LOCUS       BGC0000602              7358 bp    DNA     linear   BCT 07-APR-2004
DEFINITION  Bacillus subtilis subtilosin gene cluster, complete sequence.
ACCESSION   BGC0000602
VERSION     BGC0000602
KEYWORDS    .
SOURCE      Bacillus subtilis subsp. spizizenii ATCC 6633 = JCM 2499
  ORGANISM  Bacillus subtilis subsp. spizizenii ATCC 6633 = JCM 2499
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 13:54:49
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7358
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..7358
                     /db_xref="taxon:703612"
                     /mol_type="genomic DNA"
                     /organism="Bacillus subtilis subsp. spizizenii ATCC 6633 =
                     JCM 2499"
                     /strain="ATCC 6633"
                     /sub_species="spizizenii"
     protocluster    1..7358
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[366:1989]"
                     /cutoff="20000"
                     /detection_rule="(subtilosin or thuricin or TIGR04404 or
                     Subtilosin_A or skfc)"
                     /neighbourhood="10000"
                     /product="sactipeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      367..1989
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(subtilosin or thuricin or TIGR04404 or
                     Subtilosin_A or skfc)"
                     /neighbourhood="10000"
                     /product="sactipeptide"
                     /protocluster_number="1"
     cand_cluster    1..7358
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(subtilosin or thuricin or TIGR04404 or
                     Subtilosin_A or skfc)"
                     /kind="single"
                     /product="sactipeptide"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..7358
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000602"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..7358
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="sactipeptide"
                     /region_number="1"
                     /rules="(subtilosin or thuricin or TIGR04404 or
                     Subtilosin_A or skfc)"
                     /subregion_numbers="1"
                     /tool="antismash"
     sig_peptide     367..390
                     /gene="sbo"
     gene            367..498
                     /gene="sbo"
     CDS             367..498
                     /codon_start=1
                     /db_xref="GOA:O07623"
                     /db_xref="PDB:1PXQ"
                     /db_xref="UniProtKB/Swiss-Prot:O07623"
                     /function="cyclic peptide antibiotic"
                     /gene="sbo"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     sactipeptide: Subtilosin_A"
                     /gene_functions="biosynthetic-additional (sactipeptides)
                     predicted sactipeptide"
                     /gene_kind="biosynthetic"
                     /product="subtilosin A"
                     /protein_id="CAD23198.1"
                     /sec_met_domain="Subtilosin_A (E-value: 1.7e-31, bitscore:
                     97.4, seeds: 2, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKAVIVENKGCATCSIGAACLVDGPIPDFEIAGATGLFGLWG"
     CDS_motif       367..396
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence: MKKAVIVENK"
                     /locus_tag="sbo_sactipeptide"
                     /aSTool="sactipeptides"
                     /tool="antismash"
     CDS_motif       397..498
                     /prepeptide="core"
                     /locus_tag="sbo_sactipeptide"
                     /peptide="sactipeptide"
                     /predicted_class
                     /score="32.00"
                     /molecular_weight="0.0"
                     /monoisotopic_mass="0.0"
                     /core_sequence="GCATCSIGAACLVDGPIPDFEIAGATGLFGLWG"
                     /aSTool="sactipeptides"
                     /tool="antismash"
                     /leader_sequence="MKKAVIVENK"
                     /leader_location="[366:396](+)"
     mat_peptide     391..495
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /gene="sbo"
                     /product="subtilosin A"
     gene            643..1989
                     /gene="ywiA"
     CDS             643..1989
                     /codon_start=1
                     /db_xref="GOA:P71011"
                     /db_xref="UniProtKB/Swiss-Prot:P71011"
                     /function="putative role in subtilosin biosynthesis"
                     /gene="ywiA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     sactipeptide: subtilosin"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Subtilosin_rSAM_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR04085"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) SPASM"
                     /gene_kind="biosynthetic"
                     /product="ywiA protein"
                     /protein_id="CAD23199.1"
                     /sec_met_domain="subtilosin (E-value: 0.0, bitscore:
                     1078.3, seeds: 6, tool: rule-based-clusters)"
                     /sec_met_domain="Subtilosin_rSAM_RRE (E-value: 1.9e-32,
                     bitscore: 100.8, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 1.5e-09, bitscore: 27.2,
                     seeds: 461, tool: rule-based-clusters)"
                     /sec_met_domain="PF04055 (E-value: 1.4e-24, bitscore: 76.5,
                     seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR04085 (E-value: 5.1e-13, bitscore:
                     38.5, seeds: 281, tool: rule-based-clusters)"
                     /sec_met_domain="SPASM (E-value: 2.6e-12, bitscore: 36.3,
                     seeds: 166, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFIEQMFPFINESVRVHQLPEGGVLEIDYLRDNVSISDFEYLDLN
                     KTAYELCMRMDGQKTAEQILAEQCAVYDESPEDHKDWYYDMLNMLLNKQVVQLENQSTR
                     HAINTSGSNEFPMPLHATFELTHRCNLKCAHCYLESSPEALGTVSIEQFKKTADMLFDN
                     GVLTCEITGGEIFVHPNANEILDYVCKKFKKVAVLTNGTLMRKESLELLKAYKQKIIVG
                     ISLDSVNSEIHDSFRGRKGSFAQTCKTIKLLSDHGIFVRVAMSVFEKNMWEIHDMAQKV
                     RDLGAKAFSYNWVDDFGRGRDIVHPTKNAEQHRKFMEYEQNIIDEFKDLIPIIPYERKR
                     AANCGAGWKSIVISPFGEVRPCALFPKEFSLGNIFHDSYESIFNSPLVHKLWQAQAPRF
                     SEHCMKDKCPFSSYCGGCYLKGLNSNKYHRKNICSWAKNEQLEDVVQLI"
     gene            2002..2163
                     /gene="ywhR"
     CDS             2002..2163
                     /codon_start=1
                     /db_xref="GOA:P71010"
                     /db_xref="UniProtKB/Swiss-Prot:P71010"
                     /gene="ywhR"
                     /product="ywhR protein"
                     /protein_id="CAD23200.1"
                     /transl_table=11
                     /translation="MSPAQRRILLYILSFIFVIGAVVYFVKSDYLFTLIFIAIAILFGM
                     RARKADSR"
     gene            2160..2879
                     /gene="ywhQ"
     CDS             2160..2879
                     /codon_start=1
                     /db_xref="GOA:P71009"
                     /db_xref="UniProtKB/Swiss-Prot:P71009"
                     /gene="ywhQ"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 126.4; E-value:
                     2.4e-38)"
                     /gene_kind="transport"
                     /product="ywhQ protein"
                     /protein_id="CAD23201.1"
                     /transl_table=11
                     /translation="MSILDIHDVSVWYERDNVILEHVDLHLEKGAVYGLLGVNGAGKTT
                     LINTLTGVNRNYSGGFTLCGIEAEAGMPQKTSDQLKIHRYFAADYPLLFTEITAKDYVS
                     FVHSLYQKDFSERQFASLAEAFHFSKYINRRISELSLGNRQKVVLMTGLLLRAPLFILD
                     EPLVGLDVESIEVFYQKMREYCEEGGTILFSSHLLDVVQRFCDFAAILHNKQIQKVIPI
                     GEETDLRREFFEVIGHE"
     gene            2824..4182
                     /gene="ywhP"
     CDS             2824..4182
                     /codon_start=1
                     /db_xref="GOA:Q8RKH4"
                     /db_xref="UniProtKB/Swiss-Prot:Q8RKH4"
                     /gene="ywhP"
                     /product="ywhP protein"
                     /protein_id="CAD23202.1"
                     /transl_table=11
                     /translation="MGRRPICGGNFLRLSAMNNIFPIMSLLFKQLYSRQGKKDAIRIAA
                     GLVILAVFEIGLIRQAGIDESVLGKTYIILALLLMNTYMVFLSVTSQWKESYMKLSCLL
                     PISSRSFWLAQSVVLFVDTCLRRTLFFFILPLFLFGNGTLSGAQTLFWLGRFSFFTVYS
                     ILFGVMLSNHFVKKKNSMFLLHAAVFAFVCLSAAFMPAVTIPLCAVHMLWAVIIDFPVF
                     LQAPPHQSKMHFFMRRSEFSFYKREWNRFISSKAMLLNYVVMAAFSGFFSFQMMNTGIF
                     NQQVIYIVISALLLICSPIALLYSIEKNDRMLLITLPIKRRTMFWAKYRFYSGLLAGGF
                     LLVAIIVGFISGRPISALTFVQCMELLLAGAFIRLTADEKRPSFGWQTEQQLWSGFSKY
                     RSYLFCLPLFLATLAGTAVSLAVIPIAALIIVYYLQKQDGGFFDTSKRERIGS"
     gene            4172..5332
                     /gene="ywhO"
     CDS             4172..5332
                     /codon_start=1
                     /db_xref="GOA:Q8RKH3"
                     /db_xref="InterPro:IPR007863"
                     /db_xref="UniProtKB/Swiss-Prot:Q8RKH3"
                     /gene="ywhO"
                     /product="ywhO protein"
                     /protein_id="CAD23203.1"
                     /transl_table=11
                     /translation="MAVNLLKTQQFSTISIAASFLKPIESAAEPEEETIYFYSAAAHLK
                     QQIIDAFGYAAGCRFMYSANLFFDQQLKTCGTRLIHPSFNKNLHLDALMKTFADMSFPS
                     SLSADAVEKAKDELLLKIEKKFADPFSYSAARLAEEAFGNPMYGTAMFGRKDRIQAIHP
                     QRFLNATDFIVDLLSQHKQLNILGHVQACDIPGHASQTSAVTAGRFLVNRHVFETETRS
                     AAGPSVLTLGFDCGEMKDASDYIKIQLIDGLLGKYGHSALFKHFREKDLAVYHVITRYD
                     IMNNLLLVSICTNQLHEKEIPPRVLEAVSHFSADERELEQAKQFFRNEMLLQFDSPEGL
                     LAYMGILRRFSCTKEDLLDGISAVTCRDVLQFITTINYIGAHVVRG"
     gene            5337..6620
                     /gene="ywhN"
     CDS             5337..6620
                     /codon_start=1
                     /db_xref="GOA:Q8RKH2"
                     /db_xref="InterPro:IPR001431"
                     /db_xref="InterPro:IPR011765"
                     /db_xref="UniProtKB/Swiss-Prot:Q8RKH2"
                     /gene="ywhN"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_M16"
                     /gene_kind="biosynthetic-additional"
                     /product="ywhN protein"
                     /protein_id="CAD23204.1"
                     /sec_met_domain="Peptidase_M16 (E-value: 1.8e-16, bitscore:
                     49.6, seeds: 24, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEKKAFFQQLDERTDIRYTDSGMKIIRLKFPRAHLRLCNVKIDFG
                     SRDVCLQAESGDTLLPYGTAHFLEHLLFWHNGRNLYTDFFAHGALLNAFTTYTDTNFMF
                     TSLPDRLRQTIPILLDALWNHSFDKKMITQEKAVITSEIQTAHLNHQLYYHYQLISMLS
                     PASPAAVFPAGRIEDIEALDIRDLQKAYNAAYQPQRMTLFLIGGSEDTEALLPPHLRLE
                     KRPNHKAERKIISACPPPALSQKMVLGNEERIEDTWTGLQVGAIPGQNNLLTLKLYWDI
                     ASRILFQLDSPFFQEIQQTYRLEIDCLSAEAHMYEDGGFLILHSQGAHSSAYIDVASYY
                     VTQQKQQIETWLQYGKDSLTDAIIYDSDYVRKCFEWAAECDRCDCTFLDMYRIIHDMNS
                     QDFLSLIDALASSKKAVIHVSQKEAIGQ"
     gene            6617..7318
                     /gene="ywhM"
     CDS             6617..7318
                     /codon_start=1
                     /db_xref="GOA:Q8RKH1"
                     /db_xref="UniProtKB/Swiss-Prot:Q8RKH1"
                     /gene="ywhM"
                     /product="ywhM protein"
                     /protein_id="CAD23205.1"
                     /transl_table=11
                     /translation="MSKSTVFTVLLLLLGMAAYSFGWVQAVAEAAAQYVQIFNNDALRL
                     GLLACIAAVLMLPAFLYLHYVTQSVKNMTAAFQKLTQSHQSCCDFQHHRLCSRYAEEVK
                     SLRDRYKNVRQTYVMAAVLCQVIIFGCMFEIVKAVPFRLHTPPIVSTGMALLLILYLLF
                     YMRMYLLKLFQHGTLFKKILACVLTGAGIGWMLSFTISELLFLIILAAIQQIGSFIYKR
                     FSNRGFTSLDL"
ORIGIN
        1 tcatcctcga tcacagactt cacatggagt gttatcggtg tctcttgctt cattttgaca
       61 cgtccttttc atcattccac tttgacttga atagtataat cattccgttt gtaaacttca
      121 actgcttcta tcttaccatc attgctcatc agatttgaag ataaacctca taaaaagcat
      181 ttccttaata gaagagaaaa tgatatcact aattaccttt aggaaatgtt acatttttcc
      241 gaaatctatc attttcattt cacattttct tcaaaatata cgtattgaat tggtaattta
      301 atagttttat cataaaagta caacatagat ctgctagaaa aaaacaaaaa aagggaggat
      361 tcaattatga aaaaagctgt cattgtggaa aacaaaggtt gtgcaacatg ctcgatcgga
      421 gccgcttgtc tagtggacgg tcctatccct gattttgaaa ttgccggtgc tacaggtcta
      481 ttcggtctat ggggataaga ttctccaaaa gggcatagtc attctgctat gcccttttta
      541 gtaaattaca atttttaact aaaatatacc tcttggatct acctcttggt tatacataca
      601 caatcgtttt tcatttgaat catatgtata ggggatggta ttttgtttat agagcagatg
      661 tttccattta ttaatgaaag tgtaagagtt caccagcttc ctgagggcgg cgtgttagaa
      721 atcgactact tgcgcgataa tgtctccatt tctgactttg agtatttgga tcttaacaaa
      781 acggcttacg agctctgcat gcgaatggat gggcaaaaaa cggctgaaca gattttagcg
      841 gaacaatgcg ccgtgtacga tgaatcaccg gaagaccata aggattggta ttacgatatg
      901 ctcaacatgc tcctgaacaa gcaggtcgtc cagcttgaaa atcagtccac ccgccacgcg
      961 atcaacacga gcggaagcaa tgaatttccg atgccgctgc acgccacctt tgagctgacg
     1021 caccgctgta atttgaaatg cgcccactgt tatttggaaa gttcgccgga agcgctcggc
     1081 accgtgtcga ttgagcaatt caagaaaacg gctgatatgc tgtttgacaa cggtgtattg
     1141 acatgcgaaa tcacgggcgg agaaattttt gtccatccga acgccaatga gattcttgac
     1201 tatgtgtgta aaaagttcaa aaaggtcgct gtcttaacga acggaacact catgcgaaaa
     1261 gagagcctgg agcttctgaa agcttacaaa caaaaaatca tcgtcggcat ttctctcgat
     1321 agtgtcaatt ccgagatcca tgactccttt agagggagaa aaggctcttt tgcccaaact
     1381 tgtaaaacga taaaattgtt gagtgaccac ggtatatttg tcagagtcgc catgtccgtt
     1441 ttcgaaaaaa acatgtggga aatccacgat atggcccaaa aggttcggga tctcggggca
     1501 aaggcgttct cttacaattg ggttgatgat ttcggaagag gcagagatat tgtccacccc
     1561 acaaaaaatg ccgagcagca ccgcaaattt atggagtacg agcaaaatat cattgatgag
     1621 tttaaagatc tgattccgat tattccctat gagagaaaac gcgcggcaaa ttgcggcgct
     1681 ggctggaagt ccattgtgat cagcccgttc ggggaagtac gtccgtgtgc gctctttcca
     1741 aaggaatttt cattgggaaa tatttttcat gattcatatg aaagcatctt taactcccct
     1801 ctcgtccata aactgtggca agcgcaggcg ccgcggttca gcgagcattg catgaaagac
     1861 aaatgcccgt tcagcagcta ttgcggaggc tgttacttaa aagggctgaa ctctaacaaa
     1921 tatcaccgaa aaaacatttg ctcttgggca aaaaatgaac aattagaaga cgtggtccag
     1981 cttatttagg agggaaacga tttgtcacca gcacaaagaa gaattttact gtatatcctt
     2041 tcatttatct ttgtcatcgg cgcagtcgtc tattttgtca aaagcgatta tctatttacg
     2101 ctgattttca tagccattgc cattcttttc gggatgcgcg cgcggaaggc tgactcgcga
     2161 tgagcatttt ggatatacac gatgtatccg tttggtatga acgggacaac gtcatcttag
     2221 agcacgtgga cttacactta gaaaaaggcg ccgtttacgg attgcttggg gtaaacggtg
     2281 ccggcaaaac aacactgatc aatacgctga caggagtgaa ccgcaattac agcgggggct
     2341 ttacgctgtg cggcattgaa gctgaggccg gcatgccgca gaaaacatca gatcaactga
     2401 agattcaccg ttacttcgcc gctgattatc cgctgctgtt tacagaaatt acggcgaagg
     2461 actatgtgtc tttcgtccat tcgctttatc aaaaggattt ttcagagcga cagtttgcca
     2521 gtttggctga ggcctttcat ttttcaaaat acatcaacag gagaatctcg gagctgtcct
     2581 tggggaacag gcaaaaggtt gtgttgatga caggattatt gctgcgggct cccctgttta
     2641 ttttggatga gccgctcgtc ggtttggatg tggaatcaat agaggtcttt tatcagaaaa
     2701 tgcgggagta ctgtgaggaa ggcggaacca ttttgttttc ttcccatctg ctcgatgtcg
     2761 tgcagagatt ttgtgatttt gcggccattc tgcacaacaa acagatccaa aaggtcattc
     2821 cgattgggga ggagaccgat ctgcggcggg aattttttga ggttatcggc catgaataac
     2881 atattcccca tcatgtcgtt gctgttcaaa cagctgtaca gccggcaagg gaaaaaggac
     2941 gctatccgca ttgctgcagg gcttgtgatt ctcgccgtgt ttgaaatcgg gctgatccga
     3001 caagccggca ttgacgaatc ggtgttggga aaaacgtata tcatattggc gcttctctta
     3061 atgaacacgt atatggtgtt tctttccgtg acatcacaat ggaaggaatc ttatatgaag
     3121 ctgagctgtc tgctgccgat ttcatcacgg agcttttggc tcgcccagag tgtcgttctg
     3181 tttgtcgata cctgtttgag aagaacgtta ttctttttta ttttaccgct gttcttattt
     3241 ggaaacggaa cgctgtcagg ggcgcaaaca ttgttttggc ttggcagatt ttcgtttttt
     3301 accgtttact cgattctatt cggagttatg ctaagcaacc atttcgtcaa aaagaagaac
     3361 tcgatgtttc tgctgcatgc ggcggtattc gcctttgtat gcctcagtgc cgcttttatg
     3421 ccggccgtca cgatcccgct atgcgcggtt cacatgctat gggcggtgat cattgacttt
     3481 ccggtctttc tgcaggcgcc tccgcatcag agcaagatgc atttttttat gcggcgatct
     3541 gaattttcgt tttacaaaag agaatggaac cgatttattt cttctaaagc gatgctgtta
     3601 aattacgtgg tgatggcggc gttcagcgga ttcttttcgt tccagatgat gaacactggc
     3661 atcttcaatc agcaagtgat ttatattgtg atttccgctc tattgctgat ttgctcgccg
     3721 atcgcccttt tgtactctat tgaaaaaaac gatcgcatgc tgctcatcac gcttccaatt
     3781 aaaagaagaa cgatgttttg ggcgaaatat cgcttttatt caggcctatt ggcaggcgga
     3841 tttctccttg tcgctatcat tgtgggcttc atcagcggcc gaccgatttc agcccttact
     3901 tttgtgcagt gtatggaatt gctgctggcg ggagcgttta tccggttaac cgcagatgaa
     3961 aaaaggccgt ccttcggctg gcagacagag cagcagctct ggagcggatt ttcaaaatac
     4021 cgatcttatt tattttgtct tccccttttc ttagccacat tagccggcac cgctgtctcc
     4081 ttggcggtga ttccaattgc cgcgctgatc atagtctatt acctgcaaaa acaggatggt
     4141 ggtttctttg atacaagcaa aagagaacgg attggcagtt aacctgctga agactcaaca
     4201 attttcaaca atctcaatcg ccgcgagctt tctaaaaccg atcgaaagcg cggcggagcc
     4261 tgaagaagaa acaatctatt tttacagcgc ggctgcacat ctgaagcagc aaatcattga
     4321 cgctttcggt tatgccgccg gctgccgctt tatgtattca gcgaaccttt tttttgacca
     4381 gcagctgaaa acctgcggaa cccgtttgat tcacccatca tttaacaaga atcttcatct
     4441 tgatgccctg atgaagacat ttgcagacat gtccttccct tcttctcttt cagccgatgc
     4501 tgttgaaaag gcaaaggatg aactgctgct gaaaatcgaa aagaaattcg ctgacccctt
     4561 ctcctactcg gcagcccgct tggcggagga ggccttcgga aacccgatgt acggcacagc
     4621 catgtttggc cgaaaagaca ggatacaggc aatccatcca caacggtttc taaatgcaac
     4681 ggatttcatc gtagatttat tgtcccagca taaacagctg aacatattgg gacatgtaca
     4741 agcatgtgat attccgggac atgcctcgca aacatccgcc gtgacggccg gaagattcct
     4801 cgtgaaccgg catgtttttg aaacagagac acgcagcgca gcaggtcctt ccgtcttgac
     4861 actcggattt gactgcgggg agatgaaaga tgccagcgat tatatcaaaa tccagctgat
     4921 agacggattg ctcggaaagt acggacattc cgcattattc aagcacttcc gcgaaaagga
     4981 tctggcagtc tatcatgtca tcacccgata cgacatcatg aacaatttgc tgcttgtttc
     5041 aatatgtacg aatcagcttc acgagaaaga gattcccccg cgcgttttgg aagctgtctc
     5101 acatttttca gccgatgagc gggagctaga acaggcaaag caatttttcc gcaatgaaat
     5161 gctgcttcaa ttcgattctc ccgaagggct tttagcctat atgggaatcc tgcgccgctt
     5221 ctcctgcacg aaagaagact tgctggatgg catctcagcg gttacgtgcc gcgatgtatt
     5281 gcaattcata acgactatta attatatcgg agctcatgta gtaaggggat gaagagatgg
     5341 agaagaaagc cttttttcaa cagctggatg aacgaaccga tattagatac acagacagcg
     5401 ggatgaaaat aatcagattg aaattccccc gcgcccattt gcggctgtgc aatgtgaaaa
     5461 tcgattttgg aagccgggat gtctgcttac aggcagaatc aggcgacacg cttctcccgt
     5521 atggcacagc gcatttttta gagcatcttc tcttctggca taatggccgc aatctgtaca
     5581 ctgatttttt tgcgcatggc gcgcttttaa acgcgttcac cacctacacc gataccaatt
     5641 tcatgtttac ctcattgccg gatcgactca gacagacgat ccccatcctg cttgatgcgt
     5701 tatggaacca ctcatttgac aagaagatga taactcagga aaaagctgtg attacaagcg
     5761 aaattcaaac agcacacctc aatcatcagc tctattacca ttatcagctg atcagcatgc
     5821 tttctcccgc ctcacctgcc gccgtcttcc ccgcaggcag aatcgaggac attgaggcgc
     5881 tggacatccg tgatttacaa aaagcctaca atgccgccta tcagcctcag cggatgacgc
     5941 tgtttttgat cggaggctca gaagacacgg aagccctttt gccccctcac ctccggctgg
     6001 aaaagcgtcc aaatcataaa gcagaaagaa aaatcatctc cgcatgccct ccaccagccc
     6061 tctcacaaaa aatggtgctg ggtaatgagg agcggattga agacacatgg accggattac
     6121 aggttggcgc aattcccggg caaaacaatc tgttgacatt aaagctttat tgggacatcg
     6181 catctcgcat tctatttcaa ttagattcac cattttttca agagatacag caaacatacc
     6241 gcctggaaat cgattgcttg tcagccgagg ctcacatgta tgaggatggc ggttttttga
     6301 ttcttcacag tcaaggggcg cattcttcgg cttatattga tgttgcttcc tactatgtca
     6361 cacagcaaaa acaacagatt gagacttggc tccaatacgg caaggattct ttgacggacg
     6421 cgatcattta tgacagcgat tatgttcgca aatgcttcga atgggcggcc gagtgtgaca
     6481 ggtgcgattg cacatttctc gacatgtacc gaatcattca cgatatgaat agccaagatt
     6541 tcttgtctct cattgatgca ttggcatctt ctaaaaaagc ggtcattcac gtttctcaga
     6601 aagaggcgat cggacaatga gcaaaagcac tgtctttacc gtgttgttgt tgctcttagg
     6661 tatggcggct tattccttcg gctgggttca agccgtcgcc gaagccgcag cgcaatatgt
     6721 acagatattc aataacgatg ctttgcggct cggactgctg gcttgtatcg ctgccgttct
     6781 gatgctgcct gcttttctct atcttcacta tgtcacccaa tccgtaaaaa acatgacagc
     6841 agcttttcag aaactcacac agtctcatca aagctgctgt gatttccagc atcacagatt
     6901 atgcagccgt tatgcagaag aggtcaaatc gctgcgtgac aggtataaaa acgtccgtca
     6961 aacatacgtg atggctgctg tattatgcca agtcatcata ttcggctgca tgttcgaaat
     7021 cgttaaagcc gtccctttcc gccttcacac gcctcctatc gtttcaacgg gaatggcatt
     7081 gttactgatt ttgtatttat tgttttacat gcgaatgtac ttgctgaagc tttttcaaca
     7141 tggcaccctg ttcaaaaaaa ttcttgcatg cgtgctgaca ggagccggga ttgggtggat
     7201 gctcagtttc accataagtg aactgctgtt tctgatcatc ctggctgcca tccagcagat
     7261 cggatcattt atttataaac gattctctaa tcgcggcttc acttctctag acctatgaaa
     7321 tagctacatc tcaatcgcag acggacccgt gtcgaccg
//
