Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0000612.1:
Cluster 1RiPP121168

BGC0000612, thiocillin I / thiocillin II biosynthetic gene cluster from Bacillus cereus. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: Thiocillin I
PubChem ID: 6446118
SMILES string: Copy to clipboard
Molecular formula: C48H49N13O10S6
Average molecular mass: 1160.361 Da
Molecular activity: Antibacterial

Class-specific details

Biosynthetic class(es):
RiPP
RiPP subclass:
Thiopeptide (cyclic)
Peptides in this cluster:
AAP11957
RiPP core peptide sequence(s): SCTTCVCTCSCCTT
Leader peptide length: 38
Follower peptide length: 0
Peptidase(s) involved in precursor cleavage:
AAP11958
RiPP core peptide sequence(s): SCTTCVCTCSCCTT
Leader peptide length: 38
Follower peptide length: 0
Peptidase(s) involved in precursor cleavage:
AAP11959
RiPP core peptide sequence(s): SCTTCVCTCSCCTT
Leader peptide length: 38
Follower peptide length: 0
Peptidase(s) involved in precursor cleavage:
AAP11960
RiPP core peptide sequence(s): SCTTCVCTCSCCTT
Leader peptide length: 38
Follower peptide length: 0
Peptidase(s) involved in precursor cleavage:

Gene cluster description

thiocillin I / thiocillin II (BGC0000612). Gene Cluster 1. Biosynthetic class = RiPP. GenBank AE016877, positions 4977401-4998568. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Detailed annotation

BC_5087 leader / core peptide, putative Class I
MSEIKKALNTLEIEDFDAIEMVDVDAMPE - NEALEIMGADhaCDhbDhbCVCDhbCDhaCCDhbDhb
BC_5088 leader / core peptide, putative Class I
MSEIKKALNTLEIEDFDAIEMVDVDAMPE - NEALEIMGADhaCDhbDhbCVCDhbCDhaCCDhbDhb
BC_5089 leader / core peptide, putative Class I
MSEIKKALNTLEIEDFDAIEMVDVDAMPE - NEALEIMGADhaCDhbDhbCVCDhbCDhaCCDhbDhb
BC_5090 leader / core peptide, putative Class I
MSEIKKALNTLEIEDFDAIEMVDVDAMPE - NEALEIMGADhaCDhbDhbCVCDhbCDhaCCDhbDhb

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies
MIxS-compliance:Unknown
Contact for this cluster:Jan Claesen (UCSF)

Literature references

1. Wieland Brown LC et al. (2009) Thirteen posttranslational modifications convert a 14-residue peptide into the antibiotic thiocillin. Proc Natl Acad Sci U S A 106(8):2549-53. doi:
2. Liao R et al. (2009) Thiopeptide biosynthesis featuring ribosomally synthesized precursor peptides and conserved posttranslational modifications. Chem Biol 16(2):141-7. doi: 10.1016/j.chembiol.2009.01.007.