LOCUS       BGC0000622              5494 bp    DNA     linear   BCT 23-JUL-2016
DEFINITION  Bacillus megaterium plasmid pBM309 P91, P293A, P62, P85, P293B,
            P188, and P73 genes, complete cds; tRNA-Cys gene, complete sequence;
            and P185 gene, complete cds.
ACCESSION   BGC0000622
VERSION     BGC0000622
KEYWORDS    .
SOURCE      Priestia megaterium
  ORGANISM  Priestia megaterium
            Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Priestia.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-07 20:03:58
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..5494
                     /db_xref="taxon:1404"
                     /mol_type="genomic DNA"
                     /organism="Priestia megaterium"
                     /plasmid="pBM309"
                     /strain="216"
     subregion       1..5494
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000622"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..5494
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     CDS             396..671
                     /codon_start=1
                     /note="megacin immunity protein"
                     /product="P91"
                     /protein_id="ABS44966.1"
                     /transl_table=11
                     /translation="MTRDTKAARILSIIMLAIVVIVLIMTEFVNVKSTGSTASLYYAYS
                     LAPIVGLILSLFGTKGKFRIVFSLIFLAFIVWFYVVPAMNFLNGDF"
     CDS             complement(712..1593)
                     /codon_start=1
                     /note="megacin A-216"
                     /product="P293A"
                     /protein_id="ABS44967.1"
                     /transl_table=11
                     /translation="MSTVEVTNIPEVFKNFNEYNETVDLALLIVSDDIQVNKDVIIEQR
                     GFISKVAFPKERPEEDEVVALVPDEEQTSMEDQEVTEEPIELVDYTQKTLYTKYANNLY
                     VKVMETVNDDDETDKLVHVSAVYLQEVNGTFIRKVIAYEDGVQFIESEVVPGEGEEIDP
                     ETELTIPNEAIEVTPDEGILPQVKLPVPCFNNSTGCCTFSNNGKTVHYKWCGPGCSGTG
                     ACTTANTKNGLDKCCCNHDKCYGQTKTYPNRCKCDLSLLNCSVSKRDPGSDRVWSAFVF
                     KRLYYKCASHGM"
     CDS             complement(1697..1885)
                     /codon_start=1
                     /note="hypothetical protein"
                     /product="P62"
                     /protein_id="ABS44968.1"
                     /transl_table=11
                     /translation="MKEIEISVKEYNDLKKDLLNIVKELDMCANEKRNMYQDIALCYTV
                     HLNDMKKIMKNKFNLKI"
     CDS             complement(1888..2145)
                     /codon_start=1
                     /note="glutaredoxin type protein"
                     /product="P85"
                     /protein_id="ABS44969.1"
                     /transl_table=11
                     /translation="MESEIIVFTQEGCIYCKKQKEWLSQNNIEYQEKNVDKDDEYRLEL
                     LERKIMGVPYTIIRFLEDNEKEVVVGGFDVKKLERLLLEY"
     CDS             complement(2277..3158)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075:alkaline serine protease, subtilase family
                     (Score: 236.3; E-value: 1.4e-71)"
                     /gene_kind="biosynthetic-additional"
                     /note="putative serine protease"
                     /product="P293B"
                     /protein_id="ABS44970.1"
                     /transl_table=11
                     /translation="MKDLNYGLEMIGVKNMWKHSFLGEGVVVAVIDSGAEKEHPAIHSN
                     IIGGFNFTNDDGGEENKYIDYIGHGTHVAGIIAGYDRDLKKIIGVAPLAKLLILKIIDK
                     DGQATIDNACKAIEYALNWRGENGEKVNVMNMSFGTNKDNEHLKSLIKETYSENVIMVA
                     SSGNYGDGNALTNELLYPAYYKEVIEVGAVKQNFEIYDYSNSNDEIDFVAPGYKILSSY
                     LNETYVKLSGTSMAAPHVSGAMALLINKFQKEDKEINYKSFYSYLRKDAKKLNYPITLQ
                     GHGLIQFKNSKI"
     CDS             3361..3927
                     /codon_start=1
                     /note="putative RNA polymerase sigma factor"
                     /product="P188"
                     /protein_id="ABS44971.1"
                     /transl_table=11
                     /translation="MNTQKTIQQFFNSHKKGLNKPIIKEFLKDDKNYEPLIQYILHPTE
                     KNQLKVDTAFKKHFQEIRDIKFYSSLIRFFSIDYDKRVRKLSNRFLLTLDQPIGNDHES
                     TIKDHIVDPQPQIPHEEGKSLSSLVENRALIKGLKTLTDKQLQVLELIYVKDLTNAEIA
                     KKMNSTTQNVCNTHRKAIRKLKSFF"
     CDS             3971..4192
                     /codon_start=1
                     /note="hypothetical regulatory protein"
                     /product="P73"
                     /protein_id="ABS44972.1"
                     /transl_table=11
                     /translation="MSTQNSSKLRLKMAEELITKFEGHIYKSLSKTKPEEREDLAQEIR
                     LKIVEKVFNSNFNEAPGFFDVLLNDDYY"
     tRNA            4279..4345
                     /note="putative"
                     /product="tRNA-Cys"
     CDS             4381..4938
                     /codon_start=1
                     /note="putative cell wall amidase"
                     /product="P185"
                     /protein_id="ABS44973.1"
                     /transl_table=11
                     /translation="MYKIVLDPGHGGEDPGAISLENGLREKDITLKIALYAKEFLLKNS
                     LARVKLTRENDVFIKLRERSKIAKRFKADFFVSIHVNAGGGTGFESYIYLETKDLTLAF
                     QQSLHNEIINTLHHQHESIPNRGLKRANYAVLRETSMPAVLTENLFIERDFELLKDEMF
                     LKDLGEAHARGIAKFLKLETLI"
ORIGIN
        1 aagcttggtt ataggcagtg acacgcttaa aagccctttt tattgagggc ttttctcttt
       61 cataaaatta tctaaaatgc tacaaatgtt tttgtttttc agtgtccgaa attaattgtc
      121 cgcatttatc attaaaggtt gaaatagctt ctgtcaaaat ttcaattttc ttttcgaatc
      181 taagaagcaa gtatccagca agtaccatag ggaaaccaaa gttacctagc aacgcagtcc
      241 aaatttgtac actttccatc agtacgatca cctccttaac taaataagtg aaagaaaaag
      301 tgaaaatgaa actctataaa attatgaaaa aaataacatt tttattgatt taaaaagggt
      361 aaaatggtaa aatctcatgt gggaggagat ttttaatgac tagagataca aaagcagcaa
      421 gaatcctatc gattatcatg ttagcaatag ttgttattgt tttaatcatg actgaattcg
      481 taaatgtaaa aagtacaggt agtacagcgt ccttatatta tgcttattcc ctcgcaccaa
      541 ttgtcggatt aatactttct ttatttggca caaaaggaaa gtttagaatt gttttttcac
      601 tgatttttct tgcctttatt gtttggtttt atgtggttcc tgctatgaat tttttaaacg
      661 gggattttta aaaggaaaaa gagaccaaga aaaatggtct cttttttaat cttacatacc
      721 atgagaagcg catttataat aaagtctttt gaaaacaaaa gcgctccata ctctatctga
      781 accagggtca cgcttactaa cagagcaatt taaaaggctc aaatcacatt tacaacgatt
      841 tggatatgtt tttgtctgtc cataacattt atcatgatta cagcagcatt tatctaaacc
      901 atttttagta tttgcggttg tacaagcacc tgtaccagaa catccgggtc cacaccattt
      961 gtaatgaact gttttaccat tgttagaaaa tgtacagcaa cctgttgagt tattaaagca
     1021 tggtacaggc aatttaacct gcggtaaaat cccttcatct ggagtaactt caatagcttc
     1081 atttggaata gttaattcag tttctggatc aatttcttca ccttcaccag gaacaacttc
     1141 actctcgata aattgtacac cgtcttcata ggcaataact ttcctaataa atgtaccatt
     1201 aacttcttga aggtaaactg cactaacatg aacaagtttg tcagtttcat catcgtcatt
     1261 aactgtttcc ataaccttca catataaatt atttgcatac tttgtataca aagttttttg
     1321 tgtgtaatca actaattcaa ttggttcttc agtaacttct tgatcttcca ttgatgtttg
     1381 ctcttcatct ggaacaagag caactacttc atcctcttct ggacgctctt taggaaacgc
     1441 tacttttgaa atgaaccctc tttgctcaat aataacatct ttgttaactt gaatatcgtc
     1501 actaacaatt aatagtgcta aatctactgt ttcattgtat tcgttaaagt ttttaaaaac
     1561 ttcaggaata ttggtaacct ctactgtact catatttctc ctcctatatg gtctataata
     1621 agttagctaa caaaagaaat ttcctttgtt ttatataaag tgatgcgact ataaaaaaag
     1681 aaaccagtaa gaaaaattaa atttttaaat taaatttatt tttcattatt tttttcatat
     1741 cgttaaggtg gacagtgtag caaagtgcaa tatcctgata catattcctc ttttcgttag
     1801 cacacatatc caattcctta acaatgttta ataagtcttt ttttaaatcg ttatattcct
     1861 taactgaaat ctcgatttcc ttcatactta atactccaat aatagtcgct ctaacttttt
     1921 cacatcaaat ccaccaacaa caacctcttt ttcattgtcc tcaaggaatc ttataattgt
     1981 atatggaaca cccatgattt ttcgttctag aagttctaaa cggtattcat catccttatc
     2041 aacatttttt tcttgatatt caatattgtt ttgggacaac cattcttttt gctttttaca
     2101 ataaatacaa ccttcctgtg taaagacaat aatttctgat tccattagac ttcacccttt
     2161 caattaacta aaaaaaagca gtaattagat ataaaccatt taaaaattgg aaacctaatt
     2221 tactgctggc tatagttaaa taagtgaaat aactctatat taaagaaacc tcttatttaa
     2281 atttttgaat ttttaaattg aataagaccg tgaccttgaa gagttatagg atagtttaat
     2341 ttttttgcat cctttcttaa atatgagtaa aaacttttat aatttatttc tttatcttct
     2401 ttttgaaatt tatttattag taaggccata gcgcctgata catggggggc tgccatagag
     2461 gttcctgata atttcacata agtttcattt aaataagagg ataaaatctt ataacctgga
     2521 gctacaaagt caatttcgtc gttggaatta gagtaatcat atatttcaaa gttttgttta
     2581 actgcaccaa cttcaataac ttctttataa taagcaggat aaagaagttc atttgtcaaa
     2641 gcatttccat ccccatagtt gccagacgaa gcaaccataa tcacattttc actatatgtt
     2701 tctttaatta aagactttaa atgctcgtta tctttattgg taccaaacga catattcatt
     2761 acattaactt tttcgccatt ttccccacgc caattcagag catactctat tgccttacat
     2821 gcattatcaa tagtagcttg accatctttg tctattattt ttaagattag taattttgcc
     2881 aaaggagcaa ctcctataat cttctttaaa tcacgatcat agcctgcaat gataccagct
     2941 acatgagttc catgcccaat gtaatcaata tatttatttt cttctccccc atcatcatta
     3001 gtaaaattaa atccacctat aatatttgaa tgaatagccg gatgctcttt ttcggcaccg
     3061 gaatcaataa cagcaactac tacaccctct cctagaaagg aatgtttcca catattttta
     3121 accccgatca tttctagacc atagtttaaa tccttcatct ttttcacctc tcttatttaa
     3181 gtgaaagaca aatcatgaaa gaaactatca tgtcgaaaag tctgaattat agaacaacta
     3241 acaacaggaa catttgttct gttatttggt ttataatggt atagttggtt tcacttagta
     3301 aagagagact gcttttacat gagtccattc tactaaagat tatagaaagg aatgtatgta
     3361 atgaacaccc agaaaactat tcagcaattt tttaacagtc acaaaaaagg attaaacaaa
     3421 cctattatta aggaatttct taaagacgac aaaaactatg agcctctcat tcaatacatt
     3481 cttcatccaa ccgaaaagaa tcaattaaaa gtagatacag catttaaaaa acatttccaa
     3541 gaaattagag atattaagtt ctatagcagc ctaattagat ttttttccat tgattatgat
     3601 aaaagggttc gtaagctaag taatcgattt ttattaacct tagatcaacc aatagggaat
     3661 gatcacgaat ctactattaa agatcatata gtagatcccc aaccacaaat tccacatgaa
     3721 gagggaaagt cacttagttc tcttgttgaa aatcgggctc ttattaaagg cctaaaaaca
     3781 cttacggata aacaattgca agttttagaa cttatttatg taaaagactt aactaatgct
     3841 gaaattgcaa aaaagatgaa ttctacaact caaaatgttt gtaacactca tagaaaagct
     3901 atacgaaaat taaaatcttt cttttaataa acgaaaaaac tactttagtt aataattggg
     3961 aggaaaagtt atgtctacac agaatagttc caaattaaga ttaaaaatgg cagaagaatt
     4021 aataactaaa tttgaagggc atatctacaa aagtttaagt aaaactaagc ctgaggagcg
     4081 cgaggattta gcacaagaaa tacgcttgaa aattgttgaa aaagtcttca atagtaactt
     4141 taatgaagca cctggattct ttgatgtttt attaaatgat gattattatt aaaaaaagtt
     4201 gccatttggt caactttttt tcttttaaag gtttcttatt gatttttagt aacacttatt
     4261 taattataat acggtggcat agccaagtgg taaggcaagg gtctgcaaaa ccttgatcgc
     4321 cggttcaaat ccggctgtca cctttgaata gattaactcc tttttaagaa ggtgaaagat
     4381 atgtataaaa ttgttttaga tcccgggcat ggcggagaag acccaggagc tatatcatta
     4441 gaaaatggac ttagagaaaa agatattaca ctaaaaatcg ctctctatgc taaagaattt
     4501 ttattaaaaa attctttagc aagggttaag ttaactagag aaaatgacgt gtttattaag
     4561 ttgcgagaaa gaagtaaaat agccaagcgc tttaaagcag atttttttgt ttcaattcat
     4621 gttaatgcag gtggaggaac tggttttgaa agctatattt atttagaaac taaagacctt
     4681 acattggcat ttcaacaatc tttacacaat gaaattataa acactctgca tcaccaacat
     4741 gaaagtattc cgaatcgtgg actaaaaaga gccaattatg cagttctgag agagacatct
     4801 atgccggcag tgttaacgga aaacctattt atcgaacgtg attttgaact tttaaaggat
     4861 gaaatgttct taaaagattt aggcgaagca catgcaagag gaattgctaa atttttaaaa
     4921 ttagaaacac tgatttaata ttagataact gactgcctaa acaaaacaac tatctttata
     4981 aaggtagttg ttttatttag gttgatttgc tcttactaaa ttagatttta tgagtttaaa
     5041 ggattggata aggaacacat ttcaaacttg aacctgatct tttttcattt tcagactgta
     5101 tatcatatcc atttctaatt tatataagat tgattatgtt tagcttcact tctgaccata
     5161 tccttaagta aatttacacc tatttaatta aaatagcttt atcatttata caccttgtag
     5221 aaaaatgatc catttgtcta cattaaaagt aaaagataaa aaaagtaaaa gataaaagtt
     5281 tgtatttata taaagtgatt cagatattat ccaattaaat attttattta tcttagtttt
     5341 ttaccttaag gagaattacc ataaatttat tttctgccaa tccttttctg gtatttctct
     5401 attgaaataa tcaattggtt caactctata acctttggtc ttaaaactta tggtctcttc
     5461 tacatagggg ggattatgtg gaccatcaaa gctt
//
