LOCUS       BGC0000706             15046 bp    DNA     linear   BCT 15-JUN-2006
DEFINITION  Streptomyces kanamyceticus kanamycin biosynthetic gene cluster,
            clone: pKM7.
ACCESSION   BGC0000706
VERSION     BGC0000706
KEYWORDS    .
SOURCE      Streptomyces kanamyceticus
  ORGANISM  Streptomyces kanamyceticus
            Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
            Streptomyces.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-07 20:21:07
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..15046
                     /clone="pKM7"
                     /db_xref="taxon:1967"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces kanamyceticus"
                     /strain="21-18"
     subregion       1..15046
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000706"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..15046
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     CDS             351..1595
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045:glycosyl transferase group 1 (Score: 271.3;
                     E-value: 2.6e-82)"
                     /gene_kind="biosynthetic-additional"
                     /note="orf20"
                     /product="glycosyltransferase"
                     /protein_id="BAE95600.1"
                     /transl_table=11
                     /translation="MRVLRLTPFYHHDCVDSWPAEFDAVGGMQVQILRMSRALAELGVR
                     QQVLTVGFPGLPRVRRDSENLVVRITRAPLPRLRSRITGLVGLNQAWLAAALTECVKLR
                     RRWPADLIQVHLDGQLWALLAGPVAARLVGVPYTVTVHCSRLAVYQPMSTVDRIQHPLV
                     TAVERWALRRAAGITTLTERTATVLAAELGAAQRVIDVVPDAVDPDRAEAAPAEVERLK
                     KRFGLPQEGGPVIGFVGRIAHEKGWRHAVQAVAELADAGRDFTFLVVGDGPQRADMEAA
                     VAEAGLTDRFVFTGFLPNDEIPAVMTALDVLLMPSVHEELGGSAVEAMLAGTPVAAYGV
                     GGLCDTVGKVTPSLLAAPGQVAELARTVKRVLDDPAPVLAELRAGREWLADEFGVHHAA
                     GLALAHYERVLGKER"
     CDS             1654..2661
                     /codon_start=1
                     /note="orf21"
                     /product="conserved hypothetical protein"
                     /protein_id="BAE95601.1"
                     /transl_table=11
                     /translation="MRQDWQHLDESDVRVRPGRRSSRPRSKNRPEHADARSAMVVGVDR
                     GRWTCVLDSDPESRITAMRARELGRKSAVIGDRVDVIGDLSGKPGTLARMVRVARRDSV
                     LRRTADDTDPYERVVVANAEQLVIVCALAAPQPRAGFIDRALVAAYAGGLRPVLCLTKA
                     DLKGPEDVVSTYAHLDLSIVVTRSDRAPDALREQLAGRVSALVGHSGVGKSTLINILIP
                     QARRVTGEVSAIGKGRHTSTSAVALPLPDGGWAVDTPGIRSFGLAHVTSDDVLDAFPDL
                     SDGAEDCPSGCRHLGAPHDSGCGLERLSEADPARARRLESLRRLLRSHAGIEEE"
     CDS             2696..3094
                     /codon_start=1
                     /note="orf22"
                     /product="conserved hypothetical protein"
                     /protein_id="BAE95602.1"
                     /transl_table=11
                     /translation="MSRLTHISAPEGVAPATAYTHVVMGTGRFVAISGQLALDEDGKLV
                     GEGDAAAQAHQVFENLRRCLTAAGATFDDVVKLTYFVTDMAHMRAIRGARDAFLDPERL
                     PASSAVQVGALVAPEYLMEIEAFAVVAE"
     CDS             complement(3118..3687)
                     /codon_start=1
                     /note="a homologue of SCO6476 and SAV1908; orf23"
                     /product="conserved hypothetical protein"
                     /protein_id="BAE95603.1"
                     /transl_table=11
                     /translation="MDEELRTVTERLRAEAGGSKEYERLVATGDLEELAEVLVSPGQPL
                     WTRELAAVLLGVAGDRRAFESLVLLLNHRDAPRCAAAAYALARLGDPRTARAAAALATN
                     ELRVAYALHPVRLLTELRAPESAPALITTLRRRLAPHDPFGKIALACVEGLGALGDPRA
                     RPVLTAARAHPRLAAAASAALARLPE"
     misc_feature    complement(3652..3654)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     CDS             4042..5823
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1095:3-hydroxybutyryl-CoA dehydrogenase (Score: 304.4;
                     E-value: 1.1e-92)"
                     /gene_kind="biosynthetic-additional"
                     /note="a homologue of SCO6475 and SAV1909; orf24"
                     /product="putative dehydrogenase"
                     /protein_id="BAE95604.1"
                     /transl_table=11
                     /translation="MDAPLNTVAVIGLGTMGTGIAEVLARAGREVIGIDISQAAATRAV
                     TALETSTARAVRRERLTDEERRGILARFRTFTDLQAAADVDLVIEVVPESYEIKQRIVR
                     ELDAVVRPETIIATGTNALSVTRLAAESARPERVLGLHFFNPAPAMKLVEIVSSVLTAP
                     AAVAAVTELAYALGKEPVAVGDRPGFVADGLLFGYLNQAAAMYEAKYASREDIDAAMKL
                     GCGLPMGPLALLDLIGVDTARTVLDAMYAESHDRLHAPAPVLKQLSEAGLTGRKSGRGF
                     YTYAEPGSTEVVRDALTPPEGERSVPSRAVRAVGVAGSGTMASGIAEVFAKAGYDVVLA
                     ARSEEKAQLARARIGKSLARSVEKGRITAESAAETLERITPAGSYEAFADVDLALEAIA
                     EDLDVKQQLFATLDKVCKPGAILATTTSSLPVVACARATSRPRDVIGMHFFNPAPAMKL
                     VEVVRTVLTSDEVHATVREVCAAIRKHPVDCGDRAGFIVNALLFPYLNNAIKMVQEHYA
                     TLDDIDAAMKLGGGYPMGPFELLDVVGLDVSLAIEKVLHREFRDPGLAPAPLLEHLVAA
                     GCLGRKTGRGFREYARR"
     CDS             5936..6745
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1215:TetR family
                     transcriptional regulator (Score: 199; E-value: 1.4e-60)"
                     /gene_kind="regulatory"
                     /note="a homologue of SCO6474 and SAV1910; orf25"
                     /product="putative transcriptional regulator"
                     /protein_id="BAE95605.1"
                     /transl_table=11
                     /translation="MSKAAKSSRNASPDAPESAAGSRAAAQRLKMRRELAAAAMELFAT
                     KGYEATTVDEIAAAAGVARRTFFRHFRSKEEAIFPDHDDTLVRAEEVLNAAPAHEHPLD
                     TVCRGIKEVMKMYAASPAVSVERYRLTREVPTLREREIASVARYERLFTRYLLGHFDEQ
                     AHHDGNDDPLLAEVAASAVVTAHNHVLRRWLRAGGQGDLEAHLNHAFGIVRLTFGTGIG
                     AGRSSAPATAPATVSAQGEVLVTVARTDAPLDEVMRTIEKALRERAS"
     misc_feature    6192..6197
                     /note="conserved hexanucleotides; recombination site RsB"
     CDS             7143..8480
                     /NRPS_PKS="Domain: PKS_ER (52-413). E-value: 1.3e-25.
                     Score: 82.0. Matches aSDomain:
                     nrpspksdomains_BAE95606.1_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1028:crotonyl-CoA reductase / alcohol dehydrogenase
                     (Score: 334.2; E-value: 1.3e-101)"
                     /gene_kind="biosynthetic-additional"
                     /note="a homologue of SCO6473 and SAV1911; orf26"
                     /product="putative crotonyl CoA reductase"
                     /protein_id="BAE95606.1"
                     /transl_table=11
                     /translation="MKEILDAIQSQDAQSADFAALPLPDSYRAITVHKDETEMFAGLDT
                     RDKDPRKSLHLDDVPVPELGPGEALVAVMASSVNYNSVWTSIFEPLSTFGFLERYGKVS
                     ELTKRHDLPYHVIGSDLAGVVLRTGPGVNSWKPGDEVVAHCLSVELESSDGHNDTMLDP
                     EQRIWGFETNFGGLAEIALVKSNQLMPKPGHLSWEEAAAPGLVNSTAYRQLVSRNGADM
                     KQGDNVLIWGASGGLGSYATQFALAGGANPICVVSSEQKADICRSMGAEAIIDRSAEGY
                     RFWKDERTQDPKEWKRFGKRIREFTGGEDIDIVFEHPGRETFGASVYVTRKGGTITTCA
                     STSGYMHEYDNRYLWMSLKRIIGSHFANYREAWEANRLIAKGKIHPTLSKVYSLEETGQ
                     AAHDVHRNAHQGKVGVLALAPKEGLGVRDPELRAQHIDAINRFRNV"
     aSDomain        7299..8381
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BAE95606.1_PKS_ER.1"
                     /evalue="1.30E-25"
                     /label="BAE95606.1_PKS_ER.1"
                     /locus_tag="BAE95606.1"
                     /protein_end="413"
                     /protein_start="52"
                     /score="82.0"
                     /tool="antismash"
                     /translation="SLHLDDVPVPELGPGEALVAVMASSVNYNSVWTSIFEPLSTFGFL
                     ERYGKVSELTKRHDLPYHVIGSDLAGVVLRTGPGVNSWKPGDEVVAHCLSVELESSDGH
                     NDTMLDPEQRIWGFETNFGGLAEIALVKSNQLMPKPGHLSWEEAAAPGLVNSTAYRQLV
                     SRNGADMKQGDNVLIWGASGGLGSYATQFALAGGANPICVVSSEQKADICRSMGAEAII
                     DRSAEGYRFWKDERTQDPKEWKRFGKRIREFTGGEDIDIVFEHPGRETFGASVYVTRKG
                     GTITTCASTSGYMHEYDNRYLWMSLKRIIGSHFANYREAWEANRLIAKGKIHPTLSKVY
                     SLEETGQAAHDVHRNAHQGKV"
     CDS_motif       7815..7877
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BAE95606.1_0001"
                     /evalue="1.80E-03"
                     /label="PKSI-ER_m5"
                     /locus_tag="BAE95606.1"
                     /protein_end="245"
                     /protein_start="224"
                     /score="11.1"
                     /tool="antismash"
                     /translation="GDNVLIWGASGGLGSYATQFA"
     CDS             8503..10551
                     /codon_start=1
                     /note="a homologue of SCO6472 and SAV1912; orf27"
                     /product="putative coenzyme B12-dependent mutase"
                     /protein_id="BAE95607.1"
                     /transl_table=11
                     /translation="MTERQKDRPWLMRTYAGHSTAEASNELYRRNLAKGQTGLSVAFDL
                     PTQTGYDPDHILARGEVGRVGVPVSHLGDMRRLFQDIPLDRMNTSMTINATAMWLLALY
                     QVVAEEQGADITQLQGTTQNDIVKEYLSRGTHVFPPGPSLRLTTDMITYTVAHIPKWNP
                     INICSYHLQEAGATPVQEIAYAMSTAIAVLDAVRDSGQVPEEKFGDVVARISFFVNAGV
                     RFIEEMCKMRAFGRIWDQITRERYGIENPKQRRFRYGVQVNSLGLTEAQPENNVQRIVL
                     EMLAVTLSKDARARAVQLPAWNEALGLPRPWDQQWSLRIQQVLAHESDLLEYEDIFAGS
                     HVIEKKVDELVTDSLTEMDRIQEMGGAMAAVESGYLKSQLVSSHAERRARIEAGHEKIV
                     GVNVYESTETNPLTADLDAAIMTVDHAVEAKVVRAIGDWRTTRQESSDRQGMGDPFHYP
                     TTGQALERLKEAAAGTENLMEATLECARAGVTTGEWSGALREVFGEFRAPTGVSSAPVA
                     VAAEEGSPMAEVRRKVALTGEELGGKLRLLVGKPGLDGHSNGAEQIAVRARDAGFEVVY
                     QGIRLTPEQIVSAALAEDVHCVGLSILSGSHAELVPDVLVRMREAGATDVPVIVGGIIP
                     NADAEDLKRAGVAAVFTPKDFGITEIIGRIVDEIRKANKLDPLEVPA"
     CDS             10548..11513
                     /codon_start=1
                     /note="a homologue of SCO6471 and SAV1913; orf28"
                     /product="putative citratelyase"
                     /protein_id="BAE95608.1"
                     /transl_table=11
                     /translation="MTAPINRLRPRRSCLAVPGSNPRFLEKAQGLPADQVFLDLEDACA
                     PLAKPEARHTIVKFLNEGDWTGKTRVVRVNDWTTEWTYRDVVTVVEGAGQNLDCIMLPK
                     VQTADQVVALDLLLTQIEKTMGFEVGKIGIEAQIENAQGLNNVNAIAEASQRVETIIFG
                     PADFMASINMKSLVVGEQPPGYPADAYHFILMKILMAARANNLQAIDGPYLQIKNVDGY
                     REVANRAAALGFDGKWVLHPGQVEAANEVFSPSQEDFDHAELILDAYEYYTSEAGGKKG
                     SAMLGDEMIDEASRKMALVISGKGRAAGMQRTSTFEAPEA"
     CDS             11518..12030
                     /codon_start=1
                     /note="a homologue of SCO6470 and SAV1914; orf29"
                     /product="conserved hypothetical protein"
                     /protein_id="BAE95609.1"
                     /transl_table=11
                     /translation="MQFGRTYEEFEVGAVYKHWPGKTVTEYDDHLFCLLTMNHHPLHMD
                     NNYAEKTTDFGKNVVVGNYIYSLLLGMSVPDVSGKAIANLEVESLKHVAPTFHGDTIYG
                     ETTVLDKTPSKSKSDRGIVYVETKGYKQDGTLVCVFRRKVMVPTETYIKERGGEQPGRP
                     ELKSQEK"
     CDS             12033..13238
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1006:acyl-CoA dehydrogenase (Score: 371.9; E-value:
                     6.2e-113)"
                     /gene_kind="biosynthetic-additional"
                     /note="a homologue of SCO6469 and SAV1915; orf30"
                     /product="putative acyl-CoA dehydrogenase"
                     /protein_id="BAE95610.1"
                     /transl_table=11
                     /translation="MSRLAQTAGLTDIQQEILSTVRDFVDKEIIPVATELEHRDEYPQQ
                     IVDGLKELGLFGLMIPEEYGGLGESLLTYALCVEEIARGWMSVSGIINTHFIVAYMLKQ
                     HGTQEQKETFLPRMAAGEVRGAFSMSEPGLGSDVSAITSKGVKDGDDYVLNGQKMWLTN
                     GGTSTLVAVLCRSDEGHPEGTAPHKSMTTFLVEKEPGFGEVRPGLTIPGKIDKMGYKGV
                     DTTELIMDGLRIPANRVLGGATGRGFYQMMDGVEVGRVNVAARGCGVAQRAFELGVSYA
                     QQRHTFGKPIAQHQAIQFKLAEMATKVEAAHAMMVNAARKKDSGERNDLEAGMAKYLAS
                     EYCKEVVEDAFRIHGGYGFSKEYEIERLYREAPMLLIGEGTAEIQKMIIGRRLLEEYRF
                     QG"
     CDS             13409..14065
                     /codon_start=1
                     /note="a homologue of SCO6468 and SAV1916; orf31"
                     /product="putative phosphatidylserine decarboxylase"
                     /protein_id="BAE95611.1"
                     /transl_table=11
                     /translation="MPHSQTSAPRDGILQGRLARGASPWLLPTVATAALSLARARRSKG
                     AAAVAVPATALAAGMLWFFRDPEREIAQGRVISPADGVVQSIMPWKDGRTRVAIFMSPL
                     NVHVNRAPLAGTVTSVEHIPGGFVPAFNKESENNERVVWHFDTELGDIEMIQIAGAVAR
                     RIVPYVPQGSKVEQGERIGLIRFGSRVDIYLPEGVEVDVEVGQKTVAGVTRIDRG"
     CDS             14124..14906
                     /codon_start=1
                     /note="a homologue of SCO6467 and SAV1917; orf32"
                     /product="putative phosphatidylserine synthase"
                     /protein_id="BAE95612.1"
                     /transl_table=11
                     /translation="MPLSLRLSIADTLTLGNATCGFMAVYFTTTGILIPHLQGSNESGM
                     ARHSAATAVILMLCAAVFDLFDGLVARKLRSSPMGAELDNLSDLISFGLAPAYFVLVYG
                     MVADDAHQRVAAVGAIVVLLAVVLRLARFSCVTVKDGTFQGMPSPFGALTVVSIVLLEL
                     PFVATLLAIIGTAWLMVSRVEYPKPRGPLAVAMLSWIVAAMGLLAAWAFDAPGGQLLLQ
                     TGCALQVVLGAVIPLFATARRLNNFRDNRREARAAQLP"
ORIGIN
        1 ggatcccgaa ccggccgacg tcaccttcct gttcaaggcc gtcccctgcc ttgaggcaca
       61 gggcaagggc ctcggctgga agctgctcga ccagatcaat tcccccgtcc tggtggtgag
      121 tttccccacc aagaccctgg gccgccgctc caggggcatg taccagaccc attccgcgga
      181 attcgcccgg cgcgtcgccg aacggccctg gaagtgcgac gagatcgagt tcggcgacga
      241 actcgtctac gtggtcacga agggctgacg acgggcagga gccgttccca cgaggcgagg
      301 gcgccggacc agaacccatt gggctgaact aggcaagatg gaggtacagc gtgcgcgtac
      361 tccggctcac cccgttctac caccacgact gtgtggacag ctggcccgcc gagttcgacg
      421 cggtgggcgg catgcaggta cagatcctgc ggatgtcccg cgccctggcc gagttggggg
      481 tgcgccagca ggtgctcacc gtcggcttcc ccggtctgcc cagagtgcgg cgcgactccg
      541 agaacctggt ggtgcggatc accagggccc cgctgcccag gctgcgctcc aggatcaccg
      601 gcctggtcgg cctcaaccag gcgtggctgg ccgccgcgct caccgaatgc gtgaaactgc
      661 ggcgccgctg gcccgccgac ctgatccagg tccatctgga cggccagctc tgggcgttgc
      721 tcgcgggacc ggtcgccgcc cgcctggtgg gcgtgccgta cacggtcacc gtgcactgct
      781 cgcggctggc ggtgtatcag cccatgtcca ccgtcgaccg catccagcac cccttggtga
      841 ccgccgtcga gcgctgggcg ctgcgccgtg cggccgggat caccacgctg accgagcgga
      901 ccgccacggt gctcgccgcc gaactgggcg cggcccagag ggtgatcgac gtggtgcccg
      961 acgcggtcga ccccgatcgg gccgaggccg cgcccgccga ggtcgagcgg ctgaagaagc
     1021 gtttcggcct gccgcaggag ggcggtcccg tcatcggatt cgtcggccgg atcgcccacg
     1081 agaagggctg gcgccacgcc gtccaggcgg tggccgaact cgcggacgcg ggccgggact
     1141 tcaccttcct cgtcgtcgga gacggaccgc agcgggccga catggaggcg gcggtggccg
     1201 aggccggtct caccgaccgg ttcgtcttca ccgggttcct gcccaacgac gagatccccg
     1261 ccgtgatgac cgccctggac gtcctcctga tgccgtccgt gcacgaggag ttgggcggca
     1321 gcgccgtcga ggccatgctc gcgggaacgc cggtggccgc ctacggggtc ggcggcctgt
     1381 gcgacaccgt gggcaaggtg accccgtcct tgctggccgc gcccggccag gtggccgaac
     1441 tcgcgcggac ggtcaagcgg gtactcgacg atcccgcgcc ggtcctggcc gaattgcgtg
     1501 ccggacggga gtggctcgcg gacgagttcg gtgtccacca cgcggccggt ctcgcgttgg
     1561 cccactatga gcgggtactc ggcaaagaac ggtagccgta cgacacccga cgtcctgttc
     1621 gacttgttct acgacagctg atctggagta tgcgtgaggc aggactggca gcacttggac
     1681 gagtccgacg tacgggtccg accgggacgg cgtagttccc gcccgcgcag caagaaccgc
     1741 cccgagcacg ccgacgcccg gtccgccatg gtggtcgggg tcgatcgcgg ccggtggacc
     1801 tgcgtcctgg actccgaccc cgagagccgg atcaccgcga tgcgcgcccg cgaactgggc
     1861 cgcaagtcgg cggtgatagg cgaccgcgtc gacgtgatcg gcgacctgtc cggcaagccg
     1921 ggcaccctgg cccgcatggt ccgggtcgcc cggcgcgaca gcgtcctgcg gcgcaccgcc
     1981 gacgacacgg acccctacga gcgggtggtg gtcgccaacg cggaacagct ggtgatcgtc
     2041 tgcgcgttgg cggcgccgca gccgcgcgcc ggtttcatcg accgtgccct ggtggcggcc
     2101 tacgcgggcg gtctgcggcc ggtgctctgc ctgaccaagg cggacctgaa gggccccgag
     2161 gacgtggtga gcacctacgc ccatctggat ctgtcgatcg tggtcacgcg ctccgaccgc
     2221 gccccggacg ccctgcgcga gcagctggcc gggcgcgtct cggccctggt cggacactcc
     2281 ggagtcggca agtccacgct gatcaacatc ctgatcccgc aggcccggcg ggtcaccggt
     2341 gaagtgagcg cgatcgggaa gggccgccac acgtccacct cggccgtcgc cctgccgctg
     2401 ccggacggcg gctgggccgt ggacaccccc ggcatccgct ccttcgggct tgcccacgtc
     2461 acgtccgacg acgtcctcga cgccttcccc gacctgagcg acggcgcgga ggactgcccg
     2521 tccggctgcc gtcatctggg cgccccgcac gactcgggat gcggcctgga gcgcctgagc
     2581 gaagccgatc cggccagggc gcggcggctg gagtcgctgc gccgcctgct gcgctcgcac
     2641 gcgggcatcg aagaggagtg accaccgtga ccacctcgtg gtccaccatg gggacatgag
     2701 ccgactgacc cacatcagcg cccccgaggg cgtcgccccc gccaccgcct acacccacgt
     2761 cgtgatgggc acgggccgat tcgtcgcgat atccggccag ttggccctgg acgaggacgg
     2821 caagctggtc ggcgagggcg acgcggcggc gcaggcccac caggtcttcg agaacctgcg
     2881 gcgctgcctg accgcggcgg gcgcgacgtt cgacgacgtc gtgaagctga cgtacttcgt
     2941 gacggacatg gcgcacatgc gggcgatccg cggcgcgcgg gacgccttcc ttgaccccga
     3001 gcggctgccc gcttcctcgg cggtgcaggt gggcgcgctg gtcgcgcccg agtatctgat
     3061 ggagatcgag gcgttcgcgg tggtggccga gtaggcgcgc gccgaccgcc cccgacctca
     3121 ctccggcagc cgcgccaggg ccgccgaggc cgccgccgcg aggcggggat gggcccgcgc
     3181 cgcggtcagc accggtctcg cccgggggtc cccgagcgcg cccaggccct ccacacaggc
     3241 gagcgcgatc ttgccgaacg ggtcgtgcgg ggcgaggcgc cgccgcagcg tcgtgatcag
     3301 cgcgggcgcc gactccgggg cgcgcagctc cgtcaggagg cgcaccgggt gcagggcgta
     3361 ggcgacgcgg agttcgttcg tcgccagggc ggccgccgcg cgcgcggtgc gggggtcgcc
     3421 gagcctggcc agggcgtacg ccgccgccgc gcagcgcggc gcgtcccggt ggttgaggag
     3481 caggacgagc gattcgaagg cccggcggtc cccggcgacg ccgagcagga ccgccgccaa
     3541 ctcccttgtc cacaagggct gtcccggcga gacgagcacc tcggcgagct cctcgaggtc
     3601 accggtcgcg accagccgct cgtactcctt cgagcccccc gcctcggcgc gtaaacgttc
     3661 cgtcaccgtc cgcaactcct catccatggc cgcgagccta accgccgatc cctcccggcg
     3721 acgtagatca caaacactga gggctggcgc gctcgttact cgccggttaa tctcatgtga
     3781 gcggggcaca ctccccgtag cacctccctt tcccgcggtg gcctggtgac gcagccactg
     3841 tgggcagctt gtcggttcgg tacttcaggt accgctgcac cacctcgtac gacgtggctc
     3901 cgggacagag cccgtcggct tctcaccggg cgtgcccttc gcgccgcccg gacacgacac
     3961 gcaccacttt ccgcacgccg tacggcagtt gccgcctgcg cgtgcgcccc tcagtcgtca
     4021 ctctcttcct ggagtccacg catggacgct cccttgaaca ccgtcgccgt catcggtctg
     4081 ggcacgatgg gcaccggcat cgccgaggtc ctggcccgcg cgggccgcga ggtcatcggc
     4141 atcgacatca gccaggcggc ggccacccgg gcggtgacgg ccctggagac ctccacggcc
     4201 cgcgcggtgc ggcgcgagcg cctcaccgat gaggagcggc gcggcatcct ggcccgcttc
     4261 cgcaccttca ccgatctgca ggccgcggcc gacgtcgacc tggtgatcga ggtcgtcccc
     4321 gagtcgtacg agatcaagca gcggatcgtg cgtgaactcg acgccgtggt ccgccccgag
     4381 acgatcatcg cgaccggcac caacgcgctg tccgtcaccc ggctcgccgc cgagtcggcg
     4441 cgccccgagc gcgtgctcgg cctgcacttc ttcaaccccg cgcccgcgat gaagctcgtc
     4501 gagatcgtct cgtccgtgct caccgcgccc gccgccgtcg ccgcggtcac cgagctcgcg
     4561 tacgccctgg gcaaggagcc cgtcgcggtc ggcgaccggc ccggcttcgt cgccgacggg
     4621 ctgctcttcg gctacctcaa ccaggccgcc gcgatgtacg aggcgaagta cgcctcccgc
     4681 gaggacatcg acgccgcgat gaagctgggc tgcggcctgc cgatgggccc gctcgcgctg
     4741 ctcgacctga tcggcgtgga caccgcccgc acggtcctcg acgccatgta cgccgagtcc
     4801 cacgaccgcc tgcacgcccc cgcgccggtc ctcaagcagc tcagcgaggc gggcctgacc
     4861 gggcgcaagt cggggcgcgg cttctacacg tacgccgagc cgggcagcac ggaggtggtg
     4921 cgcgacgcgc tgaccccgcc ggagggcgag cggagcgtcc cgagccgtgc cgtgcgcgcg
     4981 gtcggtgtgg cgggctccgg cacgatggcg tccggcatcg ccgaggtctt cgccaaggcg
     5041 ggctacgacg tggtgctcgc cgcacgcagc gaggagaagg cgcagctcgc cagggcccgt
     5101 atcggcaagt ccctcgcgcg ctcggtggag aaggggcgga tcaccgccga gtcggcggcg
     5161 gagaccctgg agcggatcac cccggccggt tcgtacgagg cgttcgccga tgtcgatctg
     5221 gccctggagg ccatcgcgga ggacctggac gtcaagcagc agctgttcgc gacgctcgac
     5281 aaggtctgca agcccggcgc gatcctggcg accacgacgt cctcgctgcc ggtcgtcgcc
     5341 tgcgcccgcg ccacctcgcg cccgcgtgac gtgatcggca tgcacttctt caacccggcc
     5401 ccggcgatga agctggtcga ggtggtgcgc accgtgctga cctcggacga ggtgcacgcc
     5461 accgtccgtg aggtctgcgc ggcgatccgc aagcacccgg tggactgcgg cgaccgcgcg
     5521 ggcttcatcg tgaacgcgct gctcttcccg tacctgaaca acgcgatcaa gatggtccag
     5581 gagcactacg ccacgctgga cgacatcgac gccgcgatga agctgggcgg cggctacccc
     5641 atggggccct tcgagctcct cgacgtggtc ggcctcgatg tctcgctcgc catcgagaag
     5701 gtcctgcacc gcgagttccg cgacccgggc ctggccccgg cgccgctgct cgaacacctg
     5761 gtggccgcgg gctgcctcgg ccgcaagacg gggcgtggct tccgcgaata tgcccggcgc
     5821 tgagccggcc gaggaccgcc tggactccgc gacggactgg ggcgggctgc tcgaaccctc
     5881 gggcggccct ctcccacacg agcaccgtgc tgcgcatatg cagtacgttc gtggcatgtc
     5941 caaggccgcc aagtcctcac gcaacgccag ccccgacgct ccggagagcg ccgcgggcag
     6001 ccgtgccgcc gcccaacgcc tcaagatgcg cagggagctg gctgcggcgg ccatggagct
     6061 cttcgcgacc aaggggtacg aggcgacgac ggtcgacgag atcgccgccg ccgcgggcgt
     6121 cgcccgccgg accttcttcc ggcacttccg ctccaaggaa gaggcgatct tccccgacca
     6181 cgacgacacc ctggtccgcg cggaggaggt gctgaacgcc gcaccggcgc acgagcaccc
     6241 gctcgacacg gtgtgccgcg gcatcaagga agtcatgaag atgtacgcgg cctcgcccgc
     6301 ggtctccgtg gagcgctacc ggctgacccg tgaggtgccc acgctgcgcg agcgcgagat
     6361 cgcctccgtg gcccgctacg aacggctgtt cacgcggtat ctgctcggcc acttcgatga
     6421 gcaggcgcac cacgacggca acgacgaccc gctgctcgcc gaggtcgccg cctccgcggt
     6481 ggtcaccgcc cacaaccacg tcctgcgccg ctggctgcgg gcgggcggcc agggcgacct
     6541 ggaggcccac ctgaaccacg cgttcggcat cgtccggctc accttcggca ccgggatcgg
     6601 cgcgggccgc tcctcggcac cggccaccgc ccccgccacg gtctccgcgc agggcgaggt
     6661 cctggtcacc gtggcccgta cggacgcgcc gctcgacgag gtcatgcgga cgatcgagaa
     6721 agccctccgc gagcgggcct catagtccgc ttttcgaccg attcgtcctt ggcttttcag
     6781 ggggcgctac ccgccggtag cgcccccttc ggcatgccgc taccccctct gacctgcact
     6841 gatcgatcat cggtcaaaaa ctcatggcac tcagtgcctt gccccttggc acctggtgtc
     6901 atacgttgat gttgtccggg cggccggcgt gcagagatca tccgtacgtc ggctgtcccc
     6961 tcaagccacg gcttgtacgc ccggacgcct gcgtcacagg cacctctcgc gccccaccac
     7021 ggcgccgccg agcaccaccc ttcgccgaac cgacggcaca cctccaccca gcaccacaag
     7081 caccccgacg tatccctcaa gacagcgctc ccgcagggcg cttcttcgcc ggaggcatca
     7141 ccgtgaagga aatcctggac gcgattcagt cgcaggacgc acagtccgcc gacttcgccg
     7201 ctctccccct gcccgactcg taccgcgcga tcaccgtgca caaggacgag acggagatgt
     7261 tcgcgggcct ggacacccgc gacaaggacc cccgcaagtc cctccacctg gacgacgtgc
     7321 cggtgcccga actcggcccc ggtgaagccc tggtggccgt gatggcctcc tcggtgaact
     7381 acaactccgt gtggacctcg atcttcgagc cgctgtcgac cttcggcttc ctggagcgct
     7441 acggcaaggt cagcgagctc accaagcgcc acgacctgcc gtaccacgtc atcggctccg
     7501 acctggcggg cgtcgtgctg cgcaccgggc ccggcgtgaa ctcctggaag ccgggcgacg
     7561 aggtcgtcgc gcactgcctg agcgtcgagt tggagtcgag cgacggccac aacgacacga
     7621 tgctcgaccc cgagcagcgc atctggggct tcgagacgaa cttcggcggc ctcgccgaga
     7681 tcgcgctcgt caagtccaac cagctcatgc ccaagccggg ccacctgagt tgggaggagg
     7741 ccgccgctcc cgggctcgtg aactccaccg cgtaccgcca gctcgtctcg cgcaacggcg
     7801 ccgacatgaa gcagggcgac aacgtgctga tctggggcgc gagcggcgga ctcggctcct
     7861 acgccacgca gttcgcgctc gccggcggcg ccaacccgat ctgtgtggtc tccagcgagc
     7921 agaaggccga catctgccgc tccatgggcg ccgaggcgat catcgaccgc agcgccgagg
     7981 gctaccggtt ctggaaggac gagcgcaccc aggaccccaa ggagtggaag cgcttcggca
     8041 agcgcatccg cgagttcacc ggcggcgagg acatcgacat cgtcttcgag caccccggcc
     8101 gcgagacctt cggcgccagc gtctacgtca cccgcaaggg cggcaccatc accacctgcg
     8161 cctccacctc gggctacatg cacgagtacg acaaccgcta cctgtggatg tccctcaagc
     8221 gcatcatcgg ctcccacttc gccaactacc gcgaggcctg ggaggccaac cgcctgatcg
     8281 ccaagggcaa gatccacccc accctctcca aggtctactc cctcgaagag acaggccagg
     8341 cggcccacga cgtccaccgc aacgcccacc agggcaaggt cggcgtgctc gccctcgcgc
     8401 ccaaggaggg cctgggcgtg cgcgacccgg agctgcgggc ccagcacatc gacgccatca
     8461 accgtttccg caacgtctga accgagctag gagcagcgga gcatgacaga gcgtcagaag
     8521 gaccggccgt ggctcatgcg gacgtacgcc ggtcactcca cggcggaggc gtccaacgag
     8581 ctgtaccggc gcaacctcgc caaggggcag acgggcctct cggtcgcgtt cgacctgccc
     8641 acgcagaccg gctacgaccc cgaccacatc ctcgcccgcg gcgaggtcgg ccgggtcggc
     8701 gtcccggtct cgcacctggg tgacatgcgg cggctgttcc aggacatccc cctggaccgg
     8761 atgaacacct cgatgacgat caacgccacc gccatgtggc ttctggcgct ctatcaggtg
     8821 gtcgcggagg agcagggcgc ggacatcacc cagctccagg ggacgacgca gaacgacatc
     8881 gtcaaggagt acctctcgcg cgggacgcac gtcttcccgc cgggcccctc cctgcggctg
     8941 accaccgaca tgatcacgta cacggtggcc cacatcccca agtggaaccc gatcaacatc
     9001 tgcagctacc acctccagga ggcgggggcc acaccggtcc aggagatcgc gtacgcgatg
     9061 tccaccgcca tcgcggtcct cgatgccgta cgcgactccg ggcaggtccc cgaggagaag
     9121 ttcggggacg tcgtggcccg catctccttc ttcgtgaacg cgggcgtccg cttcatcgag
     9181 gagatgtgca agatgcgggc gttcgggcgg atctgggacc agatcacgcg cgagcggtac
     9241 ggcatcgaga acccgaagca gcgccggttc cgctacggcg tgcaggtcaa ctccctgggc
     9301 ctgaccgagg cccagccgga gaacaacgtc cagcggatcg tgctcgaaat gctggccgtc
     9361 accctctcca aggacgcacg cgcgcgtgcc gtgcaactcc cggcctggaa cgaggcgttg
     9421 ggactgcccc gcccctggga ccagcagtgg tccctgcgca tccagcaggt cctcgcgcac
     9481 gagagcgacc tcctggagta cgaggacatc ttcgcgggct cgcacgtcat cgagaagaag
     9541 gtcgacgagc tggtcaccga ctcgctcacc gagatggacc ggatccagga gatgggcggc
     9601 gcgatggccg ccgtcgagtc cggctacctc aagtcccagc tcgtctcctc gcacgccgag
     9661 cgcagggcca ggatcgaggc gggccacgag aagatcgtcg gcgtgaacgt gtacgagtcg
     9721 accgagacca accctctcac cgccgacctg gacgccgcga tcatgacggt ggaccacgcc
     9781 gtcgaggcga aggtcgtgcg ggccatcggc gactggcgca ccacgcgtca ggagtcctcg
     9841 gaccgccagg gcatgggcga cccgttccac tacccgacca cggggcaggc cctggagcgc
     9901 ctcaaggagg cggccgcggg tacggagaac ctcatggagg ccaccctgga gtgcgcccgc
     9961 gccggggtca cgaccggcga gtggtcaggg gccctgcgcg aggtgttcgg cgagttccgg
    10021 gcccccaccg gagtctcgtc ggcgccggtc gcggtggccg cggaggaggg ctcgccgatg
    10081 gccgaggtcc gccgcaaggt cgccctgacc ggcgaggaac tcggcggcaa gctccgcctc
    10141 cttgtcggca agccgggcct ggacggccac tccaacggcg ccgagcagat cgccgtgcgc
    10201 gcccgcgacg ccgggttcga ggtggtctac cagggcatcc ggctcacccc ggaacagatc
    10261 gtgtccgccg cgctcgccga ggacgtgcac tgcgtcggcc tgtcgatcct ctccgggtca
    10321 cacgccgagc tggtgcccga cgtcctcgtc cggatgcgcg aggcgggcgc caccgacgtc
    10381 cccgtgatcg tcggtggcat catccccaac gccgacgccg aggacttgaa gcgtgcgggt
    10441 gtggccgccg tgttcacgcc caaggacttc ggcatcacgg agatcatcgg ccgtatcgtc
    10501 gacgagatcc ggaaagcgaa caagctcgac cctctggagg tccccgcatg accgcgccca
    10561 tcaaccgtct ccgtccccgc cgctcgtgtc tggcggtccc gggttcgaac ccgcgcttcc
    10621 tggagaaggc ccagggtctg cccgcggacc aggtcttcct ggacctggag gacgcctgcg
    10681 cgccgctcgc caagccggag gcccggcaca ccatcgtgaa gttcctcaac gagggcgact
    10741 ggacgggcaa gacgcgggtg gtgcgcgtca acgactggac caccgagtgg acgtaccgcg
    10801 acgtcgtcac ggtcgtcgag ggcgcgggcc agaacctcga ctgcatcatg ctgccgaagg
    10861 tgcagacggc cgaccaggtc gtcgcgctcg acctgctcct gacccagatc gagaagacca
    10921 tgggcttcga ggtcggcaag atcggcatcg aggcgcagat cgagaacgcc cagggcctca
    10981 acaacgtcaa cgcgatcgcc gaggcgtccc agcgcgtcga gacgatcatc ttcggcccgg
    11041 ccgacttcat ggcgtcgatc aacatgaagt ccctggtcgt cggcgagcag ccgcccgggt
    11101 acccggccga cgcctaccac ttcatcctga tgaagatcct gatggccgcc cgtgccaaca
    11161 acctccaggc gatcgacggc ccctacctcc agatcaagaa cgtcgacggc taccgcgagg
    11221 tcgcgaaccg cgccgcggcg ctcggcttcg acggcaagtg ggtcctgcac cccggccagg
    11281 tcgaggcggc caacgaggtc ttctcgccct cgcaggagga cttcgaccac gccgagctga
    11341 tcctggacgc gtacgagtac tacacgtccg aggcgggcgg aaagaagggc tcggcgatgc
    11401 tcggcgacga gatgatcgac gaggcgagcc ggaagatggc gctcgtcatc tccgggaagg
    11461 gccgtgcggc gggtatgcag cgcacgtcca cgttcgaggc cccggaggcg taagaccatg
    11521 cagttcggcc gcacctacga agagttcgaa gtcggcgccg tgtacaagca ctggcccgga
    11581 aaaacggtca ccgagtacga cgaccacctc ttctgtctcc tgaccatgaa ccaccacccc
    11641 ctccacatgg acaacaacta tgcggagaag acgacggact tcgggaagaa cgtcgtcgtg
    11701 ggcaattaca tctactcgct gctgctcggc atgtcggtcc ccgacgtctc cggcaaggcc
    11761 atcgccaacc tggaggtcga atcgctcaag cacgtggcgc cgaccttcca cggcgacacg
    11821 atctacggcg agacgacggt cctcgacaag acgccttcga agtccaagag cgaccgcggg
    11881 atcgtctacg tggagaccaa gggctacaag caggacggca cgctggtctg cgtgttccgc
    11941 cgcaaggtga tggtgcccac cgagacgtac atcaaggagc gcggcggcga gcagcccggc
    12001 cgcccggagc tcaagagcca ggagaagtag ccatgagccg actcgcccag accgcggggt
    12061 tgacggacat ccagcaggag atcctgtcca ccgtccgtga cttcgtcgac aaggagatca
    12121 ttccggtcgc gacggaactc gagcaccgtg acgagtaccc gcagcagatc gtggacggcc
    12181 tcaaggagtt gggcctgttc gggctcatga tccctgagga gtacggcggc ctcggcgagt
    12241 cgctgctcac ctacgcgctg tgcgtcgagg agatagcgcg cggctggatg tcggtgtccg
    12301 gcatcatcaa cacccacttc atcgtggcct acatgctcaa gcagcacggc acgcaggagc
    12361 agaaggagac gttcctgcct cgcatggcgg cgggcgaggt gcgcggcgcg ttctccatgt
    12421 cggagccggg actcggctca gatgtgtcgg ccatcacgtc gaagggcgtc aaggacgggg
    12481 acgactacgt cctcaacggc cagaagatgt ggttgacgaa cggcggcacg tccactttgg
    12541 tggccgtcct gtgccgaagt gacgaaggac accccgaggg cacggcgccc cacaagtcga
    12601 tgaccacgtt ccttgtcgag aaggagcccg gtttcggaga ggtccggccg ggcctcacca
    12661 tccccggcaa gatcgacaag atgggttaca agggcgtcga caccaccgaa ctcatcatgg
    12721 acgggctacg cattccggcc aatcgtgtgc tcggcggggc caccggccga gggttttacc
    12781 aaatgatgga cggcgtcgaa gtcggccgcg tcaatgtggc ggcgcgtggt tgcggcgtcg
    12841 cacagcgtgc atttgagctc ggtgtctctt atgcccagca acgccacact ttcggcaagc
    12901 cgatcgccca gcatcaggcg attcagttca agctggcgga gatggctacc aaggtcgagg
    12961 ccgcccatgc gatgatggtc aatgccgcac gcaaaaagga ctccggggag cgaaacgacc
    13021 tcgaagcagg gatggcgaag tacctcgcct ccgagtactg caaggaagtc gtggaggacg
    13081 cgttcaggat tcacggcggt tacggcttct cgaaggagta cgagatcgag cgcctctaca
    13141 gggaggctcc gatgctgttg atcggtgaag gtaccgccga gatccagaaa atgatcattg
    13201 gtcgtcgact gctcgaagag tatcgattcc agggctagat gcccggtttg gggcgttttc
    13261 ttcgcgaaga agatcacacc ctgtcaacac tcttcggccg tcgactcggc ttcctggctt
    13321 gcccagttgt ggctcgcaac cgatagcatc cggagaaagc cgccgtcccc cattcactgc
    13381 gcggcatcat ccgctacgaa ggtcatccat gccccacagc caaacctctg caccacgcga
    13441 cggcattctt cagggacgcc ttgctcgcgg agcatcgccg tggctcctgc cgaccgtcgc
    13501 caccgcggca ctcagcctcg cccgcgcgcg ccgctccaag ggcgccgcgg ccgtcgccgt
    13561 gcccgccacc gcgctggcgg cgggcatgct gtggttcttc cgcgaccccg agcgcgagat
    13621 cgcccagggc cgggtcatct ctcccgccga cggcgtggtg cagagcatca tgccgtggaa
    13681 ggacgggcgc acccgcgtcg ccatcttcat gagcccgctg aacgtccacg tcaaccgcgc
    13741 gcccctcgcg ggcacggtga cgtccgtgga gcacatcccc ggcgggttcg tcccggcgtt
    13801 caacaaggag agcgagaaca acgagcgcgt tgtctggcac ttcgacaccg agctcggcga
    13861 catcgagatg atccagatcg cgggcgcggt cgcgcgacgc atcgtgccct acgtgccgca
    13921 gggatcgaag gtggagcagg gcgagcggat cggcctgatc cgcttcggct cccgcgtcga
    13981 catctacctc cccgagggtg tcgaggtgga cgtcgaggtc ggtcagaaga ccgtggctgg
    14041 ggtgactcgc attgaccgtg gttgatcctg agacacaagc gggctgggtg cccgaggccg
    14101 cggaggacga ggccgacgag gagatgcctc tctcgctgcg cctctcaata gcggacaccc
    14161 tcacgctcgg taacgcgacg tgcgggttca tggcggtgta cttcaccacc acgggcatcc
    14221 tcatcccgca cctgcaaggc agcaacgaga gcggcatggc gcggcactcc gccgcgaccg
    14281 ccgtgatcct gatgctctgc gcggccgtct tcgacctgtt cgacgggctc gtggcgcgca
    14341 agctgcggtc ctcgccgatg ggcgccgagc tcgacaacct gtcggacctg atcagcttcg
    14401 gtctggcacc ggcgtacttc gtcctcgtct acggcatggt cgccgacgac gcgcaccagc
    14461 gggtggcggc ggtcggcgcg atcgtcgtgc tgttggcggt ggtgctgcgg cttgcgcggt
    14521 tctcgtgcgt gaccgtgaag gacggcacct tccaaggcat gccatcgccc ttcggcgcgc
    14581 tcacggtggt ctcgatcgtg ctcctggagc tgcccttcgt ggcgacgctc ctggcgatca
    14641 tcggcacggc ctggctcatg gtcagccgcg tcgagtaccc gaagccgcgc ggtcccctcg
    14701 cggtggccat gctctcgtgg atcgtcgcgg ccatggggct gctcgcggcc tgggcgttcg
    14761 acgctcccgg cgggcagctg ctcctgcaga cgggctgcgc gctccaggtc gtcctcggcg
    14821 cggtgatccc cctcttcgcc acggctcgac ggttgaacaa cttccgcgac aatcggcgcg
    14881 aggcgcgtgc ggcgcagttg ccttagcggc gcttgagcag tgtttgtggg gcccggaccg
    14941 attggtccgg gccccctttg cttgcgtctg tcgcccttcg ggctcgtcct caaactcccc
    15001 caagctctta aggagcaggg aggggccccc tcgacgggct attaat
//
