BGC0000615 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000615 (Nocardiopsis sp. TFS65-07)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	thiopeptide	1	35,080	TP-1161	ribosomal:RiPP:Thiopeptide	100%

BGC0000615 - Region 1 - thiopeptide

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Location: 1 - 35,080 nt. (total: 35,080 nt) Show pHMM detection rules used

Region on contig edge.

thiopeptide: ((YcaO or TIGR03604) and ((thio_amide and (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or Lant_dehydr_N or PF07366 or PF06968 or PF04055) or thiostrepton)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

MIBiG comparison

KnownClusterBlast

Thiopeptides

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 35,081
Label		BGC0000615

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000615	0.94	RiPP	TP-1161	Nocardiopsis sp. TFS65-07
BGC0002311	0.30	RiPP	cutimycin	Cutibacterium acnes KPA171202
BGC0001472	0.26	RiPP	berninamycin A	Streptomyces bernensis
BGC0001146	0.24	RiPP	cyclothiazomycin C	Streptomyces sp. NRRL WC-3908
BGC0001860	0.23	RiPP	thiomuracin	Thermobispora bispora DSM 43833
BGC0002584	0.23	RiPP	azolemycin B, azolemycin A, azolemycin D, azolemycin C	Streptomyces sp. FXJ1.264
BGC0002120	0.23	RiPP	nocathioamide A	Nocardia terpenica IFM 0706
BGC0001145	0.23	RiPP	cyclothiazomycin b1	Streptomyces mobaraensis NBRC 13819 = DSM 40847
BGC0000605	0.22	RiPP	GE37468	Streptomyces sp. ATCC 55365
BGC0000613	0.22	RiPP	thiomuracin A	Nonomuraea sp. Bp3714-39

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000615	0.98	RiPP	TP-1161	Nocardiopsis sp. TFS65-07
BGC0000607	0.65	RiPP	micrococcin P1	Macrococcus caseolyticus
BGC0002311	0.64	RiPP	cutimycin	Cutibacterium acnes KPA171202
BGC0001472	0.59	RiPP	berninamycin A	Streptomyces bernensis
BGC0001146	0.57	RiPP	cyclothiazomycin C	Streptomyces sp. NRRL WC-3908
BGC0001860	0.56	RiPP	thiomuracin	Thermobispora bispora DSM 43833
BGC0002584	0.56	RiPP	azolemycin B, azolemycin A, azolemycin D, azolemycin C	Streptomyces sp. FXJ1.264
BGC0002120	0.56	RiPP	nocathioamide A	Nocardia terpenica IFM 0706
BGC0001145	0.56	RiPP	cyclothiazomycin b1	Streptomyces mobaraensis NBRC 13819 = DSM 40847
BGC0000605	0.55	RiPP	GE37468	Streptomyces sp. ATCC 55365

Similar known gene clusters from MIBiG 4.0

Thiopeptide predictions

Thiopeptide(s)

tpaA

Leader:

MNKDIDLSAIEISDLISETEQSDDALSQVMAA

Core:

SCTTTGCACSSSSSST

Core with dehydrations:

DhaCysDhbDhbDhbGCysACysDhaDhaDhaDhaDhaDhaDhb

antiSMASH-DB 4.0 matches for tpaA

100.0% NC_018524.1: Thiopeptides: B005_RS25790_thiopeptide

SCTTTGCACSSSSSST

47.1% NC_017803.1and 2 others:

NZ_BMKQ01000001.1 Thiopeptides: IEX42_RS14885_thiopeptideNZ_CP032698.1 Thiopeptides: DWB77_RS14290_thiopeptide

Thiopeptides: ACPL_RS43315_thiopeptide

SCTTTGCAC

SCTT+GCAC

SCTTSGCAC

MIBiG 3.1 matches for tpaA

25.0% BGC0000480 (ACA04484.1 (core 1)): Cyanobactin: tenuecyclamide A or tenuecyclamide C

TGCAC

TGC C

TGCMC

10.8% BGC0001229 (SBI_06989)and 1 other:

BGC0002110 (QKV49894.1) Lanthipeptide: birimositide

Lanthipeptide: SBI-06990 A1 or SBI-06989 A2

CTTTGC

C TT C

CPTTKC

tpaX

Leader:

MNHEIDLSAIEISDLVHEVEQGEDTFSQVMAA

Core:

SCVGTACTCSSTSSST

Core with dehydrations:

DhaCysVGDhbACysDhbCysDhaDhaDhbDhaDhaDhaDhb

antiSMASH-DB 4.0 matches for tpaX

100.0% NC_018524.1: Thiopeptides: B005_RS25795_thiopeptide

SCVGTACTCSSTSSST

43.8% NZ_BMSO01000004.1: Thiopeptides: IE184_RS11980_thiopeptide

SCVGTAC

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Types:

Type I: Featuring genes encoding indolic acid side ring (series e)
Type II: Featuring the gene coding for quinaldic acid moiety formation (series a, b, and c)
Type III: No genes for synthesizing L-Trp derivatives (series d)

Thiopeptides

Core peptide predictions

tpaA

Putative Type III

Cleavage pHMM score: 22.40

RODEO score: 30

Monoisotopic mass: 1468.5 Da

Molecular weight: 1469.5 Da

Predicted peptide features:
Central ring: pyridine trisubstituted

Macrocycle: 35-member

Alternative weights: (assuming N unmodified Ser/Thr residues); 1487.5 Da N = 1; 1505.6 Da N = 2; 1523.6 Da N = 3; 1541.6 Da N = 4; 1559.6 Da N = 5; 1577.6 Da N = 6; 1595.7 Da N = 7; 1613.7 Da N = 8; 1631.7 Da N = 9; 1649.7 Da N = 10; 1667.7 Da N = 11

tpaX

Putative Type III

Cleavage pHMM score: 23.70

RODEO score: 25

Monoisotopic mass: 1480.5 Da

Molecular weight: 1481.6 Da

Predicted peptide features:
Central ring: pyridine trisubstituted

Alternative weights: (assuming N unmodified Ser/Thr residues); 1499.6 Da N = 1; 1517.6 Da N = 2; 1535.6 Da N = 3; 1553.7 Da N = 4; 1571.7 Da N = 5; 1589.7 Da N = 6; 1607.7 Da N = 7; 1625.7 Da N = 8; 1643.8 Da N = 9; 1661.8 Da N = 10