LOCUS       BGC0000715             18359 bp    DNA     linear   BCT 26-JUL-2016
DEFINITION  Streptomyces spectabilis spectinomycin biosynthesis gene cluster,
            complete sequence.
ACCESSION   BGC0000715
VERSION     BGC0000715
KEYWORDS    .
SOURCE      Streptomyces spectabilis
  ORGANISM  Streptomyces spectabilis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
COMMENT     On or before Nov 7, 2007 this sequence version replaced AF128272.1,
            AF145038.1, AF145039.1.
            ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 14:05:46
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             <1..744
                     /NRPS_PKS="Domain: TauD (1-237). E-value: 7e-48. Score:
                     155.5. Matches aSDomain: nrpspksdomains_ABW87795.1_TauD.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TauD"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1121:dioxygenase, TauD/TfdA (Score: 249.3; E-value:
                     7.2e-76)"
                     /gene_kind="biosynthetic-additional"
                     /product="unknown"
                     /protein_id="ABW87795.1"
                     /sec_met_domain="TauD (E-value: 2.1e-48, bitscore: 155.5,
                     seeds: 119, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLTDEQAAEVKRAFLRHHVLVFRDQVIDGEQHKRFARHFGELHPV
                     ALAPEGSDPHILEISADKDSRNVAGHGWHADGTADLKPSLGSMLYVTRTPEIGSGGDTM
                     FSNMHLAYEMLSPAMKELLDPMTAVHNGLLAWEGATPPPEYDVPVNVHPVVARHPDTGR
                     KLLFINGIYVSHIEQLSKGESRAIIDMLVKQITNTALLSCRVRWTPNTLVFWDNRCVQH
                     HAIWDYFPHSRYAQRVAIAGHRPTA"
     source          1..18359
                     /db_xref="ATCC:27741"
                     /db_xref="taxon:68270"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces spectabilis"
                     /strain="ATCC 27741"
     subregion       1..18359
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000715"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..18359
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="amglyccycl"
                     /region_number="1"
                     /rules="(DOIS or ValA or salQ or strH or strK1 or strK2 or
                     NeoL or SpcFG or SpcDK_glyc)"
                     /subregion_numbers="1"
                     /tool="antismash"
     aSDomain        4..711
                     /aSDomain="TauD"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ABW87795.1_TauD.1"
                     /evalue="7.00E-48"
                     /label="ABW87795.1_TauD.1"
                     /locus_tag="ABW87795.1"
                     /protein_end="237"
                     /protein_start="1"
                     /score="155.5"
                     /tool="antismash"
                     /translation="LTDEQAAEVKRAFLRHHVLVFRDQVIDGEQHKRFARHFGELHPVA
                     LAPEGSDPHILEISADKDSRNVAGHGWHADGTADLKPSLGSMLYVTRTPEIGSGGDTMF
                     SNMHLAYEMLSPAMKELLDPMTAVHNGLLAWEGATPPPEYDVPVNVHPVVARHPDTGRK
                     LLFINGIYVSHIEQLSKGESRAIIDMLVKQITNTALLSCRVRWTPNTLVFWDNRCVQHH
                     AIWDYFPHSRYAQR"
     stem_loop       745..798
     misc_feature    745..17984
                     /note="spectinomycin biosynthesis gene cluster"
     gene            complement(803..1606)
                     /gene="spcZ"
     CDS             complement(803..1606)
                     /codon_start=1
                     /gene="spcZ"
                     /note="hypothetical protein"
                     /product="SpcZ"
                     /protein_id="ABW87796.1"
                     /transl_table=11
                     /translation="MSRTPGAPEPAETTEAFERFVELLAAGPVGPEHPAAPGGPGLPSW
                     LAQLAAALFHGQDEQAARRWALRVHTALGRLGGPVPFDVVHDWHARAVAPLLAEVSARR
                     GGDGAAPDAVRRLHERALAGERATEAEWTEALGPALTQVYGHAYAYADAYATASVNARA
                     YAMDNDYGEEKAAEFAAMYAKLNTDANARSYADANALANTRALAAAFARADERAFAEAY
                     PFAYVHVYALADAERAEPAGRDERYRAACARLADGLAEALDRAAG"
     gene            complement(1685..2683)
                     /gene="spcN"
     CDS             complement(1685..2683)
                     /codon_start=1
                     /gene="spcN"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) APH"
                     /gene_kind="biosynthetic-additional"
                     /note="spectinomycin phosphstransferase"
                     /product="SpcN"
                     /protein_id="ABW87797.1"
                     /sec_met_domain="APH (E-value: 2.4e-18, bitscore: 57.1,
                     seeds: 156, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQNLPEKFDETRLWEALRGFGVSPSRVTYAPVGFGDYHWTVTDED
                     GRPWFATVSDLEHKEHCGQGAQAALKGLRQAMDTALALRDRDGLRFVVAPVAATDGGGP
                     VLPLDARYALTVFPHVPGRTGEFGQRLTEAERDRLLALLAELHGRTPPETTPPADMEPP
                     GLPGVRAALAESEGPWSGGPFAEPARLLLREHEATLHARLAEFEALVARVKGRGAPLVV
                     THGEPHPGNLILGEEGYLLVDWDTVGLAPAERDLSLISDDPADLARYAELTGRTPDPDA
                     LALYRLRWSLLDVAEFVEWFRAEHQRTQDTESAWKGFTDTLGQLAAGGSAA"
     protocluster    2054..18359
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[12053:17984]"
                     /cutoff="20000"
                     /detection_rule="(DOIS or ValA or salQ or strH or strK1 or
                     strK2 or NeoL or SpcFG or SpcDK_glyc)"
                     /neighbourhood="10000"
                     /product="amglyccycl"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      12054..17984
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(DOIS or ValA or salQ or strH or strK1 or
                     strK2 or NeoL or SpcFG or SpcDK_glyc)"
                     /neighbourhood="10000"
                     /product="amglyccycl"
                     /protocluster_number="1"
     cand_cluster    2054..18359
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(DOIS or ValA or salQ or strH or strK1 or
                     strK2 or NeoL or SpcFG or SpcDK_glyc)"
                     /kind="single"
                     /product="amglyccycl"
                     /protoclusters="1"
                     /tool="antismash"
     gene            complement(2693..3985)
                     /gene="spcT"
     CDS             complement(2693..3985)
                     /codon_start=1
                     /gene="spcT"
                     /gene_functions="transport (smcogs) SMCOG1020:major
                     facilitator transporter (Score: 329.9; E-value: 3.5e-100)"
                     /gene_kind="transport"
                     /note="putative spectinomycin exporter"
                     /product="SpcT"
                     /protein_id="ABW87798.1"
                     /transl_table=11
                     /translation="MESPASDALLPPGPDPGAERAARRGFRLLVGSHLLNETGAAATLV
                     VLPLTAVLALDASAWQTALIESAYFCAYLVLGLPAGALVERARPRRVMIGADAVRCVAL
                     ASLPLAWAADGLSLPMVYAVALLLGCAQLFGDIADHSYLPRLVREEQLVQGNSTLQLIR
                     SGAELGGPGLGGLSVQWLGAYWALAANAVGSAVSAVLLWRIKVPEPEPPALAHGLARQT
                     WEGLSFVRRQPVLRMLAVSASLNNLVFSAAFALDVVFLSKVVGVPAGLVGVLLASGAVG
                     ALAGAWLTPRLARRFGDARTATLAVPVAAPFLLLAPLTQDDWRVLLFALAHLAISVGVT
                     VFNILQVTYRQRVCPADLLSRVSAIFRFAVWGAAPLGALAGGALAAHLGVRPALWTLAV
                     ALLAVAPLLLCSPLRRTRDFDVNFPTVPTSC"
     gene            complement(3990..4784)
                     /gene="spcA"
     CDS             complement(3990..4784)
                     /codon_start=1
                     /gene="spcA"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1207:inositol monophosphatase (Score: 241; E-value:
                     3e-73)"
                     /gene_kind="biosynthetic-additional"
                     /note="inositol-1-monophosphatase"
                     /product="SpcA"
                     /protein_id="ABW87799.1"
                     /transl_table=11
                     /translation="MSLAHAEGGAALLRLAVRAAHRAGELLLEGAAAGPPEVRTKSSAK
                     DLVTAFDRGSEACVVEILTAARPDDGILGEEGGERPGGSGVRWVIDPLDGTANFVSGYP
                     AFVVSIAAERAGRTEVGVVYDPSRRETFTAIRGRGAARDGLPLTVSGAERLEQALVSTG
                     FSSDPATRDRQAALAARVVARVRDIRSSGSAALDLCWVAAGRLDAYYESGTRLWDRAAG
                     ALIAEEAGAWVGGADGRPPCDAMTIACAPKTADALRALVGHA"
     misc_feature    complement(4749..4751)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            5159..6283
                     /gene="spcB"
     CDS             5159..6283
                     /codon_start=1
                     /gene="spcB"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1079:oxidoreductase (Score: 203; E-value: 1.3e-61)"
                     /gene_kind="biosynthetic-additional"
                     /note="Myo-inositol dehydrogenase"
                     /product="SpcB"
                     /protein_id="ABW87800.1"
                     /transl_table=11
                     /translation="MQKERVRVGVIGCGTVAQIMHLPYLQSLSDQFEITALSDLSPGLL
                     DALGERYGVPHGRRFTDYRALLDTDVDAVLVLSGGSHAPQVLAAAEAGKHVLVEKPLCF
                     TLREADAIEAAVARSGVRLMVAYMKRFDPGFLYGKQLVSAIEEPRYAQINTLHPSEDQY
                     IAMHGVRRFDDVPGQVARELHRAQEELLDEAVGAVPDALRFVYHDVFLGSMVHDVNALR
                     SLVGEPDDVLFTEIWPRDSRFPSVTTVLAHPGDLRTSYTWTYLDDVRDYFEEIAVLSAG
                     QRVRIQFPSPFLKHWPTPVVAQHMEDGALVERRIQASYDEAFREELRAFHACVVDGTPP
                     LTDVRDAREDIAVLQRVFARSAPGGLGGEAGKCP"
     gene            6389..7717
                     /gene="spcS1"
     CDS             6389..7717
                     /NRPS_PKS="Domain: Aminotran_3 (35-374). E-value: 4.1e-91.
                     Score: 297.3. Matches aSDomain:
                     nrpspksdomains_spcS1_Aminotran_3.1"
                     /codon_start=1
                     /gene="spcS1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013:aminotransferase class-III (Score: 396.3;
                     E-value: 2.6e-120)"
                     /gene_kind="biosynthetic-additional"
                     /note="PLP-dependent sugar aminotransferase"
                     /product="SpcS1"
                     /protein_id="ABW87801.1"
                     /sec_met_domain="Aminotran_3 (E-value: 5.9e-98, bitscore:
                     318.2, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTTAMPASTHHTIALEQSHRLPGGQSMTDLSGLVVDRARNAEVW
                     DKDGKRYIDFFTGVGVCNIGHSHPRFLAEVGEQLSACAVGTFYTDARSRYYELLAAQLP
                     ERLGRIHMFSTGSEAVEAAVKLARAATGKHEVVSFWGGFHGKTQGALSLHGGPRKHRSG
                     PFPPGSHQVPYAYCYRCPLQLEHSTCGQRCVDLAEQSIENGSVGDIAAIIVEPVQGTNG
                     NIIPPAGYLRAVRRLADRHGALLILDEVITGFGRTGSLFAFEQDPEVRPDVVVLGKAMA
                     SGVPASAIVSRAELVEGTSFGQPSAAASTFGGNPLASAAALATLRILLDERLPERSRLL
                     GETVARRLASWKEEFPFVGNAANVGLMVGVELVEPGTRRPLPKDVTRRIFQGLLAEGVL
                     AMAYDSRIRIYPPLSIPADHLDEGLTAFESVFRSLRSLRSTQT"
     aSDomain        6494..7510
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_spcS1_Aminotran_3.1"
                     /evalue="4.10E-91"
                     /label="spcS1_Aminotran_3.1"
                     /locus_tag="spcS1"
                     /protein_end="374"
                     /protein_start="35"
                     /score="297.3"
                     /tool="antismash"
                     /translation="VDRARNAEVWDKDGKRYIDFFTGVGVCNIGHSHPRFLAEVGEQLS
                     ACAVGTFYTDARSRYYELLAAQLPERLGRIHMFSTGSEAVEAAVKLARAATGKHEVVSF
                     WGGFHGKTQGALSLHGGPRKHRSGPFPPGSHQVPYAYCYRCPLQLEHSTCGQRCVDLAE
                     QSIENGSVGDIAAIIVEPVQGTNGNIIPPAGYLRAVRRLADRHGALLILDEVITGFGRT
                     GSLFAFEQDPEVRPDVVVLGKAMASGVPASAIVSRAELVEGTSFGQPSAAASTFGGNPL
                     ASAAALATLRILLDERLPERSRLLGETVARRLASWKEEFPFVGNAANVGLMVGVELVE"
     gene            7759..8559
                     /gene="speD"
     CDS             7759..8559
                     /codon_start=1
                     /gene="speD"
                     /note="putative hydrolase"
                     /product="SpeD"
                     /protein_id="ABW87802.1"
                     /transl_table=11
                     /translation="MELGCVVRSAQEAELTARLPLDYLEIKGDMLCVEPGEFAALCVRL
                     RAAGLPFKAMTSPLPRRFKCRVVGPDADHAAALDVFRDMCDRGAVLGVRTVVLGSGQAR
                     SVPPGHSSGQALGQFRDFLVQAHQVCAERDMTLALEPLNRTETNFVNSCAEAREVVDGL
                     TGVDVRITADCFHIMSEQLPIADEVAAAGAAVGHAHTSSVPRGSGDFHEGAQKEFVAAL
                     LAAGHRGGLTVEDDFGDFGREAPAAVDVFRRVLASASASGPSLP"
     gene            8571..9761
                     /gene="speX"
     CDS             8571..9761
                     /codon_start=1
                     /gene="speX"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1143:sugar-binding lipoprotein (Score: 185.7; E-value:
                     2.9e-56)"
                     /gene_kind="biosynthetic-additional"
                     /note="extracellular solute and sugar binding protein"
                     /product="SpeX"
                     /protein_id="ABW87803.1"
                     /transl_table=11
                     /translation="MPLSFLTDHTSPLMRGFADEVVARWNERRPDCPVGLSVLDHEELR
                     DLLHAGLTGERPPDVMTWFAGNRMRSLVDQGLMLDISAMWRAEGFADVYLPRFRAMTGG
                     MGPSSFLPTSHYWWAVYYRPSVFAELGIRTPVETWQDLTDACAVLRRAGITPFSLGARH
                     RCPAAAWFDYLDMRLNGPAFHQDLMALRVPYTDPRVTKVFAFWQRLVEDGWFLGDPAAY
                     DEEEAVAAVLRGEAGMTLVGAYATDEYTPPGEPDIDFFRFPLIDPGLPIGEDTPVDGYF
                     VAGTSEAPKEATAFLAHLGSREVQQLTIERLATLPTRTDVDVARGGRHVTKGMEIVRNA
                     DHLGQFYDLDTTWELSDTGMAAFVSFLREPSRAVELLHEVEAARQELLAAPSSAPV"
     gene            9828..11147
                     /gene="spcS2"
     CDS             9828..11147
                     /NRPS_PKS="Domain: Aminotran_1_2 (36-160). E-value:
                     3.3e-08. Score: 24.9. Matches aSDomain:
                     nrpspksdomains_spcS2_Aminotran_1_2.1"
                     /codon_start=1
                     /gene="spcS2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DegT_DnrJ_EryC1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1056:DegT/DnrJ/EryC1/StrS aminotransferase (Score:
                     358.1; E-value: 9.6e-109)"
                     /gene_kind="biosynthetic-additional"
                     /note="L-glutamine; scyllo-inosose aminotransferase"
                     /product="SpcS2"
                     /protein_id="ABW87804.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 9.8e-09, bitscore:
                     24.9, seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="DegT_DnrJ_EryC1 (E-value: 6.6e-95,
                     bitscore: 308.5, seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSAVRSSPLALLGGTPVHDGAWPAWPQAPDSTLTLLNEAATSGRW
                     AVSGIYTGTPSFERRFSDAFAAYHAAPGVTPYAVPTTNGSAALTIAMEALGVGPGTEVI
                     VPGLTWVACASAAHALGATPVLVDVDPHTLSISADAAKAAVTDRTRAILVVHAYCSGAD
                     VDAFQELSQSTGIPVIEDCSQAHGAEWRGRKVGTFGTIGVFSLQQTKVLTCGEGGVVLT
                     SSPELHDRLQQYRANGRRYTARPVAGRLELEDVGAVEGHNHSMSEFHAAVALSQLEFLE
                     EQSALRERNAAVLTDLLAGIPGVSTLPAPAGLTRRTYYDYVIRLDAQLLGPVPVDRVVD
                     AMSAELNMFFETLDTPLNANPLYVPLKSPRTPGSDEARRGLDPGRFELPAATAAYRTCF
                     AFLHQALLGTERDVTAIATAVEKVVTQLDRLGDGAERGRA"
     aSDomain        9936..10307
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_spcS2_Aminotran_1_2.1"
                     /evalue="3.30E-08"
                     /label="spcS2_Aminotran_1_2.1"
                     /locus_tag="spcS2"
                     /protein_end="160"
                     /protein_start="36"
                     /score="24.9"
                     /tool="antismash"
                     /translation="NEAATSGRWAVSGIYTGTPSFERRFSDAFAAYHAAPGVTPYAVPT
                     TNGSAALTIAMEALGVGPGTEVIVPGLTWVACASAAHALGATPVLVDVDPHTLSISADA
                     AKAAVTDRTRAILVVHAYCS"
     gene            11144..12052
                     /gene="speY"
     CDS             11144..12052
                     /codon_start=1
                     /gene="speY"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_12"
                     /gene_kind="biosynthetic-additional"
                     /note="oxidoreductase"
                     /product="SpeY"
                     /protein_id="ABW87805.1"
                     /sec_met_domain="Fer4_12 (E-value: 3e-08, bitscore: 24.2,
                     seeds: 84, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSVRLADGKVRNPEGIEVNASLQCNMRCQSCAHLSPLYRRENADP
                     AEIHDTLSVLARSYHASYAKIMGGEPLLHPDVVGLIEAVRATGISDTVLVATNGTLLHR
                     ATERFWQAVDSLEISVYPSRMIAPEEIERYRVLAREHGVSLLVNYYGHFRAVYSESGTD
                     APDLVRDVFDTCKLAHFWNSHTVYDGWLYRCPQSVFMPRQLRDGGWDPRVDGLRIEDDP
                     AFLERLHRFLTADDPLRACRNCLGSVGKLHPHQELPRAGWQVTEQLEELVDYPFLKVCK
                     DDITADDGCVERSLSAPVGGA"
     gene            12054..12935
                     /gene="spcG"
     CDS             12054..12935
                     /codon_start=1
                     /gene="spcG"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     amglyccycl: SpcFG"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic"
                     /note="glycosyltransferase"
                     /product="SpcG"
                     /protein_id="ABW87806.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 2.4e-13,
                     bitscore: 40.5, seeds: 256, tool: rule-based-clusters)"
                     /sec_met_domain="SpcFG (E-value: 1.7e-116, bitscore: 377.7,
                     seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTGNEKPAAAGPGESVTVVTLTRYRPVLVQRAMRSVRAQVADVPV
                     RHLVLIDDSEETLTALSEVTDAFPHCEVRYVPREPHEKSGPGRSSRLRNLGVRLSDSSW
                     IAYLDDDNEWTPDHLQSLLDQARSVRAVYSEVGLLDKDGAPYLEPRWPWANSVAEAERM
                     YADYVAKGICVPGSNVIKDRPGTYEVPVDTSAWLLARELLLAVPFEEQFSAADADGLVS
                     EDDKLYYRLVQGREPMVCTGRDAPLLPGRLLQQPRDRHARGAGRDRLGLGVRRLPDTRG
                     ARRRQTRERVPW"
     gene            12932..13735
                     /gene="spcM"
     CDS             12932..13735
                     /codon_start=1
                     /gene="spcM"
                     /note="N-methyltransferase"
                     /product="SpcM"
                     /protein_id="ABW87807.1"
                     /transl_table=11
                     /translation="MTESTTAAGDRRALGAEHGSYHWGDSLALLEGVPDGSVRAVVCSP
                     PFEGPQLIADEDRAGACSSDWLMPFFGQFERVLRPDGCVAFELGGLWLSDAPGKAVQHA
                     GAVHALAKAGWRLVQDFYYYNPQLLNPEPEGAARAPDSVTPIWVMARTHDVHYDVSALR
                     HPARAPFVRGNLLEFDTSGVYDRAYEKTLAETSLHPYVDRWPTVVPELFVELLTRPGDL
                     VLDPFAGSGATCFAAERLGRRWLGFELDRGLEQHVRAMFAARRAG"
     gene            complement(13801..14838)
                     /gene="speH"
     CDS             complement(13801..14838)
                     /codon_start=1
                     /gene="speH"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1079:oxidoreductase (Score: 166.9; E-value: 1.3e-50)"
                     /gene_kind="biosynthetic-additional"
                     /note="NDP-glucose dehydrogenase"
                     /product="SpeH"
                     /protein_id="ABW87808.1"
                     /transl_table=11
                     /translation="MRGAIIGFGTIAMGHMVGYSRVEDLSIAADVDVSKERRSMPEESF
                     GLPAYSDFAKLVANETLDFVDICTPPSSHAEYSDLALAHGLHVLCEKPVFLPAADGYAG
                     QLGRIHASDRVYYPCHVYKYAPVLAAMKERIAAPGFGKVVGAHFRTLRAGHAKGVPDWR
                     PHWRREPGTSHGGILRDHGPHSVYLAMNLTGLTPVSVSCLTGRLRDGAAYADTEDTALL
                     RLRCEDDVEIALHLSWAAGHRSTGYSIMGTSGSVVVDGDDLTYAADGVVVRTSIESGFD
                     DPSHQEWFQRMLLDFTAVVAEPERGRDLIREALTTSFVIDGAYASADGGRWVDCRVPEA
                     LLAPA"
     misc_feature    complement(14764..14766)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            15059..15988
                     /gene="speI"
     CDS             15059..15988
                     /codon_start=1
                     /gene="speI"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010:NAD-dependent epimerase/dehydratase (Score:
                     169.4; E-value: 2.1e-51)"
                     /gene_kind="biosynthetic-additional"
                     /note="NDP-glucose epimerase/dehydratase"
                     /product="SpeI"
                     /protein_id="ABW87809.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 7.8e-17, bitscore:
                     51.4, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLSPAGAAGSPAAARVAVVGGSGFIGRNLRKALDAAGVRTCSFT
                     RQRPFVQGGRVHRALLRSHTVFYVAGGVTPARAEREPRLAAQDYWNLQLLLWGLRGAAH
                     RPLVVLAGSGGTVYDPEADPPYREDAPTRPVSAYGAVKLAQERALAGAAWTAPVVLRLA
                     NVYGPGQSAGAGFGVIPHWAAAVRTGRALTLIGRSRRDYVHVDDVCAALLAVHRRADLL
                     RAARGPTTLNIGSGVPTSLDELHRHFERAAGRALDVRREPARSFDRSDVWLDVAAAREV
                     LGWTPRTGLADGLAGTLAAVAAPARLPS"
     gene            complement(16018..17010)
                     /gene="spcE"
     CDS             complement(16018..17010)
                     /NRPS_PKS="Domain: NAD_binding_4 (4-179). E-value: 2.7e-16.
                     Score: 51.2. Matches aSDomain:
                     nrpspksdomains_spcE_NAD_binding_4.1"
                     /codon_start=1
                     /gene="spcE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NAD_binding_4"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010:NAD-dependent epimerase/dehydratase (Score:
                     330.8; E-value: 1.4e-100)"
                     /gene_kind="biosynthetic-additional"
                     /note="dTDP-glucose 4,6 dehydratase"
                     /product="SpcE"
                     /protein_id="ABW87810.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 2.8e-20, bitscore:
                     62.7, seeds: 37, tool: rule-based-clusters)"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 3e-23, bitscore:
                     72.5, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="NAD_binding_4 (E-value: 8.3e-17, bitscore:
                     51.2, seeds: 102, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRIIVTGGAGFIGSEFVRSMLLAPGAANDIRITVLDKLTYAGVRE
                     NLDPVAGHPRHAFVHGDICDGDFLDQVVPGHDAVVHFAAETHVDRSITGSAPFVTTNVL
                     GTQLLLDAARRHGVGRFLHVSTDEVYGSIPVGSWSEESPLAPNSPYAATKAGSELLALA
                     CHRTHGMDVVVTRCSNNYGPYQFPEKVIPLFITRLLDGGKVPLYGDGQHVRDWLHVSDH
                     CRGVELALRHGRAGEVYHIGGGTELTNEQLTGHLLAATGRGWEQVERVPDRKGHDARYS
                     LDCTKAATELGYAPLVGFERGLADTVAWYAGNRSWWEPLQRRAALRASA"
     aSDomain        complement(16474..16998)
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_spcE_NAD_binding_4.1"
                     /evalue="2.70E-16"
                     /label="spcE_NAD_binding_4.1"
                     /locus_tag="spcE"
                     /protein_end="179"
                     /protein_start="4"
                     /score="51.2"
                     /tool="antismash"
                     /translation="VTGGAGFIGSEFVRSMLLAPGAANDIRITVLDKLTYAGVRENLDP
                     VAGHPRHAFVHGDICDGDFLDQVVPGHDAVVHFAAETHVDRSITGSAPFVTTNVLGTQL
                     LLDAARRHGVGRFLHVSTDEVYGSIPVGSWSEESPLAPNSPYAATKAGSELLALACHRT
                     HGMDVVVTRCSN"
     gene            17109..17984
                     /gene="spcD"
     CDS             17109..17984
                     /codon_start=1
                     /gene="spcD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     amglyccycl: SpcDK_glyc"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NTP_transf_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1064:glucose-1-phosphate
                     adenylyl/thymidylyltransferase (Score: 261.8; E-value:
                     1.2e-79)"
                     /gene_kind="biosynthetic"
                     /note="dTDP-glucose synthase"
                     /product="SpcD"
                     /protein_id="ABW87811.1"
                     /sec_met_domain="SpcDK_glyc (E-value: 2e-195, bitscore:
                     637.4, seeds: 2, tool: rule-based-clusters)"
                     /sec_met_domain="NTP_transf_3 (E-value: 5.4e-14, bitscore:
                     43.0, seeds: 297, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRGIILAGGTGSRLWPVTQAVSKQLMPVFDKPMVYYPLSTLMLAG
                     IRDILVITTREDEIDFRRLLGDGSHLGLRLTYRTQDRPGGIAEAFLIGADFIGGEPVAL
                     ILGDNIFHGRSFGVHVREHTAPDGGRIFAYPVADPTAYGVVEIGEDGTALSIEEKPAKP
                     KSRYAAVGLYFYDEHVVDIAAGLRPSERGELEITGVNEEYLRRGALSVTVLDRGTAWLD
                     TGTFTAMVQASEYVRVIEERQGLKIGCVEEVAWRCGYIDDERLRALSGPLLKSGYGRYL
                     LSLLDEEALL"
ORIGIN
        1 gagctcacgg acgagcaggc cgccgaggtc aagcgggcgt tcctccgcca ccacgtgctc
       61 gtcttccgcg accaggtgat cgacggcgag cagcacaagc gcttcgcccg gcacttcggc
      121 gagctgcacc cggtggcgct cgcccccgag ggctcggacc cgcacatcct ggagatcagc
      181 gccgacaagg actcccggaa cgtcgcgggc cacggctggc acgccgacgg caccgccgac
      241 ctgaagccgt cgctcggctc gatgctgtac gtcacgcgca ccccggagat cggcagcggc
      301 ggcgacacca tgttctccaa catgcacctc gcctacgaga tgctgtcgcc cgcgatgaag
      361 gaactcctgg acccgatgac cgcggtccac aacggcctgc tcgcctggga gggcgccacg
      421 ccgccgcccg agtacgacgt gccggtgaac gtgcaccccg tggtggcccg ccaccccgac
      481 acgggccgca agctgctctt catcaacggc atctacgtct cgcacatcga gcagctctcc
      541 aagggcgaga gccgggcgat catcgacatg ctcgtcaagc agatcaccaa caccgcgctg
      601 ctgagctgcc gcgtccgctg gaccccgaac acgctggtgt tctgggacaa ccgctgtgtg
      661 cagcaccacg cgatctggga ctacttcccg cactcgcgct acgcccagcg cgtggccatc
      721 gcgggccacc ggcccacggc ctgacgtacg cgagtggggg tggaggccgc gacggcctcc
      781 acccccactc gcgtacgcgg gctcacccgg cggcgcggtc cagggcctcg gcgagcccgt
      841 ccgcgagccg cgcgcacgcc gcccggtacc gctcgtcccg cccggccggc tccgcgcgct
      901 cggcgtcggc gagcgcgtag acgtggacgt acgcgaacgg atacgcctcg gcgaacgccc
      961 gctcgtcggc acgggcgaag gcggccgcca gcgcgcgcgt gttcgccagg gcgttggcgt
     1021 cggcgtagga gcgggcgttg gcgtcggtgt tgagcttcgc gtacatggcg gcgaactcgg
     1081 cggccttctc ctcgccgtag tcgttgtcca tcgcgtacgc gcgcgcgttg acggaggccg
     1141 tggcgtaggc gtcggcgtac gcgtaggcgt ggccgtagac ctgggtgagg gcggggccga
     1201 gggcctcggt ccactccgcc tccgtcgccc gctcccccgc cagggcccgc tcgtgcaggc
     1261 gccgtacggc gtccggggcg gcgccgtcgc cgccgcgccg ggcgctcacc tccgcgagga
     1321 gcggcgccac ggcgcgggcg tgccagtcgt gcacgacgtc gaacggcacg ggtccgccga
     1381 ggcggccgag cgcggtgtgc acccgcaggg cccagcgccg cgccgcctgc tcgtcctggc
     1441 cgtggaacag ggccgcggcc agctgcgcca gccacgacgg aagaccgggc ccgcccgggg
     1501 ccgccgggtg ctccggcccg accggaccgg ccgcgagcag ctcgacgaac cgctcgaacg
     1561 cctccgtcgt ctccgccggt tccggggcgc ccggggtgcg actcatctgc ctgtctcctg
     1621 tttcgtccgt tctcacggct cgaacaccgg ccgcccgtac accttacgca tcgggccgac
     1681 cgggctaggc tgcggatccg cccgccgcca gttgccccag cgtgtccgtg aagcccttcc
     1741 acgcggactc ggtgtcctgc gtgcgctggt gctcggcgcg gaaccactcc acgaactcgg
     1801 ccacgtccag caggctccag cgcagccggt agagcgcgag ggcgtccggg tccggggtgc
     1861 ggccggtcag ctcggcgtag cgggcgaggt ccgccgggtc gtccgagatc agggagaggt
     1921 cgcgctcggc cggggcgagg cccaccgtgt cccagtccac caggagatag ccctcctcac
     1981 ccaggatgag gttgcccgga tgcggctcgc cgtgcgtgac gacgagcggc gcgccccggc
     2041 ccttcacgcg cgcgacgagg gcctcgaact cggcgaggcg cgcgtggagc gtcgcctcgt
     2101 gctcgcgcag cagcagccgc gcgggctcgg cgaacgggcc gcccgaccag ggcccttccg
     2161 actcggccag ggccgcgcgc acgccgggaa ggcccggggg ctccatgtcg gcgggcggtg
     2221 tggtctccgg gggcgtccgg ccgtgcagct ccgcgagcag ggcgagcagc cggtcccgct
     2281 cggcctccgt caggcgctgc ccgaactccc ctgtgcggcc cgggacatgg gggaacaccg
     2341 tgagcgcgta ccgggcgtcc aggggcagta ctggaccgcc gccgtcggtg gcggccaccg
     2401 gtgccacgac gaaccgcagt ccgtcacggt cgcgcagggc cagcgccgtg tccatggcct
     2461 gtcggagccc cttcagcgcg gcctgtgccc cctgcccgca gtgctccttg tgctccaggt
     2521 cggagacggt ggcgaaccac ggccggccgt cctcgtcggt caccgtccag tggtagtccc
     2581 cgaatccgac gggggcgtag gtgacacgcg acggggacac accaaatccc ctcaaagctt
     2641 cccataggcg tgtttcatcg aacttttcag gaagattctg catagctcca cgctagcaac
     2701 tagtgggcac ggtggggaag ttgacgtcga agtcccgcgt ccggcgcagc ggggagcaga
     2761 gcagcagcgg cgcgacggcg agcagggcga cggcgagcgt ccacagcgcg gggcggaccc
     2821 cgaggtgtgc cgcgagggcg ccgcccgcga gggcccccag gggcgcggca ccccacaccg
     2881 cgaagcggaa gatcgccgac acgcggctga gcaggtccgc cgggcacacc cgctgccggt
     2941 aggtgacctg gaggatgttg aagacggtga cgccgacgct gatggcgagg tgggcgaggg
     3001 cgaagagcag cacccgccag tcgtcctggg tcagcggcgc gagcagcagg aacggggcgg
     3061 cgacgggcac cgcgagcgtc gccgtgcggg cgtcgccgaa gcggcgggcg agccgcggcg
     3121 tcagccaggc cccggcgagc gcgcccaccg ctcccgacgc gaggagcact ccgacgagcc
     3181 cggccgggac gccgacgacc ttgctcagga acaccacgtc gagcgcgaag gcggcggaga
     3241 agacgaggtt gttcagactg gcggagaccg cgagcatgcg caggaccggc tggcggcgca
     3301 cgaaggaaag accttcccag gtctgccggg ccaggccgtg cgcgagcgcg ggcggctcgg
     3361 gctcgggcac cttgatccgc cacaggagca ccgcggagac ggccgagccc acggcgttcg
     3421 ccgcgagcgc ccagtacgcg ccgagccact gcacggagag gccgccgagt cccggcccgc
     3481 ccaactcggc gccggacctg atgagttgga gcgtgctgtt gccctgcacg agctgctcct
     3541 cgcggaccag gcgcggcaga tagctgtggt cggcgatgtc gccgaacagc tgcgcgcagc
     3601 cgagcagcag cgccaccgcg tagaccatcg gcagggagag gccgtcggcc gcccaggcga
     3661 gcggcagcga ggcgagcgcc acacagcgga ccgcgtcggc gccgatcatg acgcggcgcg
     3721 ggcgcgcccg ctcgaccagt gcgcccgcgg ggaggccgag caccaagtag gcacagaagt
     3781 aggcgctttc gatcagggcg gtctgccagg ccgaggcgtc cagggcgagc accgcggtca
     3841 gcggcaggac gacgagcgtc gccgccgccc cggtctcgtt gagcaggtgc gagccgacga
     3901 gcagccggaa tccgcgccgc gcggcccgct cggcgcccgg gtccgggccc ggtggcagca
     3961 gggcgtcgga ggccgggctc tccaccgggt caggcatggc ccaccagggc gcgcagcgcg
     4021 tccgccgtct tcggggcgca cgcgatcgtc atcgcgtcgc agggcggccg cccgtccgcg
     4081 ccgccgaccc aggccccggc ctcctcggcg atcagcgctc cggccgcgcg gtcccacagg
     4141 cgcgtaccgg attcgtagta ggcgtcgagg cggccggccg cgacccagca caggtcgagg
     4201 gcggcggagc cgctgctgcg gatgtcccgc acgcgggcca ccacgcgggc ggcgagggcc
     4261 gcctggcggt cgcgggtcgc cgggtcgctg ctgaatcccg tggagaccag ggcctgttcg
     4321 agccgctcgg cgccggacac ggtcagcggc aggccgtcac gggcggctcc ccggccgcgg
     4381 atcgcggtga acgtctcccg gcgggagggg tcgtacacga cccccacctc ggtgcgcccc
     4441 gcgcgctcgg cggcgatgga cacgacgaac gccggatacc cgctgacgaa gttcgccgtg
     4501 ccgtccaggg ggtcgatcac ccagcgcacg ccggagccgc ccgggcgctc gccgccctcc
     4561 tcccccagga tgccgtcgtc gggccgggcc gcggtgagga tctcgacgac gcacgcctcg
     4621 ctcccgcggt cgaaggcagt caccaggtcc ttggccgacg acttggtccg tacctcgggc
     4681 ggccccgccg cggccccttc gagcagcagc tcaccggccc ggtgtgcggc cctgacggcg
     4741 agtcggagta acgcggcacc gccctcggca tgtgccaaag acatgggcaa cccccttgtg
     4801 gtgtacacgc gattatgtaa aggtaccacc aagtcgttcc aagggtgtga gccgcgctga
     4861 atcttctcgg cgcgcgggaa ctcgcccgcc gtgtatgtgc cttggggccg ttccaagcac
     4921 gggggatttg agtcaggttc agtaagccga acgatgtcgg ggcccagagg agacctttgt
     4981 ggaattccgc ccaactgcat cggtgacgta ggtccttcgg cgtcatcgtc cgttttccgt
     5041 cgggcgcggt gcgccgatgg cttcacccct ttcgggaaag caggtccggc ctcttcgggc
     5101 tgccctgatt tccccgctcc ggtccgccgc tccactcctt tcgagagaag ttggtgccgt
     5161 gcagaaagaa cgtgtccgcg tgggcgtcat cggttgcgga accgtcgccc agatcatgca
     5221 tctgccctat ctgcagagcc tctccgacca gttcgagatc accgcgctca gcgacctctc
     5281 ccccggcctc ctcgacgccc tcggcgagcg gtacggggtg ccgcacgggc ggcgcttcac
     5341 ggactaccgc gcgctgctcg acaccgatgt ggacgctgtc ctcgtgctca gcggcggcag
     5401 tcacgcgccg caggtcctgg ccgccgccga ggcgggcaaa cacgtcctgg tggagaagcc
     5461 gctgtgcttc acgctccgcg aggccgacgc gatcgaggcg gccgtcgccc ggtccggagt
     5521 gcgcctcatg gtggcctaca tgaagcgctt cgaccccggc ttcctctatg ggaaacagct
     5581 ggtcagcgcc atcgaggagc ctcggtacgc gcagatcaac accctgcacc ccagcgagga
     5641 ccagtacatc gccatgcacg gcgtgcgccg cttcgacgac gtgccgggcc aggtcgcgcg
     5701 ggagctgcac cgggcccagg aggagctgct cgacgaggcg gtcggcgcgg tcccggacgc
     5761 cctgcgcttc gtctaccacg acgtgttcct cggcagcatg gtgcacgacg tgaacgcgct
     5821 gcggtccctt gtgggagagc cggacgacgt gctgttcacg gagatctggc cgcgggactc
     5881 ccgcttcccc tccgtgacga cggtcctcgc gcacccgggc gacctgcgga ccagctacac
     5941 ctggacctat ctcgacgacg tccgcgacta cttcgaagag atcgcggtgc tttccgcagg
     6001 gcagcgggtg cgcattcagt tcccctcccc attcctcaag cactggccca ctcctgtcgt
     6061 cgcccagcac atggaggacg gcgccctggt ggaaaggaga attcaggcct cgtacgacga
     6121 ggcgttccgc gaggaactgc gcgctttcca tgcctgcgtc gtggacggca cgccaccgct
     6181 caccgatgtg cgcgacgctc gggaggacat cgccgtactc cagcgcgtct tcgccaggag
     6241 cgcacccgga ggactcggcg gcgaagcggg caaatgcccg tgatccaggg cctctcccca
     6301 ccacgccgaa cttcaaagga atgtaaagca taaacgcgag aggcaatgcg aagccgacgc
     6361 acaacgcggc agaaaggccc agggaagcat gagcaccacc gccatgcccg cgtcgaccca
     6421 ccacaccatc gcccttgagc agagccaccg cctgccgggc gggcagtcca tgacggacct
     6481 gtccggtctc gtcgtggacc gcgcgcgcaa cgccgaggtg tgggacaagg acggcaagcg
     6541 gtacatcgac ttcttcaccg gcgtgggcgt gtgcaacatc gggcactccc accccaggtt
     6601 cctggccgag gtcggggagc agctcagcgc gtgcgccgtc ggcacgttct acaccgacgc
     6661 gcgctcgcgt tactacgagc tcctcgccgc ccaactcccc gagcggctgg gccggataca
     6721 catgttctcg accggcagcg aggcggtcga ggccgccgtg aagctcgccc gggccgccac
     6781 cggcaagcac gaggtcgtca gcttctgggg cggcttccac ggcaagaccc agggcgccct
     6841 cagcctgcac ggcgggcccc gcaagcaccg ctcgggaccg ttcccgccgg gctcccacca
     6901 ggtgccgtac gcctactgct accgctgccc cctccagctg gagcacagca cctgcggcca
     6961 gcggtgcgtg gacctcgccg agcagtccat cgagaacggc tcggtcgggg acatcgccgc
     7021 gatcatcgtg gagccggtgc agggcacgaa cggcaacatc atcccgcccg ccggatatct
     7081 gcgggccgtg cggcggctcg cggaccggca cggggccctg ctcatcctgg acgaggtcat
     7141 caccggcttc ggcaggaccg gctccctgtt cgccttcgag caggatcccg aggtccgccc
     7201 ggacgtcgtc gtgctcggca aggcgatggc gtcgggcgtg cccgcgagcg cgatcgtcag
     7261 ccgtgcggag ctggtcgagg gcacctcctt cggccagccg agcgcggccg cctccacctt
     7321 cggcggcaac ccgctggcca gcgccgccgc gctcgccacc ctgcggatcc tgctcgacga
     7381 gcggctgccc gagcggtccc gtctgctcgg ggagacggtg gcgcgccgac tggcctcctg
     7441 gaaggaggag ttcccgttcg tgggcaacgc cgcgaacgtg ggcctcatgg tcggcgtgga
     7501 gctcgtcgag cccggcaccc ggcggccgct gcccaaggac gtcacccgcc ggatcttcca
     7561 gggcctcctc gccgagggcg tcctggcgat ggcgtacgac agcaggatcc ggatctaccc
     7621 cccgctgagc atcccggcgg accacctcga cgaggggctc acggccttcg agtcggtctt
     7681 ccggtcactg cggtccctcc ggtcgacgca gacgtgacgc ggcgacgacg cagaagtgac
     7741 acagaaatga ggagcgccat ggaactcggg tgcgtggtgc gcagcgccca ggaggccgag
     7801 ctgaccgccc gcctcccgct ggactacctg gagatcaaag gagacatgct gtgtgtcgaa
     7861 cccggtgagt tcgccgccct gtgcgtccgg ctgcgggcgg cgggcctgcc cttcaaggcc
     7921 atgacgtccc cgctgccgcg ccgcttcaag tgccgtgtcg tgggcccgga cgccgaccac
     7981 gcggcggcac tggacgtgtt ccgcgacatg tgcgaccggg gggccgtgct cggcgtgcgc
     8041 acggtcgtgc tcggcagcgg gcaggcccgc tccgtgccgc ccggacactc gtccgggcag
     8101 gccctcgggc agttcaggga cttcctggtc caggcgcacc aggtgtgtgc cgagcgcgac
     8161 atgacgctcg ccctcgaacc cctgaaccgt acggagacca acttcgtgaa ctcctgcgcc
     8221 gaggcgcggg aggtcgtcga cggcctcacc ggcgtggacg tgcggatcac cgccgactgc
     8281 ttccacatca tgagcgaaca gctgccgatc gccgacgagg tggccgccgc gggagcggcc
     8341 gtcgggcacg cgcacacctc gtccgtgccg cgcggcagcg gtgacttcca cgagggcgcg
     8401 cagaaggagt tcgtggcggc cctgctcgcc gcggggcacc ggggcgggct caccgtcgag
     8461 gacgacttcg gcgacttcgg gcgcgaggcg cccgccgcgg tcgacgtctt ccgccgcgtc
     8521 ctcgcctccg cttccgcctc cggcccttcc ctgccctgag gagtccgtac atgcccctga
     8581 gcttcctcac cgaccacacc tcgcccctga tgcgcggctt cgcggacgag gtcgtcgccc
     8641 gctggaacga acggcgcccg gactgcccgg tcggcctgtc cgtcctggac cacgaggagc
     8701 tgcgcgacct tctgcacgcc ggactcaccg gcgaaaggcc gccggacgtc atgacctggt
     8761 tcgccggcaa ccgcatgcgc tccctggtgg accagggcct gatgctcgac atctccgcga
     8821 tgtggcgggc ggagggcttc gccgatgtgt acctgccgag gttccgcgcc atgaccggcg
     8881 gcatgggccc ctcgtccttc ctgccgacct cccactactg gtgggccgtg tactaccgcc
     8941 cttcggtctt cgccgagctc ggcatccgca cgccggtcga gacctggcag gacctcacgg
     9001 acgcgtgcgc cgtgctgcgg cgggcgggca tcaccccgtt ctccctcggc gcgcgccacc
     9061 gctgcccggc cgccgcctgg ttcgactacc tcgacatgcg gctcaacggc cccgcgttcc
     9121 accaggatct gatggccctg cgcgtgccgt acacggaccc ccgcgtgacc aaggtgttcg
     9181 cgttctggca gcgcctcgtg gaggacggct ggttcctcgg cgacccggcg gcgtacgacg
     9241 aggaggaggc cgtcgcggcg gtgctgcgcg gcgaggcggg catgacgctc gtgggcgcct
     9301 acgccaccga cgagtacacc ccgcccggcg agccggacat cgacttcttc cgcttccccc
     9361 tgatcgaccc cggcctgccc atcggcgagg acaccccggt cgacggctac ttcgtggccg
     9421 ggaccagtga ggcgccgaag gaggccaccg cgttcctcgc ccacctcggg tcgcgcgagg
     9481 tccagcagct gacgatcgag cggctcgcca cgctgcccac ccgcaccgac gtggacgtgg
     9541 cccggggcgg acggcacgtc accaagggca tggagatcgt ccggaacgcc gaccacctgg
     9601 ggcagttcta cgacctcgac accacctggg agctctcgga caccggcatg gcggcgttcg
     9661 tgtcgttcct gcgggagccg tcgcgcgccg tggagctgct gcacgaggtg gaggcggccc
     9721 gccaggagct cctcgcggcc ccgtcctcgg caccggtctg acccgccggt ccgccgcccc
     9781 tcctccttcc tccgcacacc cccgttcctg acgacaaggg gacagacatg tcagcggtac
     9841 gcagttcacc tctcgccctc ctcggcggca ccccggtcca cgacggcgcc tggccggcct
     9901 ggccgcaggc gcccgattcc accctgaccc tgctcaacga ggccgccacg tccgggcgtt
     9961 gggccgtcag cggcatctac accggcaccc cgagcttcga gcggcgcttc tccgacgcct
    10021 tcgccgcgta ccacgcggcg cccggcgtga cgccctacgc ggtgccgacg acgaacggct
    10081 cggccgcgct caccatcgcc atggaggccc tcggcgtcgg ccccggcacg gaggtcatcg
    10141 tgccgggtct gacctgggtg gcctgcgcgt ccgccgcaca cgcgctcggc gccacgccgg
    10201 tgctcgtgga cgtggacccg cacaccctga gcatctcggc cgacgccgcc aaggccgcgg
    10261 tcaccgaccg cacccgggcg atcctcgtcg tgcacgccta ctgctcgggc gccgacgtcg
    10321 acgcgttcca ggaactctcg cagagcaccg gcatccccgt catcgaggac tgctcgcagg
    10381 cccacggggc ggagtggcgg ggccgcaagg tgggcacgtt cggcaccatc ggggtgttca
    10441 gcctccagca gaccaaggtg ctcacctgcg gcgagggcgg cgtcgtcctc acctcgtcgc
    10501 ccgagctgca cgaccggctc cagcagtacc gcgccaacgg acggcgctac accgcccggc
    10561 cggtcgcggg ccggctggag ctggaggacg tcggcgcggt cgagggccac aaccactcca
    10621 tgtccgagtt ccacgcggcg gtcgccctca gccagctgga gttcctggag gagcagagcg
    10681 cactgcgcga gcgcaacgcc gccgtcctca cggacctgct cgccggcatc ccgggcgtgt
    10741 cgacgctgcc cgcaccggcg ggcctgaccc ggcgcaccta ctacgactac gtcatccgcc
    10801 tggacgcgca gctgctcggc ccggtgcccg tcgaccgcgt cgtggacgcg atgtccgccg
    10861 agctgaacat gttcttcgag accctcgaca ccccgctgaa cgccaacccg ctgtacgtgc
    10921 cgctcaagtc cccgcgcacg cccggctcgg acgaggcgcg gcgcggcctg gaccccggcc
    10981 ggttcgagct gcccgcggcg acggccgcgt accgcacctg cttcgccttc ctccaccagg
    11041 cgctgctcgg caccgagcgg gacgtgacgg cgatcgcgac cgcggtcgag aaggtcgtca
    11101 cccagctcga ccggctcggg gacggcgcgg agaggggccg ggcatgagcg tgcggctcgc
    11161 cgacggcaag gtgcgcaacc cggagggcat cgaggtcaac gccagcctcc agtgcaacat
    11221 gcgctgccag tcctgcgcgc acctctcgcc gctgtaccgc cgtgagaacg ccgacccggc
    11281 ggagatccac gacaccctga gcgtcctcgc ccggagctac cacgcctcgt acgcgaagat
    11341 catgggcggt gagccgctgc tgcacccgga cgtcgtcggc ctgatcgagg cggtgcgggc
    11401 caccggcatc tccgacacgg tcctcgtggc caccaacggc acgctcctgc accgcgcgac
    11461 ggagcgcttc tggcaggccg tggactccct ggagatctcg gtctacccga gccggatgat
    11521 cgcccccgag gagatcgagc gctaccgcgt cctggcccgg gagcacgggg tctccctcct
    11581 cgtcaactac tacgggcact tccgcgcggt gtactcggag tcgggcaccg acgcgccgga
    11641 cctggtccgg gacgtcttcg acacctgcaa gctcgcccac ttctggaact cgcacacggt
    11701 gtacgacggc tggctctacc gctgcccgca gagcgtgttc atgccccggc agctgcgcga
    11761 cggcggctgg gatccgcgcg tcgacgggct gcgcatcgag gacgacccgg cgttccttga
    11821 gcgcctgcac cgcttcctca ccgccgacga tccgctgcgg gcctgccgga actgcctggg
    11881 cagcgtcggc aagctgcatc cgcaccagga gctgccgcgc gcggggtggc aggtgacgga
    11941 gcagttggag gagctggtcg actacccgtt cctgaaggtc tgcaaggacg acatcacggc
    12001 cgacgacggc tgtgtggagc ggtcgttgtc cgcgccggtg ggaggtgcct gacatgacgg
    12061 ggaacgagaa gcccgcagcg gccggtcccg gcgagagcgt caccgtcgtc accctgaccc
    12121 gctaccggcc ggtcctcgtg cagcgggcca tgcgcagcgt ccgcgcccag gtggccgacg
    12181 tgccggtgcg ccacctcgtc ctcatcgacg acagcgagga gacgctgacc gcgctgagcg
    12241 aggtgacgga cgcgttcccg cactgcgagg tgcggtacgt gccgcgcgag ccgcacgaga
    12301 agtcaggacc ggggcggtcc tcgcgcctgc gcaacctcgg ggtgcgcctg tccgactcct
    12361 cgtggatcgc ctacctcgac gacgacaacg agtggacgcc cgaccacctc cagtccctcc
    12421 tcgaccaggc ccgctccgtg cgcgccgtct acagcgaggt cggtctcctg gacaaggacg
    12481 gcgccccgta cctggagccg cggtggccgt gggcgaacag cgtcgccgag gcggagcgga
    12541 tgtacgcgga ctacgtggcc aagggcatct gcgtaccggg ctcgaacgtc atcaaggacc
    12601 ggccgggcac gtacgaggtg cccgtcgaca ccagcgcctg gctgcttgcg cgggagctgc
    12661 tgctcgccgt gcccttcgag gagcagttct ccgccgccga cgcggacggc ctggtcagcg
    12721 aggacgacaa gctctactac cgcctcgtcc agggccggga gccgatggtg tgcacggggc
    12781 gcgacgctcc gctactacct gggcggctac tccaacagcc tcgagaccgt cacgcccgag
    12841 gagcggggcg cgatcgcctg ggcctcggag tgaggcggct tcccgacacc cgtggcgcgc
    12901 gacgacgaca gacaagggag agggttccgt ggtgaccgag tcgacgaccg cggcgggaga
    12961 ccgtcgcgct ctgggggccg agcacggctc ctaccactgg ggggacagcc tcgcgctgct
    13021 ggagggggtt cccgacggca gcgtgcgcgc ggtggtctgt tcgccgccgt tcgagggccc
    13081 gcagctcatc gccgacgagg accgcgcggg cgcgtgttcc tccgactggc tgatgccgtt
    13141 cttcgggcag ttcgagcggg tgctgcgccc ggacggctgc gtggcgttcg agctgggcgg
    13201 cctctggctg tcggacgcgc cgggcaaggc ggtgcagcac gccggtgccg tgcacgccct
    13261 ggcgaaggcg ggctggcggc tcgtgcagga cttctactac tacaacccgc agctgctcaa
    13321 cccggagccg gagggtgcgg cacgggcccc ggactccgtc acgcccatct gggtcatggc
    13381 gcgcacgcac gacgtgcact acgacgtctc agcgctgcgg catccggcgc gagcgccctt
    13441 cgtgcggggc aatctgctgg agttcgacac ctcgggcgtc tatgaccggg cgtacgagaa
    13501 gaccctggcg gaaacgtcac tgcaccccta tgtggaccgc tggcccacgg tggtgcccga
    13561 gttgttcgtg gagctgctga cccggcccgg tgatctggtc ctcgatccgt tcgcgggcag
    13621 cggggcgacc tgtttcgccg ccgagcgcct gggccggcgg tggctcggct tcgagctgga
    13681 ccggggcctg gagcagcacg tgcgggcgat gttcgccgcc cgccgcgcgg gctagcggtg
    13741 gtggtcccgt gctccggcac gggaccaccc accctttccc ggcagggccc tgacgctccg
    13801 tcaggccggg gccaggagcg cttccggcac ccggcagtcg acccaccggc cgccgtcggc
    13861 ggaggcgtag gcgccgtcga tcacgaacga ggtggtcagc gcctcccgga tcaggtcgcg
    13921 gccccgctcg ggctcggcga ccaccgccgt gaagtcgagc agcatccgct ggaaccactc
    13981 ctggtgggac gggtcgtcga agccggactc gatggacgtg cggaccacga caccgtccgc
    14041 cgcgtaggtg aggtcgtcac cgtcgacgac cacggaaccg gaggtcccca tgatcgaata
    14101 tccggtgctg cgatgacccg cggcccacga caggtgcagc gcgatctcga cgtcgtcctc
    14161 gcaccgcagc cgcagcagcg cggtgtcctc ggtgtcggcg tacgcggcgc cgtcccgcag
    14221 ccgccccgtg aggcaggaga ccgacaccgg ggtcagtcct gtcaggttca tggcaaggta
    14281 cacgctgtgc gggccgtggt cgcgcaggat gccgccgtgc gacgtgccgg gctcgcgccg
    14341 ccagtgcggc cgccagtcgg gcacgccctt ggcgtgtccg gcgcgcaggg tgcggaagtg
    14401 ggcgccgacg accttgccga agccgggtgc ggcgatgcgc tccttcatcg ccgcgagcac
    14461 gggcgcgtac ttgtagacgt ggcacgggta gtacacgcgg tcgctggcgt ggatccggcc
    14521 cagctggccg gcgtagccgt ccgcggcggg caggaagacg ggcttctcgc acaggacgtg
    14581 cagtccgtgc gcgagcgcga ggtccgagta ctcggcgtgg ctgctcgggg gtgtgcagat
    14641 gtcgacgaag tcgagggtct cgttcgccac cagcttggcg aagtcgctgt aggcgggaag
    14701 tccgaacgat tcctcgggca tgctccgccg ctccttcgag acgtcgacgt ccgcggctat
    14761 ggataagtcc tctacccgcg aatagccgac catgtgcccc atggcgatgg taccgaatcc
    14821 aatgatggct ccgcgcatgg cgaacctcct tcttcaatac ggctgccgga cgcgtgaaga
    14881 gcacgcgaga aaggtggatg cgtgcacaat ttttaagtgc catacgacgc catgtcaacc
    14941 agcggccaga attaaagagt tgcctcttcg ggctctcgat atcaaaccgg agtcgtttat
    15001 caggcacgcc cgaaagcgat gtccgggaac agttttaaag gaatatgggg gccacgtcat
    15061 gaaattgagc ccagccggtg cggccggatc gcccgcggcc gcgcgggtcg ccgtggtcgg
    15121 cggttccggg ttcatcggga ggaaccttcg caaggccctg gacgcggccg gcgtgcggac
    15181 gtgctcgttc acccggcagc gccccttcgt gcagggcggc cgggtgcacc gcgcgctgct
    15241 gcggtcgcac acggtcttct acgtggcggg cggcgtgacg cccgcgcggg cggagcgcga
    15301 accccggctc gccgcgcagg actactggaa tctgcagctc ctgctctggg ggctgcgcgg
    15361 cgcggcccac cgcccgctgg tggtcctcgc gggctcgggc gggaccgtgt acgaccccga
    15421 ggcggatccg ccgtaccggg aggacgcccc gacgcgcccg gtctccgcct acggcgcggt
    15481 gaagctcgcc caggagcggg ccctggccgg cgccgcgtgg acggccccgg tggtcctgcg
    15541 cctcgccaac gtctacgggc ccgggcagtc ggcgggcgcc gggttcgggg tgatcccgca
    15601 ctgggccgcg gcggtgcgga ccggccgggc actgacgctg atcggccgct cccggcggga
    15661 ctacgtgcac gtcgacgacg tgtgcgccgc cctgctcgcc gtgcaccggc gggccgacct
    15721 gctgcgggcg gcgcgaggac cgaccacgct gaacatcggc tccggcgtgc cgacgtcgct
    15781 cgacgagctg caccggcact tcgagcgggc ggcgggccgc gcgctcgacg tgcggcgcga
    15841 gcccgcccgg agcttcgacc ggtcggacgt gtggctggac gtggcggccg cccgcgaggt
    15901 cctcggctgg accccccgca ccggcctcgc ggacgggctc gcgggcacgc tcgccgctgt
    15961 cgccgcgccc gcccggctcc cgtcgtgacc ggccgggcgg acgccgccga actgcggcta
    16021 cgcggaggcg cgcagggccg cgcgccgctg gagcggctcc caccaggagc ggttgcccgc
    16081 gtaccaggcg acggtgtcgg cgaggccgcg ctcgaagccg acgagggggg cgtagcccag
    16141 ctccgtggcg gccttggtgc agtcgaggga gtaccgcgcg tcgtggccct tgcggtcggg
    16201 gacgcgctcg acctgctccc agccccggcc tgtcgcggcg agcaggtgcc cggtcagctg
    16261 ctcgttcgtc agctcggtgc cgccgccgat gtggtagacc tccccggccc tgccgtggcg
    16321 cagcgccagt tcgacgcccc ggcagtggtc cgacacgtgc agccagtcgc ggacgtgctg
    16381 cccgtcgccg tacagcggca ccttgccgcc gtcgaggagg cgggtgatga acagcgggat
    16441 gaccttctcg gggaactggt agggcccgta gttgttcgag cagcgggtca ccacgacgtc
    16501 catgccgtgc gtgcggtgac aggcgagcgc gagcagttcg gagcccgcct tggtggccgc
    16561 gtacggcgag ttcggcgcga gcgggctctc ctcggaccag gagcccaccg ggatcgagcc
    16621 gtagacctcg tcggtcgaca cgtgcaggaa ccggccgacc ccgtgccggc gcgccgcgtc
    16681 gagcagcagc tgcgtgccga ggacgttcgt cgtcacgaag ggggcggacc ccgtgatgga
    16741 ccggtccacg tgcgtctcgg cggcgaagtg caccaccgcg tcgtgccccg gcaccacctg
    16801 gtccaggaaa tcgccgtcgc atatgtcccc gtgcacgaag gcgtgccgtg gatggcccgc
    16861 gaccgggtcg aggttttccc gcactccggc atacgtcagc ttgtccagga cggtgatacg
    16921 gatgtcgttc gccgcgccag gggccaacag catggaccgg acaaattcgg acccgatgaa
    16981 accggcaccg cccgtcacaa taatccgcat ggagaaaaga tacacacgga cggggggacg
    17041 gcggtaggga ccccgacagg aagggaggaa agggccggtc cgcacgaacg gccggcccgc
    17101 tgtcagcaat gcgcggaatc atacttgcag gaggcaccgg ttcacgtctg tggccggtca
    17161 cccaggccgt ttccaaacag ctcatgccgg tgttcgacaa acccatggtc tattacccgc
    17221 tgtccaccct gatgctggcc ggaatccgcg acatcctcgt catcaccacc cgcgaggacg
    17281 agatcgactt ccggcggctg ctcggcgacg gctcgcacct cggcctccgg ctcacctacc
    17341 ggacgcagga ccggcccggg ggcatcgcgg aggccttcct catcggcgcc gacttcatcg
    17401 gcggcgagcc cgtggccctg atcctcgggg acaacatctt ccacggccgc agcttcggcg
    17461 tgcacgtgcg cgagcacacg gcccccgacg gcggccgcat cttcgcctac cccgtcgccg
    17521 accccacggc gtacggcgtc gtcgagatcg gcgaggacgg cacggccctg tccatcgagg
    17581 agaagcccgc caagcccaag tcccggtacg ccgccgtggg tctgtacttc tacgacgagc
    17641 acgtcgtcga catcgccgcg gggctccgcc cgagcgagcg cggcgagctg gagatcaccg
    17701 gggtcaacga ggagtacctg cggcgcggcg cgctgtccgt gacggtgctc gaccggggca
    17761 ccgcctggct cgacaccggc acgttcacgg cgatggtgca ggcctcggag tacgtgcgcg
    17821 tcatcgagga gcgccagggc ctgaagatcg gctgtgtgga ggaggtcgcg tggcgctgcg
    17881 gctacatcga cgacgagcgg ctgcgcgcgc tgagcggccc gctgctcaag agcgggtacg
    17941 ggcgctatct gctgagcctg ctcgacgagg aggcgctgtt gtgagggacg ggcacgggcc
    18001 cggcgcggtg cggctgcgcc gggcccgctg ggtgcgggcg accgcctcgg cgccgtcatc
    18061 ctccgtcggc gggtcagggg ttgatcttgg tccaggtgaa cgcgaccttg tccgccgcct
    18121 tgatgtgcag ctggcagccg ccgacctgcg tggcctcgcc gttgacggcg atgttccagt
    18181 agtacgcgtt gccgccgttg tggccgccga tggtggtcac gaatagtcgt cgaaggacgc
    18241 gtaccaggtg ccgtcccagg tgaagccctg cttcttggcg gcgtcgtcga gggccgccgt
    18301 cggggtgggc cctgccgagg tgttggcctc gttgttggtg ccgtcgcact tgtgggtcc
//
