agcaatatct cccaaaaatg tttaacttcc gctggaagaa
     4981 gaatttgatg gtgaactaaa ggaatcatag ataaaagcac caccatagcg acctgccgca
     5041 gcacctgcaa caaacccgac aacagttccg acaggtccgg ccaaactccc aactgtagcc
     5101 cccatttgtg ctccggccca tccccctgcg gctgcacctg ctgtacctcc tgctaccgct
     5161 ccagccactg ctgcatcacc tcgatctgcg ccggatactg caaaaaaatc attttctgtc
     5221 agtgctctca ttcatcatct cctcaccgtt aagatgtcca gttttgacat tatccccaca
     5281 gtttgctcag aatgagcagg ttaacatctt caatccttaa gtgacctctg ggtcgatacc
     5341 agtgtatggc agaaagatac tgcttttttg ttataggtca tgaccgcagc cgcagcaact
     5401 ttcacaaccg catccacaaa tataattatc atccccatac tctcgtttat cctgggctga
     5461 atctatctgc ataatatgct ggttgcattc tgtatccgca atatcctgat attcagaaac
     5521 agtatttcct gtccggatgg aataattagc ttttaatgct gtgttttctg gggatggcag
     5581 gggctggggg gaatagagga gagtgaggaa atcaccatcc tctttactca cctctgttac
     5641 ctgctgatgt tggacttcat ttacattatt aatgcttatg ttttttgttt ttttgtgagc
     5701 tttgttgcca ttcatttcag tctcccatca gttaactacc attaatagta gttaacataa
     5761 tcagtacgta accaattctt aggccagggc ggccccaacc aaaccccgta tgctgagtga
     5821 gcacattggt atatatacat caaaaaaaat taacatttga cttcgtgtgt ctaatatgtc
     5881 agctttgtaa gtaaaagtaa ataacaatca ttctcttctt tgtgaggtaa gtgaaatggt
     5941 gtttgctaca gacctggtgg cggcagatga cgggttaaga acaacgttgc tggctttgat
     6001 catgaccgcc acattgctcg agtattcttt gtcaatgttc tgagcttttg taaccagaaa
     6061 gaaatcttat gcgatcttat tccgtatagg atcccttgtt tttattggtg ttgggttaca
     6121 caactgattt gggtatgcaa aaaaggatga agcctttccg gagtcagcga ctcctcaggc
     6181 catggtaacg gagaaataca gtcaaatgaa ccagctatgt tcgcttgctg aactgaatga
     6241 caacctggtg cccttcactg cccgacggat aacctcttcg ttgatttggt gtgctgaaaa
     6301 tgtccgagat acagaagtat tactaaaagc atgcagctat ataatagatc cggtcagttc
     6361 agcttctgcc aaaacattcc acgcagagcg ctatggtgga agtggtattc agcgtaatgg
     6421 cggtggcgca cgttgtggat ttgatggtaa ctatcaggtt aaaggcatgg gagccaatcc
     6481 tttagtcggt gtagggactg acggacgtca ttctaacggc gcactcggtg ccattcatgc
     6541 aatctatgag gctttatggg gagaggtact ggcgcaaata ttaccctata gtgctgtgcg
     6601 ggttcaggca gttttactta ccaatctcta taccgacaaa gcatttgatc gctcccatgg
     6661 gaaatcccga cgagccctgt tggtgcgcga ccccgtggtt cgcccggcgc attttgaacg
     6721 cgcgccttac ttccgggcta agccggaata tgctggtcag ttaattcatg atgcccgtcg
     6781 ggtcaggtcc gtgatccgca tgctgccaag taatttacct gtaccatcag aagggtttag
     6841 tggagaagcc cgacgtaatc cccggattta ttgtattgag gggttatgtg aactggcccg
     6901 ccgtgaagca tggcagatgg cattttgtcg tacacgtttc ctgagattga cgacttctcc
     6961 ttctaatatt gcgatggatg gccggttaat ggattttaac gggctcagct gtttgttccc
     7021 gggagattac cctgacaatt ttggacacca gttcagactt gccgaactgg tgaaagaacc
     7081 gatggtactg atacaggggc tgtccgatct ctgtttgtac atcggaaaat acatgtttga
     7141 ccctgatttc acactggtgt ctcgtctgaa ggttgaggag acctttcaga aaactttcca
     7201 tgaagcatgc tattactgtt atctggaaca gctgggtatc cccacagagt ttctaccaca
     7261 ggaagggata ccggatacgt tgaaacaact ggtcaacagc ctcgttgtac ttgtcaataa
     7321 acgcagcgac cgattgtgct gtccagatac agcctgtgga gatgattcac cactgcaaag
     7381 cctggtggtg gaactgattc ggcagagcca ggcccaaacc cacccattgg ataatgacgc
     7441 tgagcatgat gttcatttta ccgaagcaca actgtgtttt tcccgggcta tccactggct
     7501 gacgcaggac agtatcagac gtcagatcaa tgtttcatcc ctcctgaaag aaatggaaaa
     7561 ccatgccagg aagaggctgc aacccagaaa atggctggga aaggcgtgtc tgtctgagga
     7621 gattgcctct cttctggatg aacacagcga taacccctat tacctgcggg aggcattttc
     7681 tgatatggga acccgaatgc aggcattttc cagagaagcc ttcggccatg taaaccctgt
     7741 cagaattgcg gtgtaaaata taaggataat gacaatgatg tcgcatatac gagaatccat
     7801 caggggcaga gaggaatgga ccgtctatga gcagattggt tttgcagtca gttgcatgct
     7861 tcataaccgt aactacagtc tgtatccggt gttaaccatt caatactgga ctgaatatgc
     7921 gatacagcac aatcagataa agttcctgtt tgattcgcgc ggttttccac tggcgtatat
     7981 tacctgggcg tatcttgagg cagatacgga agcacgcctg ctcaacgatc cggagttcag
     8041 actacatcct tctgaatggg atgaagatgg aaggatctgg atcctggatt tctgttgtaa
     8101 gcccggcttt ggccgaaagg ccattgagcg tttatttcag cttcgcccgt ggggggaggg
     8161 agaagtgcgc tggctgagca ggaggaagaa aatcatgaca tttcggccca tgcggcgaca
     8221 taaaatgccg atggccccgg agacacagaa agaactttga ataccggagg cgaaatgtac
     8281 cccggtatca ttttgatgga acgctgcatt ttcggagttt tgaatatacc tgttcggatg
     8341 agttctgaag gtatgacagg aaacgtgtga gtaaggacat atattttgtt tcgtcaggat
     8401 gcgttagaaa acagaagaat gaaatggcgg ggacgggcaa ttttacttcc cgggataccg
     8461 ctctggttaa ccatgctggg aagcattgcg tttatcacgg catttctgac gttcgttata
     8521 gctggcacct atagccgtcg tgtcaatgtc agtggtgagg tcacgacctg gccgcgagcc
     8581 gtcaatatat actccggtgt gcaggggttt gtagtcaggc agtttgtaca tgaaggtcag
     8641 tcgataaaaa aaggtgatcc gatttaccag attgacgtca gtaaaagcac acgcagtggc
     8701 gttgtcaccg ataatcagcg cagggatatc gaaaaccagc tggttcgagt ggacaacatc
     8761 atttcccgtc tggaagaaag taaaaaaatt acgctgaata cgctggaaaa gcaacatctg
     8821 caatacagcg atgcgttccg tcgctcatca gatattatac ggcgggcaga ggaagggata
     8881 aagataatga agaacaacat ggagaattac agaaactacc agttaaaagg gctgattaat
     8941 aaagatcagt taactaacca ggtggcatta tattatcagc aacaaaataa tcttctcagc
     9001 ctgagcggac aaaatgaaca gaatgccctg cagataacca gtctggagag ccagatccag
     9061 actcaggctg cggattttga taaccgtatc tatcagatgg aactgcaacg gtacgagttg
     9121 caaaaagagc tgattgatac tgatgtgggg ggggaaatca ttatccgtgc tttgtctgac
     9181 gggaaagttg actctctgag tgtcactgtc gggcaaatgg tcaatgccgg agacagtctc
     9241 ctgcaggtaa tccctgaaaa tatcgaaaac tactacctta ttctctgggt ccctaatgat
     9301 gctgttcctt atatttcggc aggagataaa gtgaacattc gttatgaagc ctttccggca
     9361 gaaaaatttg gtcagttctc tgctacggtg aaaaccatat ccagaacgcc agcctcaacg
     9421 caggaaatgc tgacctacaa gggggcacct caaaatacgc tgggcacttc tgttccctgg
     9481 tataaggtca tcgttaaacc tgaaaaacag attatttctt atggcgggaa atctctccct
     9541 cttgagaatg ggatgaaagc tgaaagcaca ctattcctag aaaaaaggcg tatttaccag
     9601 tggatgcttt ctcctttcta tgacatgaaa cacagtgcaa cagggccgat cgatgagtaa
     9661 cgggaatgtc agacgaatga taaatcagct tgatatgcgc tggcgacgtc gggttccggt
     9721 tattcatcag acggagacct cggagtgtgg actggcctgt ctggcaatga tatgtggaca
     9781 ttttggcaag aatattgacc tgatatccct tcggaggaag tttaatcttt ctgcccgtgg
     9841 ggcaaacctt gcgggaataa atagtatagc ggaacagctg gggatggtaa cccgggcact
     9901 ttcgctggag ctggatgaac ttggtgcgct caaaacgcca tgtattctcc actgggattt
     9961 cagccatttt gttgtactgg tcagcgtaaa gcgtaaccgt tatgtactgc atgatccggc
    10021 cagaggcaga agaaatgtcg gactggagga aatgacccgg tattttaccg gagttgcgct
    10081 tgaggtctgg ccaggcagtg aattttcgga ggagaccacg cagaatcgaa ttcaactacg
    10141 ttctcttatt aacagcattt acggtattaa aagtacactg gcgaaaatct tctgtctgtc
    10201 ggttgtgatt gaagctatca atctcgtaat gcctgtgggt actcaactgg ttatggatca
    10261 tgcgattccg gcgggtgaca gagggctgct tacactcatt tctgctggcc ttatgttctt
    10321 tatactgcta agggcagcta cgggtatgct ccgtgcatgg tcctcgctgg tcatgggcac
    10381 gctcatcaat gttcagtggc agtcgggtct gtttaaccat ctgctcaggc taccgctggc
    10441 atattttgaa cgtcgcaaat taggtgatat ccagtcgcgt tttggctccc ttgataccct
    10501 gagagccacc ttcaccacca gtgtggtggg ggccattatg gacagtatta tggtggtggg
    10561 tgttttcgtg atgatgctgt tatacggagg gtatcttaca tggatagttc tcgggtttac
    10621 cacggtttac gttctgattc gtctggtgac atacggctat taccggcaga tatcggagga
    10681 aacgctggtc aggggagcca gagccagttc ctattttatg gaaaccctgt atggcatcgc
    10741 cactgtaaaa attcagggca tggctgaacg gcggggaacg cactggctta acctgaaaat
    10801 tgatgccatc aattcaggca ttaagttaac caaaatggat ttgttctttg gcgggataaa
    10861 tacttttgtt gcagcctgtg accaggtggc tattttatgg ctgggaacca gccttgtgat
    10921 cgataatcag atgacaatag ggatgtttgt ggcgtttggt tctttccgtg ggcagttttc
    10981 ggatcgggtt gcctctctga ccaactttct tttgcaactg agaatgatga gtctgcataa
    11041 tgagcgtatt gctgatatcg cactgcatga aaaggaagag aaaaaacctg aattagatat
    11101 tgttgctgat atgaccccgg tctcactgga aaccactgat ttaagttacc ggtatgacag
    11161 ccagtcagcg ccggtgttca gtggtctgta cctgtcagtg actcccggag agagtgtggc
    11221 cataaccggt acatccggtg ccggaaaaac cacgttaatg aaagtattat gtgggttgtt
    11281 tgaacctgat agcggaaaag tactggtgaa tggtacggat atccggcagc tggggataaa
    11341 taattatcac cggatgattg cctgcgtgat gcaggatgac cggttgtttt cagggtccat
    11401 cagagagaat atttgtgggt tctctgaaga aatggacgag gcctggatga tcgagtgtgc
    11461 cagggccagt tatattcatg atgtgataat gaaaatgccg atgggatatg aaaccctgat
    11521 tggtgagctg ggtgaagggc tttccggagg gcaaaagcaa cgtattttta ttgccagagc
    11581 tctgtaccgt agacctggaa tattatttat ggatgaggct accagttcac tcgatacaga
    11641 aagtgaacgc tttgtgaatg tagcgataag gaatatgaat atcacgagag tgattatcgc
    11701 acacagggag acaacgctga ggactgtcga ccgaactatt tcgatttaaa cttcatttgt
    11761 ttggactgat attatcttgt aaggaggttt tagtgaagga aggaggatat agtaaaaagg
    11821 tggatataaa aacaaccata attgcttttg ttatcaatta tctgtttttt tatataacag
    11881 tgtcattata tcttagttat tattatggat atgatttttt caatttgtat atgttgtttt
    11941 tatctcttgc aattacgtca ttatcactgt ggttaaatgt aagattttat tttttttaca
    12001 gatttaataa caaaggtgtt aaaatgagaa aattatctga aaacgagatt aagcaagtat
    12061 ccggaggaga tgggaatgat ggtcaggcag agttaatcgc tatcggtgca attgcgggta
    12121 cgttccttag tcccggaatt ggatctatag ctggagctta tgtccaatgg agcctagcat
    12181 tggagtggcg gtcggctctg tagttggtgc attttgttat ggaattggaa atagttctct
    12241 attcagttcc tcttcagttg ccgggagcag gatcttcaag gttttctgtg ggtaaatatt
    12301 aattcttgac atttttcttt ggaggggaga ttattttggt gagtaatcca ctctggatat
    12361 tgttttttct tcagcatcca gagcgaagca tattatggac tattgatgag cgtgattgat
    12421 atatactaag tattgcactc caataacaac aacattcata agcatgtgaa gcaatatagg
    12481 cattaatata ccatttgact taactctcgc cacaatgagt gttaatgaaa ctaaaaatag
    12541 cataacaaaa gttcgtatgt cagaatactg aaggtgcatt acagcaaata gaaatgaagt
    12601 aacaacagct gaaccataaa cattcccgtt gaaccagaat ttaaaggaat taaaaagaca
    12661 acctctgaac acaatttctt catatatggg cactataaaa atagcaggaa gaacattaat
    12721 ccagaaaata taatgctcat aagatatttg ggatgtgctc catgattcag ccttcctgaa
    12781 ccccaacaaa tatggcacca taaattgaat gaatataatt ataaaaaaca atataaaaaa
    12841 tagcttcaag ttgaaagtgc caatgccaag cccatctctt ttcctaaaaa acaaataata
    12901 caacggaact aaaataataa attcgaggat gaataacatg ggggcaagaa gtcctcgaga
    12961 cataatttcc gggctatttt tgaaaaatag tggagtgaag gtaatagcaa aaaacaatac
    13021 aaacatagaa aaacagccgg tgctatttct gttctttttt atatactcac cgttatactt
    13081 gttggtttcc attttgcctc cctgttgctt gtcatggcaa taattttaaa ttaaaggatt
    13141 tggagtggat tatttcgttt ccataacgaa aatcgaacga tgtaactacg tgattctggg
    13201 gatgacatat acattgatag ttccttctga aattgtcctg aattcataat ttaagctgct
    13261 attcaaacca gcaatattta aggaagttat aagagcactg atatcgaaac tgaaggcgac
    13321 attctccaaa atgcctcttt tatttttctg tacatgtggc attcaaggta tatgttcgtt
    13381 agatcggctc ttatcaagag ctctattata aggggggtat gaatgattat caatcgtgtt
    13441 ttataaaaat tatcattcaa gctattgtca aatatagtat ggctttactt acgcacacca
    13501 cttagtaaac atgtatatgt atgacggttt agttgcataa aaattgacta cagcgccctc
    13561 ttctatattg gaatgatggc taatcatctt ttcgactcct ccgaaagtgg ttcgaactca
    13621 gccaatcatt tcaggaatta acgattatag ggctgactac atttttgact tctttactac
    13681 agagtgttta tggttgaatg aaagagagct agggttacgc ttatcattgg atatagtact
    13741 t
//
