LOCUS       BGC0000693             29033 bp    DNA     linear   BCT 26-JUL-2016
DEFINITION  Bacillus circulans butirosin-biosynthetic gene cluster (orf1, btrR1,
            btrU, btrR2, btrT, btrS, btrN, btrM, btrL, btrA, btrB, btrC, btrD,
            btrE, btrF, btrG, btrH, btrI, btrJ, btrK, btrO, btrP, btrV, btrQ,
            btrW, btrX), complete and partial cds.
ACCESSION   BGC0000693
VERSION     BGC0000693
KEYWORDS    .
SOURCE      Niallia circulans
  ORGANISM  Niallia circulans
            Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Niallia.
COMMENT     On Jul 11, 2005 this sequence version replaced AB066276.1.
            ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-07 20:17:54
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 29033
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     gene            complement(<1..514)
                     /gene="orf1"
     CDS             complement(<1..514)
                     /codon_start=1
                     /function="unknown"
                     /gene="orf1"
                     /product="hypothetical protein"
                     /protein_id="BAE07056.1"
                     /transl_table=11
                     /translation="MELHSIKWLTRREFTPHMPTTQALSNFLREQPQNKIGAVAAFIES
                     HVRENWQEILVKENDRLLKAYAEAGDPAYGTYLNLLFLPVHRQLNEAGLRPEPRLPGNF
                     DISREWGRNPDQSDQERWMWSTLHPTEGPPAGTIVTITYHDHTRFRIPRRPGIIALTET
                     RQQDIVEA"
     source          1..29033
                     /db_xref="taxon:1397"
                     /mol_type="genomic DNA"
                     /organism="Niallia circulans"
     subregion       1..29033
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000693"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..29033
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="amglyccycl"
                     /region_number="1"
                     /rules="((DOIS or DHQ_synthase or ValA or salQ) or strH or
                     strK1 or strK2 or NeoL or SpcFG or SpcDK_glyc)"
                     /subregion_numbers="1"
                     /tool="antismash"
     protocluster    1..25397
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[7410:15397]"
                     /cutoff="20000"
                     /detection_rule="((DOIS or DHQ_synthase or ValA or salQ) or
                     strH or strK1 or strK2 or NeoL or SpcFG or SpcDK_glyc)"
                     /neighbourhood="10000"
                     /product="amglyccycl"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      7411..15397
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="((DOIS or DHQ_synthase or ValA or salQ) or
                     strH or strK1 or strK2 or NeoL or SpcFG or SpcDK_glyc)"
                     /neighbourhood="10000"
                     /product="amglyccycl"
                     /protocluster_number="1"
     cand_cluster    1..25397
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((DOIS or DHQ_synthase or ValA or salQ)
                     or strH or strK1 or strK2 or NeoL or SpcFG or SpcDK_glyc)"
                     /kind="single"
                     /product="amglyccycl"
                     /protoclusters="1"
                     /tool="antismash"
     gene            878..1531
                     /gene="btrR1"
     CDS             878..1531
                     /codon_start=1
                     /function="butirosin biosynthesis"
                     /gene="btrR1"
                     /gene_functions="regulatory (smcogs) SMCOG1057:TetR family
                     transcriptional regulator (Score: 179.7; E-value: 1.3e-54)"
                     /gene_kind="regulatory"
                     /product="possible transcriptional regulator"
                     /protein_id="BAE07057.1"
                     /transl_table=11
                     /translation="MSPRTKEQNEAIRNQRKREVLQAAVQVYVEKGYAAAEMGDIADRA
                     GLAHGLVYYYFKNKKALFRELYETMMEESKCFTKSYFEQKGSVSDLFESYARVVCERVR
                     VNPAIPRFYMRISLDVHHLYTPEEFSPFSWVKSFLQEMTQAIEKGIGEGTIRPGNPDLM
                     ARQFWGAVSQGMNYLDQAQQEWMANGMPETEKKEQWEKALTQVVESSVSILRLR"
     gene            1620..2195
                     /gene="btrU"
     CDS             1620..2195
                     /codon_start=1
                     /function="butirosin biosynthesis"
                     /gene="btrU"
                     /product="possible oxidoreductase"
                     /protein_id="BAE07058.1"
                     /transl_table=11
                     /translation="MAHPNLQQSRVNKRWVEELKKHPGEVTVHELYGAYPEKVLDIGRE
                     QALLAECERLVLQFPLQWYSTPPLLKQWLDEVFTTGWLFGPGGKAVAGKELLIAVSIGG
                     AAETYEAGGLIGYTISELTRPLQAFANQIGMTMLPHFKFHGANQAADELIESSAERYVR
                     HILTPELDPRIARRRMLNDMKQKMQASL"
     gene            2237..3379
                     /gene="btrR2"
     CDS             2237..3379
                     /codon_start=1
                     /function="butirosin biosynthesis"
                     /gene="btrR2"
                     /product="possible transcriptional regulator"
                     /protein_id="BAE07059.1"
                     /transl_table=11
                     /translation="MTFGFRESIMGMVGTKQTYGENLEESDMSTEPNTAGWDAIDKEFV
                     KLYGDQEPKHYGTLISYALGGPDPLDGISAYKTDEPAPHWHFVTFGFSELYDKESDHPE
                     ESGYGFELTFRLDRGAEEEEPPAWAINLLQNMGRYVFNSGNIFRSGDYMDANGPICLGS
                     DTRLTALAFIRDPELPAIDTPNGRVEFIQMVGITRDELEAMQTWNTLGMLSACLPYMPH
                     YITNLSRDSLLKVEAVSEAAEKGMREDGSNTGFLFVDQLAWEPGKKGWLSKAPAALTLG
                     AKQAGIIGKLLQGRILKGKDFTLVGPEVRAIFKAGEKPECAAGDGEVRIMLDEQAAGEL
                     SRGLIPKESVLEIPSLQGTVIRIVKTNIKDSEGNVVEVIG"
     gene            3646..3879
                     /gene="btrT"
     CDS             3646..3879
                     /codon_start=1
                     /function="butirosin biosynthesis"
                     /gene="btrT"
                     /product="BtrT"
                     /protein_id="BAE07060.1"
                     /transl_table=11
                     /translation="MQAIGSTLSEMNLCSGVLRIKTLHIMRGTIRANFLPFIVIKCRLG
                     YRQAIGTKEAEENDPVVSSVLFVVTQALFPAK"
     gene            4126..5382
                     /gene="btrS"
     CDS             4126..5382
                     /NRPS_PKS="Domain: Aminotran_1_2 (22-170). E-value:
                     2.3e-10. Score: 31.9. Matches aSDomain:
                     nrpspksdomains_btrS_Aminotran_1_2.1"
                     /codon_start=1
                     /function="butirosin biosynthesis"
                     /gene="btrS"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DegT_DnrJ_EryC1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1056:DegT/DnrJ/EryC1/StrS aminotransferase (Score:
                     392.2; E-value: 4.2e-119)"
                     /gene_kind="biosynthetic-additional"
                     /product="L-Glutamine:2-deoxy-scyllo-inosose
                     aminotransferase"
                     /protein_id="BAE07061.1"
                     /sec_met_domain="DegT_DnrJ_EryC1 (E-value: 2.4e-107,
                     bitscore: 350.0, seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTIPFDHWPEWPQHSDRTRRKIEEVFQSNRWAISGYWTGEESMER
                     KFAKAFADFNGVPYCVPTTSGSTALMLALEALGIGEGDEVIVPSLTWIATATAVLNVNA
                     LPVFVDVEADTYCIDPQLIKSAITDKTKAIIPVHLFGSMANMDEINEIAQEHNLFVIED
                     CAQSHGSVWNNQRAGTIGDIGAFSCQQGKVLTAGEGGIIVTKNPRLFELIQQLRADSRV
                     YCDDSSELMHGDMQLVKKGDIQGSNYCLSEFQSAILLDQLQELDDKNAIREKNAMFLND
                     ALSKIDGIKVMKRPPQVSRQTYYGYVFRFDPVKFGGLNADQFCEILREKLNMGTFYLHP
                     PYLPVHKNPLFCPWTKNRYLKSVRKTEAYWRGLHYPVSERASGQSIVIHHAILLAEPSH
                     LSLLVDAVAELARKFCVTH"
     aSDomain        4192..4635
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_btrS_Aminotran_1_2.1"
                     /evalue="2.30E-10"
                     /label="btrS_Aminotran_1_2.1"
                     /locus_tag="btrS"
                     /protein_end="170"
                     /protein_start="22"
                     /score="31.9"
                     /tool="antismash"
                     /translation="EEVFQSNRWAISGYWTGEESMERKFAKAFADFNGVPYCVPTTSGS
                     TALMLALEALGIGEGDEVIVPSLTWIATATAVLNVNALPVFVDVEADTYCIDPQLIKSA
                     ITDKTKAIIPVHLFGSMANMDEINEIAQEHNLFVIEDCAQSHGS"
     gene            5412..6164
                     /gene="btrN"
     CDS             5412..6164
                     /codon_start=1
                     /function="butirosin biosynthesis"
                     /gene="btrN"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR04085"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) SPASM"
                     /gene_kind="biosynthetic-additional"
                     /product="BtrN"
                     /protein_id="BAE07062.1"
                     /sec_met_domain="TIGR04085 (E-value: 2.5e-09, bitscore:
                     28.3, seeds: 281, tool: rule-based-clusters)"
                     /sec_met_domain="SPASM (E-value: 1.9e-17, bitscore: 54.4,
                     seeds: 166, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKLFSMIEVEVNSQCNRTCWYCPNSVSKRKETGEMDPALYKTLM
                     EQLSSLDFAGRISFHFYGEPLLCKNLDLFVGMTTEYIPRARPIIYTNGDFLTEKRLQTL
                     TELGIQKFIVTQHAGAKHKFRGVYDQLAGADKEKVVYLDHSDLVLSNRGGILDNIPQAS
                     KANMSCMVPSNLAVVTVLGNVLPCFEDFNQKMVMGNIGEQHISDIWHNDKFTSFRKMLK
                     EGHRGKSDLCKNCNNVSVQTEEQYDYVL"
     gene            6252..7421
                     /gene="btrM"
     CDS             6252..7421
                     /codon_start=1
                     /function="butirosin biosynthesis"
                     /gene="btrM"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045:glycosyl transferase group 1 (Score: 187.4;
                     E-value: 7.9e-57)"
                     /gene_kind="biosynthetic-additional"
                     /product="glycosyltransferase"
                     /protein_id="BAE07063.1"
                     /sec_met_domain="Glycos_transf_1 (E-value: 6.7e-40,
                     bitscore: 127.3, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGGMQVQITNLTEWLADQGVRQDVLTTGIPGIPRTLQLRDRLTVH
                     SVRFMTLPFKSSQTGTVFLDQSWFMGAVKWIVLNGKKNNYDAIHVHASGVVWPLLAGMF
                     AQKYLKKPLILTIHCSRIFTYKPMNKWDQFVHDFVKSVELKSIKLSHKAVVLTDKRLDS
                     YNRLLEDSSKMTAISDCIGSNHLSHSIDCPFCSRLKTELLGKKTVLFLGRIAHEKGWST
                     FVSVAKELADKIGDLQFIVCGDGPQREAMEEQIKAANLQNQFRITGFISHKFVSCYLHH
                     AQLFLLPSHHEEFGGSLIEAAIAGVPIISTNNGGPADIFTHGETAILKDPGDVSGIADE
                     AYKILTNDSVAESLRLHSRPEVVSKFLPHCVYPNYLNLYSSKEAAVHEG"
     gene            7411..9225
                     /gene="btrL"
     CDS             7411..9225
                     /codon_start=1
                     /function="butirosin biosynthesis"
                     /gene="btrL"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     amglyccycl: NeoL"
                     /gene_kind="biosynthetic"
                     /product="BtrL"
                     /protein_id="BAE07064.1"
                     /sec_met_domain="NeoL (E-value: 3.7e-171, bitscore: 560.2,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKDNPISLFHELESYVRGSKDTLHRYMYACILEQRLADLLLLSRC
                     GVHPDDEDRLAVRLASQRSLCVQAASDLAEGTEEARGSTPAWPDCKVTKSSAAQKRPEL
                     LSLVKVMQELRTPECSGYDLCDLEVCREDALHWLRDHADPSSPPVLIGVRTGGAFYAPL
                     WSSALQQRWGNKALYHTVRALRMPSDPGASLYLPEELSILPAAIEPDTDIVILEDQPHT
                     GGTVLELAGRLSAKYRLNKPVWVSSPGRLFQIENGRLAKCSDRIPLNTGTRKRIWQMLG
                     NSEEVAEFLLPKLGLTDAHPADLEAVPYKSVPQWNDPMYRREVPFRINPKKTPFYIRRK
                     STNEPVVFAKFIGKDLFGDFQFHQLKKFEKYFPDILAYQDGYVMTKYEPGLKEMREMFV
                     NMPSAVTREICQSVSGYWKTLLDSCQISGGHPVHPLADHWQARLGEMEDFIGRKLPYDL
                     DWFERSLHTEWTSSAHVYTSLPYANQYGHWKARLSAGQRLKTYRFHIDSTWGGTSSIEV
                     ELASFLLENRVRPDDFRLLVNQVKKEAGSLTIEAVTDALPIACVLQAANLLKQAKDESR
                     LSKELILSEAMQSFEYLQAMKPKLSDVR"
     gene            9272..12949
                     /gene="btrA"
     CDS             9272..12949
                     /codon_start=1
                     /function="butirosin biosynthesis"
                     /gene="btrA"
                     /product="BtrA"
                     /protein_id="BAE07065.1"
                     /transl_table=11
                     /translation="MNSFGIKCERLLELRNNETFTAHIPPLYPIDPKPAVEFLEQYSGT
                     EEELPGRLARYLEQREPSRFDQIKKVCGDLPWIIRSSGEEDLNETTNAGAYLSLVCEHR
                     DNFYEVLAQVMLSGSTEHARNQNRRFVSRDLHDHEPIPVFVQPLVQSEHPGHPVPYDAT
                     PYVRKEDLQTIIGLLKNLHSTMPDYALDCEWVGDSDFGTISMTSLTELADGKLTGQIAF
                     GFDLFSTQHVKANNSVRYLLPGDELNLWQGHIFQEVNMQSLFLVQTRPAKNYRLFQHVY
                     ELSASSKKELEKDAALCLDAKEFISQGGKPFFGKFIKAVTLNEAWNAYLQYTGKDVKED
                     IAAILVEFGTRTEHAGIMFNQVGIPVIRIEIALIPESMTYIVLDPFSLQCRLYSHNKEV
                     KALEYETREIIPLPDNCILVQDREGKHQQREAMAVSADEYFNEVLNKPFFSEKTKEHLT
                     ANSAYPAYSFIFHGKDDARSPSYYANDAERLTAETVDDFLKSREWPEASANYFLALFHA
                     KNLLKDGNIAAYVGRQPDDRGRSNETLVYWKVLRIHHLLAKCSSRDRDTLLERVRAEMS
                     VNSAVIELRLDVLFLFEQYLDALCIYSKREEEALIQSFIGILSSVKDEDLPVVLDLARN
                     GIFNAGHLCQFLKHAIENRHLFDLYRNYSLVGKEFAIIPYDLSVVSRFPYFNEVFVDLN
                     NEMNKYESLGELSSSIFHTVVETYDKSAKEILLSVIDDGSPSYYQAYLRLLTEWLGLIR
                     QFDKNEKVIQTFLDWIGVQEDRQAEIEDYFLEEIHWKTRIEEAEAGTAEDLSLTNLHQL
                     HNVLHQWSLHLVPASNGAHMPLFVKEMVAFAQSFSEQENRLLRMQKDFFEIELAMCTHK
                     AGFMFYKHSVTVEFSEPPSVHDDEIARLSAFASFMNKCNDWFEAYRFRTHYEKVAGTWT
                     CYMTVKSVEDGALQQSDYKTIFHIIRFVLDSSYDFSHNPLSAVEDLSAQLNEPEWKDIF
                     RKLVNYRVHFDDEGQFINLKLFAMSTFFTYLCLSPSLRSRLLSLYRDGFDRMVGELAVL
                     EDGMARAGHYSEWLEHYESATYTALFLAAIYPLETVGKLESLRDRRLTYDIVARNLLKH
                     KDAANKVIETLPTWDKALQSSMTDKLLHYCPAKFFKTVSSVNEVAKELSKRSKSFKRAK
                     QVLLNAYADSLEPDLLRQFIDDLQYVPYAGTEAQNQQLDKYLTDSGKKRYDIQKEILYI
                     "
     gene            12990..14288
                     /gene="btrB"
     CDS             12990..14288
                     /NRPS_PKS="Domain: Aminotran_3 (34-314). E-value: 1e-14.
                     Score: 46.1. Matches aSDomain:
                     nrpspksdomains_btrB_Aminotran_3.1"
                     /codon_start=1
                     /function="butirosin biosynthesis"
                     /gene="btrB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013:aminotransferase class-III (Score: 225.7;
                     E-value: 1.6e-68)"
                     /gene_kind="biosynthetic-additional"
                     /product="possible aminotransferase"
                     /protein_id="BAE07066.1"
                     /sec_met_domain="Aminotran_3 (E-value: 4.9e-16, bitscore:
                     49.1, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKQETVKSSEQLLSVLGTYIDSPVDPFRKERVMFSRGSGAYLFDY
                     DGGNYIDLMNGKGSIILGHNDPSVNAALRNFLEQDREVVTGPSKPIIDLAERIKKDSAL
                     PDAKVSFYTTGTAACRAAVYAARDYSGKKIVLSSGYHGWDPMWRQQGPLLEPNEDGVIE
                     FYFIPELLERALTAHKDQVALVIFSPDYTYLSASTMERILGICRAHGVLVCCDDVKQGY
                     RHRQGSSLELVTTEKADMYVFSKGLSNGHRISCVVSSDEIMAETKEHTYTAYYQMLPIL
                     SSLETLKKMESGKGYDLIRSYGQTLTGNLKELFVQSSLPIEVNGSSIFQLVFGDEELEE
                     AFYREAFIQGLILFEGDNQSLSLCMDKDVQVDLIRRFANVTDVLSEQFKHLRGKEVTTE
                     QTFRTAWNMIDGASDLLPYEKQLKLLDNLIGGG"
     aSDomain        13092..13931
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_btrB_Aminotran_3.1"
                     /evalue="1.00E-14"
                     /label="btrB_Aminotran_3.1"
                     /locus_tag="btrB"
                     /protein_end="314"
                     /protein_start="34"
                     /score="46.1"
                     /tool="antismash"
                     /translation="SRGSGAYLFDYDGGNYIDLMNGKGSIILGHNDPSVNAALRNFLEQ
                     DREVVTGPSKPIIDLAERIKKDSALPDAKVSFYTTGTAACRAAVYAARDYSGKKIVLSS
                     GYHGWDPMWRQQGPLLEPNEDGVIEFYFIPELLERALTAHKDQVALVIFSPDYTYLSAS
                     TMERILGICRAHGVLVCCDDVKQGYRHRQGSSLELVTTEKADMYVFSKGLSNGHRISCV
                     VSSDEIMAETKEHTYTAYYQMLPILSSLETLKKMESGKGYDLIRSYGQTLTGNLKELF"
     gene            14291..15397
                     /gene="btrC"
     CDS             14291..15397
                     /codon_start=1
                     /function="butirosin biosynthesis"
                     /gene="btrC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     amglyccycl: DHQ_synthase"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     amglyccycl: DOIS"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1183:3-dehydroquinate synthase (Score: 303.1; E-value:
                     3.7e-92)"
                     /gene_kind="biosynthetic"
                     /product="2-deoxy-scyllo-inosose synthase"
                     /protein_id="BAE07067.1"
                     /sec_met_domain="DOIS (E-value: 2.3e-153, bitscore: 501.0,
                     seeds: 17, tool: rule-based-clusters)"
                     /sec_met_domain="DHQ_synthase (E-value: 1e-68, bitscore:
                     222.1, seeds: 76, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTKQICFADRCFNFAFGEHVLESVESYIPRDEFDQYIMISDSGV
                     PDSIVHYAAEYFGKLAPVHILRFQGGEEYKTLSTVTNLQERAIALGANRRTAIVAVGGG
                     LTGNVAGVAAGMMFRGIALIHVPTTFLAASDSVLSIKQAVNLTSGKNLVGFYYPPRFVF
                     ADTRILSESPPRQVKAGMCELVKNMLILENDNKEFTEDDLNSANVYSPKQLETFINFCI
                     SAKMSVLSEDIYEKKKGLIFEYGHTIGHAIELAEQGGITHGEAIAVGMIYAAKIANRMN
                     LMPEHDVSAHYWLLNKIGALQDIPLKSDPDSIFHYLIHDNKRGYIKLDEDNLGMILLSG
                     VGKPAMYNQTLLTPVRKTLIKEVIREGL"
     gene            15400..16227
                     /gene="btrD"
     CDS             15400..16227
                     /codon_start=1
                     /function="butirosin biosynthesis"
                     /gene="btrD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PIG-L"
                     /gene_kind="biosynthetic-additional"
                     /product="BtrD"
                     /protein_id="BAE07068.1"
                     /sec_met_domain="PIG-L (E-value: 3.1e-21, bitscore: 67.2,
                     seeds: 137, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNQDKRAFMFISPHFDDVILSCASTLMELMNQGHTCKVLTVFGGC
                     PSVRFQPGEIARQYAAEDLGLFEDEIEGDHLSILVARRLQEDQQAFRHLPGVQVEVLSF
                     PDAIYRENKGQPYYRTEADLFGIPDKQDEDIFLPKIETYLQSCDLARKYTWVFPAISKH
                     VDHRLLTKAGLRLMSQGYPVLFYSEFPYWQQHNEFLQDGWRQLELRNSVYTPVKRAAVL
                     EYKTQLLGLFGEEAETKINNGGVLSEAELFWIQETDTQAWRVFRSLSPEPLQT"
     gene            16251..17300
                     /gene="btrE"
     CDS             16251..17300
                     /NRPS_PKS="Domain: PKS_ER (17-236). E-value: 2.9e-18.
                     Score: 57.8. Matches aSDomain:
                     nrpspksdomains_btrE_PKS_ER.1"
                     /codon_start=1
                     /function="butirosin biosynthesis"
                     /gene="btrE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1040:alcohol dehydrogenase (Score: 124; E-value:
                     1e-37)"
                     /gene_kind="biosynthetic-additional"
                     /product="possible oxidoreductase"
                     /protein_id="BAE07069.1"
                     /sec_met_domain="ADH_zinc_N (E-value: 2.3e-26, bitscore:
                     83.1, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEILTYTGPSRLIVETAEELPLKSGECRIQSLYSGISHGTEMGAY
                     RGIAPYFTRDMDSETRLFEDLAEEHKVKYPIRSCEDDAWFIGYSNVGKIIEVGAEVEGF
                     TVGDIVFSHGRHQTIVCKHHKKIFKLPQKLEPELGIFYTNLMTAYNAVLDTRIKIGDVV
                     VVSGLGVVGQLIAQLAKLSGATVVGVDPLENRLNIAREVEIDYVFNPNRTDVAKEIREI
                     TNNRGADAVFEVSGHSTALNQAIRIAAPDTVITAVGWYQGGQSVLNLSEEFHQNRITIR
                     ASQTLGIDPSISHMYDDARRRNIGKDLLLKLKLRNLISHRIPFGDAPKAYEMIDKHPHE
                     VLQVVLTYS"
     aSDomain        16302..16958
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_btrE_PKS_ER.1"
                     /evalue="2.90E-18"
                     /label="btrE_PKS_ER.1"
                     /locus_tag="btrE"
                     /protein_end="236"
                     /protein_start="17"
                     /score="57.8"
                     /tool="antismash"
                     /translation="EELPLKSGECRIQSLYSGISHGTEMGAYRGIAPYFTRDMDSETRL
                     FEDLAEEHKVKYPIRSCEDDAWFIGYSNVGKIIEVGAEVEGFTVGDIVFSHGRHQTIVC
                     KHHKKIFKLPQKLEPELGIFYTNLMTAYNAVLDTRIKIGDVVVVSGLGVVGQLIAQLAK
                     LSGATVVGVDPLENRLNIAREVEIDYVFNPNRTDVAKEIREITNNRGADAVFEVSG"
     gene            17312..18010
                     /gene="btrF"
     CDS             17312..18010
                     /codon_start=1
                     /function="butirosin biosynthesis"
                     /gene="btrF"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001:short-chain dehydrogenase/reductase SDR (Score:
                     153; E-value: 1.7e-46)"
                     /gene_kind="biosynthetic-additional"
                     /product="possible dehydrogenase"
                     /protein_id="BAE07070.1"
                     /transl_table=11
                     /translation="MIVFITGANRGLGLSLCEVFLERGHQVLAGVRNMNRAGQLLPLKT
                     KYKEELSIIPVDVSSQDSIRQAYSLITAVTPKLDAIINNAAREGIRCKKIQEVDIADFE
                     ETWKTNVLGPMAIIKSLKPMMESSSVKSILNISSEAGSISELPLKTYGYCISKAALNMF
                     TRMLERELKPENFKVVAVHPGWMKTEMGGEDAPYEPGEIARHLYERLTESPGKKADFNI
                     VDHLGNRMPP"
     gene            18025..18495
                     /gene="btrG"
     CDS             18025..18495
                     /codon_start=1
                     /function="butirosin biosynthesis"
                     /gene="btrG"
                     /product="BtrG"
                     /protein_id="BAE07071.1"
                     /transl_table=11
                     /translation="MISWTKAFTKPLKGRIFMPNLFVYGTLREGENNHKYMKEATLLSR
                     KASIAGSLVDTGNGYPGLLLENQLVAGEWYEVSEETLKRIDELEEYFGPGDTRNLFDRI
                     ECQVNESGGTHLGWTYVYNRDDYLETRFSDWKQYRLQHASGIEEKQDVPHSL"
     gene            18476..19384
                     /gene="btrH"
     CDS             18476..19384
                     /codon_start=1
                     /function="butirosin biosynthesis"
                     /gene="btrH"
                     /product="BtrH"
                     /protein_id="BAE07072.1"
                     /transl_table=11
                     /translation="MCLTRYDEKFFDCRKSQIIAYLDSQQVPVIPLFYNSYQSTAEIYR
                     QIFIENKSKWKYSEPSFSDDDLLRKGIRPVRASFPDFSQASDCLKDLLARHKLVFVWGD
                     EYCLPYRKEAFQAIHSTHSLVVTGYDGENKAYYVEDWDGLYGYLPAVHLEAAFDSLSRQ
                     MRTLLVLELNDEEMRENKQEDTDLFRKWLQAFEDDYIFYDRVLLDMRDYEENRLISMDH
                     GLRLIAASRHVFSKFLHYIDDAPEEVGLLIRNHQLANHIAAIVRRYIIAKQIDWDGAAC
                     KIRQLREQEDDFMRKLKSRYG"
     gene            19453..19716
                     /gene="btrI"
     CDS             19453..19716
                     /NRPS_PKS="Domain: PP-binding (12-79). E-value: 1.8e-16.
                     Score: 52.3. Matches aSDomain:
                     nrpspksdomains_btrI_PP-binding.1"
                     /codon_start=1
                     /function="butirosin biosynthesis"
                     /gene="btrI"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_kind="biosynthetic-additional"
                     /product="possible carrier protein"
                     /protein_id="BAE07073.1"
                     /sec_met_domain="PP-binding (E-value: 8.3e-17, bitscore:
                     52.3, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSLETQENVRTAQIVSCVNEILQTKEFITPETDLFLLGLDSVTAV
                     NLIVSLESHFGITFNDDELIVENFSTVRKITEHVTVKLEEAL"
     aSDomain        19489..19689
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_btrI_PP-binding.1"
                     /evalue="1.80E-16"
                     /label="btrI_PP-binding.1"
                     /locus_tag="btrI"
                     /protein_end="79"
                     /protein_start="12"
                     /score="52.3"
                     /tool="antismash"
                     /translation="QIVSCVNEILQTKEFITPETDLFLLGLDSVTAVNLIVSLESHFGI
                     TFNDDELIVENFSTVRKITEHV"
     gene            19713..20972
                     /gene="btrJ"
     CDS             19713..20972
                     /codon_start=1
                     /function="butirosin biosynthesis"
                     /gene="btrJ"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp_3"
                     /gene_kind="biosynthetic-additional"
                     /product="BtrJ"
                     /protein_id="BAE07074.1"
                     /sec_met_domain="ATP-grasp (E-value: 2e-09, bitscore: 28.0,
                     seeds: 20, tool: rule-based-clusters)"
                     /sec_met_domain="ATP-grasp_3 (E-value: 4.7e-13, bitscore:
                     40.3, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFTHPLDYYRLNGKQILWYMNIGEDQDSQASNYFPSVKDPQSEK
                     IVVQQEQQLLFLARPQDTVFFHTMPEQAFLDYWKERRLSLPSIICCDKLSQVPDLERYT
                     IIPFIVSDQLLELKRRYPHMDIIAPDLAVCREINHKFNTRRLMERNGFNVTTGYFCSDI
                     ESLEHAYEQLISAGFSKCVLKVPYGSSGKGLKVIDNERNFRFLLNYIQNRQTNVDLLLE
                     GWHPHRLSLTSQLFITEYEVHLLAVTEQIIDPNGVYKGTNFTPALSQSEAADYREEILR
                     AGELIRQMGYRGVLGIDSILDTNGELIPVIEINARLTQVTYILPLVIEQKKRYEFVESR
                     VLVFNSRADLDFEDYENDLSEVTRDLPVRIDLYNFCKASGAFKNTYKLFVLVSAHNSEQ
                     LIKARSLLDELNTKMTTAVH"
     gene            21000..22286
                     /gene="btrK"
     CDS             21000..22286
                     /codon_start=1
                     /function="butirosin biosynthesis"
                     /gene="btrK"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Orn_DAP_Arg_deC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Orn_Arg_deC_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1264:diaminopimelate decarboxylase (Score: 412.6;
                     E-value: 2.1e-125)"
                     /gene_kind="biosynthetic-additional"
                     /product="BtrK"
                     /protein_id="BAE07075.1"
                     /sec_met_domain="Orn_DAP_Arg_deC (E-value: 7.8e-22,
                     bitscore: 68.2, seeds: 500, tool: rule-based-clusters)"
                     /sec_met_domain="Orn_Arg_deC_N (E-value: 2.5e-45, bitscore:
                     145.5, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNLDQAEITALTKRFETPFYLYDGDFIEAHYRQLRSRTNPAIQFY
                     LSLKANNNIHLAKLFRQWGLGVEVASAGELALARHAGFSAENIIFSGPGKKRSELEIAV
                     QSGIYCIIAESVEELFYIEELAEKENKTARVAIRINPDKSFGSTAIKMGGVPRQFGMDE
                     SMLDAVMDAVRSLQFTKFIGIHVYTGTQNLNTDSIIESMKYTVDLGRNIYERYGIVCEC
                     INLGGGFGVPYFSHEKALDIGKITRTVSDYVQEARDTRFPQTTFIIESGRYLLAQAAVY
                     VTEVLYRKASKGEVFVIVDGGMHHHAASTFRGRSMRSNYPMEYIPVREDSGRRELEKVT
                     IAGPLCTPEDCLGKDVHVPALYPGDLVCVLNSGAYGLSFSPVHFLGHPTPIEILKRNGS
                     YELIRRKGTADDIVATQLQTESNLLFVDK"
     gene            22314..23339
                     /gene="btrO"
     CDS             22314..23339
                     /codon_start=1
                     /function="butirosin biosynthesis"
                     /gene="btrO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1083:oxidoreductase (Score: 54.1; E-value: 2.5e-16)"
                     /gene_kind="biosynthetic-additional"
                     /product="BtrO"
                     /protein_id="BAE07076.1"
                     /transl_table=11
                     /translation="MIALDTIQYYGQIPGVNHYNGKKEFMENAVKIAQLSDAYGIVGSL
                     SFFNHSVLDPWAVSSVIMRHTERHVPLIALQPYMYPPYTAAKLIQSFTYLYDRRIDLNM
                     ITGAVTGELQQTGGYIDHSSRYKKLHEYVQVLRLLLESDSAVSFKGDYYELNNLEFKPL
                     LPDKRLFPRIFMSGSSEEGLETGLKAADFVVTHPGPLEHFKRHFSEKVQGSAVQSAIRI
                     EIIARESAEQAWKIAHARYPGNRQGKIQLRMKTNSESSWQRMLAELALASETYDEVFWM
                     GGYMNGGIYSPVLVGDYEQVAAYLNEYYKLGVKAVLLGSMYSEEDFIHFSRVKEGISNP
                     V"
     gene            23383..24024
                     /gene="btrP"
     CDS             23383..24024
                     /codon_start=1
                     /function="butirosin biosynthesis"
                     /gene="btrP"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1101:phosphoglycerate mutase (Score: 102.8; E-value:
                     3.1e-31)"
                     /gene_kind="biosynthetic-additional"
                     /product="BtrP"
                     /protein_id="BAE07077.1"
                     /transl_table=11
                     /translation="MRLILIRHAQARCNILEDDALMDAYDPHCELTEAGIGQAVKLRDE
                     YPVSLTPSVIYSSPLKRARETAGIFRGRYPSVPFVEDERLSELKAPESFIPPITQGQWD
                     LYLEQRIRSPHLEIVKGLESLDVQRERIERFYKDLFRKYAEEACNIVIFTHAFSIQLSI
                     LFFLGLGNEQLLQWQIKASNTAMHIIHYDPTSGSFLLESLNNRSHLQTTG"
     gene            24041..24289
                     /gene="btrV"
     CDS             24041..24289
                     /codon_start=1
                     /function="butirosin binsynthesis"
                     /gene="btrV"
                     /product="BtrV"
                     /protein_id="BAE07078.1"
                     /transl_table=11
                     /translation="MDNKERNTLYQVVYAISGVTGEDGDELVETFKQGPMEVDKRDFFE
                     IVQRVESLFDCTLDMNLEGPYLIHADEIVTKITKLNV"
     gene            24471..25841
                     /gene="btrQ"
     CDS             24471..25841
                     /codon_start=1
                     /function="butirosin biosynthesis"
                     /gene="btrQ"
                     /product="possible dehydrogenase"
                     /protein_id="BAE07079.1"
                     /transl_table=11
                     /translation="MSHAEKAYARESDGTWGNNGYPWSASAVGGGTIFYAGISFRYKDV
                     DFDAREYVASDALDPQWPIGYSDLDPYYNKIEQWLGVSGDLNADPFKNSAGTGSLRTAH
                     SYSQQGALIDQAGKELGLHPFPTPLAVNTTDHNGYPGCDNLTSCTDYACPIGAKADSFT
                     RILKPVLKLEHVTLMKNTKALAFHQTDPSVIDSLECLDLDTKERFHIRAKQFVLAGNAI
                     QSAALVLRSTNKWWPDGVGNKSGLVGSGLSFKNSEYVSGWVDNHPYEQLNEPLKGLYST
                     VSITDYYQDPHCHSGLGGLIYEANPWTYYGHEGSGMYVQLECIIADQPMVTNKVRLSND
                     KEATGVPKIVLDYQTHEWDKRRLSFMIERAGEILTAMGAKRVQCNPSYYYLGSAHLHGT
                     CRAGTDERTSVVDKFGRFHTIDNLFAADGSFMPFAGGVNPTLTIQANALRIAENMSV"
     gene            25887..27644
                     /gene="btrW"
     CDS             25887..27644
                     /codon_start=1
                     /function="butirosin biosynthesis"
                     /gene="btrW"
                     /gene_functions="transport (smcogs) SMCOG1288:ABC
                     transporter related protein (Score: 387.6; E-value:
                     1.7e-117)"
                     /gene_kind="transport"
                     /product="possible ABC transporter"
                     /protein_id="BAE07080.1"
                     /transl_table=11
                     /translation="MIVIVSVLVRLGWFFREHKIRYTLAVLILVLVNGVEMLPPLLLGS
                     ALDHIQQNTLTPASLAQYIGFILGLSVVIYTLNYAWMFQLHGGANRLQLKMRSRLMAHL
                     LKMTPPFFEKYRTGDLMARATNDIKSIGETSGSGILTLVDSCLFSVTILITMGFFIDWN
                     LMLVTILPLPLIAAVITFFGKYLHRRFTIAQDAFGALNDQVLETVTGIRVVRAYVREEA
                     NQKNFETMAEDLFRKNVAIARVEALFEPTIRMLIGISYLIGLGYGTYLVFQQRISLGEL
                     ITFNVYLGMLIWPMYAIGELINIMQRGSASLDRLDEIMTYPPDVRDGEDPEEDIALPHT
                     LQFHRVSFRYPSSQTDNLKDITLHINRGQTIGIVGRTGSGKTTLLRQLLREYPTDRGEI
                     TVSGIPLERIRLEAWNRWIGYVPQEQLLFSKTVKENVRFGKENSTDGEVFRALELASFM
                     EDIHTLPDGIDTLVGERGVSLSGGQKQRIAFARAIISEPEIMLLDDALSAVDAKTEAGI
                     IANLIAERKGRTTLISTHRLSAVEHSDFIVVMDQGRIVQCGAHVQLVACEGWYKEQYES
                     QKLQMNLLK"
     gene            27659..>29033
                     /gene="btrX"
     CDS             27659..>29033
                     /codon_start=1
                     /function="butirosin biosynthesis"
                     /gene="btrX"
                     /gene_functions="transport (smcogs) SMCOG1288:ABC
                     transporter related protein (Score: 102.1; E-value:
                     5.1e-31)"
                     /gene_kind="transport"
                     /product="possible ABC transporter"
                     /protein_id="BAE07081.1"
                     /transl_table=11
                     /translation="MNVGTKLFKYVLYYKKSFIGALCLLLLMVAAELSGPIIAKRLIDQ
                     HILGIEKPWYETREPGENAVPYKGKHYKRGDYFHEQEEKGQEVRILQVQRQFVFVPEAV
                     KADGERIVQGDRLIIKRNAETGTYPADILSTRDLFLFYEPELEKIVNLILLYFGFVGAA
                     SLLEYGQTYALQVSSNRVIQKMRIDVLKQIHRIPISYFDRIPAGRIVSCVTNDTEAVKE
                     LFVTVLRNFFTGTVYITGILTALFILDVRLALICTLIIPALAGWICLYQKFASKYSQTI
                     RSTLSDINGLINETIQCMPVIRAFSRQRTVRQEFEDKNVRYFTYSNKLLTLKALTSNNL
                     VTVLRSLALAAIIWYFGSAGLSHQSVFTFGMIYAFVEYVERLFQPLVGMVNQLAQLNHS
                     LVSAERVFRLMEEEGSDVADEETPRMKGNVKFENVSFAYSDDHYVLRDISFEAKQGQTI
                     "
ORIGIN
        1 aggcctctac gatgtcctgc tgcctcgtct cggtaagcgc gataattccc ggacggcggg
       61 gaatacgaaa ccgggtatga tcatggtaag taatggtgac tatggttccg gcaggcggac
      121 cctcggttgg atgaagcgta ctccacatcc agcgctcctg atcgctttgg tccggattcc
      181 gcccccattc gcgggaaata tcgaaattac ccggcaggcg gggctccggc cgcagtccgg
      241 cttcatttag ctggcggtgt accggcagga agagcaggtt caagtacgtt ccgtaagccg
      301 gatctcccgc ttccgcgtaa gctttcagca ggcggtcgtt ctctttaacc agaatctcct
      361 gccagttctc gcgaacatga gattcaataa aggcggcgac ggccccgatc ttgttttgcg
      421 gctgttctct caagaaattg cttaatgctt gggttgtagg catatgaggc gtgaactccc
      481 ttcgcgttaa ccattttatg gaatgcagtt ccatcttaca acatgcatgc aaatgataac
      541 cttaccttcg aagatcaggc tgctgaagcc ggtttagaca tgaaacatta gcaagtgctt
      601 taagcgggta catgattaca tgcagcggat tcgaagcctt ttttaaggat caactgctgg
      661 aattcggtaa actgaccggt aaagaagaca aggcgaaaga atggggcgac cggtggtaga
      721 caaaaatcag cggtccgaag ccgcagatcc aagccgcctg aggcagcaga acggtgtcca
      781 tcctaaatcc gtaacctagg gcctggcggt tgacaacacc gttttatttt ttctataatg
      841 gcatggattt taaagacgga actaaaggtg aagcggcatg tctccacgta ctaaggaaca
      901 aaacgaagcg atccggaatc agcgcaaacg agaagtgctg caggcggctg ttcaggtcta
      961 tgtcgagaaa ggctatgcgg cggccgaaat gggggatata gcggatcggg cgggactcgc
     1021 tcacggtctc gtttattatt acttcaaaaa caagaaagca ttgttccgcg agctttacga
     1081 gaccatgatg gaagaatcca aatgctttac gaaatcttat tttgagcaaa aggggtccgt
     1141 gtcagacctg ttcgagagtt atgcccgggt cgtctgcgaa cgggtcaggg ttaatcccgc
     1201 gattccgcgt ttttacatgc ggattagttt ggatgtgcat catttgtata cgccggagga
     1261 attctctccg ttttcgtggg tgaaaagctt tttgcaggag atgactcagg ccattgaaaa
     1321 agggatcgga gaaggcacga ttcggccggg taatcccgat ctaatggcga gacaattttg
     1381 gggagccgtg tcgcaaggga tgaattactt ggatcaggcc cagcaggaat ggatggcaaa
     1441 cggcatgccg gagacggaaa agaaggaaca atgggagaaa gctttgactc aggtggtgga
     1501 gtcgtccgtt tccatcttaa ggctgcgcta aattttttta ttttaatatt gacttataac
     1561 tcaatcaaaa ataaaatgtg ggaaaggaga gtgatgtttg tctgaaaacg ttagtcgtcg
     1621 tggctcatcc gaatttgcag cagtcccgtg tcaacaaacg ttgggtggag gaattgaaaa
     1681 aacatcccgg cgaggttacc gtgcatgaac tgtacggcgc gtatccggag aaggtattgg
     1741 atatcgggcg ggagcaggct ttgctggcgg aatgtgaacg tctcgtgctg cagtttccgc
     1801 ttcaatggta cagcacaccg ccgcttctga agcagtggct cgatgaagtg ttcacaacgg
     1861 ggtggctgtt cggtccgggc ggtaaagccg tagccggaaa agaactcctg atcgccgttt
     1921 ctatcggggg agcggcggag acttacgagg cgggcggcct gatcgggtac acgatcagtg
     1981 aattgacccg ccctctgcaa gctttcgcca atcagatcgg catgacgatg cttccgcact
     2041 ttaaatttca cggtgcgaat caggcggcgg acgaactgat cgagagcagc gcggagcgat
     2101 acgtcaggca tatcttgact cccgagcttg atccccggat tgcccggcgg agaatgctta
     2161 atgacatgaa acagaagatg caggcctccc tgtaaaaaca aagccgatgc tccccacacg
     2221 gggaacttcg gcttatgtga cgttcggatt tcgggagagt ataatgggaa tggttggtac
     2281 caaacagaca tatggagaaa acttggagga atcggacatg agcacagaac cgaatacggc
     2341 gggctgggac gccattgaca aggaattcgt aaaactatac ggagatcagg aaccgaaaca
     2401 ttacggtacg cttatctcct atgcgctggg gggaccggat ccgctggatg gaatcagtgc
     2461 gtacaaaacg gacgagcctg ccccgcattg gcatttcgta acgttcggat tttcggaact
     2521 ttacgataaa gaatccgatc atccggaaga gagcggctac ggattcgaat tgacgtttcg
     2581 gctggaccgc ggtgcagaag aggaagagcc tccggcgtgg gcgatcaacc tgctgcaaaa
     2641 tatgggaaga tacgtattca acagcggcaa tattttccgg tcgggcgatt acatggacgc
     2701 caacggtccg atttgcctcg gttccgatac ccggctgacc gctctggctt tcatccggga
     2761 tccggagctt cccgcaatcg atacgcctaa cgggcgggtg gaatttatcc agatggtcgg
     2821 aattacgaga gatgagctgg aagccatgca aacctggaat acgcttggca tgctgtccgc
     2881 ctgtttgccg tatatgccgc actacataac gaatttatcg cgggattctc tgcttaaagt
     2941 cgaagccgtt tccgaagccg ctgagaaagg aatgcgggaa gacggctcca ataccggctt
     3001 tttgtttgtg gatcagctcg cctgggaacc gggtaaaaaa ggctggctga gcaaagctcc
     3061 cgcagcgctg acgctggggg ccaaacaagc cggcataatc ggcaagctgc tgcaaggtcg
     3121 gattttgaaa ggaaaagact tcactctcgt gggaccggaa gtccgcgcga tctttaaggc
     3181 gggagaaaag ccggagtgtg cggccggaga cggcgaggtc cggatcatgc tcgatgagca
     3241 ggctgccggc gagctgagca gaggcttaat ccctaaagaa agcgtgcttg agattccgtc
     3301 cttgcaggga acggtcattc gcatcgtgaa gacaaacatt aaggacagtg aaggtaacgt
     3361 agtggaagtt atcggctgac tgataacgat ctctgacggg tgcgattgca tacggacctt
     3421 tgaccgggag acggatggag aagatcggag agattctgat ttcgctgcaa atgcaggatt
     3481 aacggactat atcgcacgga gatcagggcc tggaaacggg cctttttttg tcgaaaagta
     3541 aaaataaaaa cggttcctta cgaagggtgg acactgtgcc catttgctta aggagccgtt
     3601 ttttgtgcgg ctgaaaaaac aggaaatgac gcaggacaca ggagcatgca ggcgatcggc
     3661 tctacattat ccgaaatgaa cctttgcagc ggggttttac ggattaagac cttgcatata
     3721 atgcgtggga ctataagagc taatttttta ccgtttattg tcataaaatg ccgattagga
     3781 taccggcaag cgataggtac gaaagaagca gaagagaatg acccggttgt aagttcagtc
     3841 ctattcgttg tgactcaagc cttatttcct gcaaaatgag caaccgaaac acggattgtg
     3901 cgtagaagca gaagaaaaga aaatccaaat ataggaaata accatatatt tatggtttat
     3961 atatcattca ttttagttct aaaacaaaac taaaaccatt taatttccaa aaatgggagt
     4021 tgacttttaa ttttggtaat tataggatgt aaatgtatcc ataaataaca cattatggaa
     4081 actatagtcg attaatagac tttaaatatc caaaggagag ttatcatgac cattccattt
     4141 gaccattggc cagaatggcc tcaacactcc gatcgtaccc gccgaaaaat tgaagaagtg
     4201 tttcagtcga acagatgggc gataagcgga tattggacgg gcgaggaaag catggaacgg
     4261 aaattcgcca aggcgtttgc ggatttcaac ggggtcccat actgtgtgcc gacgacgagc
     4321 ggctccacgg ctttaatgct tgcccttgaa gcgctcggaa taggcgaagg ggacgaagtc
     4381 attgttccca gcctcacttg gattgcaacc gctaccgccg tattaaacgt gaatgcgctt
     4441 cctgtttttg tggacgttga ggcggatacg tattgtattg atcctcaatt gataaagtcg
     4501 gccattacgg ataaaacgaa agccattatc ccggtccatt tgttcggaag tatggcgaat
     4561 atggatgaaa taaacgaaat cgcgcaagaa cacaaccttt ttgtgattga agactgcgcg
     4621 caaagccacg gttccgtatg gaacaaccag cgtgccggaa cgataggaga tataggggcg
     4681 ttcagctgtc agcagggcaa agtgttaacg gccggagaag gggggattat cgtgacgaaa
     4741 aatccccgtt tatttgaact catccaacaa ttgagagcgg attccagggt ttattgcgat
     4801 gattcctccg aattgatgca cggtgacatg cagctggtaa aaaaagggga cattcaaggc
     4861 tccaactatt gcttgtccga atttcaatcg gccatccttc tggatcaact tcaggagctg
     4921 gatgacaaga acgcgatccg ggaaaagaac gccatgttct tgaacgatgc cttaagcaaa
     4981 attgacggca ttaaggtgat gaagcgccct cctcaagtca gcagacaaac ttattacggc
     5041 tatgtattcc ggttcgatcc ggtgaaattc ggcgggttga atgcggatca gttctgtgaa
     5101 attcttcgcg aaaagctcaa tatggggacg ttttacctgc atccgccgta tctccccgta
     5161 cacaaaaatc cgttgttctg cccctggaca aagaaccgtt atttgaaatc ggttcgtaag
     5221 acagaagctt attggagagg gcttcattac ccggtctccg aacgtgcttc cgggcaatcc
     5281 atcgttatcc accatgctat tttactggct gaaccctcgc atctgtcgct tctggtcgat
     5341 gccgtagccg aacttgcccg gaaattttgc gtgactcatt aacgaaggat gattagcgag
     5401 gaggaatttg gatggataag ctgttttcga tgatcgaagt agaagtcaat tctcaatgca
     5461 accggacgtg ctggtactgc cccaatagcg tctccaagcg caaagagacc ggagagatgg
     5521 accccgccct gtataaaacg ctgatggaac agttaagttc gctggatttt gcgggacgta
     5581 tttcgtttca cttttacgga gagccgcttt tgtgcaaaaa tctcgatctg tttgtgggca
     5641 tgacaacgga atatatccct agagcaaggc ccattattta cacaaacggc gattttctta
     5701 ccgaaaaacg gttacagact ttgacggaac tgggcataca aaaattcatt gtgacccagc
     5761 atgccggagc caagcacaaa ttcagaggcg tatacgacca actggcggga gcggacaaag
     5821 aaaaagtggt ctatctcgat cattcggatc tggttttatc caatcggggc gggattctgg
     5881 ataatattcc tcaagcttcg aaagccaaca tgtcgtgcat ggtgccatcc aatctggccg
     5941 ttgttaccgt tttggggaac gtgctgccat gctttgaaga tttcaatcaa aagatggtta
     6001 tgggcaatat cggggaacag catatttcgg atatctggca caatgacaaa ttcacctcct
     6061 tccggaaaat gttaaaagaa gggcatcgag gcaaatcgga tctatgcaaa aattgcaaca
     6121 atgtaagcgt gcagacggaa gaacaatacg attatgtgct gtaacgggga gggggtgtac
     6181 gtttgaatat tttgcggctt acccctcatt actattttga gactaccgaa tggccgagcc
     6241 ggtttgatcc gatgggcggc atgcaggttc aaatcacgaa tttgacggaa tggctggccg
     6301 atcaaggagt ccggcaggac gtgctcacaa ccggcattcc gggcatcccc cgaaccttac
     6361 aacttcggga ccggttgacc gtccattccg tccgttttat gacgctgccc tttaagtcca
     6421 gccaaacggg aacggtgttt ctcgatcaga gctggttcat gggcgctgtc aaatggatcg
     6481 ttctgaacgg caaaaagaat aattacgatg cgattcatgt ccatgcaagc ggagtggtgt
     6541 ggccgttact cgcgggaatg ttcgctcaaa aatatttaaa aaagcctttg attctaacga
     6601 tacattgcag ccggatattt acgtacaagc ccatgaacaa gtgggatcaa ttcgttcacg
     6661 attttgttaa atccgtggaa ttaaagagca tcaaactcag ccataaagcc gtcgttctta
     6721 cggataagag actggacagc tacaaccggc tgcttgaaga ttcgtcgaaa atgacggcca
     6781 tcagcgactg tatcggatcg aatcatctca gtcattccat cgattgtcct ttttgcagcc
     6841 ggttaaaaac cgagcttctc ggcaaaaaaa cggttttgtt cctgggcaga atcgcacacg
     6901 agaaaggctg gtccaccttt gtaagcgttg cgaaagagct tgcggacaaa atcggcgatt
     6961 tgcaattcat cgtgtgcggg gacggtcccc agcgcgaggc aatggaagaa caaattaaag
     7021 cggcgaattt gcagaaccaa ttccgtatca ccggttttat ttcacataaa tttgtctcct
     7081 gctatctgca tcatgcccag ctgttcctgc tgccctccca ccatgaggaa ttcggcggaa
     7141 gcctgattga agcggccatt gcgggagttc cgatcatctc gaccaataac ggaggtcccg
     7201 ccgatatttt cactcacggt gaaaccgcga ttttaaaaga cccgggcgac gtgtcgggga
     7261 tcgctgacga ggcttataaa atattgacga acgactccgt cgccgagtcc ctgaggctcc
     7321 attccagacc cgaggttgtc agtaaatttt tgcctcactg cgtgtatccg aattatttga
     7381 atttgtactc ttccaaagag gctgccgtcc atgaaggata atccgatctc tttgtttcac
     7441 gaattggaaa gctacgtcag aggaagcaag gacactcttc atcgttacat gtacgcctgt
     7501 atcctggagc agcgattggc ggatttgctt ttgctgagcc ggtgcggcgt acatcctgat
     7561 gacgaggatc gtctggccgt ccggctggcc tcacaacgca gcctttgcgt acaggctgct
     7621 tccgaccttg cggaaggtac ggaggaggcg cgggggagta caccggcatg gcccgactgc
     7681 aaggttacca aatcgtccgc tgcgcagaaa agaccggagc tgctctcttt agtcaaagtg
     7741 atgcaggagc ttcgcacacc ggaatgctcc gggtatgatt tgtgcgacct ggaggtttgc
     7801 cgggaggatg ctctgcactg gctgagagac catgccgatc cgtcttcacc gcccgtgctc
     7861 atcggcgttc gaaccggagg cgctttctac gctccgttat ggtcttcggc tttgcagcag
     7921 aggtggggga ataaagccct atatcacacc gtcagagctc tgcggatgcc ctcggatccg
     7981 ggcgcgtcct tgtatttgcc cgaggaactt tcgatactgc cggcggcaat agagccggat
     8041 acggatatcg tgattctgga agaccagccg catacgggcg ggacagttct ggaactggcc
     8101 ggaagactta gcgcgaagta tcggctgaac aagcctgtct gggtttcttc tccgggccgg
     8161 ttgttccaaa tcgagaacgg ccggcttgcg aagtgcagcg atcgtatccc actgaatacg
     8221 gggacaagaa agcgcatctg gcagatgctc ggcaattcgg aagaagtggc ggagtttctt
     8281 ctgccgaagc tcggtttaac tgacgcgcat ccggcggatt tggaagcggt cccttataaa
     8341 tccgtgcctc aatggaatga tccgatgtat cgccgtgaag ttccctttcg gattaatccg
     8401 aagaaaactc cgttttacat ccgacgaaaa agcacgaatg agccggtcgt attcgcaaaa
     8461 tttatcggga aagatctttt cggcgatttt caatttcatc agctgaagaa gtttgaaaag
     8521 tatttccctg atattttagc gtaccaggac ggttatgtga tgaccaaata tgaaccggga
     8581 ttaaaggaaa tgagagaaat gttcgtcaac atgccttcag ccgtgacaag ggagatctgc
     8641 caaagcgttt ccggttactg gaaaacgctg cttgattcgt gccaaataag cggaggtcat
     8701 cccgttcatc ctttagccga tcattggcag gcccgtcttg gagaaatgga ggacttcatc
     8761 gggagaaagc tgccttatga tctggattgg ttcgaacgaa gtttgcatac cgaatggact
     8821 tcgtccgctc atgtttacac gtccttgcct tacgccaacc aatacggaca ctggaaagcg
     8881 aggctttcag ccggacaacg attaaaaacg taccgttttc acatcgattc cacctggggc
     8941 ggcacttcca gcatcgaggt ggagctggct tcgtttcttt tggaaaacag ggtgaggccc
     9001 gatgacttcc gtctgctggt aaaccaggta aaaaaagaag ccggctcctt aaccattgaa
     9061 gcggttaccg atgccctgcc gatcgcttgc gtcctgcaag ccgcgaatct attaaaacag
     9121 gcgaaggatg aaagccgctt gtcgaaagag ctgattttgt cggaagcgat gcaatcattc
     9181 gagtatcttc aggccatgaa gccgaagctt tcggatgtaa gataaatgtc acggtcgaac
     9241 gaaaccacgc acatgtttga ggaggctttg tatgaatagc ttcggaataa aatgcgagag
     9301 actgttggag ctgcggaaca atgaaacgtt tacagcgcat attccccctc tttatccgat
     9361 cgatcctaaa ccagccgttg agttcctgga acaatacagc ggtaccgagg aagaattgcc
     9421 gggtaggctg gcccggtacc tagagcagcg cgaaccatcc cgattcgatc aaattaaaaa
     9481 agtttgcggg gaccttccat ggattatcag aagctccggg gaagaagact taaacgaaac
     9541 gacgaatgcc ggcgcttatc tcagtttagt ttgtgagcac agggataact tttacgaggt
     9601 gcttgcgcag gtcatgctca gcggaagcac ggagcacgcc aggaaccaga atagacgttt
     9661 cgtgagccgg gatttgcacg atcatgagcc tattcccgtg tttgtccagc cgctggtcca
     9721 atcggagcat ccgggtcatc ccgttcctta tgacgcaacc ccttatgtca ggaaagagga
     9781 tttacaaacg atcatcggtc tgcttaaaaa cctccacagc accatgccgg actatgcgct
     9841 tgattgcgaa tgggtagggg actcggactt cggaacgatt tccatgacaa gcttaacgga
     9901 acttgccgac ggaaaactca cggggcagat tgctttcgga ttcgatcttt tctctacgca
     9961 gcatgtgaaa gcgaacaata gtgtccggta tctgctgccg ggcgacgagt tgaacttgtg
    10021 gcaggggcat atttttcagg aagtgaacat gcaaagtttg tttctcgtgc aaacaagacc
    10081 ggctaagaac taccgcttgt tccagcacgt ctacgagctg tcggcatctt ccaaaaaaga
    10141 attggaaaag gatgccgctc tttgtctgga tgcaaaggag ttcatctcgc agggcggcaa
    10201 gccgttcttc ggcaagttta ttaaggcggt gaccttgaac gaagcctgga acgcctatct
    10261 tcagtacacc ggcaaagacg ttaaggagga tatcgccgct atcctggtgg aattcggtac
    10321 gagaacggaa catgccggaa ttatgtttaa tcaggtcgga attccggtca ttcgtatcga
    10381 gattgcgctt ataccggaaa gtatgactta tatcgtactt gatccgtttt ccctgcagtg
    10441 ccgtttgtac agccacaaca aagaagtcaa ggcattggaa tacgagacca gggaaattat
    10501 tccccttccg gacaactgca ttcttgttca ggatcgtgag gggaagcacc agcagcggga
    10561 ggccatggcg gtatcggcgg acgagtactt taacgaggtg ttaaacaagc ccttcttctc
    10621 ggaaaagact aaagagcatt tgacagcaaa tagcgcctat ccggcttaca gcttcatttt
    10681 tcatggaaaa gatgatgccc gctcaccgag ttactacgca aatgacgccg aacggttgac
    10741 ggctgaaacg gttgacgatt ttttaaaaag ccgtgagtgg ccggaagcat cggcgaatta
    10801 tttcctggcc ttatttcatg cgaaaaactt gctcaaagac gggaacatcg ccgcttatgt
    10861 cggacggcag ccggatgacc ggggccggtc taatgaaacg ctcgtgtact ggaaggtgct
    10921 gcgcatccat catctgctgg ccaaatgcag cagccgggac cgggatacgc tgttggagcg
    10981 ggtccgtgcg gaaatgagcg ttaattcggc cgtcatcgag ctgcggctgg acgtgctgtt
    11041 tttattcgag caatatctgg acgccttgtg catttattcg aagcgggagg aagaggcgct
    11101 gatccaaagc tttatcggca tactttcttc cgttaaggat gaagacctgc cggtcgttct
    11161 ggatctagcc cgcaacggga tttttaacgc gggtcacctg tgccaatttt taaagcatgc
    11221 catagagaac cggcacttgt tcgatcttta ccggaactat tctctagtcg gcaaagagtt
    11281 tgccatcatt ccgtacgatt tgagcgtcgt cagcagattc ccgtatttta acgaggtttt
    11341 cgttgatttg aacaacgaga tgaataaata cgagtcgctc ggcgagttaa gcagctctat
    11401 ttttcatacc gtagtcgaaa cctacgataa atcggccaaa gaaattcttt tgagcgtgat
    11461 agatgacggc agtccgtctt attatcaagc gtatctcaga ctgttaaccg aatggctcgg
    11521 tttaatccgg cagttcgata agaacgagaa agtcattcaa actttccttg actggatcgg
    11581 ggtccaagaa gatcggcaag cggaaatcga agattacttc ctggaagaaa ttcattggaa
    11641 aacaagaata gaagaagccg aggcgggtac tgccgaagac ttgtcgttaa ctaatttgca
    11701 tcagctgcac aacgtgctgc accagtggtc gttgcacttg gttcccgcgt ccaatggcgc
    11761 tcatatgcct cttttcgtca aagagatggt ggcgttcgcc caatcttttt ccgaacagga
    11821 aaaccgattg ctgcggatgc aaaaagactt ttttgaaatc gagctggcga tgtgtacgca
    11881 caaggcaggg tttatgttct acaagcattc cgtaactgtc gaatttagcg agcctccttc
    11941 cgtacatgac gacgaaatcg cgagactatc ggcttttgcc tcttttatga acaagtgcaa
    12001 cgattggttc gaggcttatc gtttccgcac tcattacgag aaggtagccg gaacctggac
    12061 ctgttatatg accgtaaaat ccgtcgaaga cggcgcactt cagcaaagcg attacaaaac
    12121 gatttttcat atcataaggt ttgttttaga cagctcgtac gatttttcgc ataatccgct
    12181 ttctgcggtt gaggatttgt ccgctcaatt gaatgaaccg gaatggaaag atattttccg
    12241 gaagctggta aactatcgtg tccattttga cgatgaaggg cagtttatta acttgaaatt
    12301 gttcgccatg tccactttct ttacgtacct gtgtctttcg ccgtccctgc gaagccgcct
    12361 gctctccttg taccgggatg gattcgatcg tatggtcggt gagctcgccg tactggaaga
    12421 cgggatggcg cgcgccggtc attactcgga atggctcgag cattatgaat cggcaaccta
    12481 taccgcctta ttccttgcgg ctatttatcc tttggagaca gtgggcaagc tggagtcgct
    12541 gcgtgaccgg cggttgacct acgatatcgt ggccagaaac ttgctcaagc acaaagatgc
    12601 cgccaataaa gttatcgaga cgctgccaac ctgggataaa gcgctgcaat ccagtatgac
    12661 ggacaagctg ctgcactatt gtccggctaa atttttcaaa accgtttctt ctgttaacga
    12721 agtggccaag gagctctcca aacgaagcaa atcgtttaaa cgtgccaagc aggttttact
    12781 caacgcgtat gcggattcct tggagccaga ccttttgcga caattcatcg acgacttgca
    12841 gtatgtccct tatgccggga ccgaggcgca aaatcaacaa ctggataaat acctgacgga
    12901 ttccgggaaa aagagatatg acattcaaaa agaaattttg tatatctgat cgccgtatcg
    12961 cacgttacga ctaaggagtg aacgatgaaa tgaaacagga aacggttaaa agttcggaac
    13021 agctgctatc cgtactcggt acttatatcg attcgccggt cgatcccttc cgcaaggaaa
    13081 gagtgatgtt ttcaagggga agcggggctt atttgttcga ttacgacggc ggcaattata
    13141 tcgacctgat gaacgggaaa gggtctatta tactggggca caacgatccg agcgtcaatg
    13201 ccgccttgcg gaattttctg gagcaggacc gagaggttgt aacgggtcct tccaaaccga
    13261 tcattgatct ggccgaacgg atcaagaaag acagcgcttt gccggatgcg aaggtttcgt
    13321 tctatacgac cggaaccgcc gcctgcagag cggccgtgta tgccgcacgc gattacagcg
    13381 gcaagaaaat cgtcctcagc tcgggatacc acggctggga tcccatgtgg aggcagcagg
    13441 ggccactgct ggaaccgaat gaggatgggg tgatcgagtt ttatttcatc cccgagctgc
    13501 tggaacgcgc cttaacggcg cataaggatc aagtcgcctt ggtcatattt tcgccggact
    13561 atacgtattt atcggcttcg acgatggaga gaatccttgg catatgccgg gcgcacggag
    13621 tcctggtttg ctgcgacgat gtgaagcagg gctaccggca ccgtcaaggg tcttcccttg
    13681 agcttgtgac gactgagaag gccgacatgt acgtcttctc caaagggttg tcgaacgggc
    13741 accggatttc atgcgtggtg agttcggacg agatcatggc ggagaccaag gagcacacgt
    13801 acacggccta ctaccagatg ctgccgattc tgtcgtccct ggaaacgctg aaaaaaatgg
    13861 aatccggtaa aggctacgat ttgatacgtt cttacggtca aaccttgacg gggaatttga
    13921 aagagctgtt cgttcaatcc tctctgccca tcgaagtgaa cggcagctcc atttttcagc
    13981 tggtattcgg agacgaagag ctggaggagg cgttttaccg ggaagccttt attcagggac
    14041 tcattctatt cgagggggat aatcagtcgc tttccctgtg catggacaaa gacgttcaag
    14101 tggacctgat tcgcaggttt gcgaacgtaa ccgatgtctt atcggagcaa tttaaacacc
    14161 tgagaggaaa agaggttacg accgagcaaa cgttcagaac cgcttggaac atgattgacg
    14221 gggcatcgga tttgcttccc tatgagaaac agctcaaact tctcgataat ttgatcggtg
    14281 gaggttaaac atgacgacta aacaaatttg ttttgcggac cggtgtttta actttgcatt
    14341 cggcgaacat gttttggaat cggttgaatc ctatattccc cgggatgaat tcgatcaata
    14401 tatcatgatt tcggactcgg gggtaccgga ctcgattgtt cattatgcgg ccgaatactt
    14461 cggcaaactc gcccctgtac atattcttcg ctttcagggc ggagaagaat acaaaacact
    14521 ttcaaccgtg acaaatttgc aagagcgggc aattgctctg ggagccaacc gaagaaccgc
    14581 tatcgtagcg gttggcggag ggttaaccgg aaacgttgcc ggagtggcgg ccggcatgat
    14641 gtttcgcggg attgcgctta ttcacgttcc gaccacgttt ttggcggcct ccgattcggt
    14701 tctttcgatt aagcaggctg ttaatttaac gagcggaaag aacctggtcg gcttttatta
    14761 tccgccacgc ttcgtgttcg ccgatacccg aatcttgtcg gagtcgccgc cccgtcaggt
    14821 gaaagcggga atgtgcgagc tggtaaaaaa tatgctgatt ctggaaaacg acaacaagga
    14881 atttacagag gatgatttaa attcagccaa tgtgtattct ccgaagcagc tggagacgtt
    14941 tatcaacttc tgcatatcgg ccaaaatgtc ggtattaagc gaagatattt acgagaaaaa
    15001 gaagggcctg atctttgagt acggccatac gatcggtcat gcgatcgagc ttgccgagca
    15061 gggagggatc acgcacggag aagccattgc agtgggcatg atttacgccg ctaaaatagc
    15121 gaaccggatg aacctgatgc ccgaacatga cgtgtccgcc cattactggc ttttaaataa
    15181 aatcggggcc ttgcaggata ttccgctcaa atcggacccg gattcgatct tccattattt
    15241 aatccacgat aacaagaggg gctacattaa gctggatgag gataatttgg gtatgatttt
    15301 acttagcgga gtcggtaaac cggcgatgta taaccaaacg ctgcttacac cggtcagaaa
    15361 aacgctcata aaagaagtga tccgggaagg gctgtaagga tgaaccagga taagcgtgct
    15421 tttatgttca tctcgcctca cttcgatgat gtcattttgt cgtgcgcctc gactttaatg
    15481 gaactgatga atcaggggca cacctgcaag gtgctgaccg tattcggggg ctgcccgtcg
    15541 gttcgctttc agcccggcga gattgccaga cagtacgcag ccgaggattt gggattgttc
    15601 gaggatgaaa tagagggcga tcacttgtcc atactcgtgg cgcgccgact ccaggaggat
    15661 cagcaggcat tccggcatct tcccggtgta caggtcgaag tactttcctt tccggatgcc
    15721 atctacaggg aaaataaggg gcagccttat taccggacgg aggcagactt attcggcatt
    15781 ccggacaaac aggacgaaga tattttcctg cctaaaatcg aaacctattt gcagtcctgc
    15841 gatcttgccc ggaaatacac ctgggtattt cccgccatct ccaaacatgt cgatcaccgg
    15901 ctgctgacca aagccggttt gaggctgatg tcgcaagggt atccagtgct tttctattcc
    15961 gaatttccgt actggcagca gcacaatgag tttttacagg acggatggcg tcagttggaa
    16021 ttgagaaaca gcgtctacac accggtaaaa agagccgctg tattggaata caaaacgcag
    16081 ctgctcggat tgttcggcga ggaggcggaa acgaaaatca ataacggcgg agtccttagc
    16141 gaagccgagc tattttggat acaagaaacg gatactcaag cttggcgggt attccggtct
    16201 ttaagtcccg aaccgcttca aacctgacac aaagaaaagg agtgctttcc atggaaattt
    16261 taacgtacac gggtccaagc cgattaatcg tggaaacggc ggaagaattg ccgttaaaga
    16321 gcggcgaatg ccggatacag tccctctatt ccgggatcag tcacggaacg gaaatggggg
    16381 cttacagagg tatcgctccc tatttcacaa gagatatgga ttccgaaacc cggttgttcg
    16441 aggatctggc cgaagaacat aaagttaaat atccgatccg gagctgcgag gacgatgcct
    16501 ggtttatcgg gtattcgaac gtagggaaaa ttattgaagt tggagcggag gtcgaaggat
    16561 ttacggtcgg agatatcgtg ttttcccacg gcaggcatca aacgatcgtg tgcaagcatc
    16621 acaaaaagat ctttaaactt ccccaaaagc tggagcctga gctcggcatt ttctacacaa
    16681 acctgatgac ggcttataac gctgtattgg acacgagaat taaaatcggg gacgttgtcg
    16741 tcgtgtcggg actcggcgta gtcgggcagt taatcgctca attagccaaa ctgtccggcg
    16801 ccacagtcgt cggagtcgac ccgcttgaga acagactgaa catcgccagg gaagttgaaa
    16861 tcgactatgt gtttaatccg aatcggacgg atgtcgccaa ggaaatcaga gagattacga
    16921 ataaccgggg tgccgatgcc gtcttcgaag tatccggtca ttcaacggct ttgaatcaag
    16981 cgattcgcat agcggcgccg gacacggtaa ttacagccgt aggctggtat cagggcgggc
    17041 aatcggtgct gaatctttcc gaagaatttc accagaaccg gattacgatc cgcgcttcgc
    17101 aaactctcgg gatcgatcct tccatcagcc atatgtatga cgatgcgaga agaaggaata
    17161 tcgggaaaga tctgctgctt aagcttaaac tccgcaacct gatcagccac cggattccgt
    17221 tcggcgatgc gccgaaagcc tacgaaatga tcgataaaca cccgcatgaa gttctgcagg
    17281 tcgttctgac ctactcttaa gggggaagcc catgatcgtt ttcataaccg gcgcgaaccg
    17341 cgggctggga ctgagtcttt gcgaggtttt tctggaacgg ggacatcaag ttcttgccgg
    17401 agtgaggaac atgaacagag ccgggcagct gctcccgtta aaaaccaaat acaaggaaga
    17461 gctgagcatc attcccgtgg atgtaagcag ccaggattca atccggcagg cttattccct
    17521 gattaccgcc gtgacgccaa agcttgacgc gattatcaat aatgcggccc gtgaaggcat
    17581 ccgatgcaaa aagatccagg aagtggacat cgcggatttc gaagaaacgt ggaaaacgaa
    17641 cgttttgggt ccgatggcca ttatcaaatc tcttaagcct atgatggaaa gcagctcggt
    17701 caaaagcatt cttaatatca gctccgaggc cggaagtatt tccgagcttc ctttgaaaac
    17761 gtacggctac tgtatctcca aagcggctct gaatatgttt acccgtatgc tggagaggga
    17821 actgaaaccg gagaacttta aggtggttgc ggttcacccc ggctggatga aaaccgaaat
    17881 ggggggagag gacgcccctt acgaacccgg tgaaatcgcc cggcatttgt acgaacgcct
    17941 cacggaatcg cccgggaaaa aggctgattt caacatcgtc gatcatctag gaaaccgcat
    18001 gcccccgtaa gtgagtttaa ggcgatgatt agctggacga aagcttttac caagccgtta
    18061 aaggggagaa ttttcatgcc caacttattt gtctacggga ctctgcgtga gggagaaaac
    18121 aaccacaagt atatgaaaga agccaccctg ctttcgcgaa aggccagcat agccggttcc
    18181 ttggtagata ccggcaacgg ttaccccggc ttactgctgg aaaatcagct ggtggcgggc
    18241 gagtggtatg aagtctcgga agaaacgctg aaacggatag atgagctgga agagtatttc
    18301 ggtcccgggg atacaagaaa cttatttgac agaatcgaat gccaagtaaa cgagagcggc
    18361 ggaacacatc tgggttggac gtacgtgtac aaccgggacg attacctgga aacaaggttt
    18421 tccgactgga aacaataccg gctgcagcat gcttccggaa tagaggagaa gcaagatgtg
    18481 cctcactcgt tatgacgaga aatttttcga ttgcagaaaa tcgcagataa tcgcttattt
    18541 ggactcacaa caggtccccg tcattcctct attttataac agttatcaaa gcacagcgga
    18601 aatttaccgc cagatcttta tcgaaaataa gagcaaatgg aagtattcag agccttcttt
    18661 ttccgacgat gatttactaa gaaaaggaat taggcctgtt cgggcttctt ttcccgactt
    18721 cagccaggct tcggattgtt taaaggatct gctggcccgg cataaactcg ttttcgtatg
    18781 gggggacgag tattgtctgc cttaccggaa ggaagccttc caggccattc attccactca
    18841 ttcgcttgtt gtcacgggct atgacggtga gaacaaggca tactacgtgg aagattggga
    18901 cgggctctac ggatatttgc cggctgtaca tttggaagcg gctttcgaca gtctgtccag
    18961 gcagatgagg acattgttgg ttctggaact gaacgacgag gaaatgcggg aaaacaaaca
    19021 agaggataca gatttgttcc ggaagtggct gcaagccttc gaggacgact atatcttcta
    19081 cgaccgggtg ctgctggata tgcgcgatta tgaggaaaac cggctgatct cgatggatca
    19141 cggacttcgt ttaatcgccg catcccggca tgttttctcc aagtttcttc attatataga
    19201 tgacgccccg gaggaggtcg gcctccttat ccgcaaccat caactcgcta accatatcgc
    19261 ggccatcgtc aggcggtaca ttatcgccaa acagattgat tgggacgggg cagcttgcaa
    19321 gattcggcaa ttaagagagc aagaggatga ctttatgcgg aagctaaaga gccgttacgg
    19381 ctaattacgg cagcaaaagc ggataaaccg ctgtgcggaa accgccatcc catacagaag
    19441 gagttgaatg tgatgtcttt agaaactcag gaaaatgttc ggaccgctca aattgtatct
    19501 tgcgtgaatg aaattctgca gacgaaggaa ttcattaccc cggaaaccga tttgtttctg
    19561 cttggcttgg actcggtaac cgccgttaat ctgatcgtca gtttggaaag ccatttcggg
    19621 atcacgttta acgacgacga actgatcgtg gaaaattttt cgacggtgcg gaaaattacg
    19681 gaacatgtga cggtcaaact tgaggaagcc ctatgaagtt cactcatccg ctggattatt
    19741 atcgtctgaa cggcaaacaa atcctgtggt acatgaatat cggcgaagat caggacagcc
    19801 aggcgtcgaa ttactttccc tccgtcaagg accctcaatc cgagaagatt gtcgtgcagc
    19861 aggagcaaca gcttctcttt ctggcgaggc cgcaggacac ggtatttttt catacgatgc
    19921 cggagcaagc ctttctcgat tattggaaag agcgccgcct ctccttgccg tcgataattt
    19981 gttgcgacaa actttcgcaa gtgcctgatc ttgagcgtta tacgattatc ccgtttattg
    20041 tttcggatca actgcttgag ctgaagcggc ggtatccgca tatggatatc attgcgccgg
    20101 atttggcggt ctgccgggaa atcaatcata agttcaatac gagaagactt atggagagga
    20161 acgggtttaa cgtcacaacg ggttatttct gcagcgatat cgagagcttg gagcatgcct
    20221 acgaacagct catttccgcc ggcttttcca aatgcgtgtt gaaagttcca tacggctcgt
    20281 cgggtaaagg tctcaaagtc atcgacaacg aacggaattt ccgttttttg ctgaattata
    20341 ttcaaaaccg ccagacaaac gtcgacctgc tcctggaagg ctggcatccc caccgtctca
    20401 gcctgacgtc tcagcttttc attaccgaat atgaagtcca tcttctggcc gtcaccgagc
    20461 aaatcataga cccgaacggt gtctataaag gaacgaattt tacgcctgcc ttatcgcaat
    20521 cggaagcggc cgattatcgt gaagaaatct tacgggcagg agaactgata cggcagatgg
    20581 ggtatagggg cgtgcttggg atcgattcga tcctcgatac gaacggagaa ctgattccgg
    20641 ttatcgagat taacgccagg ctcactcagg tcacctatat tcttccgttg gttatcgaac
    20701 agaaaaagcg ttatgagttt gtcgaaagca gggtgctggt gtttaattcc agagcggatc
    20761 tggattttga agattacgaa aacgatctct cggaagtgac ccgcgattta ccggtccgaa
    20821 tcgatcttta caatttctgc aaggcttcgg gagcctttaa aaacacctat aagctctttg
    20881 tgttagtttc tgcccacaat tccgaacagc tgataaaagc ccgaagcctg cttgacgagc
    20941 tgaacacgaa aatgacaacg gctgttcatt aactcatgaa gaagggaggg gatttgcgga
    21001 tgaacctgga tcaagctgaa atcacggcct taacgaaaag gtttgagact cctttttatt
    21061 tgtatgacgg cgattttatc gaagcgcatt accggcaatt aagaagccgg acgaacccgg
    21121 cgatccagtt ttatttgtcc ctaaaagcaa ataacaatat tcatctggcc aaattgttcc
    21181 ggcagtgggg acttggcgtc gaggttgcat ccgccggaga actcgcttta gcccggcatg
    21241 ccggattttc agccgaaaac atcattttct cgggaccggg gaagaagcgg agcgagctcg
    21301 agattgccgt tcaaagcggg atttactgca tcatcgcgga atccgttgaa gaactctttt
    21361 acatagaaga actggctgaa aaagaaaaca aaacggcccg cgtcgcgatc cggatcaatc
    21421 ccgataaaag cttcggcagc acggccatca aaatgggcgg agtaccgaga cagttcggaa
    21481 tggacgaatc catgctggat gccgtaatgg atgccgtccg ctctcttcaa ttcaccaaat
    21541 ttatcgggat acacgtatat accggaaccc agaatctgaa taccgacagc attatcgagt
    21601 cgatgaaata caccgtagat ctaggcagaa acatttatga acgatacgga atcgtctgtg
    21661 aatgtattaa tcttggcgga gggttcggag tcccgtattt ttcacacgaa aaggcactgg
    21721 atatcggcaa aatcacgcgt acggtcagcg attacgttca agaggcgagg gatacacggt
    21781 tcccgcaaac gacctttatt atcgaaagcg gaagatactt attggctcaa gcggccgtgt
    21841 atgtaaccga ggtgctttac cggaaagcgt ccaaaggaga agtttttgtg atcgtagacg
    21901 ggggaatgca ccaccatgcg gcctccactt tccggggaag aagtatgcgg agcaactacc
    21961 ccatggaata tattcccgtt agggaagact ccggccgtcg cgaactcgaa aaagtcacca
    22021 ttgccggtcc tctctgtacg cccgaagatt gtctagggaa ggatgtccat gtgccggcct
    22081 tgtatccggg cgacctcgta tgtgtgttga actcgggagc ttacggactg agctttagcc
    22141 cggttcattt tctggggcac ccgacgccca ttgaaattct caaacggaac ggttcatacg
    22201 agctgatcag acggaaagga acggcggatg atatagtcgc cacccagctg cagacagaat
    22261 caaatctcct ctttgtggac aaatagaaag cccaatatac gaaaagcagg gggatgattg
    22321 cattggatac gattcagtat tatggccaaa ttccaggcgt caaccattat aacggcaaga
    22381 aagaatttat ggaaaacgcc gttaaaatcg cgcagctgtc cgatgcttac ggcatagtcg
    22441 ggtccctgtc gttttttaac cattccgtat tggacccttg ggccgtaagc tcggttatta
    22501 tgcggcatac ggaacgtcat gttcctctaa ttgcgcttca gccctacatg tatcctccct
    22561 atacggctgc caagttaatt cagagcttca cttatttgta cgaccggcgg atcgacttga
    22621 atatgatcac cggggccgtt accggcgagc tccagcaaac cgggggctat atcgatcatt
    22681 cgtcccgcta caagaaactt cacgaatatg ttcaggtgct ccgtctgctg cttgaatccg
    22741 attctgccgt ttcgttcaaa ggggactatt atgagctgaa taatttggag ttcaagcctt
    22801 tgctgccgga caaacggctt ttcccgcgca tatttatgtc cggttcttcc gaggaagggc
    22861 ttgaaacggg tttgaaagcg gcggatttcg tcgttactca tccgggaccg ctggaacatt
    22921 ttaaacgcca ttttagcgaa aaggtgcagg gatcggctgt tcaaagtgcc ataagaatcg
    22981 agattatcgc cagagaatcg gcggagcaag catggaagat cgctcatgcc cgttatcccg
    23041 gcaaccggca ggggaagatc cagctccgga tgaaaacaaa ttcggaatca agctggcagc
    23101 ggatgctggc cgaactggct ttagcgagtg aaacgtacga tgaagtcttc tggatgggcg
    23161 ggtacatgaa cggcggaatt tacagtcccg tgttagtcgg ggattacgag caagtggccg
    23221 cttatttgaa tgagtattac aaactcggcg tgaaagccgt gttgctgggt tccatgtaca
    23281 gcgaggagga ttttattcat ttcagccggg tgaaggaagg aatttcgaat ccggtctagg
    23341 gaccggaccg cgagcaaaca atccgacgga aggagagata gcatgcgatt gattttaatc
    23401 cgtcatgcgc aggcccgctg caatattctg gaggacgacg ctcttatgga cgcttacgat
    23461 cctcattgcg agctgacgga agcggggatc ggccaggccg tgaagctccg ggacgaatac
    23521 ccggtttctc tgacgccgtc ggtcatttat tccagccctt tgaaaagggc gagggaaacg
    23581 gcgggcattt tccgcgggcg ctatccgtcc gtgcctttcg tcgaagacga acgtttgagc
    23641 gagctgaaag ctccggagtc ttttatcccg ccgattacgc aggggcagtg ggatctgtat
    23701 ctggagcagc gtatccggtc gcctcatttg gaaatcgtca aggggctaga gtcgcttgac
    23761 gtacagcggg aacggatcga gcggttctat aaggatctgt tccggaagta tgccgaagaa
    23821 gcgtgcaaca tcgtcatttt tacccacgct ttcagcatcc agctgtccat cctgtttttt
    23881 ctcggattag gaaacgagca gctcctgcaa tggcaaatca aagcaagcaa tacggcgatg
    23941 cacatcattc attacgaccc gaccagcggc agttttctac tggagagtct gaacaaccgt
    24001 tcacatctcc aaacaaccgg ataagaaagg atgaaatgag atggataaca aagagcggaa
    24061 cacgctgtat caagtggtct acgcgatttc cggcgtcacc ggcgaggacg gggacgagct
    24121 cgtggaaacg tttaaacagg gaccgatgga agtggataag cgggatttct ttgaaatcgt
    24181 tcaacgggtc gaatcactgt tcgactgcac gctcgatatg aatttggaag gtccttatct
    24241 gatacatgcc gatgaaatcg tgactaagat tactaagcta aacgtttagt cctggccgtt
    24301 gaaataagca agtaggagga atcaccttga ccaagcaatt cgatgtatgc gtgatcggca
    24361 gcggagcaag cggttccgtt gtcagcaaat atttggccga gaaagggttg gaagtcgcca
    24421 ttgtggagca aggcggtctc attgaaccgg gaacgaatat agattcggtc atgagccatg
    24481 cggagaaagc ctacgcgaga gagtcggacg gaacctgggg gaataacggc tatccgtggt
    24541 ctgccagcgc ggttggcggc ggaacgatat tttatgccgg tatctcattc cgatacaaag
    24601 acgtcgactt cgatgcgagg gaatatgtgg cgtccgatgc tctggacccc cagtggccca
    24661 tcgggtattc ggatttggac ccctactaca acaagatcga acagtggctt ggggtgtccg
    24721 gggatttgaa tgccgatccc ttcaaaaaca gtgccggtac cggttcgctc cggacggccc
    24781 attcctacag ccagcaggga gccctgatcg accaggcggg caaagagctc ggcctgcatc
    24841 cgtttccgac tccgctggcg gttaacacaa cggatcataa cggctatccg ggctgcgaca
    24901 acttaaccag ctgcacggat tacgcgtgcc cgatcggcgc caaagcggac agttttacgc
    24961 gtattttgaa gcccgtgttg aagctggagc atgtgacgct gatgaaaaac acgaaggctc
    25021 tggcgttcca tcagacggac ccgtccgtaa ttgactcttt ggaatgcctg gatttggaca
    25081 cgaaagaacg gttccacatc agagcgaaac aattcgttct ggccggtaac gccatccaaa
    25141 gcgccgcgct cgtgctgcgt tcgacgaaca aatggtggcc ggacggagta gggaacaaat
    25201 ccggtctggt cggctcggga ttaagtttca agaacagcga atacgtatcc ggctgggtgg
    25261 acaatcatcc ttacgagcag ttgaacgaac cgctgaaagg attgtactcc accgtttcga
    25321 ttacggatta ttaccaggac ccgcattgcc attccggcct gggcgggctg atctatgaag
    25381 ccaatccgtg gacctattac ggccatgaag gcagcggcat gtatgttcaa ttggaatgta
    25441 tcatagcgga ccagcctatg gtgaccaata aagtccggct gtcgaacgat aaagaagcca
    25501 cgggggtacc caaaattgtc cttgattatc agacacatga atgggacaag cgccggctga
    25561 gctttatgat cgagagggcc ggagaaattt tgacggccat gggagccaag cgggtacaat
    25621 gcaacccttc ctactactat ttaggaagcg ctcatttgca tggaacttgc cgagcaggca
    25681 cggatgagcg gacttccgtt gtcgataagt tcggccgatt ccacacgatc gacaatttgt
    25741 ttgcggccga cgggtccttc atgccttttg cgggcggtgt taatccaacc ttgacgatcc
    25801 aggcgaacgc tctaagaata gccgaaaata tgagcgtctg attacgggcg acaaccgggc
    25861 ggcacgggaa atccggaagc ggggaagtga tcgttatcgt ttcagttttg gtgaggctcg
    25921 gctggttttt tagagagcat aaaatccggt atacgctggc ggtactcatc ctggtcctcg
    25981 taaacggggt cgaaatgctg ccgcccctgc tgctgggctc cgctcttgac catatccagc
    26041 aaaacacgct gaccccggcg agtctggcgc agtatatcgg atttattctc ggactctccg
    26101 tcgtcatcta tactctgaat tacgcttgga tgtttcaatt gcacggcgga gcgaaccgtt
    26161 tgcagctcaa aatgcgttcg cgtttgatgg cccatctgtt aaagatgaca ccgccctttt
    26221 tcgaaaagta ccggaccggc gatctcatgg cccgggcgac caacgatatc aaatccatcg
    26281 gagaaacgtc gggctccggc attttgacct tagtcgactc ctgtttgttt tcggtgacga
    26341 tcctgattac gatgggcttt tttatcgact ggaatttaat gctggtcacc atcttgccgc
    26401 ttccgcttat cgcggcggtc attacctttt tcggaaaata ccttcaccgc cgttttacga
    26461 tcgctcagga tgctttcggg gcgcttaacg atcaagtgct ggagaccgtg accggtattc
    26521 gcgtggttag agcttatgtg cgggaagagg cgaatcagaa aaatttcgaa accatggcgg
    26581 aagatctgtt ccggaaaaat gtcgccatcg cgcgggtcga agctttattc gagcccacca
    26641 tccgcatgtt aatcgggatc agttatttga tcggtctcgg atacgggacc tatcttgttt
    26701 ttcagcagcg catttcactg ggagaactga ttacgttcaa cgtctatttg ggcatgctga
    26761 tctggcccat gtacgcgatc ggggaactga ttaatattat gcagcggggg agcgcttcct
    26821 tggaccgttt ggatgaaatc atgacctatc cgcccgacgt acgggatggg gaagatccgg
    26881 aagaggacat cgcgctgccg cataccctgc aatttcaccg cgtaagcttc cgctatccgt
    26941 cttcccaaac cgataacctg aaagacatca cgctgcatat taaccgcgga cagaccatcg
    27001 gtatcgtcgg acggacagga agcggcaaga cgaccctgtt aaggcagctg ctcagggagt
    27061 atccgacaga caggggcgaa atcaccgttt cgggcatccc gctggaacgc attcggcttg
    27121 aagcctggaa tcgatggatc ggttatgtcc cgcaggagca gctgctgttt tcgaaaaccg
    27181 tcaaagaaaa tgttcgattc ggcaaggaga actcgaccga cggggaagtg ttccgggctc
    27241 tggagctggc ttcctttatg gaggatattc atacgcttcc ggacgggatc gatacattag
    27301 taggggaaag aggggtgtcc ttgtcgggcg gacagaagca aagaatcgct tttgcgcggg
    27361 cgatcatttc cgaacccgaa attatgctgc tggatgacgc tttatccgct gtcgacgcta
    27421 aaacggaggc gggcataatc gccaacctta ttgcggagcg aaaggggcgt acgacgctca
    27481 tttcgacgca ccggttatcc gccgtggaac attccgattt catcgtcgta atggatcagg
    27541 gccggatcgt ccaatgcggc gcgcatgttc aattagttgc ctgcgaaggt tggtataaag
    27601 aacaatacga gtcgcaaaaa ctgcaaatga acttgctaaa gtgagcgtgg aaaatcctat
    27661 gaatgtagga acgaagctgt tcaagtatgt tctttattac aagaaatcgt ttatcggggc
    27721 gctttgtctt ctcttgctga tggtagcggc ggaattgtcc ggaccaatta tcgccaaacg
    27781 gttaatcgac caacatattc tggggataga aaaaccttgg tacgaaacac gggaacccgg
    27841 ggaaaatgcc gtgccttata aagggaagca ttacaaaaga ggcgattatt ttcacgagca
    27901 ggaagagaag ggacaagaag tccgcattct ccaggtgcag cggcagttcg tatttgttcc
    27961 ggaagctgtg aaggcggacg gtgaacggat tgtacaaggc gaccgactca ttatcaagcg
    28021 aaatgcggaa accgggacgt acccggccga tattctctcc acgcgcgact tgtttctctt
    28081 ttatgaaccg gaattggaga aaatcgtcaa cttgatcctt ctttatttcg ggttcgtagg
    28141 ggcagccagc ttgctcgaat acggccaaac gtatgccttg caagtctctt ccaaccgtgt
    28201 cattcaaaaa atgcggatag atgttctaaa gcaaatccac cggattccca tttcgtactt
    28261 cgaccgaata ccggccggcc ggatcgtatc ttgcgtaacc aacgataccg aagccgtgaa
    28321 agaattgttt gttaccgtat tgagaaattt tttcacggga accgtctaca tcaccggaat
    28381 cttgacggct ctctttatat tggacgtcag gctcgcgctt atttgcacgc ttatcattcc
    28441 tgcccttgcg ggctggatct gcctgtatca gaaatttgca tccaaataca gccaaaccat
    28501 ccgatccacc ttgagcgata tcaacggctt gatcaacgaa accattcaat gtatgcctgt
    28561 cattcgcgcg ttctcccggc agcgcaccgt tcggcaggaa tttgaggata aaaatgtacg
    28621 ttactttacg tattccaata aactgcttac tttaaaagcg ctcacttcca ataatctggt
    28681 tacggttctc agaagtctcg cgctcgccgc catcatctgg tatttcggca gcgccggttt
    28741 gagccatcag tcggtcttca cctttggcat gatttatgcc tttgtggaat atgtggagcg
    28801 cttattccag cctctggtgg gaatggtgaa tcaactggct cagctgaatc attccctcgt
    28861 ttcggccgaa cgggttttca ggctgatgga agaggaaggc tcggatgtcg cagacgagga
    28921 aacgccgaga atgaaaggga acgtaaaatt cgagaacgtt tcctttgcct attcggacga
    28981 tcattacgtt ctccgagata tttctttcga ggcgaagcag ggacagacga tcg
//
