LOCUS       BGC0000624              7759 bp    DNA     linear   BCT 21-SEP-2009
DEFINITION  Lactobacillus salivarius salivaricin CRL1328 biosynthetic gene
            cluster, complete sequence.
ACCESSION   BGC0000624
VERSION     BGC0000624
KEYWORDS    .
SOURCE      Ligilactobacillus salivarius
  ORGANISM  Ligilactobacillus salivarius
            Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
            Ligilactobacillus.
COMMENT     ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2024-10-17 13:56:50
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7759
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..7759
                     /db_xref="taxon:1624"
                     /mol_type="genomic DNA"
                     /organism="Ligilactobacillus salivarius"
                     /strain="CRL1328"
     misc_feature    1..7759
                     /note="salivaricin CRL1328 biosynthetic gene cluster"
     subregion       1..7759
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="ribosomal"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..7759
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     CDS             complement(47..208)
                     /codon_start=1
                     /product="putative peptide"
                     /protein_id="ABQ84440.1"
                     /transl_table=11
                     /translation="MPSWLSNINSIVTINIHHLRGLLVTISTIPTDIRKIEYIMPLPRS
                     TLINPISI"
     CDS             313..570
                     /codon_start=1
                     /product="bacteriocin-like prepeptide"
                     /protein_id="ABQ84441.1"
                     /transl_table=11
                     /translation="MLKKLWETWLDGGFIRGKKRYVIAPVIWALLIPLGIWLFGNEEMS
                     YLDYIQTPKMIIVTIFCLVGGSTLLYLLDTAMKVNKHMKG"
     CDS             576..770
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /product="salivaricin CRL1328 alpha peptide"
                     /protein_id="ABQ84442.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 1.2e-17,
                     bitscore: 53.8, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMKEFTVLTECELAKVDGGKRGPNCVGNFLGGLFAGAAAGVPLGP
                     AGIVGGANLGMVGGALTCL"
     CDS             788..994
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /product="salivaricin CRL1328 beta peptide"
                     /protein_id="ABQ84443.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 3.6e-12,
                     bitscore: 36.3, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNLDKRFTIMTEDNLASVNGGKNGYGGSGNRWVHCGAGIVGGAL
                     IGAIGGPWSAVAGGISGGFTSCR"
     CDS             1137..1304
                     /codon_start=1
                     /product="putative salivaricin immunity protein"
                     /protein_id="ABQ84444.1"
                     /transl_table=11
                     /translation="MVFWVTVIVAYSIFISIYTSLKEDGHYKLFDEKVIEKNKKISYVR
                     AWLLNFMWWL"
     CDS             1424..1540
                     /codon_start=1
                     /product="salivaricin induction peptide"
                     /protein_id="ABQ84445.1"
                     /transl_table=11
                     /translation="MKFEVLTEKKLQKIAGGATKKGGFKRWQCIFTFFGVCK"
     CDS             1540..2829
                     /codon_start=1
                     /product="sensory transduction histidine kinase"
                     /protein_id="ABQ84446.1"
                     /transl_table=11
                     /translation="MVNLNSTSGYIVATLILFINTFCRYSFFYLLTPTRKSKKYNLLFI
                     LLTFPVILLINGVGDIIGFFAPFIEIFIFYIVYRQLTLDYRLCGRLLMLDLFSYITFGA
                     SALFFTLFTNYYTMVSVIILDVSLNIFEYIILTKTTIIEKLDDEYGLLYFYLILYIWIS
                     SMVIYYLTTYVFNIDAVSYIIAMVVVVSQLFFSLFVYSINKKIRQDKLSLQQINDLKIY
                     TQQLESSQRGLRKFKHDYQNMLLSLKLSAKKSHDKELIDKLAEYSSKTLEDKVLWQFND
                     VDNVKDELLKSLFISKLNRIFQNNIQYSFECRIVIENLSNKYNSFDIVRILGIVYDNAI
                     EESLEFGDEAKIDTMIYQENGELEIEIRNRYRDTDLTIQDIKKSGFTTKKNHDGLGLAN
                     IEELSQSYNDIFINYQIKDGWFTFLMVMSI"
     CDS             2843..3637
                     /codon_start=1
                     /product="salivaricin response regulator"
                     /protein_id="ABQ84447.1"
                     /transl_table=11
                     /translation="MEEYGIIICDDEKDQVRRIHDLVKYAIWSLEDSADSKGKKFKITL
                     EANSYIEVADYLEDNKVDSGVYFLDIELSRDRADKDGIDLAEFIRQRDPNAQIIFITAY
                     ENLAAMTYRRRTGALNFITKNDSDEVIQKRLNETLSLAARRIRESHAIKKMTFSYKMGR
                     RIINENINDILYIITTPKSHRLKIVTVNGMGEFTGDIKKLEAENDFLEKISQSCLANPS
                     NIVEINLSTRVISFKDGYKETFPRRSKAKMKSLIERYNLKTV"
     CDS             3675..3914
                     /codon_start=1
                     /product="hypothetical membrane protein"
                     /protein_id="ABQ84448.1"
                     /transl_table=11
                     /translation="MVETGIIILLIVGFMAISRLKVNFTRNSKYAGFLLLILLNLNGLM
                     RTPMLTMRSLILLGTLMIFVVMLIKEIRQSRKYS"
     CDS             4064..6223
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR01193"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288:ABC
                     transporter related protein (Score: 314.6; E-value:
                     2.1e-95)"
                     /gene_kind="biosynthetic"
                     /product="salivaricin ABC-transporter"
                     /protein_id="ABQ84449.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 3.5e-45, bitscore:
                     142.8, seeds: 36, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR01193 (E-value: 0.0, bitscore: 1077.3,
                     seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLRKYRKDYVSQVDESDCGVASLAMILKHYGSALSLAYLRNIAKT
                     TMEGTTALGLVKTAQALGMETQAVKADMSLFGMKDITYPFIVHVVKEETLLHYYVVLKA
                     TPRYILIADPAPDVGITKMSYERFEKEWTGITLFIVPGIEYKPTKETRQSLISLFSKLF
                     KQKQLIVNIVLAALLITIISILGSYFLQGIIDTYIPNGMHSTLAIIALGLLVIYVFNSI
                     FTYARDFLLAILGQRLSIEIILSYIRHIFELPMEFFATRKTGEIVSRFNDASKIIDALA
                     STVVSIFLDVSIVVIMGIILAIQNLKLLGITLITLPIYTIIILTFTKAFERLNNKQMES
                     NAILSSSIIEDIQGIETIKALNSEQMRYRKIDSQFVDYLRKSLAYIKTDSLQQALKLFI
                     QQTLNVIVLWLGANLVISNQLSIGQLMTFNALLSYFVNPLQNIINLQPKLQSARVAHNR
                     LNEVYLVESEFKDRKAVHDVTQLQGDIIFKDVDYHYGYGENVLDKVNLTLKQGEKLTIV
                     GMSGSGKSTLVKLLVNFFAPVSGSIKLNNHDINNIDKHALRSYINYIPQNPYIFTGTIE
                     DNLRLGNRSNVTDEDILMACKIAMIDIDIAKMPLQLKTELDENANILSGGQKQRITIAR
                     ALLSPAQVLIFDESTSGLDAITEKKLIDNLMKLKSKTIIFIAHRLSIAKRTNNILVLDS
                     GKIVEKGRHDELLAKKGYYYNLVNS"
     CDS             6240..7394
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1029:RND family
                     efflux transporter MFP subunit (Score: 56.4; E-value:
                     3.9e-17)"
                     /gene_kind="transport"
                     /product="salivaricin export accesory protein"
                     /protein_id="ABQ84450.1"
                     /transl_table=11
                     /translation="MEDKFLESSEFYSARFRNFSTMIIIPFTILLLVVVIFSFFGKKEI
                     TIQGAGTLEALGNNPVIQSTVNSPIEKNYLKEGKYVKKGDTLLIYNNVSIQDKGKLYNS
                     QKEILDRQLAALNLLKQGVQDNHDSFGEDDEFGYRNDLKSYLQQRNTYLTENTMIQDKS
                     KNDKAKQSSINNELKKQSGSATSIADFDSSKYDIEINNQKISDLQSSKLSSIDQEIIKI
                     KQNISELNANINENNNTNREYKVKAVRDGILHINDEYSESHYISAGANLAQILPVLKNQ
                     KTLKVKAYVSTADISSIKVGQKVRFKVTRNVSKPIILTGKIYKISISPVEIGKENYYQI
                     EAKTDIENTNKDLLKYGMQGRMVVITGKTTFFEYYKNKLFNIKQ"
     CDS             7510..7740
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="ABQ84451.1"
                     /transl_table=11
                     /translation="MNYDIPNFVKESPNYLKLFEKERIILKNNHDKWGIYFSEELGHPL
                     EYKENSGKVDYIYDFFLEAYSQADNDGEVWF"
ORIGIN
        1 agatgaagaa gacagataga atatttatgt attgcatagg tataggttag atacttatag
       61 ggttaatcaa tgtgcttcta gggagtggca taatatattc gatttttcta atgtctgtag
      121 ggatagtact tatagttaca agtaagccac gtagatgatg aatatttata gttacaatag
      181 aatttatatt agataaccaa ctgggcataa aataaggtat tttacgaaaa attcatattc
      241 aagtattaaa tggtactaca ataaaagcgt aagatatgtt tcatagatat tttagaaagg
      301 gtgtgttttt acatgctaaa aaaactatgg gagacttggt tggatggtgg atttattaga
      361 ggaaagaaga gatatgttat tgctccagtt atttgggcac tgttgattcc actaggaata
      421 tggctgtttg gtaatgagga aatgtcttat ttagattata ttcaaacgcc taaaatgata
      481 atagttacta ttttttgtct agtaggtggt agtacactac tttacttatt agatactgcg
      541 atgaaagtaa ataaacatat gaaaggatga ttatcatgat gaaggaattt acagtattga
      601 cagaatgtga gttagcaaag gttgatggtg ggaaacgtgg tcctaactgt gtaggtaact
      661 tcttaggtgg tctatttgct ggagcagctg caggagttcc acttggacca gctggtattg
      721 taggtggagc aaacttagga atggtaggcg gagcacttac ttgtttataa tataggagtg
      781 atttaaaatg aaaaatctag ataaaagatt cacaattatg actgaagata acttagcatc
      841 agtaaatggt ggtaaaaatg gttatggtgg tagtggaaat cgctgggttc actgtggagc
      901 tggcatcgta ggtggagctt taattggagc tatcggtgga ccctggtcag ccgtagcggg
      961 tggaatttct ggtggtttta caagttgccg ttaagataag tctaatgaat tagaaaataa
     1021 gaaataaatc tatttttctt caagtagtgt caagtgtaag ttatagtata tagctaaatt
     1081 tgacactact tttattattt ttaatataat atattttgag attaggaagt gagtatatgg
     1141 tattttgggt tactgtaata gttgcatata gtatctttat aagtatttat actagtctaa
     1201 aagaagatgg gcattataag ttatttgatg agaaagtcat agaaaagaat aagaaaataa
     1261 gttatgtgag agcgtggttg ttaaatttta tgtggtggct atagttttaa gaatttcatc
     1321 caaataagca ttaaaatgaa ccgaatatga taaaaaacga ataaagtaga ttaaaaaatg
     1381 ttaaactata tttgtaataa agataacaaa ggagagattt tttatgaagt ttgaagtgtt
     1441 aacagaaaag aagttacaga aaatagctgg gggagcaacc aaaaagggtg gctttaaaag
     1501 atggcaatgt atattcacat tttttggtgt ttgtaaataa tggttaattt aaattcaaca
     1561 tctggttata tagtagctac actaatttta tttataaata ctttttgtag atatagtttt
     1621 ttttatcttt tgacccctac taggaaaagt aagaagtata atttattatt tatacttctt
     1681 acttttcctg taattctttt aattaatggt gttggagata ttattggttt ttttgcacca
     1741 tttattgaaa tttttatttt ttatattgta tacagacaac taactttgga ttacagatta
     1801 tgtggaaggt tgttaatgct agatttattt tcctacataa cattcggtgc ttcagcatta
     1861 ttttttacat tatttacaaa ttattacaca atggtcagtg tgataatatt agatgtgtca
     1921 ttaaacattt ttgaatatat aattttaaca aagacgacta taatagagaa actagatgat
     1981 gagtatggat tattgtattt ttatttaatt ttatacatat ggattagttc aatggttatt
     2041 tactatctaa caacatatgt atttaatata gatgcagtat cgtatattat agctatggta
     2101 gttgttgtta gccaattgtt cttttcatta tttgtatatt ccattaataa gaaaatacgg
     2161 caggataaat taagtttaca acaaattaat gatctaaaaa tttatacaca acagttagaa
     2221 agtagccagc gaggtttacg taaatttaag cacgattatc aaaatatgct attaagcttg
     2281 aaactcagtg caaaaaaaag tcatgataaa gagttaatag ataaactagc tgaatattca
     2341 agtaagactt tagaagataa agtattatgg cagtttaatg atgttgacaa tgttaaagat
     2401 gaattattaa agagtctatt tatcagtaag ttgaatagaa tctttcaaaa caatattcaa
     2461 tattcttttg aatgtcgaat tgtgatagaa aacttgtcga ataagtataa ctcgtttgac
     2521 attgttagaa tattaggaat tgtgtacgat aatgcaattg aagaaagtct tgagtttggt
     2581 gatgaagcta agatagatac tatgatttat caagaaaatg gtgaacttga aattgagatt
     2641 agaaatcgtt atcgagatac cgatctaaca attcaagata taaaaaaatc aggttttaca
     2701 actaagaaga atcatgacgg attaggatta gctaatatag aagaattaag tcaatcttat
     2761 aatgatattt ttattaatta tcaaattaaa gatggttggt ttactttcct tatggttatg
     2821 agcatttaag gggtagttta atttggaaga atatggaatt attatttgtg acgatgagaa
     2881 ggatcaagtt agaaggatac atgatctagt taaatatgct atttggtctt tagaagattc
     2941 tgctgacagt aaaggtaaaa aatttaaaat aactttggaa gctaatagtt atattgaagt
     3001 agccgattat ttggaagata acaaagtaga tagtggagtt tattttttag atatcgagct
     3061 tagtagggat agagcagata aagatggcat agatttagct gaatttatta gacaaagaga
     3121 tccaaatgca cagataattt ttattactgc ttatgaaaat ctagcagcaa tgacctatcg
     3181 acgtcgcaca ggagcattga attttattac taaaaacgat agtgatgaag tgattcaaaa
     3241 gagactaaat gaaactttat cattagctgc aaggagaatt agggaatctc atgccataaa
     3301 gaaaatgaca tttagttata aaatgggcag aagaattatt aacgaaaata tcaatgatat
     3361 tctatatatt attaccactc ctaagtcaca tagattaaaa atagttacag ttaatggtat
     3421 gggtgagttt acaggcgata ttaagaagtt agaggcagaa aatgattttc tagaaaaaat
     3481 atcacaatct tgtttagcta atccaagtaa tattgttgaa attaacctgt caacaagggt
     3541 tatttctttt aaagatggat ataaagagac ttttcctaga agaagtaagg ctaaaatgaa
     3601 aagtttgatt gaaaggtata atttgaaaac agtttaattt ctgtgtgttt attgatatag
     3661 gtggtgaaaa tgacatggta gaaacaggta ttattatatt gctaattgta gggtttatgg
     3721 caatttcaag gttgaaagta aattttacaa gaaatagtaa gtatgcaggt tttctattgt
     3781 taatattact gaatttaaat gggcttatgc gaactcctat gttaacaatg agaagtttaa
     3841 tcttattggg aacattgatg atttttgtag ttatgttgat taaagaaatt agacaaagta
     3901 gaaaatatag ttaataaaga gggttggaac ttagagtatt ccagccttct tttttaacca
     3961 aatatgtatt aaaaatcgcc aaatttgata actaatacat aagataagag agaaatgata
     4021 tttttggtat aaaaataata atagaaaata agggggattt ttcttgttaa gaaagtatcg
     4081 aaaagattac gtttcacaag tagacgaatc tgattgtggc gtggcttcgt tggctatgat
     4141 tttgaaacac tatggatcag cactatcatt agcgtattta aggaacattg ctaagacaac
     4201 aatggagggg actacagctt taggtcttgt taagacagca caggctttag gaatggaaac
     4261 acaagccgta aaagctgata tgtctctttt tggaatgaaa gacattacat atccatttat
     4321 tgtacatgtg gttaaagaag agacgttatt acattattat gtagtattga aagcaacgcc
     4381 tagatatatt ttaattgctg atccagctcc agatgtaggg ataactaaaa tgtcgtatga
     4441 acgttttgaa aaagaatgga cagggattac tttattcatc gttccaggaa ttgaatacaa
     4501 gccgacaaaa gagactagac aatcattaat atcactattt agtaaattat ttaagcaaaa
     4561 gcaactaata gttaatattg tattggcagc tttgttaatt acaattatta gtatattggg
     4621 atcatatttt ttacagggaa taattgatac atatattcct aatggtatgc atagtacact
     4681 ggcaattatt gctttgggat tactagtaat atatgtgttc aattcaattt ttacatatgc
     4741 aagggatttt ttattagcaa ttttaggaca acgactttcg attgaaatta ttttaagtta
     4801 tattagacat atttttgagt tgcctatgga attttttgcg accagaaaaa ctggggaaat
     4861 agtttctcgt ttcaatgatg ctagtaaaat tatagatgct ttagcaagta ctgtggtatc
     4921 tattttttta gatgtaagta ttgtagttat tatgggaata atcttggcta ttcaaaatct
     4981 taaattacta ggaataactc ttatcacact tccaatttac acgattatta ttcttacatt
     5041 tacaaaggca tttgaaagat tgaataataa acaaatggaa agtaatgcga tacttagttc
     5101 atctattatt gaagatattc aaggaataga aacaataaaa gccttaaaca gcgaacaaat
     5161 gcgttaccgc aaaattgata gtcaatttgt ggactatttg cgtaaatcgt tagcatatat
     5221 aaaaacagac tcgttgcaac aagcattaaa gttatttatt caacaaacat taaatgttat
     5281 agtcctatgg ttaggggcaa atctggtaat tagtaatcaa ctctctatag gacaattaat
     5341 gacttttaac gcactattaa gttattttgt gaatccatta caaaatatca taaatttaca
     5401 gccaaaattg cagagtgcaa gggtagctca caatcgtttg aatgaggttt acttggttga
     5461 aagtgaattt aaagatagaa aggctgttca tgatgttact caacttcaag gagacattat
     5521 atttaaagat gttgattatc actatggtta tggtgaaaat gttttggata aggttaattt
     5581 gactttaaaa cagggagaaa aactaactat agtaggtatg agtggctctg gcaaatcaac
     5641 gttagttaaa ctattggtga atttttttgc tccagtttca gggagtataa aactaaataa
     5701 tcatgatatt aataatattg ataagcatgc actgcgttcc tatattaatt atataccaca
     5761 gaatccatat atttttaccg ggacaataga agataatctt agactaggta atcgatctaa
     5821 tgtgactgat gaggatattc ttatggcttg taaaatagca atgattgata tagatatagc
     5881 gaagatgcca ctacaattaa aaactgaatt agatgaaaat gctaatattt tatcaggtgg
     5941 acaaaaacaa agaattacaa ttgctagagc cttattatct ccagcacaag tattgatttt
     6001 tgatgaatct acaagtgggt tagatgctat tacggagaaa aaactaattg ataatttaat
     6061 gaaattaaag tcaaaaacta taatttttat tgcacataga ttatctatag ctaagcgaac
     6121 aaataatatt ctagtattag atagcggaaa aattgtagaa aagggtagac atgatgaatt
     6181 attagctaaa aaaggatact actacaattt agttaatagt taggggggag tattgtatta
     6241 tggaagataa atttttagaa agcagcgaat tttattcagc tagatttaga aacttttcaa
     6301 ctatgattat tattcctttt acaattttat tgttggtggt agtaattttt agcttttttg
     6361 gaaaaaagga gataactatc caaggtgcag gaacacttga ggcattggga aataatcctg
     6421 ttattcaatc aactgtaaat agtcctattg aaaaaaatta tttaaaagaa ggaaagtatg
     6481 taaaaaaagg agatacatta cttatttata ataatgtgag tatacaagat aaggggaaac
     6541 tgtataattc acaaaaagaa atcttagata gacagttggc agctttgaac ttacttaaac
     6601 aaggtgttca agacaatcat gattcttttg gagaagatga tgaatttggt tatcgtaatg
     6661 atttaaagag ttacctacag caaaggaata cgtatttaac agaaaataca atgatccagg
     6721 ataaatctaa aaacgacaaa gccaaacaaa gttcgattaa taatgagttg aagaaacaaa
     6781 gtgggtctgc tacaagtata gctgattttg attcgtctaa atacgatatt gagataaaca
     6841 atcaaaaaat aagtgattta cagtcaagta agttaagttc tattgatcaa gaaattataa
     6901 aaatcaagca aaatatttca gaattgaacg ctaatattaa tgaaaataat aatactaata
     6961 gagagtataa ggtaaaggct gttagagacg gaattttaca tattaacgat gagtatagtg
     7021 aaagtcatta tataagtgca ggggctaatt tagctcagat actaccagtt ttgaaaaatc
     7081 aaaaaacttt aaaagtaaag gcatatgtaa gtacagcaga tatttcctcc ataaaagtag
     7141 ggcaaaaagt aagattcaaa gtaactagaa acgtatctaa acctattatt ttgactggaa
     7201 aaatatataa gatatcgatt tctccagtag agataggaaa agaaaattat tatcaaattg
     7261 aagctaaaac agatattgaa aatactaata aggatttatt gaagtatggt atgcaaggac
     7321 gaatggttgt aattacagga aaaacaacat tttttgaata ttataagaat aagttattta
     7381 atattaaaca ataaaaattt tttaaatatt ccttactatt aaactactgg tagattttta
     7441 tttcgtaaag attagggtag gattattgta gattaacaag taaagatgag tgacatggaa
     7501 gaaaaatttt tgaattatga tatacccaat tttgtaaaag aatcacctaa ttacctaaaa
     7561 ctatttgaaa aagaacgtat catcctaaag aataatcatg ataaatgggg gatctacttt
     7621 agtgaggaat tggggcatcc attggaatat aaggaaaatt caggaaaagt agattatata
     7681 tatgattttt tcttggaagc ctactctcaa gctgataatg acggagaagt ttggttttaa
     7741 gatgaaatag cacatgaag
//
