LOCUS       BGC0000590              5263 bp    DNA     linear   BCT 25-OCT-2010
DEFINITION  Escherichia coli microcin N producer system gene cluster, complete
            sequence.
ACCESSION   BGC0000590
VERSION     BGC0000590
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
COMMENT     ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2022-11-07 19:57:30
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5263
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..5263
                     /db_xref="taxon:562"
                     /mol_type="genomic DNA"
                     /organism="Escherichia coli"
     subregion       1..5263
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="RiPP"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..5263
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     CDS             90..524
                     /codon_start=1
                     /note="H-NS microcin N regulator"
                     /product="McnR"
                     /protein_id="ACP43448.1"
                     /transl_table=11
                     /translation="MSELTKEDEYGIISRTMMNIRSLRVFAREIDFEQLLEMQEKLNVV
                     IEERREDAEREAAERAERERKRQELLQLIAGEGFSPEELLGLSEEAPKSRKKTLPKAPP
                     KYQFEENGETKYWSGRGRAPKPIAEALAGGRSLDEFLIEK"
     misc_feature    90..5230
                     /note="microcin N producer system gene cluster"
     CDS             827..1132
                     /codon_start=1
                     /note="microcin N immunity"
                     /product="McnI"
                     /protein_id="ACP43449.1"
                     /transl_table=11
                     /translation="MKRNKLTRMSFLNFAFSPVFFSIMACYFIVWRNKRNEFVCNRLLS
                     IIIISFLICFIYPWLNYKIEVKYYIFEQFYLFCFLSSLVAVVINLIVYFILYRRCI"
     CDS             1129..1398
                     /codon_start=1
                     /note="microcin N precursor"
                     /product="McnN"
                     /protein_id="ACP43450.1"
                     /transl_table=11
                     /translation="MRELDREELNCVGGAGDPLADPNSQIVRQIMSNAAWGAAFGARGG
                     LGGMAVGAAGGVTQTVLQGAAAHMPVNVPIPKVPMGPSWNGSKG"
     CDS             1840..3117
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1029:RND family
                     efflux transporter MFP subunit (Score: 75.2; E-value:
                     7.5e-23)"
                     /gene_kind="transport"
                     /note="accessory protein of the microcin N ABC transporter
                     system"
                     /product="McnA"
                     /protein_id="ACP43451.1"
                     /transl_table=11
                     /translation="MFRQEATVYRKMKWCGRAVLLPGIPPWLVISVSVFFFISFIVFVM
                     EGTYTRRINVEGEITTWPRPVNVYSGVQGVVIKQFVTEGQRIKKGDPIYLIDVSKSTSS
                     GVVGDNKRRDIEKQLSRIGDIISRLEENKKTTLQTLEKQRIQYYSAFERSTEILRRAEE
                     GVKIMKSNMDNYKQYQTKGLINKDQLTNQIALYYQQQNNILSLSTQNEQNLLQVTSLES
                     QMQTLAAEFDNRIYQVELQRYELQKELVDTDAGRDIIIRALSDGKIDSLSVTPGQMVSV
                     GDSLLQIIPEEIKNYHLIVWVPNNAIPYISVGDNVNVRYEAFPPEKFGQFTAKIMLISR
                     TPASAQEMQTYPGAPRNNTGVSVPYYKIVLNPEQQTIEYGEKKMPLENGMKAQSTLFLE
                     KRKIYQWMLSPFYNMKYSAVGPVNEQ"
     CDS             3107..5230
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288:ABC
                     transporter related protein (Score: 252.3; E-value:
                     1.6e-76)"
                     /gene_kind="transport"
                     /note="ABC transporter of the microcin N ABC exporter
                     system"
                     /product="McnB"
                     /protein_id="ACP43452.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 5.8e-45, bitscore:
                     140.8, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNNATSPLNTLLNKLEIGLRRRIPVVHQTESSECGLACLSMICG
                     HYGRHIDLSTLRRQFNLSALGTTLAGITEIGSQLGMETRAFSLDLNELSVLKLPCILHW
                     EFSHFVVLVSVRKNHFVLHDPARGRRTVGLAEMSQCFTGVALEVWPGTEFVQETMKNRV
                     VLRTLFRSIYGLRSTLTKIFCFSLVIEAVGLVIPVGTQLVMDHAIPAGDRGLLSLICVG
                     LMFFILLRTAVSMIRSWSSLVMETLINVQWQSGLHRHLLQLPLAYFERRKMGDIQSRFS
                     SLDTLRTTFTTSVVGAIMDSIMVSGVLAMLVLYGGWLTTIVLGFTIIYVLIRLLTYNYY
                     RQLSEESLIREARASSYFMETLYGIATIKMQGMGERRGRHWLNLKIDAINTGIRLARMD
                     MLFSGINTFVAACDQVVILWLGTSLVIDNQMTIGMFVAFGVFRGQFSDRVGSLTNFLLQ
                     LRMMSLHNERIADIAMNEREARKPDTAMKADMYPVALETQDLSFRYDSQSAPVFSNLNI
                     SIKPGESVAITGASGSGKTTLMKVLCGLLVPESGRVMIDGTDIRSLGVNNYHKIISCVM
                     QDDRLFSGSIRENICGFTENIDEAWMVECARASFIHDVIIKMPMGYDTLIGELGEGLSG
                     GQKQRIFIARALYRRPGILFMDEATSALDTESEYYVNQAIKQLNITRIIIAHRETTVKS
                     ADRIILLEAPARI"
ORIGIN
        1 accgccgtgc agaactggtg atgaataaac tttatgataa ggttccttcc ggcgtatgga
       61 agtacgtcca ttaaacaaga ggattaatta tgagcgaact gactaaagaa gatgaatacg
      121 gcattatcag ccggactatg atgaatattc gttcattgcg tgtgtttgcc cgtgagattg
      181 attttgagca gttgctcgaa atgcaggaaa agctcaacgt tgttattgaa gaacgtcgtg
      241 aagatgctga acgtgaagcg gctgaacgag cagagcgtga acggaaacgt caggaactgc
      301 ttcagttaat cgccggagag gggttctcac cggaagaact gcttggtctg tctgaagaag
      361 caccaaaatc acgtaaaaaa acgttaccaa aagcgccgcc taaatatcag tttgaagaaa
      421 atggtgaaac gaaatactgg tctggtcgtg gacgtgcgcc aaaaccaatt gctgaagcac
      481 tggcaggtgg tcgttctctt gatgagtttc tcatcgagaa atgattctac agccctcctt
      541 ttctctggag ggctgtaccc tttaaagcac acttgccagc caatcttgta taaatgttat
      601 gtttgaactg gatatcaaat tttcgggcag agctacagct ctcggtagtc tgacagattg
      661 actccagttt gtggtttcca tggggtggct ggtacaacaa ccgtcggttg ctgatcagac
      721 acgccaccaa cagatttatc tgctggccag tatctttttt tgggaactat atcacaaaaa
      781 gatgcttgat aatgatttgc attattaagt atagtgtaaa aaattcatga agcgtaataa
      841 gttaacaaga atgagttttc ttaattttgc attttctcct gtattcttct ccattatggc
      901 gtgttatttc attgtatgga gaaataaacg aaacgaattt gtctgcaata gattgctatc
      961 aattataata atatcttttt tgatatgctt catatatcca tggctaaatt acaaaatcga
     1021 agttaaatat tatatatttg aacagtttta tcttttttgt tttttatcgt cactcgtggc
     1081 tgttgtaata aacctaattg tatactttat attatacagg agatgtatat gagagagtta
     1141 gatagagagg aattaaattg cgttggtggg gctggagatc cgcttgcaga tcctaattcc
     1201 caaattgtaa gacagataat gtctaatgcg gcatggggcg ccgcctttgg tgccagaggc
     1261 ggtttagggg gaatggctgt tggagccgca ggtggggtta cgcagacagt tcttcaagga
     1321 gcagcagctc atatgccggt taatgtccct atacctaaag ttccgatggg accctcatgg
     1381 aacggaagta aaggataaga ttcattaagg tagaatattt aactttgctt gggtgttata
     1441 tcactcaggt aaagttatta ctctattcat cagataattg gctaatcttt tttaacaagt
     1501 aattctctat ggtaatcgtg tacagacact atagctctca tggatttgct ccctctcctc
     1561 tgattctccc cagactgtat ttgtcgtgta agttcggata aggagaatag gatcacctgg
     1621 cgcactattt tgaactccat cagtagccac atgaccgttg aaagagccaa agggaagcaa
     1681 ctaaagctgt ctgataaaca aaaaatttgt aatggatgca cgaaaccggg ctgcactggt
     1741 tgaattattt ccagtctaac gaccaattgt ctatagaaca ccgattcata ataatataat
     1801 tattttttac catatcatca gtcagtaagg aaagaaaagg tgtttcgcca agaagcaaca
     1861 gtatacagga aaatgaagtg gtgcgggcgg gcagtattat tgccgggaat tcccccctgg
     1921 ttggtcatat cagtaagcgt ttttttcttt atttctttta tagtttttgt tatggaaggt
     1981 acatacacac gccgcattaa tgtagaagga gaaatcacta catggccccg acctgtaaat
     2041 gtctattcag gtgtacaggg agttgtcata aaacagttcg tcactgaagg acaaagaatc
     2101 aaaaaaggcg acccaattta tctgattgat gtcagtaaaa gtactagtag tggtgttgta
     2161 ggtgataaca agcggcgaga tatcgaaaag caactgtccc gcattgggga tatcatatcc
     2221 cgccttgaag aaaataaaaa aactacacta cagacactgg aaaaacaacg gattcagtac
     2281 tactcagctt ttgaacgttc gactgaaatc cttcgtcgtg ctgaagaagg agtaaaaata
     2341 atgaaaagta atatggataa ttataaacaa tatcaaacga aaggactcat aaacaaagac
     2401 cagttaacca accagatagc gttgtattat cagcagcaaa ataacatatt gagtctgagc
     2461 acacaaaatg aacaaaactt attacaggta accagtctgg aaagccagat gcagacccta
     2521 gcagcggaat ttgacaaccg tatttatcag gtggaactgc agcgttacga attacagaaa
     2581 gagctggttg atacagatgc tggaagagat attattattc gcgctttgtc agatgggaaa
     2641 attgattcgt tgagtgttac accggggcag atggtcagcg taggcgacag tctgttacaa
     2701 attattccag aagaaataaa aaattaccat ctgattgttt gggtacccaa taatgccata
     2761 ccttatattt ctgttggtga taacgttaat gttcgttatg aggcctttcc gccagaaaaa
     2821 tttggccagt ttacagcaaa aataatgtta atatccagaa ctccggcctc cgctcaagaa
     2881 atgcagacct atcccggagc cccaagaaat aatacaggtg tttctgtgcc ttactacaaa
     2941 atagtgctaa atccggagca acaaacaata gaatacggcg aaaaaaaaat gcctctggag
     3001 aatggtatga aagcacagag tactttattt ctggaaaaga ggaaaatata ccagtggatg
     3061 ctatccccat tctataacat gaaatacagt gcggtggggc cagttaatga acaataacgc
     3121 tacctccccc ttaaatactc tcttgaacaa actagaaata ggactacggc gtcgcattcc
     3181 ggtcgtgcac cagactgaat catcagaatg tgggctggcc tgtctgtcga tgatatgtgg
     3241 ccactatggc aggcatattg atctgagtac tctgcgtcgc cagtttaacc tgtctgctct
     3301 gggaacaact ctggctggca ttacagaaat aggatctcaa ttgggtatgg aaacccgagc
     3361 attctcactg gatctaaatg aactcagtgt tctgaagttg ccctgcatcc ttcactggga
     3421 gtttagccat tttgtggtgc tggtcagcgt cagaaaaaat catttcgttc tccatgatcc
     3481 ggcacgagga cgtagaacgg taggacttgc tgagatgtca cagtgtttta ccggtgtcgc
     3541 gcttgaagtc tggcccggca cagagtttgt tcaggaaacc atgaagaacc gggtagtgct
     3601 tcgaacgctg tttcgcagca tttatggtct ccggagtacg ttgactaaaa ttttttgctt
     3661 ttcactggtg attgaagctg taggtctggt gatacctgtt ggcactcaac tggtgatgga
     3721 tcacgccatt cctgcaggag atcgaggcct gctctccctc atctgcgtcg gtctgatgtt
     3781 tttcatcctg ttgcgcaccg cggtcagtat gattcgctca tggtcgtctt tggtaatgga
     3841 aacgctaata aacgtgcagt ggcagtcggg gttgcaccgg catctgctgc agttgccgct
     3901 ggcgtatttt gaacggcgaa agatgggtga tattcagtcc cgcttcagtt ctcttgacac
     3961 actgcgtacc acgtttacaa ccagcgtggt cggagcgatt atggacagca tcatggtttc
     4021 cggagtcctg gctatgttgg tgttgtacgg tgggtggttg acaactattg tgctgggttt
     4081 caccattata tatgtgctaa ttcgtttact gacctataac tattatcgcc agttgtcgga
     4141 agaatccctg ataagggaag cacgtgccag ttcgtatttt atggaaaccc tgtacggaat
     4201 agccacaatc aagatgcagg gaatggggga gcgcagaggt cgtcactggc tgaatctgaa
     4261 gattgatgca atcaatactg gtataaggct tgccaggatg gatatgcttt tcagtgggat
     4321 taacacgttt gttgcagcat gtgaccaggt cgtgattctg tggctgggaa ctagcctggt
     4381 gattgacaat cagatgacca tcggaatgtt tgtggcgttc ggggtattcc gcgggcaatt
     4441 ctcggaccgt gttggttccc tgacaaattt tctgcttcag ttgcgtatga tgagtcttca
     4501 taatgaacgt attgctgata tagccatgaa cgagcgggaa gcccggaagc ctgatacggc
     4561 catgaaagcc gatatgtatc cggtagcact ggagacccag gacttgagct tccgttatga
     4621 cagtcagtct gctcccgttt tcagcaacct gaatatcagt ataaaaccgg gagaaagtgt
     4681 ggcaattacc ggggcctcag gttcagggaa aaccaccctg atgaaggtac tgtgtgggct
     4741 gcttgtaccc gaatccggca gagtcatgat agacggtacc gatatcagat cgttgggggt
     4801 caataattat cacaaaatta tttcctgtgt gatgcaagat gaccgactgt tttcaggctc
     4861 gattagagaa aatatttgcg gtttcactga aaatattgat gaggcatgga tggtggaatg
     4921 tgccagagct agctttattc acgacgttat cataaaaatg cctatgggtt atgatacact
     4981 gataggtgaa ctgggtgaag gtctttctgg cgggcagaaa cagcgaattt ttattgctcg
     5041 agcgctttac cgtcgaccgg gcattttatt catggatgag gccacaagtg cgctggatac
     5101 tgaaagtgaa tattatgtta atcaggcgat aaagcaactg aatataacac gaataatcat
     5161 cgcacacaga gaaacaacag taaaatcagc cgacaggata attttactgg aggcgcctgc
     5221 gaggatataa ctaaaataat gatatttatt actcaagggt aga
//
