LOCUS       BGC0000589              8421 bp    DNA     linear   BCT 14-NOV-2006
DEFINITION  Escherichia coli Genomic Island I, strain Nissle 1917.
ACCESSION   BGC0000589
VERSION     BGC0000589
KEYWORDS    .
SOURCE      Escherichia coli Nissle 1917
  ORGANISM  Escherichia coli Nissle 1917
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
COMMENT     ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2024-10-17 13:53:24
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 37232
            Orig. end    :: 45653
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     subregion       1..8421
                     /aStool="mibig"
                     /contig_edge="False"
                     /label="ribosomal"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..8421
                     /contig_edge="False"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            1..120
                     /gene="mchX"
     CDS             1..120
                     /codon_start=1
                     /db_xref="UniProtKB/TrEMBL:Q83ZA9"
                     /function="required for microcin H47 production"
                     /gene="mchX"
                     /note="ORF38; possibly involved in a regulatory loop
                     modulating its own expression and that of mchA and mchB"
                     /product="protein MchX"
                     /protein_id="CAE55697.1"
                     /transl_table=11
                     /translation="MEFATNRVSVNDSRSALSSTLLLSLIMSATLLEYSLSMT"
     gene            189..398
                     /gene="mchI"
     CDS             189..398
                     /codon_start=1
                     /db_xref="UniProtKB/TrEMBL:Q83ZA8"
                     /function="protects microcin H47 producing cell against
                     microcin H47"
                     /gene="mchI"
                     /note="ORF39"
                     /product="microcin H47 immunity protein MchI"
                     /protein_id="CAE55698.1"
                     /transl_table=11
                     /translation="MSYKKLSQLTAIFSLPITILLVSLSSLRIVGEGNSYVDVFLSFII
                     FLGFIELIHGIRRILVWSGWKNGS"
     gene            415..642
                     /gene="mchB"
     CDS             415..642
                     /codon_start=1
                     /db_xref="InterPro:IPR002453"
                     /db_xref="UniProtKB/TrEMBL:Q7ATN3"
                     /function="bactericidal antibiotic"
                     /gene="mchB"
                     /note="ORF40"
                     /product="microcin H47 precursor"
                     /protein_id="CAE55699.1"
                     /transl_table=11
                     /translation="MREITESQLRYISGAGGAPATSANAAGAAAIVGALAGIPGGPLGV
                     VVGAVSAGLTTAIGSTVGSGSASSSAGGGS"
     gene            914..2464
                     /gene="mchC"
     CDS             914..2464
                     /codon_start=1
                     /db_xref="InterPro:IPR006089"
                     /db_xref="UniProtKB/TrEMBL:Q6KDB6"
                     /function="putative microcin production-related"
                     /gene="mchC"
                     /note="ORF41"
                     /product="MchC protein"
                     /protein_id="CAE55700.1"
                     /transl_table=11
                     /translation="MSHQCSLSELNENLVPFTARQIKSSLIWCAEDVRNPDELQNACSY
                     IIDPGSTASAKVFHAERYGGSGIQRNGGGARCGFDGNYQVKGIGSNPLVGEGTDERHSN
                     GALGAVHAIYEALWGEVLAQILPYSAVRVRAVLLTDLYTEKAFERSGRKSRRALLVREP
                     VVRPAHFERAPYFQVKPEYSSLLIHDACRVRSVIHKLPGYLPVPPEEIDAEARTDPRIY
                     CIEGLCELARREAWQMAFCRTRFLRLTTSPSNIAMDGRLMDFNGLSCLFPGDSPADFGY
                     KLRLAELAKEPMVLMQGLSDLCLYIGKYMFDPDFTLAARLKVEEIFQKTFHEACYYCYL
                     ELLGIPGEFITQKEIPDILKELVNSFVALLNKYYEKSHAQDIVNQDGSPLQKLVVTLIH
                     HRHNQKQALNSSIKNDVYFTVAQQCFSQAIHWLTQGSTRRQINASLLLKEIEHHTMKRL
                     QPREELRKENMCEKIAILLDNHGDDPLFLQEAISDMKNFMLKFSRDAFGYLEPIRNTV"
     gene            2490..2942
                     /gene="mchD"
     CDS             2490..2942
                     /codon_start=1
                     /db_xref="GOA:Q83ZA6"
                     /db_xref="UniProtKB/TrEMBL:Q83ZA6"
                     /function="putative microcin production-related"
                     /gene="mchD"
                     /note="ORF42"
                     /product="MchD protein"
                     /protein_id="CAE55701.1"
                     /transl_table=11
                     /translation="MSYIRETIRGKDEWTVYEQIGFAVSCMLYNRNYSLYPVLTIQYWT
                     EYAIQHNQIKFLFDSRGFPLAYITWAYLEADTEARLLRDPEFRLHPSEWNEDGRIWILD
                     FCCKPGFGRKVIDYLIQLQPWGEGEVRWLSRRKKIVTYIPERLHKT"
     gene            3128..4369
                     /gene="mchE"
     CDS             3128..4369
                     /codon_start=1
                     /db_xref="GOA:Q6KDB4"
                     /db_xref="InterPro:IPR003997"
                     /db_xref="InterPro:IPR006143"
                     /db_xref="InterPro:IPR006144"
                     /db_xref="InterPro:IPR011053"
                     /db_xref="InterPro:IPR011055"
                     /db_xref="UniProtKB/TrEMBL:Q6KDB4"
                     /function="microcin secretion-related"
                     /gene="mchE"
                     /gene_functions="transport (smcogs) SMCOG1029:RND family
                     efflux transporter MFP subunit (Score: 72.3; E-value:
                     5.9e-22)"
                     /gene_kind="transport"
                     /note="ORF43"
                     /product="microcin H47 secretion protein mchE"
                     /protein_id="CAE55702.1"
                     /transl_table=11
                     /translation="MKWQGRAILLPGIPLWLIMLGSIVFITAFLMFIIVGTYSRRVNVS
                     GEVTTWPRAVNIYSGVQGFVVRQFVHEGQLIKKGDPVYLIDISKSTRSGIVTDNHRRDI
                     ENQLVRVDKIISRLEESKKITLDTLEKQRLQYTDAFRRSSDIIQRAEEGIKIMKNNMEN
                     YRNYQTKGLINKDQLTNQVALYYQQQNNLLSLSGQNEQNALQITTLESQIQTQAADFDN
                     RIYQMELQRYELQKELVNTDVEGEIIIRALTDGKVDSLSVTVGQMVNPGDNLLQVIPEN
                     IENYYLILWVPNDAVPYISAGDKVNIRYEAFPAEKFGQFSATVKTISRTPASTQEMLTY
                     KGAPQNTPGASVPWYKVIAMPEKQIIRYDEKNLPLENGMKAESTLFLEKRRIYQWMLSP
                     FYDMKHSATGPLND"
     gene            4362..6458
                     /gene="mchF"
     CDS             4362..6458
                     /codon_start=1
                     /db_xref="GOA:Q6KDB3"
                     /db_xref="InterPro:IPR001140"
                     /db_xref="InterPro:IPR003439"
                     /db_xref="InterPro:IPR003593"
                     /db_xref="InterPro:IPR005074"
                     /db_xref="InterPro:IPR011527"
                     /db_xref="UniProtKB/TrEMBL:Q6KDB3"
                     /function="putative microcin H47 secretion ATP-binding
                     protein"
                     /gene="mchF"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288:ABC
                     transporter related protein (Score: 251.5; E-value:
                     2.9e-76)"
                     /gene_kind="transport"
                     /note="ORF44"
                     /product="probable microcin H47 secretion ATP-binding
                     protein mchF"
                     /protein_id="CAE55703.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 1.9e-42, bitscore:
                     133.8, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTNGNFRQIINQLDMRWRRRVPVIHQTETAECGLACLAMICGHFG
                     KNIDLISLRRKFNLSARGANLAGINGIAEQLGMITRALSLELDELGALKMPCILHWDFS
                     HFVVLVSVKRNRYVLHDPARGRRYLGREEMSRYFTGIALEVWPGGEFRTETQQTRISLR
                     SLINSIYGIKRTLAKIFCLSAVIEAINLVMPVGTQLVMDHAIPAGDRGLLTLISAGLMF
                     FILLRAAVSMLRAWSSLVMSTLINIQWQSGLFNHLLRLPLAFFERRKLGDIQSRFGSLD
                     TLRATFTTCVVGAIMDSIMVVGVFVMMLLYGGYLTWIVLGFTMVYVLIRLVTYGYYRQI
                     SEETLVRGARASSYFMESLYGIATVKIQGMVGIRGTHWLNLKIDAINSGIKLTRMDLLF
                     GGINTFVAACDQVAILWLGASLVIDNQMTIGMFVAFGSFRGQFSDRVASLTSFLLQLRI
                     MSLHNERIADIALHEKEEKKPEIEIVADMSPVSLETTDLSYRYDSQSAQVFSGLNLSVA
                     PGESVAITGASGAGKTTLMKVLCGLFEPDSGKVLVNGTDIRQLGINNYHRMIACVMQDD
                     RLFSGSIRENICGFAEETDDEWMTECARASHIHDVIMKMPMGYETLIGELGEGLSGGQK
                     QRIFIARALYRKPGILFMDEATSSLDTESERFVNAAIKKMNITRVIIAHRETTLRTVDR
                     IISI"
     gene            6494..6715
                     /gene="mcmI"
     CDS             6494..6715
                     /codon_start=1
                     /db_xref="UniProtKB/TrEMBL:Q83TS3"
                     /function="putative microcin production-related"
                     /gene="mcmI"
                     /note="ORF45"
                     /product="hypothetical protein McmI"
                     /protein_id="CAE55704.1"
                     /transl_table=11
                     /translation="MGEVKKDIKITVIAFVINYLFFYIPVSLYLSYYYGYNFFNLYMFF
                     LSLVVTFLSLWLNVNFYFFTNLIAKVLK"
     gene            6712..6990
                     /gene="mcmA"
     CDS             6712..6990
                     /codon_start=1
                     /db_xref="UniProtKB/TrEMBL:Q83TS1"
                     /function="microcin production-related"
                     /gene="mcmA"
                     /note="ORF46"
                     /product="McmA protein"
                     /protein_id="CAE55705.1"
                     /transl_table=11
                     /translation="MRKLSENEIKQISGGDGNDGQAELIAIGSLAGTFISPGFGSIAGA
                     YIGDKVHSWATTATVSPSMSPSGIGLSSQFGSGRGTSSASSSAGSGS"
     gene            complement(7165..7851)
                     /gene="mcmM"
     CDS             complement(7165..7851)
                     /codon_start=1
                     /db_xref="InterPro:IPR003675"
                     /db_xref="UniProtKB/TrEMBL:Q6KDB0"
                     /function="putative microcin production-related"
                     /gene="mcmM"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /note="ORF47"
                     /product="McmM protein"
                     /protein_id="CAE55706.1"
                     /sec_met_domain="Abi (E-value: 2.4e-15, bitscore: 46.4,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEMTKNKNATIKKQSSAGCFSMFTLSFSITFIPAFLTNRQELISR
                     GLLVPALFLAEFLILVPLYFIFFRKREGLGRGSFRLDVFFTLFSLIMIIQLFVPYLLAV
                     RKTEEWMISQMSFSGAILWINIFSSVLLVPVYEEIVFRGCLFNSFKFWFNDNIYTSAIV
                     TSVIFSALHLQYTDFHTFLMLFLVSLVLISAKIKSNGLLMPILLHMSMNAVIAGIQYLA
                     SISYTH"
     CDS             complement(7951..8421)
                     /codon_start=1
                     /db_xref="UniProtKB/TrEMBL:Q6KDA9"
                     /note="ORF48"
                     /product="hypothetical protein"
                     /protein_id="CAE55707.1"
                     /transl_table=11
                     /translation="MSCYMKIYWTRKSIPEHSALPPSLRKKNFTDAYNAASSHIEYWIG
                     AGVSFISMMILFRVYDFLLPAQDTFPGDIIRSLCVVCPSILIWFQFSVYVMRKYYRHIL
                     ERGKETETISERLIREADTREYELWRPVHRFFSIVFLIILLVCIHSLITTIK"
ORIGIN
        1 atggaatttg ctacaaacag agtttctgta aatgacagtc ggtcagcact gtcatcaact
       61 ttgctgttat ctttgatcat gagcgccact ctactggaat attctttatc gatgacctga
      121 ctctcctgac tgaggtgcta agatttggta acttttcata atgaaaataa agcaacaggt
      181 aggtattaat gagttataaa aaactgtccc aattgacggc tatattcagt ttacctatta
      241 ctatcttatt ggtttcactt tcatcccttc ggattgttgg cgaagggaat tcttatgttg
      301 acgtttttct aagctttata atatttcttg gttttattga gctgattcat gggattcgaa
      361 ggattctggt ctggtcaggc tggaaaaacg gaagttaaat atgatggagt ttatatgcga
      421 gaaataacag aatcacagtt aagatatatt tccggggcgg gaggtgcgcc agcgacttca
      481 gctaatgctg caggtgctgc agctattgtt ggagctctcg ccggaatacc tggtggtcca
      541 cttggggttg tagttggagc cgtatctgcc ggtttgacaa cagcaattgg ctcgaccgtg
      601 ggaagtggta gtgccagttc ttctgctggt ggcggtagct aattatattt ttatttattt
      661 tacaggtact ttttcggtag tacctgtaaa atatatcctt tttgtttaaa acagatagaa
      721 gatccattta taagtgtgtt tttatgttca acactttact gatgaagata tgatttacag
      781 tcgttgataa ataatttttt aatttatgtg taattaatag agttttattg taataaggaa
      841 gaagctgtca ggacacttac ttgctctttt atgaacactc ttttatgaaa attgaaacgg
      901 agaatactgc cagatgagtc atcagtgttc actttctgaa ctgaatgaaa acctggtgcc
      961 tttcactgcc aggcagatca agtcctcatt aatctggtgt gcagaggatg tcagaaatcc
     1021 agacgagctg caaaatgcct gcagttatat tatcgatcct ggcagtacag cttctgccaa
     1081 agtgttccat gcagagcgct atggtggcag tggtattcag cgtaatggag gtggtgcacg
     1141 ttgtgggttt gatggtaact accaggttaa aggaatagga agtaatccgt tggttggcga
     1201 aggtactgac gaacgtcatt ctaatggtgc actcggcgct gttcatgcaa tatatgaggc
     1261 tttgtgggga gaagtactgg ctcaaatatt accttatagt gctgtgcggg ttcgggcggt
     1321 tttacttaca gatctctata ctgaaaaggc atttgagcgc tccggtagga aatcacgaag
     1381 agccctgttg gtacgtgagc ctgttgttcg cccggcgcat tttgaacggg caccttactt
     1441 ccaagtaaaa ccggagtatt ccagtctgtt aattcacgat gcctgtcggg ttagatctgt
     1501 gatccacaag ctgccaggat atctacctgt accaccggaa gaaattgatg ctgaagcacg
     1561 aactgatccc cggatttatt gcattgaggg gttatgtgaa ctggcacgtc gtgaggcctg
     1621 gcaaatggca ttttgtcgaa cacgtttcct gagattgaca acttctcctt ctaatattgc
     1681 aatggatggc agattaatgg attttaacgg actcagttgt ctgtttccgg gagattcccc
     1741 agctgatttt gggtataaac taagattagc tgaactggca aaagaaccga tggtacttat
     1801 gcaagggctg tctgatctct gcttgtatat cggaaaatat atgtttgacc ctgacttcac
     1861 tcttgcagcc cgtttgaagg ttgaggagat atttcagaaa acttttcatg aagcatgtta
     1921 ttactgttat ctagaactgt tgggtattcc tggagaattt ataacacaaa aagagatacc
     1981 tgatatattg aaagaactgg ttaacagttt tgttgcatta ctcaataaat actacgagaa
     2041 atcacatgcc caagatattg tcaatcagga tggttcacca ttgcaaaagt tggttgtgac
     2101 gctaatccat cataggcata atcaaaagca ggcactgaat agtagcatca agaatgatgt
     2161 ttatttcacc gttgcacaac agtgtttttc ccaggccatc cactggctga cgcaaggcag
     2221 taccagacgt cagataaatg cctcattact cctgaaagaa attgaacatc ataccatgaa
     2281 aaggctgcaa cccagggaag agctgaggaa agagaatatg tgcgaaaaaa ttgccatcct
     2341 gctggataat catggcgatg atcccctttt tttacaagaa gcaatttctg atatgaaaaa
     2401 ttttatgctt aagttttcca gagatgcatt tggatatctt gaaccgataa gaaacacagt
     2461 gtaataaata tataagggaa atagtaatca tgtcttatat aagggaaacc atcagaggaa
     2521 aagatgaatg gactgtttat gaacagatcg gttttgcggt cagttgtatg ctctacaatc
     2581 gtaattacag tctgtatccg gtgttaacca ttcaatactg gactgaatat gcgatacagc
     2641 ataatcagat taaattcctg tttgattcac gaggttttcc actggcgtat attacctggg
     2701 catatcttga ggctgataca gaagcacgcc tgctcaggga tccagaattc aggttgcatc
     2761 cgtctgaatg gaatgaagat ggaaggatct ggattctgga tttctgttgt aaaccaggct
     2821 ttggtcgaaa agttattgac tatctcatac agcttcagcc atggggagaa ggagaagtac
     2881 gatggttaag caggcgaaag aaaattgtga catacatccc tgagcggctg cataaaacgt
     2941 agtacctctg aagatacaga gataattgta aattacgggg taaatgcatc gctgatacta
     3001 ttttgacagg actctgtatt ttctggcttt gattatagca tttcggttaa ccagtgaagg
     3061 tattacagga aaagtgtgag taagaggagc tattttgttt cgtcaggatg ctttagaaaa
     3121 cagaaaaatg aagtggcagg gacgggcaat attacttccc ggaataccac tatggttaat
     3181 catgctggga agcatagtgt ttattacggc atttctgatg ttcattattg ttggtaccta
     3241 tagccgccgt gttaatgtca gtggtgaggt cacaacctgg ccaagagctg tcaatatata
     3301 ttcaggtgta cagggatttg ttgtcaggca gtttgttcat gaaggacagt tgataaaaaa
     3361 aggggatcct gtttatctga ttgacatcag taaaagtaca cgtagtggta ttgtcactga
     3421 taatcatcgt cgggatatag aaaaccagct ggttcgtgtg gacaagatta tttcccgtct
     3481 ggaagaaagt aaaaaaataa cgctggatac cctggaaaaa caacgcctgc aatacacaga
     3541 tgcgtttcgt cgctcatcag atattataca gcgtgcagag gaagggataa aaataatgaa
     3601 aaacaacatg gagaattaca gaaactatca gacaaaaggg ctgattaata aagatcagtt
     3661 aactaaccag gtggcattat attatcagca acaaaacaat cttcttagcc tgagcggaca
     3721 gaacgaacag aatgccctgc agataaccac tctggagagt cagattcaga ctcaggcagc
     3781 agattttgat aaccgtatct accagatgga actgcaacgg tacgagttac agaaagaact
     3841 ggttaacact gatgtggagg gcgaaatcat tatccgggcg ttgactgacg ggaaagttga
     3901 ctccctgagt gtcactgtcg ggcaaatggt caatcccgga gacaaccttc tgcaggttat
     3961 tcctgagaac attgaaaatt attatcttat tctctgggtc ccgaatgatg ctgttcctta
     4021 tatttcggct ggtgacaaag tgaatattcg ttatgaagcc tttccggcag aaaaatttgg
     4081 gcagttttct gctacggtta aaactatatc caggactcct gcatcaacac aggaaatgtt
     4141 gacctataag ggagcaccac agaatacgcc gggcgcctct gttccctggt ataaagtcat
     4201 tgcgatgcct gaaaagcaga ttatcagata tgacgaaaaa aacctccctc tggaaaatgg
     4261 aatgaaagcc gaaagtacac tatttctgga aaaaaggcgt atttaccagt ggatgctttc
     4321 tcctttctat gacatgaaac acagtgcaac aggaccgctc aatgactaac gggaatttca
     4381 gacaaattat aaatcagctt gatatgcgct ggcgacgtcg tgttccggtt attcatcaga
     4441 cggagaccgc tgaatgtgga ctggcctgcc tggcaatgat atgcggtcat tttggtaaga
     4501 atattgacct gatatctctt cgtcgtaagt ttaatctctc ggcccgtgga gcaaaccttg
     4561 caggaatcaa tggaatagcg gagcagctgg ggatgatcac ccgggctctt tcactggagc
     4621 tggatgaact tggtgccctc aaaatgccgt gtattctcca ctgggatttc agtcacttcg
     4681 tcgtgctggt cagcgtaaag cgtaaccgtt atgtactgca tgatccggcc agaggaagaa
     4741 gatatctcgg tcgggaggaa atgagccggt attttacggg cattgcactt gaggtctggc
     4801 ccggaggtga attccggacg gaaacccagc aaacccgcat aagtctccgt tcactgatta
     4861 acagtattta cggtattaaa agaacactgg cgaaaatttt ctgtctgtca gctgtaattg
     4921 aagcaatcaa tctggtaatg ccggtgggaa ctcagctggt tatggatcat gcgattccgg
     4981 cgggggaccg agggctgctg acgcttattt ctgctggcct gatgttcttt atattgctca
     5041 gggccgcggt gagtatgctg cgtgcatggt cctcactggt tatgagcacg ctcatcaata
     5101 tacagtggca gtcgggtctg tttaaccacc ttctcagact gccgctggca ttttttgaac
     5161 gccgtaaatt aggtgatatc cagtcgcgtt ttggctccct tgacactttg agggccacct
     5221 ttaccacctg tgtggttggg gcaatcatgg acagtattat ggttgtgggt gtttttgtga
     5281 tgatgctgtt atatggagga tatcttacct ggatagttct cggttttacc atggtttacg
     5341 ttcttattcg tctggtgaca tacggctatt accggcaaat atcggaagaa actcttgtca
     5401 ggggggcccg ggccagctcc tattttatgg aaagcctgta tggtattgcc acggtaaaaa
     5461 tccagggtat ggtcgggatc cggggaacac actggcttaa cctgaaaata gatgcgatca
     5521 attcaggtat taagttaacc aggatggatt tgctcttcgg gggaataaat acttttgttg
     5581 ccgcctgtga tcaggtggcg attttatggc tgggtgcaag ccttgtgatc gataatcaga
     5641 tgacaatagg gatgtttgtg gcatttggtt cttttcgtgg gcagttttcg gatcgggttg
     5701 cttcgctgac cagttttctt cttcaactga gaataatgag tctgcataat gagcgcattg
     5761 cagatattgc actacatgaa aaggaagaaa agaaaccgga aattgaaatc gttgctgaca
     5821 tgagcccggt ttcactggaa accactgatt taagctaccg gtatgacagc cagtcagcac
     5881 aggtattcag tggtctgaat ttgtctgtgg ctccgggaga aagtgtggct ataactggtg
     5941 cctccggtgc cggaaaaacc acattaatga aagtattatg tggactgttt gaaccagata
     6001 gtggaaaagt actggttaat ggcacggata tacgtcaact tggaataaat aattatcacc
     6061 gtatgatagc ctgtgttatg caggacgacc ggctattttc aggatcaatt cgtgaaaata
     6121 tctgtgggtt tgcagaagaa acagacgacg aatggatgac agaatgtgcc agagcaagtc
     6181 atattcatga tgtgataatg aaaatgccaa tggggtatga aacgttaata ggtgaactgg
     6241 gggaaggtct ttccggcggt caaaaacagc gtatattcat tgcccgagct ttataccgga
     6301 aacctggaat attatttatg gatgaggcta caagttctct tgatacagaa agtgaacgtt
     6361 tcgttaatgc tgccataaaa aaaatgaata tcacccgggt gattattgca cacagagaaa
     6421 ctacgttgag aactgttgac aggattattt ctatttaaaa tccactggtg taactttgta
     6481 aggagttttg tcgatggggg aggtcaagaa ggatataaaa ataacagtga ttgcttttgt
     6541 tatcaattat ctgttctttt atattccggt gtcattatat cttagttatt actatggata
     6601 taattttttt aatctatata tgtttttttt atcacttgta gttacatttt tatcgttgtg
     6661 gttaaacgtg aatttttact tcttcacaaa tcttatagcg aaggtgttga aatgagaaaa
     6721 ctatctgaaa atgaaataaa acaaatatct ggaggtgacg ggaatgacgg gcaggcagaa
     6781 ttaattgcta ttggttcact tgctggtacg tttattagcc cgggatttgg ttctattgca
     6841 ggggcttata taggtgataa agtacattca tgggcaacga ctgcgacggt tagtccctcc
     6901 atgtctccct caggtatagg attatcatcc cagtttggat ccggcagagg tacatcaagt
     6961 gcctcttcgt ctgcggggag tggaagttaa accttatatt gttaatgaag cacctgttac
     7021 aggtgcttca ttatatgata atggtgccta tgtaacgctt taagttaggc tcctccgaac
     7081 gtttattatt ttatgcatac cgcatagtaa caatgccagt ccgtttaatt tttatcatat
     7141 gagattgtaa cagaaaatgc tctgttaatg agtataactg atagaagcca gatattgtat
     7201 tcctgctatg actgcattca tcgacatgtg aagtaatatg ggcattaaca ggccattcga
     7261 ctttattttt gcactaataa gaactaacga taccagaaag agcatcaaaa atgtatgaaa
     7321 gtcagtatat tgtagatgta atgcagaaaa tatgactgat gttacaatag cagaggtgta
     7381 aatattgtca ttaaaccaga atttaaaaga gttaaaaaga caccctcgaa atacaatctc
     7441 ttcatataca ggaacaagaa gtacagaaga aaatatatta atccataaaa tggctccgga
     7501 aaaagacatt tgcgagatca tccattcttc cgttttcctc actgccagca gataagggac
     7561 aaataactga ataatcatta ttaaagagaa aagagtaaaa aaaacatcca gtcgaaaaga
     7621 acctcttcct aatccttctc tttttcggaa gaaaataaaa tacagtggaa caagaattag
     7681 aaattcagca agaaataatg ctggaacaag aagtcccctg gatataagtt cctgcctgtt
     7741 tgtaagaaat gcaggaataa aggtaataga aaatgataaa gtaaacattg aaaagcagcc
     7801 ggctgatgac tgttttttta tagttgcatt tttatttttt gtcatttcca tttatattcc
     7861 cttacttgtt gtttaaaatt cttttatata acaggaagag gtaaccatag aatgaacgtt
     7921 tattttcctg tatcagaaca gccataactt ttattttata gttgttatca gtgaatgaat
     7981 gcaaactaat agaataatga gaaaaacaat ggagaaaaaa cgatggaccg gacgccatag
     8041 ttcatattct cgtgtatcag cctcacgtat caacctttct gaaatggttt cggtttcctt
     8101 acctcgttcc agaatatgtc tgtaatattt cctcatgaca tataccgaaa actgaaacca
     8161 gattaatatg gatggacaca caacacataa acttctgatg atatcacccg ggaaagtatc
     8221 ttgggcgggt aaaagaaaat catatactct gaataaaatc atcatcgaga taaatgagac
     8281 gccagcacct atccagtatt cgatatggga agatgcagca ttatatgcgt cagtaaaatt
     8341 tttctttctt aatgaaggtg gaagtgctga atgttcagga atgctttttc ttgtccagta
     8401 tattttcata tagcaactca t
//
