BGC0000652 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000652 (Streptomyces sp. CNQ-525)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	other,T3PKS,terpene-precursor	1	36,405	High	napyradiomycin	terpene

BGC0000652 - Region 1 - T3PKS,other,terpene-precursor

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Location: 1 - 36,405 nt. (total: 36,405 nt) Show pHMM detection rules used

Region on contig edge.

other: (Neocarzinostat or bcpB or frbD or mitE or prnB or CaiA or bacilysin or orf2_PTase)
T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)
terpene-precursor: (PT_FPPS_like and not PT_HPP)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

Terpene

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 36,406
Label		BGC0000652

Tailoring functions

Oxidoreductases: Halogenation Oxidation

napB2: Oxidation

MITE: MITE0000084Oxidation (identity: 99.50%)

napH1: Halogenation, Heterocyclization

MITE: MITE0000086Halogenation, Heterocyclization (identity: 96.20%)

napH4: Halogenation, Heterocyclization

MITE: MITE0000086Halogenation, Heterocyclization (identity: 72.00%)

Transferases: Halogenation aminotransferase halogenase methyltransferase

napB3: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

napB5: Methylation

MITE: MITE0000085Methylation (identity: 99.70%)
smcogs: SMCOG1042: O-methyltransferase

napH1: Halogenation, Heterocyclization

MITE: MITE0000086Halogenation, Heterocyclization (identity: 96.20%)

napH2: conventional flavin-dependent halogenase

halogenases: conventional flavin-dependent halogenase
smcogs: SMCOG1119: halogenase

napH4: Halogenation, Heterocyclization

MITE: MITE0000086Halogenation, Heterocyclization (identity: 72.00%)

Ligases: ligase synthase synthetase

napB1: chalcone and stilbene synthase domain protein

smcogs: SMCOG1024: chalcone and stilbene synthase domain protein

napB4: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

napT7: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000652.5	2.52	terpene	napyradiomycin	Streptomyces sp. CNQ525
BGC0001079.5	1.62	PKS, terpene	napyradiomycin A80915C, napyradiomycin 2, napyradiomycin 3, napyradiomycin 4	Streptomyces aculeolatus
BGC0002341.2	1.38	other (other)	3,5-dibromo-p-anisic acid	Planctomycetales bacterium 10988
BGC0002608.2	1.35	NRPS, PKS	ochratoxin A	Aspergillus steynii
BGC0002127.3	0.77	PKS	flaviolin, 1,3,6,8-tetrahydroxynaphthalene	Streptomyces coelicolor A3(2)
BGC0001310.5	0.76	PKS	naringenin	Streptomyces clavuligerus ATCC 27064
BGC0000902.4	0.74	PKS	flaviolin	Saccharopolyspora erythraea NRRL 2338
BGC0000285.5	0.67	PKS, saccharide	flaviolin, flaviolin rhamnoside, 3,3'-diflaviolin	Saccharopolyspora erythraea
BGC0002561.3	0.55	other (other)	phenazine SA, phenazine SB, phenazine SC	Streptomyces sp.
BGC0000280.5	0.50	PKS	2,4-diacetylphloroglucinol	Pseudomonas fluorescens

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000652.5	1.00	terpene	napyradiomycin	Streptomyces sp. CNQ525
BGC0001079.5	0.87	PKS, terpene	napyradiomycin A80915C, napyradiomycin 2, napyradiomycin 3, napyradiomycin 4	Streptomyces aculeolatus
BGC0002341.2	0.76	other (other)	3,5-dibromo-p-anisic acid	Planctomycetales bacterium 10988
BGC0002608.2	0.75	NRPS, PKS	ochratoxin A	Aspergillus steynii
BGC0002127.3	0.59	PKS	flaviolin, 1,3,6,8-tetrahydroxynaphthalene	Streptomyces coelicolor A3(2)
BGC0001310.5	0.59	PKS	naringenin	Streptomyces clavuligerus ATCC 27064
BGC0000902.4	0.58	PKS	flaviolin	Saccharopolyspora erythraea NRRL 2338
BGC0000285.5	0.55	PKS, saccharide	flaviolin, flaviolin rhamnoside, 3,3'-diflaviolin	Saccharopolyspora erythraea
BGC0002561.3	0.50	other (other)	phenazine SA, phenazine SB, phenazine SC	Streptomyces sp.
BGC0000286.3	0.48	PKS	viguiepinol	Streptomyces sp. KO-3988

Similar known clusters from MIBiG 4.0

Detailed terpene predictions

Product predictions:

Class	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor	FPP	C15	acyclic	PP
diterpene precursor	GGPP	C20	acyclic	PP
sesterterpene precursor	GFPP	C25	acyclic	PP
triterpene precursor	HPP	C30	acyclic	PP

Genes with terpene domains:

napT7: PT_FPPS_like

Domain 1 (59 - 335): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS substrates

Terpene

NRPS/PKS substrate predictions

napB4: X

AMP-binding (41..432): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: SRAESTIPAK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

Link to PARAS substrate predictor

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate