Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0000654.1:
Cluster 1Terpene / Saccharide149461

BGC0000654, phenalinolactone biosynthetic gene cluster from Streptomyces sp. Tu6071. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: Phenalinolactone A
ChemSpider ID: 28288564
SMILES string: Copy to clipboard
Molecular formula:
Average molecular mass: Da
Molecular activity: Antibacterial

Class-specific details

Biosynthetic class(es):
Terpene / Saccharide
Terpene subclass:
Other
Terpene subclass by size:
Diterpene
Final isoprenoid precursor:
IPP
Terpene synthase/cyclase-encoding genes:
ABB69742.1, ABB69743.1, ABB69754.1, ABB69755.1, ABB69756.1
Prenyltransferase-encoding genes:
ABB69744.1
Saccharide subclass:
hybrid/tailoring
Glycosyltransferases:
ABB69761.1:
Specificity: L-amicetose
Evidence for specificity: Knock-out construct
Sugar biosynthesis subcluster: ABB69735.1, ABB69736.1, ABB69737.1, ABB69738.1, ABB69760.1, ABB69762.1, ABB69763.1

Gene cluster description

phenalinolactone (BGC0000654). Gene Cluster 1. Biosynthetic class = Terpene/Saccharide. GenBank DQ230532. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies, Heterologous expression
MIxS-compliance:Unknown
Contact for this cluster:Andreas Bechthold (Albert-Ludwigs-University of Freiburg, Germany)

Literature references

1. Durr C et al. (2006) Biosynthesis of the terpene phenalinolactone in Streptomyces sp. Tu6071: analysis of the gene cluster and generation of derivatives. Chem Biol 13(4):365-77. doi: 10.1016/j.chembiol.2006.01.011.
2. Binz TM et al. (2008) Heterologous expression and genetic engineering of the phenalinolactone biosynthetic gene cluster by using red/ET recombineering. Chembiochem 9(3):447-54. doi: 10.1002/cbic.200700549.
3. Daum M et al. (2010) Functions of genes and enzymes involved in phenalinolactone biosynthesis. Chembiochem 11(10):1383-91. doi: 10.1002/cbic.201000117.
4. Gebhardt K et al. (2011) Phenalinolactones A-D, terpenoglycoside antibiotics from Streptomyces sp. Tu 6071. J Antibiot (Tokyo) 64(3):229-32. doi: 10.1038/ja.2010.165. Epub 2010
5. Myronovskyi M et al. (2011) Beta-glucuronidase as a sensitive and versatile reporter in actinomycetes. Appl Environ Microbiol 77(15):5370-83. doi: 10.1128/AEM.00434-11. Epub
6. Kiske C et al. (2014) Metabolic pathway monitoring of phenalinolactone biosynthesis from Streptomyces sp. Tu6071 by liquid chromatography/mass spectrometry coupling. Rapid Commun Mass Spectrom 28(13):1459-67. doi: 10.1002/rcm.6920.