LOCUS       BGC0000657              5951 bp    DNA     linear   BCT 18-JUL-2011
DEFINITION  Pseudanabaena sp. dqh15 2-methylisoborneol (2-MIB) synthesis
            associated operon, complete sequence.
ACCESSION   BGC0000657
VERSION     BGC0000657
KEYWORDS    .
SOURCE      Pseudanabaena sp. dqh15
  ORGANISM  Pseudanabaena sp. dqh15
            Bacteria; Cyanobacteriota; Cyanophyceae; Pseudanabaenales;
            Pseudanabaenaceae; Pseudanabaena.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 13:59:48
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..5951
                     /country="China: Lake Dongqian"
                     /db_xref="taxon:1002505"
                     /mol_type="genomic DNA"
                     /organism="Pseudanabaena sp. dqh15"
                     /strain="dqh15"
     operon          1..5951
                     /operon="2-methylisoborneol (2-MIB) synthesis associated"
     protocluster    1..5951
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="True"
                     /core_location="[3097:4291]"
                     /cutoff="20000"
                     /detection_rule="(Terpene_synth or Terpene_synth_C or
                     phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7
                     or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      3098..4291
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(Terpene_synth or Terpene_synth_C or
                     phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7
                     or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
     cand_cluster    1..5951
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Terpene_synth or Terpene_synth_C or
                     phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7
                     or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..5951
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000657"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..5951
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="terpene"
                     /region_number="1"
                     /rules="(Terpene_synth or Terpene_synth_C or phytoene_synt
                     or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps
                     or fung_ggpps2 or trichodiene_synth or TRI5)"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            644..2044
                     /gene="cnbA"
                     /operon="2-methylisoborneol (2-MIB) synthesis associated"
     CDS             644..2044
                     /codon_start=1
                     /gene="cnbA"
                     /operon="2-methylisoborneol (2-MIB) synthesis associated"
                     /product="cyclic nucleotide-binding protein"
                     /protein_id="AEK21531.1"
                     /transl_table=11
                     /translation="MTENLDSNQQMLAERSSLSTRAAKQLATTTKTAPQMQGITSRWIS
                     RLLPWVELRGGSYRVNRRLTYALGDGRVTFTNVGSTIQVIPQELRELAFLRDFEDEAVL
                     NALADRFVQQEFQPGDVLVDKGTPADQIVLIAHGKVSKIGTGKYGDEVELAVLTNGDHF
                     AYQAILESEDLWDFTIKAITRCTVLALTQSSFEELVNSSESLQAHIEAFRTQPQKDQDR
                     YGQSAIDLAAGHSGEPVLPATFVDYETSPREYELSLAQTVLRVHTRVADLYNEPMNQIA
                     EQLKLTVHELRERQEYELVNNREFGLLHNADLGQRFSTRNGPPTPDDFDELITRRRNAQ
                     YIFAHPRAIAAFGRECSKLGLYPDSIDMGGERVLTWRNIPILPCDKIPISNTQTSSVIV
                     VRTGEDNQGLVGLRPAALPDQHEPGLNVRFMGINEKAIISYLVSNYFSAAILVPDAVGV
                     LEDVELGR"
     gene            2133..3002
                     /gene="mtf"
                     /operon="2-methylisoborneol (2-MIB) synthesis associated"
     CDS             2133..3002
                     /codon_start=1
                     /gene="mtf"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089:methyltransferase (Score: 69.7; E-value:
                     4.3e-21)"
                     /gene_kind="biosynthetic-additional"
                     /operon="2-methylisoborneol (2-MIB) synthesis associated"
                     /product="SAM-dependent methyltransferase"
                     /protein_id="AEK21532.1"
                     /transl_table=11
                     /translation="MSTPQTITADNVLRSEYQKSVANYWNQEKNPVNLLLGHVDGYYHH
                     HYGIGDVDWSVLEAPEGERDDQIIRELHRLEHAQAVFLLDHLGNVKPDDRVMDGGSGRG
                     GTSFVVNERFGCQVDGVSISESQVKFANDQSVQRGIADKVKFHFRNMLDTGFETGAMRS
                     IWTNETTMYVDLFRLFAEFSRLLQDGGRYACITGCYNDVTGGRSRAVSQIDAHYICNIH
                     PRSQYFKALAANNLVPITVTDLTPLTIPYWELRAKSSLITGVEDYFLTAYKEGSFHYLL
                     IVADRIIR"
     gene            3098..4291
                     /gene="mic"
                     /operon="2-methylisoborneol (2-MIB) synthesis associated"
     CDS             3098..4291
                     /codon_start=1
                     /gene="mic"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: Terpene_synth_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1052:Terpene synthase/cyclase metal-binding domain
                     protein (Score: 203.8; E-value: 7.2e-62)"
                     /gene_kind="biosynthetic"
                     /operon="2-methylisoborneol (2-MIB) synthesis associated"
                     /product="monoterpene cyclase"
                     /protein_id="AEK21533.1"
                     /sec_met_domain="Terpene_synth_C (E-value: 1.7e-09,
                     bitscore: 25.5, seeds: 87, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKDTNLDNTSTTFLFGPTGLGTSAARFVSEIAKAARTDNLDSGLL
                     QTDLASVGDCDPAYAERDWGDGTASPLYCPITERFNEPLADEVDDRLADWALECGFDED
                     EAQKIRKVRFGRLVMLAHPDCDDPSRLLIGAKLNMAWWAADGYYADDTELGADPKLLPP
                     RLLLAMTAMDPPPPAGEFTPPMEAAIAEERVLVALGKGIDYLSQYATPEQVQRTCYATF
                     FMFVSWSAYAAWRYTGEYPPAWKYLAARQHDSFYTSMTLIDPIGGYVLPPDLFFDPRVR
                     HTAFLAGTAVVLVNDLLSVAKDLADEQPPVNMVLQIAADRGCSIEEATEITVKFHNDLV
                     HDFRERHQKLQAVPNVELQRFLRGLRGWMGGAFEWHNSNPRYKNSNGASQPDSQSLA"
     gene            4377..5774
                     /gene="cnbB"
                     /operon="2-methylisoborneol (2-MIB) synthesis associated"
     CDS             4377..5774
                     /codon_start=1
                     /gene="cnbB"
                     /operon="2-methylisoborneol (2-MIB) synthesis associated"
                     /product="cyclic nucleotide-binding protein"
                     /protein_id="AEK21534.1"
                     /transl_table=11
                     /translation="MTQDFNSHGLAEQSSLSTDAARKLATTTKTTPQTQGISSRWISRL
                     LPWVELRGGSYRVNRRLTYALGDGRVTFTNVGATIRVIPQELREIALLRDFDEEAVLNR
                     LAERFVQQEFQPGDVLVEKGTPADQIVLIAHGKVSKIGTGKYGDDMELTVLTDGDHFTY
                     QAIIESADLWDFTMKAVTRCTVLVLTQQSFEDAVASSESLQAHIEGFKARPPKAQDRYG
                     QSAIALAAGHTGEPVLPTTFVDYETSPREYELSLVQTVLRVHTRVADLYNDPMNQIEQQ
                     LKLTVHELRERQEYELVNNQEFGLLHNADLSQRFPTRNGLPHPDDFDELITRRRNSQYI
                     FAHPRAIAAFARQCNKMGIDLESIDVGGNRIPAWRGIPIYPCGKIPIDRKTQTTSIIVM
                     RTGEDNQGVVGLQPAELPDQYEPGLNVRFMGINEKAIISYLVSNYFAAAILVPDAVGIL
                     EDVEIGR"
ORIGIN
        1 cgagtacaaa gtgcgaatat ttgggtgaga tgacatatcg ctttcaggat gactatgcga
       61 gacatgagaa aaagtaactt aagtagctgg tgtaattaaa tacaaaatgt gtctgtgtgg
      121 aacgagcatc ttgcccatca acaggctaga agcctgttcc acaatctttg aaggtcttgt
      181 atctgcccca tgagagtcct tcatgatcat cataacttca ctcagttcat tcttaatcaa
      241 ctttttcttt ctcctgtcaa tgatggcagc atcacttgcc gttgacagtg ttgaatttga
      301 aactgctgtt tttctgcttc ctacctcctt ggttcgcaga gtaacagaag agagctataa
      361 tctcctgcca gctaggggga cgcaatcgat tttgaattaa tattaagact cggttcaatt
      421 ggcattatat tttagtacag tcaaatttcg atatagcaaa tgtcaacacg gtctaagatc
      481 atcgcaacca tccaaggggg ggtaggttgt tgtacttgag gctcatcaaa tgacttaaag
      541 taagacaaat cagtgttaga tcacatcaaa aattgctgga ataacctttg cttgctcacc
      601 ccttggataa gtacaaacca tcaatacaat cagagggtta aacatgacag aaaatctaga
      661 ttctaatcag cagatgctgg cagagcggtc tagcctgagt actcgggcag cgaagcagtt
      721 ggctacaacc accaagactg ctccgcagat gcagggcatc acatcgcgct ggattagtcg
      781 cctgttgcct tgggtggagc tgcgcggcgg ctcctatcgt gtcaatcgcc gcttgaccta
      841 tgcactaggt gatggtcgtg tcacttttac gaacgttggg tcaacgattc aagtaattcc
      901 tcaggagttg cgcgaattag cctttttgcg agactttgaa gatgaggctg tcttgaatgc
      961 cttggctgat cgctttgtgc agcaagagtt tcaacctggt gatgtgctgg tagacaaggg
     1021 aacccccgcc gaccagatag tactgattgc tcacggcaaa gtcagtaaga tcggtacggg
     1081 caagtacggc gatgaggtcg agcttgcggt gttgaccaat ggcgatcact ttgcctatca
     1141 agccattctt gaatcagaag atctatggga cttcaccatc aaagcgatca cacgttgtac
     1201 ggtcttagca ctaacacaaa gctcctttga agaactggtc aactcctcag aatcacttca
     1261 ggcacacatt gaggcatttc gcacccaacc ccaaaaagat caggatcggt acggtcaatc
     1321 cgccattgat ctagccgccg gtcacagtgg tgaacccgtg ctgcccgcaa cctttgtcga
     1381 ctacgaaacc tctccgcgtg aatatgagct aagcttggcg cagacagtac tgcgtgttca
     1441 tacccgcgtt gccgacctct acaacgagcc aatgaaccag attgcggaac agcttaagct
     1501 aacagtacac gagttgcgtg aacgtcaaga gtacgagttg gttaacaacc gagaatttgg
     1561 gctactgcac aatgctgacc tcggtcagcg cttctctacc cgcaatggtc cgcccacacc
     1621 agatgatttt gatgagctga tcacccgccg ccgcaatgca cagtacattt ttgcccaccc
     1681 gcgtgccatt gcagccttcg gtcgggagtg cagcaagcta ggtctctatc ccgacagcat
     1741 tgatatgggg ggcgaacgag tgttgacttg gcgcaacatc ccgattttgc cctgcgacaa
     1801 gattcccatt agcaacaccc agaccagttc tgtgatcgtt gtccgtactg gtgaagataa
     1861 tcagggctta gtagggcttc gccccgcagc attgcccgat caacatgagc cggggttgaa
     1921 cgtgcgcttc atgggcatca atgaaaaagc catcatctcc tacctcgtta gcaactactt
     1981 ctccgcagcc atcctcgtac ccgatgccgt tggcgtactc gaagatgtcg aactcggacg
     2041 ctagtagatc ccagtagcgg taacggaaca gccaactgtc agcattttca ccatacactc
     2101 attgaaacac ctattcacca gtaacacatt ctatgtcaac gccccaaact atcactgccg
     2161 acaatgtttt gcgtagcgag tatcagaagt cagttgccaa ctattggaac caagaaaaaa
     2221 atcccgttaa tcttctactc ggtcacgttg atggctatta ccaccaccac tacgggatcg
     2281 gcgatgtcga ttggtcggta ttagaggctc ccgaaggtga gcgcgacgat cagattattc
     2341 gtgaactaca ccgactcgaa cacgctcagg cggttttcct gctcgaccat ctgggcaacg
     2401 tcaagcctga tgaccgcgtg atggatggcg gttctggtcg cggcggtacc agctttgtgg
     2461 tcaatgaacg cttcggctgt caggtcgatg gagtgtcaat ctcagaatct caggtcaagt
     2521 ttgccaatga ccagtctgtg cagcgcggca tagctgacaa ggtaaagttc cactttcgca
     2581 atatgctgga tactggcttc gagacgggag ccatgcgtag catttggacg aatgagacca
     2641 caatgtatgt ggatctgttc aggctgttcg ccgagttttc tcgtcttttg caggatggtg
     2701 gtcgctacgc ctgcataacg ggttgctaca acgatgttac gggagggcga tcgcgggctg
     2761 tcagccagat cgatgcacat tacatctgta acatccatcc gcgcagtcaa tatttcaagg
     2821 cactagcggc gaacaacctc gtcccgatta ctgtaaccga tttgactccc ctaacgattc
     2881 cctactggga actacgggcc aagtcttcgc tcatcactgg ggttgaggat tacttcctta
     2941 ctgcatataa agagggcagt ttccactatc tgctcatcgt tgctgaccgc atcattcggt
     3001 aaggattatc caaaacataa cccgcaccat ggtgtggtgc gtagcacgca tactgcaaac
     3061 gcaccacaca tcactgccct aataaaggga taagagcatg aaagatacca acttggataa
     3121 tacgagtacc acatttttat ttggtccgac tggtcttggt acatccgccg ctcgctttgt
     3181 gagtgagata gccaaggctg ctcgtaccga taacctagat tcaggactac tccagacaga
     3241 tctcgcctcg gtgggggatt gtgatcctgc ctatgcagag agagattggg gcgatggaac
     3301 tgcctcacca ctctactgcc ccattaccga gcgattcaac gagccactag cggacgaggt
     3361 cgatgatcgg ctggcggact gggcattgga atgcgggttt gacgaggacg aggcgcagaa
     3421 aattcgcaag gtgcggtttg gacggctggt gatgctagct cacccagact gcgatgaccc
     3481 atctcggctg ttgattgggg ccaagttgaa catggcttgg tgggctgctg atggctacta
     3541 tgctgacgac accgagcttg gggcagatcc aaagttgctc cccccgcgtc tcttgttggc
     3601 gatgaccgca atggacccgc ccccgccagc aggggaattt acgcccccga tggaggccgc
     3661 gatcgccgaa gagcgggtgt tagtagccct cggtaagggc attgactatt tgagccagta
     3721 tgccacacca gagcaagttc agcgtacctg ttacgccacc ttctttatgt tcgtctcttg
     3781 gagtgcctac gcagcgtggc gttacactgg cgagtatccg ccagcgtgga agtatctggc
     3841 tgcgcgacag cacgacagct tctacacctc catgacacta atcgacccca tcggaggcta
     3901 cgtcctccca ccagatcttt tcttcgatcc gcgcgtccgt cacacagcct tcctagctgg
     3961 gacggcggtc gttctggtca acgatctcct ttcggttgcc aaagatctgg cagacgagca
     4021 gccacctgtc aacatggtgc tacagattgc ggcggatcgg ggctgctcta tcgaagaggc
     4081 gacggagatc actgtcaagt ttcataacga cttggttcat gactttcggg aacgacacca
     4141 gaagctccaa gctgtgccca acgttgagct tcagaggttc ctgcgcggat tgcgcggctg
     4201 gatgggcggc gcatttgaat ggcataacag caacccgcgc tataaaaaca gcaacggtgc
     4261 ttcacagcca gattcacaat cactagccta attataggag cgcacatagt tcaaccagat
     4321 ttcatgcggt tgaataaacc agagtatgtg tatgtatata gaaagcgagg taaaaaatga
     4381 cccaagactt taactcccat gggctggcgg agcagtcgag cctgagtaca gatgcggcgc
     4441 ggaagttagc cacaactacc aagactactc cgcaaactca gggtattagt tcgcgctgga
     4501 tcagccgcct gctgccttgg gtggagctac gcggcggctc ctatcgggtc aaccgacgtt
     4561 tgacctatgc gctgggcgat ggacgagtga cctttaccaa cgtgggggct accatacgag
     4621 tgatcccgca agagttgcgc gaaatagccc tgttgcgcga ctttgacgag gaggcagtgc
     4681 taaacaggtt ggcagagcgt tttgtgcagc aggagtttca acccggtgat gtgttggtgg
     4741 aaaagggaac ccccgccgac cagattgtgc tgattgccca cggcaaggtc agtaagatcg
     4801 gtacggggaa gtatggggat gacatggagc ttacggtatt gaccgatgga gaccacttca
     4861 cctaccaggc tattatcgaa tccgccgatc tctgggactt caccatgaag gcggttacac
     4921 gctgcacggt cttggtgcta acacagcaat cgtttgaaga tgcagtggct tcctctgaat
     4981 cactgcaagc ccacatcgaa ggatttaagg ctcgcccgcc aaaagcccaa gatagatatg
     5041 gtcagtccgc gattgcgtta gctgctggtc atacaggcga acccgtgctg cccacgactt
     5101 ttgttgatta tgagacctcg ccgcgcgaat acgagttgag cctagtgcag acggtgctgc
     5161 gtgtccatac gcgcgttgcc gatctctaca acgatccaat gaaccaaatc gaacaacagc
     5221 tcaaattgac tgtgcatgag ttgcgcgagc gtcaggagta cgaattggtc aataatcaag
     5281 agtttggtct gctgcacaac gccgacctca gccaacgctt ccccacccgc aacggtctac
     5341 ctcacccaga tgattttgat gagctgatca cccgccgccg caattcacag tatatttttg
     5401 cccacccacg tgccattgct gcctttgcgc gtcagtgcaa caagatgggg attgatttgg
     5461 aaagtatcga tgtaggtgga aaccgaattc cagcttggcg cggcattcct atctatcctt
     5521 gtggcaaaat ccccattgac agaaagactc aaactacatc gattatcgtt atgcgtactg
     5581 gtgaggacaa tcagggtgtg gtaggtcttc agcctgcgga gttacctgat caatatgagc
     5641 cagggttgaa cgtgcgcttt atgggcatta acgaaaaggc catcatctcc tatctagtca
     5701 gcaactactt cgccgccgct atccttgtcc ccgatgctgt cggcattctc gaggatgtcg
     5761 agatcgggcg gtaggacaga acgaatgtgt tccattgagg ttagatcgcc ctagaaagtg
     5821 tggcttagct cgaatattta atcggtgcgg agaaattgag agatttgaac agtattgttg
     5881 tggttgaatc acagcgcaca aaaaatggag ttaattccaa agaatatcgc tactatctac
     5941 tcactacttt a
//
