LOCUS       BGC0000649             10878 bp    DNA     linear   BCT 16-AUG-2001
DEFINITION  Streptomyces griseus strain IFO13350 carotinoid biosynthesis gene
            cluster, complete sequence.
ACCESSION   BGC0000649
VERSION     BGC0000649
KEYWORDS    .
SOURCE      Streptomyces griseus subsp. griseus NBRC 13350
  ORGANISM  Streptomyces griseus subsp. griseus NBRC 13350
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 13:59:10
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..10878
                     /db_xref="taxon:455632"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces griseus subsp. griseus NBRC 13350"
                     /strain="IFO 13350"
                     /sub_species="griseus"
     protocluster    1..10878
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="True"
                     /core_location="[5136:10747]"
                     /cutoff="20000"
                     /detection_rule="(Terpene_synth or Terpene_synth_C or
                     phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7
                     or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5137..10747
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(Terpene_synth or Terpene_synth_C or
                     phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7
                     or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
     cand_cluster    1..10878
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Terpene_synth or Terpene_synth_C or
                     phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7
                     or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..10878
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000649"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..10878
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="terpene"
                     /region_number="1"
                     /rules="(Terpene_synth or Terpene_synth_C or phytoene_synt
                     or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps
                     or fung_ggpps2 or trichodiene_synth or TRI5)"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            149..1771
                     /gene="fadD"
     CDS             149..1771
                     /NRPS_PKS="Domain: AMP-binding (8-438). E-value: 6.7e-67.
                     Score: 217.7. Matches aSDomain:
                     nrpspksdomains_fadD_AMP-binding.1"
                     /codon_start=1
                     /gene="fadD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002:AMP-dependent synthetase and ligase (Score:
                     384.3; E-value: 1.2e-116)"
                     /gene_kind="biosynthetic-additional"
                     /note="putative long chain fatty acid synthetase"
                     /product="FadD"
                     /protein_id="AAG28698.1"
                     /sec_met_domain="AMP-binding (E-value: 3.9e-73, bitscore:
                     235.4, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEYNLADLFESVVDAVPDREALLYVDHPGTGAERRLTYAELDAAA
                     NRIAHHLIDAGIRPGEHVGLHLYNGIEYLQTVLGALKARIVPVNVNYRYVEEELVYLYR
                     DADLAALVFDGEFDERVAAAAPRCPGLRHLVRVGAAPASPPGVPVVPFAEAEAAGAAGR
                     GFGPRSADDQFIIYTGGTTGMPKGVMWRQEDLFFSGLGGGAPTGDPVQAPRELAERVAA
                     GGDGITFFPTPPLMHGTSTLTAFIGFNFGQRVVVHRKFVPAEVLRTIEREKVTSVSLVG
                     DAMLRPLIDCLKGPLRGTDCSSLFSVSSSGAIMSDSVRAEFQELVPTVLLLNNFGSSES
                     GFNGTATADSGPERGFRVQVNARTAVVDPVTYEPVAPGEPGRIAQRGHVPLGYYNDPAK
                     TADTFFRRGEERWVLLGDMATVDADGIVTVLGRGSQCINTGGEKVYPEEVEQALKSHPD
                     VYDALVAGVPDERWGNRVAAVVQLREHARGLTLDEVQAHCRTRLAGYKIPRALVLADRI
                     QRSPSGKADYRWAKAVAAGAGRA"
     aSDomain        173..1462
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_fadD_AMP-binding.1"
                     /evalue="6.70E-67"
                     /label="fadD_AMP-binding.1"
                     /locus_tag="fadD"
                     /protein_end="438"
                     /protein_start="8"
                     /score="217.7"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="FESVVDAVPDREALLYVDHPGTGAERRLTYAELDAAANRIAHHLI
                     DAGIRPGEHVGLHLYNGIEYLQTVLGALKARIVPVNVNYRYVEEELVYLYRDADLAALV
                     FDGEFDERVAAAAPRCPGLRHLVRVGAAPASPPGVPVVPFAEAEAAGAAGRGFGPRSAD
                     DQFIIYTGGTTGMPKGVMWRQEDLFFSGLGGGAPTGDPVQAPRELAERVAAGGDGITFF
                     PTPPLMHGTSTLTAFIGFNFGQRVVVHRKFVPAEVLRTIEREKVTSVSLVGDAMLRPLI
                     DCLKGPLRGTDCSSLFSVSSSGAIMSDSVRAEFQELVPTVLLLNNFGSSESGFNGTATA
                     DSGPERGFRVQVNARTAVVDPVTYEPVAPGEPGRIAQRGHVPLGYYNDPAKTADTFFRR
                     GEERWVLLGDMATVDADGIVTVLGRGSQCIN"
     CDS_motif       668..718
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_fadD_0001"
                     /evalue="3.00E-06"
                     /label="NRPS-A_a3"
                     /locus_tag="fadD"
                     /protein_end="190"
                     /protein_start="173"
                     /score="19.8"
                     /tool="antismash"
                     /translation="FIIYTGGTTGMPKGVMW"
     misc_feature    1034..1036
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     CDS_motif       1313..1360
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_fadD_0002"
                     /evalue="3.70E-04"
                     /label="NRPS-A_a6"
                     /locus_tag="fadD"
                     /protein_end="404"
                     /protein_start="388"
                     /score="13.0"
                     /tool="antismash"
                     /translation="VPLGYYNDPAKTADTF"
     gene            2343..3620
                     /gene="crtE"
     CDS             2343..3620
                     /codon_start=1
                     /gene="crtE"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182:Polyprenyl synthetase (Score: 262.3; E-value:
                     8.3e-80)"
                     /gene_kind="biosynthetic-additional"
                     /note="GGPP synthetase"
                     /product="CrtE"
                     /protein_id="AAG28699.1"
                     /transl_table=11
                     /translation="MGRRQPEEPSFGESARPAPGTGGHRHHAAPSSEDARVVDADVVDA
                     VLRTARAVLAERVDEAMASDALFCDELARRVADFTLDGGKRVRPRLLWWGMRSCGAADT
                     TAALRLGVALELIQSCALIHDDVMDRSRLRRGKPAVHIGLAERAGYSPDSGRGSAFGAS
                     AAVLAGDLALAWADDTVAETALSAVQRRRIGPLWRTMRTEMVAGQYLDLRGQVRGGSSV
                     EQALRTACLKSASYSVERPLALGAALAGADEATTDALRSAGRCAGIAFQLRDDLLGVFG
                     DPELTGKPSGDDIREGKSTYLLPVARRWAEPAGNKQSLAVLGRFPGGGNLAKEDLPEVR
                     GVLEETGARAHVERKADPLRDHALGRLAQAVEVEAHGGRQLLGLLRTVSGERTGQSPAT
                     GGTHGHGPGAAAAAPALTTAEGGTTR"
     gene            3617..5140
                     /gene="crtI"
     CDS             3617..5140
                     /codon_start=1
                     /gene="crtI"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222:dehydrogenase (Score: 501.6; E-value: 4.8e-152)"
                     /gene_kind="biosynthetic-additional"
                     /note="phytoene synthetase"
                     /product="CrtI"
                     /protein_id="AAG28700.1"
                     /sec_met_domain="DAO (E-value: 6.4e-21, bitscore: 64.3,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MITVKGRVDHVVVVGAGLAGLSAALHLLGAGRRVTVVERDSLPGG
                     RAGLLESGGFRIHTGPTVLTMPDLVEEAFAAVGEATADRLELIRPAPAYRARFADGSRL
                     DVHTDGAAMEAAVEEFAGARHAAGYRRLMIWLERLYRAQMRRFIDTNFDSPFQLVHPDL
                     ARLAALGGFGRLDARIGHFVSDERLRRVFSFQALYAGVPPARALAAYAVIAYMDTVAGV
                     YFPRGGMHALPRAMADAAADAGASFRYGQSVTRLERSGDRVTAVVTDQERIPCDAVVLT
                     PDLPVSYRLLGRSPRRPLPLRHAPSAVILHAGTDRTWPELAHHTISFGAAWKGTFHELT
                     RAGKPMSDPSLLITRPTASDPSLAPPGKHLHYVLAPCPNTEVGPGVREWRELGPRYRDQ
                     LVAELERRDMPGLGAAIEEEVLVTPVDWTAQGHAAGTPFSVAHTFPQTGPFRPRNLVRG
                     TANAVLAGCGTTPGVGVPTVLISGKLAAERITGPRATRTPRPTPGGSPA"
     gene            5137..6165
                     /gene="crtB"
     CDS             5137..6165
                     /codon_start=1
                     /gene="crtB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: phytoene_synt"
                     /gene_kind="biosynthetic"
                     /note="phytoene dehydrogenase"
                     /product="CrtB"
                     /protein_id="AAG28701.1"
                     /sec_met_domain="phytoene_synt (E-value: 7.5e-108,
                     bitscore: 348.9, seeds: 8, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTVRELDAAGIHDPALRAAYTRCRVLNARHGRTYFLATRLLPVDR
                     RPAVHALYGFARRADDIVDDLDSSATPGERARALLDLEARLEAGLRGAPAAEPVIRALA
                     DTAGRYAIDHRHFTDFLRSMRSDLTVGGYASYEELGRYMHGSAAVIGLQMLPVLGTVVP
                     REESAPHAAALGVAFQWINFLRDVGEDVDPERLYLATELLGAHRADRDRFESSRSTGMP
                     DSRITDVLPAAAAHYRAVYRAALPGIAMLAPVSRPCIRTAFVLYSGILDAIEADGYALL
                     HRPAVVPRRRPAAVPLDDLVPAVAVRTLCRRPAHAVDASRTAELSGPRTAGRPARVRTE
                     AV"
     gene            6162..7178
                     /gene="crtV"
     CDS             6162..7178
                     /codon_start=1
                     /gene="crtV"
                     /note="putative methyltransferase-like enzyme"
                     /product="CrtV"
                     /protein_id="AAG28702.1"
                     /transl_table=11
                     /translation="MRPAGRRRRTGRHPLPVRRAPDPWQRPARTRRDAQPSLTTDALER
                     APSTPSGNWYVIGASRALPPGKALGVTVGSTEVVAWRDGAGKLRTGPGACPHLGAPLRD
                     SPVRCGTLVCHWHGLALDGGPFAGWEPYPAFDDGVLLWVRLDREGGEPPSQRPRVPSRP
                     RVRDAVEAVYCGVGRCEPEDVVANRLDPWHGAWFHPYSFVDLTVTGTPEDGPADGDGFT
                     VDVSFKVAGRLVVPVRARFTAPGPRTVVMHIVEGEGAGSVVETHATPLAPDRWGKPRTA
                     VTEALVAASDRRGFAVARAAAPLLRPLMRASAGRLWRDDLAYAERRWELRDRGTFPG"
     gene            complement(7228..8781)
                     /gene="crtU"
     CDS             complement(7228..8781)
                     /codon_start=1
                     /gene="crtU"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_kind="biosynthetic-additional"
                     /note="beta-carotene desaturase"
                     /product="CrtU"
                     /protein_id="AAG28703.1"
                     /sec_met_domain="DAO (E-value: 1.4e-13, bitscore: 40.2,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLRRDGDGGHRHGRDRRAEVVPAPAGRARFTGDAPAVAVVGGGIA
                     GIAAATLLAERGARVTLHESEEGLGGRLAGWPTRLADGTTVTMSRGFHAFFRQYYNLRG
                     LLRRVDPGLGSLTRLPDYPLRHSSGLRDGFARVPRTPPLSALGFVALSPTFGPGDLVRM
                     NPRAALGLLDVRVPEVYERLDGISATDFLHRIRFPEAAHHLAFEVFSRSFFADPRELSA
                     AELALMFHIYFLGSAEGLIFDVPRETFPAALWDPLHRYLEGHGVDVRTRTPVREVRPLP
                     EGGAVLTTDDLTTRYDALVLALDSGGLRHLVAASPELGDADWRDRIARLRTAPPFLVSR
                     LWLDRPVAHDRPGFLGTSGYGPLDNVSVLDRWEGEAARWARRTRGSVVELHAYAVAPDA
                     DRAAVQAETLGQLHRVYPETRSARLLDERHEWRADCPMFPVGGYRDRPGVRSPDPTVTV
                     AGDMVRTGLPVALMERAATSGFLAANALLERWGVSGQTLWTVPRAGRSAVLRRLAAVAD
                     "
     gene            complement(8781..9509)
                     /gene="crtT"
     CDS             complement(8781..9509)
                     /codon_start=1
                     /gene="crtT"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089:methyltransferase (Score: 80.9; E-value:
                     1.7e-24)"
                     /gene_kind="biosynthetic-additional"
                     /note="putative methyltrasferase-like enzyme"
                     /product="CrtT"
                     /protein_id="AAG28704.1"
                     /transl_table=11
                     /translation="MTLLRGDALSEAFDHGSAAYDRLVAASPGYHAHLRRSARRLGLPE
                     GGRGLRVLDLGCGTGASTAALLAAAPLARITGVDASAGMLARATAKSWPQGVDFAHHPV
                     EELAGRLEAGSFDAVFAGYLFRNLADPDQALDLVRGLLVPGGRLAVHEYTLGGSPAHLA
                     AWRTICSLIIQPAGRLAGDTGLYRHLRDSVTRFDTAPAFARRLRRAGFTDVRVLPLPGW
                     QTGITHTFVARTAAGPGGRA"
     gene            complement(9506..10747)
                     /gene="crtY"
     CDS             complement(9506..10747)
                     /codon_start=1
                     /gene="crtY"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: Lycopene_cycl"
                     /gene_kind="biosynthetic"
                     /note="lycopene cyclase"
                     /product="CrtY"
                     /protein_id="AAG28705.1"
                     /sec_met_domain="Lycopene_cycl (E-value: 9.6e-108,
                     bitscore: 349.6, seeds: 20, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPADFDVVIVGAGAAGMSLAYHLCAPGADVPVSVALVDAPPGPLR
                     APPRTWCFWEPPGGPYDQSLAASWPRLRIRAADGAATVARLSRLRYKMLCSDAFEALVE
                     RRLARAPRLCRMEATASAVRDDPSGARGEVLTRGPGGERVLVTGRLVFDSRPPRRLPPA
                     RTTLLQHFTGWFVRTGRPVFDPATADLMDFRTPQPARGLSFGYVLPLDPRTALVEYTEF
                     SPAPLDTDGYRRALDHYTHDVLGIGPFDVTAREHGVIPMTDGRFPLRQGRSVYRIGTAG
                     GATRPSTGYTFAAVQRQSRAVADQLRSGRPLRRAHPYGRRARMMDAVLLRALDSGRVDG
                     ADFFDPLFRGVPGERLLRFMDGGSPPHEDLLIGLRTPVVPMLRTVVELPFRPRREHPAP
                     PLAPSSTDTEESTP"
ORIGIN
        1 gatctggaac aggttgcagg aggaggggtc gcagttcaat agttttctga cagatcgtca
       61 gattgtttcg tcgccacccc ttcccagttg tgccgcccgg cgctctaatg acggccgagc
      121 cgccagtcac ccggggtcag gaggaacggt ggagtacaac cttgccgacc tgttcgaatc
      181 ggtcgtcgac gcggtgccgg accgcgaggc gctcctgtac gtcgaccatc ccggcacggg
      241 cgcggagcgg cggctcacgt acgcggagct ggacgccgcc gccaaccgga tcgcccacca
      301 cctgatcgac gcggggatcc ggccgggcga gcacgtgggg ctgcacctct acaacgggat
      361 cgaatacctc cagacggtac tgggcgccct caaggcgcgg atcgttccgg tgaacgtcaa
      421 ctaccgctat gtcgaggagg agttggtcta cctctaccgg gacgcggacc tggcggccct
      481 ggtcttcgac ggcgagttcg acgagcgggt ggcggcagcc gcgccgcggt gcccgggcct
      541 gcgacacctg gtccgggtgg gggccgcccc cgcctctccc ccgggcgtcc cggtcgtgcc
      601 gttcgccgag gcggaggccg cgggcgcggc cgggcgcggc ttcggccccc gctccgccga
      661 cgaccagttc atcatctaca ccggcggcac gaccgggatg ccgaagggtg tgatgtggcg
      721 tcaggaggac ctcttcttct cgggcctggg cggcggggcc ccgaccggcg acccggtgca
      781 ggctccgcgg gagctggcgg agcgggtcgc ggcgggcggc gacgggatca ccttcttccc
      841 caccccgccg ctgatgcacg ggacctccac actgacggct ttcatcggct tcaacttcgg
      901 ccaacgcgtc gtcgtgcacc gcaagttcgt acccgccgag gtgctgcgga ccatcgagag
      961 ggagaaggtg accagtgtct cgctggtcgg cgacgcgatg ctgcgcccgc tgatcgactg
     1021 cttgaagggg ccgttacgcg gaacggactg ctcgtcgctg ttctccgtct ccagctccgg
     1081 ggcgatcatg tcggattcgg tgcgggcgga gttccaggag ctcgtgccga ccgtgctgct
     1141 gctgaacaac ttcggctcct ccgagtccgg tttcaacggc acggcgacgg cggactcggg
     1201 cccggagcgc ggcttccggg tacaggtcaa cgcccgtacg gcggtggtcg atccggtgac
     1261 gtacgagccg gtggccccgg gcgagccggg ccgcatcgcc cagcgcggtc atgtcccgct
     1321 cggctactac aacgacccgg ccaagaccgc cgacaccttc ttccgccggg gcgaggagcg
     1381 gtgggtgctg ctgggcgaca tggcgaccgt ggacgcggac ggcatcgtca ccgtgctcgg
     1441 ccggggctcc cagtgcatca acaccggcgg cgagaaggtg tatccggagg aggtcgagca
     1501 ggcgctcaag tcccatccgg acgtgtacga cgcgctggtc gcgggcgtac ccgacgagcg
     1561 ctggggcaac cgggtcgcag ccgtggtcca actccgcgag cacgcacggg gtctgacgct
     1621 cgacgaggta caggcgcact gccgcacccg gctggccgga tacaagatcc cccgcgccct
     1681 ggtgctcgcc gaccggatcc agcgctcgcc cagcggcaag gcggactacc gctgggcgaa
     1741 ggcggtggcg gcgggagcgg gccgggcctg aggttccgcc gccggcttcg ctgcgaacga
     1801 acggggaggg gcgggcacca cgacggcacc acggggccgg ggatcggccg gggccggcac
     1861 cgcgagaggc tgcccgggcg ctgagggtca aggcccggtc gccgatcgtc aaggacggaa
     1921 cggagagggg tccgcagact ggtgatggtc gggccgcgcg gcgtcacggg ggtgcgccgt
     1981 gcgcccgacc ccacgctgcc cgagggcgcc ggaaggttcg cgcctctcgc ggaccccacc
     2041 cgtgcgcacc cgcccgctcc ggctcgggcc ggggccgcac gaccgcctcc ggggtgcggc
     2101 tgccgggacg gggcggcgta cggccgttca cgtgggccat acgggcgtgg tggccccgtc
     2161 acggcgcgtc cggggtgttt ctccgccggg agtacggggc cggcgtgatc gtctctgcat
     2221 cagggccctt cttcgcgcgg cgcggactcc cgatcggcag gacgcggagg cgggccgtcg
     2281 ggtacgtgcc cgggcggagt gacgagccgt tcgtcagcac cactgatctc ggagggcgtg
     2341 agatgggccg tcgccagcct gaggaaccct cgttcggcga gtccgcgcgg ccggctcccg
     2401 ggacgggcgg gcaccgccac cacgccgccc cctcgtccga ggacgcccgg gtcgtggacg
     2461 cggacgtggt cgacgccgtc ctgcgcaccg cgcgggcggt gctggcggag cgggtggacg
     2521 aggcgatggc gagcgacgct ctgttctgcg acgagctggc acggcgggtc gccgatttca
     2581 cgctcgacgg cggcaagcgg gtacggccgc gcctgctgtg gtgggggatg cgctcctgcg
     2641 gtgcggcgga taccacggcg gcgctgcggc tcggggtggc gctggagctg atccagagct
     2701 gtgcgctgat ccatgacgat gtgatggacc gctcgcggct ccgccggggg aagcccgccg
     2761 tgcacatcgg tctggcggaa cgtgcgggtt actcgccgga ttccgggcgg ggttccgcct
     2821 tcggcgcctc ggccgccgtg ctggcgggtg acctcgcgct ggcctgggcc gacgacacgg
     2881 tggcggagac cgcgctgtcc gccgtccagc gtcgtcggat cggccccctg tggcggacca
     2941 tgcggaccga gatggtcgcc ggacagtacc tggatctgcg cggccaggtc cgtggcgggt
     3001 catccgtgga gcaggccctg cggaccgcgt gcctcaagag cgcctcgtac tccgtcgagc
     3061 ggcctctggc gctcggtgcc gccctggcgg gcgccgatga agcgaccacc gacgccctgc
     3121 gctccgccgg ccggtgcgcc ggcatcgcct ttcagctgcg ggacgacctg ctcggggtct
     3181 tcggcgaccc ggagctgacc gggaaaccgt ccggcgacga catccgcgag ggcaagtcga
     3241 cctacctgct ccccgtcgcc cggaggtggg cggaaccggc gggcaacaaa caatctctgg
     3301 cggtgctcgg ccgcttcccg ggcggcggca acctcgccaa ggaggatctc ccggaggtac
     3361 gcggcgtgct cgaggagacg ggggcacgcg cccacgtgga gcgcaaggcg gaccccctcc
     3421 gcgaccacgc gttgggccgt ttggcacagg ccgttgaagt ggaagcgcac ggcggccgcc
     3481 agttgctcgg actgctccgc acggtttccg gagagcggac cgggcaatcc cccgcaaccg
     3541 gcgggacgca cggtcacggc cccggggcgg cagccgccgc cccggccctg accacggcgg
     3601 aaggaggtac caccaggtga tcacggtcaa ggggcgcgtc gaccacgtgg tggtggtcgg
     3661 cgccggtctg gcggggctgt ccgccgcgct gcacctgctg ggcgccggcc gccgggtgac
     3721 ggtcgtcgag cgggatagcc tgcccggggg ccgggccggg ctgctggaat cgggcggctt
     3781 ccgtatccac accgggccga ccgtgctgac gatgccggac ctggtggagg aggccttcgc
     3841 cgcggtcggg gaggctacgg ccgaccgctt ggagctgatc aggcccgccc ccgcctaccg
     3901 ggcccggttc gccgacgggt cccggctcga cgtgcacacc gacggcgccg cgatggaggc
     3961 agccgtcgag gagttcgcgg gggcccggca cgcggccggc taccggcggc tgatgatctg
     4021 gctggagcgc ctctaccggg cgcagatgcg ccgtttcatc gacaccaact tcgactcacc
     4081 gttccagctt gtccacccgg acctcgcccg gctcgccgct ctcggcggtt tcggccggct
     4141 tgacgcgcgg atcgggcact tcgtctcgga cgaacggctg cgcagggtgt tctccttcca
     4201 ggcgctgtac gcgggtgtgc ccccggcacg ggcgctggcc gcgtacgcgg tgatcgccta
     4261 catggacacg gtggccgggg tgtacttccc tcggggcggc atgcacgcgc tgccccgtgc
     4321 gatggcggac gcggcagccg acgcaggagc gtccttccgc tacgggcaga gcgtgacccg
     4381 cctggagcgc tcgggcgacc gtgtcaccgc ggtcgtcacc gaccaggagc gcattccgtg
     4441 cgacgcggtc gtgctgaccc ctgatctccc ggtcagctac cggctgctcg gccggagccc
     4501 gcgccgcccc ctgccgctgc gacatgcgcc gtccgcggtg atcctgcacg cgggcacgga
     4561 ccgcacctgg ccggagctgg cgcaccacac catctcgttc ggcgcggcct ggaagggcac
     4621 cttccacgag ctcacccgcg ccgggaagcc gatgtcggac ccctcgctcc tcatcacccg
     4681 gcccacggcg agcgacccct ccctcgcgcc gccgggcaaa cacctccact acgtgctggc
     4741 tccctgcccg aacaccgagg tcgggcccgg cgtacgggag tggcgggagc tgggaccccg
     4801 ctaccgggac cagctggtgg ccgaactgga gcgccgcgac atgcccggtc tcggcgcggc
     4861 gatcgaggag gaggtgctgg tcacgccggt cgactggacc gcgcaggggc atgcggcggg
     4921 cacccccttc tccgtggccc acaccttccc ccagaccggc cccttccggc cgcgcaacct
     4981 ggtgcggggc acggccaacg ccgtcctcgc cggttgcggc accacacccg gtgtcggcgt
     5041 gccgaccgtc ctgatctccg ggaagctggc ggcggagagg atcaccggcc cccgcgccac
     5101 ccgcaccccg cgccccaccc cgggaggttc ccccgcatga ccgtccgcga actcgacgcc
     5161 gcaggcatcc acgaccccgc gctccgggcc gcgtacaccc gctgccgcgt tctcaacgca
     5221 cgtcacggca ggacctactt cctcgcgaca cggctcctcc ccgtcgaccg ccgtcccgcc
     5281 gtccacgccc tgtacggctt cgcccgccgc gccgacgaca tcgtcgacga tctcgacagc
     5341 agcgccacac cgggggaacg cgcccgcgcc ttgctcgacc tggaggcccg gctggaggcg
     5401 gggctgcggg gggccccggc cgccgagccg gtgatccgtg ccctggccga cacggcgggc
     5461 cggtacgcca tcgaccatcg ccacttcacc gatttcctcc gctccatgcg gagcgacctg
     5521 accgtcggcg gctatgcctc gtacgaggaa ctcggccggt acatgcacgg ctcggccgcg
     5581 gtgatcggcc ttcagatgct gccggtgctg gggacggtgg tgccgcgcga ggaatcggct
     5641 ccgcacgcgg ccgcgctcgg tgtcgccttc cagtggatca acttcctgcg cgacgtcggt
     5701 gaggacgtgg acccggaacg cctctatctg gcgaccgagc tcctgggcgc gcaccgggcc
     5761 gaccgagacc gcttcgagtc gagccgaagc acggggatgc cggacagccg gatcaccgat
     5821 gtcctgccgg ccgccgcagc ccactaccga gccgtctacc gggctgccct gcccggcatc
     5881 gcgatgctcg ccccggtgtc gcggccctgc atccgtaccg cgttcgtgct gtacagcggc
     5941 atcctcgacg cgatcgaggc cgacgggtac gcgctcctgc accgcccggc ggtcgtcccg
     6001 cgccgacgac cggccgccgt gccgctggac gatctggtgc cggcggtcgc cgtgcgcacg
     6061 ctgtgccggc gcccagcgca cgcggtggat gcgtcccgga ccgccgagct gtccgggccg
     6121 cggacggcgg gccgacccgc ccgggtgcgg acggaggccg tatgaggccc gcgggacgac
     6181 gccgacgcac cggccgtcat cccttgccgg tgcggcgggc gcccgacccg tggcagcgtc
     6241 cggcacgcac gaggcgcgac gcgcaaccct cgctcaccac ggacgccctg gagcgtgcgc
     6301 cgagcacgcc ttccggcaac tggtacgtca tcggagcctc gcgcgccctt cctcccggga
     6361 aggccctcgg ggtcacggtc ggctccaccg aagtggtggc gtggcgcgac ggcgcgggaa
     6421 agctgcgcac cggcccgggt gcgtgcccgc atctgggggc accgctgcgc gacagccccg
     6481 tccgctgcgg cacgctggtc tgccactggc acggactcgc gctggacggc gggccgttcg
     6541 ccggatggga gccctatccc gccttcgacg acggggtcct gctctgggtg cggctcgacc
     6601 gggagggggg tgagccccct tcacaacgtc cccgcgtacc ctcccggccg agggttcggg
     6661 acgccgtgga ggcggtctac tgcggcgtgg gccgttgtga gccggaggac gtggtggcca
     6721 accgcctcga tccgtggcac ggggcgtggt tccacccgta ctccttcgtg gacctgacgg
     6781 tgacgggcac gcccgaggac ggcccggcgg acggcgacgg gttcaccgtc gacgtgtcct
     6841 tcaaggtggc cgggcgcctg gtggtgcccg tccgggcccg gttcacggcg cccgggcccc
     6901 gtacggtcgt catgcacatc gtcgagggcg agggcgcggg gtccgtcgtg gagacccacg
     6961 ccacccccct cgctccggac cggtggggca agccgcgcac ggcggtgacg gaggccctgg
     7021 tcgcggcctc ggaccgtcgc ggattcgccg tggcccgggc ggcggctccg ctgttgcgtc
     7081 ccctgatgcg cgcctcggcc ggccgcttgt ggcgggacga cctggcgtac gcggagaggc
     7141 gctgggaact ccgcgaccgc gggacgttcc ccggctgacg gccggccggc cggccaaggc
     7201 tccgcgccgc ccctccgtca gcctccgtca gtcggccacc gcggcaagcc tgcgcaggac
     7261 ggccgaccgc cccgcgcgcg ggacggtcca aagcgtctgc ccgcttaccc cccagcgctc
     7321 cagcagggcg ttcgcggcga ggaatccgct ggtggccgct cgttccatca gagccacggg
     7381 cagcccggtg cggaccatgt ccccggccac ggtgaccgtg gggtcggggc tgcgcacacc
     7441 gggccggtcc cggtagccgc cgaccgggaa catggggcag tccgcccgcc actcgtgacg
     7501 ctcgtccagg agccgggcgc tccgggtctc cgggtagacg cggtgcagtt gcccgagcgt
     7561 ctcggcctgt acggcggccc ggtcggcgtc cggtgcgacg gcgtaggcgt gcagttcgac
     7621 gaccgagccc cgcgtgcgcc gggcccagcg ggcggcctct ccctcccagc ggtcgaggac
     7681 gctgacgttg tccagcgggc cgtagccgct ggtgcccagg aaccccggcc ggtcgtgcgc
     7741 cacgggccgg tcgagccaga gccgtgagac caggaagggc ggggcggtgc gcagccgggc
     7801 gatccggtcc cgccagtcgg cgtcgcccaa ctcgggggac gcggcgacga ggtggcgcag
     7861 tccaccgctg tccagggcca ggacgagggc gtcgtaccgc gtggtcaggt cgtccgtcgt
     7921 gaggaccgcc ccgccctcgg gcagggggcg gacctcgcgg accggcgtcc gtgtccgtac
     7981 gtcgacgccg tgcccttcca ggtagcggtg gagcgggtcc cacagggccg cggggaacgt
     8041 ttcgcggggc acgtcgaaga tcaggccctc ggcggagccg aggaagtaga tgtggaacat
     8101 cagcgccagt tcggcagcgg acagttcacg cgggtcggcg aagaaactgc gggagaagac
     8161 ctcgaaggcc aggtggtggg cggcctccgg gaagcggatg cggtggagga agtcggtggc
     8221 gctgatcccg tcgagccgtt cgtagacctc gggcacccgc acgtcgagca gccccagagc
     8281 cgccctcggg ttcatccgga ccaggtcccc gggaccgaag gtggggctga gggcgacgaa
     8341 tcccagggcg ctgagcggag gcgtgcgcgg cacccgggcg aagccgtccc gcaggccgga
     8401 gctgtgccgc agcggatagt cgggcagccg ggtcagggat ccgaggccgg ggtcgacccg
     8461 acgcagcagt ccgcgcaggt tgtagtactg ccggaagaac gcgtggaacc ctcggctcat
     8521 cgtcaccgtg gtgccgtcgg cgaggcgggt gggccacccc gccagcctcc cgccgaggcc
     8581 ctcttcgctc tcgtggagag tgacgcgtgc tccgcgttcg gccagcaggg tggccgccgc
     8641 gatccccgcg atgccgccgc cgacgaccgc caccgcgggg gcgtcccccg tgaaccgggc
     8701 acggccggcc ggggccggaa cgacctccgc ccgccggtcc cgcccgtgcc ggtgcccccc
     8761 gtcgccgtcc cgccggagca tcatgcccgt ccccccgggc cggcggcggt gcgggcgacg
     8821 aaggtgtgcg tgatgccggt ctgccagccg ggcagcggga gcacgcgtac gtcggtgaag
     8881 ccggcccggc gcagccgccg ggcgaacgcg ggagccgtgt cgaaccgggt gacgctgtcc
     8941 cggaggtgtc ggtagaggcc ggtgtccccg gcgagccggc cggccggctg gatgatcagg
     9001 gagcagatcg tcctccacgc ggccaggtgg gcgggggaac cgccgagcgt gtactcgtgg
     9061 acggcgagcc ggccaccggg gacgagcaga ccgcgcacca ggtcgagggc ctggtcgggg
     9121 tcggcgaggt tgcggaagag gtatccggcg aacaccgcgt cgaaggaacc ggcttccagc
     9181 cgtccggcca gctcctcgac cgggtggtgg gcgaagtcga cgccctgcgg ccagctcttg
     9241 gccgtcgccc gggccagcat gcccgcggac gcgtcgaccc cggtgatccg ggcgagcggg
     9301 gcggcggcga gaagcgcggc cgtggaggcg cccgtgccgc atcccaggtc gagcacgcgc
     9361 agcccgcgtc ccccctcggg caggcccagt cggcgtgcgg agcggcgcag gtgcgcgtgg
     9421 tagccggggc tggccgcgac gaggcggtcg taggcggcgg agccgtggtc gaaggcctcg
     9481 gacagtgcgt caccgcgcaa cagggtcatg gggtgctctc ctcggtgtcg gtcgatgagg
     9541 gggcgagcgg gggcgccggg tgttcccgcc ggggtcggaa agggagttcg acgacggtcc
     9601 gcagcatggg gaccaccgga gtccgcaggc cgatgagcag gtcctcgtgc ggtggcgaac
     9661 cgccgtccat gaagcgcagc agccgctcac cgggaacgcc ccggaagagc gggtcgaaga
     9721 agtccgcgcc gtccaccctg ccgctgtcca gggcccgcag cagcacggcg tccatcatcc
     9781 gcgcccgccg cccgtacggg tgggcccgac gcagggggcg tccggagcgc aactggtcgg
     9841 cgacggcccg gctctgtcgc tgcacggccg cgaaggtgta gccggtcgac gggcgggtcg
     9901 cgcctccggc ggtgccgatg cggtacaccg aacgcccctg cctgagcggg aagcgcccgt
     9961 ccgtcatggg gatgacgccg tgctcccgcg ccgtgacgtc gaacggcccg atccccaaca
    10021 cgtcgtgggt gtaatggtcc agggctcgcc ggtagccgtc agtgtccagc ggggcgggtg
    10081 agaactcggt gtactcgacc agcgcggtgc gcgggtccag cggcaggacg tagccgaacg
    10141 agagcccgcg cgcgggctgc ggtgtgcgga agtccatcag gtccgcggtc gccgggtcga
    10201 acacgggacg cccggtgcgg acgaaccagc cggtgaaatg ctgcagcagc gtcgtacggg
    10261 cgggcggcag gcggcggggc ggccgggagt cgaagacgag acggccggtc acgaggacgc
    10321 gttccccgcc cgggccccgg gtcaggacct caccgcgggc gccggacggg tcgtcgcgta
    10381 cggcgctcgc ggtcgcttcc atccggcaca ggcgcggtgc gcgggcgagc cgtcgctcga
    10441 ccagcgcctc gaaagcgtcc gagcacagca tcttgtagcg cagccgtgag agccgggcca
    10501 ccgtggcggc tccgtcggcc gcccggatcc gcagccgggg ccaggaagcg gccaggctct
    10561 gatcgtaggg cccgcccggc ggctcccaga agcaccaggt ccgcgggggc gcccgcagag
    10621 gccccggcgg ggcgtcgacg agggcgaccg acacgggtac gtcggcgccc ggtgcgcaaa
    10681 ggtggtacgc caggctcatc ccggccgcgc ccgctcccac gatcacgacg tcgaagtcgg
    10741 cgggcacggc gttccctctc ggtcggacgg gtgcgcaccc tcgcggtccg ggcggagccg
    10801 gcgccccgca catggcgaag cggggcgggc cgggtgccag gcgaccgtat ccgctgcgcc
    10861 ctctcccacc cggcatgc
//
