LOCUS       BGC0000593              6678 bp    DNA     linear   BCT 27-APR-2008
DEFINITION  Microcystis aeruginosa MRC microviridin biosynthetic gene cluster,
            strain MRC.
ACCESSION   BGC0000593
VERSION     BGC0000593
KEYWORDS    .
SOURCE      Microcystis aeruginosa MRC
  ORGANISM  Microcystis aeruginosa MRC
            Bacteria; Cyanobacteria; Oscillatoriophycideae; Chroococcales;
            Microcystaceae; Microcystis.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-07 19:58:00
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6678
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..6678
                     /clone="mdnMRC"
                     /db_xref="taxon:507735"
                     /mol_type="genomic DNA"
                     /organism="Microcystis aeruginosa MRC"
                     /strain="MRC"
     protocluster    1..6678
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[241:391]"
                     /cutoff="20000"
                     /detection_rule="(mvd or mvnA)"
                     /neighbourhood="10000"
                     /product="microviridin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      242..391
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(mvd or mvnA)"
                     /neighbourhood="10000"
                     /product="microviridin"
                     /protocluster_number="1"
     cand_cluster    1..6678
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(mvd or mvnA)"
                     /kind="single"
                     /product="microviridin"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..6678
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000593"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..6678
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="microviridin"
                     /region_number="1"
                     /rules="(mvd or mvnA)"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            242..391
                     /gene="mdnA"
     CDS             242..391
                     /codon_start=1
                     /db_xref="InterPro:IPR022217"
                     /db_xref="UniProtKB/TrEMBL:B2G3C8"
                     /gene="mdnA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     microviridin: mvnA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     microviridin: mvd"
                     /gene_kind="biosynthetic"
                     /product="microviridin precursor"
                     /protein_id="CAQ16121.1"
                     /sec_met_domain="mvnA (E-value: 3.4e-29, bitscore: 88.5,
                     seeds: 16, tool: rule-based-clusters)"
                     /sec_met_domain="mvd (E-value: 2e-14, bitscore: 41.5,
                     seeds: 55, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAYPNDQQGKALPFFARFLSVSKEESSIKSPSPDHEISTRKYPSD
                     WEEW"
     misc_feature    242..2678
                     /note="microviridin biosynthetic gene cluster"
     gene            668..1648
                     /gene="mdnB"
     CDS             668..1648
                     /codon_start=1
                     /db_xref="GOA:B2G3C9"
                     /db_xref="InterPro:IPR013651"
                     /db_xref="UniProtKB/TrEMBL:B2G3C9"
                     /gene="mdnB"
                     /product="ATP grasp ligase"
                     /protein_id="CAQ16122.1"
                     /transl_table=11
                     /translation="MKESPKVVLLLTHSGDFFTIDRVAEAIEKKGATPFRLDTDKFPLE
                     VQLTAQFNGKKSFYQLSYNHQSIDSEQVQSVWTRRIWQPELTGDLDPQFREVCVRESQT
                     TLAGFWDSLRSARWLDNLAQIEKAKNKLLQLRLASEVGLIIPPTLVTNNPDAAREFFSQ
                     VQGRMVSKLLTAIARSMESPEFFLYTSRVKAEDLEEAESLRYCPMVFQAEIPKQLELRV
                     VVVNGQTFVGALESSQYNNSAVDWRRPGIDPGAWQHHTLPDSLLQQLQIFMANLGLNFG
                     AFDFILTPGGEYVFLEVNPGGEWGMLERDLDLPISQAIADFLVFG"
     gene            1704..2678
                     /gene="mdnC"
     CDS             1704..2678
                     /codon_start=1
                     /db_xref="GOA:B2G3D0"
                     /db_xref="InterPro:IPR013651"
                     /db_xref="InterPro:IPR013816"
                     /db_xref="UniProtKB/TrEMBL:B2G3D0"
                     /gene="mdnC"
                     /product="ATP grasp ligase"
                     /protein_id="CAQ16123.1"
                     /transl_table=11
                     /translation="MTVLIVTFSRDNESIPLVIKAIEAMGKKAFRFDTDRFPTEVKVDL
                     YSGGQKGGIITDGDQKLELKEVSAVWYRRMRYGLKLPDGMDSQFREASLKECRLSIRGM
                     IASLSGFHLDPIAKVDHANHKQLQLQVARQLGLLIPGTLTSNNPEAVKQFAQEFEATGI
                     VTKMLSQFAIYGDKQEEMVVFTSPVTKEDLDNLEGLQFCPMTFQENIPKALELRITIVG
                     EQIFTAAINSQQLDGAIYDWRKEGRALHQQWQPYDLPKTIEKQLLELMKYFGLNYGAID
                     MIVTPDERYIFLEINPVGEFFWLELYPPYFPISQAIAEILVNS"
ORIGIN
        1 gagagcaggg agcaaaggat ttagtatgat ttagcggaaa tttttcttaa atttttctta
       61 agtgcttgac attggccgga ttagtgctta caatgttttc catcacccaa ataaaagcaa
      121 attatttgca caataccgat tctgaaagcc atgcacagca aagctttcag tggttagtac
      181 aggtggttgt ttttgtctga gtgattaaac ccattcactc gaaattacca gaggaataac
      241 tatggcatat cccaacgatc aacaaggtaa agcacttcct ttctttgctc gtttcttgtc
      301 cgtaagcaaa gaggaatctt ccatcaagtc tccttcccct gatcatgaga tttcaacacg
      361 caaataccct tctgactggg aagagtggta aactcagatg acttgagtaa ttgcagcact
      421 tttcttgatt acaccgtaca gacaaaaagc taaaagctcc actaagcaag gcttgcagcc
      481 aaaaaactat acgttgagat tctgggaagt gctgtcaact aaatttagta attacttaag
      541 aaaaaaccaa tactgcaata agtcaagcaa aagcaagact ttttgcagca ttttattgag
      601 attccgatca ataaaaacta acccaaaagc cttaagatgg gttaaaatgt aaggacttgc
      661 aactgatatg aaagaatcgc ctaaagttgt tttattgttg acccatagcg gcgatttttt
      721 cactattgac cgcgtggctg aggcgataga aaaaaaagga gccacacctt ttcgcttaga
      781 taccgataaa tttcccttag aggttcaact aacagcccaa tttaatggga aaaaaagttt
      841 ttatcaatta agctataacc atcaatctat tgatagtgaa caggtgcaat ctgtttggac
      901 aagacgtatt tggcaacctg aactaacagg ggatttagat cctcagtttc gggaggtttg
      961 tgtgcgagaa tcccagacga ctttggctgg tttttgggat agtttaaggt cagcgcgttg
     1021 gttagataat ttagcgcaaa ttgagaaggc taaaaataag ttattacaac ttcgtttggc
     1081 ctcagaagtt ggcttaatta ttccccccac tttagtgacc aataatcctg atgctgcccg
     1141 ggagtttttt tcccaggttc agggacgaat ggtgagtaaa ttgttaactg cgatcgcccg
     1201 cagtatggag tcgccggaat tttttcttta taccagtagg gtgaaagcag aagaccttga
     1261 ggaagcggaa tctttacgct attgtcctat ggtttttcaa gcggaaattc ccaaacaatt
     1321 agaattgaga gttgtcgtgg ttaatggaca gacatttgtg ggagctttag aatcttctca
     1381 gtataataat tctgccgtag attggcgaag accgggtatt gatcctggtg cttggcaaca
     1441 tcatacttta cctgattcac tattgcagca acttcagatt tttatggcta atttagggtt
     1501 gaattttggg gcgtttgatt ttatcttaac tcctggggga gaatacgttt ttttagaagt
     1561 taatccaggt ggtgagtggg gaatgttaga acgggattta gatttaccta tttctcaggc
     1621 tatcgccgat tttttagtct tcggttgatc ggcaaagctg gtgctcaaaa aaaattgtta
     1681 atcctaagtt ttcaaggaga tttatgaccg ttttaattgt tacttttagc cgcgataatg
     1741 aaagtattcc tctggtaatc aaagccatag aagccatggg taaaaaagcc ttccgttttg
     1801 ataccgatcg cttccctaca gaggtgaaag ttgatcttta ctcaggcggt caaaaaggcg
     1861 gaattattac cgatggagac caaaaattag agctaaaaga agtttctgct gtctggtatc
     1921 gacgcatgag atacggacta aaattacccg atgggatgga tagtcaattt cgcgaagctt
     1981 ctcttaagga atgtcggtta agtattcgag gaatgattgc tagtttatct ggctttcatc
     2041 ttgatccaat tgctaaggta gatcatgcca atcataaaca attgcagtta caagtggcgc
     2101 gacaattagg tttattaatt ccggggactt taacttctaa taatcctgaa gctgtcaagc
     2161 aatttgctca ggagtttgaa gcgacgggaa ttgtgactaa aatgctttct caatttgcta
     2221 tttatggaga caagcaagag gaaatggttg tttttaccag tcctgttact aaggaagact
     2281 tagataattt ggaaggtttg caattttgtc caatgacttt tcaggaaaac attcctaaag
     2341 ctttggaatt acgcatcact atcgtcggtg aacaaatatt tacggcggcg attaattccc
     2401 aacaattaga cggtgctatc tacgattggc gaaaagaggg aagagcgctc catcaacaat
     2461 ggcaacccta cgatttaccg aaaactattg aaaagcaact actagaatta atgaaatatt
     2521 tcggtctcaa ttatggtgca attgatatga ttgtcacacc agatgaacgt tatatctttt
     2581 tagaaattaa tcccgttggc gagtttttct ggctagaact ttatcctcct tattttccca
     2641 tctcccaggc gatcgctgaa atcctagtta actcataaac caacaaatac agagccagag
     2701 tattttatcg catcgacgag gctctgactc cagatttccc ccccgactcc tgacttgaaa
     2761 gagggtgtaa agaaggaggt tgataaatca ttaactgtcc ataaactact gacaacgctg
     2821 ggacttcctg caaacagaaa accactgggt aaaccgatat attcatcgct gatagagttt
     2881 aaatcaatta agccagtttc gcaagcggaa agggtgataa ggcgacattt atgcaagcgc
     2941 agttcaaaga tttcgcctaa tgttaagggt tccttattgg ctaataataa ggcggattca
     3001 aggggattat cggggttaaa gcttccatga caggagaagt gataacagtg gttatctgag
     3061 gatttgagat ggggaagaac agcgttttta gtggcgttat cttgggcgat aacttgaggt
     3121 tcagtaaaga aagttgagag gatattaact tctaagtcag tgtaaaataa gtctttggtg
     3181 gggttttgaa tggcgaacag tttattaaac tcggaatggt gaaaagtttg ggagattttt
     3241 tgcaagattt gacaactagg ggcatattgt acacccttcg gaaataaatc ttgtaattct
     3301 gatccccccg ttgccgactc cccacttttt aaggggggtt gggggggatc aacaggtaag
     3361 gcgtgtaagg gtaaaatatg cagaaaccaa tgggggataa taattaagcg ttgacaagta
     3421 tcgggaatca gggcaagaat atcattaata tgcagaatat cagcaaaagt ctgtaagagg
     3481 ttagacagtt gatttctcca ttcttgcttg cgattgtcgg agtagtaaag ctgtagataa
     3541 ttgttgattg tatcaaataa ttgctttaca tcatcctcgg aagattgcca aactttaacc
     3601 tctccctcag cagacacgac aaaagtgagt attttctccc ttgtaatgta ccattgcagc
     3661 agacaagttt cggcatcagt taaagcctga atctctgtaa atacgatcgg ctcaactttt
     3721 tgagtgaggc taaagagagg atctttaatt tctcttgcaa taaaattatc taagtcttgt
     3781 tgatattctt tcagataact gtgatctgga acgtaaggag ttaagttatc actacgcatt
     3841 aaattctggt taatgctttg atggtgtaag cgaatttttt cattaaccac tctttgcttt
     3901 aattcattta attgggcgat aatttcttga ctaacgcctt ggggttgtag gtttttctgg
     3961 gtgattaatt ccaccagatt acgcccttta ctgcgttcga tatattctaa tgccttctca
     4021 ggctgattga ggttaagatg cgcctgtaca atgcgatgga aaacctcaat agcataagag
     4081 agggtttgtt cacgactgcg ggggttaagt gcctctaagc gagcattttc gacggcttca
     4141 atggcgatat gataggcttc tgtggcaggt tgccattgtt tttctaaata atgtaaatta
     4201 ccaagattag tagcagtctc taaacacttt aatggatcat tatctttggt acgaaatttt
     4261 aaagcatctt tataggctaa aattgccttg tttaaatttt ccgcccttcc ttctttaatt
     4321 ttccgagaat aagtaaagta agcaccaccg agattatttt gagtttttgc ccattcataa
     4381 ggataagatt caaaagttct cacctgtaac gcttgttgat aatgaaaaat agacatttct
     4441 aaattactaa tttcatcttc actcattctt tttgcataag ctgatgcaag attatcttgt
     4501 aaatttgccc attcaatagg attattattt tgtggacata cctctaaagc attttgaaaa
     4561 gcaacaatgc ctttttctat ttccttattt tgatcttctt cagaaatgaa cagataaaga
     4621 ttaaatgtat gaataatacc aagattattt tggatcttaa tccactcttg agggaagttt
     4681 tcacgattac atattcttag ggcattttca aaacactcaa ctgctttgtt aaaattatct
     4741 agtttatttt ttttttcttt gactagatta attctgtcac ggtaagcatt tcccatatta
     4801 ctctgtacca ttgcccattt atagtgagat tgaggaactt taagagcttc taaatgagca
     4861 gagatagctc tctctagatt atcagcttta tttcccttta tcctgtgact ataagctacg
     4921 ccaagattat cttgtattgt cgcccattct tgaggagaat tagtaaaagt tataatttgg
     4981 agagtttctt catatttttt gatagctatt tccaatttgt cagcataatc ttctccagtt
     5041 ttgttgcggt aaacagccgc tagatttgct tgtattctag cccattgtag cggtgatttt
     5101 ttacggttat atactcttaa gacattttca aaagctgtta tggcttctgc taaattacta
     5161 tttttatctc ctttagttct tttacgataa gcgttgccta gaatattttg agtatcagcc
     5221 catttatgag gattttgttc aaacttaatc tcttttaata attcattaca cacagtaatt
     5281 gccatttctt gattattagc atcatcgtaa gctatagcta gattatattt atttctagtc
     5341 cattcttcac gaaattggtc aaaagtaaat atttgcagag catttttaaa gctattaata
     5401 gctattctta aattttctga tttatttcct tgtattcgtt ctaaataagc tattccgagc
     5461 cgatcttgaa caactgccca catttcagga atagtatctt tactaaatat tcttaaggct
     5521 gtttgataac aagttatgac aatttctttg tttatatgag taattcctag agaaaaattt
     5581 gataataatt caccaaaact cataataatt atgccaaaac gttgtagtaa tacttgagac
     5641 tttgccccat tttgtaaaat attattaatc caatctgtaa aaatttgatg aaaagtttca
     5701 ttaagtttac tcagattttg gtttaggtaa ggataaagat tttcaggagt cggattctgt
     5761 tcaactaact gaagtatatg aacgataaat tgaaaaataa ttttcgtgat cattctctga
     5821 taaattaaaa atcaccattc tgcatttgtt ttaagattcc tgttaactgt tgcgccatat
     5881 tcagcaacca ttgtgcctca ttttcccttc ctgcatcccc gtattgttgc gctaccgcta
     5941 ccattacctg taccaacccc tcatccagca attcccgatt ttcctgtaat attcggggtt
     6001 catcgccatc attgcaggat aatagctgat taatcaggtt taggtatgtt tgggtgcgtt
     6061 gttcattcat agcgtggtag atggggaatt gatgggtagg ggcgaagcat tcggatagaa
     6121 aatctagggt ttcagcgata gattatgcac gaatgcttcg cccgtacagg gatttaatgg
     6181 ggagattatc agaattgtat cacagggatg ataggctaag gttattgtct ggggtagggg
     6241 cgaagcattc ggatagaaaa tctagggttt cagtgataga ttaggcacga atgcttcgcc
     6301 cgtacaggga tttaatgggg agattatcgt aattttatca cagggataat aggcgaaggt
     6361 tattagttta ttgcccgggg taggggcgaa gcattcggat aggaaattta cggtttcagc
     6421 gatatgttat gcccaaatgc ttcgcccgta cagggattta atggggagat tatcgtaatt
     6481 ttatcacagg gatgataggc taaggttatt gtctgggtag ggccaaacca ttcggataga
     6541 aaatctaggg tttcagcgga tatgttatgc ccaaatgctc gcccgtcagg gatttaatgg
     6601 gagagattat cagaattgta tcacagggat aataggcgaa ggttatttag tttattgccc
     6661 ggggtagggg cgaacata
//
