BGC0000608 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000608 (Actinokineospora fastidiosa)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	thiopeptide	1	4,495	nocathiacin I	ribosomal:RiPP:Thiopeptide	100%

BGC0000608 - Region 1 - thiopeptide

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Location: 1 - 4,495 nt. (total: 4,495 nt) Show pHMM detection rules used

Region on contig edge.

thiopeptide: ((YcaO or TIGR03604) and ((thio_amide and (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or Lant_dehydr_N or PF07366 or PF06968 or PF04055) or thiostrepton)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

MIBiG comparison

KnownClusterBlast

Thiopeptides

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 4,496
Label		BGC0000608

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000608	1.00	RiPP	nocathiacin	Actinokineospora fastidiosa
BGC0001707	0.23	RiPP	nosiheptide	Streptomyces sp.
BGC0000610	0.20	RiPP	nosiheptide	Streptomyces actuosus
BGC0000609	0.18	RiPP	nocathiacin	Nocardia sp. ATCC 202099
BGC0000613	0.11	RiPP	thiomuracin A	Nonomuraea sp. Bp3714-39
BGC0001860	0.10	RiPP	thiomuracin	Thermobispora bispora DSM 43833
BGC0001155	0.10	RiPP	GE2270	Planobispora rosea
BGC0000604	0.10	RiPP	GE2270A	Nonomuraea sp. WU8817
BGC0000809	0.05	Alkaloid	AT2433-A1	Actinomadura melliaura
BGC0001020	0.04	NRP, Polyketide	muraymycin C1	Streptomyces sp. NRRL 30471

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000608	1.00	RiPP	nocathiacin	Actinokineospora fastidiosa
BGC0001707	0.56	RiPP	nosiheptide	Streptomyces sp.
BGC0000610	0.52	RiPP	nosiheptide	Streptomyces actuosus
BGC0000609	0.50	RiPP	nocathiacin	Nocardia sp. ATCC 202099
BGC0000613	0.37	RiPP	thiomuracin A	Nonomuraea sp. Bp3714-39
BGC0001860	0.37	RiPP	thiomuracin	Thermobispora bispora DSM 43833
BGC0001155	0.36	RiPP	GE2270	Planobispora rosea
BGC0000604	0.36	RiPP	GE2270A	Nonomuraea sp. WU8817
BGC0000809	0.21	Alkaloid	AT2433-A1	Actinomadura melliaura
BGC0001020	0.17	NRP, Polyketide	muraymycin C1	Streptomyces sp. NRRL 30471

Similar known gene clusters from MIBiG 4.0

Thiopeptide predictions

Thiopeptide(s)

ADH93699.1

Leader:

MSADLSALNIDSLEISEFLDDSRLEDSEVVAKVMSA

Core:

SCTTCECSCSCSS

Core with dehydrations:

DhaCysDhbDhbCysECysDhaCysDhaCysDhaDha

antiSMASH-DB 4.0 matches for ADH93699.1

100.0% NZ_BMRB01000004.1and 4 others:

NZ_CP031087.1 Thiopeptides: Actkin_RS12050_thiopeptideNZ_CP046172.1 Thiopeptides: F5544_RS39080_thiopeptideNZ_CP058322.1 Thiopeptides: HXZ27_RS30230_thiopeptideNZ_LT607411.1 Thiopeptides: GA0074695_RS29200_thiopeptide

Thiopeptides: IEY98_RS22980_thiopeptide

SCTTCECSCSCSS

62.5% NZ_LT907981.1: Thiopeptides: CPH93_RS17870_thiopeptide

SCTTCECSCSCSS

SCTTC CS CSS

SCTTCICSGRCSS

MIBiG 3.1 matches for ADH93699.1

18.2% BGC0000563 (AEA03263.1): Lanthipeptide: venezuelin

TCEC

17.9% BGC0000517 (BAB04173.1): Lanthipeptide: haloduracin β or haloduracin α

TCECSCSCS

T EC SC+

TVECMPSCN

15.4% BGC0002110 (QKV49894.1): Lanthipeptide: birimositide

CTTCECSCSC

C T +C+ C

CPTTKCTSKC

with 4 more sequence matches

14.7% BGC0000535 (ADJ56352.1): Lanthipeptide: nisin A

TCECSCSCS

TC CS S

TCHCSIHVS

13.3% BGC0002110 (QKV49893.1): Lanthipeptide: birimositide

TCECSCSCS

T EC +C+

TKECQPNCN

10.8% BGC0001229 (SBI_06989): Lanthipeptide: SBI-06990 A1 or SBI-06989 A2

CTTCECSCSC

C T +C+ C

CPTTKCTSRC

7.7% BGC0001229 (SBI_06990): Lanthipeptide: SBI-06990 A1 or SBI-06989 A2

ECSCSCS

EC +C+

ECQPNCN

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Types:

Type I: Featuring genes encoding indolic acid side ring (series e)
Type II: Featuring the gene coding for quinaldic acid moiety formation (series a, b, and c)
Type III: No genes for synthesizing L-Trp derivatives (series d)

Thiopeptides

Core peptide predictions

ADH93699.1

Putative Type I

Cleavage pHMM score: 11.30

RODEO score: 24

Monoisotopic mass: 1299.4 Da

Molecular weight: 1300.4 Da

Predicted peptide features:
Central ring: pyridine tetrasubstituted (hydroxyl group present); second macrocycle

Macrocycle: 26-member

Considering maturation reactions

Monoisotopic mass: 1277.2 Da

Molecular weight: 1278.3 Da

Alternative weights: (assuming N unmodified Ser/Thr residues); 1318.5 Da N = 1; 1336.5 Da N = 2; 1354.5 Da N = 3; 1372.5 Da N = 4; 1390.5 Da N = 5; 1408.6 Da N = 6; 1426.6 Da N = 7