LOCUS       BGC0000634             11991 bp    DNA     linear   BCT 17-JUN-2020
DEFINITION  Brevundimonas sp. NBRC 101024 carotenoid biosynthesis gene cluster
            (orf1, crtW, crtY, crtI, crtB, orf6, orf7, crtE, idi, crtZ, crtG,
            orf12), complete cds.
ACCESSION   BGC0000634
VERSION     BGC0000634
KEYWORDS    .
SOURCE      Brevundimonas sp. NBRC 101024
  ORGANISM  Brevundimonas sp. NBRC 101024
            Bacteria; Proteobacteria; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Brevundimonas; unclassified Brevundimonas.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-07 20:05:56
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 11991
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..11991
                     /culture_collection="NBRC:101024"
                     /db_xref="taxon:1113698"
                     /mol_type="genomic DNA"
                     /note="Brevundimonas sp. SD212 is distributed from Marine
                     Biotechnology Institute as MBIC03018.; cloning vector:
                     p5Bre2-15"
                     /organism="Brevundimonas sp. NBRC 101024"
                     /strain="SD212"
     protocluster    1..11991
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="True"
                     /core_location="[2566:6155]"
                     /cutoff="20000"
                     /detection_rule="(Terpene_synth or Terpene_synth_C or
                     phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7
                     or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      2567..6155
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(Terpene_synth or Terpene_synth_C or
                     phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7
                     or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
     cand_cluster    1..11991
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Terpene_synth or Terpene_synth_C or
                     phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7
                     or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..11991
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000634"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..11991
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="terpene"
                     /region_number="1"
                     /rules="(Terpene_synth or Terpene_synth_C or phytoene_synt
                     or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps
                     or fung_ggpps2 or trichodiene_synth or TRI5)"
                     /subregion_numbers="1"
                     /tool="antismash"
     CDS             complement(1313..1648)
                     /codon_start=1
                     /note="orf1"
                     /product="hypothetical protein"
                     /protein_id="BAD99405.1"
                     /transl_table=11
                     /translation="MPLALLFALAALSSTPAPAADPAKAQPKTSGGIKAAAQSSAPVLD
                     DVRVRLRCIGRADGRVSDCVVLSESRPGYGFGEAAVALMNGTATPPSRDGGRPIDRPFE
                     HTIQFTP"
     gene            complement(1753..2487)
                     /gene="crtW"
     CDS             complement(1753..2487)
                     /codon_start=1
                     /gene="crtW"
                     /note="4,4'-beta-oxygenase"
                     /product="beta-carotene ketolase"
                     /protein_id="BAD99406.1"
                     /transl_table=11
                     /translation="MTAAVAEPRIVPRQTWIGLTLAGMIVAGWGSLHVYGVYFHRWGTS
                     SLVIVPAIVAVQTWLSVGLFIVAHDAMHGSLAPGRPRLNAAVGRLTLGLYAGFRFDRLK
                     TAHHAHHAAPGTADDPDFYAPAPRAFLPWFLNFFRTYFGWREMAVLTALVLIALFGLGA
                     RPANLLTFWAAPALLSALQLFTFGTWLPHRHTDQPFADAHHARSSGYGPVLSLLTCFHF
                     GRHHEHHLTPWRPWWRLWRGES"
     gene            2567..3745
                     /gene="crtY"
     CDS             2567..3745
                     /codon_start=1
                     /gene="crtY"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: Lycopene_cycl"
                     /gene_kind="biosynthetic"
                     /product="lycopene beta-cyclase"
                     /protein_id="BAD99407.1"
                     /sec_met_domain="Lycopene_cycl (E-value: 7.8e-119,
                     bitscore: 386.7, seeds: 20, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQAAAPDSSAPDLLLLGGGLANGLLALRLSQVRPELDVRIVEAAD
                     RLGGIHTWSFFEADLTPAQRAWIAPLIACRWPGYSVRFPAFERRLVTGYCSVTAERFAE
                     AVTQALAGRIVTGAAVVSAGPTEAVLADGHRLTARAVIDGRGPTAAPDLALGFQKFVGL
                     EVRLTAPHGLKEPIVMDACVDQSGGYRFLYVLPFDDRTLLIEDTRYTDGDDLDHDLFRT
                     GVRDYAAQRGWVIETVLREEEGVLPVALDGDIAAHLKRLGPTALSGLRAGLFHPTTGYS
                     LPDAVRLADHLAERIEAAPDGPALAQVIRRHARDVWAQRGFYRLLNRMLFRAARPDQRY
                     RVLERFYRLPQPLIERFYAGETTLADKARILSGKPPVPIGAALTCLVERGRA"
     gene            3745..5226
                     /gene="crtI"
     CDS             3745..5226
                     /codon_start=1
                     /gene="crtI"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222:dehydrogenase (Score: 430.8; E-value: 1.4e-130)"
                     /gene_kind="biosynthetic-additional"
                     /product="phytoene desaturase"
                     /protein_id="BAD99408.1"
                     /transl_table=11
                     /translation="MRAAVIGSGFGGLSLAIRLQTAGIQTTVFEARDLPGGRAYVYKDK
                     GYTFDAGPTVITDPSALEELFEGAGRKLSDYVELLPVAPFYRLCWEDGDVFDYVNGQDE
                     LDRQIVARNPADKEGYRRFLAYSQDLLKEGYLKLGAVPFLDFASMVKAAPELMRLQAWR
                     SVYDKVAGYIQDEHLRQAFSFHSLLVGGNPFATSSIYALIHALERRWGVWFPRGGTGAL
                     IQAMVRLFQDLGGEIRLNSPVERITLANGRADGVVVGGQALAFDMVASNADVVHTYQRL
                     LGQEPRGRKEGARLASKRHSMSLFVIYFGLKRVHPEVRHHTVLFGPRYRELIGEIFKGP
                     DLPQDFSLYLHAPTRTDPSLAPEGCDAFYVLAPVPHLASADIDWAVEGPRYRDRVLAYL
                     EQHYIPGLTAHLDTCRIFTPVDFRDQLNAHQGSAFSLEPILTQSAYFRVHNRDDQIPNL
                     YFVGAGTHPGAGVPGVVGSAKATAGLMIEDAGRTA"
     gene            5223..6155
                     /gene="crtB"
     CDS             5223..6155
                     /codon_start=1
                     /gene="crtB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: phytoene_synt"
                     /gene_kind="biosynthetic"
                     /product="phytoene synthase"
                     /protein_id="BAD99409.1"
                     /sec_met_domain="phytoene_synt (E-value: 2.6e-54, bitscore:
                     173.9, seeds: 8, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDAVLDHSRQSMEQGSKSFAAAARLFPAAIRDDAWMFYAWCRHC
                     DDEIDGQVLGHGAVGIDPVLAGRKLVELRERTAAALAGEPQTDPVFTAFQRVAARHAIP
                     AEEAMDLLQGFEMDVEGRRYDTLEDTLDYAYHVAGVVGVMMARIMGVQDAPTLRRAQDL
                     GLAFQLTNIARDVVEDAKGGRVYLPGQWLDEAGVPRDQVDQPRHRQAVAHTAQRLVAAA
                     EPYYASARWGLRDLNPRSAWAVATARGVYRAIGRHVSRSGATAWDGRTSVDKAGKLALV
                     GRGALITLWCKTLDAWREPPPRPALWTHI"
     CDS             complement(6162..7226)
                     /codon_start=1
                     /note="orf6"
                     /product="hypothetical protein"
                     /protein_id="BAD99410.1"
                     /transl_table=11
                     /translation="MAVAGAWARGFWRDRSGMALTLVLPPLVYLLFAAVFGAGARGDID
                     ASVALHDAARTPAAAAVGEALSRDLGPRLRRLDDAQAVERAVIDGRADAGVLITRDDAG
                     APQLTVVSAAGRDVAAAALQARLVPAAAALAGRPAPPSRDVPVRQVGPQGDVQAAYYAG
                     AVSVMFVFFAAMHGAMGGLDERRSGLQARLALARGGTAPILAGRAIWLTTVGLMQSAAV
                     FLAAWPRLPDLAFWQAAAALLTAALVALCAAGVALALTAACRSREQAQPLTTFVALLLA
                     ALGGSMAPRFLMPEAFRALGWITPHAWAVETYQALIWRGDFNAGVLAGWAALGGVGLAG
                     IALAVMMEHRRAGR"
     CDS             complement(7226..8173)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 172.7; E-value:
                     1.7e-52)"
                     /gene_kind="transport"
                     /note="orf7"
                     /product="hypothetical protein"
                     /protein_id="BAD99411.1"
                     /transl_table=11
                     /translation="MSFISSGRPNLDASVAPALVLKDVEAGYGEGPVLRDFNLTVAAGE
                     VYALLGPNGAGKSTAARVACGLIPVRRGRATAGEGAPRRGRIGLAPQDCALFPALTPRE
                     NVAAVAALCGLPRSNRPAAVERALALTGCAPRADEPVHSLSGGWRRRANLSAALVGRPR
                     LLIADEPTEGVDAAARVVLSSALRETVQAGAGCLLISHDEAFVSETADRIGVLAQGRLL
                     AEGPPKALLRQAFGLARLLVVKLPRPASRLAASRFAQSGLAPSKDGLEWRLLCEDALGA
                     AQRVSPEADAEGGEVAVRRPGLDDLVAHLSDASS"
     gene            complement(8133..9029)
                     /gene="crtE"
     CDS             complement(8133..9029)
                     /codon_start=1
                     /gene="crtE"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182:Polyprenyl synthetase (Score: 177.6; E-value:
                     4.9e-54)"
                     /gene_kind="biosynthetic-additional"
                     /note="geranylgeranyl diphosphate synthase"
                     /product="GGPP synthase"
                     /protein_id="BAD99412.1"
                     /transl_table=11
                     /translation="MAIVGLRPQPVSDPEPQSPENLRGLVQDRLAETAPSSDGLLALAA
                     REALLGPGKRVRPVVAMLAAAHVGGRAEDALDFGCAVEMAHAASLVLDDLPCMDDAALR
                     RGQPTLHRRHGEDAAVLAAVALLNQSTRLILQSRAPSEARLGALDDLTQAIGFDGLAEG
                     QMRDLRDDPVQRDVVALRRINDLKTGALFVAAARGGGRMGGGDADDLARLAAFGEAVGF
                     AFQLCDDLMDACSTSEALGKDVGQDQGVTTFVDLWGEGRVRAGVRQSLARAAEAVGHDS
                     PLTTYVLHLFRQAELGR"
     misc_feature    complement(8907..8909)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(9012..9014)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            9169..10221
                     /gene="idi"
     CDS             9169..10221
                     /codon_start=1
                     /gene="idi"
                     /note="isopentenyl diphosphate isomerase"
                     /product="IPP isomerase"
                     /protein_id="BAD99413.1"
                     /transl_table=11
                     /translation="MPTPDDALIRRKDEHIDHVRAGRGLSGASSGLEAVRFVHDALPDL
                     ALDQIDLSARFLGRRLNLPFLISSMTGGPSRAEAINARLAEAAQALGVALAVGSQRVAL
                     ETAGGSGGSGLGPDLRRRAPDALILANLGAVQFALGYGVDEARRAMEMIGADALILHLN
                     PLQEGVQPEGDRDWRGVAQGIERIAAAFPGQVVVKETGAGLSAAVARRLADMGVAALDV
                     AGAGGTNWGLIEGARATGGRAEALAAPFADWGVPTARSLRDCAQAAPDLGLIGSGGIKD
                     GLDAARAVRLGADLVGQAAGVLEAALTSTQAVVDHFELMAAQLRLACFCTGSADLAALA
                     QAPLLEEPRF"
     gene            complement(10188..10673)
                     /gene="crtZ"
     CDS             complement(10188..10673)
                     /codon_start=1
                     /gene="crtZ"
                     /note="3,3'-beta-hydroxylase"
                     /product="beta-carotene C3-hydroxylase"
                     /protein_id="BAD99414.1"
                     /transl_table=11
                     /translation="MAWLTWIALFLTAFLGMEAFAWIMHRYVMHGFLWSWHRSHHEPHD
                     HPLEKNDLFAVVFAAPAIVMVAVGLHLWPWALPVGLGITAYGMVYFFFHDGLVHRRFPT
                     GFSGRSGFWTRRIQAHRLHHAVRTREGCVSFGFLWVRSARALKAELAQKRGSSSSGA"
     gene            10748..11521
                     /gene="crtG"
     CDS             10748..11521
                     /codon_start=1
                     /gene="crtG"
                     /note="2,2'-beta-hydroxylase"
                     /product="carotenoid C2-hydroxylase"
                     /protein_id="BAD99415.1"
                     /transl_table=11
                     /translation="MLRDLLITTLALSLIIGLRYLLVGAAAHGLLWAGAGRGRALNLRP
                     PAMKRIRAEIVASLIACPIYALPAALVLELWKRGGTAIYSDPDAWPLWWLPVSLIVYLL
                     AHDAFYYWVHRALHHPRVFGWAHAEHHRSRDPSAFASFAFDPAEAAATAWFLPALALIV
                     PIHWGVALTLLTLMSLTAALNHAGREVWPAAWLERAPLRWLITATHHDAHHKRFNGNYG
                     LYFQFWDRWAGTEVSAAPSPPSPVIPPERPSAPLR"
     CDS             complement(11416..11784)
                     /codon_start=1
                     /note="orf12"
                     /product="hypothetical protein"
                     /protein_id="BAD99416.1"
                     /transl_table=11
                     /translation="MLKRLGCQVLTAATVNDGLRIVEDWSPDAVLVDILMPDRDGLNFI
                     MDTRHLRDSLRVIAMSGGGRLASSSILQMATGLGAHARPDQADHRRGAEGRSGGMTGDG
                     GEGAAETSVPAQRSQNWK"
ORIGIN
        1 gaattccccg tgaagatgcg gggttcccgc ggtcagacgg aaagacccta tgaaccttta
       61 ctatagcttc gccttggcgt tagcgaccgt atgtgtagga taggtgggag actatgaaac
      121 cggggcgcca gctctggtgg agtcgtcctt gaaataccac ccttactgtc gttgacgtct
      181 aaccgaggac cgttatccgg tcccgggaca tggcgtggtg ggtagtttga ctggggcggt
      241 cgcctcccaa agtgtaacgg aggcgcgcga tggtgagctc agagcggtcg gaaatcgctc
      301 gtcgagtgca atggcataag ctcgcctgac tgcgagactg acaagtcgag cagagacgaa
      361 agtcggccat agtgatccgg tggtcccgag tggaagggcc atcgctcaac ggataaaagg
      421 tactctaggg ataacaggct gattttgccc aagagtccat atcgacggca aagtttggca
      481 cctcgatgtc ggctcatcac atcctggggc tggagcaggt cccaagggta tggctgttcg
      541 ccatttaaag tggtacgtga gctgggttca gaacgtcgtg agacagtttg gtccctatct
      601 gccgtgggtg ttcgaagctt gagaggatct gtccctagta cgagaggacc gggatggaca
      661 tacctctggt gtacctgtca tggcgccagc tgtgcagcag ggtagctaag tatggaatag
      721 ataaccgctg aaagcatcta agcgggaaac taacctcaaa acaaggcttc gctgaggatc
      781 gtggaagact accacgttga taggccaggt gtggaagcgc ggcgacgcgt gaagcttact
      841 ggtactaata atccgatcgg tttgatcgtt tctcagcaaa actcattcga tgatcatcaa
      901 tgacccgatg atcgtcacga caatgtcttc tcatcatccg ctgtccgcct cgttgacctg
      961 gtggctatgt cggaggttcc ccacccgatc ccattccgaa ctcggtcgtt aagccctcca
     1021 gagccaatgg tacttcgtct caaggcgcgg gagagtaggt cgccgccggg tctaccaggc
     1081 ggacagccga tgatgaaaaa cctatcgaac ccttcttcct ccacaaacca cccctgccgc
     1141 gggatggagc agcccggtag ctcgtcaggc tcataacctg aaggtcgcag gttcaaatcc
     1201 tgctcccgca cccaaacaat caagccgctg gatcaacgat ccagcggctt tttgctgcct
     1261 gaacccccaa gcccgcgccc ccatcccgga cccgaacgcc aagcgtcggc tctcaaggag
     1321 tgaactggat cgtatgttcg aacgggcggt cgatcgggcg gccgccgtcg cgcgaggggg
     1381 gcgtcgcggt tccgttcatc agggccacag ccgcctcgcc gaacccgtag cccggacggc
     1441 tctccgacag cacgacgcaa tccgagaccc ggccgtcggc acgcccaatg catcgcagtc
     1501 gaaccctgac gtcgtccaag acgggcgctg aggattgggc ggccgccttg atcccgccgg
     1561 acgtcttggg ctgcgccttg gccggatcgg cggcgggcgc cggcgtcgaa ctgagggcgg
     1621 caagagcgaa cagcagggcg aggggcatgg gccatttcct tctcacctat aaggcccgga
     1681 acgccgccgt gttcccttcg ccgacacaaa ggtcgccgaa caggcgttcg gcggcccgca
     1741 agcagccttg gatcaagact cgccgcgcca caaacgccac cagggccgcc agggggtgag
     1801 gtggtgttcg tggtggcggc cgaagtggaa gcaggtgagc agggaaagaa cggggccgta
     1861 gccgctgctg cgggcgtggt gggcgtcggc gaacggctgg tcggtgtggc ggtgcggcag
     1921 ccaggtgccg aaggtgaaga gctgaagcgc tgaaagcagg gccggcgcgg cccagaaggt
     1981 caggagattg gccggccgcg cccccaggcc gaagagggcg atcaggacca gggcggtcag
     2041 gaccgccatc tcgcgccagc cgaaataggt gcgaaagaag ttcaggaacc agggaaggaa
     2101 ggcgcggggc gccggggcgt aaaagtccgg gtcgtcggcc gtgccgggcg cggcgtggtg
     2161 ggcgtggtgc gccgtcttca gccgatcgaa gcggaagccc gcatagagcc ccagggtcag
     2221 ccggccgact gcggcgttca gccgcggccg tcccggcgcc agggagccgt gcatggcgtc
     2281 atgggcgacg atgaaaaggc cgaccgacaa ccaggtctgg accgctacga tcgccgggac
     2341 gatcaccaga ctggaggtgc cccagcggtg aaaatagacg ccgtagacgt gcaggctccc
     2401 ccatcccgcc acgatcattc ccgccagggt cagaccgatc caggtctggc gcgggacgat
     2461 gcggggctcg gcgacggcgg cggtcatgga ccttgtttaa cccaggccgg cgtcggacgc
     2521 atccgggcgg cttgcccgcc acgcccgccc gtgccacctg tcgcggatgc aggccgccgc
     2581 ccccgacagt tccgcgccgg accttttgct gctggggggc ggcctggcca acggccttct
     2641 ggccctgcgc ctgtcccagg tccggcccga actggacgtg cggatcgtgg aggcggccga
     2701 ccggctgggc gggatccata cctggtcctt cttcgaggcc gatctgacgc cggcgcagcg
     2761 ggcgtggatc gcgcccctga tcgcctgtcg ctggcccggc tattccgtgc ggtttccggc
     2821 gttcgaacgg cggttggtca ccggctattg cagcgtgacg gccgaacggt tcgccgaggc
     2881 ggtgacccag gccctggcgg ggcgcatcgt caccggcgcc gccgtcgtct cggccgggcc
     2941 gaccgaggcg gtcctggcgg acgggcaccg gctgacggcc cgggcggtca tcgacggccg
     3001 ggggccgacc gctgcgccgg acctggccct ggggtttcag aaattcgtcg gcctggaggt
     3061 gcggctgacc gcgcctcacg ggctgaagga accgatcgtc atggacgcct gcgtcgatca
     3121 gtcagggggc tatcgcttcc tctatgtcct gcccttcgac gaccggaccc tgctgatcga
     3181 ggacacccgc tacaccgacg gcgacgacct ggatcacgac ctgttccgaa cgggcgtcag
     3241 ggactacgcc gcgcagcggg gctgggtcat agagacggtt ctgcgcgagg aggagggggt
     3301 gctgccggtc gccctggacg gcgacatcgc cgcccatctg aagcggctgg ggccgacggc
     3361 gctgagcggc ctgcgcgccg gtctgtttca tccgactacc ggctattccc tgccggacgc
     3421 ggtgcggctg gcggatcatc tggcggagcg tatcgaagcg gcgccggacg gcccggccct
     3481 ggcccaggtc atccgtcgcc atgcgcgcga cgtatgggcg caaagaggct tttatcggct
     3541 gctgaaccgc atgctgtttc gggccgcgcg gccggatcag aggtacaggg tgctggagcg
     3601 gttctatcgc ctgcctcagc cgctgatcga acgcttctat gcgggggaga cgaccttggc
     3661 cgacaaggcg cggatcctca gcggcaaacc cccggtgccg atcggcgccg ccctgacctg
     3721 tctggtcgaa agaggacgtg cgtgatgcga gcagcagtga tcggatcggg gttcgggggg
     3781 ctgtcgctgg ccattcgcct tcagacggcg gggatccaga ccacggtctt cgaggcgcgc
     3841 gacctgccgg gcggccgggc ctatgtctat aaggacaagg gctatacctt cgacgccggg
     3901 ccgaccgtca tcaccgatcc ttcggcgctg gaggagctgt tcgagggcgc ggggcgcaag
     3961 ctgtcggact atgtcgaact gctgccggtc gcccccttct atcggctgtg ctgggaagac
     4021 ggcgacgtct tcgactacgt caacggccag gacgagctgg accgccagat cgtcgcccgc
     4081 aacccggccg acaaggaggg ctatcgccgg ttcctggcct attcccagga cctgctgaag
     4141 gaaggctatc tgaagctggg cgccgtgccc tttctggact tcgccagcat ggtcaaggcg
     4201 gcgccggagt tgatgcggct ccaggcctgg cggtcggtct atgacaaggt cgccggctat
     4261 atccaggacg agcatctgcg tcaggccttc agctttcact ccctgctggt gggcggcaat
     4321 ccgttcgcca cctcatcgat ctacgccctg atccacgcgc tggagcggcg ctggggcgtc
     4381 tggttcccgc gcggcggcac cggcgccctg atccaggcca tggtgcggct gtttcaggac
     4441 ctgggcggcg aaatccggct gaacagtccg gtcgagcgga tcaccctggc gaacgggcgc
     4501 gccgacgggg tggtggtcgg cggccaggcc ctggccttcg acatggtcgc ctccaatgcg
     4561 gacgtggtcc acacctatca gcgcctgctg ggccaggagc cgcgcggccg caaggagggg
     4621 gcgcgtctgg cctccaagcg gcattccatg tccttgttcg tcatctattt cggcctgaag
     4681 cgggtccacc cggaggtgcg ccaccacacg gtgcttttcg gcccgcgcta ccgcgagctg
     4741 atcggcgaaa tcttcaaggg gccggacctg ccccaggact tttccctcta tctgcacgcc
     4801 ccgacccgca ccgatccgtc cctggcgccc gagggatgcg acgccttcta tgtgctggcg
     4861 ccggtgccgc acctggcctc ggccgacatc gactgggcgg tcgaggggcc gcgctatcgc
     4921 gaccgggtcc tggcctatct ggagcagcac tacattcccg gcctgacggc ccatctggac
     4981 acctgccgca tcttcacgcc cgtggatttc cgcgaccagc tgaacgccca ccagggctcg
     5041 gccttctcgc tggagccgat cctgacccag agcgcctatt tccgcgtcca taatcgcgac
     5101 gaccagatcc ccaacctcta tttcgtcggc gccggcaccc atccgggcgc gggcgtgccg
     5161 ggggtggtgg gctcggccaa ggccaccgcc ggcttgatga tcgaagatgc ggggcggacc
     5221 gcatgagcga cgccgtcctg gaccacagcc gccagtcgat ggagcagggc tccaagagct
     5281 ttgcggccgc cgcccggctg tttccggcgg ccattcggga cgacgcctgg atgttctacg
     5341 cctggtgccg ccattgcgac gacgagatcg acggccaggt cctgggccat ggggcggtcg
     5401 gcatcgaccc ggtcctggcg gggcgcaaac tggtcgaact gcgcgaacgc acggccgccg
     5461 ccctggccgg agagccgcag acggacccgg tcttcaccgc ctttcagcgc gtcgccgccc
     5521 gccacgccat tccggcagag gaggcgatgg acctgttgca ggggttcgag atggacgtgg
     5581 agggccgccg ctacgacacc ctggaggaca cgctggacta cgcctatcac gtcgccggcg
     5641 tggtcggggt gatgatggcc cggatcatgg gggttcagga cgcgccgacc ctgcgccgcg
     5701 cccaggacct gggcctggcc tttcagctga ccaacatcgc ccgagacgtg gtggaggacg
     5761 ccaagggcgg gcgggtttat ctgcccggcc agtggctgga cgaggcgggc gtgccgcgcg
     5821 accaggtcga tcagccccgg catcgtcagg ccgtcgccca tacggcccag cggctggtgg
     5881 cggcggcgga gccctattac gcctcggcgc gctggggctt gcgcgatctc aatccgcgct
     5941 cggcctgggc cgtcgccacg gcgcggggcg tctatcgcgc catcggccgc cacgtctcgc
     6001 gctcgggcgc cacggcctgg gacggccgga cctcggtcga caaggcgggc aagctggccc
     6061 tggtggggcg cggggccctg atcaccctgt ggtgcaagac cctggacgcc tggcgtgaac
     6121 cgccgccgcg cccggccctg tggacccaca tctgacggcg ctcagcgccc ggcgcgtctg
     6181 tgctccatca tcacggccag ggcgatcccg gccagaccca cgccgcccag ggcggcccag
     6241 ccggccagga ccccggcgtt gaagtcgccg cgccagatca gggcctgata ggtctccacg
     6301 gcccaggcat ggggcgtgat ccagcccagg gcgcggaagg cttcgggcat caggaagcgc
     6361 ggcgccatcg acccgcccag ggccgccagc agcagggcga cgaaggtggt caggggctgg
     6421 gcctgttcgc gcgaccgaca ggccgccgtc agggccaggg ccacccccgc cgcgcacagg
     6481 gcgaccaggg cggcggtcag gagcgccgcc gccgcctgcc aaaacgcaag atccggcagc
     6541 cgaggccagg ccgccaggaa gacggccgcc gactgcatca ggccgaccgt cgtcagccag
     6601 atcgcccgtc ccgccagtat gggcgccgtc ccgccccgcg ccagggccag ccgcgcctgc
     6661 aggcccgagc gccgttcgtc caacccgccc atggcgccgt gcatggcggc gaagaagacg
     6721 aacatcacgc tgaccgcccc ggcgtaatag gcggcctgga cgtcgccctg cggccccacc
     6781 tggcggacgg ggacgtcgcg cgacgggggc gcgggccgac cggccagggc cgccgccgca
     6841 gggaccagcc gcgcctgaag cgccgccgcc gccacgtctc gacccgccgc cgacaccacc
     6901 gtcagctggg gcgcgcctgc atcgtcgcgg gtgatcagaa cgccggcgtc ggcgcggccg
     6961 tcgatcacgg cccgctccac cgcctgggcg tcgtccagac ggcgaaggcg cggccccaga
     7021 tcccgcgaca gcgcctcgcc gacggcggcc gcggccgggg tgcgcgccgc atcgtgcagg
     7081 gccacgctgg cgtcgatgtc gccacgcgcc ccggcgccga agacggcggc gaacagcaga
     7141 tagaccaggg gcggcaaaac cagggtcagg gccatgcccg aacggtcccg ccagaagccg
     7201 cgcgcccagg cgcccgccac cgccatcatg acgaggcgtc cgacagatgg gcgaccaggt
     7261 cgtccaggcc ggggcgacgc acggcgacct cgcccccctc ggcgtccgcc tcgggcgaaa
     7321 ccctctgggc cgcgcccagg gcgtcctcgc acagcagccg ccattccagc ccgtccttgg
     7381 agggcgccag acccgactgg gcgaaccggc tcgcggccag gcgcgaggcg ggccgcggca
     7441 gtttgacgac cagcagccgc gccaggccga aggcctgacg cagcagggcc ttgggcggtc
     7501 cttccgccag cagccggccc tgggccagga cgccgatccg atcggccgtc tcggagacga
     7561 aggcctcgtc gtggctgatc agcagacagc cggcgcccgc ctggaccgtc tcgcgcaggg
     7621 cggacgacag gacgacgcgg gcggcggcgt ccaccccttc ggtcggttcg tcggcgatca
     7681 gcaggcgcgg gcgcccgacc agggcggcgc tgaggttggc gcgccgacgc catccgcccg
     7741 acagtgaatg aaccggctcg tccgccctgg gggcgcatcc ggtcagggcc agggcccgct
     7801 ccaccgccgc agggcggttg gaacggggaa ggccgcacag ggccgcgacc gccgcgacgt
     7861 tctcgcgcgg cgtcagggcg ggaaagaggg cgcagtcctg gggcgcaagg ccgatgcgtc
     7921 cgcgacgggg cgcgccttcg ccggccgtcg cccgcccgcg ccggacgggg atcaggccgc
     7981 aggccacccg agccgccgtg gacttgcccg ccccgttcgg acccagcagg gcgtaaacct
     8041 ctccggccgc gacggtcagg ttgaaatcgc gcagcaccgg cccttcgccg tagcccgcct
     8101 cgacgtcctt cagaaccagc gccggcgcga cgctagcgtc caagttcggc ctgccggaag
     8161 agatgaagga cataggtcgt cagggggctg tcgtgcccga ccgcctcggc cgcccgggcc
     8221 agtgactggc gcaccccggc gcggacccgg ccttcgcccc acaggtccac gaaggtggtc
     8281 acgccctgat cctgacccac gtccttgccc aaggcctcgc tcgtcgaaca ggcgtccatc
     8341 aggtcgtcgc aaagctggaa ggcgaagccg accgcttcgc cgaaggcggc gagacgcgcc
     8401 aggtcgtccg catcgccgcc gcccatccgg ccgccgcccc gcgcggccgc gacgaacagg
     8461 gcgccggtct tcagatcgtt gatccgacgc agggcgacca cgtcgcgctg aacgggatcg
     8521 tcgcgcagat cgcgcatctg gccctcggcc aggccgtcga agccgatcgc ctgcgtcaaa
     8581 tcgtccaggg cgcccagccg cgcctccgac ggcgcccggc tttgcagaat cagccgggtc
     8641 gattggttca aaagggccac ggccgccagc acggccgcat cctcgccgtg gcggcggtgc
     8701 agggtcggct gaccgcgccg caaggccgca tcatccatac agggcaggtc gtccagaacc
     8761 agggaggcgg catgggccat ttcgaccgcg caaccgaaat ccagggcgtc ctcggcccgc
     8821 ccgccgacgt gcgcggcggc caacatggcc acgaccggcc tgacccgctt gcccggtccc
     8881 agcagggcct cgcgcgcggc gagggctaaa agaccgtccg atgagggcgc cgtctcggcc
     8941 agccggtcct gcaccaggcc acgcagattc tcgggcgact gaggttcggg gtccgacacg
     9001 ggctggggcc ttaagccgac gatcgccata cgggtctcct gatgtccttc gacagtcgcg
     9061 ttgcgaccct gggctgacgc attatgatca catacggcat cacgactgcg tcggatgcaa
     9121 cccgccagag catgatcgtt tcggagggaa ccgctaggag cgtttgcgat gcccaccccc
     9181 gacgacgccc tgatccgacg caaggacgaa cacatcgacc acgtgcgggc cggacggggt
     9241 ctcagcggcg cgtcctcggg actggaggcg gtgcgtttcg tccatgacgc cttgccggac
     9301 ctggccctcg accagatcga cctgtccgcc cgtttcctgg ggcggcggct gaacctgccc
     9361 ttcctgatca gttcgatgac cggcggcccg tcgcgggccg aggcgatcaa tgcgcggctg
     9421 gccgaggcgg cccaggccct gggcgtggcc ctggcggtcg gctcccagcg cgtcgccctg
     9481 gagacggccg gcgggtccgg cggctcgggc ttggggccgg atctgcgccg ccgggcgccc
     9541 gacgccctga tcctggccaa tctgggggcg gtgcagttcg ccctgggcta tggggtggac
     9601 gaggcgcgcc gggccatgga gatgatcggc gccgacgccc tgatcctgca tctgaatccg
     9661 cttcaggaag gcgtccagcc cgagggcgac cgcgactggc gcggcgtggc ccaggggatc
     9721 gagcggatcg ccgccgcctt tccgggccag gtggtggtca aggagaccgg cgccggcctg
     9781 tcggccgccg tcgcccggcg cctggccgac atgggcgtcg cggccctgga cgtggcgggg
     9841 gcgggcggca ccaactgggg cctgatcgaa ggggcgcggg ccaccggcgg gcgggccgag
     9901 gccttggccg cccccttcgc cgactggggg gtgccgacgg cccgcagcct ccgcgactgc
     9961 gcccaggccg ccccggacct ggggctgatc ggctcgggcg ggatcaagga cggtctggat
    10021 gcggcccgcg ccgtccgcct gggggccgat ctggtcggcc aggccgccgg ggtgctggag
    10081 gcggccctga cctcgaccca ggcggtggtc gatcatttcg agctgatggc ggcccagctg
    10141 cggctcgcct gtttctgcac gggctccgcc gatctggcgg ccctcgctca ggcgccgctg
    10201 ctggaagagc cccgcttctg agccagttcg gccttcagcg cccgcgccga ccgcacccac
    10261 agaaagccga aggagacgca gccttcgcgc gtgcgcacgg cgtgatgcag acggtgcgcc
    10321 tggatgcgcc gcgtccagaa gccggaccgc ccggaaaagc ccgtcgggaa ccgccggtgc
    10381 accaggccgt cgtggaagaa gaaatagacc atcccatagg ccgtgatccc caggccgacc
    10441 ggcagggccc agggccacag gtgcagaccc acggccacca tgacgatggc cggggcggcg
    10501 aagaccacgg cgaacaggtc gttcttctcc agggggtgat cgtgcggctc atgatggctg
    10561 cggtgccagg accacaggaa accgtgcatc acatagcggt gcatgatcca ggcgaacgcc
    10621 tccatgccca aaaaggcggt caggaacagc gcgatccacg tcagccaggc catggcccca
    10681 gcataggcgg tttgatccgc cttcgccacg acttgaccgc cgcagggatt tgaccgtctg
    10741 tgcccccatg ttgagggatc tgctcatcac caccctggcg ctgagcctga tcatcggcct
    10801 gcgctatctg ctggtcggcg cggcggccca tgggctgctg tgggccgggg cgggccgggg
    10861 acgggcgctg aacctgcggc cgccggcgat gaagcgcatc cgcgccgaga tcgtcgcctc
    10921 cctgatcgcc tgccccatct acgccctgcc ggcggccctg gtgctggagc tgtggaagcg
    10981 gggcgggacg gcgatctaca gcgatcccga cgcctggccc ctgtggtggc tgccggtcag
    11041 tctgatcgtc tatctgctgg cgcacgacgc cttctactac tgggtgcaca gggccctgca
    11101 tcacccgcgc gtcttcggct gggcccatgc cgaacaccac cggtcgcgcg accccagcgc
    11161 cttcgcctcc ttcgccttcg acccggccga ggctgcggcc accgcctggt tcctgcccgc
    11221 cctggccctg atcgtgccga tccactgggg cgtggccctg accctgctga cgctgatgtc
    11281 gctgacggcc gccctgaacc atgcggggcg cgaggtctgg cccgccgcct ggctggagcg
    11341 ggcgccgctt cgctggctga tcaccgccac ccaccacgac gcccaccaca agcggttcaa
    11401 cggaaactac ggcctctatt tccagttctg ggaccgctgg gccgggactg aggtttcggc
    11461 cgccccctcg ccaccatccc cggtcatccc tccagagcgg ccctcagcgc ctcttcggtg
    11521 atcggcttgg tcagggcggg cgtgggcgcc caggccggtc gccatctgca gtatggacga
    11581 cgaggccaga cgtcccccgc cgctcatggc gatgacccgc agggagtccc tcaaatgccg
    11641 ggtgtccatg atgaagttca gcccgtcgcg gtccggcatc agaatgtcca ccagcacggc
    11701 gtcgggcgac cagtcctcga cgatccgcaa cccgtcgttg accgttgctg cggtcaggac
    11761 ttggcaaccc agccgtttca gcatctcctc cagatgaagc agaaccagcg aatcgtcctc
    11821 gatcacgcag actttcacgc ccaacctcca gatgcgatca gggggaacta acggatgaat
    11881 cccatgttgc gtcaactcgg aagacggcgt ttccgactgg ccatcgcctt ggcgggcgcg
    11941 gtcgtgaccc tgcttctggc ggccactggg gtgacgctgc aacgagaatt c
//
