LOCUS       BGC0000608              4495 bp    DNA     linear   BCT 02-JUN-2011
DEFINITION  Amycolatopsis fastidiosa strain ATCC 202099 nocathiacin biosynthetic
            gene cluster, partial sequence.
ACCESSION   BGC0000608
VERSION     BGC0000608
KEYWORDS    .
SOURCE      Actinokineospora fastidiosa
  ORGANISM  Actinokineospora fastidiosa
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Actinokineospora.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 13:55:20
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..4495
                     /culture_collection="ATCC:202099"
                     /db_xref="taxon:1816"
                     /mol_type="genomic DNA"
                     /organism="Actinokineospora fastidiosa"
                     /strain="ATCC 202099"
     misc_feature    <1..>4495
                     /note="nocathiacin biosynthetic gene cluster"
     protocluster    1..4495
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[304:4371]"
                     /cutoff="20000"
                     /detection_rule="((YcaO or TIGR03604) and ((thio_amide and
                     (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or
                     Lant_dehydr_N or PF07366 or PF06968 or PF04055) or
                     thiostrepton)"
                     /neighbourhood="10000"
                     /product="thiopeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      305..4371
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="((YcaO or TIGR03604) and ((thio_amide and
                     (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or
                     Lant_dehydr_N or PF07366 or PF06968 or PF04055) or
                     thiostrepton)"
                     /neighbourhood="10000"
                     /product="thiopeptide"
                     /protocluster_number="1"
     cand_cluster    1..4495
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((YcaO or TIGR03604) and ((thio_amide and
                     (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or
                     Lant_dehydr_N or PF07366 or PF06968 or PF04055) or
                     thiostrepton)"
                     /kind="single"
                     /product="thiopeptide"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..4495
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000608"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..4495
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="thiopeptide"
                     /region_number="1"
                     /rules="((YcaO or TIGR03604) and ((thio_amide and (PF06968
                     or PF04055 or PF07366)) or Lant_dehydr_C or Lant_dehydr_N
                     or PF07366 or PF06968 or PF04055) or thiostrepton)"
                     /subregion_numbers="1"
                     /tool="antismash"
     CDS             complement(305..1498)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     thiopeptide: PF06968"
                     /gene_kind="biosynthetic"
                     /note="radical S-AdoMet:biotin and thiamin
                     synthesis-associated protein"
                     /product="NocE"
                     /protein_id="ADH93698.1"
                     /sec_met_domain="PF06968 (E-value: 2.6e-12, bitscore: 35.0,
                     seeds: 824, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAEYPGTGHPDFVLPDLAQVRIEAATVDTRAVLALGPDDEPGASR
                     PAVALALWEDESVSTAELVAAAELRCAERTPRLHTYVPLYTTNHCDSECKMCSMRKGNA
                     RMERKFSGRDEIIDQLRILFEHEGVRGVGFLTGEYEDKYTRLSTAFRIGWAIRTALDMG
                     FERVYFNIGSMEPDEIDVLAEWVRRDDPVTMCVFQESYDRETYSKFMGGTVSGVSKADY
                     DRRVVSFDRWLDKGFRYVNPGVLIGLHLDVAAELVTLVAHGAHLKDRDAVVDLSVPRMR
                     PAMSSRDSTRIKDDEYMRLMAVLAFTCPEQRLVLTTREPQEFQDQAIGLAGVISPGSPD
                     VAPYRADSQARNEETTSQFLVADLRRPRHILGRIEAGGTKVGHFANPSVGAAVIPIG"
     CDS             1889..2038
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     thiopeptide: thiostrepton"
                     /gene_functions="biosynthetic-additional (thiopeptides)
                     predicted thiopeptide"
                     /gene_kind="biosynthetic"
                     /note="nocathiacin precursor peptide"
                     /product="NocA"
                     /protein_id="ADH93699.1"
                     /sec_met_domain="thiostrepton (E-value: 7.8e-15, bitscore:
                     43.1, seeds: 4, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSADLSALNIDSLEISEFLDDSRLEDSEVVAKVMSASCTTCECSC
                     SCSS"
     CDS_motif       1889..1996
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence:
                     MSADLSALNIDSLEISEFLDDSRLEDSEVVAKVMSA"
                     /locus_tag="ADH93699.1_thiopeptide"
                     /aSTool="thiopeptides"
                     /tool="antismash"
     CDS_motif       1997..2038
                     /prepeptide="core"
                     /locus_tag="ADH93699.1_thiopeptide"
                     /peptide="thiopeptide"
                     /predicted_class="Type I"
                     /score="11.30"
                     /molecular_weight="1300.4"
                     /monoisotopic_mass="1299.4"
                     /core_sequence="SCTTCECSCSCSS"
                     /aSTool="thiopeptides"
                     /tool="antismash"
                     /leader_sequence="MSADLSALNIDSLEISEFLDDSRLEDSEVVAKVMSA"
                     /leader_location="[1888:1996](+)"
                     /alternative_weights="1318.5"
                     /alternative_weights="1336.5"
                     /alternative_weights="1354.5"
                     /alternative_weights="1372.5"
                     /alternative_weights="1390.5"
                     /alternative_weights="1408.6"
                     /alternative_weights="1426.6"
     CDS             2258..3433
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     thiopeptide: PF04055"
                     /gene_functions="other (smcogs) SMCOG1277:Radical SAM
                     domain protein (Score: 62.8; E-value: 4e-19)"
                     /gene_kind="biosynthetic"
                     /note="SAM-dependent oxidase or methyltransferase"
                     /product="NocB"
                     /protein_id="ADH93700.1"
                     /sec_met_domain="PF04055 (E-value: 2.4e-18, bitscore: 55.2,
                     seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLMLYVHVPFCHSKCTFCDWVQAIPTKDLLRKPEDSVRKNYVRAL
                     VTEIETRGAQLRAAGQVPYVVYWGGGTASSLDNAEAEAIWGALDSAFDLSTVAEATIEC
                     SPDTVDKAKLEFFRGLGFNRVSSGVQSFDDARLRRLGRRHTAGEADRIVHHAREAGFDE
                     VSIDIMSGFPDQELDELRATVEKAVSLPLTHLSLYSFRPTPGTFMRRKLAGTEKRAYLR
                     KQQALFTEARRMIIDAGLPEYASGYFGRVSPFAAMYFQLRADTAGFGSGAISLVDRQFL
                     SHAKGKLHAYIQDPLAYDIDVPAGQDRVLVSFLQAGLAMFDGVLREEWRVSTGTDLDEV
                     LTRPSIAPLADFLRGRGLIEDERGIRLDPRLAGLTLIELAFEMAMSQPESA"
     CDS             3430..4371
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     thiopeptide: Lant_dehydr_C"
                     /gene_kind="biosynthetic"
                     /note="hypothetical protein"
                     /product="NocC"
                     /protein_id="ADH93701.1"
                     /sec_met_domain="Lant_dehydr_C (E-value: 1.8e-13, bitscore:
                     38.8, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTWTELVFPATQDEQPGLVAGVLAPLLADLDRPGLFLRELGPEGA
                     TRLLLQVRDAPPDLPTRTAALPVQPTAVRAATVAPLGGPVFDGPGLDETTRGFLADTAP
                     VAVDLSTRPDRTGAALTLMTAHLAAVADPARSGDGPPLSFLSFRSHAEAFLATTRDPNA
                     ARHAFDTRYTDHRTTVKAAVRAILLDGDPGDAAPWSAAARAAKPRFTAGFACADLVAHT
                     GYTRDYLRERTDFADNPFHSRAGASEQLQAYLGGDPSFLATRLLTSLLYVTLHSSGVSL
                     MQRYFLCHAIAKACESIYHVDSMSLLADLAVG"
ORIGIN
        1 gccttaaggc ctaggagacg ccaaggtggg aggccgtgac caggtggccg gggccgaagt
       61 ggtccaccag ggccgcgacg tactccagtg cggtgtccag gtagtccggc ccggcgtcga
      121 ccgggcacca gccgtggccg ggcaggtcga ccggcaccac ccggcgccgc cccgaccacg
      181 cggcgatctg cacgccgaag tggccgtagg cggtgccgag caggccgtgc aggaagtaca
      241 ccggggaact gccgtccccg gccgcctcgc gcgacatcgt gattcctccg ggagtcgtcc
      301 tgcctcagcc gatcgggatg acggccgcgc ccaccgacgg gttggcgaag tggccgacct
      361 tggtgccgcc cgcctcgatg cggcccagga tgtgtcgggg cctgcgcagg tcggcgacca
      421 ggaactgcga ggtcgtctcc tcgttgcggg cctgcgagtc ggcgcggtag ggcgccacgt
      481 ccgggctgcc cgggctgatg acgcccgcca ggccgatcgc ctggtcctgg aactcctgcg
      541 gctcgcgggt ggtcagcacc aggcgctgct cggggcaggt gaaggcgagc acggccatga
      601 gccgcatgta ctcgtcgtcc ttgatccggg tgctgtcccg cgagctcatg gccgggcgca
      661 tgcgcggcac cgacaggtcc acgacggcgt cgcggtcctt caggtgcgcc ccgtgcgcga
      721 ccagggtgac cagctcggcg gccacatcca ggtgcagtcc gatgaggaca ccggggttga
      781 cgtagcggaa ccccttgtcg agccagcggt cgaaggacac gacgcggcgg tcgtagtcgg
      841 ccttggacac gccgctgacc gtgccgccca tgaacttgct gtacgtctcg cggtcgtagc
      901 tctcctggaa cacgcacatg gtgaccgggt cgtcgcggcg gacccactcg gcgagcacgt
      961 cgatctcgtc gggctccatg ctgccgatgt tgaagtagac ccgctcgaag cccatgtcca
     1021 gcgcggtgcg gatcgcccag ccgatgcgga aggcggtcga gagccgggtg tacttgtcct
     1081 cgtactcgcc ggtgaggaag ccgacgccgc gcacgccctc gtgctcgaac aggatgcgca
     1141 gctggtcgat gatctcgtcg cggccggaga acttgcgctc catgcgggcg ttgcccttgc
     1201 gcatcgagca catcttgcac tcggagtcgc agtggttggt cgtgtagagc gggacgtacg
     1261 tgtgcagccg gggcgtgcgc tccgcgcagc gcagctccgc ggcggcgacc agctccgcgg
     1321 tgctcaccga ctcgtcctcc cagagcgcca gcgcgaccgc gggccgggac gcgcccggct
     1381 cgtcgtccgg gcccagcgcg agcaccgcac gggtgtcgac ggtcgccgcc tcgatgcgca
     1441 cctgagccag atccggcagc acgaagtcgg gatgaccggt accggggtat tccgccatga
     1501 ctagttcctt cccgcgttgc gcggcagatc cgcacaaatg ggttcgctgc ttgattcgac
     1561 tatcggggcc agatatcgcc gaaccggccg cgccagtggc ccagcacgtt cgaggtaaca
     1621 cgaccccggc aggccgtgtc atggggaagt tcctgcaact aggccgctga ccagggaatt
     1681 tacttgtgcc agtagctcag attcatggcg cgtgagccga atcgatggcg atcgtagagc
     1741 ggttatatag cgttctttcg tcgccaccga gaatcttggc agcaagttcg gagactgctt
     1801 gacaccccgg tcgccggatc aataacttcg gtgtcggggc caggcgaatc cggtacgaac
     1861 cacaagccca taagaaagag gtgtttccat gtccgctgac ctgtctgcgc tgaacatcga
     1921 ctccctggag atctcggagt tcctggacga cagccgcctg gaggacagcg aggtcgtggc
     1981 caaggtcatg tcggcctcct gcaccacctg cgagtgctcc tgcagctgct cgtcctgatc
     2041 agcccgaggg gtttcccccc tcgcgtgcgg tgacgtgcgg tcccgcgacc ttcccggccc
     2101 agcctggcca ccggagggtt gcgggaccgc cgcccgcggt caggcgcgga ccacgagaaa
     2161 ggaccgaccc atgtcgaccg ccgtgtctct atcgtccctt gtggacgtgg tgcccgcgcc
     2221 agcgccgaac ctgccgtcct cgaccgacga gcagcacatg ctgatgctct acgtgcacgt
     2281 cccgttctgc cactccaagt gcaccttctg cgactgggtg caggcgatcc cgaccaagga
     2341 cctgctgcgc aagcccgagg actcggtccg caagaactac gtccgcgccc tggtcaccga
     2401 gatcgagacc cggggcgcgc agcttcgggc cgctggccag gtgccctacg tcgtctactg
     2461 gggcggcggc accgcgtcca gcctggacaa cgccgaggcc gaggcgatct ggggcgcgct
     2521 ggactccgcg ttcgacctca gcaccgtcgc cgaggcgacc atcgagtgca gccccgacac
     2581 cgtcgacaag gccaagctgg agttcttccg cggcctgggc ttcaaccgcg tctccagcgg
     2641 cgtgcagtcc ttcgacgacg cgcgcctgcg caggctcggc aggcgccaca ccgcgggcga
     2701 ggccgaccgc atcgtgcacc acgcccgcga ggcgggcttc gacgaggtgt ccatcgacat
     2761 catgtccggc ttccccgacc aggagctgga cgagctgcgc gcgaccgtcg agaaggcggt
     2821 gtcgctgccg ctgacccacc tgtcgctgta ctccttccgc ccgacgccgg gcacgttcat
     2881 gcgccgcaag ctcgcgggca ccgagaagcg cgcgtacctg cgcaagcagc aggccctgtt
     2941 caccgaggcc cgccgcatga tcatcgacgc cggcctgccc gagtacgcct ccggctactt
     3001 cggcagggtg tcgccgttcg cggccatgta cttccagctg cgcgccgaca ccgcgggctt
     3061 cggctccggc gcgatctccc tggtcgaccg ccagttcctc tcccacgcca agggcaagct
     3121 gcacgcctac atccaggacc cgctggccta cgacatcgat gtgcccgcag gccaagaccg
     3181 cgtgctggtc tcgttcctgc aggcaggcct cgccatgttc gacggcgtcc tgcgcgagga
     3241 gtggcgcgtc tccaccggca ccgacctcga cgaggtgctc acccgcccgt cgatcgcccc
     3301 gctcgcggac ttcctgcgcg ggcgcggcct gatcgaggac gagcgcggca tccgcctcga
     3361 cccccggctg gccgggctga ccctgatcga gctggccttc gagatggcca tgtcccagcc
     3421 ggagtcggcg tgacctggac ggaactggtc ttcccggcga cccaggacga gcagcccggc
     3481 ctggtcgccg gggtgctcgc cccactgctg gccgacctcg accgccccgg cctgttcctg
     3541 cgcgaactcg gccccgaggg cgccacccga ctgctgctcc aagtccgcga cgccccaccg
     3601 gacctgccga ccaggaccgc ggccctgccc gtgcagccga cggccgtgcg cgctgccacc
     3661 gtcgccccgc tgggcggccc ggtcttcgac ggccccggcc tggacgagac cacccgcggc
     3721 ttcctggcgg acaccgcgcc ggtggccgtc gacctgtcca cccgccccga ccgcaccggc
     3781 gcggccctga ccctgatgac cgcccacctc gccgcggtgg ccgacccggc ccgctccggc
     3841 gacggccctc cgctgagctt cctcagcttc cgctcccacg ccgaggcgtt cctggccacc
     3901 acccgcgacc cgaacgccgc ccggcacgcc ttcgacaccc gctacaccga ccaccgcacc
     3961 accgtcaaag ccgccgtccg ggcgatcctg ctcgacggcg accctggcga cgccgcgccc
     4021 tggtccgccg cggcccgcgc cgcgaaaccc cggttcaccg cgggcttcgc ctgcgcggac
     4081 ctggtcgccc acaccggcta cacccgcgac tacctccggg aacgcacgga cttcgcggac
     4141 aacccgttcc acagcagggc gggcgcgtcc gagcagctac aggcgtacct gggcggcgac
     4201 ccgtcattcc tggcgacccg cctgctcacc agcctgctct acgtgaccct gcactcgtcg
     4261 ggggtcagcc tgatgcagcg ctacttcctc tgccacgcca tagccaaggc ctgcgagtcg
     4321 atctaccacg tcgacagcat gagcctgctg gcagacctgg cggtcgggta gcccggactc
     4381 ccgcggtgcc gctcccgtca gcgggggcgg cgccgcggtg tcagatcatc cgtcgcgcat
     4441 caccggccgc ttgctgctca gcgcaaggtc gtgagcaggt ctcggcaggc ccgcg
//
