LOCUS       BGC0000637              9233 bp    DNA     linear   BCT 09-JUL-2001
DEFINITION  Brevibacterium flavum carotenoid biosynthesis gene cluster, partial
            sequence.
ACCESSION   BGC0000637
VERSION     BGC0000637
KEYWORDS    .
SOURCE      Corynebacterium glutamicum (Brevibacterium flavum)
  ORGANISM  Corynebacterium glutamicum
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Corynebacteriaceae; Corynebacterium.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 13:58:07
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..9233
                     /db_xref="taxon:1718"
                     /mol_type="genomic DNA"
                     /note="synonym: Brevibacterium flavum"
                     /organism="Corynebacterium glutamicum"
                     /strain="MJ233"
     protocluster    1..9233
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="True"
                     /core_location="[3913:4828]"
                     /cutoff="20000"
                     /detection_rule="(Terpene_synth or Terpene_synth_C or
                     phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7
                     or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      3914..4828
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(Terpene_synth or Terpene_synth_C or
                     phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7
                     or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
     cand_cluster    1..9233
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Terpene_synth or Terpene_synth_C or
                     phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7
                     or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..9233
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000637"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..9233
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="terpene"
                     /region_number="1"
                     /rules="(Terpene_synth or Terpene_synth_C or phytoene_synt
                     or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps
                     or fung_ggpps2 or trichodiene_synth or TRI5)"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            541..1689
                     /gene="crtE"
     CDS             541..1689
                     /codon_start=1
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /gene="crtE"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182:Polyprenyl synthetase (Score: 180.2; E-value:
                     8.2e-55)"
                     /gene_kind="biosynthetic-additional"
                     /product="GGPP synthase"
                     /protein_id="AAK64296.1"
                     /transl_table=11
                     /translation="MRHNWPCDTRMDNGMTITTEHSTHPDLDFNDEIYRELNRICASLS
                     QQCSTYPPEFRTCLDAAFQALRGGKLIRPRMLLGLYDALVDDDIEVKLNTVLQVAAALE
                     LLHFSLLVHDDVIDGDLYRRGKLNFIGQILIHRTPESFAQTQRDPEHLDWAQSNGLLMG
                     NLFLAATHQIFARLDLPHPQRVRLLDLLNHTMNDTIVGEFLDVGLSSKAISPNMDIALE
                     MSRLKTATYSFELPMRAAAILAELPQEIETQIGEIGTNLGIAYQLQDDYLSTFGDAAEH
                     GKDAFSDLREGKETTIIAYARNTAKWNDIEDIFGSADLSTSQAERIQHLLIQCGAKNHS
                     LNAISDHLNICRSMIETLSTQVYPKAQKLLLKHVEQLASRKS"
     CDS             1725..3917
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1035:MMPL domain-containing transport protein (Score:
                     522.9; E-value: 2.1e-158)"
                     /gene_kind="biosynthetic-additional"
                     /note="orf4; similar to Streptomyces coelicolor actII-ORF3"
                     /product="unknown"
                     /protein_id="AAK64297.1"
                     /transl_table=11
                     /translation="MSTSITTERRKKSGSTRLMRIFLPALLILVWLVGAGIGGPYFGKV
                     SEVSSNSQTTYLPESADATQVQEQLGDFTDSESIPAIVVMVSDDPLTQQDIAQLNEVVA
                     GLSALDIVSDEVSPAIPSEDGRAIQVFVPLSPSAELTESVEKLSETLAQQTPDYVSTYI
                     TGPAGFTADLSAAFAGIDGLLLAVALAAVLVILVIVYRSFILPIAVLATSLFALTVALL
                     VVWWLAKWDILLLSGQTQGILFILVIGAATDYSLLYVARFREELRVQQDKGIATGKAIR
                     ASVEPILASGSTVIAGLLCLLFSDLKSNSTLGPVASVGIIFAMLSALTLLPALLFVFGR
                     VAFWPKRPKYEPEVVHGENGIPASGIWSKVAHLVEQHPRAIWVSTLIVLLLGAAFVPTL
                     KADGVSQSDLVLGSSEARDGQQALGEHFPGGSGSPAYIIVDETQAAQVADVVLNNDNFE
                     AVTVTSADSPSGSAPITADGIVPLDSGTAPAPVVVEGQVLLQATLVEAPDSEEAQKAIR
                     SIRQTFANENISAVVGGVTATSVDTNDASIHDRNLIIPIVLLVILVILMLLLRSIVAPL
                     LLVVTTVVSFATALGVAALLFNHVFSFPGADPAVPLYGFIFLVALGIDYNIFLVTRIRE
                     ETKTHGTRLGILRGLTVTGGVITSAGVVLAATFAALYVIPILFLAQIAFIVAFGVLIDT
                     LLVRAFLVPALFYDIGPKIWWPSKLSNQKYQKQPKL"
     gene            3914..4828
                     /gene="crtB"
     CDS             3914..4828
                     /codon_start=1
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /gene="crtB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: phytoene_synt"
                     /gene_kind="biosynthetic"
                     /product="phytoene synthase"
                     /protein_id="AAK64298.1"
                     /sec_met_domain="phytoene_synt (E-value: 6.6e-35, bitscore:
                     109.7, seeds: 8, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHQNSPLFLKSALRLYNRASSKASHKVIEEYSTSFSLSTWLLSP
                     RIRNDIRNLYAVVRIADEIVDGTAHAAGCSTTKIEEILDAYEIAVLAAPQQRFNTDLIL
                     QAYGETARRCDFDQEHVVAFFASMRKDLKANTHDPDSFTTYVYGSAEVIGLLCLSVFNQ
                     GRTISKKRLEIMQKGARSLGAAFQKINFLRDLADDQQNLGRFYFPDTNQGTLTKEQKED
                     LIADIRQDLAMAHEAFPEIPVQARIGVISAYLLFQKLTDQIEATPTSDLLRERIRVPLH
                     IKLSILASATMKGLSMSIYRKNS"
     gene            4825..6474
                     /gene="crtI"
     CDS             4825..6474
                     /codon_start=1
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /gene="crtI"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222:dehydrogenase (Score: 403.9; E-value: 2e-122)"
                     /gene_kind="biosynthetic-additional"
                     /product="phytoene desaturase"
                     /protein_id="AAK64299.1"
                     /sec_met_domain="DAO (E-value: 2.1e-10, bitscore: 29.7,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMKVSTKTPRSSGTAVVIGAGVAGLATSALLARDGWQVTVLEKNT
                     DVGGRAGSLEISDFPGFRWDTGPSWYLMPEAFDHFFALFGARTSDYLDLVELTPGYRVF
                     SGTHDAVDVPTGREKAIALFESIEPGAGAKLGNYLDSAEDAYDIAIDRFLYNNFSTLGP
                     LLHRDVLTRAGRLFSLLTRSLQKYVNSQFSSPVLRQILTYPAVFLSSRPTTTPSMYHLM
                     SHTDLVQGVKYPIGGFTAVVNALHQLALENGVEFQLDSEVISINTASSRGNTSATGVSF
                     LHNRKVQNLDADLVVSAGDLHHTENNLLPRELRTYPERYWSNRNPGIGAVLILLGVKGE
                     LPQLDHHNLFFSEDWTDDFAVVFDGPQLTRPHNASNSIYVSKPSTSEDGVAPAGYENLF
                     VLIPAKASSSIGHGDAYMQSASASVETIASHAINQIATQAGIPDLTDRIVVKRTIGPAD
                     FEHRYHSWVGSALGPAHTLRQSAFLRGRNSSRKVDNLFYSGATTVPGVGIPMCLISAEN
                     IIKRLHADTSAGPLPEPLPPKTTPSQKTSYDH"
     gene            6478..6927
                     /gene="crtYe"
     CDS             6478..6927
                     /codon_start=1
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /gene="crtYe"
                     /note="the gene products of crtYe and crtYf in combination
                     catalyze the formation of the cyclic C50 carotenoid
                     decaprenoxanthin from acyclic C50 carotenoids
                     (decaprenoxanthin synthases)"
                     /product="C50 carotenoid epsilon cyclase"
                     /protein_id="AAK64300.1"
                     /transl_table=11
                     /translation="MIPIIDISQNEQDSDIFMAFIYLGTLLVLIGCMALCDHRWKLAFF
                     RRPLRAIVSVGAAYIGFLLWDIFGIITGTFYRGDSAFMSGINLAPHMPIEELFFLFFLC
                     YITLNLTSAAALWLKAPLPEKPGRKPPLTPQRDTFQPTTSPEVKP"
     gene            6924..7316
                     /gene="crtYf"
     CDS             6924..7316
                     /codon_start=1
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /gene="crtYf"
                     /note="the gene products of crtYe and crtYf in combination
                     catalyze the formation of the cyclic C50 carotenoid
                     decaprenoxanthin from acyclic C50 carotenoids
                     (decaprenoxanthin synthases)"
                     /product="C50 carotenoid epsilon cyclase"
                     /protein_id="AAK64301.1"
                     /transl_table=11
                     /translation="MTYILISIPFLAIALVLFALKLQAGTPKLLPITAVSALTLCSLTI
                     IFDNLMVWADLFGYGDTQHLGIWLGLIPLEDLFYPLFAVLLIPALWLPGNMFKRRKKRP
                     HHSLPTIDNRSITTRSTTTQSAPEKP"
     gene            7319..8182
                     /gene="crtEb"
     CDS             7319..8182
                     /codon_start=1
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /gene="crtEb"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ubiA"
                     /gene_kind="biosynthetic-additional"
                     /note="a C5 prenyl unit transferring lycopene elongase,
                     adds to each end of the lycopene molecule one C5 prenyl
                     unit at position C-2 and C-2'; elongates lycopene (C40) to
                     C50 carotenoids"
                     /product="lycopene elongase"
                     /protein_id="AAK64302.1"
                     /sec_met_domain="ubiA (E-value: 1.4e-19, bitscore: 59.5,
                     seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMEKIRLILLSSRPISWVNTAYPFGLAYLLNAGEIDWLFWLGIVF
                     FLIPYNIAMYGINDVFDYESDIRNPRKGGVEGAVLPKSSHSTLLWASAISTIPFLVILF
                     IFGTWMSSLWLTISVLAVIAYSAPKLRFKERPFIDALTSSTHFTSPALIGATITGTSPS
                     AAMWIALGSFFLWGMASQILGAVQDVNADREANLSSIATVIGARGAIRLSVVLYLLAAV
                     LVTTLPNPAWIIGIAILTYVFDAARFWNITDASCEQANRSWKVFLWLNYFVGAVITILL
                     IAIHQI"
     CDS             complement(8191..9054)
                     /codon_start=1
                     /note="orf10"
                     /product="unknown"
                     /protein_id="AAK64303.1"
                     /transl_table=11
                     /translation="MTLRCSDVNVEPLPGTAKTGSGFVLLEHAGSWSRDVLDGGTFDPE
                     LTDQLKRHLKASGMGLQLIRKPGREGRNVEKHNLFLVFAEASIIEHLVVDAPADVLDLD
                     LSGPGKNNAQRMDDPMLLICTHSKRDVCCAIKGRPLAAAVEPQFGPLHVWEASHTKGHR
                     FAPSMLLMPWNYSYGLLDEAETVQLFQGALDNKLFLPGNRGRGTLDVRGQVAEIAVAEA
                     FDEAVAPASLQVEFEDDSVLVTHPDGRTWVVELERIEVDGVVSSCGDQPKTGKAWVARQ
                     VTELIG"
ORIGIN
        1 ccttacgcac aatatgtcca cctttttcta gcctattgag cgtctttgtt acagatgccg
       61 gggaaagttt aaggtgggct ccaagcattc ctggagtcac cacttcgccc gcattacccg
      121 ccacaatcaa atagtgcaaa gcccgcatat caggggcact tagctccata taacgtgccg
      181 aggcttcacc aatactacgt tccacatcgc gaagacttgc cagcgatttc attagccgcc
      241 caatttgcgc aatgtcttct tccgacatgc cagaacgatc aactaattca cttttaggat
      301 cactagtttt cacgtcataa atattgcgaa gtgtagattc tgccggatgg attttatctg
      361 gttcctgcat attcagcata ttaatcacct ttttttacaa agtcagataa agtttatttt
      421 caaaaatatt aaccgatgag atagtataaa tttcctgtag ggaccttaat ttttatgaac
      481 ccgatttcac accttctgta ctctttaaaa taaagcgcct tatagaacga ttgggaattt
      541 ttgcgacata actggccgtg cgatactcga atggacaatg gcatgacaat caccacagag
      601 cattcaactc atcctgatct tgatttcaat gatgagatct atcgggaact aaatcgcatc
      661 tgcgcttcgc tatctcaaca gtgcagcaca tatccaccag agttccgtac atgcctagat
      721 gccgctttcc aagctttgcg gggtggcaag ttaatccgtc ctcggatgct attagggcta
      781 tacgacgcgc ttgtagacga tgatattgag gtcaaactca acaccgtttt acaggtggca
      841 gcggctttag aactactaca tttttccctt ttggttcatg acgatgttat tgacggagac
      901 ctctatcgcc gaggcaaact taattttatt gggcagattc tcattcatcg cacacctgaa
      961 agttttgcac aaacccagcg cgatccagag catctagatt gggcacaatc taatgggcta
     1021 cttatgggga atctttttct tgctgccacc catcaaatct tcgcgcgcct tgaccttcca
     1081 cacccccaac gggttcggct gttagattta ctcaaccaca cgatgaatga caccattgtg
     1141 ggtgagtttc ttgatgtggg attaagcagc aaagcaatca gcccgaatat ggatattgct
     1201 ctagaaatga gtcggctaaa aacagccaca tacagttttg aacttccaat gagagcagcg
     1261 gcaattctcg cggaactacc tcaggagatt gaaacacaga taggtgagat aggcaccaac
     1321 ttgggcatcg cttaccaatt acaggatgat tacttatcta cttttggtga cgcagccgaa
     1381 cacggcaaag atgccttttc tgaccttcga gaaggaaaag aaacgacaat catcgcctat
     1441 gctcgaaata ctgctaaatg gaatgatatt gaagacatct tcggttccgc agatctgagc
     1501 acctctcagg cagagcgaat tcaacatctt ctcatacagt gtggagcaaa gaatcactcc
     1561 ttaaatgcca tctccgatca cttaaatatc tgccgttcga tgatcgaaac actaagcacc
     1621 caggtatatc ccaaggctca aaagttatta ctcaaacacg ttgagcaact agccagccgc
     1681 aaatcttaga actagctttt acgcctttaa cggaagtgaa tttgatgtct actagcatca
     1741 caacagagcg caggaagaaa tctggttcta ctcgcttgat gagaatcttt ctgcccgcct
     1801 tgctaatttt ggtttggctt gtaggagctg gaatcggcgg gccttatttt ggcaaggtta
     1861 gtgaggtctc ctccaacagc cagaccacgt atctgccaga atctgccgat gccactcagg
     1921 tacaggaaca gttgggagat ttcactgatt ctgaatccat cccagccatt gtcgtcatgg
     1981 tcagcgatga ccccttaacg caacaagata tcgcacaact caatgaagtt gttgctgggc
     2041 tctcagcatt agacatagtt tccgatgaag tctcccctgc tattccatct gaggacggca
     2101 gagctataca agttttcgtc cctctcagtc cgtctgcgga gctgacggaa agcgtcgaaa
     2161 agctttctga gaccctagcc cagcaaacgc cggactacgt gtctacctat ataaccgggc
     2221 ccgctggttt taccgccgat ctcagtgccg cttttgccgg tatcgacggg ttactcctcg
     2281 cagtcgcctt ggctgccgtg cttgtcatcc ttgtcatcgt ctatcgctca ttcattcttc
     2341 ccatcgctgt gcttgccacc agtttgtttg cgctgactgt agctctattg gtggtgtggt
     2401 ggctggccaa gtgggacata ctgctacttt cgggtcagac tcaaggcatc ctcttcattc
     2461 tggtcattgg tgctgccacc gactactcat tgctatacgt tgcacgtttc cgtgaagagt
     2521 tacgcgttca gcaagataaa gggatagcca cagggaaagc catccgggca tcggtggaac
     2581 ccattcttgc ctcgggcagc actgttattg caggcctcct ctgtttgcta tttagtgatt
     2641 tgaaatctaa ctccacgcta ggtccagtag cttcggtggg cattattttt gcaatgcttt
     2701 ctgctcttac tctgctacca gccctgctgt ttgtattcgg tcgggtggcc ttttggccca
     2761 agcgaccaaa atacgaacct gaagtagtcc atggtgaaaa tggcatcccc gccagcggga
     2821 tctggtcaaa agtggctcat ttggtggagc agcatcctcg tgcaatctgg gtatctacac
     2881 ttattgtgct tctcttgggt gcggctttcg ttcccacact aaaagcggat ggtgtgtccc
     2941 aatccgacct agttctgggt tcctctgaag cacgtgatgg ccagcaggct ttaggcgaac
     3001 acttccccgg tggatccggc agtcctgctt atattatcgt tgatgaaaca caggcagcac
     3061 aggttgctga cgtagtcctt aacaatgaca attttgaggc tgtaactgtc actagtgcag
     3121 actccccctc tggctcagcc cccatcaccg ctgacggtat tgtgccgtta gattctggca
     3181 cagctccagc cccggtagtt gtagaagggc aagtcctttt acaagcaaca cttgtcgaag
     3241 caccagattc cgaagaagct caaaaagcta ttcgcagtat ccgacaaact tttgcaaatg
     3301 aaaatatatc agcggtagta ggcggtgtca ctgcaacttc cgtagacact aacgatgcct
     3361 ccatccatga ccgcaacctg atcatcccaa ttgtattgct agtcattttg gttattctca
     3421 tgctgttgct gcggtctatt gtcgcaccac tcctgctagt agtcaccacc gtggtgtctt
     3481 ttgctactgc tttaggcgtg gctgctttac ttttcaatca cgttttcagt ttcccaggag
     3541 cagaccctgc agtgcctctc tacggattta tatttttggt agccttgggc atcgattaca
     3601 acattttctt agtcacccgt atccgtgaag aaaccaaaac ccacggcaca agacttggaa
     3661 ttcttcgagg tctgacagta accggcggag taattacctc agctggagta gttctcgccg
     3721 caacgttcgc agcactctat gtcatcccaa ttctattcct ggcacaaatt gccttcattg
     3781 tcgcttttgg agttcttatt gataccctgc tcgttcgcgc cttcttggtg cctgctttgt
     3841 tctacgacat cggaccgaaa atctggtggc cctcaaaatt gtccaatcag aaataccaga
     3901 agcagcctaa gctatgacac accaaaattc gcccctcttt cttaaaagtg cactgcgact
     3961 ttacaaccgg gcctcatcca aggcttcaca taaagtgatc gaagaatatt caacaagctt
     4021 cagtctgtct acgtggttgc tatccccacg catacgaaat gacatacgaa atctctatgc
     4081 ggtagtccgt atcgccgatg aaattgtcga cggcactgca catgccgctg gttgctcaac
     4141 taccaagatc gaagagattc tcgacgccta tgaaattgcg gttcttgcag caccacaaca
     4201 acgcttcaac acagatctca ttttgcaagc ttatggtgaa acagcccggc gttgtgactt
     4261 cgatcaggaa catgttgtgg ccttctttgc atcaatgcgt aaggacctca aagctaatac
     4321 acacgaccca gatagcttca caacgtatgt ctatggctcc gcggaagtta taggcctact
     4381 ttgtctcagc gttttcaacc aaggcagaac gattagcaaa aaacggctag agattatgca
     4441 aaaaggagcc cgctcattgg gagcggcatt ccagaaaatt aactttctcc gtgacttggc
     4501 agacgatcag caaaatttgg gccgttttta tttccccgat accaaccaag gaactcttac
     4561 taaagaacaa aaagaagatc tcatcgctga tattcgtcaa gacctggcaa tggctcacga
     4621 agcattccca gaaataccag tgcaggctcg catcggagtg atctctgctt atttgctctt
     4681 tcaaaaactc actgaccaaa ttgaggctac tcctacctcc gatttattgc gggagcgaat
     4741 cagggttcca cttcatatca aactctctat actcgctagc gccacgatga aaggtctatc
     4801 tatgagcatc tacagaaaga attcgtgatg aaggtatcaa ctaagactcc acgctcctca
     4861 ggtaccgccg tagtcatagg agcaggtgtt gccggtttag ccacctctgc acttttagct
     4921 cgtgatggct ggcaagtaac tgttttggaa aaaaacactg atgttggtgg ccgcgccgga
     4981 tcgctggaaa tatcagactt tcctggcttt cgatgggata ccggaccttc ttggtacctc
     5041 atgcccgagg cctttgacca tttcttcgca ctttttggtg cacgtacttc tgattatctc
     5101 gatttggtag aattaacgcc tggttatcga gttttttccg gcacacacga cgctgtcgat
     5161 gtccccactg ggcgtgaaaa agcaattgca ctattcgaat ccatcgaacc cggcgcgggt
     5221 gcaaagctag gaaattatct tgatagtgcg gaagacgcct acgacattgc cattgataga
     5281 ttcctttata ataatttctc cacgttaggc ccgctgcttc acagggatgt actgacccga
     5341 gctggccgac tgttttctct actgacccgt tctttacaaa agtacgtaaa tagtcagttc
     5401 agtagtccgg tattgcgcca gatcctgacc tatcctgcag tcttcctgtc ttcccggccc
     5461 actactaccc catcgatgta ccacttgatg agtcataccg atttggtgca gggagtgaaa
     5521 taccctatag gtggttttac tgcagtggtt aacgctctgc atcagttagc actggaaaac
     5581 ggggttgagt ttcaactcga ttctgaggtc atttccatca acactgcttc atctaggggc
     5641 aacacaagcg ccacaggtgt gagcttcctt cacaacagaa aagtgcaaaa tctagatgcg
     5701 gatcttgtgg tttcagcagg cgacctgcac cacacagaaa ataatctgct tccccgggaa
     5761 cttcgaacct atcccgaacg atattggtcc aatcgcaatc ctggaattgg agcggtatta
     5821 atcctcctgg gcgtaaaagg agagttaccc cagctcgacc atcacaatct tttcttcagt
     5881 gaagattgga cagatgattt tgctgtagtt ttcgacgggc ctcaacttac ccgcccccac
     5941 aatgcatcta attctattta tgtttccaag ccttcaacgt ccgaagacgg cgttgcacct
     6001 gctggatacg aaaacctttt tgttttaatt ccagccaagg cctctagcag catcggccac
     6061 ggtgatgcgt atatgcagtc ggcttcagca tccgtggaaa caatcgcgtc acatgcaatc
     6121 aatcaaattg ctacgcaagc cggcatccct gacctcactg accgaattgt ggtcaaacgc
     6181 actattggcc ctgcggattt tgaacaccgc taccattcat gggtaggcag tgcgctgggt
     6241 ccagcacata ctctcagaca gtctgctttc ttaagagggc gcaatagctc ccgcaaggtc
     6301 gataacctct tctattccgg tgccactacc gtcccaggtg taggaatacc catgtgttta
     6361 atttctgccg agaatattat taagcgttta catgccgata ccagtgcagg accactgccc
     6421 gaaccattgc cgcctaaaac gacaccatct caaaagacct catacgatca ttaaattttg
     6481 atccctatca tcgatatttc acaaaatgag caagatagcg atatttttat ggcctttatt
     6541 tatctaggta ctctcctagt tctcattggg tgcatggctt tgtgcgacca ccgttggaag
     6601 ctagcgttct tccgccgtcc cttacgagca attgtttcgg taggtgctgc atatattgga
     6661 tttcttttat gggatatatt tggcattatt actggcactt tttatcgcgg agactcagcg
     6721 tttatgtccg gtattaacct tgcgccccat atgcccattg aagaactttt tttcttattc
     6781 ttcctctgct acatcaccct caaccttacc tcggcagcag cattatggct taaagcacca
     6841 ctgcctgaaa aacccggtag aaagcctccc ctcacgccac agcgcgatac tttccaacca
     6901 actaccagtc ccgaggttaa accatgactt atattcttat aagcattcct tttttagcaa
     6961 tagccttggt cctatttgcc ttaaagctgc aggctggaac acctaaactt ttaccaatca
     7021 ccgctgtcag tgcccttacc ctatgttccc taactatcat atttgataac ctcatggttt
     7081 gggctgatct ctttggatat ggcgataccc agcatcttgg catttggctc ggtttaatcc
     7141 ccctagagga tcttttctat ccactcttcg cagtacttct gattcctgcc ctatggttgc
     7201 ctggaaatat gtttaaacgc aggaaaaaac gtccacacca ttccttaccc accatagaca
     7261 atcgaagcat cactactaga tccaccacca cacaatctgc cccagagaag ccgtagtcat
     7321 gatggaaaaa ataagactga ttctattgtc atctcgcccc attagctggg tcaataccgc
     7381 ctaccctttt gggctggcat acctattaaa tgcaggagag attgactggc tgttttggct
     7441 aggcatcgtg ttttttctta tcccgtataa catcgccatg tatggcatca acgatgtttt
     7501 tgattacgaa tctgacatac gtaatccccg caaaggcggc gtcgaggggg ccgtgctccc
     7561 gaaaagttcc cacagcacac tgttatgggc atcggctatc tcaacaattc ctttcctagt
     7621 tattcttttc atatttggca cctggatgtc gtctttatgg ctgacaatct cagtgctagc
     7681 agtgattgct tattcagcac cgaaattgcg ttttaaagaa cgccccttta tcgatgctct
     7741 aacatcttct actcacttca cttcacctgc attaatcggt gcaacgatca ctggaacatc
     7801 tccttcagca gcgatgtgga tagcactggg atcctttttc ttgtggggca tggccagtca
     7861 gatccttgga gcagtacagg atgttaatgc agaccgggaa gctaatctga gctcaattgc
     7921 cactgtaatt ggggcgcgtg gagccattcg gctatcagta gtactttatt tactagctgc
     7981 tgttttagtc actactttgc ctaatccggc gtggatcatc gggattgcga ttctaactta
     8041 cgtatttgat gccgcacgat tttggaacat tacagatgcc agttgtgaac aggctaatcg
     8101 cagttggaaa gttttcctgt ggctgaacta ctttgttggt gctgtgataa cgatactgtt
     8161 aatagcaatt catcagatat aactctgctt ttatccgatc agttctgtaa cttgcctagc
     8221 cacccacgct tttccagttt tcggctgatc accacacgag gacaccacgc cgtcaacctc
     8281 gatgcgttca agctccacaa cccacgtgcg cccatcggga tgagtaacca aaacagaatc
     8341 atcttcgaat tcaacctgca aactcgcagg agcaaccgcc tcgtcgaaag cttccgccac
     8401 ggcaatttct gcaacctggc cacgaacatc taaggttcct cggccacggt tgcccggcag
     8461 gaagagtttg ttgtccaacg cgccttggaa aagctgcacg gtttcggcct catcaagtag
     8521 gccataagag taattccacg gcatgagcag catcgatggc gcgaaccggt ggcccttggt
     8581 gtgcgaagcc tcccacacat gcagcggccc aaattgtggc tccacggcag ctgccagcgg
     8641 acgccccttg atcgcgcagc acacatcgcg cttcgagtgc gtacagatca gcaacatcgg
     8701 atcatccatg cgctgcgcat tatttttgcc cggcccgctt aaatcaagat ccaaaacatc
     8761 agccggcgcg tccaccacca ggtgctcaat aattgaggcc tcagcaaaaa cgagaaaaag
     8821 attatgcttt tcgacgtttc gaccctccct tcccggcttc ctaattaatt gcagacccat
     8881 tccggaagct ttcaggtgcc tcttcaattg atcagtcaac tcaggatcaa atgttccgcc
     8941 atctaaaaca tcacggctcc acgagccagc atgttcaagg agaacaaacc cagaacctgt
     9001 ttttgccgtt cccggcaggg gttcaacatt gacgtcagaa cagcgaagag tcatgtcctc
     9061 taccctagta tcgccgcctc catctgatgt ctggggtgtc tgtaattttc acgtctcctg
     9121 gcgtttttaa gacgaattac aacaattcgt tcacgattga gccaaattgg gcgtcttgct
     9181 taggtttcgg gggtgaccca gggttacggt gtgtgcatga ttgtgcagcc aac
//
