LOCUS       BGC0000636             14398 bp    DNA     linear   BCT 26-JUL-2016
DEFINITION  Brevibacterium linens carotenoid biosynthetic gene cluster, complete
            sequence.
ACCESSION   BGC0000636
VERSION     BGC0000636
KEYWORDS    .
SOURCE      Brevibacterium linens
  ORGANISM  Brevibacterium linens
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Brevibacteriaceae; Brevibacterium.
COMMENT     ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2024-10-17 13:57:48
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 14398
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..14398
                     /db_xref="ATCC:9175"
                     /db_xref="taxon:1703"
                     /mol_type="genomic DNA"
                     /organism="Brevibacterium linens"
                     /strain="DSM 20426; ATCC 9175"
     subregion       1..14398
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="terpene"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..14398
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     CDS             <1..1373
                     /codon_start=3
                     /inference="non-experimental evidence, no additional
                     details recorded"
                     /note="ORF1; similar to Escherichia coli protein of unknown
                     function, gi|1787049"
                     /product="hypothetical protein"
                     /protein_id="AAF65580.1"
                     /transl_table=11
                     /translation="MRPSTGSSFQPWVDQVEVHRVDLDDGDGLVEAATGVDVIHHLVHS
                     MGSGHDFEKKEAATARRVAEAAEAAGVRRIVYLGGLHPDEADLSMHMRSRAHVGRILLD
                     SPVPTIAFQAGIIIGSGSASFEMVRHLAMTLTLMPAPNWVNNRVEPLAVRDVLYYLLKA
                     VDLDEDVNRAFDIGSHDVHKYADIMKRFGAIAGLKPRHVLVLPLPAPTLSGIWVGLVTP
                     LPFTLTLPLVQSLQENAVTKDSDIDEVIPPPPDGLLGYEQSVRLALQREAEGAVDTNWA
                     AVAGGLDQAAKPLPNDPDWAGARVFTDDRTLHFPDLTPGQLWPVIEGIGGKNGWYSWPL
                     AWRVRGVWDKIVGGAGLNRGRRLPDRLRVGDPVDWWRVEELESGHRLLLRAEMRVSGRA
                     WLEFTISKAGGGSDLRQRAIFIPKGIRGQLYWAFVSPFHRFIFPAMAKNIARAAEKP"
     gene            1535..2434
                     /gene="crtB"
     CDS             1535..2434
                     /codon_start=1
                     /gene="crtB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: phytoene_synt"
                     /gene_kind="biosynthetic"
                     /product="phytoene synthase"
                     /protein_id="AAF65581.1"
                     /sec_met_domain="phytoene_synt (E-value: 4.9e-35, bitscore:
                     110.9, seeds: 8, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSSSPAYDVFSRTAQQAAKAVISNYSTSFGLATRLLGPSHRHHIR
                     NIYALVRIADEIVDGLAADAGLDTSEQHEMLENFVAATRAGLRHGVSDNLIIHSFAQTA
                     NATGITGDLIEPFFASMRADLETPSEPFAPVRGFDQDEHRAYVYGSAEVVGLMCLKVFL
                     CGQSRTPAQIEELEYGARQLGAAFQNINFLRDLADDSLRLGRSYLTAETRLTQTEKSAW
                     VTTIRQQLTNADDTIALLPKDARAAVRSAAALFTALNDKIADIPVDRLYRERVRIPNAL
                     KAQLTAKALLATAKENRA"
     gene            2431..4017
                     /gene="crtI"
     CDS             2431..4017
                     /codon_start=1
                     /gene="crtI"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222:dehydrogenase (Score: 484.9; E-value: 5.5e-147)"
                     /gene_kind="biosynthetic-additional"
                     /product="phytoene desaturase"
                     /protein_id="AAF65582.1"
                     /transl_table=11
                     /translation="MTERIGVIGGGAAGLATAALLAREGHSVDLFEQNSTLGGRIGSIE
                     EDGYRFDTGPSWYLMPEVYEHFFNLLGTTAANELDLRTLDPAYTVFSPASQARREQSVT
                     IPHGTAEIIDTFERIERGSGATLAKYLDSAKATKDLAIDYFLYNPFTSPKSILRAELLT
                     ALPALARLLTTSLERHASRSFHHPVLRQILQYPAIFLGTDPRKAPALYHLMSALDLSDG
                     VLYPMGGFRTIVDALTRLVEESGVRVRTDSTVTHISTVSESGRRRSANGIAWKDQDGRE
                     HFHDCDIVVSAADLHHTETQLLGEGERSYPESWWQKVTSGPGAVLVFLGVEGEVPQLPH
                     HSLFFTEDWSTNFDDIFGSQQKISSPASAYVCKPSHTDPNVAPFDCENLFILIPVPADA
                     GLGAGGDDGQGDPRIERAADQAIDQIAEWADIPDLRDRIRFRRTLGPTDFSSNYNSWLG
                     GMLGPAHTLRQSAMFRQQNASKKVDGLYYAGATTAPGVGVPMCLISAELVLKHIRGDSS
                     PGPLPVTDTRE"
     CDS             complement(4044..4856)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_kind="biosynthetic-additional"
                     /note="ORF4; similar to Rhodobacter sphaeroides sulfolipid
                     biosynthesis protein sqdC, PIR Accession Number C45729"
                     /product="hypothetical protein"
                     /protein_id="AAF65583.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 2.1e-08, bitscore:
                     23.4, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRVLLAGCGDLGTRLGLNLVRKGHEVIGLRRHTESLPASFTPFAV
                     DLTRPGTVKIDDIDAVVITLTPDEYTDDGYEQTYLRGVQGLIGVLESTPERVILLSSTR
                     VLGSASPGAVANEYSQPRPDSSPARTLWETENLIRDTFASALIIRAAGIYGRENARLIE
                     GVRSGRPVNYRRWTNRIHHADLVRALEALLFSSDAPRLMHAVDSCPVQLGEVVEFLASE
                     LNVSPPPDLSAEPTEGKRLENTRFHEFLGSLEFPTYREGYSHQLRTGA"
     gene            complement(4923..6386)
                     /gene="cpd1"
     CDS             complement(4923..6386)
                     /codon_start=1
                     /gene="cpd1"
                     /product="hypothetical DNA photolyase"
                     /protein_id="AAF65584.1"
                     /transl_table=11
                     /translation="MSTSGEDLTLVWFRDDLRVADHEALTAARADGRVIAVWIRETRDE
                     EGLGPRPLGGACRWWAHESLTVLHEELSDLGIPLLFAAGRAEKIIVQLASSLGVTAVRW
                     TRRYASASRALDGQIKTVLNDQGFTVHSHTGALLVEPWTAAPQSSDHYKVFTPFWKAVA
                     GRDVGDVLPKPQAQSPLSQTLLSRAHECPAVMELDGLGLLDGTETGAGEAAAASGPRWW
                     QDTIAGHWSPGLRAAEDQLDDLSAIIDGYTTSKDVPSDAASTSRLSPRLRHGELSPRQL
                     LQAARTTSSLTQDDRAAWIRQLYWREFSWHLTYHYPHIDSAPIRPEFHNFPYEDDDDAL
                     THWRAGTTGYPLIDAGMAQLWQTGWMHNRVRMVTASFLTKNLLQHWWFGEQWFWDTLVD
                     ADEANNPVSWQWVAGSGADAAPYFRVFNPERQRERFDPDDAYIEDWLPYTPATAPPPLV
                     DLRQSRQAALSAYDHMKNRSTTRDNPGES"
     gene            complement(6386..6904)
                     /gene="crtK"
     CDS             complement(6386..6904)
                     /codon_start=1
                     /gene="crtK"
                     /note="similar to Rhodobacter capsulatus crtK gene product,
                     SwissProt Accession Number P17057"
                     /product="hypothetical protein"
                     /protein_id="AAF65585.1"
                     /transl_table=11
                     /translation="MKAPWKRYLLPAAMCAAAAGIGAFGTRVDSDWYKSVTTPAWQPPG
                     WVFGPAWSTLYTLTAVASGRVLTRLPNSRDRRAYLGELGANMAVNVAWSWLFFSARRPD
                     ASLVDSLILEASTLRLLRKAAAVDKTAALMLTPYATWVGFATVLNGEILRLNPDEMPRS
                     LSCTRTAGR"
     gene            complement(6950..8504)
                     /gene="crtU"
     CDS             complement(6951..8504)
                     /codon_start=1
                     /gene="crtU"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222:dehydrogenase (Score: 45.1; E-value: 1.3e-13)"
                     /gene_kind="biosynthetic-additional"
                     /product="betacarotene desaturase"
                     /protein_id="AAF65586.1"
                     /transl_table=11
                     /translation="MTQRRRPRDRFAERIQGPQGRPRLLRPKRVTIIGAGIAGLAAAAI
                     LAEHGAEVTVIEKTDYLGGRVGAWPVDDERTMSRGFHAFFRQYYNLRDLLSRADPEGEC
                     LRPVDDYPLIHRRGSMDTFASIPRTPPFNLLGFVWQSPTFPIRGLRDVDIAAAVELIDV
                     EFPATYSYYDGESAADFLDRLRFPDEARHLALEVFARSFFADPTEFSAGELVAMFHTYF
                     TGSAEGLLFDVPVDDYDTALWAPLGGYLESLGVTIETGTTVTSIDPTESGWTTTTGEAN
                     LESDAVVLAVDPAAARDLLSASHDSLVDSAPAAQRWMETIGSQTNAPAFAVLRLWLGTP
                     VADHRPAFLGTSGYDLLDNVSVLERFEAGARAWSESHHGSVLELHAYALEGDSYDTERG
                     RADIVARLLSDLHHVYPETAALTIVDQELLIEADCGLTDTRPWEDRPEPSTPIPGLVVA
                     GDYVRCNTPVALMERAATTGYLAANHLLSTWRVEGTDLWSPPTRGLLRRGVLGLIRRRR
                     "
     gene            complement(8501..8824)
                     /gene="crtYd"
     CDS             complement(8501..8824)
                     /codon_start=1
                     /gene="crtYd"
                     /product="lycopene cyclase"
                     /protein_id="AAF65587.1"
                     /transl_table=11
                     /translation="MLPEYTVMTVVGIVIVIGLEIFVFRSGIFRRGQYWAALAICLAFQ
                     CLVDGWLTKLSDPIVRYNPSQFLNVRFPWDIPIEDFGFGFAMITSVLMLWQWQLNRSRK
                     DTQ"
     gene            complement(8814..9191)
                     /gene="crtYc"
     CDS             complement(8814..9191)
                     /codon_start=1
                     /gene="crtYc"
                     /product="lycopene cyclase"
                     /protein_id="AAF65588.1"
                     /transl_table=11
                     /translation="MSAFEYLLLMGACLLITLPLELLFSARVYRRPKLLIGSLIPIILV
                     FSLWDIIAIDRDHWTYNQQFVTGIHIGNLPLEELVFFIVIPICALLSYEAVGTVLKFVA
                     KKSGTRAGRKSGNRKDGGDVA"
     CDS             complement(9188..10417)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007:cytochrome P450 (Score: 151.7; E-value: 4.8e-46)"
                     /gene_kind="biosynthetic-additional"
                     /inference="non-experimental evidence, no additional
                     details recorded"
                     /note="ORF10"
                     /product="hypothetical protein"
                     /protein_id="AAF65589.1"
                     /sec_met_domain="p450 (E-value: 1.1e-16, bitscore: 50.4,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNSVSMRKAVGTHDETEPRVGHDGTMWRIRSYEAATTVLRARHQT
                     TQAGFTAEKIPRGYFRQHPILISDGDDHDVQRREVARYFAPAVVSAKYGDFINKRAQAL
                     VDSALANGRLRLDEAALNYSVEVTAEIVGLTDSPIDAMAKRLVGFFRQPPVDLAAPAMG
                     RTRRQWIQAAVNGLVPIGRFYISDVRPAIRARRDRRRDDVLSHLLSAGYSTADILVECV
                     TYGTAGMVTTREFITMACWHLLTNDELGRRYTEASQPTRLEILEEIIRLDPVVGHLYRR
                     AQADIEVSDNGCPYTIPEGDLIDVCVRQTNTDPQAMGAAPETVIPGRDLRRGSAATGLS
                     FSDGAHTCPGQPLALYETDALLHKVLATRPRILREPTISWDNVIEGYRLRGLDLSLMTR
                     SRPVTTRMTT"
     gene            complement(10414..11571)
                     /gene="crtE"
     CDS             complement(10414..11571)
                     /codon_start=1
                     /gene="crtE"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182:Polyprenyl synthetase (Score: 217.8; E-value:
                     2.9e-66)"
                     /gene_kind="biosynthetic-additional"
                     /product="GGPP synthase"
                     /protein_id="AAF65590.1"
                     /transl_table=11
                     /translation="MRLPGLSALGWSNTSLSSSRSLPNTSSKLKTMTDSATPIGDIAFS
                     FEDRLAQVLAEARKRSRTVSAQYGQLWDSLTHMATGGKLIRPRMLLDAHAALGGRDEHA
                     AVDAACAMQLLHIALVIHDDVIDNDTIRRGEATISGEFASEAMSRGALRRDANAWGIAT
                     SILGGDLILTLSHSLLARLDIEDTKRRAILDIFDDTVVESAAGEHSDVWLSLHLEGANS
                     PDVLAMVDQKTAVYSFQAPLLIAAVLAGADSGLIDDLANISRQIGVIYQLRDDVLGLFG
                     DERETGKSNVSDLREGKETLLITFARSDPSWPEVHTLFGDRMLSNADGDRLRKVIEESG
                     ALVFVESMITQRCENLYRLIGEAALPPALSNQLIQLTSDASSRTA"
     gene            complement(11471..12019)
                     /gene="idi"
     CDS             complement(11471..12019)
                     /codon_start=1
                     /gene="idi"
                     /product="isopentenyl diphosphate isomerase"
                     /protein_id="AAF65591.1"
                     /transl_table=11
                     /translation="MEMVVLVDHAGDPIGVQSKADVHGQSTPRHLAFSCHVVDEQGRLL
                     VTRRALKKRTWPGVWTNSFCGHPAPGETLEDAVSRRAEFELGLSIDHITCAIPNFSYVA
                     RDASGIEENEHCPVFIARAVSSLTPNPAEVAEAQWTSSSELKSAIDAAPWAFSPWLVEH
                     FTELQPLIAEHIVEAEDDD"
     CDS             12190..13446
                     /codon_start=1
                     /note="ORF13; similar to Mycobacterium tuberculosis
                     hypothetical protein Rv2807, e|276809"
                     /product="hypothetical protein"
                     /protein_id="AAF65592.1"
                     /transl_table=11
                     /translation="MEGKIPMTARRHVTNKLRTTYRKASKTDRSRILDEVMATTGMGRS
                     TARRMLTGPRLPDPGEHVDKRRLRPKTYSDASRVLLEHVWALMGMPCGKYFVVMLPMWL
                     PLLEQAGDLDHPFVSATAIEELESMSAATIDRYLAPARQSMQLRGISTTKPPPLLRNSI
                     GLSKTGDEPPTVAGVIEADTVAHCGPSYVGEFARTLTMTDMVTGWTENASIRNNASKWI
                     LEAVADLDGKFPFELRVFDSDNGSEFINHEVADWLQQRDIDQTRSRPYRKNDQATVESK
                     NNHVVRKHAFYWRYDTSEELGLLGQLWPLVSLRLNFFVPTKKPVEYATTSDGRRRRVYD
                     SPRTPWRRVLDSGLLTDDQVTAISERVDGVNPADLTRQINQIQMRLIELSKSKTEAMAA
                     SRHLDMASLKPSMNRLQTN"
     CDS             13472..14167
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1135:MarR family
                     transcriptional regulator (Score: 70.1; E-value: 2.4e-21)"
                     /gene_kind="regulatory"
                     /note="ORF14; similar to MarR family transcription
                     regulator, e|1182831"
                     /product="hypothetical protein"
                     /protein_id="AAF65593.1"
                     /transl_table=11
                     /translation="MLRHQHGSRAHSSVRHLAVERGIRPDSDLNYFLNEPEFSGRSHLS
                     RAEISQCNSIMEALRRWREADRALSEASRRYMHLNDSDMRAIRMLIHAQKQGRVVTPKD
                     IARDVGISSASTTKLIDRLVAGGHLSRSPHAKDRRTICIEVTPETARAARETIGRQHAR
                     RFDAAAAMSSEEREVVVRFLSALVEADQPQGDLAVPGQVSSGGSASRTEDQQPRRGFGE
                     SETSADSEG"
ORIGIN
        1 tgtctcgccc ttccacaggt tcatctttcc agccatgggt ggatcaggtc gaggtccatc
       61 gtgttgatct tgacgatggg gacggtctcg tcgaggccgc caccggggtc gacgtcatcc
      121 atcatctcgt tcactcgatg ggctcggggc acgacttcga gaagaaggag gcggcgacgg
      181 cccgtcgggt cgccgaggcg gccgaggctg ccggggttcg acgcatcgtc tacctcggcg
      241 ggctccatcc cgacgaggcg gacctgtcca tgcacatgag atctcgggct catgtcggac
      301 gtattctcct cgactcgcca gtgccgacga ttgcgtttca ggcgggcatc atcatcgggt
      361 cgggatcggc ttcctttgaa atggttcgtc acctcgccat gacgctgacg ctgatgcctg
      421 caccgaattg ggtcaacaac cgtgtcgaac ccctggccgt gcgtgatgtc ctctactacc
      481 tactcaaggc cgtcgacctc gatgaagacg tcaaccgcgc cttcgatatc ggatcacatg
      541 atgtccacaa atacgcggac atcatgaaga ggttcggtgc aattgctggc ctcaaacccc
      601 ggcatgtcct cgtcctgccg ttgcctgctc cgaccttgtc gggcatctgg gtcggtctcg
      661 tcaccccgtt gccattcacg ttgaccctgc cgctcgtcca atcgctgcag gagaacgctg
      721 tgaccaagga cagcgacatc gacgaggtca ttccccctcc ccccgatggt ctgctcggct
      781 acgaacagtc cgtgcgcctg gccctccagc gtgaagccga gggtgctgtc gacaccaatt
      841 gggccgccgt ggctggagga ctcgaccagg cggcgaagcc cttgcccaat gatcccgatt
      901 gggcaggagc acgcgttttc accgatgatc ggacactgca ttttcctgat ctgactcctg
      961 gacagctctg gccggtcatt gaaggcattg gtgggaaaaa tggttggtac tcgtggccgt
     1021 tggcatggcg ggtacgggga gtatgggaca agatcgtcgg cggtgccggc ctcaaccgtg
     1081 gacggcgact accggaccgt cttcgagtcg gcgaccccgt tgactggtgg cgagtcgaag
     1141 agcttgagtc ggggcatcgc ctgctgctga gagcagaaat gcgggtatct ggtagggcat
     1201 ggcttgaatt caccatcagc aaggccgggg gtggcagcga cttgaggcaa agggcgatct
     1261 tcattcccaa gggcattcgc ggccagctgt attgggcgtt tgtctcgccc ttccacaggt
     1321 tcatctttcc agccatggcg aagaacatcg ctcgggctgc agaaaagccc tgacaatcaa
     1381 ggtcgcaagc tgtttcgtta gctaagctag cagttatgtt cttcacacgc agccgctgca
     1441 gcgccagtgg tgtcaatgat gtctcgactc gccttgccca cattcgcttc aacgccgcca
     1501 tggcaggatc agagtcggtc ggaggtgctg cgcggtgagc agctcacctg cctacgacgt
     1561 cttcagccga acggcgcaac aggcagccaa ggccgtcatc tcgaactatt cgacatcgtt
     1621 cggactggct acccggctgc tcggaccttc tcaccgtcat cacatccgca atatctacgc
     1681 tctcgtccga atcgccgatg agatcgttga cggtcttgcc gccgatgcgg gcctcgacac
     1741 gagcgagcag catgagatgc tcgagaactt cgtcgccgcg actcgcgctg gactcagaca
     1801 tggcgtcagc gacaacctca tcatccactc cttcgcccag accgcgaacg cgacgggaat
     1861 caccggcgat ctcatcgaac ctttcttcgc ctcgatgaga gcggacctgg aaacccccag
     1921 tgagcctttc gccccggtcc gcgggttcga tcaggacgaa caccgggcct acgtctacgg
     1981 ctccgccgag gtggtcggtc tcatgtgcct caaggtcttc ctgtgtggtc aatcccgaac
     2041 accggcccag atcgaggagc tcgaatacgg tgcccgtcag cttggagccg cattccagaa
     2101 catcaacttc ctccgcgact tagccgacga cagtctgcga cttggccgca gctacctcac
     2161 cgctgaaacc cgactcaccc agaccgagaa aagcgcatgg gtgacgacga ttcgccagca
     2221 gctgaccaac gccgacgaca cgattgcact gctgccaaaa gatgccagag ccgcggtgag
     2281 aagtgccgcg gcactcttca ccgcactcaa cgacaagatc gcagacatcc ccgtcgaccg
     2341 tctctatcgg gagcgtgtgc gcatcccaaa cgccctcaaa gctcaactca cggcgaaagc
     2401 gctgctggcc acggcgaagg agaacagagc atgactgagc gcatcggcgt catcggcgga
     2461 ggtgcggcag gactggctac ggcagcactg ttggcacgtg agggccacag cgtcgatctc
     2521 tttgaacaga actcaactct gggcgggcgg atcggttcga tcgaagaaga cggctaccga
     2581 ttcgacacag gaccatcgtg gtatctgatg ccggaggtct acgaacactt cttcaacctt
     2641 ctcggtacga cggcggccaa cgagctcgac ctgcgcacac tcgatcctgc ctacaccgtg
     2701 ttcagtcccg cttcgcaggc tcggcgggaa cagtcggtca cgattcctca cggcacggcc
     2761 gagatcatcg acaccttcga gcgaatcgaa cgcggcagcg gggccacctt ggccaaatat
     2821 ctggactcgg ccaaggccac caaagactta gcgatcgact acttcctgta caaccccttc
     2881 acctccccga aatcgattct cagggcagag ctgctcaccg cgctccccgc gctggctcgg
     2941 ctgttgacca cctctctgga acgccacgcc tctcgctcat tccaccatcc ggtgctgagg
     3001 cagatcctcc agtacccggc catcttcctt ggcaccgacc cgaggaaggc gccggccctc
     3061 tatcacttga tgagtgccct cgacctcagc gatggagtgc tgtacccgat gggagggttc
     3121 aggaccatcg tcgatgctct gacccgactt gtcgaggaat caggggtgcg agtgcgcacc
     3181 gactccaccg tcacacacat atccaccgtg tcagaaagtg gccgacgacg atctgccaac
     3241 ggcatcgcat ggaaggatca agacggtcgc gaacactttc acgactgtga catcgtcgtc
     3301 tccgccgctg acctccacca cacggagacg cagctcttgg gcgagggtga acgcagctac
     3361 cccgagagct ggtggcagaa ggtgacaagc gggccaggcg ccgtcctggt ctttctcgga
     3421 gtcgaaggcg aagtcccaca gcttccccac cactcattgt tcttcaccga agactggtcg
     3481 acgaacttcg acgacatctt cgggtcgcag cagaagatct cctctccggc gtcggcatat
     3541 gtgtgcaaac ccagtcacac cgatccgaac gtggcgccat tcgactgcga gaatctcttc
     3601 attctcatcc cggtgccggc agatgcagga ctcggggcgg ggggagacga cgggcagggg
     3661 gatccgcgaa tcgagcgtgc agccgaccag gccatcgacc agatcgctga atgggccgac
     3721 atccccgacc tgagagatcg gatcagattc cgccgcacat tggggccgac ggatttctcc
     3781 agcaactaca attcgtggct gggagggatg ctcggaccag ctcacacctt gcgccagagc
     3841 gcaatgttcc gccagcagaa tgcctccaaa aaggtggatg gactctacta cgcaggtgcg
     3901 acgacggcgc cgggggtggg cgtgccgatg tgtctgatca gcgctgagct ggtcctcaaa
     3961 cacattcggg gggattcgag tccgggaccg ctgccggtca cagacacacg ggagtaaaga
     4021 ccgactgtca gtcgatgacc ggcttatgct ccggtgcgca gctgatgaga atatccctct
     4081 cggtacgttg gaaattccaa ggatccgagg aactcgtgga atcgggtgtt ctcgagtctc
     4141 ttaccctctg tgggttccgc ggagaggtct ggcggaggcg agacgttgag ctcggatgcc
     4201 agaaattcga cgacttcgcc cagctgcacg gggcacgagt ccacggcgtg catcagccgt
     4261 ggagcatcag agctgaagag aagagcctcg agcgcccgga ccagatcggc atggtggatg
     4321 cggttggtcc accgtcggta gttcacaggc cgacccgagc ggaccccctc gatgagtcga
     4381 gcattctccc gcccgtagat gcctgctgcc cgaatgatca acgcagacgc gaaggtgtcc
     4441 ctgatgagat tctcggtctc ccagagagta cgggcgggac tcgagtcagg gcggggttgg
     4501 gagtattcat tcgcaacagc gcccggcgat gcactcccga ggactcgagt cgaggacagg
     4561 aggatcacac gctcaggcgt ggattccagg acaccgatca ggccctgaac cccgcgcaga
     4621 tacgtctgtt cataaccgtc atcggtgtac tcatcggggg tgagagtgat caccactgca
     4681 tcgatgtcat cgattttgac cgtgccgggc cgggtgagat cgaccgcgaa gggagtgaac
     4741 gaggcgggaa gtgactcagt atgtcgccgc agtccgatga cctcatgacc cttgcgcacc
     4801 agattcaggc ccaaacgggt tccgagatct ccgcatcctg ccagcagtac tctcatgttc
     4861 gacttgccat cctgtcgtcg tcgtgtgtga gccggggaac ctgtttcctt tggttcacca
     4921 tgtcaggact ccccggggtt gtcgcgggtt gtggacctgt tcttcatgtg gtcgtaggca
     4981 ctcagggctg cctgccgtga ttgcctgaga tcgacgaggg gaggcggagc tgtcgccgga
     5041 gtgtagggga gccagtcctc gatgtaggcg tcgtcggggt cgaagcgttc gcgttgtcgc
     5101 tccgggttga agactcggaa atacggcgcg gcatcggcac cgcttccggc gacccactgc
     5161 catgagaccg ggttgttggc ttcatcggcg tcgacgaggg tgtcccagaa ccactgttct
     5221 ccaaaccacc aatgctggag gagattcttg gtcaggaagc ttgccgtgac catgcggacc
     5281 cgattgtgca tccacccggt ctgccacagc tgtgccattc ccgcatcgat gagcggatac
     5341 ccggtggtcc ccgctctcca gtgggtgaga gcatcgtcat catcttcgta gggaaagttg
     5401 tggaactcgg gtctgatcgg cgcagagtcg atgtggggat agtggtaggt caggtgccag
     5461 gagaactctc gccagtagag ttgacggatc catgccgcgc ggtcgtcctg ggtgagcgaa
     5521 ctggtcgtcc gagcggcctg gagcagctgt cgcggggaca gttctccgtg ccgcagcctg
     5581 ggcgagagcc gcgaggtgct cgccgcgtca gagggcacat ccttgctggt ggtgtagccg
     5641 tcgatgatcg cggacaggtc atcgagctgg tcttcagccg cgcgcaggcc cggcgaccag
     5701 tggccggcga tggtgtcctg ccaccagcgg gggcctgagg ctgcggcggc ttcacccgct
     5761 cctgtctctg tgccgtcgag gagtcccagc ccgtcgagtt ccatcaccgc cgggcactca
     5821 tgcgctctgc tgagcagtgt ctggctcagc ggtgattgtg cctgcggctt cggcagcacg
     5881 tcaccgacat cgcgtccggc aacggccttc cagaacgggg tgaagacctt gtagtggtca
     5941 ctgctctgcg gggcagccgt ccacggttcg acgaggaggg ctccggtgtg ggaatggacc
     6001 gtgaagccct gatcgttgag cacagtcttg atctggccgt cgagagcacg ggaagcggat
     6061 gcgtagcggc gcgtccagcg aacagcggtg acgccgaggc tgctggccag ctgcacaatg
     6121 atcttttccg cccggcccgc ggcgaagagc agaggaatgc cgaggtcgga gagctcctcg
     6181 tggagcacgg tcagcgattc gtgtgcccac caccggcaag ctccgcccaa gggcctgggc
     6241 cccagaccct cctcgtcgcg ggtctcacgg atccagacgg cgatgacgcg tccatcggca
     6301 cgcgccgcgg tgagggcctc atggtcggcc acccgcagat cgtcacgaaa ccacaccaga
     6361 gtgaggtcct caccgcttgt gctcatcagc gacctgctgt ccgggtgcaa ctcagagatc
     6421 gaggcatctc atctggattg agtctgagga tctctccatt gagaacagtc gcgaacccca
     6481 cccaggtcgc atagggggtg agcatgagcg ctgcagtctt gtcgacggca gccgccttcc
     6541 tcagcagcct cagcgtcgag gcctctagga tcagggagtc gaccagcgac gcatccgggc
     6601 gacgggcgga gaagaacagc cagctccatg cgacattgac cgccatgttc gcgcccaact
     6661 cgcccaggta ggcgcggcgg tcccggctgt tgggcaggcg cgtgagcacc cgtccggagg
     6721 cgactgccgt caacgtgtac agggtcgacc atgcgggtcc gaagacccag cccgggggct
     6781 gccacgccgg tgtggtgaca cttttgtacc agtcggagtc gactctcgtc ccgaatgccc
     6841 cgattcctgc tgccgcagcg cacatggcgg ccggaaggag atagcgtttc cacggagctt
     6901 tcatgggttc ctctcgatga ttcgcagtga tggtcttcga cggggcatgg tcatcgacgt
     6961 ctcctgatga gcccgagcac tccacgccga agcaggcctc gggtcggtgg cgaccacagg
     7021 tccgtcccct cgaccctcca ggtagagagc aggtggttgg cggccagata accagtcgtg
     7081 gcggcacgtt ccatcaaggc cacaggggta ttgcagcgca catagtctcc ggcgaccacc
     7141 agcccgggga tcggggtgga cggctcgggc ctgtcctccc acgggcgggt gtcagtaaga
     7201 ccgcagtccg cttcgatgag cagctcctgg tcaacgatgg tcagggctgc ggtttcgggg
     7261 tagacgtgat gcagatctga cagaagccgc gcaacgatgt ccgccctccc acgctcggtg
     7321 tcgtatgaat cgccttcaag ggcataagcg tggagttcga ggaccgaacc gtggtgggat
     7381 tcggaccacg ctctggctcc ggcctcgaag cgctcaagta cggacacgtt gtcaaggagg
     7441 tcgtacccgc ttgtccccag gaaggccggt cggtggtcgg ccacgggcgt gccgagccac
     7501 agtcgcagca ctgcgaacgc gggagcgttg gtctgtgagc cgatcgtctc catccaccgt
     7561 tgggccgcgg gtgcgctgtc cacgagcgag tcatggcttg cgctgagcag atcgcgggca
     7621 gcggcaggat cgactgcgag cacgacggca tcactttcca ggttcgcctc tccggtcgtg
     7681 gtcgtccatc cggactcggt gggatcgatc gaggtcacgg tcgtccccgt ctcgatcgtg
     7741 acccccagtg actcgaggta gccgcccaac ggtgcccata gagctgtgtc gtagtcatcg
     7801 acggggacgt cgaagagcag cccttccgct gaaccggtga agtaggtgtg gaacatggcc
     7861 acgagctcac ccgcagagaa ctctgtcggg tcggcgaaga aggagcgggc gaagacttcg
     7921 agcgccagat ggcgggcttc gtcgggaaag cgcaaccggt cgaggaagtc ggcggcagat
     7981 tcgccgtcat agtagctgta cgttgcgggg aactcgacgt cgatgagttc gactgcggca
     8041 gcgatatcga cgtcgcggag tcctctgatc gggaaggtgg ggctctgcca gacgaaaccg
     8101 aggagattga acggcggggt gcggggaatt gaggcgaacg tgtccatcga gcctcggcga
     8161 tggatgagcg ggtagtcgtc gacgggccgc aggcattcac cttcgggatc tgcgcggctg
     8221 agcaggtcgc gcaggttgta gtactgccgg aagaaggcgt ggaatcctcg gctcatggtc
     8281 cgttcgtcgt cgaccggcca ggcgcccact cggccgccga ggtagtcggt cttctcgatg
     8341 accgtgacct cggcgccgtg ttcggccaaa atcgcggctg cagccagtcc ggcgatgccg
     8401 gcgccgatga tggtgacccg tttgggtcga agcagtcgcg gccgtccctg cgggccctgg
     8461 attctctcgg cgaagcgatc tctgggccgg cgtctctgtg tcattgggtg tccttacgcg
     8521 atcggttgag ttgccactgc cacagcatga gcacagatgt gatcatggcg aacccgaaac
     8581 cgaagtcttc gatcgggatg tcccagggga aacgcacgtt cagaaactgc gacgggttgt
     8641 acctgacgat gggatcactg agtttggtca gccacccatc gaccaggcac tggaaggcca
     8701 gacagatggc cagcgctgcc cagtactggc ctcgtcggaa gatcccgctg cggaagacga
     8761 agatctcaag accgatgacg atgacgatgc cgaccaccgt catgacagta tattcaggca
     8821 acatcgcctc cgtccttccg gttcccggac tttctgccag ccctggttcc ggacttcttg
     8881 gccacgaatt tgagtacagt gccgaccgct tcgtagctga gcagggcgca gatcggaatg
     8941 actatgaaga agacaagctc ctccagcggc aggttgccga tgtgaatgcc ggtgacgaac
     9001 tgctggttgt acgtccaatg gtctcggtca attgcaatga tgtcccagag ggagaagacg
     9061 aggatgatcg ggatgagaga tccgatgagg agctttgggc gacggtagac ccgggcggag
     9121 aacagcagtt cgagaggaag cgtaatcagc agacaggcgc ccatcagcag caggtattcg
     9181 aaggcgctca tgttgtcatt ctcgttgtga cagggcgtga tctcgtcatc agactcaggt
     9241 ccaagccacg cagacggtat ccttcaatga cgttgtccca gctgatggtg ggctcccgaa
     9301 ggatgcgtgg gcgagtagcc aggaccttgt gcagaagggc atcggtttcg tagagagcca
     9361 atggttgacc cgggcacgtg tgggctccgt cgctgaagct cagccctgtc gcggcgcttc
     9421 cccgtctcag gtcgcgccca gggatgaccg tctccggggc tgcccccatc gcctgcgggt
     9481 cggtgttggt ctgtctcacg cacacatcga tcaggtcgcc ttcggggatg gtgtaggggc
     9541 agccgttgtc tgagacttcg atgtcggctt gggcccgccg gtagaggtgg ccgacgactg
     9601 ggtcgaggcg gatgatctct tcgaggatct ccaggcgggt gggctgcgag gcttcggtgt
     9661 agcgacggcc aagctcgtcg ttcgtcagca gatgccagca tgccatggtg atgaattcgc
     9721 gagtggtcac catgccggct gtgccgtagg ttacgcattc gaccaggatg tcagcggtgc
     9781 tgtaaccggc ggacagcaga tgcgagagca catcgtcgcg gcgtcggtcg cgccgggcgc
     9841 gaatcgccgg gcgcacatcg ctgatgtaga accggccgat gggcacgagg ccgttgacgg
     9901 ccgcctggat ccattggcga cgcgttcggc ccatcgcggg ggcggcgagg tcgacaggtg
     9961 gctgtctgaa gaacccgacg agacgcttgg ccatcgcgtc gatgggggag tccgtgaggc
    10021 ccacgatttc ggctgtgact tcgaccgaat agttcagagc tgcctcgtcg aggcgcagtc
    10081 gaccgtttgc gagggcggag tcgacgaggg cttgagcacg cttgttgatg aaatccccgt
    10141 acttcgcaga caccacggcg ggggcgaagt agcgggcgac ttctctgcgc tgtacatcgt
    10201 gatcgtcgcc gtcggagatc agtatggggt gttgccgaaa gtacccgcgc gggatctttt
    10261 cggcggtgaa ccctgcctga gtagtctgat gacgcgcacg cagcacagtt gtggcggcct
    10321 cgtaggagcg tatcctccac atggttccgt cgtggccgac tcggggttcg gtctcatcat
    10381 gtgtccccac cgcctttcgc atgctcaccg agttcatgcg gtgcgtgagc tggcgtcgga
    10441 ggtcagttgg atcagctggt tgctcaacgc tggcgggagg gccgcctcgc cgatgagccg
    10501 atacaggttc tcgcagcgct gggtgatcat tgattcgacg aacacgagtg ctcctgattc
    10561 ctcgatgact ttccgcagcc gatcaccatc ggcgttgctc agcatcctat ccccgaagag
    10621 ggtgtgaacc tctggccagg acggatcgga gcgagcgaac gtgatcaaga gggtctcctt
    10681 gccctcacgg aggtcactga cattggattt ccccgtctcc cgttcgtcac cgaacagtcc
    10741 gaggacatcg tcgcgcagct ggtagatcac accgatctgc cgggagatgt tcgccaaatc
    10801 gtcaatgagg ccactgtcgg cacctgccag cacggccgcg atgagcagcg gtgcttgaaa
    10861 cgaatacact gcggtcttct gatcgaccat ggccaggacg tctggagagt tcgccccttc
    10921 gagatgcagg ctcaaccaca catcggagtg ttcaccggcg gcactctcaa cgactgtgtc
    10981 atcgaagatg tcgaggatgg cccgtcgttt ggtgtcctcg atgtcgaggc gggcgaggag
    11041 cgaatgggag agggtgagga tgagatcacc gccaagaatc gatgttgcga tgccccacgc
    11101 attggcatcg cgtcgcagcg cgccgcgcga catggcttcg gacgcgaact ccccggagat
    11161 cgtcgcttca ccgcgacgga tcgtgtcatt gtcgatcacg tcatcgtgga tgacgagggc
    11221 gatgtgcagc agctgcatgg cacaggctgc gtcgacggcg gcatgttcgt ctctgccgcc
    11281 cagggccgcg tgcgcgtcga gcagcattcg tggccggatc agctttccgc ccgtggccat
    11341 gtgtgtgagt gagtcccaga gctgtccgta ttgtgcagag acggtgcgag accgcttccg
    11401 tgcctccgcg agcacttgtg cgagccggtc ctcgaacgag aatgcgatgt caccgatggg
    11461 agtggccgag tcagtcatcg tcttcagctt cgacgatgtg ttcggcaatg agcggctgga
    11521 gctcagtgaa gtgttcgacc agccaagggc tgaaagccca gggagccgca tcgatcgccg
    11581 acttcagttc tgaagagctc gtccactgag cctcagctac ctcggcgggg ttcggtgtca
    11641 gtgacgatac ggctcgggcg atgaagacag ggcagtgctc gttctcttcg attcccgaag
    11701 catcacgggc cacgtagctg aaattgggga tggcgcaggt gatgtggtcg atgctcagac
    11761 cgagttcgaa ctcggcgcgt cggctgactg cgtcctcaag cgtttcaccg ggggcagggt
    11821 gaccgcagaa ggaattcgtc cagactccag gccatgtccg cttcttgagc gcgcgccgtg
    11881 tgaccaggag gcgaccctgt tcgtcgacga cgtgacacga gaacgccaag tgtcgaggag
    11941 ttgactggcc atggacatcg gccttgctct ggacgccgat aggatcaccc gcgtggtcga
    12001 cgagaacaac catttccatt gatggacacc actttcgtta gccaggcgag agttcaatct
    12061 agcatagcaa cggtattctg tgttcgtgtc aaccgtcgac gtgcctcagc tattttgagc
    12121 gcgtagaacc aggcggtgcc tcaaagactt tgagcgcgaa ctcccaacaa acgggaacag
    12181 gcttgcccta tggaaggcaa gatcccgatg accgccagac gccacgtgac gaacaaactc
    12241 cgcaccacct accgcaaagc ctcgaagacc gaccggtctc gcatcctcga cgaagtgatg
    12301 gccacgaccg gaatgggccg gtcgacggcc aggagaatgt tgaccggtcc ccgcctaccg
    12361 gacccgggcg aacacgtcga caaacgacga ctcaggccca aaacctactc cgatgcctca
    12421 cgcgtcctcc tcgagcacgt gtgggcgctg atgggcatgc cctgcggaaa gtacttcgtc
    12481 gtgatgctgc cgatgtggct gcccctgctc gaacaagcag gagacctcga ccatccattc
    12541 gtctctgcga ctgcgatcga ggaactggaa tcgatgagcg ctgctaccat cgaccgatac
    12601 ctggccccgg ccaggcaatc gatgcagctg cgaggcatct cgacgacgaa gccgccacca
    12661 ctgctacgca actcgatcgg actgtcgaag accggcgatg aaccaccgac cgtggccgga
    12721 gtcatcgagg ccgataccgt cgcccactgc gggcccagct acgtcggtga gttcgcccgg
    12781 accttaacga tgaccgacat ggtcaccggg tggaccgaga acgcctcgat ccgcaacaac
    12841 gcctcgaaat ggatcctcga agcggtggct gatctggacg ggaagttccc gtttgaactg
    12901 cgtgtcttcg attccgataa cgggtccgag ttcatcaacc acgaagtcgc cgactggctc
    12961 caacaacgtg atatcgacca gacgaggtcg cggccctaca ggaagaacga ccaagcgacg
    13021 gtggagtcga agaacaacca tgtcgtacgc aaacacgcct tctactggcg ctatgacacc
    13081 agcgaagaac tggggctgct gggccagtta tggccgttgg tgtcgctgcg gctgaatttc
    13141 ttcgtcccga cgaagaagcc cgtcgaatat gccaccacca gcgatggtcg tcgccgacgt
    13201 gtctacgaca gtccccggac cccgtggcga cgagtcctcg attccggact cctgaccgat
    13261 gaccaggtca cggccatcag cgaacgagtc gatggtgtca atcctgccga tctgactcgc
    13321 cagatcaatc agatccagat gcgattgatc gagctgtcga agtccaagac cgaggccatg
    13381 gctgccagtc ggcacctgga catggcatca ttgaaaccgt cgatgaatcg actccaaacg
    13441 aattagtgaa ccgtaagcct cacgctcaaa aatgctgagg caccaacacg gatcacgcgc
    13501 tcactcctca gtgaggcacc tcgccgtaga gagaggaatc cgcccggatt cagatctgaa
    13561 ctatttcttg aacgagccgg aattctccgg tcgttctcac ttgtcgcggg ccgaaatcag
    13621 tcaatgcaac tccatcatgg aagctctgcg ccgttggcgc gaagctgatc gggcactgtc
    13681 tgaggcatcc aggcgctata tgcacctcaa cgacagtgac atgcgcgcca tccggatgct
    13741 catccacgct cagaagcagg ggcgggtcgt caccccgaag gacatcgccc gtgatgtcgg
    13801 gatctccagc gcgtcgacga cgaagctcat cgaccgtctt gtcgcgggtg gccatctgag
    13861 tcgctcaccg cacgccaaag atcggcggac gatatgcatc gaggtgacgc cggagacggc
    13921 ccgagcggca cgggagacga tcggccgcca gcacgcacgc cgctttgatg ccgccgcagc
    13981 aatgagttcc gaagaacgcg aggtcgtcgt ccgattcctc tcagctctgg tagaagctga
    14041 ccaaccgcag ggcgacctgg ctgtcccagg gcaggtgtcc agtggcggtt cagccagcag
    14101 gactgaggat caacaacctc gacgaggatt cggggaatca gaaacgtccg ctgactcgga
    14161 agggtgatct gcgttgccgt cgaggtaacc gtgcacttca cgtatgtgag gagatgtggt
    14221 caccgtggta gaactcagca gtgccggagt gccgaagaag tcggagctcc cggcgacctt
    14281 ggggcgctcg gatgccgaag ccctcgccgg ggccatccga tgcgcaggcc acgggcggga
    14341 agaacaccgc tcgcccagcc gcatgtgggg tggacgtcac tgcctgcccc gcgttacc
//
