LOCUS       BGC0000626              7622 bp    DNA     linear   BCT 26-APR-2014
DEFINITION  Bacillus thuringiensis strain As 1.1013 thuricin biosynthesis gene
            cluster, complete sequence.
ACCESSION   BGC0000626
VERSION     BGC0000626
KEYWORDS    .
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
COMMENT     ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2022-11-07 20:04:31
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7622
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     gene            1..168
                     /gene="thuA"
     CDS             1..168
                     /codon_start=1
                     /gene="thuA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) L_biotic_A"
                     /gene_kind="biosynthetic-additional"
                     /note="ThuA"
                     /product="thuricin precursor"
                     /protein_id="AHX39582.1"
                     /sec_met_domain="L_biotic_A (E-value: 3.6e-15, bitscore:
                     44.4, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="METEKYLQVVEDEEIEQLVGGAGPGWVETLTKDCPWNVPVACVTI
                     MGQRICKKCY"
     source          1..7622
                     /db_xref="taxon:1428"
                     /host="insect"
                     /mol_type="genomic DNA"
                     /organism="Bacillus thuringiensis"
                     /strain="As 1.1013"
     misc_feature    1..7622
                     /note="thuricin biosynthesis gene cluster"
     subregion       1..7622
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="RiPP"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..7622
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            260..3088
                     /gene="thuM"
     CDS             260..3088
                     /codon_start=1
                     /gene="thuM"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: DUF4135"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: LANC_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1070:lanthionine synthetase C family protein (Score:
                     687.1; E-value: 6.6e-208)"
                     /gene_kind="biosynthetic"
                     /note="ThuM"
                     /product="thuricin modification protein"
                     /protein_id="AHX39583.1"
                     /sec_met_domain="LANC_like (E-value: 3.9e-38, bitscore:
                     119.7, seeds: 47, tool: rule-based-clusters)"
                     /sec_met_domain="DUF4135 (E-value: 7.7e-82, bitscore:
                     264.0, seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNNSHGILNTIDNGSHSLVTKEYLEKNFVIFLEGFSCNDVIKDD
                     ENYNFQKIIQTYVVFFKNMIINKIDFFGNFEKVSESIMFDLEKRLYQVLRPSLIVYINK
                     LRVENALRGMDTNEEYKYFTKNFFHFKKFYMNFFKILPVLNEKIFTIIENEINYMIWLQ
                     GALEKDQKEIHKMSGFNIMHVEKVINLGDYHNNGKRTILLEDKFNNKVILKPVDLKNSI
                     LYHSLINQLNKDLNTDIFIPQVVNKRNYGYMEYINHESCESNEQIKDYYYNLGVVLSVM
                     YLINGSDIHNENIIAKQSSPVIIDFETLGSTLNPSVTEETSSFILSNSVLNSRMLPIRF
                     SGGREVIRDYSAIGRVMKSLVKTIKIKNEFTSNPIEIREETIVEDTVQNLPFLNDDIYE
                     YDNYIDDIIKGFENAYNSVLKNKEEYMCILKNSVSKWNYRKVYRNTKVYTLLLDRLNVP
                     DLLENKLKTIKYLRNILEKNIYLATRKDIIQKEIEALLSYDVPYFFNKYEWESCESLSN
                     NEIDLETKFKKISYADFLLQRKIIEISLSNQNDNLYKLNTVKETINFRTNLPTNKIKEA
                     ITGKILDGSYISKDGAVDFIXLKTNWQGELEINHLNSGIYDGLLGISSLLRNNADPNQI
                     EIINKLEQKALQEILNKNKNNYGFVNGVNAIISYYLTNLPHITSIDERLILQIFMDVNS
                     DIEKGKYSDKHFDILGGSAGLILTAVKFYQINPNYKILLKVAENLGDYIVNNMQTHNEF
                     VYWKAHDTLNLEDSLKGFVHGLSGQLLAFYKLKGILKTNKYDRVIHGIHKSEKMSMSEY
                     EYTDSWCKGFSGMGISRIKILESYQNEDIQSDLIFFKKQILSTLHENSDYCLCHGLMGK
                     LDFLLELENGGMLERNEKQIVMKVTNNFLNNFDIEDFNPHKIALFTGLGSTLYFLQRLE
                     DNKLNSILYFNA"
     gene            3103..5205
                     /gene="thuT"
     CDS             3103..5205
                     /codon_start=1
                     /gene="thuT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288:ABC
                     transporter related protein (Score: 186.9; E-value:
                     1.1e-56)"
                     /gene_kind="transport"
                     /note="ThuT"
                     /product="ABC transporter protein"
                     /protein_id="AHX39584.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 5.3e-34, bitscore:
                     105.6, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLRKKIKFIPQMTSFDCGPSCLAMIMHYYGSAVQASEIRNSKIIN
                     KKSAWSLLDIKKVSKSYGLSATAYRIENITDLQRIQKPMIAFWGFNHFVIIERVKANKF
                     YIVDPKAGPMIIDIRSFEEMFCDYILVIENKKDIQPNTKRREKHILLRYIDHFVSPSQI
                     VLLALLALLAQISILAFPFILKKFMDILNASKEVDVRYLLIILGSLIVVIGIKSITSII
                     KVNFQKEIDRKSTTKVFEHMFNTPIEKITTRHAGDLNVRIMSLESIRGYILEDLIEMII
                     SIVVIIPLLMYLLYTETFYTLILMFFIASSLFINISLGKFIYNANLVETYYLGEHRGKI
                     NESLKAMYYVKATGIFSRIKSVWQKDYNKYLDTVHKRALKQNALESFNSSLSTIFIIIM
                     LAVGFYNFSKGEGDLSNIFFFIAISSIIFSPVSTIIGSILNWNSVKPLLLRTLDILEEE
                     LEKDGSDTKVNILRGSIQFNNISFSFDDKKIIENLSLNIKNNESIAIVGESGSGKSTLA
                     NLLLKLHTPDKGNILIDDRDIKDWNSEELRKNVGLMTQDGVLFRGSLSENLSYFDINYD
                     PSKIQESIDKLNLSNYIQFQSINDILISEGGTNFSGGQRQRLAMLRLFMKEYPILILDE
                     PTNHLDRETANAVIENIFAIPSTKIIITHDENILERVDRVYELTDKKLEQREIFSKPQL
                     QTSGKG"
     gene            5278..6183
                     /gene="thuF"
     CDS             5278..6183
                     /codon_start=1
                     /gene="thuF"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 189.7; E-value:
                     1.1e-57)"
                     /gene_kind="transport"
                     /note="ThuF"
                     /product="ABC transporter protein"
                     /protein_id="AHX39585.1"
                     /transl_table=11
                     /translation="MKLAIQTKNLTKSFDNQSIVRDLNLSVPEGSLYAFIGPNGAGKTT
                     TMRMLLGLIPLDKGTILFNNKDIKDWKEELFENVGCFIDSPTYYPNLTAYENLAYVQKM
                     INKPLDEIDRVLKITGIYKAKDKKVKDFSLGMKQRLGISFAILNDPKILILDEPTNGLD
                     PEGVHEIRRLLLHLVKNEGKTIFISSHNLAEIEMIADHIALLHKGNLLYEGNLNSIITS
                     REYIVHVNDTTATERILNTKDIPYSIEKEGVFKLMIDQNSVPNLTRILIENDIDIFEVS
                     PKKLTLEEVFLSLTKQKELL"
     gene            6183..6908
                     /gene="thuE"
     CDS             6183..6908
                     /codon_start=1
                     /gene="thuE"
                     /note="ThuE"
                     /product="ABC transporter permease protein"
                     /protein_id="AHX39586.1"
                     /transl_table=11
                     /translation="MQFIFKSVNSEMRKIGGRKLLLFSLFFSLLSSFLCISYFIFNKNN
                     PDMFQSTNLIAITVNFNTLTLSIFSACMWYFIISSENKYGTWAIIYTKPISKFKLLVAK
                     NLLFILFYLIFNILNFSVMGVYIFINGYEISILYFLKMLLLCTILSLAIPYSQLLFHLI
                     IKKGILAASLSVIWIFFSVTYGLFPVVFTKTFPIFYALNIVSDVMMKETTILIYLASSS
                     IYILGIMFLAVSFKYYTYE"
     gene            6924..7622
                     /gene="thuG"
     CDS             6924..7622
                     /codon_start=1
                     /gene="thuG"
                     /note="ThuG"
                     /product="ABC transporter permease protein"
                     /protein_id="AHX39587.1"
                     /transl_table=11
                     /translation="MGILGEYKKSLHNGLYILLLVFLLIPISFQIFLTVSSQSKDGVFM
                     HIAILSLFCRLFLPIAIGLLVSFSFVNEFRRKGYMSAIYHGITLKTILSNKFLFCLMIT
                     FGYLICALVITSIPIVLTADNLGTVYQRLIFSILFMVISSLTIVNLHFLINIIFYKIPL
                     LSLLVGVIGGLGSFFISHTNYWILFPWSYPIMSLYIDSIEPKQLYIILIVYLLSLLSCI
                     FIIKKSMRWN"
ORIGIN
        1 atggaaactg aaaaatattt acaagtcgta gaagatgaag agattgaaca attagttggt
       61 ggagcaggac ctggttgggt tgaaacttta actaaagatt gtccttggaa tgtgcctgta
      121 gcatgtgtca cgattatggg acaaagaatt tgtaaaaaat gctactaata gttaaagaga
      181 gtaaggcgaa aggccttact ctctttaatg agaatgaatt tctaactgtt tctcctacct
      241 ataaaaagaa gaggttatta tgaaaaataa ttcacatgga attttgaata caatagataa
      301 tggatcacat tctttagtaa caaaagaata tctagaaaaa aattttgtta tctttttaga
      361 gggtttttca tgtaatgatg tcattaagga tgacgaaaac tataactttc aaaaaataat
      421 acaaacatat gtagtattct ttaaaaatat gataataaat aaaattgatt tttttggtaa
      481 ttttgagaag gtttctgaat cgataatgtt tgatttagaa aaaagactat accaagtatt
      541 aaggccatcc ttaattgtat acataaataa acttagggta gaaaatgctt taagaggaat
      601 ggataccaat gaagaatata agtattttac aaagaacttc tttcatttta aaaaatttta
      661 tatgaatttt tttaaaatat tacccgtatt aaacgaaaaa atttttacaa tcattgaaaa
      721 tgaaataaat tatatgattt ggttgcaagg tgctcttgaa aaagatcaaa aagaaattca
      781 taaaatgtcg ggttttaata ttatgcatgt agaaaaagtt attaacctag gtgactacca
      841 taataatgga aagagaacaa ttcttttaga agataaattt aataataagg taattttaaa
      901 accagtcgat ttgaaaaatt ctatactgta tcatagttta attaatcaac tgaataaaga
      961 tttaaatacg gatatcttta ttccgcaagt agttaataaa agaaattatg gatatatgga
     1021 atacattaat catgaatcat gtgaatctaa tgagcaaatt aaagattatt attataattt
     1081 aggtgtagtg ctttcggtaa tgtatttgat taatggaagt gacatacata atgaaaatat
     1141 catagctaaa caatcctcac cagtcataat tgattttgag acattaggaa gtacattaaa
     1201 tccaagtgtt actgaagaaa ccagtagttt tatattatct aattcagtat taaattctag
     1261 aatgctacct ataaggttta gtggaggtag ggaggtaata agagattaca gtgcaattgg
     1321 gagagtaatg aagtctttgg taaaaacaat taaaataaaa aatgaattta catcgaatcc
     1381 aattgaaata agagaagaga caatagtaga agatacggtt cagaatttac cgtttcttaa
     1441 cgatgatata tacgagtatg ataattatat agatgatatt attaaaggtt ttgagaatgc
     1501 atataactca gttttaaaaa acaaagagga gtatatgtgt atattaaaaa atagtgtttc
     1561 taaatggaat tatcgaaaag tatatcgaaa tacaaaagtc tatacccttt tattagatag
     1621 actaaatgtt ccggatttat tagagaataa attaaagact ataaaatatt tgcggaatat
     1681 tcttgaaaaa aacatatact tggctactag gaaagatatt attcaaaaag agatagaggc
     1741 actattatcc tatgatgttc catatttctt taataagtat gaatgggagt cttgcgaaag
     1801 tttatcaaat aatgaaatag atctagaaac aaaatttaaa aaaatttcat atgctgactt
     1861 tttattacaa agaaaaatta tagaaatatc cctttctaat caaaatgaca atctatataa
     1921 gctgaataca gtaaaggaaa caattaattt tcgaactaat ttacctacaa ataaaataaa
     1981 ggaagcaatt acagggaaaa ttcttgatgg cagttatata tcaaaagacg gtgctgtaga
     2041 ttttattgna ttaaaaacaa attggcaagg ggagcttgag ataaaccatt tgaatagtgg
     2101 aatatatgat ggattattgg gaataagttc attattgaga aataatgctg atcctaatca
     2161 gattgaaatt ataaataaac ttgaacaaaa agccctgcaa gaaatattaa acaaaaataa
     2221 aaataattat ggttttgtaa atggtgttaa tgcaattatt tcttactatt taacaaattt
     2281 accacatata acatctatcg atgaaagatt gatattgcaa atatttatgg atgtcaactc
     2341 tgatattgaa aaaggtaaat atagcgataa gcattttgat atattagggg gttcagctgg
     2401 tctaatttta acggcagtta agttctatca aattaatcct aattataaaa tattgcttaa
     2461 agtggctgaa aatctcggtg actacattgt caataatatg caaacacata atgaatttgt
     2521 atattggaaa gctcacgata ctctaaattt agaggattca ttaaaggggt ttgttcatgg
     2581 attatcagga caacttctcg cattttataa gttaaaaggc atattaaaaa caaataagta
     2641 cgatagagta atacatggta tacataaaag tgagaaaatg agtatgagtg aatatgagta
     2701 tacagatagc tggtgtaaag gatttagtgg aatgggaatc tcgagaatta aaattttaga
     2761 atcgtatcaa aatgaggata tacaatcaga tttaattttt tttaaaaaac aaatcttaag
     2821 tacattacat gaaaatagtg attattgttt atgtcatggt ttaatgggga aactagactt
     2881 tttattggag ctagaaaatg gaggtatgct cgagcgcaat gaaaaacaaa ttgttatgaa
     2941 agtaacaaat aactttttga ataattttga tatagaagat tttaatcctc ataaaatcgc
     3001 attatttaca gggctaggaa gtactttgta ttttttacaa aggttagagg ataataaact
     3061 aaatagcatt ttatacttta atgcctagaa taggtgataa atatgttaag gaagaaaatt
     3121 aaatttatac cgcaaatgac atcttttgat tgtgggccta gttgtctagc aatgattatg
     3181 cattattatg gttcagcagt acaagcttcg gaaattagaa atagtaaaat tatcaataaa
     3241 aaatcggcat ggagtttgtt ggatattaaa aaagttagta aatcgtatgg actttctgca
     3301 actgcatata ggatagaaaa tataactgat ttacaaagga tacaaaagcc gatgatagca
     3361 ttttggggct ttaatcactt tgtaattatt gaaagggtta aagcaaataa attttatatt
     3421 gtagatccta aggcagggcc gatgataatt gatataagaa gttttgagga aatgttctgt
     3481 gactatatcc tagtaataga aaacaaaaaa gatatacagc caaatactaa aaggcgtgaa
     3541 aaacatatat tacttagata tattgatcat tttgtatctc ctagccagat tgttttatta
     3601 gcactattag cattgttagc acaaatctca attcttgctt tcccctttat tttgaaaaag
     3661 tttatggata ttttgaatgc ttcaaaggaa gtggatgtaa gatacctttt aattattttg
     3721 ggaagtttaa tcgttgttat agggataaaa tctataactt ctattataaa agtaaacttt
     3781 cagaaagaaa ttgatagaaa aagtacaaca aaagtttttg agcatatgtt taataccccc
     3841 atagaaaaaa ttactacacg tcatgcaggg gatttaaatg taagaatcat gagcctagaa
     3901 tcaatacgag gatatatttt agaggatcta attgaaatga ttatatcaat agtagttatc
     3961 attccattat tgatgtatct attatatact gagacttttt atactttaat attgatgttt
     4021 tttattgcta gttccctatt tatcaatatt tccttaggga aattcatata taatgctaat
     4081 ttagtcgaaa cgtattacct aggagaacat agagggaaaa ttaatgaatc attaaaagcg
     4141 atgtattatg taaaggctac agggattttt tctagaatta aatctgtatg gcaaaaagat
     4201 tataataaat acttagatac agtacataaa cgagcgctaa aacaaaatgc tttagaatca
     4261 tttaatagtt ccctatcaac aatatttatt atcataatgc ttgctgtagg attttataat
     4321 tttagtaaag gagaagggga tttaagtaat attttcttct ttattgctat atctagtatt
     4381 atattttctc cggtttcaac aattatagga tcaattttaa attggaattc agttaaacca
     4441 cttttattaa gaactttaga tatcttggaa gaggaactag aaaaagatgg atcggatact
     4501 aaagtaaaca ttcttagagg atcgattcaa tttaataata tttcgtttag ttttgatgac
     4561 aaaaaaataa tagaaaactt aagtttgaac attaaaaata acgaaagtat tgctattgtt
     4621 ggagaaagtg gcagtggtaa gtccacttta gctaacttat tattaaaatt gcatactccc
     4681 gataagggta atatattaat agacgataga gatattaaag attggaacag tgaggaacta
     4741 agaaaaaatg taggactaat gactcaggat ggagttttat ttagaggaag tttatctgag
     4801 aatttatctt actttgatat aaattatgat ccttcaaaaa tccaggagtc tattgataaa
     4861 ttgaatctga gtaattatat tcaatttcaa agtataaatg atattttaat ctcagaggga
     4921 ggaacaaatt tctccggtgg ccaaagacaa aggttggcta tgttacgatt gttcatgaaa
     4981 gaatatccga ttcttatttt ggatgaacca actaaccatt tagataggga aacggcaaat
     5041 gctgttattg aaaatatatt tgccatacca tccactaaga ttattatcac tcatgatgaa
     5101 aatattttag agcgtgtaga tagagtttat gaacttactg ataaaaaatt agaacaacga
     5161 gaaatatttt ctaaacccca attgcaaaca tcaggtaaag gataaataga atatctaata
     5221 gatcaagaat ttaatagata cttactttta tttgagaata agaaagtgag gaacaaaatg
     5281 aaattagcaa tccaaactaa aaatttaaca aagtcttttg acaatcaatc cattgtaagg
     5341 gatttaaact tatctgttcc tgaaggaagt ttatatgctt ttattggacc aaatggtgca
     5401 ggaaaaacaa caacaatgcg aatgctatta ggattaattc cattagataa aggaacgata
     5461 ttatttaata ataaagatat caaagattgg aaagaagaat tatttgaaaa tgtgggttgt
     5521 tttattgatt cacctaccta ctatcctaat ttgacagcat atgaaaattt agcctatgta
     5581 caaaaaatga ttaataagcc tttagatgaa atagacagag tattaaaaat tacaggaata
     5641 tataaagcaa aagataaaaa agtgaaagat ttttctttag gaatgaaaca aaggctaggt
     5701 atttcctttg ctattcttaa tgatcctaaa atactaattc ttgatgagcc gacaaatgga
     5761 ttagacccag agggagtaca cgagataagg cgcttattac ttcatttagt aaaaaatgag
     5821 gggaagacaa tatttatttc tagtcataat ttagctgaaa ttgaaatgat agcagatcac
     5881 atagcattac tacataaggg taacttactc tatgaaggga atttaaacag tattattact
     5941 tctagagaat atattgttca tgttaatgac actactgcca cagaaaggat tttaaataca
     6001 aaggatatac cttatagcat agaaaaagaa ggggtattca aactaatgat agatcaaaat
     6061 agtgttccta atttaaccag aatattaatt gaaaatgata tagatatttt tgaagttagc
     6121 cctaaaaagc taacattaga ggaagtattt ttatctttaa ctaaacaaaa ggagttacta
     6181 taatgcaatt tatctttaaa agtgtgaata gtgagatgag gaaaattggt gggagaaaat
     6241 tattgttatt ttctttgttt ttttcattat tatcaagctt tttgtgtata agttatttta
     6301 tttttaacaa gaataatccc gatatgtttc aatctaccaa tcttatagca attacagtaa
     6361 actttaatac actaacatta agcatttttt ctgcttgtat gtggtatttt atcatttctt
     6421 ctgaaaataa atatggtact tgggctatta tttatacaaa acccatttca aaattcaagt
     6481 tattggtagc taaaaattta ttgtttatac tgttttattt aatctttaat attttaaatt
     6541 tttcagttat gggtgtctat atttttataa atggatatga aatatctata ctgtattttt
     6601 taaaaatgtt gttactttgt accattttat ctttagcaat accatattct caattgcttt
     6661 ttcatttaat tataaaaaag ggaattcttg cggcgtcctt gtcagttatt tggatttttt
     6721 ttagcgtaac ttatggatta tttcctgtag tattcacaaa gacatttcca atattttatg
     6781 cattgaatat agtaagtgat gtaatgatga aagaaacaac tattttaata tatcttgcaa
     6841 gttcttcaat ctatatttta ggaattatgt ttttagctgt gagttttaaa tattatactt
     6901 atgaataata aagggtgaaa taagtgggta tattaggtga atataaaaaa agtcttcata
     6961 atggattgta tatcctgtta ctagtatttc tgttgatacc aattagtttt caaatctttc
     7021 taactgtatc atctcaatca aaagatggcg ttttcatgca tatagcgatt ctaagtcttt
     7081 tttgtagatt atttttacct attgcaatag gtctattagt gtcattttct tttgtaaacg
     7141 aattcagaag aaaaggatat atgtctgcta tttatcatgg aatcacttta aaaactatat
     7201 taagtaataa gtttttgttt tgcttaatga ttacctttgg ttatttaata tgtgctttag
     7261 tcataactag tatacctata gtattaacgg ctgataatct tgggactgtt tatcaaaggt
     7321 taattttttc tatacttttt atggtgatta gctctttaac tattgtgaat ctacattttt
     7381 taataaatat aatattttat aaaatcccgt tactttcatt attagtaggg gttataggtg
     7441 gactgggtag tttttttatc tctcatacaa actattggat actgtttcct tggtcatatc
     7501 caattatgag tctttatatc gatagcattg aaccaaaaca gttgtatatt attcttattg
     7561 tatatctatt atcattacta agttgtatat ttattattaa aaaatcaatg cgatggaatt
     7621 aa
//
