LOCUS       BGC0000633             14304 bp    DNA     linear   BCT 11-OCT-2001
DEFINITION  Streptomyces avermitilis carotenoid biosynthetic gene cluster.
ACCESSION   BGC0000633
VERSION     BGC0000633
KEYWORDS    .
SOURCE      Streptomyces avermitilis
  ORGANISM  Streptomyces avermitilis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 13:57:38
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 14304
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..363
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="BAB69138.1"
                     /transl_table=11
                     /translation="MPHPAERSRHGRGWFEKLAEAASNLTSSPVFYCFCLLLVFAFIVA
                     HAVGLELTWQLLIGDIMTAVNLLLLALLKNSERRAEHAIQHKLDSIAAALLEQREGHSR
                     QAHEELRKAIGIEDEF"
     source          1..14304
                     /db_xref="taxon:33903"
                     /mol_type="genomic DNA"
                     /note="Actinobacteridae"
                     /organism="Streptomyces avermitilis"
     protocluster    1..14304
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="True"
                     /core_location="[3452:8763]"
                     /cutoff="20000"
                     /detection_rule="(Terpene_synth or Terpene_synth_C or
                     phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7
                     or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      3453..8763
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(Terpene_synth or Terpene_synth_C or
                     phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7
                     or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
     cand_cluster    1..14304
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Terpene_synth or Terpene_synth_C or
                     phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7
                     or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..14304
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000633"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..14304
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="terpene"
                     /region_number="1"
                     /rules="(Terpene_synth or Terpene_synth_C or phytoene_synt
                     or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps
                     or fung_ggpps2 or trichodiene_synth or TRI5)"
                     /subregion_numbers="1"
                     /tool="antismash"
     CDS             complement(419..967)
                     /codon_start=1
                     /product="integral membrane protein"
                     /protein_id="BAB69139.1"
                     /transl_table=11
                     /translation="MSLIERRQRSSDEARTSQEGRASDSGTRADEGESEQERVNRRWQE
                     ILQETRVAQTGVQILFGFLLSVAFTPLFRELGTFDRGVYVAAVLLGAAATGALIAPVSI
                     HRFLSGQRMKDELVAAAGRLMMGGIVLLALTIGCTVLLILHAVLPSSLAEILAGAVMLW
                     FAFCWYLLPWILRSRADKG"
     gene            complement(1122..2624)
                     /gene="crtU"
     CDS             complement(1122..2624)
                     /codon_start=1
                     /gene="crtU"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_kind="biosynthetic-additional"
                     /product="phytoene desaturase"
                     /protein_id="BAB69140.1"
                     /sec_met_domain="DAO (E-value: 7.7e-15, bitscore: 44.8,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLPPRPGATRFNGARPPTTAVVGGGIAGLAAATALAERGVRVTLY
                     ERQPDLGGRLSGRPVGLRDGSTVTMSRGFHAFFRQYYNLRGLLRRTDPRLERLTALPDY
                     PLRHSAGLHDSFRRVPRTPPWSALGFVALSPTFGLRDLIRMNPVAALPLLDVSVPDVYT
                     RLDAVSAHDFLRSIRFPEAAHHLAFEVFSRSFFADPRELSAAEMALMFHIYFLGSSEGL
                     LFDVPDEPFPTALWDPLAGYLLRHRVEVRTSTSVESVAPTRDGGFAVATPEETGRHDTV
                     VLALDAGGLRSLAGRSPTLADAPWRERMARLRNAPPFLVSRLWLDRPVARDRPGFLGTS
                     GYGTLDNVSVLERWEGEAARWSARTGGSVVELHAYALPEDAVREVEQKRMLEQLCRVYP
                     ETRAATVVDERHEWRADCPLFPVGGYHDRPTVRTPEPGLVVAGDLVRTGLPVALMERAA
                     TSGFLAANALLERWGVRGQTLWTVPDGGRSAVLRRVARWGSA"
     gene            complement(2678..3409)
                     /gene="crtT"
     CDS             complement(2678..3409)
                     /codon_start=1
                     /gene="crtT"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089:methyltransferase (Score: 74.4; E-value:
                     1.7e-22)"
                     /gene_kind="biosynthetic-additional"
                     /product="methyltransferase"
                     /protein_id="BAB69141.1"
                     /transl_table=11
                     /translation="MTLLRDADLAAAFDRAARSYDTLVAANPGYHAHLRRSARRLGLPH
                     GGSGTRVLDLGCGTGASTAALAAVLPGAEITAVDASAGMLERAAAKPWPDRVTFVHAPA
                     ERLTEAGVEGPFDAVFAAYLFRNAAAPDSVLTAVHDVLAPQGRLAVHDYTLSGRARDRT
                     VWTVVCGGIVLPVATALGDGRLYRHLWRSVVEFDTADEFAGRVRAAGFDCVRVLPLPGW
                     QTGITHTVVGRRAGTDTATGR"
     gene            complement(3453..4799)
                     /gene="crtY"
     CDS             complement(3453..4799)
                     /codon_start=1
                     /gene="crtY"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: Lycopene_cycl"
                     /gene_kind="biosynthetic"
                     /product="lycopene cyclase"
                     /protein_id="BAB69142.1"
                     /sec_met_domain="Lycopene_cycl (E-value: 2.3e-114,
                     bitscore: 371.9, seeds: 20, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGGAAAPGRPGRSAAGVSRTADRTARPASGPAHEAECFRQGAWAR
                     AEGGNAVLEADVAIVGAGAAGLSLAHRLACPVRGAPRISVVLLEAPPGPLRPPRRTWCF
                     WERGPGRYDAAVTASWQRLRVRAPGGRPIEGDITPLRYKMIRSDDFEALMAHDLALSGG
                     VQRVEATVEAVEGVPGGAEVYAYTAAGRPLPVRARWVFDSRPLGSLPAARTTLLQHFHG
                     WFVRSDLPVFDPGTVELMDFRTPQPPRGLSFGYVLPTGRRQALVEYTEFSPAVLPRSAY
                     EAALRHYTRDILHLPGLEIVSTETGVIPMTDAPFARQTAASVFRIGAAGGATRPSTGYT
                     FAAVQRQTRAVAAALCRGRRPLPPPAHSARSRAMDAVLLRALDSGRIGGAAFFARLFSR
                     VPMERLLRFLDGCTHLHEDLSIGVHTPVLPMLRSAAELPYLPRRPFPGP"
     gene            5061..6200
                     /gene="crtE"
     CDS             5061..6200
                     /codon_start=1
                     /gene="crtE"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182:Polyprenyl synthetase (Score: 245.1; E-value:
                     1.4e-74)"
                     /gene_kind="biosynthetic-additional"
                     /product="geranylgeranyl pyrophosphase synthase"
                     /protein_id="BAB69143.1"
                     /transl_table=11
                     /translation="MGRLLDRFLAERVARAAALDPVFAHDIAERVARFTLDGGKRTRSQ
                     FVWWAHRACGGPAAGAEAALRVGAALELIQTCALVHDDVMDGSRLRRGRPALHTDVSAQ
                     YADAVLPAPGTRFGEAAAILAGDLALAWADDVVADTDLAPDPGRRVREVWSCLRMEMVA
                     GQYLDIQGQATSSHSEARAMRAACLKSALYSVQRPLELGAALAGADTATTQALCSAGRC
                     VGLAFQLRDDLHDVFEEPRRTGKPSGGDIRAGKPTYLVAVARARAEAAGDRGALAVLRR
                     SLGCADLSDADLARVRDVLVTTGARDTVEAEIDRLVAQGLRHMGAASLAPEGRRHLDAL
                     LRSAAGAPSVRRQAPDASRSENGMPVSLLLTATAEGASR"
     gene            6197..7738
                     /gene="crtI-1"
     CDS             6197..7738
                     /codon_start=1
                     /gene="crtI-1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222:dehydrogenase (Score: 482.4; E-value: 3.2e-146)"
                     /gene_kind="biosynthetic-additional"
                     /product="phytoene synthase"
                     /protein_id="BAB69144.1"
                     /sec_met_domain="DAO (E-value: 3.9e-21, bitscore: 65.5,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNRTLRGRTDHVVVVGAGLAGLSAALHLLGAGRRVTVVERDLLPG
                     GRAGRLVRGGYLIDTGPTVLTMPDLADEAFAAVGDSLRDRVDLIPLHPAYRARFADGST
                     LDVHTGAEAMEAEIERFAGAREAAGYRQLRDWLERLYRAQMRRFIDSNFDSPLQLLTPD
                     LARLAALGGFGRLDARIGRHLRDERLRRVFSFQALYAGVPPARALAAYAVIAYMDTVAG
                     VYFPRGGMHALPRAMADAAAQAGAELRFGQDVTRLERAGDRITAVVTAYDRIPCDAVVL
                     TPDLPVTYRLLGRAPRRPLRIRNSPSAVVLHAGTDRTWPGLAHHTLSFGAAWGRTFDEL
                     TRTGGLMSDPSLLITRPTATDPGLAPPGRHLHYILAPCPNTDIGPSAAAWGDLAPRYRD
                     SLLTELEARGLDGIGSAVEEECLVTPADWAAQGHAAGTPFSAAHTFAQTGPFRPRNLVR
                     GTANAVLAGCGTTPGVGVPTVLLSGKLAAARVTGGTRRTPRRMPASRTASGRTLS"
     gene            7735..8763
                     /gene="crtI-2"
     CDS             7735..8763
                     /codon_start=1
                     /gene="crtI-2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: phytoene_synt"
                     /gene_kind="biosynthetic"
                     /product="phytoene synthase"
                     /protein_id="BAB69145.1"
                     /sec_met_domain="phytoene_synt (E-value: 1.5e-120,
                     bitscore: 390.9, seeds: 8, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTDRELDAAGITDPSLRKAYTHCRRLNARHGKTYFLATRLLPVER
                     RPAVHALYGFARWADDIVDSLDTTVGPAVRSAALGRLQESLRTGLREGHSTEPVVLALA
                     ETARRYAIDHQHFSDFMTAMRSDLEVTGYETYADLRGYMHGSAAVIGLQMLPVLGTVVP
                     REEAAPHAAALGVAFQLTNFLRDVGEDLDRGRVYLPADLLRAHGVDRDLLRWSRDTGRR
                     DRRITRALKAVEGLTRGVYREAAPGLAMLDPVARPCIRTAFVLYGGILDAIADDGYAVV
                     HRRAVVPRRRRAAVALDGLVRLGVARLGSRADARLPTLPDPWRLPALVPRSSPRPAHEE
                     VA"
     gene            8760..9839
                     /gene="crtV"
     CDS             8760..9839
                     /codon_start=1
                     /gene="crtV"
                     /product="methyltransferase"
                     /protein_id="BAB69146.1"
                     /transl_table=11
                     /translation="MTPDRTDRPARRRRYPLSLRRHPIRWEDQRPTWRDARPAVIAEAL
                     KRAQARPSGNWYVVGATRDVREDRPLARTVAGLEVVVWRDGEGRLVAGPGVCPHLGAPL
                     RDSPVRCGTLMCRWHGLALSGTPFAGWEPLPVHDDGVLVWVRLDEVGGERPLEAPVVPA
                     RPALSGAVSAVYVGVGICEPEDVVANRLDPWHGAWFHPYSFVDLTVVTPGETVAPEEGE
                     TEGVETGGDEAEGAETEDGFTVDVSFKIAGRLVVPVRAVFTAPEPRTVVMRIAEGEGRG
                     SVVETHATPLGPDDRGRPRTAVVEAVLASSDRRGFAVARAMAPVLRPLMRAAAGRLWRD
                     DLAYAERRRHLRSTGRFPG"
     CDS             complement(10060..12159)
                     /codon_start=1
                     /product="secreted protein"
                     /protein_id="BAB69147.1"
                     /transl_table=11
                     /translation="MSGEHTLARRTRTRRLLGTIGVTALATGAALVTAPPTNAQTPAAA
                     ATAAVTVQPDPSYRADRFEGWGTSLVWFANATGDYPAEIREKLAELLFDDDGLALNIAR
                     YNIGGGNAPDVKDYLRAGGAVEGWWNAPAGTTREDTDWWSADDAADWNPNADATQRWWV
                     DRIKNDIDHWETFSNSPPWFMTASGYVSGGFNSSEDQLKADSVDDFAAYLAGATKRLEK
                     AHGIKVDTLDPFNEPNTGYWGTRLGSDGRPVGGRQEGAHIGPELQQKVIRALAPALKKA
                     KTGTKISAMDETNPNLFAQNWNSYPQDVRDLVGQMNVHTYGTGGRTSVRDLAKAADKPL
                     WMSEVEGDWGDGQSFTDMRPGLGLAQQMVDDLRELEPKAWVFWQPVEDYDNMKPGGESA
                     KGGNWGSIQLPFSCTSADTLATCPVHTNTKFDTARNFTHYIKPGDRLIRTDDTSSAAAV
                     STKGNAATVVHVNKTTSARTVTIDLSKFGHITRDATVTPVVTSAAGKLERQAPVEVTGS
                     RAAVTVPAQSVTSFLVKGVSGVAKDAALLCKGHTYQLTGVQSGKALTVADDGTQLVIRT
                     AGTDAAGTGQQWQVRQISGDTGNRQRYVFTNPAAGKRLAVRDGAPVLEADRGHRDKATQ
                     WIMSTTGDGTWTLVNAATGRLLEVGGQATTDGAAVTTWTPNSGSNQRWSVTDVTDQNGS
                     RSSGL"
     CDS             complement(12242..14284)
                     /codon_start=1
                     /product="beta-galactosidase"
                     /protein_id="BAB69148.1"
                     /transl_table=11
                     /translation="MISTLLSQLNGPDGDRTPRLAYGADYNPEQWPREVWEEDIRLMRE
                     AGVNIVSVGIFSWARIQPAQGQWDFAWLDDVMDLLHAGGIGVDLATATASPPPWLATAH
                     PEILPVTASGETVWPGARQHWRPTSPVFRTHALRLVRKLAERYADHPALVAWHVSNELG
                     CHNIYDYSDDAARAFRDWLRARYTTLDALNHAWGTAFWSQYYSDWDQILPPRLAASHPN
                     PTQQLDFKRFSSDALKAYLRAERDLLREITPEVPVTTNFMVMGRTKGMNYADWAQEIDF
                     VSNDHYVSPGPQDRDELSFSANLVSGIAGGRPWFLMEHSTSAVNWQPVNLAKRPGDLAR
                     DSLLHVAHGADAVCFFQWRQSAAGAEKYHSAMVPHAGPDSEVFRAVTALGGTLRTLAPV
                     AGTEREPARVGIVFDWESWWASEQDSHPTSLLDYRREALDWYSALLALGIRADVVTPQA
                     DLDRYQLLITPVLHVVPAALAKELTRYAENGGHLVTTYFSGVVDENDHIWLGGYPGALR
                     DLLGIRVEEFGPLLDGDTVALDDAATGTLWTDRITVTDPEVEVLARYRTGTYAGHPAVT
                     RRAVGRGSAAYVSTRLGPDGLTALLPKLLGPAAVGSELPDGVGGDVELIVRRGAGSRYL
                     FLVNRTDGTVPLTGLAGELLIGRAEDDGTVVLSPRDVAVLRQPTR"
ORIGIN
        1 gtgccccatc cggcagaacg gtcccgccac gggcggggct ggttcgaaaa gctggccgag
       61 gcggcctcca atctcaccag ttctccggtc ttctactgtt tctgtctgct cctggtgttc
      121 gcgttcatcg tggcgcacgc agtcggtctc gagctgacgt ggcagctgct gatcggggac
      181 atcatgaccg ccgtcaacct gctgctgctg gccctgctga agaactccga gcgacgcgcc
      241 gagcatgcca tccagcacaa actcgacagc atcgccgccg cgctgctcga acagcgcgag
      301 ggccacagtc gccaggcgca cgaagagctc cgtaaagcga tcggcatcga ggacgaattc
      361 tgaccgcacc gccgggccgg gctgcggtcc acgcggactg gcagtgccgc gccctgatct
      421 accccttgtc ggcgcgcgac cgcagtatcc agggcagcag ataccagcag aacgcgaacc
      481 acagcatcac ggcaccggcc aggatctcgg cgagtgaact gggcaggacc gcgtgcagga
      541 tgagcaggac ggtgcatccg atggtgagcg cgagcagtac gatgccgccc atcatcaagc
      601 gcccggcggc cgcgacgagt tcatccttca tccgctggcc ggagaggaaa cggtggatcg
      661 acacgggcgc tatgagcgcg ccggtcgcag cggccccgag gaggacggcc gcgacataga
      721 cgccccggtc gaacgttccc agctcccgga acagcggggt gaaggcgaca ctgagcagga
      781 agccgaacag gatctgcacc ccggtctgcg cgacacgggt ttcctgaagg atctcctgcc
      841 accgccggtt gacccgctcc tgctcggatt cgccctcgtc cgcacgtgta ccggagtcgc
      901 tcgcacgccc ctcctgagaa gtgcgcgcct cgtcggacga ccgctgtctg cgctcgatca
      961 aactcacgga tcttcctcca caatgggcca tggccgctgc ccgtccggac cggcacgaca
     1021 atcttcgcct cacacacgtg cccgcattcg ggcccgtcag acggggttgc gcagggcctc
     1081 gccggcgaaa gaccggatga cccacggagc cggacgccgg gctatgcgct cccccaccgc
     1141 gccacccgtc gcagcacagc gctacgccct ccgtccggta ccgtccacag cgtctgaccg
     1201 cgcacgcccc aacgctccaa cagggcgttc gcggcgagga aaccgctggt cgcggcgcgt
     1261 tccatcagcg ctacgggcag gccggtgcgc accaggtcac cggccaccac cagtcccggt
     1321 tcaggggtgc gtacggtcgg tcggtcgtgg tatccgccga cggggaacag cgggcagtcg
     1381 gcccgccatt cgtgccgctc gtcgacgacc gtcgccgccc gcgtctccgg gtacacccgg
     1441 cacagctgtt cgagcatgcg cttctgctcc acctcgcgga ccgcgtcctc cggcagcgcg
     1501 tacgcgtgga gctccaccac ggatccgccg gtacgagccg accagcgggc ggcctcgccc
     1561 tcccagcgct ccagcacact gacgttgtcc agggtcccgt atccgctggt gcccaggaaa
     1621 ccgggccggt cccgggcgac gggccggtcg agccacaggc gcgacaccag gaacggcgga
     1681 gcgttgcgca gccgcgccat ccgctcgcgc cacggggcgt cggccagggt cggggagcgg
     1741 ccggccaggg accgcaggcc ccccgcgtcc agggccagca cgaccgtgtc atgacgcccc
     1801 gtctcctccg gcgtggcgac ggcgaacccg ccgtccctcg tgggggcgac gctctcgacc
     1861 gacgtcgagg tgcgcacctc gacgcggtgc cgcagcagat agccggcgag ggggtcccac
     1921 agggcggtcg ggaacggttc gtccggcacg tcgaagagca gtccttcgga ggaacccagg
     1981 aagtagatgt ggaacatcag cgccatctcg gccgcggaca gctccctggg gtccgcgaag
     2041 aagctgcggg agaacacctc gaacgccaga tggtgcgcgg cctcggggaa acggatcgac
     2101 cgcaggaagt cgtgggcgct gacggcgtcc agacgcgtgt acacatcggg gacgctgacg
     2161 tcgagcaggg gcagcgccgc caccgggttc atgcggatca ggtcccgaag gccgaacgtg
     2221 gggctgagcg cgacgaatcc gagcgcgctc cacggcgggg tacgcgggac ccggcggaag
     2281 ctgtcgtgca gcccggcact gtgccggagc ggatagtcgg gcagagccgt caggcgctcc
     2341 agccgcggat cggtccggcg cagcagcccg cgcaggttgt agtactggcg gaagaaggca
     2401 tggaaaccgc ggctcatcgt caccgtggag ccgtcccgaa ggccgaccgg ccggccggag
     2461 aggcggccgc cgaggtccgg ctgtcgttcg tagagggtga cgcgtactcc ccgttcggcc
     2521 aacgcggtcg cggccgccag tccggcgatg ccgccgccca cgacggcggt cgtggggggt
     2581 cgtgctccgt tgaaccgcgt cgccccggga cgagggggca gcatccgggc gcgccggtcc
     2641 ctgccgcgac gtgcggcggc cggccgccgg gagagggtca tcggccggtt gccgtgtcgg
     2701 tgcccgcgcg cctgccgacg acggtgtggg tgatgcccgt ctgccacccc ggcagcggca
     2761 gcacccgtac gcagtcgaac ccggccgccc ggacgcggcc ggcgaactcg tccgccgtgt
     2821 cgaactcgac gacgctgcgc cacaggtggc ggtacagccg tccgtcgccg agggccgtgg
     2881 ccacgggcag cacgatgcct ccgcatacga ccgtccacac cgttcggtcc ctggcgcgac
     2941 cgctgagcgt gtagtcgtgg accgccagcc gtccctgcgg tgccagcacg tcgtgcaccg
     3001 cggtgaggac gctgtcgggg gcggccgcgt tgcggaagag gtaggcggcg aacaccgcgt
     3061 cgaacggtcc ctcgaccccc gcctcggtca ggcgttccgc cggggcgtgc acaaaggtca
     3121 cgcggtccgg ccagggcttg gcggccgccc gttccagcat gccggcggag gcgtccaccg
     3181 cggtgatctc ggcgcccggc aggaccgcgg cgagtgcggc ggtcgaggca cccgtaccgc
     3241 agccgagatc gagcacgcgc gttccgctcc cgccgtgcgg cagaccgagg cgtcgggcgg
     3301 agcgccgaag gtgggcgtgg tagccgggat tggccgccac cagagtgtcg tagctgcggg
     3361 cggcgcggtc gaacgcggcg gccagatccg cgtcgcgaag cagggtcata cggtctcctc
     3421 cggcgggcgt gcgggccgtg tccgcgtctc ggtcacggcc cgggaaaggg acggcggggc
     3481 aggtacggca gctccgcggc ggagcggagc atcgggagta cgggggtgtg caccccgatg
     3541 gagaggtcct cgtggaggtg cgtgcagccg tcgaggaagc gcagcagccg ctccatgggc
     3601 acccgtgaga agagccgcgc gaagaaggcc gcaccgccga tccggccgct gtccagggcg
     3661 cgcagcagca cggcgtccat ggcgcgggag cgggcggaat gggccggcgg cggcagcggg
     3721 cggcggccgc ggcacagggc ggccgcgacg gcgcgcgtct ggcgctgcac ggcggcgaag
     3781 gtgtatcccg tcgacgggcg tgtcgcacct cccgcggcgc cgatccggaa gaccgacgcc
     3841 gcggtctgcc gggcgaacgg cgcgtcggtc atgggaatca cgcccgtctc ggtcgagacg
     3901 atctccaggc ccggcaggtg gaggatgtct cgcgtgtagt gccgcagggc cgcctcgtag
     3961 gcggaccgcg gcaggacggc gggcgagaac tccgtgtact ccaccagcgc ctgccggcgg
     4021 ccggtgggca gcacgtatcc gaacgacagt cctcgcgggg gctggggtgt gcggaagtcc
     4081 atgagctcca cggtgccggg gtcgaagacc ggcagatcgg agcggacgaa ccagccgtgg
     4141 aaatgctgca acagcgtggt gcgggccgcc gggaggctgc cgagcgggcg cgagtcgaag
     4201 acccagcggg cacgcaccgg cagcgggcgc ccggcggcgg tgtacgcata cacctcggca
     4261 cccccgggca cgccctccac cgcttcgacc gtcgcctcga cgcgctggac gcccccgctc
     4321 agcgcgaggt cgtgcgccat cagagcctcg aagtcgtccg accggatcat cttgtaccgc
     4381 agcggcgtga tgtcgccctc gatcggccgc ccgccggggg cgcggacccg caggcgctgc
     4441 catgacgcgg tgacagcggc gtcgtaccgg ccggggcccc gctcccagaa gcaccacgtg
     4501 cgcctcggcg gcctcagggg gcccggcggg gcctccagca ggaccaccga gatgcggggc
     4561 gccccgcgga cagggcatgc caggcggtgg gccagggaca gtcccgcggc tcccgcgccc
     4621 acgatcgcga cgtccgcctc cagcacggcg ttccctcctt ccgcgcgagc ccatgcgcct
     4681 tgtcggaagc attccgcttc gtgggccggt ccggatgcag ggcgcgcggt cctgtcggcc
     4741 gtgcggctga ctccggcggc cgaacgaccc ggccggcccg gtgcggcggc gccgcccacc
     4801 accccgtcgg cgccgcccac catcccgtcg gcgccgccca ccaccccgtc ccggccgtct
     4861 cagcgctccg cattccctcc aatggcgcat ccgtgtgcgc cgccgacgcg gaatgcgttc
     4921 agacaaggcc agctccgatc acgacgagag gaaccgggat gcgccgcagc aaggcgaccg
     4981 atcaccaggc ggccggtccg tcaccgggcc cgcgcgacgc gttccggccg gcctccgtcg
     5041 acgaggacgt accggccgcc gtgggccgac ttctcgaccg cttcctggcg gagcgcgtgg
     5101 cgcgggcggc ggccctcgac ccggtgttcg cccacgacat cgccgagcgc gtggcgcggt
     5161 tcaccctgga cggcggcaag cgcacccggt cccagttcgt gtggtgggcg caccgggcgt
     5221 gcggcggacc ggccgccggg gcggaggcgg ccctgcgggt cggtgccgcc ctcgaactca
     5281 tccagacgtg cgcgctggtg cacgacgacg tgatggacgg ctcccggctg cgccgcggcc
     5341 ggcccgcgct gcacacggac gtgagtgccc agtacgccga cgccgtcctg cccgccccgg
     5401 gcacacgctt cggtgaggcg gccgcgatcc tcgccggaga cctggctctc gcctgggcgg
     5461 acgacgtggt ggcggacacc gacctggcac cggacccggg gcgcagggtg cgcgaggtgt
     5521 ggagctgcct gcggatggaa atggtggccg ggcagtacct ggacatccag ggccaggcga
     5581 cctcgtcgca ttccgaggcc cgcgcgatgc gcgccgcctg cctcaagagc gcgctgtact
     5641 ccgtgcagcg gccgctcgaa ctcggtgccg ccctcgccgg ggcggacacc gccacgactc
     5701 aggcgttgtg ctcggcgggc cggtgcgtcg gcttggcctt ccagctccgc gacgacctcc
     5761 acgacgtctt cgaggaaccc cggcgcaccg gcaagccgtc gggcggtgac atccgggcgg
     5821 gcaaacccac ctacctcgtc gccgtggcgc gggcccgggc cgaagcggcc ggcgaccgcg
     5881 gcgccctcgc cgtactgcgg cgctcgctcg gttgcgccga tctgtccgac gcggacctcg
     5941 cccgggtgcg ggacgtcctg gtcaccaccg gcgcgcgtga cacggtcgag gccgagatcg
     6001 accggctggt ggcccagggc ctgcgccaca tgggcgccgc ctcactcgcc cccgagggac
     6061 gacgccatct ggacgcactg ctccgttcag cggccggtgc gccctcggtc cgccggcagg
     6121 cccctgacgc gtcccgctcc gagaacggga tgcccgtatc gctgctgctc accgccacgg
     6181 ccgagggggc ctcccgatga acaggacact gcgcgggcgc accgaccacg tcgtggtcgt
     6241 cggtgccgga ctcgccgggc tttccgccgc tctgcacctt ctcggtgccg gtcgccgggt
     6301 caccgtcgtc gaacgcgatc tcctgcccgg aggccgcgcc ggacgtctgg tgcgcggcgg
     6361 ctacctcatc gacaccggtc ccacggtgct gaccatgccc gacctcgccg acgaggcctt
     6421 cgcggcggtc ggcgacagcc tgcgcgaccg tgtcgacctg atcccgctgc accctgccta
     6481 ccgggcccgc ttcgccgacg gcagcaccct cgacgtgcac accggcgccg aggcgatgga
     6541 agcggagatc gaacgcttcg ccggagcacg tgaggcggcc ggctaccgac agctgcgcga
     6601 ctggctcgag cggctgtacc gggcgcagat gcggcgcttc atcgactcca acttcgactc
     6661 ccccctccag ttgctgaccc ccgatctggc gcgtctggcg gcgctcggcg gattcggccg
     6721 actggacgcg cgcatcggcc gccatctgcg ggacgagcgc ctgcgacgcg tcttctcctt
     6781 ccaggccctg tacgcgggcg taccgccggc ccgggccctc gccgcctacg ccgtcatcgc
     6841 ctacatggac accgtggccg gtgtgtactt tccccggggt ggcatgcacg ccctgccgcg
     6901 ggccatggcg gacgccgccg cgcaggcggg tgccgaactg cgcttcggac aggacgtcac
     6961 ccggctggag cgggccggtg accgcatcac cgccgtcgtg acggcgtacg accggattcc
     7021 gtgcgacgcc gtcgtcctca cccccgatct gcccgtcacc taccgcctgc tcggccgtgc
     7081 gccgcgacgc ccgttgcgga tcaggaactc gccgtccgcc gtggtcctgc acgcgggcac
     7141 cgaccggacc tggcccgggc tcgcccacca cacgctctcc ttcggcgcgg cgtggggccg
     7201 cacgttcgac gaactcaccc gcaccggggg cctgatgagc gatccgtccc tgctcatcac
     7261 gcgtcccacc gccaccgacc cgggcctcgc gccgcccggc cgccatctcc actacatcct
     7321 cgccccctgt ccgaacacgg acatcgggcc gagcgccgcc gcatggggcg acctggcgcc
     7381 gcgctaccgg gacagcctcc tgacggaact ggaggcccga ggtctcgacg gcatcggatc
     7441 cgccgtcgaa gaggagtgcc tggtcacacc ggccgactgg gcggcccagg gacacgccgc
     7501 gggcacgccc ttctcggcgg cccacacctt cgcgcagacc ggcccgttcc gtccgcgcaa
     7561 cctggtacgc gggaccgcga acgccgtgct cgccggctgc ggcaccacgc ctggtgtcgg
     7621 cgtgccgacc gtgctgctgt ccggcaagct ggccgcagcc cgtgtcaccg gtggcacgag
     7681 gcgcacaccc cgccgtatgc ccgcctcccg caccgcgagc ggaaggacgc tttcatgacc
     7741 gaccgcgaac tcgatgcggc gggcatcacc gacccgtcgc tgcgcaaggc ctacacgcac
     7801 tgccgccggc tcaacgcgcg gcacggcaag acgtacttcc tcgccaccag actgctgccg
     7861 gtcgaacgcc ggcccgccgt gcacgccctc tacggattcg cacgctgggc cgacgacatc
     7921 gtcgactccc tggacaccac cgtcggcccc gctgtgcgct cggcggcgct cggccgcctc
     7981 caggagagcc tgcggacggg actgcgtgag ggacacagca ccgagccggt ggtcctggcg
     8041 ctcgccgaga cggcccggcg gtacgcgatc gaccaccagc acttcagtga cttcatgacg
     8101 gccatgcgca gcgacctgga ggtcaccggc tacgagacct acgccgacct gcgcggctac
     8161 atgcacggtt cggccgcggt gatcgggctg cagatgctgc cggtgctcgg gaccgtggtc
     8221 ccgcgtgagg aggccgcacc ccacgcggcg gccctgggag tggcgttcca gctgaccaac
     8281 ttcctgcggg acgtgggcga ggacctggac cgcggccgcg tctatctgcc cgccgacctg
     8341 ttgcgcgccc acggcgtcga ccgggacctg ctgcgctgga gcagggacac cggccgccgg
     8401 gaccgccgta tcacccgtgc gctcaaggcc gttgagggcc tgacccgcgg tgtgtaccgc
     8461 gaggcggcgc cggggcttgc gatgctcgac ccggtggcgc gcccgtgcat ccggacggcg
     8521 ttcgtgctgt acggcgggat cctggacgcc atcgccgacg acgggtacgc ggtcgtccac
     8581 cgccgtgccg tggtgccgcg ccggcgccgt gccgcggtgg cgctggacgg tctcgtacgc
     8641 ctgggcgtgg cccgtctggg atcccgcgcc gacgcgcggc tgcccaccct gcccgatccg
     8701 tggcggcttc ccgccctggt gccgaggtcc tcgccccggc cggcacatga ggaggtcgca
     8761 tgacccctga ccgcaccgac cgtcctgccc ggcgccgacg ctacccgctc tcgttgcgca
     8821 ggcatccgat tcgctgggag gaccagcgtc cgacctggcg cgacgcgcgg cccgccgtga
     8881 tcgcggaggc gctgaagcgg gcgcaggcac gaccatcggg caactggtac gtcgtcggcg
     8941 ccacccgtga tgtgcgggag gaccgccctc tggcgcgcac cgtggccggc cttgaggtcg
     9001 tcgtctggcg ggacggcgaa gggcggctgg tcgccgggcc aggtgtctgc ccccatctcg
     9061 gtgcaccgct gcgggacagc cccgtgcgct gcggcacgct catgtgtcgc tggcacggac
     9121 tcgccctgag cggcacgccg ttcgcgggct gggaaccgct gcccgtgcac gacgacggcg
     9181 tgctggtgtg ggtacggctg gacgaggtcg gcggtgagcg accgctcgag gccccggtgg
     9241 taccggccag gcctgccctg tcgggtgccg tgtcggccgt ctacgtcggc gtcggaatct
     9301 gcgaacccga ggatgtcgtc gccaaccgcc tggacccctg gcacggcgca tggttccacc
     9361 cgtactcctt cgtcgacctc accgtcgtca cgcccgggga aaccgtcgca cctgaggaag
     9421 gagaaacgga gggtgtagag acggggggtg acgaggcgga gggcgccgag acggaggacg
     9481 gatttaccgt cgacgtgtct ttcaagatcg ctggacggct ggtcgtcccg gtgcgggccg
     9541 tcttcaccgc tccggagccc cgcacggtcg tgatgcgcat cgcggagggg gaggggcggg
     9601 gctccgtcgt cgagacccat gccaccccgc tcggccctga cgaccggggc aggccgcgca
     9661 cggccgtggt cgaggccgtg ctggcgtcgt ccgaccgccg cgggttcgcc gtggcgcgcg
     9721 cgatggcccc ggtgctgcgg ccactcatgc gcgctgccgc cggacgcctg tggcgcgacg
     9781 acctggcgta cgcggagcgg cgccggcacc tgcgctccac gggccgcttc cccggctgaa
     9841 cgacaggcct gcggtgagcc gacggcccac acgatgccgc acgctcacgg gggcggcggg
     9901 gtggcgtacc tggcacaggc ggcggtgcct caggccttcg gaccggagca cggaacgagt
     9961 tccgccggat atgggcgggg gagccattcc gatgaccgga cccccgcgcc atgtcggctg
    10021 cgggggcccg gtccactcgt ccggctcaac cggtgcggct cacagcccgc tcgaccgaga
    10081 tccgttctgg tcggtcacgt cggtgaccga ccaccgttgg ttggatccgg agttcggcgt
    10141 ccatgtcgtg acggcggctc cgtcggtggt ggcctggccg ccgacctcga ggagcctgcc
    10201 ggtggcggcg ttgacgaggg tccaggtgcc gtcgccggtg gtcgacatga tccactgggt
    10261 ggccttgtca cggtggccgc ggtccgcctc cagcaccggc gcgccgtcac gaacggccag
    10321 gcgctttcct gccgcggggt tggtgaacac gtaccgctgc cggttcccgg tgtcgccgct
    10381 gatctgacgc acctgccact gctgcccggt cccggccgca tccgtacccg cggtcctgat
    10441 gacgagctgg gtgccgtcgt ccgcgacggt gagggccttg ccgctctgga cgccggtcag
    10501 ctggtaggtg tgccccttgc acaggagcgc ggcgtccttc gcgacgccgg acactccctt
    10561 gacgaggaag gacgtcacgg actgggcggg tacggtgacc gcggcccggc tgccggtgac
    10621 ctcgacgggg gcctgtcgtt cgagcttgcc tgcggcgctg gtgaccacgg gagtcacggt
    10681 cgcgtcgcgg gtgatgtggc cgaacttgga caggtcgatc gtgacggtgc gggcgctggt
    10741 ggtcttgttg acgtggacga ccgtcgccgc gttgcccttc gtggagaccg cggcggcgct
    10801 ggaggtgtcg tcggtcctga tcagtcggtc gcccggcttg atgtagtggg tgaagttgcg
    10861 ggcggtgtcg aacttcgtgt tcgtgtggac ggggcaggtg gccagggtgt ccgcggaggt
    10921 gcagctgaac gggagctgga tgctgcccca gttgccgccc ttggcggact caccgccggg
    10981 cttcatgttg tcgtagtcct cgaccggctg ccagaacacc caggccttgg gctcgagttc
    11041 gcgcaggtcg tcgaccatct gctgggcaag cccgaggccg ggccgcatgt ccgtgaagct
    11101 ctggccgtcg ccccagtcgc cctcgacctc gctcatccac agcggcttgt ccgccgcctt
    11161 ggccaggtcg cgcacgctgg tccggccgcc ggtgccgtaa gtgtggacgt tcatctggcc
    11221 gacgagatcg cgcacgtcct gggggtagga gttccagttc tgcgcgaaga ggttcgggtt
    11281 ggtctcgtcc atcgcggaga tcttcgtgcc ggtcttcgcc ttcttcaggg cgggggccag
    11341 cgcgcggatc accttctgct ggagctccgg gccgatgtga gccccttcct gacggccgcc
    11401 gacgggccgg ccgtcggatc ccaggcgcgt accccaatag cccgtgttgg gttcgttgaa
    11461 cgggtcgagg gtgtcgacct tgatgccgtg cgccttctcc agccgcttgg tcgcgcctgc
    11521 cagataggcg gcgaagtcgt cgacggagtc ggccttgagc tggtcttcgc tggagttgaa
    11581 gccgcccgac acatagccgc tggcggtcat gaaccagggc ggggagttgc tgaacgtctc
    11641 ccagtggtcg atgtcgttct tgatccggtc cacccaccac cgctgggtcg cgtcggcgtt
    11701 ggggttccag tcggcggcgt cgtcggcgct ccaccagtcg gtgtcctcac gggtggtgcc
    11761 cgccggggcg ttccaccagc cctcgaccgc tccgccggcc cggaggtagt ccttgacgtc
    11821 gggggcgttg ccgccgccga tgttgtagcg ggcgatgttc agggcgaggc cgtcgtcgtc
    11881 gaagaggagc tcggccagct tctcgcgtat ctccgcggga tagtcgccgg tggcgttggc
    11941 gaaccagacc aggctggtgc cccagccctc gaatctgtcc gctcggtacg aggggtcggg
    12001 ctggaccgtg acggcagcgg tcgccgctgc cgccggggtc tgcgcgttcg tcggtggagc
    12061 ggtgaccagg gctgccccgg tggccagggc ggtgacgccg atggttccca gaagccgtcg
    12121 tgtgcgggta cggcgtgcca acgtatgctc cccactcatc tgtggtcgct tcggtggacc
    12181 gtcgtggcct gagcgccagg acggatggcc ttcgaacagg tgcggtgccc ttcgagggcg
    12241 gtcagcgggt gggctgccgc agaacggcga catcccgggg ggacagcacc acggtgccgt
    12301 cgtcctctgc ccggccgatc aggagttccc cggcgagtcc ggtcagcggc accgtcccgt
    12361 cggtccggtt gaccaggaac agatagcggc tgccggcgcc gcggcgcacg atcagttcga
    12421 cgtctccccc tacgccgtcg ggcagttcac tgccgaccgc ggcagggccg agcagcttcg
    12481 gcagcagtgc ggtgaggccg tccggtccca gccgggtgga cacatacgcg gccgaacccc
    12541 ggcccaccgc acggcgggtg acggcggggt ggccggcgta cgtaccggtg cggtaccggg
    12601 ccaggacctc cacctcgggg tcggtgacgg tgatccggtc ggtccacagg gtgcccgtgg
    12661 cggcgtcgtc cagtgcgacc gtgtcgccgt cgagcagcgg gccgaactcc tcgacgcgga
    12721 tgccgagcag atcgcgcagg gctccggggt atccgcccag ccagatgtgg tcgttctcgt
    12781 ccacgacacc ggagaagtac gtggtgacca ggtgcccgcc gttctcggca tagcgggtga
    12841 gttccttggc cagtgccgcg gggaccacgt gcagcaccgg cgtgatcagg agctggtacc
    12901 ggtcgaggtc ggcctgcggg gtgacgacgt cggcccggat gccgagggcg aggagggcgg
    12961 agtaccagtc gagcgcctcc cggcggtagt cgagcaggga ggtggggtgg gagtcctgct
    13021 cactcgccca ccaggactcc cagtcgaaga cgatcccgac ccgcgccggt tcacgctcgg
    13081 tcccggcgac cggggccagc gtccgaaggg tgccgccgag ggcggtcacc gcgcggaaga
    13141 cctcgctgtc gggtccggcg tgcggcacca tcgccgagtg gtacttctcg gcgcctgccg
    13201 ccgactggcg ccactggaag aagcacacgg cgtcggcgcc gtgcgcgacg tgcagcaggg
    13261 agtcgcgggc caggtcgccg ggccgtttgg ccaggttgac gggctgccag ttgacggcgc
    13321 tggtggagtg ctccatgagg aaccacgggc gtccgccggc tatgccgctg acgaggttgg
    13381 cggagaagga cagctcgtcc cggtcctgag gcccgggcga gacgtagtgg tcgttggaga
    13441 cgaagtcgat ctcctgggcc cagtcggcgt agttcatgcc cttggtgcgg cccatcacca
    13501 tgaagttggt ggtgaccgga acctcgggcg tgatctcgcg cagcaggtcc cgctccgcgc
    13561 gcaggtacgc cttcagcgcg tcggaggaga agcgcttgaa gtccagctgc tgcgtggggt
    13621 tcgggtggga ggcggccagc cggggcggca ggatctggtc ccagtcgctg tagtactggg
    13681 accagaacgc ggttccccag gcgtggttga gggcgtcgag cgtggtgtag cgggcgcgca
    13741 gccagtcgcg gaaggcacgg gcggcgtcgt cggagtagtc gtagatgttg tggcagccca
    13801 gctcgttgga gacgtgccat gccaccagcg cgggatggtc cgcgtaccgc tccgccagct
    13861 tccgcaccag gcgcagggcg tgcgtgcgga agacggggga cgtgggacgc cagtgctgcc
    13921 gcgcccccgg ccacaccgtc tcgccggagg cggtcaccgg gaggatctcc gggtgtgccg
    13981 tggcgagcca gggcggcggg gacgcggtcg ccgtggccag gtcgaccccg atgccgcccg
    14041 cgtgcagcag gtccatgacg tcgtcgagcc aggcgaagtc ccactggccc tgggccggct
    14101 ggatgcgggc ccaggagaag atccccaccg agacgatgtt gacgccggcc tcgcgcatca
    14161 gccggatgtc ttcctcccac acctcgcgcg gccactgctc ggggttgtag tcggcgccgt
    14221 acgcgaggcg tggcgtgcgg tcaccgtccg gcccgttcag ctgggacagg agggtggaga
    14281 tcatcgtggt ccttccggtg tgtc
//
