LOCUS       BGC0000646              6380 bp    DNA     linear   ENV 20-SEP-2009
DEFINITION  Uncultured bacterium clone RM3 carotenoid biosynthetic gene cluster,
            complete sequence.
ACCESSION   BGC0000646
VERSION     BGC0000646
KEYWORDS    .
SOURCE      uncultured bacterium
  ORGANISM  uncultured bacterium
            Bacteria; environmental samples.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 13:58:54
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6380
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..6380
                     /clone="RM3"
                     /db_xref="taxon:77133"
                     /environmental_sample=""
                     /isolation_source="soil"
                     /mol_type="genomic DNA"
                     /organism="uncultured bacterium"
     misc_feature    1..6380
                     /note="carotenoid biosynthetic gene cluster"
     protocluster    1..6380
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="True"
                     /core_location="[60:4727]"
                     /cutoff="20000"
                     /detection_rule="(Terpene_synth or Terpene_synth_C or
                     phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7
                     or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      61..4727
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(Terpene_synth or Terpene_synth_C or
                     phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7
                     or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
     cand_cluster    1..6380
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Terpene_synth or Terpene_synth_C or
                     phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7
                     or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..6380
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000646"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..6380
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="terpene"
                     /region_number="1"
                     /rules="(Terpene_synth or Terpene_synth_C or phytoene_synt
                     or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps
                     or fung_ggpps2 or trichodiene_synth or TRI5)"
                     /subregion_numbers="1"
                     /tool="antismash"
     CDS             61..1236
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: Lycopene_cycl"
                     /gene_kind="biosynthetic"
                     /note="lycopene cyclase"
                     /product="CrtY"
                     /protein_id="ACI04496.1"
                     /sec_met_domain="Lycopene_cycl (E-value: 3.1e-90, bitscore:
                     291.6, seeds: 20, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNPAVPPSAPTGLDLVLVGGGLQSALIALSVLERRPDASLVMLEG
                     SDRIGGNHTWSFHAGDVPEAAHGFVEPLVEHRWPAYDVAFPGHERRLDQGYATISSDRL
                     AAVVTERLRAAPHAVLHTGARAVEVTGTSVTLDDGRQFAGKAVIDSRGPGRLPPAPGTG
                     FQKFVGLELDLAEPSPRTVPLLMDACVQQTDGFRFFYVLPLAERRVLIEDTYFSDTADL
                     GLAAISREILAYARARGFVVSGVVRRESGALPLPVRALPVLHSVSPLVAGYAGGWFHPA
                     TGYSLPAAVRLALHVGQTPAEDLFGADWERLVARHRQQFRFGALLNKMLFTAFAPEDRW
                     NALARFYRMPEATIARFYSLETTQADRLRVLCGRPPRGLSARTAISRGAFA"
     CDS             1233..2288
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182:Polyprenyl synthetase (Score: 131.9; E-value:
                     4.1e-40)"
                     /gene_kind="biosynthetic-additional"
                     /note="octaprenyl-diphosphate synthase"
                     /product="CrtE"
                     /protein_id="ACI04497.1"
                     /transl_table=11
                     /translation="MTTTARVLESPSPLVDALERELHPDPSSGLLGGALREVPWHVWHR
                     ALGAPLHEFLQRPGKEFRAELTRIFFALGGRKEPPPENLALIVEVLHAGSLIVDDIQDE
                     SEERRGGPALHQIHGVPLALNAGNWLYFWAYALLGEIDLPDEAAVRAHRLVTRALLDCH
                     AGQGLDLSVRMSDVAQAAVPAVVRASTLLKTGSLLGLAAGLGALVGGATADAIAASARF
                     GQDLGTGLQMLDDLSGLVSPRRAHKLRGPRQRPTLLGVGLARGESGAGGVPGAPRGSAA
                     RRAEARLVAAGGAPGQLMSQMRALLMPDARRIVHDHLESAFGRLTAAVPGPLSTTTALR
                     RELDRLEKSYV"
     CDS             2281..3801
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222:dehydrogenase (Score: 434.5; E-value: 1.1e-131)"
                     /gene_kind="biosynthetic-additional"
                     /note="phytoene desaturase"
                     /product="CrtI"
                     /protein_id="ACI04498.1"
                     /transl_table=11
                     /translation="MSEPLSSQATRVAIVGAGFGGLAAAIRLQAAGMQTVLFESRDKPG
                     GRAYVYEDEGFVFDAGPTVITAPEAFEEVFAAAGRRLSDYVEFIPVAPYYRLIWDDGDT
                     FDYGPDADAMLEEIRRRNPADARGYLDFVEHSRKVFEKGYTDLAAVPFQRFMDMVKVAP
                     DLARLRADRSVYATVARFVKDDHVRQALSFHSLLVGGNPFDTSSIYTLIHYLERKWGVF
                     FPRGGTGALVAAMVRLYEELGGELRLNAPVDRIQVQDQRGRVTHRVESGGQTEVFDVVV
                     SNADVHHTYSRLYAGHPVAARRSRKLERLDWSMSLFVLHFGVNRVYPGIAHHTVLFGPR
                     YRELLREIFDGPDLSADFSLYLHSPTVTDASLSPPGCSTFYVLSPVPHLGKANIDWSAA
                     AGEYAERILTSLERLLPDLRQHVVTKRWFTPADFQGELGAFQGSAFSVAPTLTQSAWFR
                     PHNRDDDIPGLYLVGAGTHPGAGLPGVVSSAKATASLILADFHAETAA"
     CDS             3798..4727
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: phytoene_synt"
                     /gene_kind="biosynthetic"
                     /note="phytoene synthase"
                     /product="CrtB"
                     /protein_id="ACI04499.1"
                     /sec_met_domain="phytoene_synt (E-value: 1.7e-63, bitscore:
                     203.0, seeds: 8, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAALTVCRASIAQHSKSFSLASRLLGPRERDAAAAVYAFCRRA
                     DDAVDIASDRSPVEIVATLRAELDAIYAGEVQSDPALVAFQEVVRAHHIPKDYPAELLA
                     GMAMDVEDTTYDDLDALLVYCFRVAGTVGLMMSHVLGVREEAALHNAAHLGMAMQLTNI
                     CRDVGEDWGRGRLYLPSADLDRAGAPGLRGRLGGPLPDAARPPIARVIRALLERAELLY
                     ESGERGVPLLPFRAAVAVRTARLVYGDIGRVILEQGADPVAPRAVVSTSRKLRLALLAF
                     ARTAAQVPLEFRGAELFHVVGVHDVIPS"
     CDS             4711..5298
                     /codon_start=1
                     /note="similar to Bdellovibrio bacteriovorus HD100 protein
                     Bd1723"
                     /product="hypothetical protein"
                     /protein_id="ACI04500.1"
                     /transl_table=11
                     /translation="MSFPAEVAVYVGRVRQFDRADWVVYVAWVGLMLGLCGSTGGFLLF
                     GDAHGVTFPVAAWLVPVGAMLFTASIAIDTIGHRTIYKEVIAGAEGLVHGITILCGIGS
                     TVLLCAAYGRPGAMWIPAMVLTVLSFVYSLVDEAFHWRRYVMQHADRVEMWSHLGILVG
                     HGTMMVAWWVWFFGGYSGVAETLAHLSMGSAR"
     CDS             5295..6380
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1062:glycosyltransferase, MGT family (Score: 119.9;
                     E-value: 2.6e-36)"
                     /gene_kind="biosynthetic-additional"
                     /note="putative glycosyltransferase"
                     /product="CrtX"
                     /protein_id="ACI04501.1"
                     /transl_table=11
                     /translation="MKKKILLFSEGVTLSQVVRLVTLGRSLDPARYDVTFACSEFDELA
                     FGGTSFRRRLVHSLDPKAVHRALRQGRRLYDRKTLLAQVEEDLAVFDEVRPDVVVGDFR
                     WSLAVSAPVHGVPHAALINAYWSPYATTRTFPVPDHPIVRLLGEAMTAKYFPVALPRVF
                     EHFAAPLNEVRKVHGLPPVGSLLEVLTHGDHVLYADPPELVPTTSAPANHEHLGYVPWA
                     PGGPLPGFWNDLPADRPVVYATVGSSGDVDTLPLVVEALGGLPVTALVATAGRKTLPRL
                     PANVYAADFLPGDLAARRSALVISNGGSSTSYQALAEGVPVLGLASNLDQYLAMAAIEA
                     SGAGQLARARLATAPSLRATR"
ORIGIN
        1 gtcggtcgtg gcgtctgagg cttgacagcg cgtagttcat tacctattca aaacctggtc
       61 atgaatcccg ccgtcccccc ctcagccccg accggactgg acctggtcct cgtcgggggc
      121 gggcttcaga gcgccctgat cgccctgtcc gtgctggaac ggcggccaga cgcctcgctg
      181 gtgatgctgg aggggtcgga ccggattggc gggaaccaca cctggtcgtt ccatgcgggc
      241 gacgtgcccg aggcggcaca cgggttcgtc gagccgctcg tcgagcaccg gtggccggcc
      301 tacgacgtgg cgttccccgg ccacgagcgg cggctggacc agggctacgc cacgatctcg
      361 agcgaccgcc tggccgccgt ggtgaccgag cggctccgcg ccgcgccaca cgccgtgctc
      421 cacaccgggg cgcgcgcggt cgaggtcacc gggacctccg tgaccctcga cgacgggcga
      481 cagttcgccg gcaaggcggt catcgactca cgcgggcccg gccggttgcc gcccgcgccc
      541 ggtactggct ttcagaaatt cgtcgggctc gagctcgacc tcgcggagcc gagcccgcgg
      601 acggtgccgc tcctgatgga tgcgtgtgtc cagcagacgg acggttttcg cttcttctac
      661 gtgctgccgc tcgcggagcg gcgcgtgctg atcgaagaca cgtacttctc ggacaccgcg
      721 gacctcggcc tggcggcgat cagccgcgag atcctggcct acgcgagggc tcgcgggttc
      781 gtcgtgagcg gcgtggtccg gcgggaatcc ggcgccctgc cgctgccagt gcgcgcattg
      841 cccgtcctgc attccgtgtc gccgctcgtg gcgggctacg ccggcggctg gtttcacccc
      901 gccaccggct actcgctgcc ggccgcggtg cggctggccc tccacgtggg gcagacgccg
      961 gcggaggacc tgttcggcgc cgactgggag cggctcgtcg ctcgtcaccg ccagcagttt
     1021 cgcttcggcg cgctgctcaa caagatgctg ttcaccgcct tcgccccgga ggaccggtgg
     1081 aacgcgctcg cgcggttcta ccggatgccg gaggccacga tcgcccgatt ctactcgctc
     1141 gaaaccacgc aggccgatcg cctgcgcgtg ctctgcgggc gtcccccgcg cggtctctcg
     1201 gcaagaacgg ccatctctcg aggagctttc gcatgaccac cacagcccgg gttctggagt
     1261 caccctctcc cctcgtcgac gcgctcgagc gcgagctgca ccccgacccc agctcggggc
     1321 tgctcggtgg cgcgctccgt gaggtgccgt ggcacgtctg gcaccgcgcg ctcggcgcgc
     1381 ccctgcacga gttcctccag cgcccgggca aggagttccg tgccgagctc acgcgcatct
     1441 tcttcgccct gggtggaagg aaggaacctc ctccggagaa tctggcgctg atcgtggagg
     1501 tgctccacgc ggggtcgctc atcgtggacg acatccagga cgagtcggag gagcgacgcg
     1561 gcgggccggc gctccaccag atccacggcg tgccgctcgc cctgaacgcc ggcaactggc
     1621 tctacttctg ggcctacgcg ctcctgggcg agatcgacct gcccgacgag gcggcggtgc
     1681 gcgcccatcg actggtgacg cgcgcgctcc tcgactgcca cgccgggcag gggctcgacc
     1741 tgtcggtgcg gatgagcgac gtcgcgcagg cggcggttcc cgcggtggtc cgcgcctcca
     1801 cgctgctcaa gacaggcagc ctgctcgggc tcgccgccgg gctcggagcc ctcgtcggcg
     1861 gagcgaccgc ggacgcgatc gcagcgtccg cgcgcttcgg ccaggacctg ggcaccggcc
     1921 tgcagatgct ggacgatctg agcggcctcg tgagcccccg gcgggcccac aagctccgag
     1981 gacctcgtca acgaccgacc ctcctgggtg tgggcctggc tcgcggtgag tcgggcgccg
     2041 gtggagtacc aggcgctccg cgcggaagcg cggctcgtcg cgcggaagcg cggctcgtcg
     2101 ccgccggcgg agccccgggg cagctcatga gccagatgcg cgcgctgctc atgcccgacg
     2161 ctcgacggat cgtgcacgac cacctggaga gtgcgttcgg gcgactcacc gcagccgtcc
     2221 ccgggccgct ctcgaccacg acggcgctcc gccgcgagct cgatcgcctg gagaagagct
     2281 atgtctgagc cgctgtcctc ccaggccacg cgcgtggcga tcgtcggcgc cgggttcggc
     2341 ggcctcgcgg ccgccatccg cctccaggcg gcggggatgc agacggtgct cttcgagtcg
     2401 cgtgacaagc cgggtggccg tgcatacgtg tacgaagatg agggtttcgt gttcgacgcg
     2461 ggacccacgg tcatcaccgc gcccgaggcg ttcgaggagg tcttcgccgc cgccgggcgc
     2521 cgcctgtccg actacgtgga gttcatcccg gtggcgccct actaccgcct catctgggac
     2581 gacggcgaca ccttcgacta cgggcccgac gcggacgcga tgctggagga gatccggagg
     2641 cggaaccccg ccgacgcgcg cggatacctg gatttcgtcg agcactcgag gaaggtgttc
     2701 gagaagggct acacggacct ggccgcggtc cccttccagc gcttcatgga catggtgaag
     2761 gtggccccgg atctcgcccg cctgcgggcg gaccggtcgg tgtacgccac ggtcgcgcgg
     2821 ttcgtgaagg acgaccacgt gcggcaggcg ctcagcttcc actcgctgct cgttggcggc
     2881 aacccgttcg acacgagctc gatctacacg ctcatccact acctggagcg aaagtggggc
     2941 gtgttcttcc cgcgcggcgg caccggagcg ctcgtcgctg cgatggtccg gctctacgag
     3001 gagctcggcg gcgagctccg cctgaacgcc ccggtggatc gcatccaggt gcaggatcag
     3061 cgcgggaggg tcacccaccg cgtcgagagc gggggacaga ccgaggtctt cgacgtggtc
     3121 gtctcgaacg ccgatgtgca ccacacctac tcgcgcctgt acgcggggca cccggtcgcc
     3181 gcgcgtcgct cgcggaagct cgagcgcctg gactggtcga tgtccctctt cgtgctgcat
     3241 ttcggcgtga accgcgtgta ccccggcatc gcgcatcaca cggtgctgtt tggcccgcgc
     3301 taccgggagc tcctccggga gatcttcgac ggccccgatc tgtccgcgga tttcagcctc
     3361 tacctgcact cgcccaccgt caccgacgcg tccctgtcgc ccccgggctg ctccaccttc
     3421 tacgtgctga gcccggtccc gcacctcggc aaggcgaaca tcgactggtc ggcggccgcg
     3481 ggcgagtacg cggagcggat cctcacctcg ctcgagcggc tgctgccgga cctccggcag
     3541 cacgtggtca cgaagcgctg gttcacgccg gccgacttcc agggggagct gggcgcgttc
     3601 cagggctcgg cgttctcggt ggcgcccacg ctcacccaga gcgcctggtt ccgtccgcac
     3661 aaccgtgacg acgacatccc gggtctctac ctggtcggcg ccgggacgca ccccggggcc
     3721 gggctgcccg gcgtcgtgag ctcggccaag gctacggcgt cgctgatcct ggcggatttc
     3781 cacgcggaga cggcagcatg acgacggccg ccctcaccgt gtgtcgcgcg tcgatcgcgc
     3841 agcacagcaa gagcttctcg ctggcgtccc ggctgctcgg cccccgcgag cgcgacgcgg
     3901 cggcagccgt ctacgcgttc tgtcgccggg cggacgacgc cgtggacatc gcgtcggatc
     3961 gctccccggt cgagatcgtc gccacgctgc gcgcggagct cgacgcgatc tatgccggag
     4021 aggtgcagtc ggaccccgcc ctcgtggcgt tccaggaggt cgttcgcgcc caccacatcc
     4081 cgaaggacta ccccgccgag ctcctcgccg gcatggcgat ggacgtggag gacaccacct
     4141 acgacgatct cgacgcactg ctcgtgtact gcttccgcgt ggccgggacg gtcgggctga
     4201 tgatgagcca tgtgctcggg gttcgggagg aagcggcgct ccacaacgcg gctcacctcg
     4261 ggatggcgat gcagctcacc aacatctgcc gggacgtcgg cgaggactgg ggccggggca
     4321 ggctctacct gccgtccgcc gatctggacc gcgcaggcgc cccggggctc cgcgggcgac
     4381 tgggtggacc gctcccggac gctgcccgtc cgccgatcgc gcgggtcatc cgggctctgc
     4441 tcgaacgcgc cgagctgctc tacgagtcgg gcgagcgagg ggtgccgctt ctgcctttcc
     4501 gcgcggcggt ggcggtccgc accgcgcggc tggtctacgg ggacatcggt cgggtgatcc
     4561 tggagcaggg agccgacccc gtcgcgccac gcgctgtcgt ctccacgtcg cggaagctgc
     4621 ggctggcgct cctggccttc gctcgcaccg cggcgcaggt gccgctcgaa tttcgaggtg
     4681 cggagctctt ccacgtcgtc ggggtccacg atgtcattcc cagctgaggt cgcggtctac
     4741 gtcggcaggg ttcgccagtt cgaccgggcg gactgggtgg tctacgtggc gtgggtcggg
     4801 ctcatgctcg gcctgtgtgg ctccaccgga gggttcctgc tcttcgggga cgcccacggg
     4861 gtgaccttcc ccgtggcggc gtggctcgtc ccggtggggg cgatgctctt caccgccagc
     4921 atcgccatcg acacgatagg tcaccggacc atctacaagg aggtcatcgc cggcgccgag
     4981 gggctcgtgc acggcatcac catcctctgc gggatcggca gcacggtgct cctgtgcgcc
     5041 gcctacggtc ggcccggcgc catgtggatc ccggcgatgg tgctgacggt gctgtcgttc
     5101 gtctacagcc tcgtggacga ggcctttcac tggcgcaggt acgtgatgca gcacgccgac
     5161 cgcgtggaga tgtggagcca cctcggcatc ctcgtcggcc acggcaccat gatggtggcg
     5221 tggtgggtgt ggttctttgg cgggtactcg ggcgttgcgg agacgctcgc gcacctgtcg
     5281 atggggagcg cgcgatgaag aagaagatcc tgctgttctc ggagggcgtg acgctgtcac
     5341 aggtggtccg gctggtcacg ctggggaggt ccctcgatcc tgccaggtac gacgtcacct
     5401 tcgcctgctc cgagttcgac gagctcgcgt tcggcgggac gtcgttccgg cggcgtctcg
     5461 tccactcgct cgatccgaag gcagtgcacc gcgcgctccg tcaggggcgg cggctgtacg
     5521 acaggaagac gctcctcgcg caggtggagg aggatctggc cgtgttcgac gaggtgcgcc
     5581 ccgacgtggt ggtcggcgat ttccgctggt cgctcgccgt gagcgcgccc gtgcacgggg
     5641 tcccgcacgc ggcgctcatc aacgcctact ggagcccgta cgcgacgacc cgcacgttcc
     5701 ccgtgccgga ccaccccatc gtgcggctcc ttggcgaggc catgaccgcg aagtacttcc
     5761 cggtcgccct gccgcgcgtg ttcgagcact tcgcagcgcc cctcaacgag gtccggaagg
     5821 tgcacgggct gccgcccgtg ggtagcctgc tcgaggtgct gacgcacggc gaccacgtgc
     5881 tctacgccga tccgccggag ctcgtcccga cgacctcggc cccggcgaac cacgagcacc
     5941 tgggctacgt cccctgggcg cccggagggc cgctgcccgg gttctggaac gacctgccgg
     6001 ccgatcgccc cgtggtctac gcgacggtcg gctcgtcggg cgacgtcgac acgctgccgc
     6061 tggtcgtgga ggccctggga ggcctgcccg tcaccgcgct cgtggcgacc gcggggcgga
     6121 agacgctgcc ccgcctgccg gccaacgtgt acgccgcgga cttcctgccg ggcgatctcg
     6181 cggcgcggcg ctcggccctc gtgatctcga acggcggcag cagcacctcg taccaggcgc
     6241 tggcggaggg cgtcccggtg ctggggctgg cctccaacct cgatcagtac ctggcgatgg
     6301 cggcgatcga ggcctccggg gccggtcagc tggcccgcgc gcgcctcgcc acggcgccga
     6361 gcctgcgggc gacgcgctga
//
